B.Sc.

Biotechnology
Semester IV
BBT 411: Molecular Biology II
1

Total

DNA replication
DNA polymerases: types and structures, priming reactions, creating
replication fork, semiconservative replication of DNA, synthesis of leading
and lagging strands, Okazaki fragments, termination of replication, telomere
replication.
DNA repair
Damage to DNA, proof reading and repair mechanisms (excision, mismatch,
recombination), SOS response.
Prokaryotic transcription
RNA polymerase: structure, role, promoter recognition, sigma factor,
initiation, elongation and termination.
Eukaryotic transcription
Eukaryotic RNA polymerases: types, promoters, TBP, enhancers and
transcription factors, initiation of transcription, posttranscriptional
modifications of RNA.
Translation
Role of ribosomes and tRNA in protein synthesis, initiation, formation of
peptide bonds, translocation, elongation and termination of protein
synthesis.
Gene regulation
Prokaryotic and eukaryotic genes, operon concept ( lac and trp operon)
References
1. Biochemistry, 5th Edn., Berg, J., Tymcoczko, J. and Styrer, L. W.H
Freeman and Co., New York (2002).
2. Molecular Biology of the gene, Watson, J., Stephen, Dorling
Kindersley (India) Pvt. Ltd., New Delhi (2004).
3. Genes IX, Lewin, B., Jones and Bartlett Publ., Sudbury (2008).

45 L
9L

6L

8L

9L

9L

4L