ACTIVITY NO.

13 - "LARA"
QUESTION:
1. What is meant by specific rotation?
> specific rotation ([]) is a property of a chiral chemical compound. It
is defined as the change in orientation of monochromatic plane-polarized light,
per unit distanceconcentration product, as the light passes through a sample of
a compound in solution.
https://en.wikipedia.org/wiki/Specific_rotation

2. Why is the hydrolysis if sucrose called inversion? Write the equation for the
inversion of sucrose.
> compound, colourless, sweet-tasting crystals that dissolve in water. Sucrose
(C12H22O11) is a disaccharide; hydrolysis, by the enzyme invertase, yields invert
sugar (so called because the hydrolysis results in an inversion of the rotation of
plane polarized light), a 50:50 mixture of fructose and glucose, its two constituent
monosaccharides....
>
http://www.britannica.com/topic/invert-sugar

3. What is the purpose of adding NaOH to the mixture before taking the polarimeter
reading?
> To neutralize the solution and to prevent the complete hydrolysis of sucrose
into glucose and fructose.
http://www.answers.com/Q/Purpose_of_adding_NaOH_to_the_mixture_before_t
aking_the_polarimeter_reading

4. How is sucrose hydrolyzed in the body?

> n humans and other mammals, sucrose is broken down into its constituent
monosaccharides, glucose and fructose, by sucrase or isomaltase glycoside

hydrolases, which are located in the membrane of the microvilli lining the
duodenum.
https://en.wikipedia.org/wiki/Sucrose

5. Calculate the amount of glucose in 10 ml. Of a solution which gives an equilibrium

rotation of +26. 1 deg. In a 200 mm. Polarimeter tube.