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  • Titrasi Asam Glutamat Dengan HCL

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    abdinaufal
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    1. The document describes experiments to determine the pKa values of glutamic acid and glycine through acid-base titrations with HCl and NaOH. The pKa values were found to be 1.186 and 6.299 for glutamic acid, and 2.061 and 8.712 for glycine. 2. Experiments were conducted to generate a standard curve for protein concentration determination using the Biuret method. The standard curve equation was determined to be y = 2E-05x + 0.2245 with R2 = 0.9475. 3. Calculations were shown to determine the casein concentration in milk as 10.5 grams from a 100 mL sample, comprising 10

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    Lampiran Perhitungan

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    1. The document describes experiments to determine the pKa values of glutamic acid and glycine through acid-base titrations with HCl and NaOH. The pKa values were found to be 1.186 and 6.299 for glutamic acid, and 2.061 and 8.712 for glycine. 2. Experiments were conducted to generate a standard curve for protein concentration determination using the Biuret method. The standard curve equation was determined to be y = 2E-05x + 0.2245 with R2 = 0.9475. 3. Calculations were shown to determine the casein concentration in milk as 10.5 grams from a 100 mL sample, comprising 10

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    15 views4 pages

    Titrasi Asam Glutamat Dengan HCL

    Uploaded by

    abdinaufal
    1. The document describes experiments to determine the pKa values of glutamic acid and glycine through acid-base titrations with HCl and NaOH. The pKa values were found to be 1.186 and 6.299 for glutamic acid, and 2.061 and 8.712 for glycine. 2. Experiments were conducted to generate a standard curve for protein concentration determination using the Biuret method. The standard curve equation was determined to be y = 2E-05x + 0.2245 with R2 = 0.9475. 3. Calculations were shown to determine the casein concentration in milk as 10.5 grams from a 100 mL sample, comprising 10

    Copyright:

    © All Rights Reserved
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    of 4

    Lampiran Perhitungan

    1. Kurva Titrasi Asam-asam Amino


    - Titrasi asam glutamat dengan HCl

















    Perpotongan garis:
    y
    1
    = y
    2

    -1,13x + 3,3 = -0,020x + 1,983
    1,11x = -1,317
    x = 1,186 pKa
    1

    - Titrasi asam glutamat dengan NaOH

















    Perpotongan garis:
    y
    1
    = y
    2

    0,039x + 10,88 = 1,007x + 4,782
    -0,968x = -6,098
    x = 6,299 pKa
    2


    y = -1.13x + 3.3
    R = 1
    y = -0.0206x + 1.9837
    R = 0.8664
    0
    0.5
    1
    1.5
    2
    2.5
    3
    3.5
    0 10 20 30 40
    p
    H

    l
    a
    r
    u
    t
    a
    n

    Volum penambahan HCl (mL)
    Titrasi Asam Glutamat dengan HCl
    Series1
    Series2
    Linear (Series1)
    Linear (Series2)
    y = 1.0075x + 4.7825
    R = 0.8635
    y = 0.0399x + 10.881
    R = 0.8496
    0
    2
    4
    6
    8
    10
    12
    14
    0 10 20 30 40
    p
    H

    l
    a
    r
    u
    t
    a
    n

    Volume penambahan NaOH (mL)
    Titrasi Asam Glutamat dengan NaOH
    Series1
    Series2
    Linear (Series1)
    Linear (Series2)
    Titik isoelektrik asam glutamat:



    - Titrasi glisin dengan HCl

















    Perpotongan garis:
    y
    1
    = y
    2

    -3,075x + 8,411 = -0,037x + 2,150
    -3,038x = -6,261
    x = 2,061 pKa
    1

    - Titrasi glisin dengan NaOH














    Perpotongan garis:
    y
    1
    = y
    2

    0,046x + 10,93 = 0,310x + 8,630
    -0,264x = -2,3
    x = 8,712 pKa
    2

    Titik isoelektrik glisin:


    y = 0.3104x + 8.6304
    R = 0.9691
    y = 0.0461x + 10.93
    R = 0.9114
    0
    2
    4
    6
    8
    10
    12
    14
    0 5 10 15 20 25 30
    p
    H

    l
    a
    r
    u
    t
    a
    n

    Volume penambahan NaOH (mL)
    Titrasi Glisin dengan NaOH
    Series1
    Series2
    Linear (Series1)
    Linear (Series2)
    y = -3.075x + 8.4117
    R = 0.9793
    y = -0.0374x + 2.1501
    R = 0.7287
    0
    1
    2
    3
    4
    5
    6
    7
    8
    9
    10
    0 5 10 15 20 25
    p
    H

    l
    a
    r
    u
    t
    a
    n

    Volume penambahan HCl (mL)
    Titrasi Glisin dengan HCl
    Series1
    Series2
    Linear (Series1)
    Linear (Series2)
    2. Penentuan Kadar Protein secara Biuret
    - Kurva baku





























    Prosentase tiap protein:
    a. Kasein
    []


    = []

    L


    L






    b. Pepton
    []


    = []

    L


    L

    No x (ppm) y (A) x
    2
    x.y
    1 1000 0.251 1000000 251
    2 3000 0.279 9000000 837
    3 5000 0.308 25000000 1540
    4
    8000 0.356
    64000000 2848
    5 9000 0.412 81000000 3708

    180000000 9184
    y = 2E-05x + 0.2245
    R = 0.9475
    0
    0.05
    0.1
    0.15
    0.2
    0.25
    0.3
    0.35
    0.4
    0.45
    0 2000 4000 6000 8000 10000
    a
    b
    s
    o
    r
    b
    a
    n
    s
    i

    konsentrasi (ppm)
    Kurva Baku




    c. Gelatin
    []


    = []

    L


    L





    Jadi persen tiap protein :























    3. Isolasi Kasein dari Susu
    w[kasein] = 10,5 gram
    w[susu] = volume susu susu
    = 100 mL 1,032 g/mL = 103,2 gram


    []
    []






    Protein % protein
    Kasein 0,202
    Pepton 0,094
    Gelatin 0,527
    y = 2E-05x + 0.2245
    R = 0.9475
    0
    0.05
    0.1
    0.15
    0.2
    0.25
    0.3
    0.35
    0.4
    0.45
    0 2000 4000 6000 8000 10000
    a
    b
    s
    o
    r
    b
    a
    n
    s
    i

    konsentrasi (ppm)
    Kurva Baku

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