Preprocessing of Genotyping & scRna seq data

Checkpoint (6) 19/01/2024

Director: Pr. Lluis Quintana-Murci Co-supervisor: Dr. Maxime Rotival
Human Evolutionary Genetics Unit
Marwan Sharawy
 Annotation

• Split by condition

NS IAV COV

• NS 250k cell

• progress: 92% of cells are assigned to

one of cell type.

• 37 identified cell type

Annotation
 Annotation

ID 715

ID 918
 Wierd sample

all cells are only B memory cells 918

715
Wierd sample
More QC

100k Cutoff

removed :1100 cell

remaining :724k
 To do after annotation

• Compute Bulk expression per individual per celltype

for eQTL mapping and age & sex effects on immune
respone variablity

• Differntial expression analysis, pathway enrichment

analysis

• Correlation with FACS data

• Compositional analysis

• Ethics in science (mandatory doctoral training) Date : mardi 23 janvier