Kristy Soltmann

ELD 376
Professor Gallo
February 23, 2015
Life Science Lesson
Do Hand Soaps and Sanitizers Prevent the Growth of Bread Mold?
Materials
Fresh bread- 8 slices

Plastic sandwich bags- 16

Latex gloves

Labels

Pen/marker

Toaster (In the teachers lounge)

Knife- butter knife

Spray bottle

Baking sheet

Camera

Measuring teaspoon

Liquid Soap

Hand Sanitizer

Procedures
1.
2.
3.
4.

First, wash all hands and materials. Put on all safety gear.
Take out the 4 toasted pieces of bread.
Next, I will cut all 8 of the bread slices in half. 16 pieces total: 8 toasted and 8 untoasted.
Each group of students will be given two slices of bread.
You will be creating specimens in sandwich baggies according to the following chart:
Bag Number

Sealed?

Toasted?

Treatment:

Sealed

Toasted

None

Sealed

Toasted

Water only

Sealed

Toasted

Hand Soap only

Sealed

Toasted

Hand Sanitizer only

5.
6.
7.
8.
9.

11.
12.
13.
14.
15.
16.
17.
18.

Sealed

Untoasted

None

Sealed

Untoasted

Water only

Sealed

Untoasted

Hand Soap only

Sealed

Untoasted

Hand Sanitizer only

Unsealed

Toasted

None

10

Unsealed

Toasted

Water only

11

Unsealed

Toasted

Hand soap only

12

Unsealed

Toasted

Hand Sanitizer only

13

Unsealed

Untoasted

None

14

Unsealed

Untoasted

Water only

15

Unsealed

Untoasted

Hand Soap only

16

Unsealed

Untoasted

Hand Sanitizer only

1 piece of untoasted bread goes into a sandwich baggie; sealed and labeled untoasted,
sealed.
Next, 1 piece of the toasted bread will be put into a sandwich baggie and sealed it. The
bag will be labeled it as toasted, sealed.
Next, 1 piece of untoasted bread in a sandwich baggie and leave it unsealed. Label it as
untoasted, unsealed.
Put 1 piece of toasted bread in a sandwich bag and leave it unsealed. Label it as toasted,
unsealed.
Fill the spray bottle with water.
10. Put 2 pieces of toasted bread and 2 pieces of untoasted bread into their own sandwich
bags.
Spray each piece lightly with water.
Close 1 of the toasted bags and 1 of the untoasted bags, leaving the other two unsealed.
Label each bag appropriately (for example: untoasted, unsealed, water).
Add 1 teaspoon of soap to the water in the spray bottle. Mix well.
Put each of 2 pieces of toasted bread and each of 2 pieces of untoasted bread into their
own sandwich bags.
Spray each piece lightly with the soapy water.
Close 1 of the toasted bags and 1 of the untoasted bags, leaving the other two unsealed.
Label each bag appropriately (for example: sealed, toasted, soap)
Dump out the soapy water in the spray bottle and wash the bottle well. Fill it again with
water and add 1 teaspoon of hand sanitizer. Mix well.
Put each of 2 pieces of toasted bread and each of 2 pieces of untoasted bread into their

19.
20.
21.
22.
23.
24.

own sandwich bags.

Spray each piece lightly with the hand sanitizer-water mixture.
Close 1 of the toasted bags and 1 of the untoasted bags, leaving the other two unsealed.
Label each bag appropriately (for example: sealed, untoasted, hand sanitizer).
Lay out all of the bags on the baking sheet, using the spray bottle spray-side up. Be sure
the labels are visible.
Place the tray in a warm, dark place. (In the classroom cabinet.)
After 1 week, seal all of the open bags. Be sure not to open any of the bags again once
mold starts to grow.
Take notes and pictures over the next 21 days. The students will also be able to record by
drawling and coloring the pictures in their journals.
25. After youve completed your experiment, dispose of the bread, keeping the bags
sealed.
Closure
The students will be given an exit card after the study is over. The students will be given
a worksheet, asking the students to identify different types of mold they have found
during this study. The students will hand in the exit cards when completed.