Repeated Measures ANOVA

Rogaine for Hair Growth in Women

V.H. Price and E. Menefee (1990). “Quantitative Estimation of Hair Growth I. Androgenic Alopecia
in Women: Effect of Minoxidil,” The journal of Investigative Dermatology, Vol.95, pp.683-687
 Data Description
• Subjects: 8 Women with Androgenetic Alopecia

• Treatments: Minoxidil (Rogaine) and Placebo

(4 Women per treatment)

• Time Periods: Measurements made Pre-

treatment (0 weeks), 8, 16, 24, and 32 weeks
(will only consider post-treatment measures)
 Data
Trt Subject Week0 Week8 Week16 Week24 Week32
1 1 216 290 340 275 294
1 2 130 146 206 220 209
1 3 206 193 218 223 226
1 4 106 130 144 150 173
0 5 142 154 145 160 148
0 6 178 161 170 194 169
0 7 189 219 197 218 203
0 8 180 185 223 201 182

Trt\Week 8 16 24 32 Overall
Rogaine 189.75 227 217 225.5 214.8125
Placebo 179.75 183.75 193.25 175.5 183.0625
Overall 184.75 205.375 205.125 200.5 198.9375

Subject Trt Average

1 Rogaine 299.75
2 Rogaine 195.25
3 Rogaine 215
4 Rogaine 149.25
5 Placebo 151.75
6 Placebo 173.5
7 Placebo 209.25
8 Placebo 197.75
 Mean Hair Growth versus Week by Treatment
240

230

220

210

200
Hair Growth

Rogaine
Placebo
Overall
190

180

170

160

150
0 5 10 15 20 25 30 35
Week
 Statistical Model
Yijk     i  b j ( i )   k  ( ) ik   ijk
where :
  Overall Mean Hair Growth
 i  Effect of Trt i i  1,2
b j (i )  Effect of j th woman wit hin treatm ent i j  1,2,3,4
 k  Effect of k th time period k  1,2,3,4
( ) ik  Interactio n between Trt i and Time k
 ijk  Random error term
 Sums of Squares
Total Sum of Squares (df  2(4)(4) - 1  31)
 
TSS  (290  198.94) 2  ...  (182  198.94) 2  73045.88

Treatment Sum of Squares (df  2 - 1  1) :

 
SSTRTS  4(4) (214.81  193.94) 2  (183.06  198.94) 2  8064.5
Subject Wi thin Treatment Sum of Squares (df  2(4 - 1)  6) :
SS Subjects(Trts) 
 
4 (299.75  214.81) 2  ...  (149.25  214.81) 2  (151.75  183.06) 2  ...  (197.75  183.06) 2 
 55475.88

Time Sum of Squares (df  4 - 1)  3 :

 
SSTime  2( 4) (184.75  198.94) 2  ( 205.38  198.94) 2  (205.13  198.94) 2  (200.50  198.94) 2  2267.63
Treatment x Time Interactio n Sum of Squares (df  1(3)  3) :
 
SSTrtsxTime  4 (189.75  214.81  184.75  198.94) 2  ...  (175.5  183.06  200.50  198.94) 2  2004.75
Error2 Sum of Squares (Time x Subject(Tr t) Interactio n) (df  3(6)  18) :
SS Error2  5233.13 (By Subtractio n)
 ANOVA Table/F-Tests
Source df SS MS F P-value
Treatments 1 8064.50 8064.50 0.87 0.3864
Subject(Trts) 6 55475.88 9245.98
Time 3 2267.63 755.88 2.60 0.0839
TrtxTime 3 2004.75 668.25 2.30 0.1120
Error2 18 5233.13 290.73
Total 31 73045.88

Note: We cannot conclude any treatment or time effects or interaction at

the 0.05 significance level. The subject-to-subject variation is very large,
which makes finding a treatment effect very difficult in this setting (and
very few subjects as well)
 Post-Hoc comparisons*
Comparing Treatments (1 Comparison : Rogaine - Placebo)
Y R  214.81 Y P  183.06 MS Subjects(Trt )  9246 df  6 t.05 / 2, 6  2.447
2(9246)
 214.81  183.06  2.447  31.75  83.19    51.44,114.94 
4( 4)

Comparing Time Means (6 Comparison s) :

Y ..1  184.75 Y ..2  205.38 Y ..3  205.13 Y ..4  200.5 MS Error2  290.73 t.05 /(2 x 6 ),18  2.963
2(290.73)
BTime  2.963  25.26 No time means differ by more than 21.63
2( 4)

* Bonferroni’s method for illustration only (no significant effects found from
Analysis of Variance)