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Differences between SEM & TEM

TEM 1. TEM (transmission electron 1. SEM (scanning electron microscope/microscopy) also refers to microscope/microscopy) refers to the the instrument and the method used instrument and the method used in in electron microscopy. electron microscopy. 2. The method used in SEM is based on scattered electrons while TEM is based on transmitted electrons. The scattered electrons in SEM are classified as backscattered or secondary electrons. 3. The scattered electrons in SEM produced the image of the sample after the microscope collects and counts the scattered electrons. 4. SEM focuses on the sample#s surface and its composition 5. SEM also shows the sample bit by bit 6. SEM also pro ides a three$ dimensional image 7. SEM only offers % million as a ma&imum le el of magnification. 8. SEM has (.) nanometers 2. Howe er! there is no other classification of electrons in TEM. SEM

3. "n TEM! electrons are directly pointed toward the sample. The electrons that pass through the sample are the parts that are illuminated in the image. 4. TEM seeks to see what is inside or beyond the surface. 5. TEM shows the sample as a whole 6. TEM deli ers a two$dimensional picture. 7. TEM has up to a '( million magnification le el 8. The resolution of TEM is (.' angstroms 9. SEM images ha e a better depth of 9. TEM images ha e a less depth of field field 10. The sample in SEM is cut less 10. The sample in TEM is cut thinner thinner 11. SEM sample is *stained+ by an 11. SEM sample is not *stained+ element that captures the scattered electrons 12. "n SEM! the sample is prepared on 12. TEM re0uires the sample to be speciali,ed aluminium stubs and prepared in a TEM grid and placed in placed on the bottom of the chamber the middle of the speciali,ed chamber of the instrument. The image of the of the microscope. The image is sample is pro-ected onto the ./T or produced by the microscope ia tele ision$like screen. fluorescent screens.

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Amit Verm .

Department of Pharmaceutical Sciences 3