Thursday 9/20 & Friday 9/21

DO NOW: What went wrong with the gel? Think of at least 3 things.

IWBAT explain how restriction enzymes work and the role of PCR

HW: Finish lab flow chart for day 3

polymerase chain reaction as a way to make multiple copies of a segment of DNA. http://www.youtube.com/watch?v=2KoLnIwoZK U

 restriction enzymes, which can be used to cut DNA into fragments of different lengths.

Restriction Enzyme
A restriction enzyme is an enzyme that cuts DNA at specific recognition nucleotide sequences. To cut the DNA, a restriction enzyme makes two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix.

Useful techniques that use restriction enzymes

DNA crime scene analysis

Production of human Insulin

Transgenic plants Production of drought tolerant rice

New Food

Dairy Cows

Fluorescent zebrafish for pollution detection

Restriction Enzyme Activity