Enzyme investigation

a) By using distilled water: In order to maintain fair rather than biased results from other
contaminating chemicals that are in water
b) Kept at 37oc in a water bath: It was kept at this exact temperature in a water bath in order for a
chemical reaction taking place
c) Left for 30 mins: In order to wait and observe the reactions which occur during this determined
amount of time.
d) After 10 mins: They had to wait for ten minutes before having to add chemical into the test tube
in order to make results fair and as correct a possible.

b) We used buffer solutions as a method of getting rid of any contaminating chemicals or
substances which could variate our results and also to maintain the level of concentration inside
each test tube for fair data.
c) Close observations made by each of us could of helped to see if starch was present or not (if
there was presence of a orange- yellowy colour, this meant that starch was present).



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PH number
Achromatic time /s Vs. PH number
Achromatic time /s
Expon. (Achromatic
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