BT-504 Mod-III NKJ Assignment No.

I Date: 4th Aug, 2011 Submit only your hand written answer Topic: DNA Replication, Due: 11 a.m. on 10th August. 2011
1. Compare conservative, semiconservative, and dispersive modes of DNA replication. 2. Describe the role of 15N in the Meselson-Stahl experiment, explain the conclusion 3. In the Meselson-Stahl experiment, which of the three modes of replication could be ruled out after one round of replication? After two rounds? 4. (a) Describe Taylor, Woods, and Hughes Experiment with diagram only and write the conclusion. (b) Predict the results of the experiment by Taylor, Woods, and Hughes if replication were (i) conservative and (ii) dispersive. 5) Describe John Cairns Experiment with diagram only and write the conclusion. 6) Describe the Okajaki experiment with diagram only. Write the conclusion. 7. What are the requirements for in vitro synthesis of DNA under the direction of DNA polymerase I? 8. In Kornberg's initial experiments, it is rumored that he grew E. coli in Anheuser-Busch beer vats. (He was working at Washington University in St. Louis.) Why do you think this was helpful to the experiment? 9. How did Kornberg assess the fidelity of DNA by polymerase I in copying template DNA? 10. Which characteristics of DNA polymerase I raised doubt that its in vivo function is the synthesis of DNA leading to complete replication?