Lecturer: Huynh Ngoc Oanh

Name of student:
1.
2.

Huynh Hong Nhung (AHD13134)

Phan Vinh Dong Phuong (AHD13154)

Class: CT13AHD - Thursday Morning

Group: 6

LESSON 1 : DETERMINATION OF SUGAR AS GLUCOSE

I- REACTION EQUATION

Determine the amount of sugar or carbohydrates in a soft drink by a spectrophotometric method.

3,5-dinitrosalicylic acid (DNSA) 3-amino-5-nitrosalicylic acid

II- PROCEDURE

III- RESULT

V
C(M)
VHCl
(mL)
VNaOH
(mL)

VDNSA
(mL)

C0
0
0

C1
2

C2
2

C3
2

C4
2

S1
2
-

S2
2
-

S3
2
-

T%
A
_ Beers Law Equation:
_ Concentration of dilute Sample: S1
S2
S3
_ Concentration of the soft drink:

IV- COMMENT
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V- OTHER METHOD

Shaffer-Hartmann method: An obsolete method for the quantitative determination of

glucose in biologic fluids, based on the reduction of copper by the reducing group of the
sugar.