DNA

FINGERPRINTING

B A N G O Y | B A N T E C I L | FA B R I C A N T E | T U M O N G H A
 WHAT IS DNA
FINGERPRINTING?
DNA fingerprinting is a laboratory technique
used to determine the probable identity of a
person based on the nucleotide sequences of
certain regions of human DNA that are unique
to individuals.
 WHAT IS DNA
FINGERPRINTING IN
FORENSIC?
DNA fingerprinting is used in a variety of situations, such as
criminal investigations, other forensic purposes and paternity
testing. In these situations, one aims to “match” two DNA
fingerprints with one another, such as a DNA sample from a known
person and one from an unknown person.
 HOW DOES IT WORK?
Lab workers take those strips of DNA and mix them into a gel.
Then they run an electric current through the gel, which separates
smaller strands of DNA from the larger ones. A dye added to the gel
makes the DNA strips stand out when they're placed against an
ultraviolet light or lit up with a laser
DNA Fingerprint Machines
HISTORY

Sir Alec Jeffreys

University of Leicester (UK)

In 1984, Alec Jeffreys discovered the technique of

genetic fingerprinting in a laboratory in the Department
of Genetics at the University of Leicester.
HISTORY

1985 Enderby Murders

England

The first use of DNA Fingerprinting in a criminal case

occurred in England when used to exonerate a suspect in
the Enderby Murders.
HISTORY

1995 Romanov Family

Russia

DNA Fingerprinting was used to identify the remains of

the Romanov Family in Russia.
Case
Gary Dotson Case
• 1979 (Conviction)
• Rape Case
• Microscope Matching of Pubic Hair
• 2003(Exonerated by DNA Fingerprint
 Updates
• Rapid DNA technology was introduced
• Next-Gen sequencing technologies improved the
efficiency and accuracy of DNA profiling
• GSK, Joseph James DeAngelo, was apprehended
and convicted using DNA evidence from a
genealogy website.
 What is new?
AFLP, a new technique for DNA Fingerprinting

Amplified fragment length polymorphism (AFLP) is a

PCR-based technique that uses selective amplification of
a subset of digested DNA fragments to generate and
compare unique fingerprints for genomes of interest.
Thank
you!