Professional Documents
Culture Documents
SOCIETASBIOLOGORUMCROATICA
CroatianBiologicalSociety
12.HRVATSKIBIOLOKIKONGRES
smeunarodnimsudjelovanjem
th
12 CROATIANBIOLOGICALCONGRESS
withInternationalParticipation
SvetiMartinnaMuri,18.23.IX2015.
ZBORNIKSAETAKA
BOOKOFABSTRACTS
Hrvatskobiolokodrutvo
SOCIETASBIOLOGORUMCROATICA
CroatianBiologicalSociety
12.HRVATSKIBIOLOKIKONGRES
smeunarodnimsudjelovanjem
18.23.rujna2015.
SvetiMartinnaMuri,Hrvatska
th
12 CROATIANBIOLOGICALCONGRESS
WithInternationalParticipation
18th23rdSeptember2015
SvetiMartinnaMuri,Croatia
ZBORNIKSAETAKA
BOOKOFABSTRACTS
Zagreb,2015.
ZBORNIKSAETAKA
12.HRVATSKOGBIOLOKOGKONGRESA
BOOKOFABSTRACTS
OFTHE12thCROATIANBIOLOGICALCONGRESS
Urednici/Editors
GranKlobuar
NevenkaKopjar
MarijaGligoraUdovi
aklinLuka
DuanJeli
Odgovornitehnikiurednici/TechnicalEditorsinChief
GranKlobuar
NevenkaKopjar
Hrvatskobiolokodrutvo
CroatianBiologicalSociety
Zagreb,2015.
ISSN18485553
Kljuninaslov:Zborniksaetaka(Hrvatskibiolokikongressmeunarodnim
sudjelovanjem)
Skraenikljuninaslov:Zb.saet.(Hrvat.biol.kongr.meunar.sudjel.)
ii
Organizatorkongresaiizdavazbornika/OrganiseroftheCongressandPublisherofthe
BookofAbstracts
Hrvatskobiolokodrutvo
CroatianBiologicalSociety
Rooseveltovtrg6,HR10000Zagreb,Hrvatska
Tel:+385(0)14877733;Fax:+385(0)14826260
email:info@hbdsbc.hr
URL:www.hbdsbc.hr
Organizacijskiiprogramskiodbor/OrganisingandProgramCommittee:
Gran Klobuar (Predsjednik / President), Vinja Besendorfer (Dopredsjednica /
Vicepresident),MarijaGligoraUdovi(Tajnica/Secretary),Anamariatambuk(Blagajnica
/ Treasurer), Biljana Balen, Nataa Bauer, Sunica Bosak, Domagoj iki, Mirna urkovi
Perica,DamjanFranjevi,BojanHamer,NenadJasprica,PetraKora,PetarKrui,Mladen
Kuini,BojanLazar,aklinLuka,Nenad Malenica,MarkoMilia,BoenaMiti,Anelka
PlenkoviMoraj,AnaPrevii,SandraRadiBrkanac,Dijanakori
Znanstveniodbor/ScientificCommittee:
Nevenka Kopjar (Predsjednica / President), Dunja LeljakLevani (Dopredsjednica / Vice
President), Andreja AmbrioviRistov, Renato Batel, Vesna Benkovi, Branimir K.
Hackenberger,KrunoslavBriKosti,VeraCesar,Markoaleta,Helenaetkovi,Zdravko
Dolenec, Jakov Duli, Jerka Dumi, Hrvoje Fulgosi, Sanja Gottstein, Bojan Hamer,
Dubravka Hranilovi, Jasna Hrenovi, Stipan Jonji, Mladen Kerovec, Marcelo Kovai,
Valter Koul, Stjepan Krmar, Marijana KrsnikRasol, Gordana LackoviVenturin, Gordan
Lauc, Bojan Lazar, Zlatko Liber, aklin Luka, Zrinka Ljubei, Renata Matonikin Kepija,
Melita Mihaljevi, Milorad Mrakovi, Toni Nikoli, Nadica Oroli, Mirjana Pavlica,
MiroslavPlohl,MartinaPodnarLei,JasnaPuizina,InesRadanovi,MarySopta,Zdenko
Tkalec, Zoran Tadi, Nikola Tvrtkovi, urica Ugarkovi, eljka VidakoviCifrek, Damir
Vilii,KristianVlahoviek,NedoVrgo,DavorZahradka,IrinaZupan
iii
Tehnikapotpora/Technicalsupport:
IvanaBonjak,RomanaGraan,RenataHorvat,MarijaIvkovi,MielJeli,Maja
Mejdandi,DoroteaPolovi,Majarut,VedranVukovi,Petarutini
Tehnikiorganizatorkongresa/Technicalsupportforregistration,accommodationand
excursions:
PBZCardd.o.o.,putnikaagencija/Travelagency;Radnikacesta44,10000Zagreb,
Croatia
Pokrovitelji/Patrons:
Ministarstvoznanosti,obrazovanjaisportaRepublikeHrvatske/TheMinistryofScience,
EducationandSportofRepublicofCroatia
HrvatskaAkademijaznanostiiumjetnosti/CroatianAcademyofSciencesandArts
Agencijazaodgojiobrazovanje/EducationandTeacherTrainingAgency
PrirodoslovnomatematikifakultetSveuilitauZagrebu/FacultyofScience,University
ofZagreb
BiolokiodsjekPrirodoslovnomatematikogfakultetaSveuilitauZagrebu/Department
ofBiology,FacultyofScience,UniversityofZagreb
Meimurskaupanija/Meimurjecounty
Sponzori/Sponsors:
ALFAd.d.Zagreb
Bioinstitutd.o.o.akovec
Biosistemid.o.o.Zagreb
Biovitd.o.o.Zagreb
GoreaPlusd.o.o.SvetaNedjelja
Hrvatskevode
INELmedicinskatehnikad.o.o.Zagreb
Kefod.o.o.Zagreb
Merckd.o.o.Zagreb
Nastavnizavodzajavnozdravstvodr.AndrijatamparZagreb
RUVEd.o.o.Zagreb
kolskaknjigad.d.Zagreb
ZalobaHartLjubljanaSlovenija
iv
Cijenjenekolegiceikolege,dragibiolozi!
Za one rijetke koji iz nekog samo njima poznatog razloga itaju predgovore u knjigama
saetakaelimnapomenutidasuvremenasveteazaorganiziranjekongresaapoglavito
kongresakojisuovakosveobuhvatnikaotojetoHrvatskibiolokikongres.Uovom12om
po redu Kongresu smanjili smo broj tema kako bi objedinili sudionike kongresa sa slinim
znanstvenim interesima te smo poveali broj simpozija koji se odravaju u sklopu
kongresa. Takoer, odluili smo se za Sveti Martin na Muri sa eljom da istaknemo
prirodne vrijednosti kontinentalne Hrvatske a posebice Meimurja. Pretpostavljam da je
to imalo odreeni utjecaj na vau odluku o sudjelovanju na 12. Hrvatskom biolokom
kongresu jer ovaj Zbornik saetaka sadri 8 plenarnih i 5 pozvanih predavanja te 125
usmenihi143posterskapriopenja.elimstogazahvalitisvimsudionicimaovogKongresa,
a posebice onima koji itaju ove predgovore, na odluci da odvoje vrijeme i odreena
financijskasredstvakakobisudjelovalinaovomvetradicionalnomkongresukoji,siguran
sam,imaposebnomjestounaimsrcima.
UimeOrganizacijskogiprogramskogodbora
GranKlobuar
Esteemedcolleagues,dearbiologists!
Forthoseofyouwho,forsomeunknownreasons,readforewordsinBooksofAbstractsI
wishtoemphasizethattimesarenotfavourablefororganisingcongressesandmostofall
those that are so comprehensive as CroatianBiological Congress. In this twelfth CBCwe
reducedthenumber ofcongresstopicsinordertobringtogetherbiologistswithsimilar
scientificinterestsandweincreasedthenumberofsatelliteSymposia.Also,wedecidedto
hold this Congress at Sveti Martin na Muri in order to accentuate values of continental
Croatia and especially Meimurje region for us biologists. I suppose that these changes
th
might have had some influence on your decision to attend 12 Croatian Biological
CongressasthisBookofAbstractscontain8plenaryand5invitedlectures,125oraland
143 poster presentation. Therefore, I wish to thank all the participants of this Congress,
especiallythosewhotooktimetoreadthisforeword,forthedecisiontoinvesttheirtime
andcertainfundstoparticipateinthisalreadytraditionalCongresswhichhas,Iamsure,a
specialplaceinourhearts.
OnbehalfoftheOrganisingandProgrammeCommittee
GranKlobuar
Potovanekolegiceikolege,sudionici12.Hrvatskogbiolokogkongresa!
Biologija21.stoljeasuoavasesabrojnimizazovimarazvojiprimjenanovihtehnologija
mijenjaju mnoga saznanja o ivom svijetu na svim razinama organizacije, a otvaraju se i
novapodrujaistraivanja.Premdaoduvijekvrlodinaminaznanost,suvremenabiologija
danasmodabreivienegoikadadolazidovrijednihspoznaja,kojepreispitujuipokreu
nova istraivanja ak i u okvirima "klasinih" biolokih disciplina. Biolozi su i dalje vrlo
vaan i nezaobilazan dio sloenih istraivakih timova i u razliitim srodnim znanostima.
Poveana koliina novih znanja i injenica stavlja izazove i pred biologe koji se bave
obrazovanjem,poeviodosnovnihkolapadovisokokolskihustanova,kojimorajunai
novarjeenjaiputovekojimaetanovaznanjatoboljeprenijetinadananjegeneracije
uenikaistudenata.
Jednaodnajvanijihpretpostavkinakojimasezasnivasuvremenaznanostjestpotrebaza
to brim i dinaminijim protokom informacija. Znanstveni skupovi stoga postaju vrlo
vana mjesta susreta strunjaka i istraivaa koji aktivno komuniciraju rezultate svojih
istraivanja i imaju mogunost meusobnog upoznavanja te boljeg povezivanja radi to
smislenijeisvrsishodnijeorganizacijenekihbuduihistraivakihprojekataisuradnja.
Hrvatsko bioloko drutvo kroz organizaciju svojeg dvanaestog kongresa uspjeno
nastavlja dugogodinju tradiciju okupljanja domaih, ali i inozemnih znanstvenika i
strunjaka koji aktivno djeluju u podrujubiologijeisrodnihznanstvenih grana. Nadamo
sedae,svojimbogatimznanstvenimprogramom,ovogodinjikongresispunitioekivanja
sudionikajersuzastupljenasvapodrujaodinteresanaihbiologa,akrozspecijalizirane
simpozije moi e se dobiti dobar uvid u najnovija istraivanja i suvremena kretanja u
pojedinimbiolokimdisciplinama.
Izborom plenarnih predavanja takoer smo nastojali istaknuti neka od trenutano
najvanijihivrloaktivnihpodrujauokvirubiologije,ijivrijednirezultatidovodedonovih
i kljunih spoznaja u funkcioniranju ivog svijeta i nude smjerove prema nalaenjima
odgovoranamnoge,jouvijekneistraene,biolokefenomene.
Zadovoljnismotoje12.Hrvatskibiolokikongresokupiovelikibrojbiologasvihdobnih
skupina i to su se studenti biologije kroz prijavu rezultata svojih istraivanja ve sada
aktivnoukljuiliuistraivakiprostorjerjetonajboljiputzadaljnjinapredakbiologijeu
okvirimahrvatske,aliisvjetskeznanostitedobarpoetakzanjihovosobniprofesionalni
razvoj. Takoer moramo istaknuti da ovogodinji kongres ima i naglaen meunarodni
karakterkrozvelikibrojprijavljenihsudionikaizinozemstva,toupuujenainjenicudae
naiskupovi ubudue vjerojatno prerastinacionalneokvire. To ezasigurno utjecati i na
organizacijukongresabiologaubudunosti,dajuiimnovukvalitetukakouznanstvenom,
takoistrunomsmislu.
Ovaknjigasadrisveurokupristiglesaetkeusmenihiposterskihpriopenjaprijavljenih
za12.Hrvatskibiolokikongres.Znanstveniodborproveojerecenzijskipostupakisaetke
tehniki uredio i ujednaio prema zadanom predloku. Nadamo se da e itatelji u ovoj
knjizi saetaka nai korisne informacije i poticaj za svoj znanstveni i struni rad, a svim
sudionicima kongresa elimo uspjean rad i to bolju prezentaciju rezultata svojih
istraivanjana12.Hrvatskombiolokomkongresu.
UimeZnanstvenogodbora12.Hrvatskogbiolokogkongresa
NevenkaKopjar,PredsjednicaZnanstvenogodbora
vi
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Esteemedcolleagues,dearparticipantsofthe12 CroatianBiologicalCongress!
st
Thefieldofbiologyinthe21 centuryisfacedwithmanychallengesthedevelopment
and application of new technologies has greatly affected our knowledge regarding the
livingworldatalllevelsoforganizationanduncoveredmanynewandinterestingresearch
areas. Although always a very dynamic field of study, modern biology is probably more
efficientandfasterinacquiringvaluableinformationnowthanever.Thisleadstocritical
reexaminations and initiations of new research, even within more "classical" biological
disciplines.Biologistsarestillaveryimportantandvitalpartofcomplexresearchteamsin
different related scientific fields. The increased amount of new knowledge also brings
challengesforbiologistsinvolvedineducation,startingfromprimaryschooltouniversity
level. They now have to find new solutions and ways to present this new knowledge to
theirstudents.
One of the most important assumptions that underlie modern science is the need for a
faster and more dynamic flow of information. Conferences have therefore become an
important place for meeting researchers and experts who communicate the results of
theirresearchandengageinfruitfuldiscussions,leadingtojointresearchprojects.
th
Byorganizingits12 congress,theCroatianBiologicalSocietysuccessfullycontinuesthe
longstanding tradition of joining scientists and experts active in the field of biology and
related scientific fields. We hope that the enticing scientific program of this year's
congresswillmeettheexpectationsoftheparticipantsbecauseitwasdesignedtocover
almostallareasofinterest.Throughspecializedsymposia,theparticipantswillbeableto
getgoodinsightintothestateoftheartresearchtrendsindifferentbiologicaldisciplines.
Throughourselectionofplenarylectures,wetriedtostresssomeofthemostimportant
andhighlyactiveareaswithinbiologytoday.Thevaluableresultstheyhaveprovidednew
directionstowardsfindinganswerstothemanystillunexploredbiologicalphenomena.
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We are pleased that the 12 Croatian Biological Congress brought together a large
number of biologists of all ages, especially so many biology students. By presenting the
resultsoftheirresearch,theywillcommencetheirownactiveinvolvementintheworldof
trueresearch.Thisyear'scongressalsoboastsamoreinternationalcharacterwithmany
internationalparticipants,whichpointstothefactthatourfuturemeetingswillprobably
outgrow only Croatia as their venue. This will certainly affect the organization of the
biological congress in the future, giving them a new quality both scientifically and
professionally.
th
This Book of Abstracts contains all abstracts submitted for the 12 Croatian Biological
Congress. The Scientific Committee tried, within the best of its capability, to edit the
received abstracts and conducted a peer review process. We hope that our readers will
findusefulinformationinthisBookanddeemithelpfulintheirscientificandprofessional
th
work. We would also like to wish all of the participants of the 12 Croatian Biological
Congressasuccessfulparticipationandpresentationoftheirfindings.
th
OnbehalfoftheScientificCommitteeofthe12 CroatianBiologicalCongress
NevenkaKopjar,PresidentoftheScientificCommittee
vii
viii
SADRAJ
CONTENTS
v
Predgovor/Foreword
PLENARNAPREDAVANJA/PLENARYLECTURES
Uvodnoplenarnopredavanje/Introductoryplenarylecture:
PL1
2
I.tagljar
Proteininteractionnetworksinhealth&disease
PL2
2
R.Bakari,T.DomazetLoo
Theriseandfallofgenomecomplexityandothermacroevolutionarystories
PL3
3
P.Nosil
Divergenceandconstraintintheoriginofnewspecies
PL4
3
A.orgo
Socioscientificissues:achallengeforbiologyteachers
PL5
4
D.BarZvi
Plantresponsetoabioticstress
PL6
4
A.Herrel
Analysesofmorphology,physiology,andmicrobialdiversityafterarecentdietaryswitchinalizard
PL7
5
D.Drobne
Howmuchroomisleftforconventionalbiologyinmodernnanoscaleandomicsresearch
Zavrnoplenarnopredavanje/Closingplenarylecture:
PL8
5
G.Lauc
Glycansasintegratorsofgenesandenvironmentanoftenignoredlayerofbiologicalcomplexity
USMENAIZLAGANJA/ORALPRESENTATIONS
Konzervacijskabiologija,zatitaprirodeiokolia
Conservationalbiology,natureandenvironmentprotection
O1
8
I.Sui,L.Katui
Vrijednost faunistikih istraivanja kao temelja za planiranje aktivne zatite prirode projekt
integracijeuEUNatura2000(NIP)
FaunisticresearchvaluesasthebaselineforplanningactivenatureconservationEUNatura2000
IntegrationProject(NIPproject)
th
12.HBKx12 CBC
9
O2
A.tefan,S.Stolton,D.Porej,K.Z.Ivani
AssessingprotectedareavaluesandbenefitsintheDinaricarc
O3
9
E.Patev,M.Grgurev,A.Avdakovi,M.Masla,A.Bukovac,H.Peternel
Prvaprimjenaradarskeornitologijenapodrujubalkanskeregije
ThefirstapplicationoftheradarornithologyintheBalkanregion
O4
10
D.Hamidovi,S.Desnica
MeunarodnovanapodzemnasklonitazaimieHrvatskojprimjenauouvanjuizatiti
Internationallyimportantundergroundsitesforbatsconservationandprotectionapplication
O5
11
D.K.Hackenberger,T.Vondrak
Sustavzamultispektralnuanalizuvegetacijezabiolokaiekolokaistraivanja
Multispectralvegetationanalysissystemforbiologicalandecologicalresearch
O6
12
M.Stankovi
EndemicandrelictsspeciesinspecialnaturereserveZasavica
O7
13
M.Masla,A.Partl,Z.Mesi,O.Antoni
Liajevikaobioindikatorikvalitetezrakanapodrujumotovunskeume
LichensasbioindicatorsofairqualityintheMotovunforestarea
O8
14
A.Vuci,D.PeroPucar,B.Miti,I.Hrga,B.Pucar
Utjecaj meteorolokih parametara na varijacije dvosatnih koncentracija peludnih zrnaca empresa
(Cupressaceae),gradZadar,Hrvatska
MeteorologicalinfluenceondiurnalCupressaceaepollenvariation(Zadar,Croatia)
O9
15
J.Freyhof,S.Weiss,A.Adrovi,M.aleta,A.Dupli,B.Hraovec,B.Kalamuji,Z.Mari,D.Miloevi,
M.Mrakovi,D.Mrdak,M.Piria,P.Simonovi,S.ljuka,T.Tomljanovi,D.Zabric
Mladica(Huchohucho)naBalkanu:rasprostranjenostibuduiutjecajihidroenergetskihplanova
Thehuchen(Huchohucho)intheBalkanregion:distributionandfutureimpactsbyhydropowerdevelopment
O10
16
D.Pithart,A.Dupli,P.Kutlea,I.PetrovRani,N.Trenc
ProcjenauslugaekosustavanapodrujuDravaMolveprematripotencijalnascenarija
AssessmentofecosystemservicesfortheareaDravaMolveaccordingthreepotentialscenarios
O11
18
B.K.Hackenberger,O.Antoni,.Lonari
Modeliranje populacije vrste slabo istraenih karakteristika primjer popovske gaovice
(Delminichthysghetaldii)napodrujuOmble
Modeling population of a poorly investigated species example on Delminichthys ghetaldii in the
riverOmbla
th
12.HBKxi12 CBC
19
O12
K.ganec,F.Antonelli,I.PopoviDujmovi,N.Jeran
Upotrazizaouvanimrijekama:identifikacijanajznaajnijihrijekazazatituprirodeuHrvatskoj
Lookingforpristinerivers:identificationofthemostoutstandingriversofhighconservationvalueinCroatia
O13
20
A.Dupli,D.Jeli,B.Bari,B.JanevHutinec,P.Kutlea,I.Cizelj
ProtokolprocjenerizikaodinvazivnostistranihvrstakornjaauHrvatskoj
ProtocolfortheinvasivenessriskofthealienturtlespeciesinCroatia
O14
21
D.Matoevi,I.Frani,I.Luki
Kakoseboritiprotivinvazivnevrste?Biolokosuzbijanjekestenoveoseikariceunesenomvrstom
parazitoida
How to control invasive species? Classical biological control of Dryocosmus kuriphilus with
introducedparasitoidspecies
O15
22
B.Glamuzina
teteikoristiinvazijauneenihribauslivuNeretve
DamagesandbenefitsofintroducedfishinvasionsintheNeretvariverwatershed
O16
23
K.Jeli,J.Jeremi
Protokol za dojavu i djelovanje u sluaju pronalaska uginulih, bolesnih ili ozlijeenih strogo
zatienihmorskihivotinja
Protocolforalertingandmonitoringofdead,sickorinjuredstrictlyprotectedmarinespecies
O17
24
D.Hamidovi,V.Lindi,P.Krstini
Kadaidaliljudiiimiimogudijelitiistosklonite?
Whenandifhumansandbatsmaysharethesameroost
2.Simpozijedukacijebiologije
2ndBiologyEducationSymposium
O18
25
B.Bajd
Simplifiedidentificationkeysfororganisms
O19
26
D.Garai,I.Radanovi,.Luka,M.SertiPeri
Afinitetuenikazaizborzanimanjaupodrujubiologije
Students'affinitiesforprofessionalcarrierinthefieldofbiology
O20
27
.Luka,T.alamon,T.DragiRunjak,N.Sinkovi
Utjecajaktivnostiupoznavanjabiljakanamotivacijuiznanjeuenika
The impact of activities of familiarizing students with plants on the motivation and knowledge of
students
th
12.HBKxii12 CBC
28
O21
D.Novoseli,N.Kleteki,M.Buli
Motivacijauenikasedmihrazredazastjecanjeznanjaiznastavnogpredmetabiologija
Seventhgradersmotivationforknowledgeacquisitionofelementaryschoolbiology
O22
29
A.Mojsoviui,O.Digula
EbiologijanaZdravstvenomveleuilitu
EbiologyattheUniversityofappliedhealthsciences
O23
30
A.Belani
Uporabadigitalnihnastavnihsadrajaunastavibiologije
Theuseofdigitaleducationalcontentinteachingbiology
O24
31
L.ari,M.Varga
ProjektnaterenskanastavanaobroncimaMedvednice
ProjectfieldclassesontheslopesofMedvednica
O25
32
M.Cindri,D.Crnec,M.Mesari
AnalizastavovaiinteresauenikasrednjihkolaoprirodnimvrijednostimaMeimurskeupanije
MiddleschoolstudentsattitudesandinteresanalysisabouttheirnaturalvaluesintheMeimurje
county
O26
33
M.LabaHorvat,M.Raan
Razvoj jezinih, drutvenih i upravljakih kompetencija u uenikim istraivakim projektima u
programuGLOBEibiologiji
Development of linguistic, social and management competencies in GLOBE and biology student
researchprojects
O27
34
Z.Pongractimac,R.HalapirFrankovi
Predstavljanjerezultatapotprojekataistraivanjeirekreacijauprirodiinastavnemetodeubiologijii
kemijiuokviruprojektaafirmativnanastavaiinovativnouenjeipouavanjeugimnazijamauokviru
hrvatskogkvalifikacijskogokviraIPAQPeta
Presenting the results of subprojects research and recreation in nature and teaching methods in
biology and chemistry in the framework of affirmative and innovative learning and teaching in
gymnasiumswithinthecroatianqualificationsframeworkIPAQPeta
O28
36
.Kadi,J.Pavlic
PrimjerintegriranjarazliitihnastavnihpredmetanatemuzatitelokalitetaBedekovievegrabe
An example of integrating different school subjects focusing on protecting the site Bedekovieve
grabe
O29
37
N.Graovac,E.Merdi
Istraivanjestavovauenikaoprehrani
Pupil'sdietrelatedattitudestohealthylifestyle
th
12.HBKxiii12 CBC
38
O30
A.pernjak
Anatomyandphysiologyofhumanbodyteachingwithnaturalmaterials
O31
38
M.Rui,I.Podrug,D.Sirovina
Istraivanjeuenikograzumijevanjaosnovnogbiolokogkonceptamenstruacijskogciklusa
Resarchofstudentunderstandingofbasicbiologicalconceptofthemenstrualcycle
O32
39
D.Crnec
Analizazastupljenostirazliitihtipovazadatakainjihovihkognitivnihrazinautestovimaupanijskih
natjecanjabiologijezaosmerazredeod2009.2014.godine
Analysis of the representation of different types of tasks and their cognitive levels in countylevel
competitiontestsinbiologyforeighthgradesfrom2009to2014
40
O33
S.Raan,M.Raan
Procjenakvalitetepitanjaizprirodeidrutvaza4.razredO
Estimatingthequalityoftestquestionsfromnatureandsocietyinthefourthgrade
O34
42
V.Begi,M.Basti,I.Radanovi
Utjecajkvaliteteznanjauenikanarjeavanjezadatakaviihkognitivnihrazina
Influenceofstudentsknowledgeonperformanceinsolvingcomplexcognitivetasks
O35
43
L.Lugar,A.Musta
Uspjenostuenika8.razredaurjeavanjupisanihzadatakaizbiologije
Efficiencyofthe8thgradepupilsinsolvingwrittenassignmentsinbiology
Evolucija,sistematika,filogenijaibiogeografija
Evolution,systematics,phylogenyandbiogeography
O36
44
I.Buj,Z.Mari,M.aleta,T.Mihinja,R.anda,D.Zanella,P.Mustafi,M.Mrakovi
EvolucijauDinaridimaanalizafilogeografskeipopulacijskegenetskestruktureribarodaDelminichthys
Evolution in Dinarids integrative analysis of phylogeography and population genetic structure of
thefishgenusDelminichthys
O37
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M.Jeli,G.Klobuar,F.Grandjean,J.Amouret,D.Franjevi,M.Futo,D.Ghia,G.Fea,M.Govedi,
M.Vuci,N.Puillandre,I.Maguire
Filogenija i filogeografija bjelonogog raka (Austropotamobius pallipes kompleks) u rijekama
jadranskogbazena
Phylogenyandphylogeographyofthewhiteclawedcrayfish(Austropotamobiuspallipescomplex)in
theAdriaticbasinrivers
O38
46
F.Rebrina,J.Skejo,N.Tvrtkovi
Prvi rezultati inventarizacije vrsta ohara, bogomoljki i ravnokrilaca (Insecta: Polyneoptera) ireg
th
12.HBKxiv12 CBC
podrujaplanineDinare
FirstresultsofinventarisationofBlattodea,MantodeaandOrthoptera(Insecta:Polyneoptera)ofthe
Dinaramountainarea
47
O39
L.Lovreni,J.Lajtner,M.Podnar
MolekularnofilogenetikaifilogeografskaanalizapopulacijavrsteHolandrianaholandrii(C.Pfeiffer,
1828)(Mollusca:Gastropoda)uHrvatskoj
Molecular phylogenetic and phylogeographic analysis of populations of Holandriana holandrii (C.
Pfeiffer,1828)(Mollusca:Gastropoda)inCroatia
O40
48
M.rut,A.tambuk,A.Erdelez,D.Polovi,M.Furdek,M.Peharda,G.Klobuar
UtjecajobalnogoneienjanaepigenetikuireproduktivnifitnesvolkaHexaplextrunculus
Impact of coastal pollution on epigenetics and reproductive fitness of marine snail Hexaplex
trunculus
O41
49
A.tambuk,S.Dennis,D.Polovi,M.rut,V.SoriaCarrasco,Z.Gompert,V.Bakovi,G.Klobuar,
P.Nosil
Brzaevolucijadagnjepodutjecajemoneienjaeksperimentalnagenomika
ExperimentalgenomictestsforpollutiondrivenrapidevolutionintheMediterraneanmussel
O42
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D.Polovi,S.Dennis,A.tambuk,M.rut,V.SoriaCarrasco,Z.Gompert,V.Bakovi,G.Klobuar,
P.Nosil
GenetikaarhitekturamorfolokihobiljejadagnjeMytilusgalloprovincialis
GeneticarchitectureofmorphologicaltraitsinthemediterraneanmusselMytilusgalloprovincialis
O43
51
N.Jantol,T.Keki
AnalizaraznolikostikloroplastneDNAifilogeografijaljekovitekadulje(SalviaofficinalisL.)
AnalysisofchloroplastDNAdiversityandphylogeographyofcommonsage(SalviaofficinalisL.)
O44
52
I.Reetnik,D.Barievi,D.BatrRusu,K.CaroviStanko,P.Chatzopoulou,Z.DajiStevanovi,M.
Gonceariuc,M.Grdia,D.Gregura,A.Ibraliu,M.JugDujakovi,E.Krasniqi,Z.Liber,S.Murti,D.
Peanac,I.Radosavljevi,Gj.Stefkov,D.Steevi,I.otari,K.Varbanova,Z.atovi
Genetska raznolikost prirodnih i kultiviranih populacija dalmatinske kadulje (Salvia officinalis L.,
Lamiaceae)
Patternsofgeneticdiversityinwildandcultivatedpopulationsofdalmatiansage(SalviaofficinalisL.,
Lamiaceae)
O45
54
I.Radosavljevi,Z.atovi,Z.Liber
Klonalna struktura endemine i ugroene kratkozupaste kadulje Salvia brachyodon (Lamiaceae)
utvrenamikrosatelitnimbiljezima:znaenjezaupravljanjeizatitu
Microsatellite evidence for high clonality in narrow endemic and endangered Salvia brachyodon
(Lamiaceae):implicationsformanagmentandconservation
O46
55
J.Mlinarec,D.Franjevi,L.Bokor,V.Besendorfer
th
12.HBKxv12 CBC
Evolucija,raznolikostifilogenija5SrDNAjedinicaponavljanjaudiploidnimipoliploidnimvrstamaiz
tribusaAnemoninae(Ranunculaceae)
Evolution,diversityandphylogenyof5SrDNAunitarraysindiploidandpolyploidmembersofthe
tribeAnemoninae(Ranunculaceae)
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O47
N.Koleti,M.Novosel,N.Kevi,D.Franjevi
Evolucijamahovnjakarekolonizacijomslatkovodnihekosustava
Evolutionofbryozoansviarecolonisationoffreshwaterecosystems
O48
57
M.Djurakic,A.Herrel
Room to maneuver: physical environment constraints the pattern and rate of carapace shape
evolutioninChelonians
O49
58
M.Djurakic,V.Milankov
A recognition of species taxon is a tail of microevolutionary processes in Testudo marginata: an
evidenceofasexspecificenvironmentallyinducedvariationofplastronmorphology
1.BalkanskiherpetolokisimpozijusuorganizacijisHrvatskimherpetolokimdrutvom
1stBalkanHerpetologicalSymposiumincoorganizationwithCroatianHerpetologicalSociety
IO1
59
M.Podnar,I.Grbac,N.Tvrtkovi,B.BruvoMaari,W.Mayer
Komparativnafilogeografijatrivrstezapadnobalkanskihguterica(Reptilia,Lacertidae)preklapajuihareala
Comparative phylogeography of the three widely codistributed endemic Western Balkans lacertid
lizards(Reptilia,Lacertidae)
IO2
60
M.Luka
Zarazne bolesti vodozemaca to se ve zna, to je novo, a to treba imati na umu kako bi se
sprijeilonjihovoizumiranje
Emergingamphibiandiseaseswhatisknown,whatisnewandwhatshouldbeborneinmindto
preventtheirextinction
O50
61
E.PadoaSchioppa,M.Polo
Distribution,abundanceanddetectionprobabilityofLacertidaeinfourCroatianislands
O51
62
S.Bakiera,D.Jeli
Uestalostivorodneguterice,ZootocaviviparaLichtenstein1823napodrujuzapadnogBalkana
Howcommonisthecommonlizard,ZootocaviviparaLichtenstein1823inWestBalkanarea?
O52
63
A.Zimi
Distribution (horizontal and vertical) and conservation status of herpetofauna of Sarajevo Canton
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th
12.HBKxvi12 CBC
63
O53
E.Kleteki,S.Leiner,M.Podnar
Rezultatiprvegodinesustavnihistraivanjasastava,distribucijeibrojnostiherpetofauneNP"Plitvikajezera"
Composition,distribution,andabundanceofamphibiansandreptilesofthePlitviceLakesNational
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O54
64
K.vanLieshout,S.vanStraten,M.J.A.Weterings,I.M.Dmmer,D.Jeli
Ecological limitations and past events affect species distribution: assessing the quality of spatial
distributionmodelsfortworeptilespecies
O55
65
K.vanLieshout,S.vanStraten,M.J.A.Weterings,I.M.Dmmer,D.Jeli
Preliminarydataontheassessmentoftheeffectivenessofprotectedareasfortworeptilespecies
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O56
65
M.Vujanovi,D.Jeli
Procjena osjetljivosti faune vodozemaca i gmazova u Hrvatskoj i Bosni i Hercegovini uz definiranje
vanihherpetolokihpodruja
Sensitivity assessment of fauna of amphibians and reptiles of Croatia and Bosnia and Herzegovina
withdetectionofimportantherpetologicalareas
O57
66
K.Ljubisavljevi,L.Polovi,V.Ikovi,S.Vuksanovi,T.D.Vukov
Habitat requirements and abundance of Dinarolacerta mosorensis and D. montenegrina in the
mountainsofMontenegro
O58
67
M.Zadravec,R.Cesarec,Z.Tadi
BiologijaposkokaukamenolomuBizek
BiologyofthenosehornedviperintheBizekquarry
O59
68
A.uri,A.Zimi,D.Jeli
NewdataanddistributionofcommonspadefoottoadPelobatesfuscus(Laurenti,1768)inWesternBalkans
O60
69
K.avlovi,D.Karaica,I.Buj,V.MietiStankovi,L.Choleva,D.Jeli
Komparativna morfologija, ekologija i analiza strukture hrvatske populacije Pelophylax esculentus
kompleksa
Comparative morphology, ecology and structure analysis of the Pelophylax esculentus complex
populationinCroatia
O61
71
M.Jeli,M.Vuci,G.Klobuar,P.Korlevi,D.iki,D.Franjevi,D.Jeli
Molekularnaistraivanjazelenihaba(rodPelophylax)uHrvatskojpreliminarnirezultati
Molecularstudyonwaterfrogs(genusPelophylax)inCroatiapreliminaryresults
O62
72
T.Voloen,B.Prakljai,D.Karaica,D.Jeli
th
12.HBKxvii12 CBC
Procjena starosti jedinki zelenih aba roda Pelophylax (Fitzinger, 1843) iz populacije sa Skadarskog
jezera,CrnaGora
Age estimation of green frog individuals belonging to the Pelophylax genus (Fitzinger, 1843) from
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72
O63
M.Vuci,M.Jeli,B.Prakljai,G.Klobuar,D.Jeli
Odreivanjevrstaihibridazelenihaba(rodPelophylax)saSkadarskogjezera(CrnaGora)analizom
molekularnihbiljega
Determination of species and hybrids of water frogs (genus Pelophylax) from the Skadar Lake
(Montenegro)usingmolecularmarkers
O64
74
Lj.Tomovi,A.Uroevi,T.D.Vukov,R.Ajti,K.Ljubisavljevi,I.Krizmani,D.Jovi,N.Labus,S.
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Threatening levels and extinction risks based on distributional, ecological and lifehistory datasets
(DELH)versusIUCNcriteriaexampleofSerbianreptiles
O65
74
V.Vergilov,N.Tzankov,B.Zlatkov
Hemipenialmorphologyasausefulcomplementarytoolsupportingthefullspeciesstatusofclosely
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O66
75
I.Cizelj,M.Luka,D.Jeli,I.Perani
Exsitu dranje autohtonih vodozemaca i gmazova u Zoolokom vrtu grada Zagreba u svrhu
edukacije,zatiteiistraivanja
Exsitu keeping of native amphibians and reptiles in Zagreb Zoo for education, conservation and
researchpurposes
O67
76
A.Golubovi
IwasaNinjaatyouragesaidanelderlytortoisetoahatchlingontogenyshiftinagility
O68
77
E.unje,D.Jeli,M.Mller,R.krijelj,V.Helfer
Comparative morphology of Salamandra atra prenjensis (Bosnia and Herzegovina) with its
nominotypicsubspeciesSalamandraatraatra(Austria)
O69
77
A.Ivanovi,J.W.Arntzen
Application of micro CT scanning and 3D geometric morphometrics in exploring evolution of skull
shapeinTriturusnewts(Caudata:Salamandridae)
O70
78
E.Sadanaku,I.Haxhiu
DataaboutStripedneckedTerrapin,MauremysrivulatainVloraBay,Albania
O71
78
C.Samoila,D.Cogalniceanu,I.Ruben,M.Bogdan,A.Dumitrescu
ModelingthepotentialdistributionoftheSpurthighedtortoise(Testudograeca)andtheHermanns
tortoise(T.hermanni)atthenorthernlimitoftheirrangeinRomania
th
12.HBKxviii12 CBC
79
O72
D.Stankovi,.Goriki,M.NpruAljani,M.Pavievi,M.Kuntner,A.Snoj,G.Aljani
DetectionofProteuseDNAinkarstgroundwater
O73
80
A.tih,I.Buri
Znaajkepopulacijerijenekornjae,MauremysrivulataizKonavoskogpolja
CharacteristicsoftheBalkanTerrapinpopulationfromKonavlefield
Biologijamora
MarineBiology
O74
81
J.Duli,B.Dragievi
TrenutnistatusbioraznolikostiihtiofauneJadranskogaiSredozemnogamora
ThecurrentstatusoftheAdriaticandMediterraneanfishbiodiversity
O75
82
P.Lui,J.Njire,P.Krui,B.Pestori,D.Drakulovi
MikrozooplanktonBokokotorskogzaljevaiobalnogpodrujajunogJadrana
MicrozooplanktonintheBokaKotorskaBayandinthecoastalregionofthesouthAdriaticSea
O76
83
S.Bosak,M.Mejdandi,I.Bonjak,J.Godrijan,Z.Ljubei
TaksonomijaiprostornaraspodjelafitoplanktonaujunomJadranu;zimskiaspekt
TaxonomyandspatialdistributionofphytoplanktoninthesouthAdriatic;winteraspect
O77
84
A.uljevi,B.Antoli
UsklaenorazmnoavanjealgirodaUlva
SynchronizedreproductionofUlvaspecies
O78
85
D.Lui,I.Onofri,M.Miloslavi,B.Gangai,I.Ivani
NovonastalepromjenefaunehidromeduzasjevernogJadrana
RecentchangesinthenorthernAdriatichydromedusanfauna
O79
86
M.Miloslavi,D.Lui
Vremenska dinamika zajednica kalanoidnih kopepoda u izoliranom morskom jezeru (Veliko jezero,
juniJadran):vezesklimatskimpromjenamanaveojskali
Temporal patterns of the calanoid copepod community in Veliko jezero, an isolated marine lake
(southAdriaticSea):linkstoalargerscaleclimatechanges
87
O80
G.Plesli,G.Kett,N.RakoGospi,T.Vuur,D.Holcer,J.MioiStoi,P.Mackelworth
Utjecaj strategije hranjenja na drutvenu strukturu dobrih dupina (Tursiops truncatus) u istonom
Jadranu,Hrvatska
Influence of foraging strategies on social structure of bottlenose dolphins (Tursiops truncatus) in
easternAdriaticSea,Croatia
th
12.HBKxix12 CBC
88
O81
A.Jaklin,D.PaviiHamer,A.Barievi,W.E.G.Muller,R.Batel,B.Hamer
PrilogpoznavanjuekologijespuveSuberitesdomuncula(Olivi,1872):prekomorfologijedoanalize
populacije
Contribution to the ecology of the sponge Suberites domuncula (Olivi, 1872): from morphology to
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O82
89
I.Cvitkovi,M.Despalatovi,A.uljevi,S.Roi,B.Antoli
EkolokistatuslivadamorskecvjetnicePosidoniaoceanicauzduhrvatskeobale
EcologicalstatusofPosidoniaoceanicameadowsalongtheCroatiancoast
O83
90
M.Bilan,G.Dionsio,R.Calado,R.Rosa
Ranirazvojnistadijidvatropskapua(Opisthobranchia)uklimatskimpromjenama
OntogenicdevelopmentoftwotropicalOpisthobranchmollusksinachangingocean
O84
91
A.Jaklin,F.Marin,D.Medakovi
MorfologijaistrukturakuicepuevastranjokrnjakaAplysiaspp.
MorphologyandstructureoftheopisthobranchgastropodshellAplysiaspp.
O85
92
R.Graan,T.Polak,G.Lackovi,B.Lazar
ivotnicikluspsamekua(Musteluspunctulatus)usjevernomisrednjemJadranskommoru
Life history traits of a Blackspotted smoothhound (Mustelus punctulatus) in the northern and
centralAdriaticSea
O86
93
P.Krui,M.Kvarantan,S.akor,V.Loni,S.Motah,M.Andri
NaseljakoraljanakoraligenupuinskihpliinauistonomdijeluJadranskogmora
ThecoralassemblagesofanoffshorecoralligenebanksintheeasternAdriaticSea
O87
94
I.Bonjak,H.Mihanovi,I.Cetini,P.Krui,S.Bosak,M.Mejdandi,Z.Ljubei
Prostornaraspodjelabiooptikihitermohalinihsvojstavausjeverozapadnomdijelujunojadranske
kotline
SpatialdistributionofbioopticalandthermohalinecharacteristicsinNWpartofsouthernAdraticpit
O88
96
P.Krui
KoraljipodmorskihpiljaJadranskogmora
CoralfaunaofsubmergedmarinecavesintheAdriaticSea
6.SimpozijHrvatskogDrutvazabiljnubiologiju
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6 SymposiumoftheCroatianSocietyofPlantBiologists
O89
97
N.Bauer,M.Jurani,M.Moibob,G.Razdorov,D.LeljakLevani
Protein TaMAB2 sudjeluje u remodeliranju kromatina i metabolizmu RNA u zigoti i 2staninom
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th
12.HBKxx12 CBC
Wheat MATHBTB protein TaMAB2 participates in chromatin remodelling and RNA metabolism in
zygoteand2celledproembryo
98
O90
J.AntunoviDuni,Lj.Mihaljevi,D.imi,A.Lali,J.Kovaevi,V.Cesar
Oporavakklijanacajemarehidracijomnakonstresaizazvanogsuom
Recoveryofdroughtstressedbarleyseedlingsbyrewatering
O91
99
S.Mlinari,V.Cesar,H.Lepedu
Fotosintetskiodgovormladihirazvijenihlistovasmokvenavisokiintenzitetsvjetlosti
Photosyntheticresponseofyoungandmaturefigleavestohighirradiation
O92
100
L.Begovi,D.Pavokovi,S.Mlinari,A.Lali,V.Cesar
Odreivanje izoformi peroksidaza skupine III i lakaza tijekom razvoja stabljike jema (Hordeum
vulgareL.)
DeterminationofclassIIIperoxidaseandlaccaseisoformsduringdevelopmentofbarley(Hordeum
vulgareL.)stem
O93
101
I.ola,D.Poljuha,J.Bili,S.Duda,T.Bilui,J.Marki,G.Rusak
Usporedbagastrointestinalnestabilnostihrvatskihdivljihjestivihbiljaka:analizafenolnihspojeva
ComparisonofgastrointestinalstabilitybetweenCroatianwildedibleplants:caseofphenolics
O94
103
P.Peharectefani,K.Perica,K.Landeka,D.BarZvi,B.Balen
ASRsliniproteiniotkriveniuinvitrokulturikaktusaMammillariagracilis
ASRlikeproteinsdetectedininvitrogrowncactusMammillariagracilis
3.SimpozijHrvatskogentomolokogdrutva
3rdSymposiumoftheCroatianEntomologySociety
O95
104
T.Bogdanovi,M.Grgi
Vretenca(Odonata)otokaKrkaugroenostistatus
Dragonflies(Odonata)oftheKrkIslandendangermentandstatus
O96
105
I. Kurtek, M. Sudari Bogojevi, N. Turi, G. Vignjevi, I. Vruina, . Zahirovi, M. Bistrovi, E.
Merdi
SezonskadinamikakomaracanapodrujuupanjskePosavinenakonpoplave2014.godine
SeasonaldynamicsofmosquitoesontheterritoryofPosavinanearupanjaafterthe2014flood
106
O97
E.Raspudi,M.Brme,A.Budimir,Z.Plea,J.Zdelian
Epitrixhirtipennis(Melsheimer,1847)duhanovbuha,novilanentomofauneHrvatske
Epitrixhirtipennis(Melsheimer,1847)tobaccofleabeetlenewmemberofCroatianentomofauna
O98
107
T.Koren,S.Gomboc
th
12.HBKxxi12 CBC
RaznolikostnonihleptiraStrahinjiceiIvanice
DiversityofmothfaunaofStrahinjicaandIvanica
108
O99
M.ag,N.Turi,.Zahirovi,B.Lau,T.Koren,M.Zadravec
Strukturasaproksilnihkornjaa(Coleoptera)nizinskihuma
Saproxylicbeetle(Coleoptera)assemblagesinlowlandforest
O100
109
I.Vruina,.Zahirovi,G.Vignjevi,E.Merdi,A.Klobuar
VektorAedesalbopictusAzijskitigrastikomaracuOsijeku,Hrvatska
VectorAedesalbopictusAsiantigermosquitoinOsijek,Croatia
Biologijakopnenihvodaikopna
Biologyoffreshwaterandterrestrialecosystems
IO3
110
M.Sabovljevi,A.Sabovljevi
Bryophyte conservation biology in Europe insights into contemporary achievments, problems,
solutionsandmanagement
O101
111
M.Piria,T.Tomljanovi,N.prem,D.Matuli,I.Anii,R.Safner,T.Treer
PromjenesastavaihtiofaunerijekeSaveuHrvatskojtijekomposljednjegdesetljea(2003.2013.)
IchthyofaunalchangesoftheRiverSava,Croatiaoverlastdecade(20032013)
O102
112
M.Milia,D.iki,I.oli,D.Grozi,T.Mandi
Elektrinapoljaurbanipritisaknaorganizmeukopnenimvodama
Electricfieldstheurbanpressureonfreshwaterorganisms
O103
113
R.MatonikinKepija,M.Milia,M.Ivkovi,Z.Mihaljevi
UtjecajhidrolokogstresanaobratajuNPKrka
InfluenceofhydrologicalstressonperiphytoninKrkaNationalpark
O104
114
M.Ivkovi,M.Milia,V.Baranov,Z.Mihaljevi
Zakrivenostvegetacijomkaoglavnipokretastrukturezajedniceizvorskihdvokrilaca
CanopycoverageasamaindriverinstructuringspringDipteraassemblages
O105
115
J.Lajtner,I.Zrinak,M.poljar,T.Draina,I.Radanovi,B.rgar,A.Ostoji
Raznolikost stanita litoralne zone kao odrednica sastava zajednice pueva u plitkom eutrofnom
jezeru
Habitatsheterogeneityofthelittoralzoneinthedeterminationofsnailassemblagesintheshallow
eutrophiclake
O106
116
L.Vidati,M.Ljutina,I.Ivanek,D.Lisii
Istraivakaaktivnostdinarskogvoluhara(DinaromysbogdanoviMartino,1922)
th
12.HBKxxii12 CBC
ExploratoryactivityofMartino'ssnowvole(DinaromysbogdanoviMartino,1922)
117
O107
M.Kova,M.Ljutina,D.Hamidovi,M.Brai,D.Lisii
Prvipodacioakustinomrepertoarudinarskogvoluhara(Dinaromysbogdanovi)uzatoenitvu
FirstdataontheacousticrepertoireofBalkansnowvole(Dinaromysbogdanovi)incaptivity
Toksikologijaiekotoksikologija
Toxicologyandecotoxicology
118
IO4
B.K.Hackenberger
Kvalitativnipristupekotoksikolokimistraivanjimaformalnapotrebailinunost
Thequalitativeapproachinecotoxicologicalstudiesaformalneedoranecessity
O108
119
O.Jovanovi,B.K.Hackenberger,.Lonari,M.Velki,G.Palijan
Utjecajpesticidanaabljajaja
Effectsofpesticidesonfrogs'eggs
O109
120
S.Hayretda,B.Erkmen,E.Tac,F.Erko
Spermiotoxicity and embryotoxicity of esbiothrin in sea urchin Paracentrotus lividus using short
termmarinetoxicitylarvaldevelopmentmethods
O110
121
A.Oroli,D.K.Hackenberger,.Lonari,V.Feigl,B.K.Hackenberger
Dinamikarastaalginamedijimasdodatkomcrvenogmulja
Algaegrowthdynamicsonmediumsuplementedwithredmud
O111
122
J.P.Bourdineaud,M.rut,A.tambuk,M.Tkalec,D.Brthes,K.Malari,G.Klobuar
Electromagnetic fields at the mobile phone frequency (900 MHz) trigger the onset of the general
stressresponsealongwithDNAmodificationsinEiseniafetidaearthworms
O112
122
B.Hamer,A.Barievi,N.Medi,V.S.Perusco
AnalizaPAHmetabolitauuiodabranihkomercijalnihribaduistoneJadranskeobale
BilePAHmetabolitescontentinseveralcommercialfishesalongeasternAdriaticcoast
O113
123
Lj.Prester,J.Macan
Alergenigrinja,oharaiplijesniusedimentiranojprainiukuanstvuuHrvatskoj
Allergensofmites,cockroachesandmouldsinsettleddustinhousholdsinCroatia
O114
124
J.Krasi,D.iki
Sinergistikiuincivazodilatacijskihiantikoagulacijskihlijekovanaoksidativnistresumozgu
Synergisticeffectsofvasodilatationalandanticoagulativedrugsonbrainoxidativestress
th
12.HBKxxiii12 CBC
Mikrobiologija
Microbiology
126
O115
M.erugaMusi,A.Slovi,M.Pinteri
Komparativnaanalizagenomafitoplazmi:odnestabilnostigenomadoprilagodbenadomaine
Comparativeanalysisofphytoplasmagenomes:fromgenomeinstabilitytohostadaptation
O116
127
D.kori,M.erugaMusi,S.erni
Molekularnatipizacijauzronikafilodijeuljanerepice
Moleculartypingindecipheringetiologyofrapephyllodydisease
O117
128
V.Karin,M.Jei,M.Idojti,M.urkoviPerica
RaznolikostgljiveCryphonectriaparasiticauzarastajuimkalusimakestena
DiversityofCryphonectriaparasiticainhealedchestnutcankers
O118
129
L.Nuskern,Z.Katani,Lj.Krstin,M.Jei,M.urkoviPerica
Opstojnost virusa Cryphonectria hypovirus 1 moe ovisiti o njegovom prijenosu izmeu razliitih
tipovavegetativnekompatibilnostigljiveCryphonectriaparasitica
Persistence of Cryphonectria hypovirus 1 in Cryphonectria parasitica might be affected by the
transferbetweenthedifferentvegetativecompatibilitytypesofthehost
Komparativnafiziologija,imunobiologijaibiologijaovjeka
Comparativephysiology,immunobiologyandhumanbiology
IO5
130
R.Karli,P.Polak,A.Koren,R.Thurman,R.Sandstrom,M.S.Lawrence,A.Reynolds,E.Rynes,K.
Vlahoviek,J.A.Stamatoyannopoulos,S.R.Sunyaev
Predvianjeishodinihstanicarakanatemeljuorganizacijekromatina
Usingchromatinorganizationtopredictcancercelloforigin
O119
132
V.Tadi,P.BaiPalkovi,B.Sasi,M.Klasi,P.Kora
ReplikacijauBstaninimlimfomima
ReplicationinBcelllymphoma
O120
133
V.Vukovi,M.Acman,M.Klasi,P.Dobrini,P.Kora
RazvojtestovazaanalizumetilacijeDNAregulatornihregijagenaGMNNiEZH2injihovaprimjenau
istraivanjupodtipovadifuznogBvelikostaninogalimfoma
Assay development for DNA methylation analysis of regulatory regions of genes GMNN and EZH2
andtheirapplicationinevaluationofdiffuselargeBcelllymphomasubtypes
O121
134
R.unec
HistokompatibilnostiimunogenetikautransplantacijisolidnihorganauHrvatskoj
HistocompatibilityandimmunogeneticsinsolidorgantransplantationinCroatia
th
12.HBKxxiv12 CBC
135
O122
Z.Grubi
Histokompatibilnost i imunogenetika u alogenoj transplantaciji krvotvornih matinih stanica u
Hrvatskoj
Histocompatibility and immunogenetics in allogeneic hematopoietic stem cell transplantation in
Croatia
O123
136
H.Lepedu,A.Kurtovi,I.Jozi,D.GaoSoka,I.Marinko,V.Cesar
Akutniutjecajkombiniranogtretmanadijafragmalnogdisanjaiprogresivnemiinerelaksacije
na antioksidacijsku uinkovitost sline u mladih odraslih ljudi povezan s njihovim crtama
osobnosti
Acuteresponsesofsalivaantioxidantcapacitytocombineddiaphragmaticbreathingandprogressive
musclerelaxationtreatmentsinyoungadultsrelatedtotheirpersonalitytraits
O124
137
V.lipogor,T.GunholddeOliveira,J.J.M.Massen,Z.Tadi,T.Bugnyar
Monkeys with personality: consistent interindividual differences in common marmosets
(Callithrixjacchus)
O125
138
L.Vrankovi,I.Dela,S.Relji,.Huber,J.Kusak,K.Klobuar,G.Krivi,D.Octenjak,N.Mikinac,Z.
Stojevi,J.Aladrovi
Usporedba masnokiselinskog sastava potkonog masnog tkiva smeeg medvjeda (Ursus arctos) i
sivogvuka(Canislupus)
Comparisonoffattyacidcompositionofsubcutaneousadiposetissueofbrownbear(Ursusarctos)
andgraywolf(Canislupus)
POSTERSKAPRIOPENJA/POSTERPRESENTATIONS
Konzervacijskabiologija,zatitaprirodeiokolia
Conservationalbiology,natureandenvironmentprotection
P1
141
I.Bori,V.PosavecVukeli,T.Kirin
Kockavica(FritillariameleagrisL.,Liliaceae)10godinaakcijepraenjastanjazaosnovneisrednje
kole
Snake's head fritillary (Fritillaria meleagris L., Liliaceae) 10 years of monitoring campaign for
primaryandsecondaryschools
P2
142
A.ani,Z.Mesi,M.kunca,A.Popija,E.Patev,S.Kapelj,J.Krian
Definiranje ekolokih ciljeva u skladu s ekolokom mreom Natura 2000 za potrebe revitalizacije
Erdutskodaljskogdunavca
Defining ecological objectives according to ecological network Natura 2000 for the purpose of
revitalizationofErdutDaljdunavac
P3
143
M.Gubi,M.Barievi,D.Fantina,A.Mojsoviui
SuivotljudiiprirodenaprimjeruNacionalnogparkaPlitvikajezerajeliistomogue?
th
12.HBKxxv12 CBC
CoexistenceoflocalpopulationandnatureintheexampleofthePlitviceLakesNationalParkisit
possible?
144
P4
G.Guvica,I.Bonjak,A.Bielen,D.Babi,B.RadanoviGuvica,L.ver
Analizametodazapraenjeprijelazadivljihivotinjaprekozelenihmostova
Analysisofmethodsforwildlifemonitoringongreenbridges
P5
145
G.Guvica,I.Gabri,K.Protrka,Z.Grguri,L.ver
PreliminarnirezultatiistraivanjavukovauParkuprirodeBiokovo
TheresearchofwolvesintheNatureParkBiokovopreliminaryresults
P6
146
S.Hudina,M.Samardi,A.Luci,M.Jeli,I.Maguire
Nova strana vrsta deseterononih rakova (mramorni rak (Procambarus fallax (Hagen, 1870) f.
virginalis)uslatkovodnimekosustavimaHrvatske
Newnonindigenouscrayfish(themarblecrayfish(Procambarusfallax(Hagen,1870)f.virginalis)in
Croatianfreshwaters
P7
147
J.Kusak,.Huber,N.Trenc,J.Jeremi,S.Desnica
Smjernicezaprocjenuutjecajavjetroelektrananavelikezvijeri
Guidelinesforassessmentofwindfarmsimpactonlargecarnivores
P8
148
.Lonari,M.Jambrovi,B.K.Hackenberger
ModificiraniLeslieLefkovitchgenetikialgoritamskimodelskisustavdinamikepopulacijakomaraca
ModifiedLeslieLefkovitchgeneticalgorithmhybridmodelssystemofmosquitopopulationdynamic
P9
149
M.Mazija,Z.Domazetovi,B.Benina,A.tefan,.Drdar
imiinaBrijunimarezidencijalnaelitailimasovniturizam?
BatsonBrijuniresidentialeliteormasstourism?
P10
150
A.Mikuka,A.Felja,D.Bjedov,K.Kajan,M.Blaevi,A.Zovko,P.Beni
Rasprostranjenostitrendgnijezdeepopulacijevranegaac(CorvusfrugilegusL.1758)napodruju
Osijeka
Distributionandtrendofbreedingpopulationofrooks(CorvusfrugilegusL.1758)inOsijek
P11
151
A.Mikuka,L.Begovi,I.Mihi,K.Kljaji,R.Grgac
Optimizacijametodazamolekularnoodreivanjespolakodaplji(Ardeidae)
Optimizationofmethodsformolecularsextypinginherons(Ardeidae)
P12
152
S.NovakAgbaba,I.Frani
Biolokazatitapitomogkestenagljivamaantagonistima
Biologicalcontrolofsweetchestnutwithantagonisticfungi
P13
153
N.Pavlus,M.Draki,D.valjek,M.Peri,D.egvi,M.Vukovi,B.Matoi,S.Blaevi
th
12.HBKxxvi12 CBC
SolarniparkKosoreutjecajinaokoliiekolokumreu
SolarparkKosoreenvironmentalimpactandNatura2000network
154
P14
N.Pavlus,M.Peri,M.Draki,M.Vukovi,S.Blaevi,.Varga,A.Ptiek,H.izmek
SidriteuuvaliMezuporat,otokBievokakosauvatiekolokumreu?
TheanchorageintheMezuporatbay,islandofBievohowtopreserveNatura2000network?
P15
155
V.Peri,A.Koi,J.Horvati
Kvalitetavodeuvodotocimapoljoprivrednog,movarnogiumskogporjeja
Waterqualityofwatercoursesdrainingagricultural,wetlandandforestedcatchments
P16
156
I.Plavac,A.Dupli,K.Jeli,J.Radovi,R.Topi,I.Ilija
EkolokamreaNatura2000tosepromijenilonakonbiogeografskogseminara?
Natura2000networkwhathaschangedafterthebiogeographicalseminar?
P17
157
I.Saboli,V.Dori,A.Dobrovi,A.Orlovi,P.Crnan,M.Rezo
IstraivanjerijetkihiugroenihsvojtimalakofauneIstre
ResearchofrareandendangeredtaxaofIstrianmalacofaune
P18
158
M.Saboli,I.Buj,M.Mrakovi,M.aleta
Strukturaivijabilnostpopulacijesrne,CapreoluscapreolusukontinentalnojHrvatskoj
StructureandviabilityoftheRoedeerCapreoluscapreoluspopulationinthecontinentalCroatia
P19
159
M.Saboli,T.ukovi,A.Dupli,P.Lunko,D.Schneider
Ocjena programa radova odravanja u podruju zatite od tetnog djelovanja voda u Hrvatskoj
uvjetiimjerezatiteprirode:temelji,izazoviiplanovizabudunost
Assessmentoftheprogrammeformaintenanceworksforprotectionagainstharmfuleffectsofwater
in Croatia nature protection conditions and measures: fundamentals, challenges and plans for the
future
P20
160
R.Skukan,N.Koleti,L.Dereanin,N.Maleevi,R.pani,S.Dekani
Drveneobaloutvrde:ekolokaalternativakamenuibetonunaobalamavodotoka
Treerevetments:ecologicalalternativetostoneandconcreteattheriverbank
2.Simpozijedukacijebiologije
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2 Biologyeducationsymposium
161
P21
S.Fabac,V.Fabijani
Primjenaonlineteajazauenjebiologijeusrednjojkoli
Applicationofonlineseminarforstudyingofbiologyinhighschool
P22
162
N.Kleteki,D.Novoseli
th
12.HBKxxvii12 CBC
Usvojenostosnovnihkoncepataizbotanikekoduenikapetograzredaosnovnekole
Acquisitionlevelofbasicbotanyconceptsbyelementaryschoolstudentsinthefifthgrade
163
P23
K.Latin,E.Merdi,M.Nit,I.Labak
Usvojenostnastavnogsadrajaprimjenomkonceptualnihmapakoduenikasrednjekole
Adoptionofteachingcontentusingconceptualmapsamongsecondaryschoolstudents
P24
164
M.Mihali,H.Kostadinovi
Utjecajkognitivnihrazinazadatakanavjerodostojnostprocjeneusvojenostikoncepatagenetikekod
gimnazijskihuenika
Impact of cognitive levels of tasks on genetics concepts assessment credibility in high school
students
P25
165
M.Raan,S.Mikuli,D.Sumpor,M.LabaHorvat
Koritenjelokalnihresursauterenskojnastavibiologije
Usinglocalresourcesinbiologyfieldwork
P26
166
J.RazlogGrlica,N.Kleteki,M.Novosel
Igrauponavljanjunastavnihsadrajavezanihuzivotnezajednicemora
Theplayinrepeatingtheeducationalcontentsaboutmarinelife
P27
167
.RomtajnBurchards,I.Bogut,.Popovi
EkoloketemeuudbenicimaRepublikeHrvatskeiSavezneRepublikeNjemake
EnvironmentalissuesintextbooksofCroatianandFederalRepublicofGermany
P28
168
M.Srko,I.tefani,R.HalapirFrankovi
UenikopoznavanjeivotinjaHrvatske
Student`sknowledgeofanimalsthatliveinCroatia
P29
169
A.ari,M.Mari
Stavoviuiteljaobiolokimnastavnimsadrajimauosnovnojkoli
Teachers'attitudestowardthebiologycurriculumcontentsinelementaryschools
Evolucija,sistematika,filogenijaibiogeografija
Evolution,systematics,phylogenyandbiogeography
P30
170
V.Bakovi,M.Schebeck,C.Stauffer,G.Ragland,H.Schuler
The potential role of ecological adaptation and Wolbachia infection in the diversification of
Rhagoletiscerasi
P31
171
N.BiliDujmui,B.K.Hackenberger
Primjenasuvremenihtehnikaprostornestatistikeuekolokimistraivanjima
th
12.HBKxxviii12 CBC
Applicationofmodernspatialstatisticaltechniquesinecologicalresearch
172
P32
M.Jeli,F.Grandjean,M.Govedi,G.Klobuar,D.Guyonnet,I.Maguire
Genetska struktura bjelonogog raka (Austropotamobius pallipes kompleks) otkriva moguu
povezanostizmeutrikrkerijeketijekomgeolokeprolosti
Genetic structure of the whiteclawed crayfish (Austropotamobius pallipes complex) revealed
possiblehistoricalconnectionsbetweenthreekarsticrivers
P33
173
L.Jovovi,B.BruvoMaari,V.MietiStankovi,M.A.Jch,M.Kuini
FilogenijaifilogeografijarodaElmis(Coleoptera,Elmidae)krkihtekuicajugoistoneEurope
Phylogeny and phylogeography of genus Elmis (Coleoptera, Elmidae) in karstic running waters in
southeasternEurope
P34
174
N.Kevi,S.RadiBrkanac,P.Peharectefani,F.Faraguna,M.Kalafati,G.Kovaevi,D.Franjevi
Simbiozahidreialge:karakterizacijaendosimbiotskihzelenihalgiizoliranihizrazliitihsojevazelenih
hidripomouelektronskemikroskopije,morfometrijskeienzimskeanalize
Hydra algae symbiosis: characterization of endosymbiotic green algae isolated from different
greenhydrastrainsusingelectronmicroscopy,morphometricandenzymeanalysis
P35
176
D.K.Hackenberger,B.K.Hackenberger,D.K.Hackenberger
ErIK:softverskaplatformazaidentifikacijugujavicaHrvatske
ErIK:asoftwareplatformforidentificationofearthwormsofCroatia
P36
177
M.Rui,M.eput
Floraotoiaoltanskogarhipelaga
Thefloraofoltaarchipelagoislets
P37
178
N.evrnja,B.Miti,D.Vladovi
AnalizadijelaherbarijaCarlaStudniczke(ord.Caprifoliaceen,LonicereeniStellateen)
TheanalysisofpartofCarlStudniczka'sherbarium(ord.Lonicereen,CaprifoliaceenandStellateen)
1.BalkanskiherpetolokisimpozijusuorganizacijisHrvatskimherpetolokimdrutvom
st
1 BalkanherpetologicalsymposiumincoorganizationwithCroatianherpetologicalsociety
P38
179
S.Bakiera,M.Vuci,D.Jeli
PopulacijskaekologijaispolnidimorfizamlivadnegutericeLacertaagilis,Linnaeus1758sDinareu
Hrvatskoj
Populationecologyandsexualdimorphisminpopulationofthesandlizard,Lacertaagilis,Linnaeus
1758onDinaramountain,Croatia
P39
180
S.Bakiera,M.Vuci,B.Lau,I.Vilaj
DoprinospoznavanjuherpetofauneDinareuHrvatskojiblieokolice
ContributiontotheknowledgeofherpetofaunaofCroatianpartofDinaramountainanditsvicinity
th
12.HBKxxix12 CBC
181
P40
M.Cipot,A.Lenik,K.Poboljaj
IspopulationofMoorfrog(Ranaarvalis)underseveredeclineinMurariverbasininSlovenia?
P41
182
A.uri,A.Zimi,D.Jeli
HerpetofaunaofPosavinaregioninBosniaandHerzegovinaandCroatia
P42
182
S.Drakuli,H.Feldhaar,D.Lisii,M.Mio,I.Cizelj,M.Seiler,T.Spatz,M.O.Rdel
Effect of environmental temperature on body condition and jumping performance of two
geographicallydistantRanatemporariapopulations
P43
183
A.Dyugmedziev,N.Tzankov,G.Popgeorgiev
Spatial replacement of close relatives, a passionate story of a mountainous population of Vipera
ammodytes
P44
183
D.Hlavati,M.Zadravec
FaunistikinalazivodozemacaigmazovauokoliciNaica
FaunisticrecordsofamphibiansandreptilesintheNaicearea
P45
185
D.Jeli,M.Jeli,M.Futo,B.Halpern,K.Ljubisavljevi,N.Tzankov
Molekularna filogenija ivanjskog rovaa (Ablepharus kitaibelii) na sredinjem i sjevernom dijelu
podrujarasprostranjenosti
MolecularphylogenyoftheSnakeeyedSkink(Ablepharuskitaibelii)onthecentralandnorthpartof
itsdistributionrange
186
P46
K.Koller,P.KonradKova
NovidokaziopostojanjutrogloksenihprilagodbiherpetofauneDinarskogkra
NewevidencetoexistenceoftrogloxeneadaptationsinherpetofaunaofDinarickarst
187
P47
Y.Kornilev,F.Brischoux
Coastal population of Dice snakes (Natrix tessellata) helps clarify the evolution of euryhaline
physiologyinmarinetetrapods
P48
187
Y.Kornilev,S.Etem,N.Natchev,I.Ivanchev,N.Tzankov,T.IgnatovaIvanova
ApreliminarystudyonthebioactivityofthemicrobiotainthepostcaecalGItractofthreeTestudo
species(Testudinidae,Testudines)withemphasisonlacticacidbacteria
P49
188
P.Lisiar,B.Lau,I.Vilaj,D.Jeli
Morfolokeznaajkearepoljarice,Hierophisgemonensis(1768,Laurenti)
DistributionandmorphologyofBalkanwhipsnake,Hierophisgemonensis(1768,Laurenti)
P50
189
M.Luka,E.PruknerRadovi,D.Horvatek,I.Cizelj,F.Martinkovi,D.Jeli,.Gottstein
th
12.HBKxxx12 CBC
Praenjezdravstvenogstanjaovjejeribice(Proteusanguinus)uzatoenitvu
Monitoringofhealthstatusoftheolm(Proteusanguinus)incaptivity
190
P51
N.Luki,J.Frii,G.ukalo,R.Deki,B.KukavicaJovanovi
Isoenzymeprofilesofsuperoxidedismutaseandcatalaseinliverandmuscleselectedanuranspecies
P52
191
S.Mekini,N.evrnja,J.Boban,G.Piasevoli,D.Vladovi
ZmijeuherpetolokojzbirciPrirodoslovnogmuzejauSplitu,Hrvatska
SnakesinherpetologicalcollectionoftheNaturalhistorymuseuminSplit,Croatia
P53
191
N.Natchev,S.Handschuh,S.Lukanov,N.Tzankov,B.Naumov,I.Werneburg
Discoveryofanamphikineticamphibianskullanditslinkagetoneoteniclifestyle
P54
192
V.olti,O.Jovanovi,D.Jeli
Zamke smrti neprikladni graevinski zahvati u zatienim podrujima mogu uzrokovati visoku
smrtnostvodozemacaigmazova
Death traps inappropriate constructions in protected areas can cause high mortality in
amphibiansandreptiles
P55
193
G.ukalo,S.orevi,Lj.Tomovi
Sexual dimorphism, diet and reproduction of the grass snake (Natrix natrix) in the region of the
marshypondecosystemBardaa(RepublicofSrpska,BosniaandHerzegovina)
P56
194
E.unje,F.Pasmans,Z.Maksimovi,A.Martel,M.Rifatbefovi
Detection of continued annual mortality in the vulnerable alpine salamander, Salamandra atra
prenjensis(Miki,1969)notassociatedwiththepresenceofknownamphibianpathogens
P57
195
A.Uroevi,K.Ljubisavljevi,A.Ivanovi
PreliminaryassessmentofthelacertidskullmodularityacasestudyofPodarcismuralis
P58
195
I.Vilaj,B.Lau,D.Jeli
Riovka, Vipera berus (Squamata, Viperidae) u Hrvatskoj: populacijska ekologija, odabir
mikrostanitaitermoregulacija
Common adder, Vipera berus (Squamata, Viperidae) in Croatia: population ecology, microhabitat
preferencesandthermoregulation
P59
196
M.Zadravec
GmazoviivodozemciVugrovekeokolice
ReptilesandamphibiansoftheVugrovecarea
P60
197
M.Zadravec,R.Cesarec,Z.Tadi
GmazoviivodozemcikamenolomaBizek
th
12.HBKxxxi12 CBC
ReptilesandamphibiansoftheBizekquarry
200
P61
A.Zimi,D.Jeli,S.Lelo,M.engi
NewrevisionofthechecklistofamphibianfaunainBosniaandHezegovina
Biologijamora
Marinebiology
P62
199
J.Antolovi,E.Coppola,L.Bundone,S.alac,D.kali,V.StingaPerusco,G.HernandezMilan
Uvidioekologijiprehranesredozemnemedvjedice
InsightsontheforagingecologyoftheMediterraneanmonkseal
P63
200
M.Batisti,R.Gari
Brooksialacromaesp.nov.(Tunicata,Thaliacea),novavrstadvootvorkeuJadranskommoru
Brooksialacromaesp.nov.(Tunicata,Thaliacea),anewspeciesofsalpfromtheAdriaticsea
P64
201
J.Boban,D.Vladovi
ZbirkarakovaPrirodoslovnogmuzejauSplitu
NaturalhistorymuseumcrustaceancollectioninSplit
P65
201
S.Bosak,L.upraha,D.Nanjappa,W.Kooistra,D.Sarno
MorfologijaifilogenijarodaBacteriastrum(Bacillariophyta)uJadranskommoru
MorphologyandphylogenyofthegenusBacteriastrum(Bacillariophyta)intheAdriaticsea
P66
202
M.Despalatovi,I.Cvitkovi,A.uljevi
Sastav makrozoobentoskih beskraljenjaka u obratajnim zajednicama u luci Split (Hrvatska,
Jadranskomore)
Composition of macrozoobenthic invertebrates in fouling communities in the harbour of Split
(Croatia,Adriaticsea)
P67
203
N.Jasprica,D.Hafner,A.Car,T.Kapetanovi
Taksonomskisastavmorskihbentoskihdijatomejainjegovodnossglavnimekolokimimbenicima
uBosniiHercegovini
Taxonomic composition of marine benthic diatoms in the relation to some key environmental
variablesinBosniaandHerzegovina
P68
204
P.Krui,P.Rodi,A.Popija,K.Vukovi,P.Ankon,V.Matas,K.Cetini,S.Sviben,A.Kovaev,N.
Moraj
DubinskarasprostranjenostkoraljaukoraligenskojbiocenoziistonogJadrana
DepthdistributionofcoralsinthecoralligenousoftheeasternAdriaticsea
P69
205
M.Mari,M.DeTroch,S.Olenin
th
12.HBKxxxii12 CBC
Trofikeinterakcijeuzatienimmorskimpodrujima:ulogastranihinvazivnihvrsta
Trophicinteractionsinmarineprotecedareas:theroleofinvasivealienspecies
206
P70
S.MatiSkoko,J.Ferri,N.Staglii,P.Tutman,D.BrajiJurica
Dnevni prirasti otolita kao metoda validacije tonog odreivanja starosti trlje od kamena, Mullus
surmuletus
Daily otolith increments as validation method for age determination of the striped red mullet,
Mullussurmuletus
P71
207
J.MioiStoi,G.Plesli,N.RakoGospi,C.M.Fortuna,D.Holcer
Procjenabrojnostizajednicedobrihdupina(Tursiopstruncatus)Vikogakvatorija
Abundanceestimatesofthebottlenosedolphin(Tursiopstruncatus)communityinVisarchipelago
P72
208
B.Musta
Stanjepopulacijeinuna(EngraulisencrasicolusL.1758)uistonomdijeluJadranskogmora
Anchovy(EngraulisencrasicolusL.1758)populationstatusintheeasternAdriaticsea
P73
209
D.PaviiHamer
Praenje radioekolokog stanja sjevernog Jadrana koritenjem dagnje Mytilus galloprovincialis kao
indikatora
Radioecology monitoring of the northen Adriatic sea using mussels Mytilus galloprovincialis as an
inidcator
P74
210
G.Plesli,A.Impetuoso,C.Fortuna
Pojavnostigustoadobrihdupina(Tursiopstruncatus)uMurterskommoru
Occurrenceanddensityofbottlenosedolphins(Tursiopstruncatus)inMurtersea
P75
211
I.Radoni,I.Cvitkovi,M.Despalatovi
Rasprostranjenost, abundancija i razmnoavanje vrste Siphonaria pectinata (Gastropoda,
Pulmonata)uakvatorijugradaSplita
Distribution, abundance and reproduction of the species Siphonaria pectinata (Gastropoda,
Pulmonata)intheSplitcityaquatorium
P76
212
G.Plesli,G.Kett,N.RakoGospi,T.Vuur,D.Holcer,J.MioiStoi,P.Mackelworth
Utjecaj strategije hranjenja na drutvenu strukturu dobrih dupina (Tursiops truncatus) u istonom
Jadranu,Hrvatska
Influence of foraging strategies on social structure of bottlenose dolphins (Tursiops truncatus) in
easternAdriaticsea,Croatia
P77
213
N.RakoGospi,G.Plesli,S.Stipi,J.Corbin,M.Picciulin
Povezanost podvodne buke u cresko loinjskom akvatoriju sa promjenama u glasanju dobrih
dupina(Tursiopstruncatus)
Shiftsinbottlenosedolphin(Tursiopstruncatus)vocalizationinrelationtoseaambientnoiseinthe
CresandLoinjwaters,Croatia
th
12.HBKxxxiii12 CBC
P78
S.Vrgo,N.evrnja
MalakolokazbirkaPetraNovaka
MalacologicalcollectionofPetarNovak
6.SimpozijHrvatskogdrutvazabiljnubiologiju
6thSymposiumoftheCroatianSocietyofPlantBiologists
215
P79
216
M.Curman,M.Tuek,M.Babi,M.Tkalec
Fotosintetska uinkovitost, udio fotosintetskih pigmenata i fenolnih spojeva te antioksidacijska
aktivnosturosika(Droseraceae)prirazliitimuvjetimauzgoja
Photosynthetic efficiency, content of photosynthetic pigments and phenolic compounds, and
antioxidativeactivityinDroseraceaeatdifferentgrowthconditions
P80
D.LeljakLevani,N.Bauer,S.Mihaljevi
Zajednikerazvojneznaajkesomatskeizigotneembriogeneze
Somaticandzygoticembryossharecommondevelopmentalfeatures
217
P81
218
N.Kleteki,T.Vujnovi,E.Kleteki,M.Vukovi
RasprostranjenostdrvenastihinvazivnihvrstaRobiniapseudoacaciaL.,ReynoutriajaponicaHoutt.i
AcernegundoL.napodrujuSamobora
DistributionofnonherbaceousinvasivespeciesRobiniapseudoacaciaL.,ReynoutriajaponicaHoutt.
andAcernegundoL.intheSamoborarea
P82
K.Majsec,M.Pavlica
UlogatransporteraHMAuakumulacijiCduvrstiNicotianaglauca
RoleofHMAtransportersinCdaccumulationinNicotianaglauca
219
P83
220
I.ola,B.Maji,S.Liki,I.JuranoviCindri,G.Rusak
Hranidbenavrijednostkore,plodovaisjemenkioskorue(SorbusdomesticaL.):mineraliifenolnispojevi
NutritionalvalueofSorbusdomesticaL.bark,fruitsandseeds:mineralsandphenolics
P84
221
M.Tuek,M.Curman,M.Babi,M.Tkalec
Fotosintetska uinkovitost, udio fotosintetskih pigmenata i fenolnih spojeva te antioksidacijska
aktivnosturazliitimdijelovimavrevautrihibridarodaSarracenia
Photosynthetic efficiency, photosynthetic pigments, and phenolic compounds, and antioxidant
activityindifferentpitcherpartsofthreeSarraceniahybrids
P85
222
V.Vuji,S.RadiBrkanac,M.RadiStojkovi,S.Ivankovi,J.Hrenovi,M.Rui,B.PevalekKozlina
Antioksidacijska, antibakterijska i citotoksina aktivnost vodenog ekstrakta dubrovake zeine
(CentaurearagusinaL.)
Antioxidant,antibacterialandcytotoxicactivitiesofwaterextractofplantspecies(CentaurearagusinaL.)
th
12.HBKxxxiv12 CBC
P86
M.Vujii,M.Sabovljevi,M.osi,V.Maksimovi,D.Mii,A.Sabovljevi
Dosugarcontentsinselectedmossspeciesimplytotheirsaltresistance?
223
P87
224
M.Zovko,P.Cvjetko,I.VinkoviVrek,B.Balen
Uinaknanoesticasrebranapojavuoksidacijskogstresauklijancimaduhana(Nicotianatabacum)
Theeffectofsilvernanoparticlesonoxidativestressintobaccoseedlings(Nicotianatabacum)
3.SimpozijHrvatskogentomolokogdrutva
3rdSymposiumoftheCroatianEntomologySociety
P88
225
M.Bistrovi,N.Turi,M.Temunovi,G.Vignjevi,E.Merdi
Usporedbauinkovitostimetodauzorkovanjavodenihkukaca(HeteropteraiColeoptera)sobzirom
naatraktant,veliinuvrstaistanite
Acomparisonofmethodsforsamplingaquaticinsects(HeteropteraandColeoptera)consideringthe
bait,bodysizeandhabitattype
P89
226
N.Landeka,I.upi
Rasprostranjenostcrneudovice(Latrodectustredecimgutattus,Rossi1790.)napodrujuHrvatskeiBIH
Distribution of the black widow spider (Latrodectus tredecimgutattus, Rossi 1790.) in the area of
CroatiaandBosniaandHerzegovina
P90
J.Skejo
Faunaskakavaca(Insecta:Orthoptera:Caelifera)Hrvatske
Croatianshorthornedgrasshoppers'(Insecta:Orthoptera:Caelifera)fauna
227
228
P91
A.tih,T.Koren
RaznolikostvretenacaHrvatskogazagorja
DiversityofdragonflyfaunaofHrvatskozagorje
P92
229
I.Vruina,A.Previi,E.Merdi
Novevrstetulara(Insecta:Trichoptera)zafaunuHrvatskeizparkaprirodeKopakirit
Newspeciesofcaddisflies(Insecta:Trichoptera)forCroatianfaunafromtheKopakiritnaturepark
Biologijakopnenihvodaikopna
Biologyoffreshwaterandterrestrialecosystems
P93
230
D. erba, I. Turkovi akali, V. Ergovi, M. Koh, M. Lesjak, A. Petek, A. Vukovi, I. Balkovi, .
Miloevi,F.Stevi
Strukturazajedniceihranidbenenavikeslatkovodnihribauplitkomjezeruumjerenogpodruja
Communitystructureandfeedinghabitsoffreshwaterfishinashallowtemperatelake
231
P94
I.Grigorszky,M.GligoraUdovi,I.Somlyai,B.Palombi,L.Czvek,Gy.Dvai,S.A.Nagy.
th
12.HBKxxxv12 CBC
Relationshipsbetweenalgalproductionandthephysicalandchemicalpropertiesofadrinkingwater
reservoir
231
P95
N.Jasprica,D.Hafner,A.Car,T.Kapetanovi
Taksonomskisastavmorskihbentoskihdijatomejainjegovodnossglavnimekolokimimbenicima
uBosniiHercegovini
Taxonomic composition of marine benthic diatoms in the relation to some key environmental
variablesinBosniaandHerzegovina
P96
232
M.Jeli,T.arevi,K.Jeli,I.Katanovi
Rasprostranjenostiprocjenaveliinepopulacijevidre(LutralutraL.)ualpskojbiogeografskojregiji
Hrvatske
Distributionandestimatedpopulationsizeoftheotter(LutralutraL.)inthealpinebiogeographical
regionofCroatia
P97
233
D.KiNovak,M.Kerovec
BiolokoekolokaobiljejaaluvijalnihpotokaMeimurja
BiologicalecologicalfeaturesofalluvialstreamsoftheregionofMeimurje
P98
235
M. Kuini, G. Mesari, I. Mihoci, E. Vasili, M. Blaevi, T. Koren, T. Finderle, Lj. Ladavac, M.
Grgurev
Vertikalna rasprostranjenost, faunistike i biogeografske znaajke danjih leptira (Insecta,
Lepidoptera)parkaprirodeUka
Verticaldistribution,faunalandbiogeographicalfeaturesofbutterflies(Insecta,Lepidoptera)from
theUkanaturepark
P99
236
M.Ljutina,I.Katanovi,M.Rezo,I.Ivanek,M.Damjanovi,D.Lisii
Sezonske razlike u unosu, skladitenju hrane i teini dinarskog voluhara (Dinaromys bogdanovi) u
zatoenitvu
Seasonal differences in food intake, caching and body mass of the endemic balkan snow vole
(Dinaromysbogdanovi)incaptivity
P100
237
M.Mejdandi,N.Maleevi,N.Koleti,M.Nemet,K.KraljBorojevi
Fitobentosivodenimakrofitikaoelementibiolokekvaliteteiklasifikacijestanitadusubplaninske
krkerijeke
Phytobenthos and aquatic vegetation as biological quality and habitat description elements along
submountaincarsticriver
P101
M.Mrakovi,M.aleta,Z.Mari,D.Zanella,P.Mustafi
ZnaakeihtiofaunerijekeSutle
FishdiversityoftheriverSutla
P102
D.Prli
PoetnaistraivanjavaskularnefloreistanitauzrijekuMuruuHrvatskoj
InitialresearchofvascularfloraandhabitatsalongtheMurariverinCroatia
238
239
th
12.HBKxxxvi12 CBC
240
P103
M.Raan,L.Raan,V.PircMezga
Stanitaslatkovodnihmeduza(CraspedacustasowerbyiLankester1880.)napodrujudonjegMeimurja
FreshwaterjellyfishhabitatsinlowerMeimurje(CraspedacustasowerbyiLankester1880)
P104
241
S.Smiljkov,D.Gusheski,V.SlavevskaStamenkovikj,R.Ilieska,K.Krstevska,S.Stojanovski
QualitativeanalysisofrepresentativesfromAnnelida(OligochaetaandHirudinea)fromKumanovobath
P105
242
F.Stevi,A.Mili,D.poljariMaroni,T.unaPfeiffer,V.Zahirovi,M.Mihaljevi
VertikalnadistribucijamorfolokihoblikavrsteCylindrospermopsisraciborskiiupoplavnomjezeru
VerticaldistributionofmorphologicalformsofCylindrospermopsisraciborskiiinafloodplainlake
P106
243
I.Strbad,V.MietiStankovi,M.Raan,M.Kuini
AntropogeniutjecajnaraznolikostbentikihbeskraljenjakasrednjegtokarijekeDraveipripadajuihljunara
AnthropogenicimpactonbenthicmacroinvertebratediversityinthemiddlereachofDravariverand
theaccompanyinggravelpits
P107
244
B.Vlaievi,J.Vidakovi
Dinamikanaseljavanjaisukcesijetrepetljikaauperifitonujezerapoplavnogpodruja
Colonizationandsuccessiondynamicsofperiphyticciliatesinafloodplainlake
P108
245
P.utini,I.Petri,M.GligoraUdovi,K.KraljBorojevi,I.Mikac,I.Bokan,I.poljari,G.Mri,J.
Kamberovi,A.PlenkoviMoraj,S.Gottstein,I.Ternjej
Mikrobnibiofilmperiodikogslatkovodnogkrkogizvora
Microbialmatsinintermittentfreshwaterkarsticspring
Toksikologijaiekotoksikologija
Toxicologyandecotoxicology
P109
246
S. Babi, R. Sauerborn Klobuar, J. Barii, G. Klobuar, N. Topi Popovi, I. StrunjakPerovi, R.
oRakovac
Procjenautjecajaotpadnogmuljanaokolimoguibiomarkeri
Potentialbiomarkersforsewagesludgepollutionmonitoring
P110
247
S. Babi, R. Sauerborn Klobuar, G. Klobuar, S. Kepec, H. Vii, J. Barii, N. Topi Popovi, I.
StrunjakPerovi,R.oRakovac
Procjena toksinosti komunalnih otpadnih voda iz postrojenja za proiavanje otpadnih voda
koritenjemtestaembriotoksinostinazebricama
Evaluation of toxic potential of municipal waste waters from wastewater treatment plant by
zebrafishembryotoxicitytest
P111
248
G.Gl,A..KarasuBenli,F.Erko,A.Gl
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12.HBKxxxvii12 CBC
249
P112
K.Jurica,S.egan,D.MilojkoviOpsenica,I.BriKaraonji,V.Benkovi,N.Kopjar
Procjenatoksinostivodenogekstraktalistaobineplanike(ArbutusunedoL.)iarbutinanaljudskim
limfocitimaperifernekrviuuvjetimainvitro
Toxicityassessmentofstrawberrytree(ArbutusunedoL.)waterleafextractandarbutininhuman
peripheralbloodlymphocytesinvitro
P113
250
A..KarasuBenli,S.KayiranTunca,P.Arslan,G.Gl,A.SepiciDinel
Determination of acute and sublethal effects of permethrin on narrow clawed crayfish (Astacus
leptodactylusEsch.1823)
P114
251
N.Kopjar,V.Kauba,R.Rozgaj,M.Mladini,M.Mili,D.eljei
Citotoksini,genotoksiniicitogenetikiuinciklorpirifosanaljudskelimfociteperifernekrviuuvjetimainvitro
Evaluation of the cytotoxic, genotoxic and cytogenetic effects of chlorpyrifos in human peripheral
bloodlymphocytesinvitro
P115
252
H.M.Kutlu,D.Vejselova,G.Ku
Determinationofcelldeathmechanismonhumanlungadenocarcinomacellsexposedtoceranib2
P116
253
A.Masla,M.Tkalec,M.Masla
UinakkadmijanafotosintezuisadrajsekundarnihmetabolitauliajevaParmeliasulcata,Evernia
prunastriiElavoparmeliacaperata
Theimpact of cadmiumonphotosynthesis and secondarymetabolitesin lichens Parmelia sulcata,
EverniaprunastriandElavoparmeliacaperata
P117
254
A.Perovi,B.ljuki,S.Perovi,M.rut,G.I.V.Klobuar
PollutionbiomonitoringoftheRiverMoracaandLakeSkadarusingcagedmusselsUniosp.
P118
254
L.Ulm,I.Hrga,A.Krivohlavek
BiolokarazgradnjaorganskihtvariuvodiodabirmetodeHRNENISO9439:2000iliHRNENISO10707:2000
Biological degradation of organic compounds in waterselection of the method HRN EN ISO
9439:2000orHRNENISO10707:2000
P119
255
D.Vejselova,H.M.Kutlu,G.Ku
Investigationofceranib2inducedcytotoxicityonhumanlungadenocarcinomacells
Mikrobiologija
Microbiology
P120
256
A.M.Ladi,A.Mojsoviui
th
12.HBKxxxviii12 CBC
Beznjihsenemoe:eukariotskiorganizmistanovniciaktivnogmulja
Wecan'tdowithoutthem:eucaryoticorganismsinhabitantsoftheactivatedsludge
257
P121
E.iiGuo,N.Krstulovi,S.estanovi
HeterotrofnebakterijeusedimentuiregpodrujaSplita
HeterotrophicbacteriainsedimentofawiderareaofSplit
Komparativnafiziologija,imunobiologijaibiologijaovjeka
Comparativephysiology,immunobiologyandhumanbiology
P122
258
V.Balta,D.Odeh,M.Matakovi,M.Kunti,M.Kukolj,N.Oroli
Animalnimodelpsorijaze:antiupalnaiantioksidativnauinkovitostepigalokatehingalata
Animalmodelsofpsoriasis:antiinflammatoryandantioxidantepigallocatehingallateefficiency
P123
259
S.Blaevi,M.Merkler,D.Hamidovi,D.Hranilovi
Separacijska anksioznost u mladunaca takora s perinatalno promijenjenim metabolizmom
serotonina
Separationanxietyinratpupswithperinatallyalteredserotoninmetabolism
P124
260
D.iki,R.Fuchs,S.MatiSkoko,D.Skaramuca,Z.Frani,I.Landeka,B.Skaramuca
Razlikeusastavumasnihkiselinaumurine,ugoraijeguljeizJadranskogmorablizuDubrovnika
Differences in fatty acid composition between Moray, Conger and Common eel from Adriatic sea
nearDubrovnik
P125
262
M.Kukolj,M.Kunti,R.Raan,E.Roak,N.Oroli
UinkovitostkafeinskekiselinenaperitonealnuangiogenezuuEhrlichovomascitesnomtumoru
TheefficiencyofcaffeicacidonoxidativestressandangiogenesisinEhrlichascitestumor
P126
263
M.Kunti,M.Kukolj,N.Oroli
GalnakiselinasmanjujetumorskirastiangiogenezuumievasEhrlichovimascitesnimtumorom
GallicacidreducestumorgrowthandangiogenesisinmicewithEhrlichascitestumor
P127
264
A.evo,S.ogori,P.Stani,A.HorvatKneevi,V.Benkovi
PovezanostivotnihnavikaiprimarnihoteenjaDNAspermijasreproduktivnomsposobnostimukaraca
Relationship between lifestyle habits and primary DNA damage of the sperm and reproductive
abilityofmen
P128
265
N.Oroli,D.iki,J.Nemrava,P.Majcen,M.Kunti,M.Kukolj,E.Roak,E.Golua
Uinkovitostkvercetinanabiomarkerezaosteoporozu
Theeffectivenessofquercetinonbiomarkersforosteoporosis
P129
266
N.Oroli,M.Kunti,M.Kukolj,I.oli
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12.HBKxxxix12 CBC
267
P130
N.Oroli,M.Kunti,M.Kukolj,D.Sirovina
Uinakflavonoidaihipertermalneintraperitonealnekemoterapije(HIPEC)narasttumoraiindukciju
mikronukleusaumijemmodelutumora
Effectofflavonoidsandhyperthermalintraperitonealchemotherapy(HIPEC)ontumourgrowthand
micronucleusinductioninmousetumourmodel
P131
268
N.Oroli,M.Kunti,M.Kukolj,D.Sirovina,I.opf
ZatitniireparacijskiuinakpentadekapeptidaBPC157nastanicekrvi,jetreibubregamia
ProtectiveandreparativeeffectofpentadecapeptideBPC157onmiceblood,liverandkidneycells
P132
269
N.Oroli,D.Odeh,M.Matakovi,V.Balta,M.Kunti,M.Kukolj,K.Sailo,D.iki
Kvercetinsmanjujeiritacijukoeinduciranuheksilsalicilatomilidinpropildisulfidom:oksidativnistresiupala
Quercetin attenuates nhexyl salicylate or dinpropyl disulfideinduced skin irritation: oxidative
stressandinflammation
P133
270
D.Sirovina,N.Oroli,M.ZovkoKoni,G.Gregorovi
Naringeninublaavapatolokepromjeneujetrimaibubregumievasdijabetesom
Naringeninamelioratespathologicalchangesinliverandkidneyofdiabeticmice
Genetika,staninaimolekularnabiologija
Genetics,cellularandmolecularbiology
P134
272
I.Maguire,M.Jeli,G.Klobuar,A.tambuk,M.Vuci,F.Grandjean
Primjena analize mikrosatelitnih biljega na populacijama vrsta Astacus astacus i Astacus
leptodactylusuHrvatskojpreliminarnirezultati
ApplicationofmicrosatelliteanalysisonAstacusastacusandAstacusleptodactyluspopulationsfrom
Croatiapreliminaryresults
P135
273
M.Matei,S.Jonji,I.Jurak
IstraivanjeulogemikroRNAkodherpessimpleksvirusa1
InvestigatingtherolesofmicroRNAsinherpessimplexvirus1infection
P136
274
D.Perina,M.Korolija,M.PopoviHadija,I.Grbea,R.Belui,M.Imeek,C.Morrowe,T.Bakran
Petricioli,A.Miko,H.etkovi
Karakterizacijagena/proteinaFAUizmorskespuveSuberitesdomuncula
CharacterizationofFAUgeneandproteinfrommarinespongeSuberitesdomuncula
P137
275
D.Perina,A.Miko,J.Ahel,H.etkovi,R.aja,I.Ahel
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12.HBKxl12 CBC
Distribucijaproteinaukljuenihupoli(ADPribozil)acijuusvimdomenamaivota
Distributionofproteinpoly(ADPribosyl)ationsystemsacrossalldomainsoflife
276
P138
P139
278
I.Radoni,.Trumbi,T.egviBubi,L.Grubii,I.Mladineo
Genetska analiza populacije atlantske plavoperajne tune, Thunnus thynnus (Linnaeus, 1758) u
kaveznomuzgojuprimjenommikrosatelitnihlokusa
Geneticanalysisofcagerearedatlanticbluefintuna,Thunnusthynnus(Linnaeus,1758)population
byapplicationofmicrosatelliteloci
P140
279
I.Tomljanovi,K.Skube,D.Pavokovi
Ekspresija i identifikacija novih proteina djelomino neureene tercijarne strukture (IDP) iz biljke
uronjak(Arabidopsisthaliana)nakonizlaganjaabiotikomstresu
ExpressionandidentificationofnovelintrinsicallydisorderedproteinsinArabidopsisthalianaduring
abioticstress
P141
280
T.VojvodaZeljko,E.atovi,M.Plohl
Genomikoljkaaukazujunatranspozicijskumobilnostsatelitnihponavljanja
BivalvegenomessuggesttranspositiondrivenmobilityofsatelliteDNArepeats
P142
281
I.Bonjak,A.Bielen,M.Jakli,M.Cvitani,K.Sepi,T.Simi,J.Lajtner,S.Hudina,T.VojvodaZeljko,E.
atovi,M.Plohl
Razlikeuotpornostinaantropogenistresizmeuinvazivneinativnevrstekoljkaa
Differencesintolerancetoanthropogenicstressbetweeninvasiveandnativebivalves
P143
282
A.Zubkovi,I.Ratkaj,I.Jurak
IspitivanjeulogestaninihmiRNAureplikacijiherpessimpleksvirusa1
InvestigatingtherolesofhostmicroRNAsinherpessimplexvirus1infection
Kazaloautora/IndexofAuthors
284
Sponzori/Sponsors
296
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PLENARNAPREDAVANJA
PLENARYLECTURES
Plenarnapredavanja
Plenarylectures
PL1
PROTEININTERACTIONNETWORKSINHEALTH&DISEASE
I.tagljar
Departments of Biochemistry and Molecular Genetics, Donnelly Centre, University of
Toronto,Canada
Inthepastdecade,numerouscomprehensiveproteomicsstudieshaveallowedresearchers
to generate detailed proteinprotein interaction ("interactome") maps, providing an
unparalleledglobalviewoftheinterplaybetweenthedifferentsystemswithinthecell,and
greatly increasing our understanding of the physiological mechanisms involved in both
normal and disease states. My lab is focused specifically on understanding how the
interactions of membrane proteins contribute to cellular disease states at a systems level.
Despite extensive proteomics research in the past decade, there is a lack of indepth
understandingofproteinnetworks associatedwithintegralmembraneproteinsbecauseof
their unique biochemical features, enormous complexity and multiplicity. This is a major
obstacletounderstandingthebiologyofderegulationofintegralmembraneproteinswhich
leadstonumeroushumandiseases,andconsequentlyhindersourdevelopmentofimproved
and more targeted therapies to help treat these diseases. To address this problem, my lab
hasdevelopedtwouniquetechnologiesspecificallysuitedforthestudyoffulllengthintegral
membraneproteinsintheirnaturalcellularcontext;theclassicMembraneYeastTwoHybrid
(MYTH) 15 and the newly created Mammalian Membrane TwoHybrid(MaMTH)6. Our
ultimate goal is to uncover a wealth of information about protein interactions for the
majority of "druggable" human membrane proteins, which should in turn greatly facilitate
the discovery of new truths about diseases like cancer, schizophrenia, cystic fibrosis,
hypertension and Parkinsons disease. During my talk, I will discuss our recent findings
indicatingthattheapplicationofMaMTHtothehumanEpidermalGrowthFactorReceptor
(EGFR)resultedintheidentificationofCrkIIproteinasanovelinteractorofoncogenicEGFR
(L858R),andshowedthatCRKIIpromotespersistentactivationofaberrantsignalinginnon
small cell lung cancer (NSCLC) cells. I will also illustrate how MaMTH is a powerful tool for
investigating dynamic interactomes of human integral membrane proteins and why it
promisessignificantcontributionstotherapeuticresearch.
PL2
THERISEANDFALLOFGENOMECOMPLEXITYANDOTHERMACROEVOLUTIONARYSTORIES
R.Bakari,T.DomazetLoo
RuerBokoviInstitute,Zagreb,Croatia
Recent advances in understanding of evolutionary life cycle of genes reveal that gain and
lossofgenefamiliesismajorfactorintheevolutionofgenomecomposition.However,itis
unclear how gene family birthanddeath rates fluctuate at the macroevoutionary scale.
Consequently it is obscure how genome complexity changed during evolutionary time in
different lineages. Here, using phylostratigraphic approach and newly developed
bioinformatic toolkit, we measured gene family birthanddeath rates at the phylogenetic
tree comprising more than 600 eukaryotic genomes. We found that, in terms of time,
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PL3
DIVERGENCEANDCONSTRAINTINTHEORIGINOFNEWSPECIES
P.Nosil
RoyalSocietyofLondon,UniversityofSheffield,UnitedKingdom
Theoriginofnewspeciescreatesbiologicaldiversityandunderstandingspeciesformationis
thus a key goal in biology. In this talk, I will tackle the issue of why some populations that
begin the speciation process diverge further than others, a phenomenon central to
understanding diversification. Using a combination of theoretical modeling and empirical
studies of plantfeeding insects I will show how adaptation to different ecological
environments generally promotes speciation. However, this process can be constrained or
counteracted by numerous factors. Specifically, speciation can stall 'partway' before
completion due to: an insufficient number of genetic differences underlying adaptive
divergence, ecological shifts that are too modest to drive strong divergence, and selective
processesthatincreasegeneticmixingbetweenpopulations.Theoriginofnewdiversitythus
reflectsabalancebetweenthesefactorsdrivingandconstrainingevolutionarydivergence.
PL4
SOCIOSCIENTIFICISSUES:ACHALLENGEFORBIOLOGYTEACHERS
A.orgo
FacultyforNaturalSciencesandMathematics,UniversityofMaribor,Maribor,Slovenia
Inmanycontemporarydocumentsisaddressedneedfordifferentkindofeducation,where
outcomeofsuchapproachisacitizenwhonotonlypossesssolidknowledgefoundationbut
alsoisabletouseachievedknowledgetosolverealworldpracticalorintellectualproblems
even after finished formal education. In documents underpinning education emphasis is
giventodevelopment ofskillslike criticalthinking,creativity,ability tosolveproblemsand
softskillslikeabilitytolearn,toactivatetacitknowledgeortoworkingroups.Realandyet
unsolvedchallengeishowtoorganize instructionstoachieveat least a part oflistedgoals
balancing between welltested practices what worked in the past and enthusiasm of
untested novelties. Because we cannot treat instructions and school activities as from the
societyisolated and valuefree systems, Biology teachers cannot avoid teaching and follow
up classroom discussions of sensitive themes. Beside already "traditional" themes like
environmental education is nowadays in the middle of public interest biotechnology with
themeslikegeneticengineering,geneticallymodifiedorganisms (GMOs),foodfromGMOs,
therapeutic and reproductive cloning, substitute maternity, cloning of human, impact of
GMOs on human health, health of animals, and environment, etc. To cover the social and
scientific aspects of such the term "socioscientific issue" was coined. From the socio
scientific view, such issues are frequently controversial and, especially in cases when they
becomethesubjectofpublicinterestdebates,areoftenbackedupwithlimitedknowledge
and strong attitudes. In educational practice, such public debates are reflected in the
classroom, where teachers are challenged by complex issues, where proficiency in one
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12.HBK312 CBC
discipline is in most cases insufficient to answer students curiosity. When such a theme is
assigned to a Biology teacher, probably the most important obstacle is that teachersoften
lackappropriateknowledgeaboutthesocialsideoftheissue.
PL5
PLANTRESPONSETOABIOTICSTRESS
D.BarZvi
BenGurionUniversity,BeerSheva,Israel
Being sessile organisms that cannot relocate, plants are continuously exposed to the ever
changing environment. To accommodate to these changes, plants evolved intensive
molecular, biochemical and physiological mechanisms to respond to hostile environments.
These changes include a global change in the transcriptome and proteome. I will discuss
three molecular examples for plant response to drought and salt stress: (a) Salt stress
impairschloroplaststructureandaffectsthisorganellesgeneexpressionandgenomecopy
number.(b)Transcriptionfactorsarekeyplayersinreachingnewsteadystatesintranscript
levels. The stress hormone, abscisic acid, regulated ABI4 transcription factor plays central
role in root biology under stress and nonstress conditions. ABI4 modulates the activity of
the Na+ transporter HKT1;1 and controls lateral root formation. (c) The Ubiquitin
Proteasome System (UPS) is a major pathway for controlled protein degradation in all
eukaryotes,includingplants.Proteinsdescendedfordegradationarecovalentlymarkedbya
shortubiquitinchain,byathreesteppathway.Thethirdstep,iscatalyzedbyalargefamily
ofsubstratespecificubiquitinligases,isbelievedtobethemodulatedstepinthedecisionof
proteinfate.Wehaveidentifiednewubiquitinligasesinvolvedinplantresponsetoabiotic
stress,andstudytheirroleinplantresponsetotheenvironment.
PL6
ANALYSES OF MORPHOLOGY, PHYSIOLOGY, AND MICROBIAL DIVERSITY AFTER A RECENT
DIETARYSWITCHINALIZARD
A.Herrel
UMR7179C.N.R.S/M.N.H.N.,Dpartementd'EcologieetdeGestiondelaBiodiversit,Paris,
France
Althoughevolutioniscommonlyconsideredaslowprocess,recentevidencehasshownthat
organisms can show dramatic and measurable phenotypic responses after introductions to
novel environments in relatively short time spans. We have previously shown how lizards
(Podarcis sicula) have rapidly evolved differences in head morphology, bite strength, and
digestive tract structure after experimental introduction onto a small island in the Adriatic
Sea,Croatia.Despitetheshorttimescale(36years)sincethisintroduction,theintroduced
lizards became omnivores and evolved caecal valves in the hindgut, a structure rarely
observed in lizards. These changes in morphology and performance parallel those typically
documentedamongspeciesandevenfamiliesoflizardsinboththetypeandextentoftheir
specialization. Here, we present novel data on 1) the morphology of the cranium and its
muscles using CT scanning and 3D geometric morphometric approaches, 2) the digestive
physiology of the two populations, and 3) the diversity of the microbiomes in the hindgut
usingmetagenomicsequencingapproaches.
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PL7
HOW MUCH ROOM IS LEFT FOR CONVENTIONAL BIOLOGY IN MODERN NANOSCALE AND
OMICSRESEARCH
D.Drobne
DepartmentofBiology,Biotechnicalfaculty,Ljubljana,Slovenia
Traditionalbiologistsweretrainedtoworkataspecificlevelofbiologicalcomplexity,and/or
to investigate a specific group organisms. But now, many biologists call themselves
integrativebiologists.Alsoacademicunitsandstudyprogramshavechangedtheirnamesto
Integrative biology. What is "Integrative Biology"? Integrative biology has different
definitions. It could be defined as multidisciplinary/crossdisciplinary research of biological
systems. Multidisciplinarity means bringing together researchers from different, areas of
expertiseorusingadiversityoftechniquesandapproachestoaddressparticularquestions
(Wake 2003; Joyce and Palsson 2006). Integrative approaches seek both diversity and
incorporation. These approaches deal with integrations across all levels of biological
organization,frommoleculestothebiosphere,andwithdiversityacrosstaxa,fromviruses
toplantsandanimals.Manyofthequestionsnowbeingaddressedbybiologistsrequireboth
reductionistic and incorporative elements to contribute to an answer to a larger problem.
Biologists are coming to the realization that our ability to deal with questions of biological
complexity would benefit from high throughput systems biology, referred to as "omics"
technology.Integrated"omics"approacheshavecreatedexcitingopportunitiesforbiological
researches.Highthroughputstudiesofbiologicalsystemsarerapidlyaccumulatingawealth
ofomicsscaledata.However,thisabundanceofinformationalsopresentsmanyobstacle.
Themainoneincludesextractionofdiscernablebiologicalmeaningfrommultipleomicsdata
sets.Inthepresentation,twoexamplesonsuccessfulintegrationofconventionalbiological
study, nanotoxicity data and omic approach in understanding the effects of engineered
nanomaterialsonmodeltestorganisms(DapniamagnaandTetrachymenatermophyla)are
presentedindetail.Atpresent,onecanconcludethat,theomicstechnologiesarelikelyto
continue its expansion for all fields in biological but when integrating omicdata with the
traditionalapproachesthedifficultproblemsonpreviouslyunprecedentedscalesandcould
betackled.Apparently,itisallaboutintegrating,inbiology.Integrativebiology.
PL8
GLYCANS AS INTEGRATORS OF GENES AND ENVIRONMENT AN OFTENIGNORED LAYER
OFBIOLOGICALCOMPLEXITY
G.Lauc
UniversityofZagrebFacultyofPharmacyandBiochemistry
12.HBK512 CBC
Individualvariabilityinglycomecompositionisverylarge,butglycosylationofanindividual
proteinseemstobeunderstronggeneticinfluence,withtheheritabilityofthe(forexample)
IgG glycome being up to 80%. Structural details of the attached glycans are of great
physiologicalsignificanceandmanypathologicalconditionsareassociatedwithvarioustypes
ofglycanchanges.Sincetheonsetofgenomewideassociationstudies(GWAS),thousandsof
geneticlocihavebeenassociatedwithdifferentdiseasesandtraits.However,inthelastfew
years,andparticularlyafterrecentpublicationoftheresultsfromtheENCODEproject,itis
becomingincreasinglyclearthatGWASstudiesareonlyabeginningoftheunderstandingof
complex human diseases. Hypotheses generated in these studies have to be put in the
context of complex biology of life and a more elaborate approach that combines different
omics phenotypes is needed to understand disease mechanisms and perform patient
stratificationthattranscendsgenomics.Glycomics,asbyfarthemostcomplexepiproteomic
modification, has an immense potential in this respect, which is only beginning to be
investigated.
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USMENAPRIOPENJA
ORALPRESENTATIONS
Konzervacijskabiologija,zatitaprirodeiokolia
Conservationalbiology,natureandenvironmentprotection
O1
VRIJEDNOSTFAUNISTIKIHISTRAIVANJAKAOTEMELJAZAPLANIRANJEAKTIVNEZATITE
PRIRODEPROJEKTINTEGRACIJEUEUNATURA2000(NIP)
I.Sui1,L.Katui2
1
Ministarstvozatiteokoliaiprirode,Zagreb,Hrvatska(ivana.sucic@dzzp.hr)
2
Odjelzadivljeiudomaenesvojteistanita,Dravnizavodzazatituprirode,Zagreb,Hrvatska
ProjektintegracijeuEUNatura2000(NIPprojekt)jestruniprojektsektorazatiteprirodekroz
koji se prikupljaju postojei te novi podaci o rasprostranjenju vrsta iz odabranih prioritetnih
taksonomskihskupina.Prvomfazomprojektapredvienojeprikupljanjeliteraturnihimuzejskih
podataka za 13 taksonomskih skupina, a kroz drugu terensko prikupljanje recentnih podataka i
laboratorijska analiza za 9 prioritetnih taksonomskih skupina. U okviru projekta izrauje se i
detaljna karta prirodnih i poluprirodnih neumskih stanita Hrvatske. Primjer skupine
Plecoptera,prikazujuvanostfaunistikihprojekatazaplaniranjeaktivnezatiteprirode.Prema
Crvenompopisuobalara(2007)uHrvatskojjebilopoznato28vrstaobalara,aDravnizavodza
zatitu prirode je raspolagao s 152 nalaza obalara. Obradom dostupnih literaturnih referenci i
muzejskihnalaza,teterenskihistraivanjau2014.i2015.godiniukupnosuprikupljenipodacio
3979nalazaza110vrsta.
Kljunerijei:aktivnazatitaprirode,faunistikaistraivanja,NIPprojekt
I.Sui1,L.Katui2
1
MinistryofEnvironmentalandNatureProtection,Zagreb,Croatia(ivana.sucic@dzzp.hr)
2
Department for Wild and Domesticated Taxa and Habitats, The State Institute for Nature
Protection,Zagreb,Croatia
EU Natura 2000 Integration Project (NIP project) is a professional project of nature protection
sector, through it will be gathered existing and new data about distribution of species of the
selectedprioritytaxonomicgroups.Thefirstphaseoftheprojectforetellgatheringofliterature
and museum data for 13 taxonomic groups, and throughout the second gathering recent data
and laboratory analysis for 9 priority taxonomic groups. As a part of the project it will be also
made a detailed map of natural and seminatural nonforest habitats of Croatia. Example on
taxonomic group Plecoptera is showing the importance of faunistic projects in planning active
nature protection. According to the Red list of stoneflies (2007) in Croatia it was known 28
stoneflie species, and State Institute for Nature Protection disposed with 152 findings of
stoneflies. Analysing the available literature references and museum findings, and also field
researchfindingsin2014and2015,atotalof3979findingsfor110speciesweregathered.
Keywords:activenatureprotection,faunisticresearch,NIPproject
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12.HBK812 CBC
O2
ASSESSINGPROTECTEDAREAVALUESANDBENEFITSINTHEDINARICARC
A.tefan1,S.Stolton2,D.Porej1,K.Z.Ivani1
1
WWFAdria,Zagreb,Croatia(astefan@wwf.panda.org)
2
EquilibriumResearch,Bristol,UK
WWFrecentlycompletedaprotectedareasbenefitassessmentin58parks,representingover
50%ofallprotectedterritoryintheDinaricArc.TheProtectedAreaBenefitsAssessmentTool
(PABAT) assesses the full range of legal benefits arising from protected areas, using a
structured questionnaire in a participatory way. The PABAT looks at issues ranging from
ecosystem services such as clean water, local products, sustainable fisheries, to tourism and
lessquantifiablevalues.Inmanyofthenationalandnatureparkstheworkshophasbeenthe
first opportunity for local communities, water and energy companies, tourism experts and
fisher folk to meet park staff and discuss what the protected area means to them. In many
cases,managersandotherworkshopparticipantshavebeenunawareofthebenefitsthatare
important to other stakeholders. The PABAT has been appli ed in about 60 PAs in eight
countries,fromSloveniatoAlbania;certainlythelargestassessmentofprotectedareabenefits
in the world to date. The results will inform protected area management plans, rural
development, interpretation and education programmes to help influence day to day
management decisions. We will present an update of the PABAT methodology and
consolidated results, discuss lessons learned about its use at this scale to help other users;
outlinehowthePABATresultscanfeedintoPAmanagementandpolicy;andlookatstrategic
implicationsfortheDinaricArc.
Keywords:ProtectedAreaBenefitsAssessmentTool(PABAT),DinaricArcParks,WWF
O3
PRVAPRIMJENARADARSKEORNITOLOGIJENAPODRUJUBALKANSKEREGIJE
E.Patev1,M.Grgurev2,A.Avdakovi3,M.Masla4,A.Bukovac1,H.Peternel1
1
Geonaturad.o.o.zastruneposlovezatiteprirode,Zagreb,Hrvatska(epatcev@geonatura.hr)
2
Gekomd.o.o.geofizikalnoiekolokomodeliranje,Zagreb,Hrvatska
3
JPElektroprivredaBiH,Sarajevo,BosnaiHercegovina
4
Oikond.o.o.Institutzaprimijenjenuekologiju,Zagreb,Hrvatska
12.HBK912 CBC
Kljune rijei: radar, migracija ptica, radarska ornitologija, vertikalna distribucija leta, softver
Distance
THEFIRSTAPPLICATIONOFTHERADARORNITHOLOGYINTHEBALKANREGION
E.Patev1,M.Grgurev2,A.Avdakovi3,M.Masla4,A.Bukovac1,H.Peternel1
1
GeonaturaLtd.,Zagreb,Croatia(epatcev@geonatura.hr)
2
GekomLtd.GeophysicalandEcologicalModelling,Zagreb,Croatia
3
JPElektroprivredaBiH,Sarajevo,BosniaandHerzegovina
4
OikonLtd.InstituteofAppliedEcology,Zagreb,Croatia
Basedondatacollectedfrombothradarsystemandafieldornithologistssurveys,aprecise
approximation of the bird migration patterns can be made, especially on data such as birds
flightdirection,altitudeandintensity.ThesurveywasconductedinBosnaandHerzegovina,on
Vele mountain. The main goal was to collect bird activity and movements data with radar
system in order to investigate possible migration corridor over the area.For collection of
migrationdatatwoXbandmarineradarswereusedsimultaneously,workingintwodifferent
modes (horizontal and vertical). Data was collected in the period from 24th August to 26th
September 2014. The radar data (signals on the radar screen) were captured using a "frame
grabber" card and recorded to a computer. Collected data consisted of several distance
measurements in relation to radar antennas including Euclidean distance, altitude and land
distance. To count a distancedependant probability of radar signals, a correction factor was
calculated using Distance software. Results showed seasonal and diurnal difference of
migration intensity. Use of radar system greatly contributes to better understanding of
migrationpatternsespeciallyverticaldistributionofmigrationandmigrationdirection.Inorder
toacquirecompletedataonbirdmigration,itisnecessarytointegratetheradarsystemwith
standardornithologicalresearchmethods.Ideally,thiswouldbesubstantiatedwithlocaland
regionalmeteorologicaldata.
Key words: radar, bird migration, radar ornithology, migration pattern, flight attitude, Distance
software
O4
MEUNARODNO VANA PODZEMNA SKLONITA ZA IMIE HRVATSKOJ PRIMJENA U
OUVANJUIZATITI
D.Hamidovi,S.Desnica
Dravnizavodzazatituprirode,Zagreb,Hrvatska(daniela.hamidovic@dzzp.hr)
12.HBK1012 CBC
vrstaimiakojijeusklonituzabiljeenizatitasklonita(teakpristup,zatvorenovratima,
otvoreno za turistike potrebe). Za svaku vrstu u sklonitu naveden je godina u kojoj je
zabiljeennajveibrojjedinkiod1994.godinetenainkoritenjasklonita.Ukupnojeuovim
podzemnim sklonitima zabiljeeno 25 vrsta imia od 34 prisutne u Hrvatskoj. U pravilu
najvee hibernacijske kolonije nalaze se u kontinentalnoj biogeografskoj regiji, dok su u
mediteranskojnajveeporodiljnekolonijeveinevrsta.UekolokojmreiNatura2000nalaze
sesvih55sklonitatejezasvezahvate,planoveiprogramepotrebnosagledatiutjecajnaciljne
vrste imia. Tada se provode predvieni zakonski mehanizmi koji na razini stratekog
planiranja, prostornog plana ili samog zahvata uzimaju u obzir tip sklonita, nain koritenja
sklonitateekologijeciljnevrsteimia.Predstavljamokriterijeiprimjereocjeneutjecajana
razini strateke ocjene upanijskog prostornog plana te nekoliko ocjena zahvata
vjetroelektrana.
Kljunerijei:imii,podzemnasklonita,zakonodavstvo,ouvanje,glavnaocjenautjecaja
D.Hamidovi,S.Desnica
StateInstituteforNatureProtection,Zagreb,Croatia(daniela.hamidovic@dzzp.hr)
Keywords:bats,undergroundroosts,legislation,conservation,appropriateassessment
O5
SUSTAV ZA MULTISPEKTRALNU ANALIZU VEGETACIJE ZA BIOLOKA I EKOLOKA
ISTRAIVANJA
D.K.Hackenberger,T.Vondrak
Odjelzabiologiju,SveuiliteJosipaJurjaStrossmayera,Osijek(domagojhack@gmail.com)
Istraenajemogunostuporabeautonomnogleteegureaja(ALU)zaprouavanjevegetacije
izzrakazapotrebebiolokihiekolokihistraivanja.Prilikomistraivanjarazvijenjejednostavni
imobilnisustavzadaljinskooitavanjevegetacijskihindeksa(NDVI,EVI,SAVI/MSAVI).Ovakav
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12.HBK1112 CBC
sustav omoguava efektivno i brzo prikupljanje podataka o vegetaciji iz zraka. Sama tehnika
detektiranja vegetacijskog indeksa temelji se na dvjema jednakim komercijalnim HD
kamerama. Jedna od kamera je bila modificirana za detekciju valnih duljina u blisko
infracrvenompodruju(nearinfrared,NIR)dokjedrugasnimalavalneduljinevidljivesvjetlosti.
Slike dobivene kamerama su se pohranjivale i obraivale tijekom leta ime je omogueno
neposredno koritenje rezultata u daljnjim znanstvenim istraivanjima. Preliminarna analiza
dobivenih podataka pokazuje veliki potencijal za koritenje ovakvog sustava u biolokim i
ekolokimistraivanjima.
Kljunerijei:vegetacijskiindeksi,NIR,ALU,kamera,daljinskooitavanje
D.K.Hackenberger,T.Vondrak
Departmentofbiology,JosipJurajStrossmayerUniversity,Osijek(domagojhack@gmail.com)
Possibilityofusageofunmannedaerialvehicle(UAV)forairbornevegetationobservationfor
demand of biological and ecological studies has been researched. Throughout the study a
simple andmobile systemforremotesensing ofvegetationindices(NDVI,EVI,SAVI/MSAVI)
hasbeendeveloped.Thiskindofsystemfacilitateseffectiveandrapidaccumulationofaerial
vegetationdata.TheaforementionedtechniqueisbasedontwoidenticalconsumergradeHD
cameras.Oneofthesecamerashasbeenmodifiedtodetectnearinfraredwavelengths(NIR),
while the additional camera recorded visible spectrum wavelengths. Images given by the
cameraswerebeingstoredandprocessedduringtheflight,thisenabledpointblankusagein
furtherscientificresearch.Preliminaryanalysisofgivendatashowspromisingpotentialforuse
ofthiskindofsysteminbiologicalandecologicalresearch.
Keywords:Vegetationindices,NIR,UAV,Camera,Remotesensing
O6
ENDEMICANDRELICTSSPECIESINSPECIALNATURERESERVEZASAVICA
M.Stankovi
Pokret gorana Sremska MitrovicaSRP Zasavica, Sremska Mitrovica, Serbia
(zasavica@zasavica.org.rs)
Thetertiaryrelicsofthefloraofthereserveare:Stratiotesaloides,Trapanatansagg.,Butomus
umbellatus, Erithronium denscanis, Humulus lupulus, Hidrocharis morsusranae, Isopirum
thalictroides, Loranthus europaeus, Tamus communis, Viscum album and Aldrovanda
vesiculosa. The Special Nature Reserve ''Zasavica'' is the only habitat in Serbia for the plant
Aldrovanda vesiculosa, which was long thought to be extinct in Serbia. The three species of
flora of the reserve are glacialrelicts Urtica kioviensis, Scirpus triqueter, Thelypteris palustris,
andAchilleaasplenifoliaisthePannoniansubendemic.Thefaunaofthereservehasrecorded
fourendemicandrelictsspeciesofinvertebratesandvertebrates.Frominvertebratesthereis
found a rare endemic branchiopods shrimp Chirocephalus brevipalpis and a relic living fossil
Lepidurus apus. The entomofauna of the reserve has two endemic species Stenopterus
similatusandZeunerianaamplipenniswhichisfoundagainaftermorethanthirtyyears.From
theclassofGastropodatherewasidentifiedaBalkanendemicspeciesofTandoniakusceri.A
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specialrarityofthereserveisthegloballyendangeredrelictspeciesUmbrakrameriforwhich
theflowZasavicaisoneofthethreeremaininghabitatsinSerbia,andthissubpopulationisone
ofthemostendangeredinthewholecomplexinEurope.Thefaunaofamphibiansandreptiles
presentthreeBalkanendemicsTriturusdobrogicus,Lacertaagilisssp.bosnicaandViperaberus
bosniensis(blackmelanisticforminthereserve)whichinPosavinahasarelictcharacter.
O7
LIAJEVIKAOBIOINDIKATORIKVALITETEZRAKANAPODRUJUMOTOVUNSKEUME
M.Masla1,A.Partl2,Z.Mesi3,O.Antoni4,5
1
Geonatura d.o.o. za strune poslove zatite prirode, Zagreb, Hrvatska
(mmaslac@geonatura.hr)2Dravnizavodzazatituprirode,Zagreb,Hrvatska
3
Oikond.o.o.Institutzaprimjenjenuekologiju,Zagreb,Hrvatska
4
Oikon d.o.o. Institut za primijenjenu ekologiju/Geonatura d.o.o. za strune poslove zatite
prirode/GekomGeofizikalnoiekolokomodeliranjed.o.o.,Zagreb,Hrvatska
5
Odjelzabiologiju,SveuiliteJ.J.StrossmayerauOsijeku,Osijek,Hrvatska
Liajevisumeunajpoznatijimbiolokimindikatorimazaprocjenukvalitetezrakajersvuvodu
potrebnu za ivot u cijelosti primaju iz zraka. Obzirom na navedeno, mogu se koristiti za
procjenu stanja okolia uzimajui u obzir vie izvora zagaenja i razliite efekte (eng. multi
pollutant/multieffect). Odreivanjem vrsta i brojnosti liajeva, mogue je mapirati uinke
dugoronog(kumulativnog)oneienjazrakanavelikimpodrujima.Koritenjemliajevakao
bioindikatora odreena je kvaliteta zraka na podruju Motovunske ume u sklopu projekta
"PlanrestauracijestarogkoritaMirneBiolokoekolokaistraivanjaipodloge".Na18ploha
je izvreno uzorkovanje liajeva te su odreene prisutne vrste i njihova uestalost. Prema
prisutnim vrstama i njihovoj abundaciji, zakljueno je kako se gotovo cijelo podruje odlikuje
iznimno visokom kvalitetom zraka kao i visokom koliinom vlage u zraku. Istraivanjem je
utvreno kako u Motovunskoj umi, u kojoj su debla veinom prekrivena mahovinom, nije
mogueodreditikvalitetuzrakapremastandardiziranojmetodizaodreivanjekvalitetezraka
pomouliajevauEuropi.StogasezavlaneumeslineMotovunskojumipredlaekoritenje
metodekojasetrenutnoprimjenjujeuSADu,anakojuneutjeekoliinamahovina.Rezultati
potvruju primijenjivost novih ekolokih indeksa za slina istraivanja kvalitete zraka i opeg
stanjaistraivanogpodruja.
Kljunerijei:liajevi,oneienjezraka,kvalitetazraka,bioindikatori,vlaneume
LICHENSASBIOINDICATORSOFAIRQUALITYINTHEMOTOVUNFORESTAREA
M.Masla1,A.Partl2,Z.Mesi3,O.Antoni4,5
1
GeonaturaLtd.,Zagreb,Croatia(mmaslac@geonatura.hr)
2
StateInstituteForNatureProtection,Zagreb,Croatia
3
OikonLtd.InstituteofAppliedEcology,Zagreb,Croatia
4
Oikon Ltd. Institute of Applied Ecology /Geonatura Ltd. /Gekom Ltd.Geophysical and
ecologicalmodelling,Zagreb,Croatia
5
BiologyDepartment,J.J.StrossmayeraUniversityofOsijek,Osijek,Croatia
Lichensareamongthebestknownbiologicalindicatorsforassessingtheairquality,dueto
theirabilitytofullyreceiveallwaternecessaryforlifedirectlyfromtheair.Hence,theyare
important because they can be used to estimate the multipollutant/multieffect
environmentcondition.Bydeterminingthespeciesandthenumberoflichens,itispossible
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tomaptheeffectsoflongterm(cumulative)airpollutioninlargeareas.Byusinglichensas
bioindicatorstheairqualityintheMotovunforestareawasdeterminedaspartofthe"Plan
for the restoration of the Mirna river old bed Biological and ecological research and
background".Onthe18plotslichensweresampledandpresentspeciesandtheirfrequency
wasdetermined.Itwasconcludedthatalmosttheentireareaischaracterizedbyextremely
highairquality,aswellasahighamountofairmoisture.Thesurveyidentifiedshortcomings
intheexistingstandardizedmethodfordeterminingairqualityusinglichensinEurope,when
workinginahumidforestwithhighmossabundancyonthetreetrunk.Whenworkinginthe
woods with humidity levels comparable to this area, it is proposed to use the method
currently used in the USA that is not biased with the moss on the tree trunk. The results
confirmtheapplicabilityofnewenvironmentalindicesforsimilarstudiesofairqualityand
generalconditionofthestudyarea.
Keywords:lichens,airpollution,airquality,bioindicators,humidforest
O8
UTJECAJ METEOROLOKIH PARAMETARA NA VARIJACIJE DVOSATNIH KONCENTRACIJA
PELUDNIHZRNACAEMPRESA(CUPRESSACEAE),GRADZADAR,HRVATSKA
A.Vuci1,D.PeroPucar1,B.Miti2,I.Hrga3,B.Pucar1
1
Slubazazdravstvenuekologiju,ZavodzajavnozdravstvoZadar,Zadar,Hrvatska(avucic@zjz
zadar.hr)
2
Biolokiodsjek,Priridoslovnomatematikifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
3
Sluba za zatitu okolia i zdravstvenu ekologiju, Nastavni zavod za javno zdravstvo "Dr.
Andrijatampar",Zagreb,Hrvatska
Peludizporodiceempresa(Cupressaceae)najbrojnijajepeluduatmosferigradaZadrasudjelom
odgotovo50%uukupnombrojupeludnihzrnacauzraku.Smatrasedapeludnazrncaizporodice
empresa imaju umjeren do visok alergeni potencijal te oni spadaju u glavne alergene na
mediteranskom podruju. Svrha istraivanja je utvrditi dvosatne fluktuacije peludnih zrnaca iz
porodice empresa u danima sa zabiljeenom visokom koncentracijom (> 90 pz/m3) te utvrditi
utjecaj nekih meteorolokih parametara na dvosatne varijacije peludnih zrnaca empresa.
Istraivanje je provedeno tijekom 2007. 2009. na temelju preporuka EAN / REA. Aerobioloki
uzorci suprikupljenipomouautomatskog sedmodnevnog volumetrijskoguzorkivaa tipaHirst.
Dobivenirezultatipokazujudajepeludizporodiceempresa(Cupressaceae)prisutnauatmosferi
grada Zadra tijekom cijele godine, ali veina peludnih zrnaca empresa zabiljeena su tijekom
zimskih mjeseci (od veljae do travnja). Na varijacije dvosatnih koncentracija peludnih zrnaca
empresastatistikiznaajanipozitivanutjecajimajutemperatura,brzinavjetraibrojsunanih
sati,dokjeutjecajrelativnevlagezrakastatistikiznaajaninegativan.
Kljunerijei:aerobiologija,Cupressaceae,dvosatnekoncentracije,Hirst,Zadar
12.HBK1412 CBC
CupressaceaepollenisthemostabundantpollenintheatmosphereofZadarwithashareof
almost50%intotalpollencount.TheallergenicpotentialofCupressaceaepollenismoderate
tohighanditrepresentsthemajorairborneallergeninMedierraneanarea.Thepurposeofthis
studywastoestablishtwohourintervaldailyfluctuationsofCupressaceaepollenduringdays
withmarkedhighconcentration(>90pg/m3)andtodetermineinfluenceofsomemeterological
parametersondiurnalCupressaceaepollenvariation.Thestudywascarriedduringthe2007
2009 using the Spanish Aerobiological Network (EAN/ REA) recommendation. Aerobiological
samples were collected via Hirsttype, seven day volumetric sampler. The obtained results
showed that the Cupressaceae pollen is present in the Zadar atmosphere during the whole
year, but the majority of Cupressaceae pollen is marked during the winter months (from
February till April). Correlations between Cupressaceae pollen diurnal concentration and
meteorologicalparameterswerestatisticallysignificant:influenceoftemperature,windspeed
andhoursofsunshinewerepositiveandinfluenceofrelativehumiditywerenegative.
Keywords:aerobiology,Cupressaceae,diurnalconcentration,Hirst,Zadar
O9
MLADICA (Hucho hucho) NA BALKANU: RASPROSTRANJENOST I BUDUI UTJECAJI
HIDROENERGETSKIHPLANOVA
MladicajejednaodnajzagonetnijihslatkovodnihribaEurope.Povijesno,ovajevrstabilairoko
rasprostranjena itavim dunavskim slijevom. Od druge polovine 19. stoljea, ukupna se
brojnostmladicesmanjilazadvijetreinepasupreostalepopulacijejakougroene,posebice
razvojem hidroelektrana. U ovom je radu pregledano stvarno podruje rasprostranjenosti
mladice na Balkanu. Utvrena su 1842 km rijeka u kojima postoji samoodriva populacija
mladice, ime je ovo podruje postalo arite za ovu vrstu. Ove se populacije nalaze u 43
razliite rijeke ili odvojena rijena podruja u Sloveniji, Hrvatskoj, Bosni i Hercegovini, Srbiji i
Crnoj Gori. Oko 65% svih rijeka koje nastanjuje mladica nalaze se u ovim dravama, to
naglaava vanost balkanskih rijeka za preivljavanje ove vrste. Najvea je prijetnja ovim
populacijama plan gradnje hidroelektrana. Ukupno su identificirana 93 projekta za brane
izravno na rijekama u kojima ivi mladica i veliki broj dodatnih projekata na pritocima ili
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12.HBK1512 CBC
vodamauzvodnoodsadanjegstanitamladice,kojienedvojbenodegradiratistanineuvjete
nizvodno.Akoseoviplanoviizvedu,predviamodaenajmanje6070%balkanskihpopulacija
i3540%svjetskepopulacijemladicebitiizgubljeno.Nadalje,preostaleepopulacijebitimalei
fragmentirane,toeimonemoguitidugoronopreivljavanje.
Kljunerijei:mladica,Balkan,hidroenergija
THEHUCHEN(Huchohucho)INTHEBALKANREGION:DISTRIBUTIONANDFUTUREIMPACTS
BYHYDROPOWERDEVELOPMENT
TheHuchenisoneofthemostenigmaticspeciesofEurope'sfreshwaterfauna.Historically,the
specieswaswidespreadacrosstheentireDanubebasin.Sincethelate19thcentury,however,
Huchen populations declined by two thirds and the remaining populations are now highly
endangered by hydropower development. In this study, we review the actual occurrence of
HuchenintheBalkanregion.Atotalof1842riverkmsupportingselfsustainingpopulationsof
Huchenintheregionhavebeenidentified,makingittheglobalhotspotforthespecies.These
populationsarefoundin43riversordistinctriverreachesinSlovenia,Croatia,BIH,Serbiaand
Montenegro. About 65% of all Huchen rivers globally are located in these countries,
highlightingtheimportanceofBalkanRiversforthesurvivalofthespecies.Themajorthreatto
thesepopulationsisamassivehydropowerdevelopmentplan.Atotalof93damprojectswere
identifieddirectlyinriverreachessupportingHuchenandalargenumberofadditionalprojects
arelocatedintributariesorheadwaterreachesupstreamofHuchenhabitatthatwillinvariably
degradeenvironmentalconditionsdownstream.Iftheseplansarecarriedout,wepredictthat
atleast6070%oftheBalkanpopulationandabout3540%oftheglobalpopulationofHuchen
would be lost with the remaining populations being small and severely fragmented and
eventuallynolongerabletosurviveinthelongterm.
Keywords:Huchen,Balkan,hydropower
O10
PROCJENAUSLUGAEKOSUSTAVANAPODRUJUDRAVAMOLVEPREMATRIPOTENCIJALNA
SCENARIJA
D.Pithart1,A.Dupli2,P.Kutlea2,I.PetrovRani2,N.Trenc2
th
12.HBK1612 CBC
Beleco,Prag,eka
Odjel za ocjenu prihvatljivosti zahvata za prirodu, Dravni zavod za zatitu prirode, Zagreb,
Hrvatska(aljosa.duplic@dzzp.hr)
PoplavnenizineSave,DraveiDunavauHrvatskojpodutjecajemsurazliitihljudskihaktivnosti,
ukljuujuiizgradnjunaselja,poljoprivredu,prometiproizvodnjuenergije.Onetakoerimaju
velik znaaj u pruanju pitke vode, zatiti od poplava, kao stanita brojnih vrsta, u produkciji
drvnemase,ribeidivljai.Stogainformacijeofunkcijamaiuslugamaekosustavatogpodruja
valjauzetiuobzirprilikomizradaprostornihplanova,razliitihstrategijatedonoenjaodlukau
javnomiprivatnomsektoru.Istraivanjejeobuhvatilo38kmrijenogtokaDrave,kodopine
Molve, s poplavnom nizinom ukupne povrine 201 km2. Cilj je bio dodatno argumentirati
potrebu za ouvanjem rijenih ekosustava kroz promoviranje prirode kao osnove za razvoj.
Analizirane su razliite opskrbne, regulacijske, podravajue i kulturoloke funkcije. Njihove
vrijednosti procijenjene su prema tri potencijalna scenarija koritenja ovog podruja u
budunosti: A odravanje postojeeg stanja, B provedba planiranih projekata (izgradnja HE
Molve1i2)iCrazvojkrozodrivokoritenjeprostoratoukljuujeproirenjepoplavnezone.
Koritenemetodeovisilesuovrstiuslugeioulaznimpodacima.TemeljzaanalizuuscenarijuB
bili su utjecaji i promjene na uslugama ekosustava uzrokovane postojeim HE na Dravi u
Hrvatskoj. Scenarij C pokazuje najviu vrijednost za tri kljune usluge ekosustava: proizvodnji
drva,osiguravanjustanitaiublaavanjupoplava,dokjeutjecajscenarijaBpretenonegativan.
Kljunerijei:Drava,uslugeekosustava,procjena,HEMolve
D.Pithart1,A.Dupli2,P.Kutlea2,I.PetrovRani2,N.Trenc2
1
Beleco,Praha,CzechRepublic
2
Department for nature impact assessment, State Institute for Nature Protection, Zagreb,
Croatia(aljosa.duplic@dzzp.hr)
Floodplains of Sava, Drava and Danube Rivers in Croatia are a target of a variety of human
activities,suchassettlements,agricultureandtransportorenergyproduction.Also,theyhave
potential to provide drinking water, flood protection, habitats for numerous species, timber
production as well as fish and game animals. Information about ecosystem functions and
services of this area should be taken into consideration duringpreparation of physicalplans,
strategiesandbypublicandprivatesectordecisionmaking.Inresearchisincluded38kmof
the Drava River, in municipality Molve and its floodplain, altogether 201 km2. The main goal
wastogetadditionalargumentationsforpurposeofriverineecosystemconservationthrough
promotion of nature as a basis for development. Regulating, supporting, provisioning and
culturalfunctionswereanalyzed.Theirvalueswereevaluatedaccordingthreepossiblefuture
scenarios: A the status as it is, B the business as usual (with HP Molve 1 and 2) and C
sustainabledevelopmentcharacterizedbyextendingofactivefloodplainzone.Usedmethods
dependontypeofservicesanddataintegrity.ForthescenarioBwereanalyzedimpactsand
changesonecosystemservicesasconsequencesofthethreeexistingHPontheDravaRiverin
Croatia.ThescenarioCprovidesthehighestvalueforthethreekeyservices:woodproduction,
habitats provision and flood mitigation. On the other hand the impact of the scenario B is
mostlynegative.
Keywords:Drava,ecosystemservices,assessment,HPMolve
2
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12.HBK1712 CBC
O11
MODELIRANJE POPULACIJE VRSTE SLABO ISTRAENIH KARAKTERISTIKA PRIMJER
POPOVSKEGAOVICE(Delminichthysghetaldii)NAPODRUJUOMBLE
B.K.Hackenberger,O.Antoni,.Lonari
Zavod za kvantitativnu ekologiju, Odjel za biologiju, Sveuilite Josipa Jurja Strossmayera u
Osijeku(hack@biologija.unios.hr)
Matematikimodelisusastavnidiosvakogsuvremenogrjeavanjaproblemauokoliujerosim
pronalaenja matematikih zakonitosti problema modeli mogu u veoj ili manjoj mjeri
sadravatipredikcijskusnaguiskoristivuzadonoenjeodlukateplaniranjeimonitoringmjera
zatiteprirode iokolia.Popovskagaovica(Delminichthys ghetaldii)jeugroenavrstaaranki
kojaobitavanairempodrujuhercegovakogijunodalmatinskogkra.Zapotreberjeavanja
jednogodokolinihproblemazahvataHEOmbla,bilojepotrebnoustanovitidaliprimjerciove
vrste,kojiobitavajunapodrujuizvoritarijekeOmble,inesamostalnupopulacijuilisusamo
dio vee populacijske cjeline (metapopulacije). Posebna tekoa u rjeavanju ovog problema
inilajeinjenicadajepopovskagaovicarelativnoslaboistraenaidajevrlomalodostupnih
podataka.Natemeljupostojeihpodataka,osobnihiskustavaistraivaainjihovogmiljenjate
teorije,nainjenojenekolikohipoteza.Primjenomdiskretnogmatrinogmodeliranjausprezis
metodama opetovanog podatkovnog uzorkovanja, utvrdile su se vjerojatnosti svake od
pretpostavljenih hipoteza. Pokazano je da je popovska gaovica prisutna na podruju izvorita
rijekeOmblediopunoveepopulacijetedabizahvatHEOmbla,baremtosetieopstanka
ovevrstenatompodruju,imaopozitivanuinak.
Kljunerijei:dinamikapopulacija,popovskagaovica,Ombla
MODELINGPOPULATIONOFAPOORLYINVESTIGATEDSPECIESEXAMPLEONDelminichthys
ghetaldiiINTHERIVEROMBLA
B.K.Hackenberger,O.Antoni,.Lonari
Subdepartment of Quantitative Ecology, Department of Biology, Josip Juraj Strossmayer
University(hack@biologija.unios.hr)
Mathematical models are part of every modern environmental problem solving process.
Besidesfindingmathematicalprinciplesmodelscanhaveastrongpredictionpowerthatcanbe
usedforplanningandmonitoringpurposesandcanbeusefulforpolicymakers.Delminichthys
ghetaldiiisendangeredspeciesofcyprinidfamilythatcanbefoundonwideHerzegovinianand
thesouthernDalmatiankarstarea.Inordertosolveoneoftheenvironmentalproblemsofthe
project HPP Ombla, it was necessary to determine whether specimens of this species that
inhabitthespringareaoftheOmbla,constituteanindependentpopulationorareonlypartof
alargerpopulation(metapopulation).Specialdifficultiesinsolvingthisproblemaccountedfor
thefactthattheDelminichthysghetaldiiisrelativelypoorlyinvestigatedspeciesandthatthere
was very little information available. Based on existing data, personal experiences of
researchers and their opinions and theories, we made several hypotheses. By using discrete
matrix modeling in conjunction with the methods of resampling, we determined the
probability of each of the proposed hypothesis. It has been shown that the Delminichthys
ghetaldii population that is present in the Ombla spring area is part of the larger population
and that a project HPP Ombla, at least concerning the survival of this species, could have a
positiveeffect.
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12.HBK1812 CBC
Keywords:populationdynamics,Delminichthys,Ombla
O12
U POTRAZI ZA OUVANIM RIJEKAMA: IDENTIFIKACIJA NAJZNAAJNIJIH RIJEKA ZA ZATITU
PRIRODEUHRVATSKOJ
K.ganec1,F.Antonelli2,I.PopoviDujmovi3,N.Jeran4
1
Sveuilite u Zadru, Odjel za nastavnike studije u Gospiu, Gospi, Hrvatska
(kzganec@unizd.hr)
2
WWFMediteranskiProgram,Rim,Italija
3
WWFAdria,Zagreb,Hrvatska
4
Ministarstvozatiteokoliaiprirode,Zagreb,Hrvatska
Ovaj rad je prvi pokuaj da se sveobuhvatno ocijeni ekoloko stanje i konzervacijska vanost
hrvatskihrijeka.Ciljpredstavljenemetodologijejeodreivanjenajznaajnijihrijekazaouvanje
cjelokupne slatkovodne bioraznolikosti u Hrvatskoj. Upotrijebili smo kombinaciju
ekoregionalnogpristupasglavnimciljempredstavljanjasvihsastavnicabioraznolikostipojedine
ekoregije u kombinaciji s odreivanjem ekolokog stanja rijeka. Nakon utvrivanja tipologije
odreenesuglavnejediniceanalize:OcjenjivaniRijeniSegmenti(ORS).Ekolokostanjesvakog
ORSa odreeno je kombinacijom pet kriterija: hidrologija, morfologija korita, kvaliteta vode,
riparijska zona i koritenje zemljita. Ekoloko stanje svakog ORSa dobiveno je zbrajanjem
ocjena svih kriterija (maks. vrijednost 50). Podaci o rasprostranjenosti ugroenih i endemskih
ribaiprisutnostizatienihpodrujakoritenisuzaprioritizacijuORSauodlinomilidobrom
stanju. Ukupno smo analizirali 82 rijeke, ukupne duine toka 4522 km u tri ekoregije:
panonskoj, dinaridskokontinentalnoj i dinaridskomediteranskoj. Ukupna duina toka
odabranih rijeka od najvee vanosti iznosi 1607 km (22%), a dodatni ORSi, koji obuhvaaju
ukupno998kmtokaodabranisukakobiseosiguralareprezentativnostsvihrijenihtipovau
svakoj ekoregiji. Odabrani ORSi predstavljaju najznaajnije rijene segmente, ija e zatita
osiguratidugoronoouvanjenajznaajnijihsastavnicaslatkovodnebioraznolikostiuHrvatskoj.
K.ganec1,F.Antonelli2,I.PopoviDujmovi3,N.Jeran4
1
University of Zadar, Department of Teachers' Education in Gospi, Gospi, Croatia
(kzganec@unizd.hr)
2
WWFMediterraneanProgrammeOffice,Rome,Italy
3
WWFAdria,Zagreb,Croatia
4
MinistryofEnvironmentalandNatureProtection,Zagreb,Croatia
Environmental status and conservation values of Croatian rivers have never been
comprehensively assessed. We present a methodology for the identification of the most
pristineriversofhighconservationvaluewhichareimportantfortheprotectionoffreshwater
biodiversity in Croatia. The methodology is a combination of ecoregional conservation
approach where representation of all distinct biodiversity features is a key goal, and
assessmentofriverreachcondition.Mainunitsofanalysis,theEvaluatedRiverReaches(ERRs),
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12.HBK1912 CBC
wereidentifiedafterallrivertypesweredistinguished.TheconditionofeachERRwasassessed
usingacombinationoffivecriteria:hydrology,channelmorphology,riparianvegetation,water
qualityandlanduse.ThefinalconditionofERRwasscored(max.50)bysummingscoresfor
thefive criteria. The distribution of endangered freshwater f ish speciesandprotected areas
wasusedforprioritizingtheERRsinhighandgoodcondition.Altogether82riversand4522km
ofriverlengthwereanalyzedinthreeecoregions:Pannonian,DinaricContinentalandDinaric
Mediterranean.ThetotallengthofveryhighpriorityERRswas1607km(35%),whileadditional
ERRs comprising 998 km (22%) of river length were included to assure representation of all
river types in each ecoregion. The selected ERRs represent priority river reaches whose
protection should ensure the long term persistence of the most important features of
freshwaterbiodiversityinCroatia.
Keywords:freshwaterbiodiversity,ecoregionalconservation,typology,condition,rivers
O13
PROTOKOLPROCJENERIZIKAODINVAZIVNOSTISTRANIHVRSTAKORNJAAUHRVATSKOJ
A.Dupli1,D.Jeli2,B.Bari1,B.JanevHutinec3,P.Kutlea1,I.Cizelj4
1
Odjel za ocjenu prihvatljivosti zahvata za prirodu, Dravni zavod za zatitu prirode, Zagreb,
Hrvatska(aljosa.duplic@dzzp.hr)
2
Hrvatskiinstitutzabiolokuraznolikost,HrvatskoherpetolokodrutvoHyla,Zagreb,Hrvatska
3
JavnaustanovaMaksimir,Zagreb,Hrvatska
4
UstanovaZoolokiVrtGradaZagreba,Zagreb,Hrvatska
Trgovina kunim ljubimcima jedan je od putova unosa stranih vrsta u prirodu. Meu
najpoznatijim primjerima invazivne strane vrste koja je na taj nain proirena svijetom je
crvenouha kornjaa (Trachemys scripta elegans). Nakon to je trgovina njome u Hrvatskoj
zabranjena, dolo do znaajnog smanjenja broja kornjaa na tritu te je nastala potreba za
iznalaenjem drugih pogodnih vrsta. Da bi se dozvolilo stavljanje na trite novih vrsta
potrebnojeiskljuitipostojanjeekolokogrizikausluajudavrstadospijeuprirodu.Buduida
ne postoji standardna procedura za procjenu rizika od invazivnosti za kornjae, na temelju
analizakriterijaizliteratureispecifinostiuHrvatskoj,zatujesvrhuizraenprotokol.Prema
protokolu,kriterijizaprocjenurizikamogusepodijelitiuetirigrupekojeobuhvaajupodatke
o rasprostranjenosti vrste u prirodi i podrujima u kojima je vrsta kao strana uspostavila
populaciju, o biolokim i ekolokim obiljejima vrste, podatke o povijesti invazivnosti te
podatke o dostupnosti vrste na tritu EU. Analiza rizika ukljuuje i procjenu klimatske
pogodnosti stanita za to je izraen model s kartografskim prikazom. Pitanja su zatvorenog
tipa te su im pridruene vrijednosti od 0 do 3. to je vrijednost nia to je potencijalni rizik
manji. Od analiziranih vrsta one s najmanjim rizikom invazivnosti su Graptemys versa,
Sternotherus carinatus i Ocadia sinensis dok su one s najveim rizikom Apalone ferox, A.
spiniferaiPelodiscussinensis.
Kljunerijei:kornjae,stranevrste,procjenarizika,invazivnost
PROTOCOLFORTHEINVASIVENESSRISKOFTHEALIENTURTLESPECIESINCROATIA
A.Dupli1,D.Jeli2,B.Bari1,B.JanevHutinec3,P.Kutlea1,I.Cizelj4
1
Department for nature impact assessment, State Institute for Nature Protection, Zagreb,
Croatia(aljosa.duplic@dzzp.hr)
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12.HBK2012 CBC
CroatianInstituteforBiodiversity,CroatianHerpetologicalSocietyHyla,Zagreb,Croatia
PublicinstitutionMaksimir,Zagreb,Croatia
4
ZooZagreb,Zagreb,Croatia
Thetradeinpetsisoneofthemainpathsforintroductionofalienspeciesinnature.Themost
widely known example of the invasive species that has spread worldwide in this way is
Trachemysscriptaelegans.AfterthetradewiththisspecieswasbannedinCroatiathenumber
ofturtlesonthemarkethassignificantlydecreasedandtheneedtofindsuitablespeciesfor
themarkethasoccurred.Topermitthetradewithanewspeciesitisnecessarytoexcludethe
ecological risks in the case if it enters nature. As there is no standard procedure to assess
invasivenessriskforturtlesaprotocolwascreatedforthispurpose,basedonthecriteriafrom
literature and the specifics of Croatia. In accordance with the protocol criteria for risk
assessment can be divided in four groups that include the data about distribution of the
species in nature and in the areas in wh ich it has introduced and established a population,
data about biological and ecological characteristics of the species, data about history of
invasiveness and data about the availability of the species on the EU market. Risk analysis
includesassessmentofclimatesuitability.Thequestionsareoftheclosedtypewithattributed
values from 13. The lower the attributed value the potential risk is lower. From analysed
species, the species with the least invasiveness risk are Graptemys versa, Sternotherus
carinatus i Ocadia sinensis and with the highest risk are Apalone ferox, A. spinifera and
Pelodiscussinensis.
Keywords:turtle,alienspecies,riskassessment,invasiveness
O14
KAKO SE BORITI PROTIV INVAZIVNE VRSTE? BIOLOKO SUZBIJANJE KESTENOVE OSE
IKARICEUNESENOMVRSTOMPARAZITOIDA
D.Matoevi1,I.Frani2,I.Luki1
1
Hrvatskiumarskiinstitut,Jastrebarsko,Hrvatska(dinkam@sumins.hr)
2
Cuglini1,Zagreb,Hrvatska
Kestenova osa ikarica (Dryocosmus kuriphilus) je invazivna vrsta kukca u cijelom svijetu i
izuzetnobrzoseiriunovomstanitu.Smatrasejednimodnajznaajnijihtetnikanapitomom
kestenu(Castaneasativa).Autohtonevrsteparazitoidakojesutrofikivezaneuzhrastoveose
ikarice (Cynipidae) su se prilagodile ovom novom invazivnom domainu, ali ne mogu
uinkovito utjecati na smanjenje gustoe njegove populacije. Bioloko suzbijanje koristei
unesenuvrstuparazitoidaTorymussinensissepokazalokaojedinauinkovitametodakontrole
populacije kestenove ose ikarice, usporavajui njezino irenje i smanjujui tete na
podnoljivu razinu. Visoka specifinost parazitoida T. sinensis upuuje na ogranieni i
nepostojeipotencijaliskoritavanjaautohtonihdomaina.UHrvatskojseu4godineodpojave
kestenoveoseikaricenaovognovogdomainaprilagodilo15vrstaautohtonihparazitoida,
ali oni nemaju nikakvog utjecaja na smanjenje gustoe njezine populacije. Zbog toga je u
prirodne sastojine pitomog kestena u Hrvatskog isputen uneseni parazitoid T. sinensis kao
dokazano uinkovito sredstvo biolokog suzbijanja. U izlaganje se prikazuju rezultati uzgoja i
isputanjaparazitoidaT.sinensisuHrvatskoj.
Kljune rijei: Dryocosmus kuriphilus, Torymus sinensis, autohtoni parazitoidi, invazivna vrsta
kukca
3
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12.HBK2112 CBC
HOW TO CONTROL INVASIVE SPECIES? CLASSICAL BIOLOGICAL CONTROL OF Dryocosmus
kuriphilusWITHINTRODUCEDPARASITOIDSPECIES
D.Matoevi1,I.Frani2,I.Luki1
1
CroatianForestResearchInstitute,Jastrebarsko,Croatia(dinkam@sumins.hr)
2
Cuglini1,Zagreb,Croatia
Dryocosmus kuriphilus is a globally invasive insect pest, spreading very quickly in new
habitatsandmakingseriouslydamagetosweetchestnut(Castaneasativa)forestsinCroatia.
Indigenous parasitoid species trophically associated with oak gallwasps (Cynipidae) have
adapted to this new invasive host but cannot effectively regulate its population density.
ClassicalbiologicalcontrolusingintroducedparasitoidTorymussinensishasbeenprovento
betheonlyeffectivemethodofcontrollingthepopulationsofD.kuriphilus. T.sinensiscan
successfully control the population density of D. kuriphilus, slowing down the spread and
mitigating negative impact of this invasive chestnut pest and keeping the damage of D.
kuriphilus at acceptable level. High specificity of T. sinensis suggests that it has limited
potentialofexploitingnativehosts.InCroatia,during4years15speciesofnativeparasitoids
haveadaptedtonewinvasivehostbuthavenoimpactonloweringthepopulationdensity.
T. sinensis has been released in sweet chestnut forests in Croatia as promising biological
control agent. Results on laboratory rearing and release of T. sinensis in Croatia are
presented.
Key words: Dryocosmus kuriphilus, Torymus sinensis, native parasitoids, invasive insect
species
O15
TETEIKORISTIINVAZIJAUNEENIHRIBAUSLIVUNERETVE
B.Glamuzina
Odjel
za
akvakulturu,
Sveuilite
u
Dubrovniku,
Dubrovnik,
Hrvatska
(branko.glamuzina@unidu.hr)
Recentna istraivanja ukazuju da brojnost veine autohtonih vrsta opada kao rezultat
aktivnosti ovjeka ("gubitnici"), a zamjenjuje ih znaajno manji broj invazivnih vrsta u
promijenjenim ekosustavima ("dobitnici"). Ovo je posebice vidljivo u slatkovodnim
ekosustavima Mediterana s visokom razinom endemizma riba, obiljeenoga uskim arealom
veine vrsta. Jedno od vanih podruja mediteranskoga endemizma riba je sliv Neretve s
opisanih dvadeset stenoendemskh vrsta. Neretva je najdulja rijeka istonojadranske obale
(230km),kojaizvireuBosniiHercegovini,autjeeirokommovarnomdeltomuHrvatskoj.
Poslije 1960. godine rijeka je regulirana izgradnjom pet brana i formiranjem pet umjetnih
akumulacija. Donji tok je melioriran, te je veina movara i laguna pretvorena u
poljoprivredno zemljite. Sve ove promjene dovele su do formiranja ekosustava novih
znaajka u odnosu na prirodne, te su se autohtone vrste riba nale pred izazovima
prilagodba ili nestajanja. Dodatno su novonastali ekosustavi poribljavani nizom alohtonih
vrsta, te je do danas zabiljeeno dvadeset pet uneenih vrsta riba. Novija istraivanja su
pokazala znaajno poveanje brojnosti uneenih i smanjivanje brojnosti autohtonih vrsta
riba.Ovopredavanjeopisatidananjustrukturupopulacijaribaunekimekosustavima,dati
listu pobjednika i gubitnika, teta i koristi u ekosustavima i ribarstvu i predloziti mjere za
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12.HBK2212 CBC
ublaavanjenajznaajnijihprijetnja.
Kljunerijei:invazijeriba,koristiitete,slivNeretve
B.Glamuzina
Department for Aquaculture, University of Dubrovnik, Dubrovnik, Croatia
(branko.glamuzina@unidu.hr)
Recent evidences show that most species are declining as a result of human activities
("losers") and are replacing by a much smaller number of expanding species that thrive in
man altered environments ("winners"). This is the case in freshwater ecosystems of the
Mediterraneanregion,whereahighlevelofendemismmakesnativefishextinctionsmuch
more likely because of the small natural ranges. One of these hotspots of Mediterranean
endemismistheNeretvaRiverwatershed,with20stenoendemicspeciesrecentlydescribed
there.TheNeretvaRiveristhelongestriveroftheEasternAdriaticwatershed,230kmlong,
originating in Bosnia and Herzegovina. Only the most downstream 20 km, which are
wetlandsandtheestuary,areinCroatia.Sincethe1960stheriveranditsflowisregulated
due to the construction of five hydroelectric power dams leading to the formation of five
reservoirs. The lower part was reclaimed and significant areas or wetlands and shallow
lagoons were converted to agricultural land. This provided a completely new ecological
situation, creating problems for the survival of the native fish fauna. The recent list of
introduced fish in the Neretva River includes 25 species. Recent studies showed significant
emergingofintroducedfishpopulationsandsignificantdecreaseofthenativespecies.This
presentation will discuss new fish community structure, present list of the winners and
losersanddescribemeasurestomitigatemostsignificantthreats.
Keywords:fishinvasions,costsandbenefits,Neretvawatershed
O16
PROTOKOL ZA DOJAVU I DJELOVANJE U SLUAJU PRONALASKA UGINULIH, BOLESNIH ILI
OZLIJEENIHSTROGOZATIENIHMORSKIHIVOTINJA
K.Jeli,J.Jeremi
Odjel za divlje i udomaene svojte i stanita, Odsjek za more, Dravni zavod za zatitu
prirode,Zagreb,Croatia(info@dzzp.hr)
UJadranskommoruobitavanekolikovrstakitova,dupinaimorskihkornjaa.Utjecajljudskih
aktivnosti poput ribarstva i turizma ine te vrste osobito ranjivima. Uzimajui u obzir
migracijskuprirodu ovihvrsta tezajedniku odgovornostdravanaJadranu,suradnjajeod
velike vanosti za planiranje uinkovite i dugorone strategije zatite te je zbog navedenog
pokrenut meunarodni projekt IPA Adriatic NETCET. Dravni zavod za zatitu prirode je u
okviru razvoja nacionalnog Sustava za dojavu i djelovanje jo 2010. godine zapoeo u
suradnji s Dravnom upravom za zatitu i spaavanje (112) rad na uspostavi Protokola za
dojavu i djelovanje u sluaju pronalaska uginulih, bolesnih ili ozlijeenih strogo zatienih
morskih ivotinja. U rad protokola do danas su ukljueni obalne i otone veterinarske
ambulante,MOCPula,Hrvatskiveterinarskiinstitut,VeterinarskifakultetuZagrebu,teodjeli
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12.HBK2312 CBC
islubeuupanijamaodgovornizaEcobrodice.Vrstezakojesenajeeprimajudojavesu
dobri dupin (Tursiops truncatus) i glavata elva (Caretta caretta). U proteklih pet godina
zaprimljenesuidojavezaplavobijelogdupina(Stenellacoeruleoalba),krupnozubogdupina
(Ziphius cavirostris) i kornjau sedmoprugu usminjau (Dermochelys coriacea). Svi podaci
skupljeni putem postojeeg Protokola upisuju se u bazu i analiziraju, dio su monitoringa
vrsta,dajuuviduprijetnjeipomauuplaniranjuuinkovitijezatite.
Kljunerijei:Jadran,protokol,zatienemorskevrste,kitovi,morskekornjae
K.Jeli,J.Jeremi
Departement for Wild and Domesticated Species and Habitats, Sea Section, State Institute
forNatureProtection,Zagreb,Croatia(info@dzzp.hr)
Several species of cetaceanas, dolphins and sea turtles are recorded for the Adriatic Sea.
Impact of human activities such as fishing and tourism makes those species highly
vulnerable. Due to the migratory nature of those species crossborder collaboration and
shared management responsibility between Adriatic states is crucial in order to plan
effective longterm conservation strategies. For that purpose, an international IPA Adriatic
project NETCET is launched. Since 2010, in the frame of National Alerting and Monitoring
System, State Institute for Nature Protection started cooperation with National Protection
and Rescue Directorate (112) on development of Protocol for Alerting and Monitoring of
dead, sick or injured strictly protected marine species. At the moment, Protocol involves
veterinary ambulances on the coast and islands, MEC Pula, Croatian Veterinary Insti tute,
Faculty of Veterinary Medicine and County offices responsible for ecovessels. The most
reportedspeciesareBottlenosedolphin(Tursiopstruncatus)andLoggerheadturtle(Caretta
caretta). In the last five years there have also been reports on Striped dolphin (Stenella
coeruleoalba), Cuvier's beaked whale (Ziphius cavirostris) and Leatherback turtles
(Dermochelyscoriacea).AlldatacollectedthroughtheexistingProtocolareregisteredinthe
database and analysed, they are the part of the monitoring of species, and they give an
insightintothreatsandhelpintheplanningofmoreeffectiveprotection.
Keywords:Adriatic,Protocol,protectedmarinespecies,cetaceans,seaturtles
O17
KADAIDALILJUDIIIMIIMOGUDIJELITIISTOSKLONITE?
D.Hamidovi1,V.Lindi2,P.Krstini3
1
Dravnizavodzazatituprirode,Zagreb,Hrvatska(daniela.hamidovic@dzzp.hr)
2
Ministarstvozatiteokoliaiprirode,Upravazainspekcijskeposlove,Zagreb,Hrvatska
3
Javna ustanova za upravljanje zatienim dijelovima prirode Primorskogoranske upanije
"Priroda",Rijeka,Hrvatska
12.HBK2412 CBC
sklonitima s posenim osvrtom na kulturnu batinu. Manje vrste imia roda Pipistrellus
obitavajuuitavomnizusklonitavezanimuzljudskenastambeuzuvjetdajeotvorkrozkoji
moguproiiriod5mm.ZarazlikuodnjihvrsterodaRhinolophusbirajunaputeneobjekte
ili tavanske prostore koji se ne koriste ili se koriste rijetko. Od 2009. godine provedeni su
uviajinatemeljudojavegraanaveimdijelomusredinjojHrvatskoj.Zabiljeenisutipovi,
veliinaistaroststambenihobjekata,nainkoritenjaivrsteimiaukolikojebilomogue
premapredvienomProtokolu.Natemeljurazliitihtipovazahtjevastanarausmisluobnove
objekta ili problema zbog suivota predstavljena su mogua rjeenja u vidu postavljanja
kuica za imie, potrebna zakonska procedura te uspjenost pozitivnih rjeenja.
Predstavljeni su i mehanizmi ouvanja i zatite imia u Starom gradu Bosiljevo kroz
praenjestanjaiograniavanjekonzervatorskihradovanarestauracijidvorca.
Kljunerijei:imii,nadzemnasklonita,sinantropija,EUROBATS,mjereublaavanja
WHENANDIFHUMANSANDBATSMAYSHARETHESAMEROOST
D.Hamidovi1,V.Lindi2,P.Krstini3
1
StateInstituteforNatureProtection,Zagreb,Croatia(daniela.hamidovic@dzzp.hr)
2
Ministry of Environmental and Nature Protection, Directorate for inspectional affairs,
Zagreb,Croatia
3
PublicInstitutionforManagingProtectedNatureAreasCountyofPrimorje&GorskiKotar
"Priroda",Rijeka,Croatia
In this work, importance of cooperation on citizen, expert, legislative and regional level in
conservation and protection of strictly protected bat species is highlighted. Certain bat
species adapted to the cohabitation with humans due to roost loss primarily in forests.
Resolution 5.7 of UNEP/EUROBATS Agreement gives Guidelines for the Protection of
Overground Roosts, with Particular Reference to Roosts in Buildings of Cultural Heritage.
Smaller bat species of the genus Pipistrellus may find roosts that has opening more than 5
mm. Some other species of the genus Rhinolophus prefer abandoned houses or attics that
arerarelyused.Investigationbasedonthecitizencallswereconductedsince2009mostlyin
central part of Croatia. According to the proposed Protocol object type, age and usage
togetherwithbatspecieswererecorded.Takingintoaccountdifferentdemandsofbuilding
owners like renovation or cohabitation conflict, different solutions in terms of bat box
installation, the legislation procedure needed and success of positive solutions are
presented. Conservation and protection mechanisms during restauration of Old Town
Bosiljevothroughmonitoringandlimitationofrenovationworksaregivenasanexample.
Keywords:bats,overgroundroosts,sinantropy,EUROBATS,mitigation
2.Simpozijedukacijebiologije
2ndBiologyEducationSymposium
O18
SIMPLIFIEDIDENTIFICATIONKEYSFORORGANISMS
B.Bajd
FacultyofEducationinLjubljana,Ljubljana,Slovenia(barbara.bajd@guest.arnes.si)
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12.HBK2512 CBC
Simplified identification keys for organisms are important for many reasons. Using such keys
and observing the details, we could learn how to find similarities and differences between
organisms. We thus improve our powers of observation, which is nowadays quite often
replaced by slipshod neglect. Furthermore, we can familiarise ourselves with the exceptional
diversityofspeciesandactuallylearnafewnames.Wealsolearnthebasicstructureofakey.
Professionalidentificationkeysforplantsandanimals,usedbybiologists,areverycomplexand
practically useless for laymen. They include numerous technical terms and too much
informationtheydemandagreatdealofpriorknowledgefromthereader.Usingsimplified
identification keys, we can learn to classify plants and animals, which is one of the basic
activities of natural sciences. And this encourages our thirst for knowledge, since we often
wanttofindoutmoreinterestingfactsaboutthevariousorganisms,whichwecouldlookup
laterinotherbooksorontheinternet.Ifwefindtherightsolutionthenameoftheanimalor
plantwereusuallydelightedandencouragedtoresearchfurther.Theknowledgeobtained
byobservationwithasimplifiedkeythusbecomesmorepermanent.However,wemustkeep
inmindthatthissimplekeywillnothelpusidentifyallorganismsbutonlyalimitednumberof
them.Usingsimplifiedkey,welearnhowkeysarestructuredandhowtoproperlyreadthem,
whilewefamiliariseourselveswithcharacteristicsofanimalsandplantsandtheiradaptations
totheenvironmentinwhichtheylive.
Keywords:primaryscienceeducation,biologyeducation,identificationkeysfororganisms,fieldworkin
nature
O19
AFINITETUENIKAZAIZBORZANIMANJAUPODRUJUBIOLOGIJE
D.Garai1,I.Radanovi2,.Luka3,M.SertiPeri2
1
Agencijazaodgojiobrazovanje,Zagreb,Hrvatska(diana.garasic@gmail.com)
2
Biolokiodsjek,PMFSveuilitauZagrebu,Zagreb,Hrvatska
3
GimnazijaJosipaSlavenskogakovec,akovec,Hrvatska
Istraivanajepovezanostuenikogopredeljenjazazanimanjauznanstvenompodrujubiologije,
siskazaniminteresimaiivotnimiskustvom.Istraivanjetransverzalnogtipaprovedenojetijekom
2010.godinenauzorku2114uenikaod10do18godinastarosti.Afirmativniuenikiodgovori
za tematsko podruje Razvoj i dosezi znanosti, sa srednjom vrijednou 2,8 uzeti su kao
pokazateljistraivakeznatielje.Utvrenojekakoseuenici,sinteresomzaznanosturazliitim
dobnimskupinamaodnoseprema8faktorainteresa,relevantnihzaizborzanimanjaupodruju
biologije ili u srodnim znanostima. Najvee su razlike interesa u korist bioloki zainteresiranih
uenika za faktore Uvjetovanost nasljeem i okoliem te za Reakcije na fizikalne imbenike.
Biolokizainteresiraniuenicisuselektivnijiodukupnoguzorkaispitanika,aikodnjihinteresza
predmet biologiju opada s godinama. Izrazita je povezanost izbora profesije u znanstvenom
podruju biologije i afiniteta za predmet Priroda/Biologija (r = 0,87), koju prati i vrlo visoka
povezanost variranja iskaza uenika (c = 0,89). Uenici koji se ele baviti nekim oblikom
znanstvenogradaubiologijivisokovrednujuKorisnostpredmeta(r=0,56;c=0,45)ipotvrujuu
svojemiskustvueeneposrednekontaktesprirodom(r=0,44;c=0,39).
Kljunerijei:uenikiinteresi,predmetbiologija,izborzanimanja,biolokaznanost
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12.HBK2612 CBC
STUDENTS'AFFINITIESFORPROFESSIONALCARRIERINTHEFIELDOFBIOLOGY
D.Garai1,I.Radanovi2,.Luka3,M.SertiPeri2
1
Education&TeacherTrainingAgency,Zagreb,Croatia(diana.garasic@gmail.com)
2
FacultyofScience,DivisionofBiology,ZagrebUniversity,Zagreb,Croatia
3
HighSchoolJosipaSlavenskogakovec,akovec,Croatia
We investigated the correlation of students' opting for occupations in the scientific field of
biology, with the interest shown and their life experiences. Transversal study was conducted
during 2010 on a sample of 2,114 students from 10 to 18 years of age. Affirmative students'
responsestothethematicareaofDevelopmentandachievementsofscience,withamean2.8
were taken as an indicator of research curiosity. Compared were the answers given by the
different age groups of students interested in science concerning 8 factors, relevant to
occupationalchoiceinthefieldofbiologyorrelatedsciences.Thebiggestdifferencesinfavourof
theinterestedstudentswereshownfortopicsdealingwiththeheritageandtheenvironment,as
well as for biological reactions to physical factors. Biologically interested students were more
selective in their answers than the total sample. Biologically interested students also show the
drop of interest for school subject biology as they are older. Distinct connection between
professional choice for scientific work in the field of biology and a distinctive affinity for the
subject Biology (r = 0.87) were found. Students who wish to engage in some form of scientific
researchinbiologyhighlyvaluetheusefulnessofbiologyasschoolsubjects(r=0.56,c=0.45),
andconfirmedmorefrequentdirectcontactswithnature(r=0.44,c=0.39).
Keywords:students'interests,Biologyasschoolsubject,carrierchoice,biologicalscientificarea
O20
UTJECAJAKTIVNOSTIUPOZNAVANJABILJAKANAMOTIVACIJUIZNANJEUENIKA
.Luka,T.alamon,T.DragiRunjak,N.Sinkovi
GimnazijaJosipaSlavenskogakovec,akovec,Hrvatska(salamon.tanja@gmail.com)
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12.HBK2712 CBC
Kljunerijei:aktivnouenje,izbor,mapabiljaka,motivacija
.Luka,T.alamon,T.DragiRunjak,N.Sinkovi
HighSchoolJosipaSlavenskogakovec,akovec,Croatia(salamon.tanja@gmail.com)
Thestudystartsfromthehypothesisthatapossibilityofchoiceandexpressionofcreativityinthe
activities of familiarizing students with plants through creating a herbarium will affect the
motivation of students, and their attitudes about plants and contribute to a more lasting
acquisitionofknowledge.Thestudyincluded120studentsof2nd,3rdand4thgradeofhighschool,
duringthe2014/2015Thestudents2thgradeweresurveyedpriortotheimplementationofthe
task,andothersafterhavingcreatedherbariaofplants.Thestudentshadtocompleteaherbarium
accordingtotheirchoiceduringtheirsummerholidays,andcouldusedifferenttechniquesortheir
combinations, such as photoherbarium, drawings of plants, pressed plants, etc. and added
additional information with every plant in the herbarium. We analyzed the students herbaria,
tested their knowled ge and surveyed their opinions on the performed activities and their own
progressthroughthem.Studentsgrade3and4showsignificantlybetterresultsthanstudents1st
and2ndgrade.Even43%ofstudentsarebettermotivatedtoworkbecauseofthechoiceofaway
of process plants. Over 66% of students after completing the task recognizes the plants in their
environment. Results showed that despite the initial displeasure task students observed their
progress and that after the task was seen more plants in the environment and in favor of the
hypothesesabouttheneedtousedifferentformsofworkinteachingthatencourageactivityand
student motivation for permanent learning. The results collected through interviews show that
despiteinitialdispleasurewiththeassignedtask,thestudents recognizetheirprogressandthat
afterthetaskhasbeencarriedouttheynoticemoreplantsintheirenvironment,whichsupports
thehypothesisabouttheneedtousedifferentformsofworkinteachingwhichencourageactivity
andmotivationofstudentsforpermanentacquisitionofknowledge.
Keywords:activelearning,choice,herbarium,motivation
O21
MOTIVACIJAUENIKASEDMIHRAZREDAZASTJECANJEZNANJAIZNASTAVNOGPREDMETA
BIOLOGIJA
D.Novoseli1,N.Kleteki2,M.Buli3
1
Alfad.d.,Zagreb,Hrvatska(daniela.novoselic@alfa.hr)
2
OBogumilaTonija,Samobor,Hrvatska
3
OPujanki,Split,Hrvatska
Ciljistraivanjabiojekoritenjem"Upitnikaomotiviranostizauenjebiologije"utvrditijesuli
uenici sedmihrazredamotiviranizauenjebiologije. Uzoraksuiniliuenicisedmihrazreda
jedne gradske osnovne kole. Temeljni instrument bio je anketni upitnik koji sadri trideset
esticazatvorenogtipanakojasuodgovorimoguiunutar5stupanjskeskaleLikertovogtipa.
est je elemenata unutar kojih su rasporeena pitanja upitnika: intrinzina motivacija,
ekstrinzina motivacija, osobna procjena vanosti uenja biologije, lokus unutarnje kontrole,
procjenavlastiteuspjenosti,anksioznostpriprovjeriznanjaizbiologije.Ispitanicisuponekad
doestomotiviranizauenjebiologije.Ekstrinzinosumotivirani:vanaimjedobraocjenaiz
biologijenodotakveocjeneeledoiuzpovremenouenje.Neuoavajupovezanostizmeu
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12.HBK2812 CBC
SEVENTHGRADERSMOTIVATIONFORKNOWLEDGEACQUISITIONOFELEMENTARYSCHOOL
BIOLOGY
D.Novoseli1,N.Kleteki2,M.Buli3
1
Alfad.d.,Zagreb,Croatia(daniela.novoselic@alfa.hr)
2
BogumilToniElementarySchool,Samobor,Croatia
3
PujankeElementarySchool,Split,Croatia
Theaimofthisresearchistodeterminewhethereightgradestudentsaremotivatedtolearn
biology by using the "Motivation to Learn Biology Questionnaire". The sample consists of
seventhgradestudentsfromanurbanelementaryschool.Themainresearchinstrumentisa
questionnaire containing 30 closeended items with a fivepoint Likerttype scale. Items are
distributed between six different components: intrinsic motivation, extrinsic motivation,
personal relevance of learning biology, selfdetermination, personal success assessment, and
anxietyaboutbiologyassessment.Themajorityofparticipantsstatedtheyare"sometimesto
often" motivated to learn biology. They do not consider it a challenge. They feel successful
when they understand the content of the subject. They are extrinsically motivated: a good
gradeinbiologyisimportanttothembuttheywanttoachieveitbylearningoccasionally.They
do not see the connection between the content of biology as a school subject and everyday
life.Studentscareaboutbeingsuccessful,butatthesametime,theydreadfailure.Students
perceivethecauseoftheirsuccessand/orfailureasbeingmoreorlessundertheircontrol.
Key words: motivation, biology, extrinsic motivation, intrinsic motivation, feeling of success, fear of
failure
O22
EBIOLOGIJANAZDRAVSTVENOMVELEUILITU
A.Mojsoviui1,O.Digula2
1
Katedrazabiologiju,Zdravstvenoveleuilite,Zagreb,Hrvatska(ana.mojsoviccuic@zvu.hr)
2
Informatikasluba,Zdravstvenoveleuilite,Zagreb,Hrvatska
Kakobismostudentimaomoguilikvalitetnijesavladavanjegradivaiolakalinastavniproces,na
Zdravstvenom veleuilitu osmislili smo jedan hibridni nastavni proces koji uz klasine naine
pouavanjaukljuujeinaprednetehnolokeistupke.Takosmopredavanjaiseminaresmjestilina
platformu za euenje Moodle te na studentsku platformu za razmjenu datoteka baziranu na
Microsoft sharepoint tehnologiji. Studentima smo omoguili konstantan pristup nastavnim
materijalima,jednostavnijupredajuseminarskihradovateostvarilijednostavnijuibrusuradnju
sa studentima.U vjebovnoj nastavi koritenje samo jednog tipa nastavnog pomagala esto
ograniavamogunostizravnogtumaenjaodreenoggradiva,naroitokadajerijeostaninoj
biologiji. Svjetlosnim mikroskopom, kao osnovnim pomagalom u vjebovnoj nastavi stanine
biologije, ne moemo vidjeti stanine organele u citoplazmi eukariotskih organizama. U tom
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12.HBK2912 CBC
sluaju, osim izrade mikroskopskih preparata, koristimo i aplikacije na tabletima, poput "Cell
World","Cellandcellstructure","Cytogenetics"i"GeneScreen",kojekaoiusluajumikroskopa,
svakistudentdobivanakoritenjezavrijemenastave.Takoer,jedanodmikroskopajeopremljen
kamerom i povezan sa svim tablet raunalima.Treba naglasiti da niti jedno od navedenih
pomagala nije zamjena za ono drugo, ve iskljuivo nadopuna i tek njihovom kombinacijom,
odnosnozajednikomprimjenom,dobivamocjelovitipristupuprocesupouavanja.
Kljunerijei:nastavniproces,biologija,mikroskop,tablet,aplikacije
EBIOLOGYATTHEUNIVERSITYOFAPPLIEDHEALTHSCIENCES
A.Mojsoviui1,O.Digula2
1
Department of Biology, University of Applied Health Sciences, Zagreb, Croatia (ana.mojsovic
cuic@zvu.hr)
2
ITDepartment,UniversityofAppliedHealthSciences,Zagreb,Croatia
Inordertoenablethestudentstomasterthesubjectmatterwithmorequalityandinorderto
ease the teaching process, we have conceived a hybrid teaching process, which in addition to
classic ways of teaching also includes advanced technological approaches. So we installed the
teachingmaterialsonanestudyplatformMoodleandonthestudentplatformforfileexchange,
based on Microsoft sharepoint technology. We have enabled the students to have constant
accesstoteachingmaterial,simplerhandoverofseminarpapersandwehaveaccomplisheda
simpler and faster collaboration with students.In the practical lessons, the use of only one
teaching tool often limits the possibility of direct interpretation of a certain subject topic,
especiallyincellularbiology.Byusingthelightmicroscope,asabasictoolinpracticallessons,we
cannot see cellular organelles in the cytoplasm of eukaryotic organisms. In that case, besides
makingmicroscopicpreparations,wealsouseapplicationsontablets,suchas"CellWorld","Cell
and cell structure", "Cytogenetics" and "Gene Screen", which are given to each student during
classes,thesameasinthecaseofmicroscopes.Also,oneofthemicroscopesisequippedwitha
cameraandconnectedtoalltablets.Itshouldbeemphasizedthatnoneofthementionedtoolsis
areplacementfortheother,butexclusivelyasupplementandweattainacompleteapproachto
theteachingprocessonlythroughtheirmutualapplication.
Keywords:teachingprocess,biology,microscope,tablet,applications
O23
UPORABADIGITALNIHNASTAVNIHSADRAJAUNASTAVIBIOLOGIJE
A.Belani
OsnovnakolaIvaneBrliMaurani,Ogulin,Hrvatska(anita.belancic@skole.hr)
12.HBK3012 CBC
koriste gotove materija le izdavakih kua, a neto manji broj onih koji materijale nalaze na
Internetuiliizraujusami.SkupinformacijaisadrajadostupanputemInternetapotencijalno
znaajnomijenjanainradauiteljaudananjevrijeme.Nainnjihovakoritenjaiprilagodba
predvienom nastavnim sadrajima je zahtjevan, a posljedino prespor zadatak za iru
zajednicunastavnikabiologije.Usvrhuboljeefikasnosti,aiubrzanjaovogprocesa,potrebnoje
raditinarazmjeniiskustava,dijeljenjudigitalnihsadrajaiformalnojedukacijiuiteljauokviru
idejeocjeloivotnomuenju.
Kljunerijei:digitalninastavnisadraj,biologija,osnovnakola
THEUSEOFDIGITALEDUCATIONALCONTENTINTEACHINGBIOLOGY
A.Belani
IvanaBrliMauraniPrimarySchool,Ogulin,Croatia(anita.belancic@skole.hr)
The aim of the research is to analyze the impact of Information and Communication
Technologies(ICT)inteachingBiologyinCroatianelementaryschools,withanemphasisonthe
useofdigitaleducationalcontent.Onlinequestionnairewasusedandresponsesofatotalof
60teachersofbiologywereobtained.TheobtaineddataconfirmthesignificantimpactofICT
on the modern teaching methods. Projects which provide financial support for the school in
theprocurement of IT equipment haveaffected labor conditions70%ofrespondents.Nearly
halfofrespondentsparticipatedinsomeoftheschoolprojectsinvolvingtheuseofICT.Onthis
subjectteacherslearnindependentlyandtheyemphasizethelackofformaleducation.65%of
teachersstatethattheyusedigitaleducationalcontentintheirdailywork,butthereismoreof
thosewhousethereadymadematerials,andaslightlysmallernumberofthosewhosearch
digitalcontentontheInternetordevelopitbythemselves.Thesetofinformationandcontent
available on the Internet potentially significantly changes nowadays teachers methods. The
way of their use and adaptation of the planned teaching content is demanding, and
consequently slow task for the wider community of teachers. To improve the efficiency and
accelerationofthisprocess,itisnecessarytoworkontheexchangeofexperiences,sharingof
digitalcontentandtheformaleducationofteachersintheframeworkoftheideaoflifelong
learning.
Keywords:digitaleducationalcontent,biology,primaryschool
O24
PROJEKTNATERENSKANASTAVANAOBRONCIMAMEDVEDNICE
L.ari1,M.Varga2
1
OsnovnakolaRemete,Zagreb,Hrvatska(lana.saric2712@gmail.com)
2
OsnovnakolaRetkovec,Zagreb,Hrvatska
Terenska nastava kao oblik uionike nastave pedagoki je uinkovita i zanimljiva praksa.
Nastavnisadrajipredoenikaoprimarniizvorznanjapostajuvidljiviiopipljivi,asamimtimei
zanimljiviji, to uzrokuje njihovo lake pamenje.Okoli Medvednice prua mnogobrojne
mogunosti za otkrivanje i razumijevanje svijeta u kojem jesmo. Cilj je projekta motivirati i
osvijestiti uenike o vanosti ouvanja prirodne batine. Projekt je zamiljen kao viegodinji
(zapoetusvibnju2014.godine)krozkojiseuenicimaukazujenapromjeneuvodenomsvijetu
nastaledjelovanjemprirodeiovjeka.Uenicisunosiocisvihaktivnostiusvetrietapeterenske
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nastave pri emu je naglasak stavljen na praktian rad na terenu odreivanje razliitih
parametara vode metodama i nainima prilagoenim kognitivnim i psihomotorikim
sposobnostima dane uenike dobi. Sve se etape izvode metodom grupnog rada
polustrukturiranim zadacima koji obuhvaaju kemijsku analizu vode potoka, odnosno
odreivanje kloridnih, fosfatnih, nitratnih, nitritnih te amonijevih iona u vodi, tvrdoe, pH i
temperature vode, slobodnog i vezanog CO2, brzine protoka te YTTtest.Projektna terenska
nastava kao sloeni oblik praktinog, misaonog i neposrednog stjecanja znanja potie kod
uenikaintelektualnuradoznalost,toutjeenameuvrnjaku(znanstvenu)komunikacijute
stvaranjekvalitetnihodnosameusamimuenicimateizmeunastavnikaiuenika.
Kljunerijei:projektnanastava,terenskanastava,motivacija,zatitaprirode
PROJECTFIELDCLASSESONTHESLOPESOFMEDVEDNICA
L.ari1,M.Varga2
1
PrimarySchoolRemete,Zagreb,Croatia(lana.saric2712@gmail.com)
2
PrimarySchoolRetkovec,Zagreb,Croatia
Fieldclassesasaformofteachingprocessareaneducationallyeffectiveandinterestingpractice.
Thecurriculacontentpresentedasaprimaryknowledgesourcebecomevisibleandtangible,and
in turn more interesting, which makes them easily memorable.The natural resources of
MedvednicaHillofferavarietyofpossibilitiesfordiscoveringandunderstandingtheworldwelive
in.Theobjectiveoftheprojectistomotivatestudentsandtomakethemawareoftheimportance
ofconservationofnaturalheritage.TheprojectstartedinMayof2014anditisenvisionedasa
severalyearlongproductiveprojectthroughwhichstudentsdiscoverthechangesintheaquatic
world that have occurred due to natural and mancaused effects.Students are the main
contributorsintheactivitiesandthestressisonpracticalwork,i.e.determiningvariousproperties
and parameters of creek water methods tailored to the students age in the cognitive and
psychomotor sense. All the work stages are implemented by means of group work on semi
structuredtaskscomprisingachemicalandphysicalanalysisofthecreekwater.Theprojectfield
classes, as a complex practical, cognitive and direct knowledge acquisition process, arouse the
intellectual curiosity in students, which in turn influences not only the quality of intercoeval
(scientific)communication,butalsoenhancesrelationshipsandcamaraderieamongthestudents
themselves.
Keywords:projectstrategies,fieldclasses,motivation,environmentalprotection
O25
ANALIZA STAVOVA I INTERESA UENIKA SREDNJIH KOLA O PRIRODNIM VRIJEDNOSTIMA
MEIMURSKEUPANIJE
M.Cindri1,D.Crnec2,M.Mesari3
1
Srednjakolaakovec,akovec,Hrvatska(monica.pbk@gmail.com)
2
OSv.MartinnaMuri,Sv.MartinnaMuri
3
MeimurskaprirodaJavnaustanovazazatituprirode,MurskoSredie
Zadaa ekoloke edukacije je prenijeti poruku o znaaju i nunosti ouvanja prirodnih i drugih
vrijednosti, a najmonijim sredstvom ekoloke edukacije smatra se interpretacija. Ve je otac
interpretacije Freeman Tilden istaknuo: "Interpretacijom do razumijevanja, razumijevanjem do
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Kljunerijei:prirodoslovlje,prirodnevrijednosti,uvariprirode,stavovi
MIDDLE SCHOOL STUDENTS ATTITUDES AND INTERES ANALYSIS ABOUT THEIR NATURAL
VALUESINTHEMEIMURJECOUNTY
M.Cindri1,D.Crnec2,M.Mesari3
1
HighSchoolakovec,akovec,Croatia(monica.pbk@gmail.com)
2
PrimaryschoolSv.MartinnaMuri,Sv.MartinnaMuri,Croatia
3
MeimurjeNaturePublicInstitutionfortheProtectionofNature,MurskoSredieCroatia
Thetaskofecologicaleducationistoconveyamessageabouttheimportanceandnecessityof
preserving natural and other values, and the most powerful tool for ecological education is
considered to be the interpretation. The father of interpretation, Freeman Tilden said:
Through interpretation, understanding; through understanding, appreciation; through
appreciation, protection. Employees of Meimurska priroda (Meimurje nature), and two
teachersworkedout,usingdirectandindirectformsofinterpretation,aneducationalprogram
calledYoungguardiansofnature.Fourtypupilsfromthesixthgradefromallprimaryschoolsof
theMeimurjecountyattendedtheeducationalprogram.Theprogramwasrealisedthrougha
seriesoflectures,workshopsandfieldworkwhichtookplaceduringthespringof2009witha
goalofecologicaleducationandincreasingpublicinterestfornaturalsciences.Thegoalinthis
paperistodeterminetheinfluenceoftheprogramMladiuvariprirodeonthedevelopmentof
a more positive attitude on the protection of nature in the Meimurje county aswell as
increasing the public interest for natural sciences. Students took an online survey and gave
theiropinion.ParticipantsoftheMladiuvariprirode2009tookthesurvey,whiletheirpeers
werethecontrolgroup,whodidn'tparticipateinthementionedprogram.Exceptviewpoints,
the survey tested interests for natural sciences with special emphasis on protected parts of
nature.
Keywords:naturalscience,naturalvalues,Youngguardiansofnature,attitudes
O26
RAZVOJ JEZINIH, DRUTVENIH I UPRAVLJAKIH KOMPETENCIJA U UENIKIM
ISTRAIVAKIMPROJEKTIMAUPROGRAMUGLOBEIBIOLOGIJI
M.LabaHorvat,M.Raan
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SrednjakolaPrelog,Prelog,Hrvatska(rasanmiso@gmail.com)
SrednjakolaPrelogje12godinaukljuenauGLOBEprogramiuokviruGLOBEaiizbornenastave
biologije odraeni su brojni ueniki istraivaki projekti vezani uz prirodne ekosustave u
okruenju(jezero,rijeka,travnjak,uma)kojeueniciradeuparuiligrupiod3uenika.Zanjihovu
su izradu potrebne razliite komunikacijske, socijalne i druge kompetencije. Uenici pri
prouavanju uoenog problema i odabiru metoda za rjeavanje istraivakog pitanja koriste
literaturunaengleskomjeziku.Ciljistraivanjajepronaiodgovarajuealatezamjerenjejezinihi
upravljakih vjetina prije i nakon izrade projekta te odabranim alatima mjeriti drutvene
kompetencijeurazliitimfazamaizradeprojektaispitivaneskupineuenika(kojiradeistraivake
radove)uodnosunakontrolnuskupinu(kojineradeistraivakeradove).Pretpostavljasedae
ispitivana skupina mjerenjem navedenih kompetencija tijekom istraivanja pokazati bolje
rezultate. Za mjerenje jezinih kompetencija su koritene CEFR tablice te upitnici za ispitivanje
drutvenih i upravljakih kompetencija. Rezultati istraivanja su sljedei: uenici ukljueni u
projekte su pokazali izniman napredak unutar CEFR tablice, drutvene vjetine su uvelike
poboljane,uenicisunauilidijelitizaduenjatesurazvilibrojneupravljakevjetine,kaotosu
prezentacija,javnogovorenje,donoenjeodlukaipregovaranje.
Kljunerijei:jezine,drutveneiupravljakekompetencije
M.LabaHorvat,M.Raan
HighSchoolPrelog,Prelog,Croatia(rasanmiso@gmail.com)
Secondary school Prelog has been involved in GLOBE for 12 years and a number of student
researchprojectsconnectedtonearbynaturalecosystemshavebeendonebystudentsinpairs
or groups of three. In order to work on the project, students need various communicative,
social and other competencies. While researching the noticed problem and deciding on the
methods to solve the research question, students search through the English literature. The
goal of the research is to find adequate tools to measure linguistic and management skills
beforeandaftertheproject,aswellastomeasuresocialcompetenciesbyusingchosentools
in different stages of the project of the questioned group of students (working on projects)
comparedtothecontolledgroup(notworkingonprojects).Itiisassumedthatthequestioned
groupwillshowbetterresultsofthemeasuredcompetenciesduringtheresearch.CEFRgrids
were used to measure linguistic competencies, and questionaires for measuring social and
managementcompetencies.Theresultsoftheresearchare:studentsinvolvedinprojectshave
shownamazingprogresswithinCEFRgrid,theirsocialskillswerealsoenhancedandtheyhave
learnedtoshareresponsibilitiesandhavealsodevelopedvariousmanagementskills,suchas
presentation,publicspeaking,decisionmakingandnegotiating.
Keywords:linguistic,socialandmenagementskills
O27
PREDSTAVLJANJE REZULTATA POTPROJEKATA ISTRAIVANJE I REKREACIJA U PRIRODI I
NASTAVNE METODE U BIOLOGIJI I KEMIJI U OKVIRU PROJEKTA AFIRMATIVNA NASTAVA I
INOVATIVNO UENJE I POUAVANJE U GIMNAZIJAMA U OKVIRU HRVATSKOG
KVALIFIKACIJSKOGOKVIRAIPAQPETA
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12.HBK3412 CBC
Z.Pongractimac,R.HalapirFrankovi
V.gimnazija,Zagreb,Hrvatska(zrinkaps@gmail.com)
V.gimnazijajebilanositeljprojektaIPAQPeta,kojisuosmislilinjeniprofesori,aprovedenjeu
suradnjispartnerima:PMFom,kolamaizVukovara,Metkovia,PakracaiKninatesuradnikom,
AZOO. Kroz sedam potprojekata, namijenjenih nastavnicima, eljeli smo razviti i modernizirati
obrazovnisustavikvalifikacijeugimnazijama;moderniziratipostojeeirazvitinaprednekurikule
zagimnazijetemeljenenaishodimauenjairazvitinastavnekapacitetezaprovedbuinovativnog
uenja i pouavanja. Potprojektom Istraivanje i rekreacija u prirodi izradili smo kurikul koji
sadrajima obuhvaa biologiju, kemiju, geologiju i tjelesnu i zdravstvenu kulturu. Nekoliko
znanstvenih metoda istraivanja u prirodi prilagoeno je uenicima i nastavnicima te je
osmiljena kvalitetna rekreacija u prirodi. Potprojektom Nastavne metode u biologiji i kemiji
izradilismokurikulkojisedijelomnadovezujenaterenskunastavu,adijelomvepoznatemetode
usmjeravanauenikainastojiostvaritipozitivaniodgovoranstavpremazdravlju,ivomsvijetu,
prirodi i znanosti. Najvaniji cilj ovih potprojekata je promijeniti poloaj uenika i poloaj
nastavnika u nastavnom procesu. Nastavnikovu ulogu predavaa elimo promijeniti u ulogu
organizatora, menadera, mentora, instruktora i suradnika, a uenike elimo iz pasivnih
promatraa promijeniti u angairane sudionike nastave, osamostaliti ih u uenju, dodatno ih
motiviratiipoticatinasuradnjuipreuzimanjeodreeneodgovornosti.
Kljunerijei:kurikul,ishodiuenja,Hrvatskikvalifikacijskiokvir
Z.Pongractimac,R.HalapirFrankovi
FifthGymnasium,Zagreb,Croatia(zrinkaps@gmail.com)
FifthGymnasiumwastheprojectleaderofIPAQPeta,whichwasconceivedbyitsprofessors
and implemented in cooperation with the Faculty of Science, four schools from Vukovar,
Metkovi,PakracandKninandAZOO(EducationandTeacherTrainingAgency).Inourproject,
consisting of 7 subprojects, we wanted to develop and modernize the teaching system and
advance qualifications in grammar schools; modernize the existing and develop advanced
curricula for grammar schools based on the proper use of learning outcomes, and develop
teachingcapacitiesfortheimplementationofstudentcentredlearning.Withinthesubproject
Research and recreation in nature, we have created a curriculum that includes the fields of
biology, chemistry, geology and physical education. Several scientific research methods in
natureareadaptedtostudentsandteachersanditalsocontainsqualityrecreationinnature.
Within the subproject Teaching methods in biology and chemistry, we have created a
curriculum consisting of wellknown methods directed at students and aiming to achieve a
positive and responsible attitude towards health, the living world, nature and science. The
mostimportantobjectiveofthissubprojectistochangethepositionofthestudentsandthe
roleofteachersin theteachingprocess.Wewantto transformtheteachers'roleoftrainers
into the role of organizers, managers, mentors, instructors and assistants, and the students'
role of passive observers into engaged participants, teaching them to become independent
learnersandfurthermotivateandencouragethemtocooperateandtakesomeresponsibility.
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Keywords:curriculum,learningoutcomes,CroatianQualificationsFramework
O28
PRIMJER INTEGRIRANJA RAZLIITIH NASTAVNIH PREDMETA NA TEMU ZATITE LOKALITETA
BEDEKOVIEVEGRABE
.Kadi1,J.Pavlic2
1
Ekonomskaitrgovakakolaakovec,akovec,Hrvatska(zeljka.kadi@gmail.com)
2
OsnovnakolaIvanaGoranaKovaiaSvetiJurajnaBregu,Lopatinec,Hrvatska
.Kadi1,J.Pavlic2
1
SecondarySchoolofEconomicsandTradeakovec,akovec,Croatia(zeljka.kadi@gmail.com)
2
PrimarySchoolIvanGoranKovaiSv.JurajnaBregu,Lopatinec,Croatia
Basedontheresultsoftheresearchintothebiologicalfeaturesofthebutterfliesofthespecies
Maculinea at the site "Bedekovieve grabe" in Meimurje many long lasting educational
activitieshavebeendevisedandimplementedwiththeaimofpreservationandprotectionof
thehabitatofthescarcelargebluebutterflyMaculineateleiusBrgstr.andtheduskylargeblue
butterfly Maculinea nausithous Brgst. The aim of the activities was to make students of
different age and educational sector more aware of the need to preserve endangered and
protected large blue butterflies in Meimurje by their active involvement in interdisciplinary
projects.Theresultsachievedweretheintegrationofvariousschoolsubjectsinprimaryschool
(grade1tograde8)andsecondaryschoolstudents'projectswhichdependedontheirareaof
interest:ecology,economics,trade,tourismandhotelindustry.Theparticipantsinvolvedwere
studentsandteachersfromPrimarySchoolIvanGoranKovai,Sv.JurajnaBregu;Secondary
School of Economics and Trade, akovec; Secondary School Prelog; Grammar School J.
Slavenskog akovec and Vocational School akovec. Using the results of the implemented
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activities, the second part of the study focuses on conducting the sample survey into the
studentsattitudesandcompetence,particularlyentrepreneurialandscientificcompetence.
O29
ISTRAIVANJESTAVOVAUENIKAOPREHRANI
N.Graovac,E.Merdi
Odjel za biologiju, Sveuilite Josipa Jurja Strossmayera, Osijek, Hrvatska
(enrih@biologija.unios.hr)
Osnovniciljovogaradabiojesaznatiprehrambeneidrugenavike,stavoveiaktivnostiuenika,
te utvrditi postoje li razlike meu uenicima ovisno o spolu, dobi i sredini u kojoj ive.
Istraivanje je provedeno putem upitnika konstruiranog za potrebe ovog istraivanja. U
istraivanju je sudjelovao 651 uenik. Najvei broj uenika je normalne tjelesne mase, 481
(73,88%). Uhranjenost djeaka i djevojica se razlikuje. Uenici ruralne sredine dorukuju
redovitije(p=0,010),eejeduperad(p=0,008)tedomaesuhomesnateproizvode(p=0,003),
dokueniciurbanesredineeejeduribu(p=0,044)icjeloviteitarice(p<0,001).Odrastanjem
uenika poveava se neredovitost doruka (p=0,022), konzumacija slastica (p=0,009) te
gaziranih sokova (p=0,027). Briga uenika o tjelesnom izgledu poveava se odrastanjem
(p=0,048), to je vie izraeno kod djevojica (p<0,001). Konzumacija alkohola (p=0,009) i
cigareta (p=0,045) ea je kod uenika ruralne sredine. Djeaci su tjelesno aktivniji od
djevojica (p<0,001). Uenici viih razreda skloniji su sedentarnim aktivnostima (p<0,001).
Ispitaniuenicisuuglavnomnormalnetjelesnemasezasvojudob,audiopothranjenihipretilih
uenika vei je kod djeaka. Prehrambene navike razlikuju se izmeu uenika iz urbane i
ruralnesredineinerazlikujuseznaajnoizmeudjeakaidjevojica,aporastomdobipostaju
loije. Briga uenika o tjelesnom izgledu poveava se odrastanjem, to je vie izraeno kod
djevojica.
Kljunerijei:uenici,stavuenika,zdravnainivota,adolescencija,upitnik
PUPIL'SDIETRELATEDATTITUDESTOHEALTHYLIFESTYLE
N.Graovac,E.Merdi
Department of Biology, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
(enrih@biologija.unios.hr)
The main purpose of this research was to find out more about eating habits along with other
habits, attitudes and activities of elementary school pupils, as well as to determine possible
differences between pupils, depending on their sex, age and environment. The research was
conductedviaquestionnaireconstructedfortheneedsofthisspecificresearch.651pupilstook
part in this questionnaire. Most of the pupils had normal body mass, 481 (73.88%). The
nutritional status of boys and girls differ. Pupils from the rural environment are more keen on
havingbreakfast(p=0.010),theymoreofteneatpoultrymeat(p=0.008)aswellassmokedmeat
(p=0.003).Ontheotherhand,pupilsfromtheurbanenvironmentmoreofteneatfish(p=0.044)
and whole grains (p<0.001). During childhood, breakfast irregularities increase (p=0.022) along
withtheconsumptionofsweets(p=0.009)andsoftdrinks(p=0.027).Pupils'concernaboutbody
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appearanceincreasesastheygrowup(p=0.048),whichismoreexpressedamonggirls(p<0.001).
The consumption of alcohol (p=0.009) and cigarettes (p=0.045) is more common among the
pupils from the rural environment. Most of the interviewed pupils have normal body mass
accordingtotheirage.Theshareofunderweightandoverweightpupilsisbiggeramongtheboys.
Nutritionalhabitsdifferbetweenpupilsfromtheurbanandruralareas,buttheydonotdifferas
muchbetweenboysandgirls.Inaddition,theirnutritionalhabitsbecomeworseastheygrowup.
Keywords:pupils,dietrelatedattitude,healthylifestyle,adolescence,questionnaire
O30
ANATOMYANDPHYSIOLOGYOFHUMANBODYTEACHINGWITHNATURALMATERIALS
A.pernjak
Department of Biology, Faculty of Natural Sciences and Mathematics, University of Maribor,
Maribor,Slovenia(andreja.spernjak@um.si)
Keywords:biologyclass,experiencelearning,freshmammalianorgans
O31
ISTRAIVANJE UENIKOG RAZUMIJEVANJA OSNOVNOG BIOLOKOG KONCEPTA
MENSTRUACIJSKOGCIKLUSA
M.Rui1,I.Podrug2,D.Sirovina3
1
PrirodoslovnomatematikifakultetSplit,SveuiliteuSplitu,Split,Hrvatska(mrus@pmfst.hr)
2
OsnovnakoladonLovreKatiaSolin,Solin,Hrvatska
3
PrirodoslovnomatematikifakultetZagreb,SveuiliteuZagrebu,Zagreb,Hrvatska
Uradujeistraenausvojenostirazumijevanjekonceptamenstruacijskogciklusa,kaojednogod
najsavrenijih i najkompleksnijih biolokih sustava. U istraivanju je sudjelovalo 220 uenika
treih razreda splitskih gimnazija (148 djevojica i 56 djeaka te 16 neopredijeljenih).
Istraivanjejeprovedenoputom4kombinacijetestova,odtogatritestasgrafikimprikazom
razliite duine menstruacijskog ciklusa i jedan test bez grafa. Svaki test je imao 6 pitanja.
Ukupna rijeenost odreivanja datuma ovulacije iznosila je 67,39% i ako se usporedi rezultat
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Kljunerijei:menstruacijskiciklus,koncept,uenik,ovulacija,biologija
M.Rui1,I.Podrug2,D.Sirovina3
1
FacultyofSciencesSplit,UniversityofSplit,Split,Croatia(mrus@pmfst.hr)
2
ElementarySchooldonLovreKatiaSolin,Solin,Croatia
3
FacultyofScience,UniversityofZagreb,Zagreb,Croatia
The paper investigates the process of learning and understanding of the concept of menstrual
cycle,asoneofthemostperfectedandcomplexbiologicalsystems.Theresearchwasconducted
onasampleof220participants,3rdgradestudentsofgrammarschoolsinSplit(148girls,56boys
and16unspecified).Theresearchwascarriedoutusingthecombinationof4tests,outofwhich
three tests included the graphical representation with different lengths of the menstrual cycle,
andonetestdidnotincludegraphicalrepresentation.Eachtestconsistedof6questions.Total
success rate in defining the date of ovulation was 67.39% and when a comparison was made
between the results based on gender, there was no significant difference between the results
achievedbythegirlsandthoseachievedbytheboys.Successrateincompletingtheanswersto
furtherquestionsregardingtheprooffortheselecteddateofovulationwasquitelowaslowas
14.49%,whichindicatesthattheselecteddateofovulationwasrandom.Inthesectionrelatedto
the analysis of the phase with the highest level of female sex hormones, 34.78% of students
answeredcorrectlyandfoundthevaluesinthegraph.Thesectionrelatedtothelowestlevelof
femalesexhormoneswascorrectlyansweredby56.16%ofstudents.Thelinkbetweenthelow
leveloffemalesexhormonesandnewoccurrenceofmenstruationwasidentifiedby68.12%of
students. The summarised results of the research show low levels of comprehension and
understanding of the concept of menstrual cycle, which indicate that the lecturebased teaching
modelandpassiveroleofthestudentsshouldbereplacedbymoremoderncurricularapproachesin
teachingbiology.
Keywords:menstrualcycle,concept,student,ovulation,biology
O32
ANALIZA ZASTUPLJENOSTI RAZLIITIH TIPOVA ZADATAKA I NJIHOVIH KOGNITIVNIH RAZINA
U TESTOVIMA UPANIJSKIH NATJECANJA BIOLOGIJE ZA OSME RAZREDE OD 2009. 2014.
GODINE
D.Crnec
OSvetiMartinnaMuri,SvetiMartinnaMuri,Hrvatska(dcrncec1@gmail.com)
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U organizaciji Ministarstva znanosti, obrazovanja i sporta, Agencije za odgoj i obrazovanje te
Hrvatskog biolokog drutva godinama se provodi natjecanje u znanju iz biologije. Cilj
istraivanjajeanaliziratipisanezadaeupanijskihnatjecanjaizbiologijeuosmomrazreduna
temelju tipova zadataka, njihove zastupljenosti te kognitivne razine pitanja (tri razine prema
pojednostavljenoj Bloomovoj taksonomiji; Crooks).Analiza tipova zadataka i njihove
zastupljenosti provedena na bazi est pisanih zadaa od 2009 do 2014 godine (ukupno 183
zadatka)pokazalajedasenajviekoristezadacizatvorenogtipa.Analizatakoerpokazujeda
su najzastupljeniji zadaci viestrukog izbora, dok se zadaci otvorenog tipa iznimno rijetko
koriste. Kategorizacija zadataka prema kognitivnim razinama pokazuje visoku zastupljenost
zadatakaprverazine,uztendencijupoveanjauporabezadatakadrugeitreekognitivnerazine
tijekomistraivanograzdoblja.Temeljemdobivenihpodatakaukazujesenapotrebusustavnije
i detaljnije analize pisanih zadaa na svim razinama provjeravanja (kolska, upanijska,
dravna), veeg koritenja zadataka otvorenog tipa u pisanim zadaama, te poveanju
zastupljenostizadatakadrugeitreekognitivnerazine.
Kljunerijei:natjecanja,tipovizadataka,kognitivnerazine
ANALYSISOFTHEREPRESENTATIONOFDIFFERENTTYPESOFTASKSANDTHEIRCOGNITIVELEVELS
INCOUNTYLEVELCOMPETITIONTESTSINBIOLOGYFOREIGHTHGRADESFROM2009TO2014
D.Crnec
PrimarySchoolSvetiMartinnaMuri,SvetiMartinnaMuri,Croatia(dcrncec1@gmail.com)
Organized by the Ministry of Science, Education and Sports, Agency for Education and the
CroatianBiologicalSociety,competitionsinbiologyknowledgehavebeenconductedforyears.
Theaimoftheresearchistoanalyzewrittenassignmentsincountylevelbiologycompetitions
intheeighthgradebasedonthetypeoftasks,theirrepresentationandthecognitivelevelof
questions(threelevels,accordingtothesimplifiedBloomstaxonomy;Crooks).Theanalysisof
the type of tasks and their representation conducted based on six written assignments from
2009to2014(183tasksintotal)hasshownthatthemostfrequentlyusedtypeoftasksisthe
closed type. The analysis has also shown that the most represented ones are the multiple
choice tasks, while the open tasks are used extremely rarely. The categorization of tasks
accordingtocognitivelevelsshowsahighrepresentationoffirstleveltasks,withatendencyto
increase the use of second and third cognitive level tasks during the period researched. The
obtained data indicates that there is a need to analyze the written assignments more
systematicallyandingreaterdetailatalllevelsoftesting(school,county,state),tousemore
open tasks in written assignments and to increase the representation of second and third
cognitiveleveltasks.
Keywords:competitions,tasktypes,cognitivelevels
O33
PROCJENAKVALITETEPITANJAIZPRIRODEIDRUTVAZA4.RAZREDO
S.Raan1,M.Raan2
1
OsnovnakolaHodoan,Hodoan,DonjiKraljevec,Hrvatska
2
SrednjakolaPrelog,Prelog,Hrvatska(rasanmiso@gmail.com)
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12.HBK4012 CBC
U nastavi prirode i drutva za 4. razred osnovne kole postoji u uporabi vie odobrenih
udbenika i radnih biljenica. U radnim se biljenicama nude zadatci razliitog tipa. Autori
istraivanja su proveli akcijsko istraivanje koje je temeljeno na procjeni dvoje nastavnika
praktiara. Prema rezultatima akcijskog istraivanja u radnim biljenicama dominiraju pitanja
niekognitivnerazine.Namjerajebilanastavitiistraivanjesvieprocjenjivaaraspodjeljenihu
dvije grupe ispitanika: (I.) uitelji razredne nastave i (II.) nastavnici prirode i biologije u viim
razredimaosnovnihkolaMeimurskeupanije.Ciljjeistraivanjausporeditiprocjenekvalitete
pitanja navedenih ispitanika. Iz svih radnih biljenica zastupljenih na tritu su odabrana 22
pitanja za istraivanje. Ispitanici procjenjuju kvalitetu pitanja u sljedeim kategorijama: (1.)
vanost pitanja za nastavak obrazovanja, (2.) primjena postignua u sadanjem i buduem
ivotu te (3.) kognitivne razine postignua. Ispitanici procjenjuju svako pitanje Likertovom
skalom od pet (1. i 2.) stupnjeva. Kognitivne razine se procjenjuju u tri stupnja. Analizom
rezultatadvijeskupineispitanikaustanovljenajestatistikiznaajnarazlikauocijenjivanju(1.)
vanostipitanjazanastavakobrazovanjai(2.)vanostipitanjazaivot.Veinaispitanikaizobje
skupinesmatrajudanajvieprevladavajupitanjauglavnom1.i2.kognitivnerazine.Pitanja3.
razinenisuzastupljenauradnimbiljenicama.
Kljunerijei:akcijskoistraivanje,kognitivnarazina,Likertovaskala
ESTIMATINGTHEQUALITYOFTESTQUESTIONSFROMNATUREANDSOCIETYINTHEFOURTH
GRADE
S.Raan1,M.Raan2
1
PrimaryschoolHodoan,DonjiKraljevec,Croatia
2
HighschoolPrelog,Prelog,Croatia(rasanmiso@gmail.com)
Keywords:actionresearch,cognitivelevel,Likertscale
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O34
UTJECAJ KVALITETE ZNANJA UENIKA NA RJEAVANJE ZADATAKA VIIH KOGNITIVNIH
RAZINA
V.Begi1,M.Basti2,I.Radanovi3
1
OsnovnakolaSesvetskiKraljevec,SesvetskiKraljevec,Hrvatska(valerija1.begic@gmail.com)
2
OsnovnakolaRude,Zagreb,Hrvatska
3
Katedra za metodiku nastave biologije, Prirodoslovno matematiki fakultet Sveuilita u
Zagrebu,Zagreb,Hrvatska
Ciljistraivanjabiojeispitatikonceptualnorazumijevanjenastavnihsadrajabiologijenauzorku
pisanihzadaauenika 7. razreda sa upanijskerazinenatjecanja.Uzorakje sadravaonajbolje
rijeene pisane zadae po upanijama, odnosno zadae u kojima je tono rijeeno 75%
zadataka.Pisana zadaa sadravala je zadatke oblikovane u skladu s preporukama za pripremu
pisanih provjera iz biologije. Analizom bodova za vrednovanje pojedinih zadataka, udio II.
kognitivnerazineje60%(rasporeenihu14zadataka),aIII.kognitivnerazine18%(rasporeenih
u 2zadatka).Rezultati su obraeni (SPSS 17) u odnosu na definirane ishode uenja u skladu s
makrokonceptualnim okvirom biologije, procjenu kvalitete zadataka (od strane autora i 10
neovisnih osoba iz struke), prilagoenost tipa zadatka ispitivanju odreenog koncepta, utjecaj
kvalitete zadataka na stupanj diskriminacije, usklaenost s nastavnim sadrajima biologije 7.
razredaosnovnekolepropisanihnastavnimplanomiprogramom,povezanostsasvakodnevnim
ivotom, opom kulturom i nastavnim sadrajima drugih prirodoslovnih predmeta. Zadatci
otvorenog tipa uglavnom bolje diskriminiraju uspjenije uenike. Uenici koji na cjelokupnoj
pisanoj zadai postiu vei broj bodova bolje rjeavaju zadatke koji zahtijevaju cjeloviti pristup
rjeavanjuproblemauodnosunazadatkekojiispitujudetalje.
Kljune rijei: kognitivne razine, kvaliteta pitanja, nastavni sadraji biologije, meupredmetni
sadrajiprirodoslovlja
V.Begi1,M.Basti2,I.Radanovi3
1
ElementarySchoolSesvetskiKraljevec,SesvetskiKraljevec,Croatia(valerija1.begic@gmail.com)
2
ElementarySchoolRude,Zagreb,Croatia
3
Division of Methodology for Teachers of Biology, Faculty of Science, University of Zagreb,
Zagreb,Croatia
The aim of this paper was to determine the conceptual understanding of biology curriculum
content on a sample of tests written by 7th grade students in the County competition. The
samplewascomprisedofbestsolvedtestsinthecounties,thatis,testscoresof75%.Thetasks
inthetestwereconsistentwiththerecommendationsfordesigningwrittentestsinbiology.By
analysing points for task evaluation, 60% of the test was aligned for the tasks of the second
cognitivelevel(14tasks)and18%wasalignedforthetasksofthethirdcognitivelevel(2tasks).
Theresults(SPSS17)wereevaluatedinview ofthe definedlearningoutcomes basedonthe
macroconceptual biology framework, the quality of the tasks (evaluated by authors and 10
independentexperts),thesuitabilityofthetypesoftasksandcertainconcepts,theinfluenceof
taskqualityonthedegreeofdiscrimination,thecompliancewiththe7thgradecurriculum,the
correlation with everyday life, general knowledge and the science curriculum in general. In
general,theopenendedtasksmorereliablydiscriminatedsuccessfulstudents.Studentswith
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betteroverallscoreshowbetterresultsinsolvingtasksthatrequireaholisticapproachthan
solvingtasksorientedtoparticulartypeofinformation.
Keywords:cognitivelevels,qualityofthequestion,7thgradebiologycurriculum,sciencecurriculum
O35
USPJENOSTUENIKA8.RAZREDAURJEAVANJUPISANIHZADATAKAIZBIOLOGIJE
L.Lugar1,A.Musta2
1
OPore,Pore,Hrvatska(lydialugar@yahoo.com)
2
OimeBudinia,Zadar,Hrvatska
Kljunerijei:kognitivnerazine,uspjenostrjeavanjazadataka
EFFICIENCYOFTHE8THGRADEPUPILSINSOLVINGWRITTENASSIGNMENTSINBIOLOGY
L.Lugar1,A.Musta2
1
ElementarySchoolPore,Pore,Croatia(lydialugar@yahoo.com)
2
ElementarySchoolimeBudinia,Zadar,Croatia
The aim of this research is to analyse the efficiency of the 8th grade pupils in solving
assignments of different cognitive levels. The research sample consists of 463 pupils who
participated in the County Biology Competition in the category which examines knowledge
withwrittentests,inthe20142015schoolyear.Thetotalnumberofpointsinwrittentestwas
50.Themaximumnumberofpointsachievedwas47,whiletheminimumachievedwas11.The
analysesofthecompetition'sresultsshowedthatthe0.01%ofpupilssolved90%ormoreof
thetestaccurately.The10%ofthemostsuccessfulpupilsthatparticipatedinthecompetition
achievedfrom38.5to47points,i.e.from77%to94%ofthetestwasaccuratelysolved.The
analysesofthepupil'stestsconfirmedourassumptionthatthepupilswhosolved90%ormore
of the test accurately would solve the 2nd and 3rd cognitive level assignments in higher
percentagethanthe10%ofthemostsuccessfulpupilsthatparticipatedinthecompetition.
Keywords:cognitivelevels,theefficiencyofassignmentsolving
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Evolucija,sistematika,filogenijaibiogeografija
Evolution,systematics,phylogenyandbiogeography
O36
EVOLUCIJA U DINARIDIMA ANALIZA FILOGEOGRAFSKE I POPULACIJSKE GENETSKE
STRUKTURERIBARODADelminichthys
I.Buj1,Z.Mari1,M.aleta2,T.Mihinja1,R.anda3,D.Zanella1,P.Mustafi1,M.Mrakovi1
1
Zoologijskizavod,Biolokiodsjek,PMF,Zagreb,Hrvatska(ivaradic@biol.pmf.hr)
2
Uiteljskifakultet,Zagreb,Hrvatska
3
Narodnimuzej,Prag,Republikaeka
Kljune rijei: Dinarski kr, slatkovodni sustavi, populacijska struktura, genetska raznolikost,
protokgena
EVOLUTIONINDINARIDSINTEGRATIVEANALYSISOFPHYLOGEOGRAPHYANDPOPULATION
GENETICSTRUCTUREOFTHEFISHGENUSDelminichthys
I.Buj1,Z.Mari1,M.aleta2,T.Mihinja1,R.anda3,D.Zanella1,P.Mustafi1,M.Mrakovi1
1
DepartmentofZoology,FacultyofScience,Zagreb,Croatia(ivaradic@biol.pmf.hr)
2
FacultyofTeacherEducation,Zagreb,Croatia
3
NationalMuseum,Prague,CzechRepublic
ThefreshwatersystemsoftheDinarickarstregionmaintainexceptionallyrichfishcommunity
with great portion of endemic species. As a possible explanation of such diversity several
events and phenomena have been proposed: geographic isolation of rivers and their
undergroundconnections,geologicalhistory,iceagerefuges,specificmutationrates.Contrary
tothemajorityofthefishgenera,genusDelminichthysinhabitsgeographicallyveryrestricted
area and comprises only four species. In order to reveal population genetic structure and
demographic history of each Delminichthys species as a contribution to explanation of
evolution in Dinaric water systems, this investigation was designed as a combination of
phylogeographic and population genetic analyses of all Delminichthys species. Based on
mitochondrial and nuclear DNA, we have investigated phylogenetic rela tionships, genetic
diversity and differentiation, as well as intraspecific genetic structure of four Delminichthys
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12.HBK4412 CBC
species.Themostimportantresultsincludepronouncedmitonucleardiscordance,moderate
tohighgeneticdiversityinsidethemajorityofpopulationsandsignificantgeneticstructuring.
Previously reported data on the underground gene flow will be discussed in the light of the
newlyobtainedresults.
Keywords:Dinarickarst,freshwatersystems,populationstructure,geneticdiversity,geneflow
O37
FILOGENIJA I FILOGEOGRAFIJA BJELONOGOG RAKA (Austropotamobius pallipes KOMPLEKS)
URIJEKAMAJADRANSKOGBAZENA
M.Jeli1,G.Klobuar1,F.Grandjean2,J.Amouret3,D.Franjevi1,M.Futo4,D.Ghia5,G.Fea5,M.
Govedi6,M.Vuci1,N.Puillandre7,I.Maguire1
1
Sveuilite u Zagrebu, Prirodoslovnomatematiki fakultet, Bioloki odsjek, Zoologijski zavod,
Zagreb,Croatia(misel.jelic@biol.pmf.hr)
2
UniversitydePoitiers,UMR7267EcologieetBiologiedesInteractions,Poitiers,Francuska
3
UniversityofIceland,DepartmentofLifeandEnvironmentalSciences,Reykjavk,Island
4
University of Mnster, Institute for Evolution and Biodiversity, Animal Evolutionary Ecology
Group,Mnster,Njemaka
5
University of Pavia, Dipartimento di Scienze della Terra e dellAmbiente, Laboratorio Acque
Interne,Pavia,Italija
6
CentreforCartographyofFaunaandFlora,Ljubljana,Slovenija
7
Musum National dHistoire Naturelle, Dpartement Systmatique et Evolution, UMR 7138
Systmatique, Adaptation et Evolution UPMCIRDMNHNCNRS (UR IRD 148), CP 26, Paris,
Francuska
Kljunerijei:mitohondrijskibiljezi,mikrosateliti,morfometrija,prekojadranskiputoviirenja
PHYLOGENYANDPHYLOGEOGRAPHYOFTHEWHITECLAWEDCRAYFISH(Austropotamobius
pallipesCOMPLEX)INTHEADRIATICBASINRIVERS
M.Jeli1,G.Klobuar1,F.Grandjean2,J.Amouret3,D.Franjevi1,M.Futo4,D.Ghia5,G.Fea5,M.
Govedi6,M.Vuci1,N.Puillandre7,I.Maguire1
1
UniversityofZagreb,FacultyofScience, Department ofBiology,DivisionofZoology,Zagreb,
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12.HBK4512 CBC
Croatia(misel.jelic@biol.pmf.hr)
2
UniversitydePoitiers,UMR7267EcologieetBiologiedesInteractions,Poitiers,France
3
UniversityofIceland,DepartmentofLifeandEnvironmentalSciences,Reykjavk,Island
4
University of Mnster, Institute for Evolution and Biodiversity, Animal Evolutionary Ecology
Group,Mnster,Germany
5
University of Pavia, Dipartimento di Scienze della Terra e dellAmbiente, Laboratorio Acque Interne,
Pavia,Italy
6
CentreforCartographyofFaunaandFlora,Ljubljana,Slovenia
7
Musum National dHistoire Naturelle, Dpartement Systmatique et Evolution, UMR 7138
Systmatique,AdaptationetEvolutionUPMCIRDMNHNCNRS(URIRD148),Paris,France
Systematicsofthewhiteclawedcrayfishiscontinuouslydebatedandassuchcausedproblems
for effective conservation and population management. To improve the knowledge on this
endangeredspeciescomplex,itsphylogeneticrelationshipsandspeciesphylogeographywithin
the Adriatic Basin were revised using results from analyses of mitochondrial DNA (mtDNA),
codominant nuclear DNA markers (microsatellites), and morphometrics. Analyses on mtDNA
resulted with description of new lineages. Distribution of mtDNA lineages has regular
geographicpatternandcanberelatedtogeologicalhistoryoftheAdriaticregiondatedfrom
MessiniansalinitycrisisuntilPleistocenemarineregressions.Microsatellitesanalysesrevealed
introgression and admixture of nuclear genome between distinct mtDNA lineages. Results of
morphometricalanalysesareinlinewiththegeneticstructureobtainedfrommicrosatellites
analyses. The obtained results enabled definition of Evolutionary Significant Units and
Management Units, useful in future conservation and management of endangered white
clawedcrayfishpopulationsintheAdriaticSeabasins.Also,itconfirmsanecessityofmultiple
analyses application (molecular and morphological) in managing endangered species
populations.
Keywords:mitochondrialmarkers,microsatellites,morphometrics,transAdriaticdispersalroutes
O38
PRVIREZULTATIINVENTARIZACIJEVRSTAOHARA,BOGOMOLJKIIRAVNOKRILACA(INSECTA:
Polyneoptera)IREGPODRUJAPLANINEDINARE
F.Rebrina1,J.Skejo1,N.Tvrtkovi2
1
SveuiliteuZagrebu,Prirodoslovnomatematikifakultet,UdrugastudenatabiologijeBIUS,
Zagreb,Hrvatska(rebrinafran@gmail.com)
2
NaturaDrutvozazatituprirodeHrvatske,Zagreb,Hrvatska
FaunakukacaizskupinePolyneopteranapodrujuplanineDinareuprolostijerazmjernoslabo
istraivana,stekvrlomalimbrojemobjavljenihpodataka.Tijekomistraivanjaprovedenog2012.i
2013. godine posjeeno je ukupno 19 podrobnije istraenih lokaliteta, a 68 vrsta ohara,
bogomoljki iravnokrilaca zabiljeeno je poprvi put za ovo podruje. Za jednu vrstu ovo jeprvi
nalaznaprostoruHrvatske,apronaenojeinekolikosvojtisjonerazjenjenimtaksonomskim
poloajem.Naisurezultatiobjedinjenisasvimdostupnimliteraturnimimuzejskimpodacimaza
ovo podruje, otkrivajui ukupno ak 98 svojti, s ciljem dobivanja to potpunije slike o fauni
kukacaizskupinePolyneopteranaprostoruplanineDinareinjezineokolice.
Kljunerijei:faunistika,redoviPolyneoptera,Hrvatska,Dinaridi
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F.Rebrina1,J.Skejo1,N.Tvrtkovi2
1
UniversityofZagreb,FacultyofScience,BiologyStudentsAssociationBIUS,Zagreb,Croatia
(rebrinafran@gmail.com)
2
NaturaSocietyforNatureProtectionofCroatia,Zagreb,Croatia
ThepolyneopteraninsectfaunaofDinaraMountainareawaspoorlystudiedinthepast,withonly
scarcedatapublishedonthetopic.Duringaresearch,conductedin2012and2013,atotalof19
betterinvestigatedlocalitieswerevisitedand68speciesofBlattodea,MantodeaandOrthoptera
wererecordedforthefirsttimeintheinvestigatedarea.Onespecieswasrecordedforthefirst
time for Croatia, while a couple of findings include taxa with presently unresolved taxonomic
status. Our results were combined with all available museum and literature data for this area,
revealingasmuchas98taxaaltogether,inordertocompletethepictureofpolyneopteraninsect
faunainhabitingDinaraMt.anditssurroundings.
Keywords:faunistics,Polyneopteranorders,Croatia,DinaricAlps
O39
MOLEKULARNOFILOGENETIKA I FILOGEOGRAFSKA ANALIZA POPULACIJA VRSTE
Holandrianaholandrii(C.PFEIFFER,1828)(MOLLUSCA:GASTROPODA)UHRVATSKOJ
L.Lovreni1,J.Lajtner2,M.Podnar3
1
MirkaBaklie6,SvetiKriZaretje,Hrvatska(leona.lovrencic@gmail.com)
2
Biolokiodsjek,Prirodoslovnomatematikifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
3
Hrvatskiprirodoslovnimuzej,Zagreb,Hrvatska
SlatkovodnavrstapuaHolandrianaholandrii(Pfeiffer,1828)irokojerasprostranjenanapodruju
jugoistone Europe. Spada u porodicu Melanopsidae koja je vrstama i brojnou dominirala
Panonskim jezerom tijekom kasnog miocena i pliocena. Cilj ovog istraivanja bio je ustanoviti
filogenetske i filogeografske odnose populacija ove vrste na temelju analize dva genska biljega,
mitohondrijskihgenazaCOIi16SrRNA.Filogenetskiodnosirekonstruiranisuupotrebomrazliitih
filogenetikihmetodazasvakugenskuregijuzasebno,kaoinakonkateniranimsetovimasekvenci.
Molekularnofilogenetikeanalizeustanovilesupostojanjetrigeografskiigenetskiodijeljenegrupe
idvijepodgrupe.Dobivenevrijednostigenetskihudaljenostiukazujunarelativnomladeevolucijske
linijekojesuserazdvojiletijekompleistocena.
Kljunerijei:Melanopsidae,slatkovodnipuevi,filogenija,filogeografija,COIi16SrRNA
L.Lovreni1,J.Lajtner2,M.Podnar3
1
MirkaBaklie6,SvetiKriZaretje,Croatia(leona.lovrencic@gmail.com)
2
DepartmentofBiology,FacultyofScience,UniversityofZagreb,Zagreb,Croatia
3
CroatianNaturalHistoryMuseum,Zagreb,Croatia
Holandrianaholandrii(Pfeiffer,1828)isafreshwatersnail,widespreadinsoutheasternEurope.It
belongstothefamilyMelanopsidaewhichdominatedtheLakePannonbythenumberofspecies
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and their abundance during the late Miocene and Pliocene. The aim of this research was to
determine phylogenetic and phylogeographic relationships of populations of this species based
on the analysis of two genetic markers, mitochondrial COI and 16S rRNA genes. Phylogenetic
relationships were reconstructed using different phylogenetic methods for each gene region
individually as well as for concatenated sets of sequences. Molecular phylogenetic analyses
established the existance of three geographically and genetically separated groups and two
subgroups. The obtained values of genetic distances indicate relatively young evolutionary
lineageswhichhavedivergedduringthePleistocene.
Keywords:Melanopsidae,freshwatersnails,phylogeny,phylogeography,COI,16SrRNA
O40
UTJECAJ OBALNOG ONEIENJA NA EPIGENETIKU I REPRODUKTIVNI FITNES VOLKA
Hexaplextrunculus
M.rut1,A.tambuk1,A.Erdelez2,D.Polovi1,M.Furdek3,M.Peharda2,G.Klobuar1
1
Sveuilite u Zagrebu, Prirodoslovnomatematiki fakultet, Zoologijski zavod, Zagreb,
Hrvatska(msrut@biol.pmf.hr)
2
InstitutzaOceanografijuiRibarstvo,Split,Hrvatska
3
InstitutRuerBokovi,Zavodzaistraivanjemoraiokolia,Zagreb,Hrvatska
Kljunerijei:evolucija,populacijskaepigenetika,fitnes,oneienje
IMPACTOFCOASTALPOLLUTIONONEPIGENETICSANDREPRODUCTIVEFITNESSOFMARINE
SNAILHexaplextrunculus
M.rut1,A.tambuk1,A.Erdelez2,D.Polovi1,M.Furdek3,M.Peharda2,G.Klobuar1
1
Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
(msrut@biol.pmf.hr)
2
InstituteofOceanographyandFisheries,Split,Croatia
3
DivisionforMarineandEnvironmentalResearch,RuerBokoviInstitute,Zagreb,Croatia
Hexaplextrunculusisamarinegastropodwhosereproductivefitnesscanbeseverelyaffected
by very low concentration of antifouling compound trybutiltin (TBT). TBT has strong
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12.HBK4812 CBC
xenoandrogenimpactinsnails,causingdevelopmentofimposex,e.g.superimpositionofmale
sexualcharacteristicinfemales,therebyaffectingthefitnessofentirepopulations.TBTisalso
known as a DNA demethylating agent. The aim of this study was to unravel the interactions
betweenTBTbioaccumulation,reproductivefitness,epigeneticandgeneticendpointsinmurex
snail H. trunculus. Four populations inhabiting polluted sites with intense marine traffic and
boat maintenance activity and three inhabiting sites with low anthropogenic impact were
sampled in the coastal eastern Adriatic. Higher incidence of imposex was observed in snails
inhabiting polluted than the reference sites. Analysis of methylation sensitive amplification
polymorphism(MSAP)revealedexistenceofepigeneticdifferentiationamongpopulationsand
lackofgeneticpopulationstructure.LevelofDNAmethylationsignificantlycorrelatedwiththe
imposexlevel,suggestingepigeneticbackgroundofimposexdevelopment.
Keywords:evolution,populationepigenetics,fitness,pollution
O41
BRZAEVOLUCIJADAGNJEPODUTJECAJEMONEIENJAEKSPERIMENTALNAGENOMIKA
A.tambuk1,S.Dennis2,D.Polovi1,M.rut1,V.SoriaCarrasco2,Z.Gompert3,V.Bakovi4,G.
Klobuar1,P.Nosil2
1
Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu, Zagreb, Hrvatska
(astambuk@biol.pmf.hr)
2
DepartmentofAnimalandPlantSciences,UniversityofSheffield,Sheffield,UK
3
DepartmentofBiology,UtahStateUniversity,Logan,UT,USA
4
InstituteofForestEntomology,ForestPathologyandForestProtection(IFFF)attheUniversity
ofNaturalresourcesandLifeSciences,Wien,Austria
Kljunerijei:adaptacija,fitnes,genomskaarhitektura,genomskookolineinterakcije
A.tambuk1,S.Dennis2,D.Polovi1,M.rut1,V.SoriaCarrasco2,Z.Gompert3,V.Bakovi4,G.
Klobuar1,P.Nosil2
1
DepartmentofBiology,FacultyofScience,UniversityofZagreb,Croatia(astambuk@biol.pmf.hr)
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DepartmentofAnimalandPlantSciences,UniversityofSheffield,Sheffield,UK
DepartmentofBiology,UtahStateUniversity,Logan,UT,USA
4
InstituteofForestEntomology,ForestPathologyandForestProtection(IFFF)attheUniversity
ofNaturalresourcesandLifeSciences,Wien,Austria
Anthropogenic disturbance can rapidly and substantially alter the environment and exposed
organisms are challenged to quickly adapt to these changes. We studied evolutionary
responses to pollution in marine environments using the Mediterranean mussel Mytilus
galloprovincialis. We sampled 15 natural populations along a pollution gradient and used a
subsetofthesetoperformtransplantexperimentsbetweencleanandpollutedenvironments
in both the wild and in lab mesocosms. The results of the experiments showed consistent
differential survival between treatments and source populationsdriven by an acclimatization
effectinthewildtransplantandbypopulationbyenvironmentinteractionsinthemesocosm
experiment.Forover1800musselsusedinthesestudieswecollectedgenomewideSNPdata
using a genotypebysequencing approach. These SNPs were analysed to test for genetic
structure in natural populations and the genomic architecture of survival (i.e., fitness) in the
experiments. By combining data obtained from the native populations and two types of
transplant experiments we identified interactions between environment, genotype, and
fitness, thus providing new insight into pollution driven evolution and the effect of adaptive
processesonthemusselgenome.
Keywords:adaptation,fitness,genomicarchitecture,genotypeenvironmentinteraction
O42
GENETIKAARHITEKTURAMORFOLOKIHOBILJEJADAGNJEMytilusgalloprovincialis
D.Polovi1,S.Dennis2,A.tambuk1,M.rut1,V.SoriaCarrasco2,Z.Gompert3,V.Bakovi4,G.
Klobuar1,P.Nosil2
1
SveuiliteuZagrebu,Prirodoslovnomatematikifakultet,Zoologijskizavod,Zagreb,Hrvatska
(dorotea.polovic@biol.pmf.hr)
2
DepartmentofAnimalandPlantSciences,UniversityofSheffield,Sheffield,UK
3
DepartmentofBiology,UtahStateUniversity,Logan,UT,USA
4
InstituteofForestEntomology,ForestPathologyandForestProtection(IFFF)attheUniversity
ofNaturalresourcesandLifeSciences,Wien,Austria
Kljunerijei:GWAS,fenotipskaobiljeja,fitnes,oneienje
3
th
12.HBK5012 CBC
D.Polovi1,S.Dennis2,A.tambuk1,M.rut1,V.SoriaCarrasco2,Z.Gompert3,V.Bakovi4,G.
Klobuar1,P.Nosil2
1
Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
(dorotea.polovic@biol.pmf.hr)
2
DepartmentofAnimalandPlantSciences,UniversityofSheffield,Sheffield,UK
3
DepartmentofBiology,UtahStateUniversity,Logan,UT,USA
4
InstituteofForestEntomology,ForestPathologyandForestProtection(IFFF)attheUniversity
ofNaturalresourcesandLifeSciences,Wien,Austria
The genetic architecture of phenotypic traits, including their heritability, numbers of loci
involved, and effect sizes, are not well understood in bivalves. Anthropogenic pollution may
createenvironmentaldifferencesthatcanaltermussel(Mytilusgalloprovincialis)morphology
througheithergenotypicorinducedenvironmentaleffects.Toassessthegeneticarchitecture
of morphology we measured 15 morphometric traits in 1700 mussels from two populations
inhabitingsitesdifferinginpollutionpressure.Weusedagenotypebysequencingapproachto
generategenomicdatathatallowedustothendescribethegeneticarchitectureofthesetraits
inagenomewideassociationstudy.Additionally,weestimatedthegeneticarchitectureofthe
sametraitsaswellasproxiesforfitnessinanadditionalsampleof15populationsdistributed
across a pollution gradient using 20 mussels per population. The results thus characterise
variation within and among populations in mussel morphology and assess the association
betweengenotype,phenotypeandenvironment.
Keywords:GWAS,phenotypictraits,fitness,pollution
O43
ANALIZARAZNOLIKOSTIKLOROPLASTNEDNAIFILOGEOGRAFIJALJEKOVITEKADULJE(Salvia
officinalisL.)
N.Jantol,T.Keki
Botaniki zavod, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu, Zagreb, Hrvatska
(tadijakekic@gmail.com)
Kljunerijei:ljekovitakadulja,filogeografija,kloroplastnaDNA
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12.HBK5112 CBC
N.Jantol,T.Keki
Division of Botany, Faculty of Science, University of Zagreb, Zagreb, Croatia
(tadijakekic@gmail.com)
Understanding the genetic diversity and the phylogeography of Salvia officinalis L is the
prerequisiteforidentificationoftheoriginofthisspecies,aswellasforreconstructingitsareal
through the past. We analysed the sequence diversity of two chloroplast DNA regions of 86
individuals from 21 populations along the Adriatic coast and islands. After aligning the
sequences, a parsimony haplotype network was made using the TCS computer program.
Overall we found 9 chloroplast haplotypes. Four haplotypes were specific for southern
populations. Since the southern populations in addition to specific also have all the other
haplotypespresentinnorthAdriatic,weconcludedthattheyrepresentthegeneticsourcefor
thepropagationofthisspeciesasitspreadgraduallyafterthedeglaciationinthenorthwestern
direction, which left the north populations today genetically poorer because of the founder
effect. Populations from the south Adriatic, because of their genetic richness, re present
particularlyvaluablematerialforfuturebreedingprograms.
Keywords:commonsage,phylogeography,chloroplastDNA
O44
GENETSKA RAZNOLIKOST PRIRODNIH I KULTIVIRANIH POPULACIJA DALMATINSKE KADULJE
(SalviaofficinalisL.,LAMIACEAE)
12.HBK5212 CBC
Kljunerijei:Salvia,genetskaraznolikost,mikrosateliti
Salvia officinalis L., Dalmatian sage, is an outcrossing, subshrubby medicinal and aromatic
perennial.ThenaturaldistributionareaofS.officinalisiscoastalregionofthewesternBalkan
andsouthernApenninePeninsulasbutduetoitsmedicinal,culinaryandornamentalproperties
ithasbeenwidelycultivatedsinceancienttimes.Theaimofthisstudywastoinvestigatethe
geneticdiversityandpopulationstructureofthewild,cultivatedandnaturalizedpopulationsof
S.officinalisacrosstheBalkanPeninsula.Weusedeightmicrosatellitemarkerstogenotype709
samplesfrom30populations.Asexpected,nonnativepopulationsexhibitedsignificantlylower
allelic richness as well as gene diversity in comparison to native populations. The neighbour
joining tree showed that the most of the populations grouped together in accordance to
geographicalpositionoftheircollectingsites,fromSloveniaintheNorthWestoftheregionto
Greece in the SouthEast, with the exception of seven nonnative populations that grouped
separately from the rest, suggesting the common origin of the cultivated plant material. A
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Bayesiananalysisofthepopulationstructureidentifiedtwodominantgeneticclustersclearly
separating indigenous and cultivated/naturalized populations. The indigenous populations
furtherformedthreesubclustersindicatinggeographicalstructuring.
Keywords:Salvia,geneticdiversity,microsatellites
O45
KLONALNA STRUKTURA ENDEMINE I UGROENE KRATKOZUPASTE KADULJE Salvia
brachyodon (LAMIACEAE) UTVRENA MIKROSATELITNIM BILJEZIMA: ZNAENJE ZA
UPRAVLJANJEIZATITU
I.Radosavljevi1,Z.atovi2,Z.Liber1
1
Botaniki zavod, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska(ivanrad@biol.pmf.hr)
2
Zavodzasjemenarstvo,Agronomskifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
Salvia brachyodon Vandas ili kratkozupasta kadulja je klonalna, usko endemina i ugroena
biljnavrstastekdvijepoznatepopulacije:napoluotokuPeljecu(Hrvatska),tenaplaniniOrjen
(Bosna i Hercegovina). S ciljem utvrivanja razine genetike raznolikosti, na 120 jedinki iz
populacijesPeljecajeprovedenagenetikaanalizaupotrebomosammikrosatelitnihbiljega.S
namjerom utvrivanja detaljne prostornogenetike strukture, svakoj jedinci su pridruene
pripadajue geografske koordinate. Dobiveni rezultati potvruju klonalno ustrojstvo, te je
utvrenaprisutnostvelikihigustihsklopovagenetikiidentinihjedinkitoupuujenairenje
ove vrste stolonima. Za tako male i izolirane populacije, ovakva strategija razmnoavanja
moda i predstavlja kratkoronu prednost, ali imajui na umu da se kao mogue posljedice
klonalnosti mogu po javiti izraeno krianje u bliskom srodstvu te smanjena reproduktivnost,
takoerpredstavljaivelikuprijetnju.Nadalje,utvrenajeiprisutnostpopulacijskoguskoggrla
ime je dodatno naglaena ugroenost ove populacije. Ekoloka sukcesija i nestanak
odgovarajueg stanita otvorenog tipa su vjerojatni razlozi tako izraenog smanjenja ove
populacija,dokjujepoar,kojijeprije12godinaopustoioovajlokalitet,vjerojatnospasiood
daljnjegpropadanja.Sciljemnjezinezatite,agropastoralnibisesustavkojijeuprolostina
tompodrujubioprisutan,trebaopoticatiiponovnouspostaviti.
Kljunerijei:Salviabrachyodon,klonalnost,zatita,mikrosateliti
I.Radosavljevi1,Z.atovi2,Z.Liber1
1
DepartmentofBotany,DivisionofBiology,FacultyofScience,UniversityofZagreb,Zagreb,
Croatia(ivanrad@biol.pmf.hr)
2
Department of Seed Science and Technology, Faculty of Agriculture, University of Zagreb,
Zagreb,Croatia
SalviabrachyodonVandasorshorttoothsageisaclonal,narrowendemicandendangered
plant species with only two known populations: on Peljeac peninsula (Croatia) and Mt.
Orjen (Bosnia and Herzegovina). In order to assess the levels of its genetic diversity, 120
individuals from Peljeac peninsula population were genetically analysed using eight
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12.HBK5412 CBC
microsatellitemarkers.Withintentionofreachingfinescaleintrapopulationspatialgenetic
structure, geographic coordinates for each plant sample were recorded. Given results
confirmedthehighlyclonalstructureofthisspecies.Spatialgeneticanalysisrevealedlarge
and tightly packed stands of ramets suggesting the propagation by underground stolones.
Forsmallandisolatedpopulation,suchreproductivestrategyismaybeanadvantageinthe
shortterm scale, but bearing in mind that clonality can have development of inbred
populationwithlowfecundityforaconsequence,italsopresentsagreatthreat.Inaddition,
population bottleneck was also detected, confirming the high vulnerability of this
population. Ecological succession and disappearance of suitable opentype habitat are
recognized as the likely causes of such dramatic reduction in population size. In contrast,
large scale wild fire that has overrun this location 12 years ago is presumably population
saviour. In order to protect this population, agropastoral system that was once present in
thislocation,shouldbeencouragedandreestablished.
Keywords:Salviabrachyodon,clonality,conservation,microsatellites
O46
EVOLUCIJA,RAZNOLIKOSTIFILOGENIJA5SrDNAJEDINICAPONAVLJANJAUDIPLOIDNIMI
POLIPLOIDNIMVRSTAMAIZTRIBUSAAnemoninae(Ranunculaceae)
J.Mlinarec1,D.Franjevi2,L.Bokor3,V.Besendorfer1
1
Zavod za molekularnu biologiju, Bioloki odsjek, Prirodoslovnomatematiki fakultet,
SveuiliteuZagrebu,Zagreb,Hrvatska(vbesend@biol.pmf.hr)
2
Zoologijski zavod, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u
Zagrebu,Zagreb,Hrvatska
3
InternationalCenterforGeneticEngineeringandBiotechnology(ICGEB),Trst,Italija
Tribus Anemoninae broji oko 200 vrsta koje su na temelju osnovnog broja kromosoma
podijeljene u dva podroda: Anemone x=8 i Anemonidium x=7. U ovom radu istraena je
evolucijia, raznolikost i filogenija 5S rDNA u diploidima i poliploidima. Dobiveni rezultati
pokazali su da je razdvajanje vrsta u dva podroda praeno promjenama u 5S rDNA koje su
dovele do razlika u duini jedinica ponavljanja, sadraja GC baza i strukturi sekvenca.
Prisutnost 5S rDNA jedinica ponavljanja kraih od tipinih za vrste iz roda Anemonei duih
odtipinihzavrsteizrodaHepaticaupuujudaje5SrDNAprolakrozdvijerundeevolucije:
skraivanjeiproduljivanje.PrisutnostMITEliketranspozonskihelemenatauvrstamaizroda
Hepatica ukazuje na transpozonima posredovanu amplifikaciju 5S rDNA. Razmjerno visoka
razina unutarvrsne raznolikosti, prisutnost varijanti razliite du ine i pojava pseudogena
ukazuje na djelovanje slabih homogenizacijskih sila na oblikovanje 5S rDNA. Filogenetska
istraivanjabaziranana5SrDNApodupiruhipotezudajeA.parvifloradonorDsubgenomau
alopoliploidnimvrstamaA.multifidaiA.baldensis.Procesdiploidizacijeuobaalopoliploida
odvijao se u smjeru strukturnih promjena 5S rDNA kao to je gubitak 5S rDNA iz D
subgenomaizamjenas5SrDNAizBsubgenoma.FilogenijavrstaizrodaHepaticapotvrdila
jealopoliploidnoporijeklovrstaH.transsilvanicaiH.yamatutaiukazujuinavrsteH.nobilisi
H.insulariskaopotencijalneroditeljeovihalopoliploida.
Kljunerijei:5SrDNA,Anemone,Pulsatilla,Hepatica,alopoliploidi,usklaenaevolucija
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12.HBK5512 CBC
J.Mlinarec1,D.Franjevi2,L.Bokor3,V.Besendorfer1
1
DivisionofMolecularBiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(vbesend@biol.pmf.hr)
2
DivisionofZoology,Departmentof Biology, FacultyofScience,UniversityofZagreb,Zagreb,
Croatia
3
InternationalCenterforGeneticEngineeringandBiotechnology(ICGEB),Trieste,Italy
The tribe Anemoninae is composed of ~200 species divided into two subgenera according to
the basic chromosome number: Anemone x=8 and Anemonidium x=7. We have performed
extensive study of evolution, diversity and phylogeny of 5S rDNA in diploid and polyploid
speciesofthetribeAnemoninae.OurresultsshowthatdiversificationofAnemoninaeintotwo
subgenerawasaccompaniedwithmajorchangesin5SrDNAthatledtodifferent5Sunitarray
length,GCcontentand5SrDNAsequencestructure.Existenceof5Sshorterthantypicalunit
size variants in Anemone and longer than typical in Hepatica indicates that 5S rDNA has
undergone two rounds of evolution: contraction and extension. The presence of MITElike
transposable elements in Hepatica suggests that amplification of 5S rDNA is transposon
mediated. 5S rDNA of the Anemoninae tribe was shaped byweek homogenization fo rces as
indicatedbyhighintraindividualnucleotidediversity,presenceof5Sunitarraylengthvariants
andpseudogenes.PhylogeneticstudysupportsthehypothesisthatA.parvifloracouldbethe
donoroftheDsubgenometoallopolyploidsA.multifidaandA.baldensis.Bothallopolyploids
undergone diploidization process including loss of 5S rDNA from the D subgenome and
replacement with those of the B subgenome. Phylogeny of Hepatica confirmed allopolyploid
originofH.transsilvanicaandH.yamatutaiandsuggeststhatH.nobilisandH.insulariscould
beconsideredastheirputativeparentalspecies.
Keywords:5SrDNA,Anemone,Pulsatilla,Hepatica,allopolyploidy,concertedevolution
O47
EVOLUCIJAMAHOVNJAKAREKOLONIZACIJOMSLATKOVODNIHEKOSUSTAVA
N.Koleti1,2,M.Novosel2,N.Kevi2,D.Franjevi2
1
Institut za istraivanje i razvoj odrivih ekosustava, Velika Gorica, Hrvatska
(nickoletic@gmail.com)
2
Biolokiodsjek,Prirodoslovnomatematikifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
Mahovnjaci su vodeni beskraljenjaci koji nespolnim pupanjem ine velike kolonije. Svaka
jedinka unutar kolonije funkcionira kao zasebni zooid te je genetiki identina svakoj drugoj
jedinci unutar iste kolonije. Veina slatkovodnih vrsta mahovnjaka pripada razredu
Phylactolaemata, dok par vrsta koje podnose boatu vodu pripadaju razredu Gymnolaemata.
Mahovnjacisuuzorkovaniurijekamajadranskogidunavskogslivateumovarnimpodrujima
kontinentalnogdijelaHrvatske.Slatkovodneiboatevrstemahovnjakagenetikisuanalizirane
s ciljem utvrivanja filogenetskih odnosa izmeu slatkovodnih i boatih vrsta unutar razreda
PhylactolaemataiGymnolaematateodreivanjaulogeboatihvrstaukolonizacijislatkovodnih
imorskihekosustava.Filogenetskiodnosidobivenianalizomgenaza18SrRNA,28SrRNA,COIi
regije ITS2 potvrdili su Phylactolaemata kao bazalnu grupu unutar skupine mahovnjaka.
Komparativna analiza postojeih saznanja o filogeniji mahovnjaka proirena evolucijskom
hipotezom predlae model naseljavanja morskih i slatkovodnih ekosustava od strane skupine
mahovnjaka tijekom njihove evolucijske prolosti. Boate svojte mahovnjaka predstavljaju
poveznicu za ovu ekolokofilogenetsku hipotezu. Usporedba boatih vrsta mahovnjaka
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12.HBK5612 CBC
Kljunerijei:COI,Gymnolaemata,ITS2,Phylactolaemata,rRNAgeni
EVOLUTIONOFBRYOZOANSVIARECOLONISATIONOFFRESHWATERECOSYSTEMS
N.Koleti1,2,M.Novosel2,N.Kevi2,D.Franjevi2
1
Institute for Research and Development of Sustainable Ecosystems, Velika Gorica, Croatia
(nickoletic@gmail.com)
2
DepartmentofBiology,FacultyofScience,UniversityofZagreb,Zagreb,Croatia
Bryozoans are aquatic invertebrates that form large colonies by asexual budding. Each
individualwithinacolonyworksasaseparatezooidandisgeneticallyidenticaltoeachother
individual within the same colony. Most freshwater species of bryozoans belong to the
Phylactolaemata class, while several species that tolerate brackish water belong to the
Gymnolaemata class. Bryozoan samples were collected in the rivers of Adriatic and Danube
basin and in the wetland areas in the continental part of Croatia. Freshwater and brackish
taxons of bryozoans were genetically analyzed for the purpose of creating phylogenetic
relationships between freshwater and brackish taxons of the Phylactolaemata and
Gymnolaemata classes and determining the role of brackish species in colonizing freshwater
and marine ecosystems. Phylogenetic relationships inferred on the genes for 18S rRNA, 28S
rRNA, COI and ITS2 region confirmed Phylactolaemata bryozoans as radix bryozoan group.
Comparative analysis of existing knowledge about the phylogeny of bryozoans and the
expansion of known evolutionary hypotheses is proposed with the model of settlement of
marine and freshwater ecosystems by the bryozoans group during their evolutionary past.
Brackishbryozoantaxonsrepresentalinkforecologicalphylogenetichypothesis.Comparison
of brackish bryozoan species Lophopus crystallinus and Conopeum seurati confirmed a dual
colonizationoffreshwaterecosystemsthroughouttheevolutionofthisgroupofanimals.
Keywords:COI,Gymnolaemata,ITS2,Phylactolaemata,rRNAgenes
O48
ROOMTOMANEUVER:PHYSICALENVIRONMENTCONSTRAINTSTHEPATTERNANDRATEOF
CARAPACESHAPEEVOLUTIONINCHELONIANS
M.Djurakic1,A.Herrel2
1
Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad,
Serbia(marko.djurakic@dbe.uns.ac.rs)
2
UMR7179CNRS/MNHNDpartmentd'EcologieetdeGestiondelaBiodiversit,ParisCedex5,
France
The physical environment plays an important role in driving the rate and pattern of
morphological evolution by constraining developmental pathways in particular directions at
ontogenetic and historical time scales. Therefore, understanding morphological evolution
requires consideration of developmental, biomechanical and phylogenetic approaches. Here
weinvestigatedtherateandpatternofcarapaceshapeevolution(phylogeneticapproach)in
relationtoaquaticandterrestrialmedia(biomechanicalapproach)intwoCheloniansuborders
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12.HBK5712 CBC
(PleurodiraandCryptodyra).Shellshapewasquantifiedby3Dgeometricmorphometrics,and
ratesofshapeevolutionwerecomparedusingatimecalibratedmolecularphylogeny.Patterns
ofshapeevolutionwereexaminedbycomparingevolutionaryratesforupper(vertebraland
costal plates) and lowercarapace (marginal plates) relative to the physical environment and
taxonomic affinity. We show that the rate of carapace evolution is greater in Cryptodira
compared to Pleurodira. The first phylogenetic principal component showed a gradient from
terrestrial to aquatic turtles highlighting the correlated evolution of morphology with the
physical environment. Terrestrial turtles exhibited greater rates for both carapace parts
comparedtoaquaticturtles.However,aquaticturtlesexhibitedagreaterevolutionaryratefor
the upper carapace compared to lower part, while the opposite pattern was observed for
terrestrialturtles.
Keywords:morphologicalevolutionaryrate,carapace,physicalenvironment,Chelonians
O49
A RECOGNITION OF SPECIES TAXON IS A TAIL OF MICROEVOLUTIONARY PROCESSES IN
Testudo marginata: AN EVIDENCE OF A SEXSPECIFIC ENVIRONMENTALLYINDUCED
VARIATIONOFPLASTRONMORPHOLOGY
M.Djurakic1,V.Milankov2
1
Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad,
Serbia(marko.djurakic@dbe.uns.ac.rs)
2
Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad,
Serbia
TheMarginatedtortoiseisthelargestEuropeantortoisewithchallengingtaxonomicstatus.For
instance, dwarf individuals from Messinia region in Peloponnese have been previously
proposedasaseparatespecies(Testudoweissingeri).Studiesthatutilizedmolecularmarkers
partlyconfirmedvalidityofT.weissingeri,butdifferentlyexplainedevolutionarysignificanceof
dwarfisminthistaxon.Here,weconductedamultilevelstudycombiningthreemitochondrial
genes (12s rRNA, cyt b, COI), comprehensivelyquantify plastron morphology and
environmentaldata(temperatureandprecipitation)inordertoassess:1)taxonomicvalidityof
T.weissingeri,2)influenceofenvironmentalpredictorsondwarfism.Plastronmorphology(size
andshape)ofadulttortoiseswasquantifiedbylinearand2Dgeometricmorphometrics.From
taxonomic perspective, dwarf form cannot be separated from nominal one, although male
specificsizeandsizerelatedshapedivergencewasobserved.Moreover,fivemalesoutof36
individuals defined as dwarf form showed diagnostic pattern of mitochondrial divergence.
Plastron size of sexes was influenced by different and independent environmental sources.
Likewise, in contrast to females, association between plastron shape and environmental
predictors was observed in males. Therefore, dwarfism is a consequence of sexspecific and
environmentalspecific factors. These findings are discussed from taxonomic,
microevolutionaryanddevelopmentalperspective.
Keywords:multilevelstudy,mitochondrialgenes,plastron,sexspecificfactors,environmental
specificfactors
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12.HBK5812 CBC
IO1
KOMPARATIVNA FILOGEOGRAFIJA TRI VRSTE ZAPADNOBALKANSKIH GUTERICA (REPTILIA,
LACERTIDAE)PREKLAPAJUIHAREALA
M.Podnar1,I.Grbac1,N.Tvrtkovi1,B.BruvoMaari2,W.Mayer3
1
Hrvatskiprirodoslovnimuzej,Zagreb,Hrvatska(martina.podnar@hpm.hr)
2
Zavodzamolekularnubiologiju,InstitutRuerBokovi,Zagreb,Hrvatska
3
CentralResearchLaboratories,NaturalHistoryMuseumVienna,Be,Austrija
M.Podnar1,I.Grbac1,N.Tvrtkovi1,B.BruvoMaari2,W.Mayer3
1
CroatianNaturalHistoryMuseum,Zagreb,Croatia(martina.podnar@hpm.hr)
2
Molecularbiologydivision,RuerBokoviInstitute,Zagreb,Croatia
3
CentralResearchLaboratories,NaturalHistoryMuseumVienna,Vienna,Austria
TherockclimbinglizardsDalmatolacertaoxycephalaandDinarolacertamosorensisandthe
grounddwelling lizard Podarcis melisellensis are Western Balkans endemics having largely
overlapping distribution range. Comparative phylogeographic study of those species based
on two mitochondrial genes revealed highly nonconcordant phylogeographic patterns
regardingthenumber,ageandgeographicdistributionoflineages.Theexistenceofseveral
possible microrefugia is implicated for all three species. While P. melisellensis and D.
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12.HBK5912 CBC
oxycephalasurvivedtheunfavourablePleistoceneclimaticconditionsinglacialrefugia,forD.
mosorensis, interglacial microrefugia appear more likely. Observed differences can be
explained in the light of different responses to Pleistocene climatic oscillations due to
specificecologicalandhabitatpreferencesaswellastheimpactofpastgeotectonicevents
but also the anthropogenic translocations. Within D. oxycephala, presently monotypic
species,twodeeplyseparatedcladesthatdivergedsome5myagowerefound.Thegenetic
distancesbetweenthemarecomparableorevenexceedthosefoundbetweensomeclosely
related Lacertid lizards. Observed cryptic genetic diversity within D. oxycephala prompts a
revision of its taxonomic and conservation status. Preliminary analyses of two nuclear
molecular markers confirmed the existence of two clades within D. oxycephala, but also
indicatedtheisolatedhybridizationeventsinthesecondarycontactzone.
IO2
ZARAZNEBOLESTIVODOZEMACATOSEVEZNA,TOJENOVO,ATOTREBAIMATINA
UMUKAKOBISESPRIJEILONJIHOVOIZUMIRANJE
M.Luka
Zavodzabolestiperadisklinikom,Veterinarskifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
(maja.lukac@vef.hr)
Vodozemci su danas jedna od najugroenijih ivotinjskih vrsta na svijetu. Osim gubitka stanita,
promjene klime i zagaenja, jedan od bitnih uzroka izumiranja vodozemaca su i zarazne bolesti.
Kakoljudiimajuvelikuuloguuirenjuiprenoenjuzaraznihbolestisjednogmjestanadrugo,vrloje
vano znati koje bolesti uzrokuju ugibanja vodozemaca, kako ih dijagnosticirati, te kako sprijeiti
njihovoirenje.Upredavanjueseiznijetidosadanjasaznanjaogljivinimivirusnimuzronicima
bolesti vodozemaca, ponajprije o hitrid gljivama Batrachochytrium dendrobatidis (BD), poznatom
uzronikumasovnihugibanjavodozemacaucijelomsvijetu,ionedavnoopisanomBatrachocytrium
salamandrivorans(BS)koji,premadosadanjimsaznanjima,uzrokujebolestiuginuasamorepaa
(urodela),teoranavirusu,kojimoeizazvatioboljevanjavodozemaca,gmazovairiba.Iznijetesei
rezultatipreliminarnihistraivanjainfekcijaBDom,BSomiranavirusomuovjejeribice(Proteus
anguinus)provedenihusklopuprojektazatiteovjejeribiceuHrvatskoj,kaoirezultatitestiranja
ostalih vodozemaca s razliitih lokacija u Hrvatskoj. Svrha predavanja je strunjake koji se bave
vodozemcima to detaljnije upoznati sa zaraznim i pogubnim bolestima vodozemaca, kako bi u
svomsvakodnevnomradu,osobitonaterenu,znaliprepoznatibolest,prikupitipotrebneuzorkei
sprijeitiirenjebolestisjednogpodrujanadrugo.
EMERGINGAMPHIBIANDISEASESWHATISKNOWN,WHATISNEWANDWHATSHOULDBE
BORNEINMINDTOPREVENTTHEIREXTINCTION
M.Luka
Department of Poultry Diseases with Clinic, Faculty of Veterinary Medicine, University of
Zagreb,Zagreb,Croatia(maja.lukac@vef.hr)
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12.HBK6012 CBC
Amphibians currently belong to a group of most endangered animal species in the world. In
additiontothelossofhabitats,climatechangesandpollution,contagiousdiseasesarealsoone
ofsignificantcausesofamphibianextinction.Sincetheroleofhumansindiseasespreadingand
transfer from one area to another is significant, it is very important to learn more about
emerging diseases in amphibians, how to diagnose those diseases and how to prevent their
spread.In this lecture current knowledge regarding the following fungal and viral causes of
emerging amphibian diseases will be presented, specifically about the: (i) chytrid fungus
Batrachochytrium dendrobatidis (BD), a well known cause of mass amphibian mortality
worldwide;(ii)anotherrecentlydescribedchytridfungusBatrachochytriumsalamandrivorans
(BS) which is, to our current knowledge, the cause of disease and mortality in Caudata
(Urodela)only;and(iii)Ranaviruswhichcancausediseaseinamphibians,reptilesandfishes.
TheresultsofpreliminaryinvestigationofBD,BSandRanavirusinfectionsintheolms(Proteus
anguinus),andofotheramphibiansfromCroatiawillalsobepresented.Theaimofthislecture
is to provide the herpetologists with more information about infectious and deleterious
amphibian diseases, to help them in their everyday activities, particularly in the field, to
recognize disease, collect adequate samples and prevent disease spread from one site to
another.
Keywords:Amphibians,Emergingdiseases,Batrachochytriumdendrobatidis,Batrachochytrium
salamandrivorans,Ranavirus
O50
DISTRIBUTION, ABUNDANCE AND DETECTION PROBABILITY OF LACERTIDAE IN FOUR
CROATIANISLANDS
E.PadoaSchioppa,M.Polo
UniversityofMilanoBicocca,DepartmentofEarthandEnvironmentalSciences,Milano,Italy
(emilio.padoaschioppa@unimib.it)
The evaluation of real abundance of species like reptiles may be some time problematic
because those species may be very sensitive to environmental and weather conditions.
Consequently the possibility to evaluate the detection probability may be an important
result(McDiarmidetal.,2012).Furthermore,inMediterraneanislands,thedistributionsof
reptilesisstillincomplete(Ficetolaetal.,2014).Duringthespringof2014wecarriedoutan
extensive survey in four island of Croatia: Losinj, Unije, Susak, Ilovik. The aims of this field
campaign were:1. to assess the abundance of more common species of Lacertidae; 2. to
evaluatetheusefulnessofdetectionprobabilitymeasuredbymeansofrepeatedtransects;
3. to describe the microhabitat elements that may have an important role in species
abundance.We covered the islands with 60 transect (36 Losinj, 10 Unije, 6 Susak, 8 Ilovik),
eachtransectwasrepeatedtwoorthreetimes.BymeansofRpackageandofAICapproach
we built models for three species (Algyroides nigropuntaus, Podarcis melliselensis and
Podarcis siculus) we were able to get models of abundance. The most abundant is P.m.
clearlylinkedtomicrohabitatsuchasstonewalls.Therepetitionoftransectsmayincreasea
lottheavailableinformation,altoughifinlargeareasmayrequireasignificativeamountof
time.Butafterarepetitionthepossibilitytocasualdetectiondecreasealotandthegeneral
resultisreliable.
Keywords:Detectionprobability,Herpetofauna,Islandbiogeography,Speciesabundance
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O51
UESTALOST IVORODNE GUTERICE, Zootoca vivipara Lichtenstein 1823 NA PODRUJU
ZAPADNOGBALKANA
S.Bakiera,D.Jeli
Hrvatskiinstitutzabiolokuraznolikost,HrvatskoherpetolokodrutvoHYLA,Zagreb,Hrvatska
(senka.bx@gmail.com)
ivorodnaguterica,ZootocaviviparaLichtenstein1823,jemaliguterizporodiceLacertidaepoznat
potometoimadvanainarazmnoavanja.ivorodnagutericaimanajveepodrujegeografske
rasprostranjenosti od svih gutera. Ona naseljava vei dio Europe i sjeverni dio Azije. Njen areal
ukljuuje i Hrvatsku te druge zemlje zapadnog Balkana. Geografska rasprostranjenost ivorodne
guterice na zapadnom Balkanu predstavljena u ovom radu odreena je na temelju objavljenih
podataka uz dodatak neobjavljenih terenskih podataka sakupljenih za Hrvatsku i Bosnu i
Hercegovinu. Koritene su etiri WorldClim varijable kako bi se opisali ekoloki parametri unutar
ovogpodruja.Oekivalismodaedobivenipodaciupuivatinapovezanosthrvatskihisusjednih
populacija (kako je u prethodnim publikacijama dokazana filogenetska povezanost hrvatske
populacije sa slovenskom i talijanskom populacijom). Znaajne razlike su pronaene u ekolokim
parametrima koje odabiru pojedine podpopulacije. ivorodna guterica moe ivjeti na razliitom
tipustanita,odnizinadovisokihplanina.Rezultatipokazujudapostojetriodvojenepodpopulacije
u prouavanom podruju i one odgovaraju poznatim podvrstama ivorodne guterice Zootoca
vivipara vivipara Von Jacquin, 1787, Zootoca vivipara carniolica Mayer, Bhme, Tiedemann i
Bischoff,2000iZootocaviviparapannonicaLciKluch,1968.
Kljunerijei:rasprostranjenost,Zootocavivipara,Hrvatska,ZapadniBalkan
HOW COMMON IS THE COMMON LIZARD, Zootoca vivipara Lichtenstein 1823 IN WEST
BALKANAREA?
S.Bakiera,D.Jeli
Croatian Institute for Biodiversity, Croatian Herpetological Society HYLA, Zagreb, Croatia
(senka.bx@gmail.com)
The common lizard, Zootoca vivipara Lichtenstein 1823, also called the viviparous lizard, is a
smalllacertidlizardwellknownforitsreproductivebimodality.Ithasthelargestgeographical
distribution ofall lizards. It inhabitsalmostall ofEurope andnorthernpart ofAsia. Itsrange
also includes Croatiaand other Western Balkancountries. The geographicdistributionof the
common lizard in the Western Balkan presented in this talk was determined according to
published data with addition of unpublished field data from Croatia and Bosnia and
Herzegovina. We used four basic WorldClim variables to describe the preferred ecological
parameters within this range. We expected that these data would prove connectivity of
Croatian populations with populations of neighbouring countries (as its phylogenetic
relationshipwasshownbetweenCroatianpopulationandSlovenianandItalianpopulationsin
previous publications). Significant differences were found in ecological parameter preference
between all three subpopulations. The common lizard can inhabit various types of habitats,
from lowlands to high mountains. Results show that three separate subpopulations,
corresponding to three subspecies of common lizard, Zootoca vivipara vivipara Von Jacquin,
1787, Zootoca vivipara carniolica Mayer, Bhme, Tiedemann i Bischoff, 2000 and Zootoca
viviparapannonicaLciKluch,1968,arepresentintheresearchedarea.
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Keywords:distribution,Zootocavivipara,Croatia,WesternBalkan
O52
DISTRIBUTION (HORIZONTAL AND VERTICAL) AND CONSERVATION STATUS OF
HERPETOFAUNAOFSARAJEVOCANTONANDITSWIDERAREA(BOSNIAANDHERZEGOVINA)
A.Zimi
HerpetologicalAssociationinBosniaandHerzegovinaAtra,Sarajevo,BosniaandHerzegovina
(bhhu.atra@gmail.com)
Thispaperpresentsdataaboutthefindingsofamphibianandreptilespeciesfromthewiderarea
of Sarajevo Canton in Bosnia and Herzegovina (B&H). As the capital of B&H, Sarajevo is
proclaimed as the most polluted city in the country and sometimes even Europe. Collection of
datawasmostlymadethroughfieldinvestigationsandadditionallybyanalyzesofliteraturedata
soastheprivateherpetologicalcollection"Lelo"andcollectionoftheNationalHistoryMuseum
ofB&H.Datashowthattheareaisinhabitedby13amphibianand18reptilespecies(61%ofB&H
herpetofauna). However, there are few suspicious and unspecific findings. Some species (Rana
temporaria, Podarcis melisellensis, Dalmatolacerta oxycephala) encounter their distribution
bordersinthisarea.Itisassumedthatthemajorityofrecordedpopulationsofherpetofaunahave
anintensivedecreasingtrendwithsomepopulationsalreadyextincted(eg.Bombinavariegata,
Vipera berus) due to strong anthropogenic impact, habitat changing and niche shifting. In
addition,onepopulationofTrachemysscriptawasfoundwhichrepresentsthefirstdataofthis
invasivealochtonespeciesforB&H.Paperalsoprovidesnewdataonerythronotusmutationin
Lacertaagilisbosnica.Conservationstatus(localandregional)aswellassuggestedconservation
measurethatcanbeintroducedtoimprovethecurrentsituationarealsodiscussed.
Keywords:Biodiversity,Amphibia,Reptilia,threats,protection
O53
REZULTATI PRVE GODINE SUSTAVNIH ISTRAIVANJA SASTAVA, DISTRIBUCIJE I BROJNOSTI
HERPETOFAUNENP"PLITVIKAJEZERA"
E.Kleteki,S.Leiner,M.Podnar
Hrvatskiprirodoslovnimuzej,Zagreb,Hrvatska(Eduard.Kletecki@hpm.hr)
Nacionalni park "Plitvika jezera" pripada najstarijim zatienim dijelovima prirode u Republici
Hrvatskoj,nounatotomuistraivanjabrojnihivotinjskihskupinakojeganastanjujunalazesena
samimpoetcima.Takvojestanjeisistraivanjemvodozemacaigmazova.Sporadinihbiljekio
herpetofauniovogapodrujaimamojoizvremenaaustrougarskogimperija,alinjenapotpuna
inventarizacija i sustavno istraivanje nije nikada provedeno. Sa stajalita herpetofaune, NP
"Plitvika jezera" smjeten je na zoogeografski izuzetno zanimljivom prostoru graninom
podruju rasprostranjenosti brojnih vrsta vodozemaca i gmazova. Osim toga u sastav parka,
pogotovo nakon proirenja njegovih granica, ulaze brojna stanita, koja omoguavaju ivot
vodozemaca i gmazova ekoloki potpuno razliitih potreba. Dosadanjim je istraivanjima
zabiljeeno 12 vrsta vodozemaca i 12 vrsta gmazova. Od 2014. do 2017. god. provode se prva
sustavna istraivanja sastava i distribucije faune vodozemaca i gmazova te njihove brojnosti na
pet razliitih stanita na podruju NP "Plitvika jezera". U ovom radu iznose se rezultati prve
godine istraivanja koji su dobiveni pregledom herpetolokih zbirki nekoliko prirodoslovnih
muzeja,osmatranjem,lovomiputempostavljenihtrajnihplohasaivolovkama.
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12.HBK6312 CBC
E.Kleteki,S.Leiner,M.Podnar
Naturalhistorymuseum,Zagreb,Croatia(Eduard.Kletecki@hpm.hr)
Although Plitvice Lakes National Park is among oldest protected areas of nature in Croatia,
researchofnumerousanimalgroupsthatinhabitthenationalparkisjustatthebeginning.That
impliestoamphibiansandreptilesaswell.Sporadicnotesaboutherpetofaunaofthisareaexist
since the era of AustroHungarian Empire, but complete species list does not exist and
systematicresearchhasneverbeendone.Fromthezoogeographicpointofview,Nationalpark
islocatedinaninterestingborderingareaofdistributionfornumerousspeciesofamphibians
and reptiles. With expand of its borders, the National park now includes many different
habitatswhichsupportamphibianandreptilespecieswithverydiverseecologicaldemands.So
far,wehaveevidenceof12amphibianand12reptilianspecies.Fromtheyear2014to2017
the first systematic research of composition, distribution and abundance of amphibians and
reptiles have been in progress, especially on five different habitats of Plitvice Lakes National
Park area. This paper presents results for the first year of research. Results are based on
severalmuseumherpetologicalcollectionsreviews,fieldresearch,andondatacollectedbylive
trappinginpermanentlyestablishedandcontrolledplots.
O54
ECOLOGICALLIMITATIONSANDPASTEVENTSAFFECTSPECIESDISTRIBUTION:ASSESSINGTHE
QUALITYOFSPATIALDISTRIBUTIONMODELSFORTWOREPTILESPECIES
K.vanLieshout1,S.vanStraten1,M.J.A.Weterings1,I.M.Dmmer1,D.Jeli2
1
Animal Management, Van Hall Larenstein University of Applied Sciences, Leeuwarden, The
Netherlands(koen.lieshout@hotmail.com)
2
CroatianHerpetologicalSocietyHyla,Zagreb,Croatia
Speciesdistributionmodels(SDMs)areincreasinglyimportantforconservationandalready
used for i.e. reserve planning and evaluating the effectiveness of protected areas (PAs).
However,howreliablearethesemodels?SDMsestimateaspeciesfundamentalnichewhile
the realized niche, which is the niche range that a species actually occupies, is generally
smaller than the fundamental niche due to human influences, biotic interactions and
geographic barriers. This article used two case studies (D. caspius and D. mosorensis) to
showtheimpactofthesefactorsinmodelingreptilespeciesdistributioninCroatia.MaxEnt
estimatespresenceofD.caspiusalongthecoastofCroatiaandonlotsofthesmallerislands,
however,thisspeciesisonlyfoundintheeastofCroatiaandontheislandsOlibandLastovo.
Possibleexplanationsofthisdistantdistributionareresidualpopulations,exoticspeciesand
distributionbyfloatingondebris.D.mosorensisisaspecialist,occupyingverticalhabitatson
limestone rocks and cliffs on high elevation mountaintops. The populations predicted by
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MaxEntarefragmentedduetoelevationandhorizontalhabitats.MaxEntmightthuspredict
presence in different areas but it is questionable whether this species was able to occupy
these areas due to its adaptations and poor dispersal abilities. It is thus very important to
takeintoaccountspeciesecologicallimitationsandpasteventswhenassessingthequality
ofaSDM.
O55
PRELIMINARYDATAONTHEASSESSMENTOFTHEEFFECTIVENESSOFPROTECTEDAREASFOR
TWOREPTILESPECIESUSINGSPATIALDISTRIBUTIONMODELING
K.vanLieshout1,S.vanStraten1,M.J.A.Weterings1,I.M.Dmmer1,D.Jeli2
1
Animal Management, Van Hall Larenstein University of Applied Sciences, Leeuwarden, The
Netherlands(koen.lieshout@hotmail.com)
2
CroatianHerpetologicalSocietyHyla,Zagreb,Croatia
Protected areas (PAs) should ensure the persistence of reptile species. However, several
studies already confirmed that the establishment of PAs do often not correlate with
conservation priorities. This study used a species distribution model (MaxEnt) to assess the
effectivenessofPAsinCroatiafortwodatadeficientreptilespecies(Z.situla&T.fallax).PAs
were divided in Species PAs and Landscape PAs using the IUCN Protected Areas Categories
System. The results show that the best suitable areas are not covered by species PAs
confirmingthatreptilesarenottakeninaccountduringpolicymakingandtheestablishmentof
PAs.UsingthecriterionoftheIUCNRedListCategoriesandCriteria3.1,suggestingaminimum
area requirement larger than 2.000 km2 (excluding subcategories), we could also say that
Croatiaalonecannotensurethepersistenceofthesespecies.Thisindicatestheneedforcross
nationalconservationtosustainviablepopulations.
Keywords:Protectedareas,speciesdistributionmodelling,MaxEnt,crossnationalconservation
O56
PROCJENA OSJETLJIVOSTI FAUNE VODOZEMACA I GMAZOVA U HRVATSKOJ I BOSNI I
HERCEGOVINIUZDEFINIRANJEVANIHHERPETOLOKIHPODRUJA
M.Vujanovi,D.Jeli
Hrvatskiinstitutzabiolokuraznolikost,HrvatskoherpetolokodrutvoHYLA,Zagreb,Hrvatska
(mak.vuj@gmail.com)
U Hrvatskoj i Bosni i Hercegovini ivi 60 vrsta vodozemaca i gmazova (bez morskih vrsta).
PotrebnojeprocijenitiosjetljivostsvihvrstajerprocjeneugroenostiuCrvenojknjizineuzimajuu
obzir ekoloke parametre ve samo geografsku raspodjelu. U radu je izdvojen odreen broj
varijabli koje ukljuuju ekoloke, bioloke, geografske i antropoloke imbenike. Kod pojedine
vrste za svaku varijablu upisana je jedna od etiri kategorije, od 0 najmanja, do 3 najvea
osjetljivost.Podacizasvakuvrstudobivenisupregledavanjemliterature,teizosobnihiskustava
strunjaka. Indeks osjetljivosti svake vrste izraunat je kao zbroj vrijednosti svih varijabli i
podijeljensbrojemvarijabli.Provedenajeikorelacijadobivenihindeksaosjeljivostiinacionalnog
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statusaugroenostipoCrvenojknjizi,tesudefiniranavanaherpetolokapodrujapomoutri
karte.Rezultatisupokazalidasugmazoviosjetljivijiodvodozemaca.Takoer,dobivenisuindeksi
osjetljivostikoreliranisprocjenamauCrvenojknjizi,uznekolikoiznimki.VrsteTestudohermanni,
Zamenis situla i Iberolacerta horvathi imaju veliku osjetljivost, a mali status ugroenosti koji se
treba preispitati. Vana herpetoloka podruja prostiru se po veini Hrvatske obale, te veim
dijelomDinarida.Zakljuakjedaseprocjenomosjetljivostidolazidokvalitetnijeprocjenestanja
ugroenostiherpetofaunenegoprocjenamanacionalnihstatusauCrvenojknjizi.
Kljune rijei: indeksi osjetljivosti, herpetofauna, Hrvatska, Bosna i Hercegovina, nacionalni status
ugroenosti
M.Vujanovi,D.Jeli
Croatian Institute for Biodiversity, Croatian Herpetological Society HYLA, Zagreb, Croatia
(mak.vuj@gmail.com)
Thereare60speciesofamphibiansandreptilesinCroatiaandBosniaandHerzegovina(non
marine species), so there is a need to assess their sensitivity, since the endangerment
assessmentsintheRedListtakeintoaccountonlythegeographicaldistribution,andnotthe
ecological parameters. In this paper a number of variables is singled out, which include
ecological,biological,geographical,andanthropologicalfactors.Foreachspeciesandvariable
oneoffourcategorieswasassigned:from0thelowest,to3thehighestsensitivity.Datafor
each type is obtained from examining the literature, and also from experts' personal
experiences. The sensitivity index of each species was calculated as the sum of values of all
variablesanddividedbythenumberofvariables.Acorrelationofthesensitivityindexandthe
nationalstatusofendangermentintheRedListwasmade,andimportantherpetologicalareas
were defined using three maps. The results show that reptiles are more sensitive than
amphibians. Also, the resulting sensitivity indices correlate well with the assessments in the
Red List, with few exceptions. Species Testudo hermanni, Zamenis situla and Iberolacerta
horvathiarehighlysensitive,sotheirlowendangermentstatusshouldbereviewed.Important
herpetological areas spread across most of the Croatian coast, and a large part of the
Dinarides.Inconclusion,theassessmentofsensitivitygivesbetterendangermentassessment
thanIUCNcriteria.
Key words: sensitivity index, herpetofauna, Croatia, Bosnia and Herzegovina, national
conservationstatus
O57
HABITAT REQUIREMENTS AND ABUNDANCE OF Dinarolacerta mosorensis AND D.
montenegrinaINTHEMOUNTAINSOFMONTENEGRO
K.Ljubisavljevi1,L.Polovi2,V.Ikovi1,S.Vuksanovi2,T.D.Vukov3
1
MontenegrinEcologistsSociety,Podgorica,Montenegro(ljubisavljevic.k@gmail.com)
2
TheNaturalHistoryMuseumofMontenegro,Podgorica,Montenegro
3
Department of Evolutionary Biology, Institute for Biological Research "Sinia Stankovi",
UniversityofBelgrade,Belgrade,Serbia
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12.HBK6612 CBC
Keywords:lacertidrocklizards,habitatselection,Dinaricmountains,forestfires,deforestation
O58
BIOLOGIJAPOSKOKAUKAMENOLOMUBIZEK
M.Zadravec1,2,R.Cesarec3,Z.Tadi1
1
Zavod za animalnu fiziologiju, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska
2
HrvatskoherpetolokodrutvoHYLA,Zagreb,Hrvatska(mladen.z123@gmail.com)
3
Treak6,Zagreb,Hrvatska
Poskok, Vipera ammodytes (Linnaeus, 1758) najvea je zmija otrovnica jugoistone Europe.
Meutim, neka podruja njegove biologije i dalje su nepoznata. Ovdje predstavljamo rezultate
prvog istraivanja biologije suburbanih poskoka u Hrvatskoj, od 2008. do 2014. godine, u
zatvorenomkamenolomuBizek.Ciljistraivanjabiojeutvrditipostojanjerazlikameuspolovima
uveliini,odreditikoritenjemikrostanita,podrujakretanjatemeteorolokeprilikeprikojimasu
najaktivniji. Takoer je cilj bio utvrditi prehranu, procijeniti brojnost populacije i istaknuti
posljedicenekihljudskihaktivnostinapodrujukamenoloma.Ukupnojeanalizirano140jedinki
poskoka. Utvreno je da postoje razlike u veliini meu spolovima, a oba spola pokazuju vrlo
sline sklonosti prema vrsti mikrostanita i vremenskim uvjetima, osim u vlazi zraka. Meu
spolovimanemarazlikeudnevnojaktivnosti,aliimausezonskoj.Nemanimeuspolnihrazlikau
prehrani.Odraslimujacisekreunajveimpodrujem.ienjekamenolomaimajasannegativni
utjecajnapopulaciju,akoseizvodinakrivinainiukrivodobagodine.Rezultatiovogistraivanja
dajuuvidunekedijelovebiologijeposkokaukamenolomutemogukoristitizaposlenicimaParka
prirodeMedvednicauupravljanjutimprostorom.
Kljunerijei:Viperaammodytes,suburbanapopulacija
BIOLOGYOFTHENOSEHORNEDVIPERINTHEBIZEKQUARRY
M.Zadravec1,2,R.Cesarec3,Z.Tadi1
1
DepartmentofAnimalPhysiology,FacultyofScience,UniversityofZagreb,Zagreb,Croatia
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12.HBK6712 CBC
CroatianHerpetologicalSocietyHYLA,Zagreb,Croatia(mladen.z123@gmail.com)
Treak6,Zagreb,Croatia
TheNosehornedViper,Viperaammodytes(Linnaeus,1758)isthelargestvenomoussnake
inthewesternBalkans.However,there'spaucityofresearchinsomeaspectsofitsbiology.
Here we present the results of the first study of the biology of suburban V. ammodytes in
Croatia, from 2008 till 2014, in the inactive Bizek quarry. Our goals were to determine the
existenceofsexualsizedimorphism,microhabitatuse,homerangesize,weatherconditions
duringpeakactivity,foodcompositionandpopulationsize.Wealsowantedraiseawareness
abouttheinfluenceofsomehumanactivitiesinthequarryontheviperpopulation.Atotal
of 140 individuals were analyzed. Sexual size dimorphism was confirmed, and both sexes
showverysimilarpreferencesinmicrohabitatuseandweatherconditions,exceptinrelative
humidity. There is no difference between the sexes in daily act ivity, but there are
differences in seasonal activity. There are no differences in the diet composition between
the sexes. Adult males have the largest home ranges. The cleaning activities in the quarry
have a clear negative impact on the population, if done incorrectly and in the wrongtime.
TheresultsofthisresearchgiveanewviewonsomeaspectsofthebiologyofNosehorned
vipers in the Bizek quarry and can be of use to the Nature Park Medvednica staff in
managementofthearea.
Keywords:Viperaammodytes,suburbanpopulation
O59
NEWDATAANDDISTRIBUTIONOFCOMMONSPADEFOOTTOADPelobatesfuscus(Laurenti,
1768)INWESTERNBALKANS
A.uri1,A.Zimi2,D.Jeli3
1
Society for Research and Protection of Biodiversity, Banja Luka, Bosnia and Herzegovina
(anna.curic@hotmail.com)
2
HerpetologicalAssociationinBosniaandHerzegovinaATRA,Sarajevo,BosniaandHerzegovina
3
CroatianInstituteforBiodiversity,CroatianHerpetologicalSocietyHyla,Zagreb,Croatia
Basedonknownliteraturedataanddatacollectedduringfieldinvestigationsin2014and2015,
we present updated distribution map of Common spadefoot toad (Pelobates fuscus) in
WesternBalkans(BosniaandHerzegovina(BH)andCroatia).PelobatesfuscusislistedasLCon
the IUCN Red List and DD in Croatian Red Book, and the species is in constant population
decline.Untilyear2014thisspecieswasonlysuspectedtoinhabitBH.Todaywehaveseveral
positivelocalitiesinPosavinaregionpresentedwithpreciselocality,elevationandcoordinates.
InBHnewpopulationsarefoundneartownsofSrbac,Brod,Modriaandamac.Distribution
map includes east part of Lijeve polje and whole Posavina and there is a big chance that
species range extends also through Semberija. In this paper we also present first findings of
tadpoles, more precisely, the reproductive sites of Common spadefoot toad in the BH. In
Croatia this species is found along the rivers Mura, Drava and Sava, including most lowland
areasupto300mabovesealevel.NewlocalitiesinCroatiaareconfirmedinIvanovoseloand
Bjelovar,connectingtheDravaandSavaRiverpopulations.
Keywords:conservation,decline,Posavina,PannonianPlain
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O60
KOMPARATIVNA MORFOLOGIJA, EKOLOGIJA I ANALIZA STRUKTURE HRVATSKE POPULACIJE
PelophylaxesculentusKOMPLEKSA
K.avlovi1,D.Karaica2,I.Buj3,V.MietiStankovi4,L.Choleva5,D.Jeli6
1
Zavod za humanu reprodukciju, Klinika za ginekologiju i porodnitvo, Kliniki Bolniki Centar
Rijeka,Rijeka,Hrvatska(ivf.rijeka@gmail.com)
2
Jedinica za molekulsku toksikologiju, Institut za medicinska istraivanja i medicine rada,
Zagreb,Hrvatska
3
Zoologijski zavod, Prirodoslovno matematikog fakulteta, Sveuilite u Zagrebu, Zagreb,
Hrvatska
4
Hrvatskiprirodoslovnimuzej,Zagreb,Hrvatska
5
Institutzaanimalnufiziologijuigenetiku,AkademijaznanostiRepublikeekeBrno,Republika
eka
6
Hrvatski institut za bioloku raznolikost, Hrvatsko herpetoloko drutvo HYLA, Zagreb,
Hrvatska
K.avlovi1,D.Karaica2,I.Buj3,V.MietiStankovi4,L.Choleva5,D.Jeli6
1
Divisionforhumanreproduction,DepartmentofObstetricsandGynecology,ClinicalHospital
CenterRijeka,Rijeka,Croatia(ivf.rijeka@gmail.com)
2
Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Zagreb,
Croatia
3
DepartmentofZoology,FacultyofScience,UniversityofZagreb,Zagreb,Croatia
4
CroatianNaturalHistoryMuseum,Zagreb,Croatia
5
InstituteofAnimalPhysiologyandGenetics,AcademyofSciencesoftheCzechRepublic,Brno,
CzechRepublic
6
CroatianInstituteforBiodiversity,CroatianHerpetologicalSocietyHYLA,Zagreb,Croatia
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One of the most complicated and evolutionary intriguing animal hemiclonal systems is the
Pelophylax esculentus hybridogenetic complex which includes Pelophylax ridibundus (R), P.
lessonae (L), and P. kl. esculentus (E) species. Previous studies have analyzed population
structure,diet,reproductivemodesanddistributionofPelophylaxesculentuscomplexspecies
but similar data on the rarely observed REL populations are still scarce. This study aims to
reveal population structure, morphology and diet of the P. esculentus complex population
locatedinthecontinentalpartofCroatia.Weusedmorphologicaltraitsandallozymeanalysis
for species identification of randomlycollected93green frog individuals. Also,wecompared
diet, age and growth index profiles of species present in this population by respectively
analyzingtheirstomachcontentandestimatingthenumberoflinesofarrestedgrowthvisible
inthecrosssectionsoffemurbonesusingskeletochronology.Resultsrevealedthepresenceof
RELpopulationwithP.ridibundusrepresentingthelowestnumberofindividuals.Despitegreat
morphological interspecific variability, we observed some P. ridibundus and P. kl. esculentus
individualsexpressingrareanduniquecolorationfeatures.DietandannualgrowthrateofP.
ridibundus individuals differed from other species indicating possibly evolutionary important
specificspeciesspeciesinteractionsinthestudiedRELpopulationtype.
Key words: Pelophylax esculentus complex, morphology, allozyme analysis, food diet,
skeletochronology
O61
MOLEKULARNA ISTRAIVANJA ZELENIH ABA (ROD Pelophylax) U HRVATSKOJ
PRELIMINARNIREZULTATI
M.Jeli1,M.Vuci1,G.Klobuar1,P.Korlevi2,D.iki1,D.Franjevi1,D.Jeli3
1
Sveuilite u Zagrebu, Prirodoslovnomatematiki fakultet, Bioloki odsjek, Zoologijski zavod,
Zagreb,Hrvatska(misel.jelic@biol.pmf.hr)
2
Max Planck Institute for Evolutionary Anthropology, Department of Evolutionary Genetics,
Leipzig,Njemaka
3
Hrvatskiinstitutzabiolokuraznolikost,HrvatskoherpetolokodrutvoHyla,Zagreb,Hrvatska
Prema literaturnim podacima, na podruju Hrvatske juno od rijeke Save obitava vrsta
Pelophylaxridibundus,doksesjevernijeodSavemogupronaivrsteP.ridibundus,Pelophylax
lessonae te njihov hibridni kompleks (Pelophylax kl. esculentus). Budui da je morfoloko
odreivanje vrsta i hibrida zelenih aba prilino nepouzdano, cilj ovog istraivanja bio je
odredititaksonomskistatuszelenihabauHrvatskojupotrebommolekularnihbiljega(analiza
varijacijeduljineserumalbumingenintronske1regijeianalizamitohondrijskoggenazaNADH
dehidrogenaznu podjedinicu 3). Istraivanje je provedeno na populacijama zelenih aba iz
razliitih dijelova Hrvatske (ribnjaci Crna Mlaka, Lika, Istra i Dalmacija). Rezultati su potvrdili
prisutnostvrstaP.ridibundus,P.lessonaeisnjimapovezanogkleptonaP.kl.esculentus,alisu
pokazali neoekivanu prisutnost vrste Pelophylax kurtmuelleri te novih neopisanih hibridnih
kompleksa.PrisutnostvrsteP.kurtmuelleriuLiciinaribnjacimaCrnaMlaka,tenalazvrsteP.
lessonae na planini Potak (Dalmacija), ukazuju na nunost revizije podataka o
rasprostranjenostizelenihabauHrvatskoj.
Kljunerijei:zapadniBalkan,P.kurtmuelleri,serumalbuminintron1regija,mitohondrijskaregija
ND3
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AccordingtoliteraturedataonthedistributionofwaterfrogsinCroatia,theareasouthofthe
SavaRiverisinhabitedbyPelophylaxridibundus,whileP.ridibundus,Pelophylaxlessonaeand
theirhybridogeneticcomplex(Pelophylaxkl.esculentus)canbeobservedintheareanorthof
theSavaRiver.Sincethemorphologicaldeterminationofspeciesandhybridsofwaterfrogsis
notreliable,the aimofthisstudywastodeterminethetaxonomicalstatusofwaterfrogsin
Croatia using molecular markers (length variation of serum albumin gene intron1 and the
mitochondrialgene for NADH dehydrogenase subunit 3).The studywas performed on water
frogpopulationsfromnorthernpartandAdriaticcoastofCroatia(CrnaMlakafishponds,Lika,
Istra,Dalmatia,).ResultsconfirmthepresenceofspeciesP.ridibundus,P.lessonaeandtheir
associatedkleptonP.kl.esculentus,andshowanunexpectedpresenceofspeciesPelophylax
kurtmuelleriaswellasnewhybridcomplexes.ThepresenceofP.kurtmuelleriintheLikaregion
andCrnaMlakafishponds,andthediscoveryofP.lessonaeinthePotakMountain(Dalmatia),
pointtotheimportanceofrevisingthedistributiondataofwaterfrogpopulationsinCroatia.
Key words: Western Balkans, P. kurtmuelleri, serum albumin gene intron1, mitochondrial
regionND3
O62
PROCJENA STAROSTI JEDINKI ZELENIH ABA RODA Pelophylax (FITZINGER, 1843) IZ
POPULACIJESASKADARSKOGJEZERA,CRNAGORA
T.Voloen1,B.Prakljai2,D.Karaica3,D.Jeli4,5
1
Nastavni zavod za javno zdravstvo "Dr. Andrija tampar", Zagreb, Hrvatska
(tatjana2112@gmail.com)
2
CentarzazatituiprouavanjepticaCrneGore,Podgorica,CrnaGora
3
Jedinica za molekulsku toksikologiju, Institut za medicinska istraivanja i medicinu rada,
Zagreb,Hrvatska
4
Hrvatskiinstitutzabiolokuraznolikost,Zagreb,Hrvatska
5
HrvatskoherpetolokodrutvoHYLA,Zagreb,Hrvatska
NapodrujuSkadarskogjezera,pretpostavljenojedatrirazliitataksonarodaPelophylaxine
jedinstvenu populaciju slinu ve opisanim populacijama kompleksa Pelophylax esculentus.
Budui da roditeljske vrste ove populacije pripadaju vrstama P. kurtmuelleri i P. shqipericus,
ovoje,premanaemsaznanju,prviizvjetajnovog,joneopisanogkompleksazelenihaba.Cilj
rada je otkriti dobnu strukturu populacije zelenih aba sa Skadarskog jezera (Crna Gora)
procijenjujui i usporeujui prosjenu starost i brzinu rasta svih prisutnih taksona. Iz
spomenute populacije, ukupno 83 jedinke zelenih aba su runo ulovljene tijekom
dvogodinjeg perioda (2011. i 2012. godine). Nakon pravilne eutanazije, iz svih ulovljenih
jedinkiizdvojenesubedrenekosti.Metodomskeletokronologijekostisupotompripremljenei
histolokianaliziranekakobiseprepoznavanjemlinijausporenograstamoglaprocijenitidob
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pojedine jedinke. Prema procjeni, najmlaa jedinka je imala jednu, a najstarija 6 godina.
Prosjena procijenjena dobroditeljske vrste P. kurtmuelleri (4god.) bitno se razlikuje od one
procjenjenezaP.shqipericus(1,5god.)ihibridnuvrstu(1,8god.).Zanimljivo,pokazanojedase
brzina rasta razlikuje samo izmeu P. shqipericus i hibridne vrste. Otkrivene razlike u
procijenjenoj prosjenoj dobi i brzini rasta spomenutih vrsta mogle bi odraavati postojanje
specifinih interakcija meu vrstama koje posljedino uvjetuju strukturu ove zanimljive
populacijekompleksaPelophylax.
T.Voloen1,B.Prakljai2,D.Karaica3,D.Jeli4,5
1
AndrijatamparTeachingInstituteofPublicHealth,Zagreb,Croatia(tatjana2112@gmail.com)
2
CenterforProtectionandResearchofMontenegro,Podgorica,Montenegro
3
Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Zagreb,
Croatia
4
CroatianInstituteforBiodiversity,Zagreb,Croatia
5
CroatianHerpetologicalSocietyHyla,Zagreb,Croatia
AttheLakeSkadar,itisassumedthatthreetaxaofgreenfrogsformasinglePelophylaxgenus
population similar to already known Pelophylax esculentus complex populations. Since the
parentspecies belong to P. kurtmuelleri and P. shqipericus, this isto ourknowledge the first
reportofanewandyetundescribedgreenfrogcomplex.Theaimthestudywastorevealthe
populationagestructurebyestimatingandcomparingtheaverageageandgrowthratesofall
taxa present in this new Pelophylax complex population. In total, 83 green frog individuals
werecollectedbyhandduringthetwoyearperiod(2011and2012)atthesiteofLakeSkadar
(Montenegro).Afterpropereuthanasia,femurboneswereisolatedfromallcollectedanimals.
Usingtheskeletochronologymethodtheboneswerepreparedandhistologicallyanalyzedfor
theexistenceoflinesofarrestedgrowthwhichallowedtheageestimationofeachindividual.
The youngest animal was estimated to have one whereas the oldest had 6 year of age. The
estimatedaverageageofparentalspeciesP.kurtmuelleri(4years)wassignificantlyhigherthan
thatofP.shqipericus(1.5years)andtheirhybrid(1.8years)species.Interestingly,thegrowth
rate differed only between P. shqipericus and the hybrid species. In conclusion, detected
differences in the average age and growth rates of these species could mirror the specific
interspecies interactions shaping the structure of this interesting Pelophylax complex
population.
O63
ODREIVANJE VRSTA I HIBRIDA ZELENIH ABA (ROD Pelophylax) SA SKADARSKOG JEZERA
(CRNAGORA)ANALIZOMMOLEKULARNIHBILJEGA
M.Vuci1,M.Jeli1,B.Prakljai2,G.Klobuar1,D.Jeli3
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12.HBK7212 CBC
Zapadnopalearktikezeleneabe(rodPelophylax)jeiznimnosloenrodukojemjetrenutno
opisano dvadesetak vrsta i tri hibridna kompleksa.Danas se zelene abe intenzivno istrauju,
ponajprijezbogpostojanjahibridnihkompleksashemiklonalnimnainomrazmnoavanja.Iako
prema literaturnim podacima na Balkanskom poluotoku nije dokazano postojanje hibridnih
kompleksa, preliminarna istraivanja morfolokih osobina zelenih aba sa Skadarskog jezera
(CrnaGora)ukazujudaoniipakpostoje.Buduidaodreivanjavrstaihibridazelenihabana
temeljumorfolokihosobinanijepouzdanoonojeuovomistraivanjuprovedenokoritenjem
molekularnih biljega. Istraivanje je provedeno na 100 uzoraka zelenih aba sa Skadarskog
jezera, koji su analizirani upotrebom jezgrinog biljega (serum albumin intron1 regija) i
mitohondrijskog biljega (gen za NADH dehidrogenaza podjedinicu 3). Rezultati su pokazali
prisutnostvrstaPelophylaxkurtmuelleriiPelophylaxshqipericus,teunajveembroju,novog,
dosadneopisanoghibridnogkompleksa.UovomistraivanjunisuzabiljeenevrstePelophylax
ridibundus, Pelophylax lessonae i njihov hibrid Pelophylax kl. esculentus, iako je njihova
prisutnostbilamoguapremaliteraturnimnavodima.
Kljunerijei:Balkanskipoluotok,P.kurtmuelleri,P.shqipericus,serumalbuminintron1regija,
mitohondrijskaregijaND3
M.Vuci1,M.Jeli1,B.Prakljai2,G.Klobuar1,D.Jeli3
1
UniversityofZagreb,FacultyofScience, Department ofBiology,DivisionofZoology,Zagreb,
Croatia(matej.vucic@biol.pmf.hr)
2
CenterforprotectionandresearchofMontenegro,Podgorica,Montenegro
3
CroatianInstituteforBiodiversity,CroatianHerpetologicalSocietyHYLA,Zagreb,Croatia
Western Palearctic water frogs (genus Pelophylax) is extremely complex genus with
approximately twelve species and three hybrid complexes. Today, water frogs are being
intensively studied, primarily because of the presence of hybrid complexes with hemiclonal
strategyofreproduction.Although,accordingtoliterature,theexistenceofhybridcomplexeshas
not been proven on the Balkan Peninsula, preliminary research into the morphological
characteristics of water frogs from the Skadar Lake (Montenegro) indicate that they do exist.
Since the determination of species and hybrids of water frogs on the basis of morphological
featuresisnotreliable,theaimofthisstudywastoanalysethewaterfrogsfromtheSkadarLake
usingmolecularmarkers.Thestudywasconductedon100samplesofwaterfrogs,whichwere
analysedusingnuclearmarker(serumalbuminintron1)andmitochondrialmarker(geneNADH
dehydrogenase subunit 3). The results showed the presence of species Pelophylax kurtmuelleri
and Pelophylax shqipericus, and mostly, a new, up to now undescribed hybrid complex. In this
study, species Pelophylax ridibundus, Pelophylax lessonae and their hybrid Pelophylax kl.
esculentuswerenotrecorded,althoughtheirpresencewaspossibleaccordingtotheliterature.
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O64
THREATENINGLEVELSANDEXTINCTIONRISKSBASEDONDISTRIBUTIONAL,ECOLOGICALAND
LIFEHISTORYDATASETS(DELH)VERSUSIUCNCRITERIAEXAMPLEOFSERBIANREPTILES
Lj.Tomovi1,2,A.Uroevi2,T.D.Vukov2,R.Ajti3,K.Ljubisavljevi2,I.Krizmani1,D.Jovi4,N.
Labus5,S.orevi1,M.L.Kalezi2,G.Duki2,L.Luiselli6,7
1
UniversityofBelgrade,FacultyofBiology,Belgrade,Serbia(lili@bio.bg.ac.rs)
2
UniversityofBelgrade,InstituteforBiologicalResearchSiniaStankovi,Belgrade,Serbia
3
InstituteforNatureConservationofSerbia,Belgrade,Serbia
4
InstituteforNatureConservationofSerbia,Ni,Serbia
5
Biology Department, Faculty of Science and Mathematics, University of Pritina, Kosovska
Mitrovica,Serbia
6
Niger Delta Ecology and Biodiversity Conservation Unit, Department of Applied and Environmental
Biology,RiversStateUniversityofScienceandTechnology,PortHarcourt,RiversState,Nigeria
7
CentreofEnvironmentalStudiesDemetra,Rome,Italy
Recentstudiesontheworldreptilesextinctionriskspointedoutthatabout20%ofspecies
are threatened, with another 20% are datadeficient following IUCN criteria. IUCN set up
criteriaforassessmentofextinctionriskatglobalandregionalscales,relyingondistribution
data, population parameters and quantitative analyses. Only 12% of reptilian species were
listedasgloballythreateneduponpopulationdata.Longtermpopulationstudiesaretime
consuming, thus it would be practical to use some other parameters for estimations of
extinctionrisks.Threateninglevel(andextinctionrisks)ofSerbianreptileshaveneverbeen
investigated. We aimed to scrutinize species threatening level (thus set up conservation
priorities) on the basis of distributional, ecological and lifehistory datasets (DELH). We
estimated which traits are the most important for the conservation of species. Separately,
weusedIUCNcriteriaforregionalleveltoassessextinctionriskandcomparedthemwiththe
results obtained by analyses of DELH datasets. The two types of criteria gave similar
estimates for the chelonian species. In snakes, two species exhibited higher conservation
scores by DELH than by IUCN criteria, the opposite being true for two other species. In
lizards,onlytwospecieshadhigherextinctionrisksbyIUCNwhereasfivelizardsweremore
threatenedaccordingtoDELH.OurintentionwasnotdiscreditthevaluesofIUCNcriteriafor
settingregional(ornational)extinction.
Keywords:range,naturalhistory,redlisting,threatenedcategories
O65
HEMIPENIALMORPHOLOGYASAUSEFULCOMPLEMENTARYTOOLSUPPORTINGTHEFULL
SPECIESSTATUSOFCLOSELYRELATEDSCINCIDLIZARDS(SCINCIDAE,SAURIA,SQUAMATA)
V.Vergilov1,N.Tzankov2,B.Zlatkov1
1
Department of Zoology andAnthropology, Faculty of Biology, Sofia University St. Kliment
Ohridski,Sofia,Bulgaria(vladislav8807@gmail.com)
2
DepartmentofVertebrates,NationalMuseumofNaturalHistoryBAS,Sofia,Bulgaria
Despite the great species diversity of the scincid, few useful determinative morphological
characteristics have been evaluated, related mainly to pholidotic and osteological
characters. Hemipenial morphology has been widely used in squamate interspecific
reptilestaxonomy. The currently available data for about 3% of the total scincid diversity
renders this topic widely unexplored. Here we present the first description of hemipenial
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morphologyofthecloselyrelatedinphylogeneticsenseAblepharuskitaibeliiandA.budaki.
Weadoptedtechniquesdevelopedforeversionandstainingofendophallusoflargemoths
instead of the classical techniques. The shape of the everted organs closely resembled the
sphenomorphineskinks,beingdeeplybifurcatedwithrelativelylongbranches.Inbothtaxa
terminal awls were observed and no calcified but minute keratin microscopic structures
were present with varying density from the top to the base of the organ. Besides its
generallymoreelongatedshapeandbranches,uniqueleafshapedfoldsnamedauriculiwere
also observed on both sides of the body in A. budaki. The differences between the
hemipenes of both taxawere substantial and revealed that they differed by more essential
specific morphological characters, than those mentioned in already published sources.
Resultscouldserveasacomplementarytoolinsupporttotheirfullspeciesstatus.
Keywords:Ablepharus,hemipenis,interspecificdifferentiation,folds
O66
EXSITU DRANJE AUTOHTONIH VODOZEMACA I GMAZOVA U ZOOLOKOM VRTU GRADA
ZAGREBAUSVRHUEDUKACIJE,ZATITEIISTRAIVANJA
I.Cizelj1,M.Luka2,D.Jeli3,I.Perani1
1
UstanovaZoolokivrtgradaZagreba,Zagreb,Hrvatska(ivan@zoo.hr)
2
Zavodzabolestiperadisklinikom,Veterinarskifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
3
Hrvatskiinstitutzabioraznolikost,Zagreb,Hrvatska
Exsitudranjeivotinjaomoguavaprikupljanjevelikogbrojapodatakakojejevrlotekoprikupiti
u prirodnom stanitu. Zooloki vrtovi imaju veliko iskustvo u dranju i brizi velikog broja
raznovrsnih vrsta ivotinja. Takoer, esto imaju raspoloiv prostor, dostupnost raznovrsne
prehrane potrebne ivotinjama te stalan nadzor i skrb za ivotinje. Drane ivotinje mogu se
koristititizabrojnabiolokaiveterinarskaistraivanja,tezaedukacijujavnostidirektnimputemili
implementacijomuedukativneprogramezadjecu.ZoolokivrtgradaZagrebatijekomposljednjih
godinasudjelovaojeudranjuiistraivanjuvelikogbrojaautohtonihvodozemacaigmazovakao
to su planinski utokrug (Vipera ursinii macrops), riovka (Vipera berus), rijena kornjaa
(Mauremys rivulata), ovjeja ribica (Proteus anguinus), ivanjski rova (Ablepharus kitaibelii) i
mnogi drugi. Spomenuta istraivanja odnosila su se primarno na ivotni ciklus, reproduktivnu
biologiju, inkubaciju jaja, morfometriju mladunaca i njihovu prehranu kao i na parazitoloka i
mikrobiolokaistraivanja.Prikupljanjempodatakaoivotnomciklusu,prehraniirazmnoavanju
autohtonih vodozemaca i gmazova mogue je poboljati mjere zatite u prirodi te pritom
educirativelikibrojjavnosti.Usluajupotrebe,pomouprikupljenihsaznanja,moguejeosnovati
ipopulacijekritinougroenihvrstazareintrodukcijuubudunosti.
Kljunerijei:vodozemci,gmazovi,zatita,edukacija,istraivanje
EXSITUKEEPINGOFNATIVEAMPHIBIANSANDREPTILESINZAGREBZOOFOREDUCATION,
CONSERVATIONANDRESEARCHPURPOSES
I.Cizelj1,M.Luka2,D.Jeli3,I.Perani1
1
ZagrebZoo,Zagreb,Croatia(ivan@zoo.hr)
2
Department of Poultry Diseases with Clinic, Faculty of Veterinary Medicine, University of
Zagreb,Zagreb,Croatia
3
CroatianInstituteforBiodiversity,Zagreb,Croatia
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Exsitu animal keeping allows collecting high number of data that is difficult to collect in
nature.Zoologicalgardenshavealotofknowledgeinkeepingvarietyofanimalspeciesand
their husbandry. Also, they have space available, proper food and constant surveillance of
animals. Animals in captivity can be used for different kind of biological and veterinary
research, and also for education of public directly, or by implementing certain species in
educationprogramsforkids.Duringlastyears,Zagreb Zoohas beentakingpartinkeeping
and research of many native amphibian and reptile species, like Karst viper (Vipera ursinii
macrops), Adder (Vipera berus), Olm (Proteus anguinus), Balkan Terrapin (Mauremys
rivulata), Snakeeyed skink (Ablepharus kitaibelii) and many other species. Research is
focused mostly on life cycle, reproductive biology, egg incubation, morphometry of young
animals and their nutrition, but also on parasitology and microbiology research. Collecting
data about life cycle, nutrition and reproduction of native amphibian and reptile species
allows more efficient conservation measurements as well as raising of public awareness. If
needed, gained knowledge might be used for establishing populations of critically
endangerednativespeciesforfuturereintroduction.
Keywords:amphibians,reptiles,conservation,education,research
O67
IWASANINJAATYOURAGESAIDANELDERLYTORTOISETOAHATCHLINGONTOGENY
SHIFTINAGILITY
A.Golubovi
Institute of Zoology, Faculty of Biology, University of Belgrade, Belgrade, Serbia
(golubovic.ana@bio.bg.ac.rs)
Tortoises rigid shells protect soft body parts, but also disable flexion of torso, thus
negatively affect their agility. For example, tortoises have problems overcoming inclined
obstacles, and selfrighting when overturned on their dorsum. Juvenile tortoises are
surroundedwithrelativelylargerobstaclesthanadultsfromthesamehabitat,thusaremore
challenged during everyday movements. To explore change in agility during ontogeny I did
two tests on wild running Hermanns tortoises (Testudo hermanni). First experiment (149
examinees) explored how fast tortoises can overcome 50 cm high negative steep step. I
compared juveniles and adults from two localities. Although adults needed longer time to
startmoving,theycrossedtheobstaclefarquickerthanjuvenilesatbothlocalities.Second
experiment (338 examinees) tested differences in selfrighting behavior between juveniles
and adults from three populations. Again juveniles started moving after shorter period of
time, but also overturned quicker and were more successful to finish the task.Both
experiments demonstrated fast reaction in juvenile tortoises. This could be result of
predator avoidance response. Soft, barely ossified shells of juveniles do not offer efficient
protection from wide range of predators. Secretive lifestyle could be their main anti
predatorresponse,thuspromptreactionandefficientmovingfromconspicuousplacecould
haveavitalimportanceforyoungtortoises.
Keywords:Tortoise,Agility,Behaviour,Ontogeny
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O68
COMPARATIVEMORPHOLOGYOFSalamandraatraprenjensis(BOSNIAANDHERZEGOVINA)
WITHITSNOMINOTYPICSUBSPECIESSalamandraatraatra(AUSTRIA)
E.unje1,2,D.Jeli3,M.Mller4,R.krijelj1,V.Helfer5
1
FacultyofNaturalScience,Sarajevo,BosniaandHercegovina(sunje.emina@gmail.com)
2
HerpetologicalAssociationofBosniaandHerzegovina:ATRA(BHHU:ATRA),Sarajevo,Bosnia
andHerzegovina
3
CroatianHerpetologicalSociety:Hyla,Zagreb,Croatia
4
UniversityofSalzburg,Salzburg,Austria
5
LeibnizCenterforMarineTropicalEcology,Bremen,Germany
Miki(1969)describedthesubspeciesSalamandraatraprenjensis,endemictoDinarides.The
subspecies status has been debated. Because of discontinuous distribution from nominal
subspecies(Salamandraatraatra)anddifferentecosystemstheyinhabit,thisstudyaimedto
detectdevelopmentalchangesinformandcovariancebetweenecologicalfactorsandshape,
byusingtwosubpopulationsinAustria(Austrianpopulation)andtwosubpopulationsinBosnia
andHerzegovina(B&Hpopulation).14quantitativecharacteristicsweremeasuredfor60adult
individuals from Austria and 195 adult individuals from B&H. In comparison Austrian vs B&H
population, significant difference between all characteristics besides Body mass, TL, SL and
Distance between legs is registered. Same results appear when comparing intrasex variation
between populations from different countries, where Head length differed only between
femalesandbodymassonlybetweenmales.Mostsignificantintersexdifferenceingeneralwas
found in length of front and hind legs, which is closely related to the copulation act phases.
AustrianpopulationexpressessignificantintersexdifferencesinallcharacteristicsbesidesNb.
ofcostalgrooves,TL,SL,Taillength,HeadandJawlength.FemaleswithinBosnianpopulation
significantly differ from males in characteristics related to size of feet, hands and limbs.
Analyzes were performed using ANOVA. Results contributed in deducing conclusions on
functionandevolutionaryrelationshipsbetweenthesetwosubspecies.
Keywords:Comparativeevolution,morphometrics,amphibians
O69
APPLICATION OF MICRO CT SCANNING AND 3D GEOMETRIC MORPHOMETRICS IN
EXPLORINGEVOLUTIONOFSKULLSHAPEINTriturusNEWTS(CAUDATA:SALAMANDRIDAE)
A.Ivanovi1,J.W.Arntzen2
1
Institute of Zoology, Faculty of Biology, University of Belgrade, Belgrade, Serbia
(ana@bio.bg.ac.rs)
2
NaturalisBiodiversityCenter,Leiden,TheNetherlands
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distribution of the species in the morphological space indicate that evolutionary changes in
skullshapewererelatedtoevolutionarychangesinskullsize(evolutionaryallometry).Also,in
thisgroup,changesinskullshapeappeartobehighlycorrelatedtothebodyelongation,and
changesinthenumberofvertebrae.
Keywords:microtomography,geometricmorphometrics,cranium,allometry
O70
DATAABOUTSTRIPEDNECKEDTERRAPIN,MauremysrivulataINVLORABAY,ALBANIA
E.Sadanaku1,I.Haxhiu2
1
Department of Biology, University of "Ismail Qemali" Vlora, Vlore, Albania
(eneriti@gmail.com)
2
HeadofAlbanianHerpetologicalAlbanianSociety(HAS),Tirana,Albania
Thisstudyaimstoprovideinformationaboutmorphometrics,populationstructureandbiologyof
the Stripednecked Terrapin, Mauremys rivulata in the area of Vlora Bay, Albania. Since the
studies and publications in Albania are very scarce, this study has a highly importance in
understandingthebioecologyofthisspecies.Twomaindifferenthabitats,freshwaterchannles
(severalchannelsindifferentareas)andponds orswamps(twopondsindifferentareas)were
monitoredfromApril2013toMay2015.AhighnumberofMauremysrivulatawereobservedin
thewateroroutofthewater(basking)inalldifferenthabitats,where27werecapturedforthe
firsttimeand1wascapturedforthesecondtime(recapture).Thelargestnumberofindividuals
ofM.rivulatawereobservedinZvernecpond[4003039.19N1902427.43E]andinfreshwater
channel of Panaja [4003446.86N 1902820.40E], while very few individuals were observed in
Orikumi pond [4001926.87N 1902709.56E]. All captured individuals were studied using
differentmethodologies.CurvedCarapaceLength(CCL),CurvedCarapaceWidth(CCW),Plastron
Length(PL),Plastronwidth(PW)andbodysizeweremeasuredforeachindividualofMauremys
rivulataandwerestatisticallyanalyzed.EachadultindividualofMauremysrivulatawasmarked
by notching the marginal scutes of carapace. Sex was determined by secondary sexual
characteristics(e.g.,plastralconcavity,lengthoftail)andresultedthat14.8%weremales,33.4%
females and 51.8% juveniles. The presence of Mauremys rivulatain the area of Vlora Bay is an
importantfinding,becauseitisconsideredasararespeciesinAlbania.
Keywords:Albania,female,juvenile,habitat,male,Mauremysrivulata,VloraBay
O71
MODELING THE POTENTIAL DISTRIBUTION OF THE SPURTHIGHED TORTOISE (Testudo
graeca) AND THE HERMANNS TORTOISE (T. hermanni) AT THE NORTHERN LIMIT OF THEIR
RANGEINROMANIA
C.Samoila1,D.Cogalniceanu1,I.Ruben1,M.Bogdan1,A.Dumitrescu2
1
Faculty of Natural and Agricultural Sciences, Ovidius University of Constanta, Constanta,
Romania(ciprian.samoila@stiintelenaturii.ro)
1
AsociatiaCheloniaRomania,Bucuresti,Romania
2
DepartmentofClimatology,NationalMeteorologicalAdministration,Bucharest,Romania
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12.HBK7812 CBC
The two species of tortoises reach the limit of their northernmost range in Romania and we
testediftheirrangeswererestrictedbygeographicorclimaticbarriers.Ourstudyfocusedon
the entire range of the two tortoises in Romania. We ran the Maximum Entropy algorithm
(MaxEnt) to model the current potential distribution using presenceonly occurrence records
(1321recordsforT.graecaand767forT.hermanni)andaselectionofhighresolutionclimatic
surfaces derived from the national network of weather stations in Romania. T. graeca is
widespread in Dobrogea region except from the Danube Delta due either to the barrier
representedbytheriverortothehighmortalityinducedbytheperiodicfloods.T.hermanni
has a narrow range limited to the southernmost areas of Romania and seems to be mostly
affected by precipitation of the driest month and minimum temperatures of coldest month.
TheSpurthighedtortoiseisclearlylimitedgeographicallybytheDanuberiversincethereisno
occurrence outside Dobrogea region, and the Hermanns tortoise range seems limited by
extremetemperaturevaluesduringthewinter.
Keywords:Testudo,graeca,hermanni,MaxEnt,speciesdistributionmodeling
O72
DETECTIONOFProteuseDNAINKARSTGROUNDWATER
DuetoinaccessiblesubterraneanhabitatofProteusanguinus,itspresencecanonlyrarelybe
confirmedbyclassicalsurveymethodssuchastrappingandvisualencounters.Toovercome
this problem, we employed environmental DNA (eDNA) method using a SYBR chemistry
based realtime PCR assay with two sets of specific primers to amplify short mitochondrial
DNA sequences in the 16S rDNA gene (153 bp) and in the control region (106 bp). The
specificity of the assay was previously tested on trout, crested newt and human DNA. In
controlledconditionsattheTularCaveLaboratorytheminimumdensityofProteusatwhich
its DNA could still be detected corresponded to one animal per 256 m3 of standing water,
whensampling20Lofwater.Themethod,testedatthreeSlovenianfieldtestsitesoccupied
by different lineages of Proteus (the springs of Vir pri Stini and Mahnii, and the cave
Kompoljska jama), was 100% effective. Subsequently, a pilot survey of Proteus distribution
wasconductedalongthesouthernlimitofitsknownrangeinHerzegovinaandMontenegro.
UsingDNAbasedidentificationweunequivocallyestablishedthepresenceofProteusatfour
sites,andfounditslikelytracesatadditionaleightsitesallnewlocalitiesforthisspecies.
By applying eDNA method to Proteus, we developed innovative noninvasive approach for
reliableandefficientmonitoringofthespeciesandpossiblyforotheraquaticcavedwelling
organisms.
Keywords:environmentalDNA,realtimePCR,distribution,noninvasivesampling,Proteus
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O73
ZNAAJKEPOPULACIJERIJENEKORNJAE,MauremysrivulataIZKONAVOSKOGPOLJA
A.tih,I.Buri
HrvatskoherpetolokodrutvoHyla,Zagreb,Hrvatska(ana.stih2@gmail.com)
NapodrujuHrvatskeobitavajudvijevrsteslatkovodnihkornjaa,rijenakornjaa(Mauremys
rivulata)ibarskakornjaa(Emysorbicularis).Zarazlikuodbarskekornjaekojanaseljavacijelu
dravu,rijenakornjaajeprisutnasamonakrajnjemjuguHrvatske,topredstavljarubnidio
arealaovevrste.UHrvatskojdolazisamoutrimale,geografskiizoliranepopulacijetespadau
najugroenijevrstegmazovauHrvatskoj.Usvrhuprikupljanjaosnovnihpodatakaoekologijii
populacijskoj biologiji rijene kornjae, tijekom 2013. godine zapoeta su istraivanja na
populaciji rijene kornjae u Konavoskome polju. Istraivanja su se provodila na poligonu
veliine2000x500mmetodomulovi/oznai/ponovnoulovi.Poprviputajeocjenjenaveliina,
gustoatedobnaispolnastrukturakonavoskepopulacije.Nanekolikodijelovakanalakojeova
vrsta naseljava, opaeni su u potpunosti betonirani i kanalizirani dijelovi vodotoka, to
predstavljaznaajnuprijetnjurijenojkornjai,aliidrugimvrstamavodozemacaigmazovakoji
kanalekoristekaoprivremenoilistalnostanite.Prikupljenipodatciopopulacijskojbiologijii
ekologijiznaajnoepridonijetipoznavanjurijenekornjaeuHrvatskojtedugoronoosigurati
njenopstanakizatitu.
Kljunerijei:Konavle,populacijskaistraivanja,slatkovodnakornjaa
CHARACTERISTICSOFTHEBALKANTERRAPINPOPULATIONFROMKONAVLEFIELD
A.tih,I.Buri
CroatianHerpetologicalSocietyHyla,Zagreb,Croatia(ana.stih2@gmail.com)
TheterritoryofCroatiaisinhabitedbytwospeciesoffreshwaterturtles:theBalkanTerrapin
(Mauremys rivulata) and the European Pond Turtle (Emys orbicularis). Unlike the European
PondTurtle,whichiswidespreadthroughoutthewholecountry,theBalkanTerrapinispresent
only in the far south which also represents the southwestern edge of the species range. In
Croatia it occurs in three, small geographically isolated populations and is one of the most
endangeredreptilespeciesinthecountry.During2013westartedthepopulationstudieson
the Balkan Terrapin in the area of Konavle field in order to collect basic information about
ecologyandpopulationbiologyoftheBalkanTerrapin.Studieswerecarriedoutona2000by
500 m polygon field using capture/mark/recapture method. Population size and density, age
and sex structure of the population from the Konavle field was estimated for the first time.
Somepartsofthewaterchannel,usedbytheBalkanTerrapin,wererecentlyconcreted,and
thus represents significant threat not just to the Balkan Terrapin but also to all other
amphibianandreptilespecieswhichareusingchannelsaspermanentortemporaryhabitats.
Datacollectedonpopulationbiologyandecologywillsignificantlycontributetotheknowledge
oftheBalkanTerrapininCroatiaandwillensureitslongtermexistenceandprotection.
Keywords:Konavle,populationstudies,freshwaterturtle
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Biologijamora
MarineBiology
O74
TRENUTNISTATUSBIORAZNOLIKOSTIIHTIOFAUNEJADRANSKOGAISREDOZEMNOGAMORA
J.Duli,B.Dragievi
Institutzaoceanografijuiribarstvo,Split,Croatia(dulcic@izor.hr)
Kljunerijei:Sredozemnomore,Jadranskomore,promjene,ribe,bioraznolikost
THECURRENTSTATUSOFTHEADRIATICANDMEDITERRANEANFISHBIODIVERSITY
J.Duli,B.Dragievi
InstituteforOceanographyandFisheries,Split,Croatia(dulcic@izor.hr)
ThemarinebiodiversityoftheMediterraneanSeaisnowadaysfacingtosubstantialstructural
changesinfloraandfauna.SuchchangeswererecordedintheAdriaticSea,aswell.Duringthe
last few decades, various factors including climate change, anthropogenic activity and
lessepsianmigrationhavealteredthecompositionofAdriaticichthyofauna.Thesechangesare
reflectedinthenumberofspeciesquotedinthechecklistofAdriaticfishes,from407in1996
to 440 in 2010. Since 2010, 12 new species were recorded, which raised the number to 452
species and 139 families. Of the 14 Lessepsian migrants that were recorded in the Adriatic,
Fistularia commersonii and Siganus luridus proved to be successful invaders for its southern
part.SeveralchecklistsfortheMediterraneanSeainthelast14yearsquotedthenumberof
Mediterraneanfishspeciesfrom648to664species.Untilto2014,151ofnonindigenousfish
specieswereobservedintheMediterraneanSea(34inthelast5years,atleast36speciesonly
fromtheRedSeasince2000).AsignificantincreaseofLessepsianmigrantsispartlyassociated
with the expansion and deepening of the Suez Canal. Some of them established populations
very quickly in the Mediterranean and some have positive economic significance and some
speciesexhibitnegativeimpactsonmarinefisheriesandbiodiversity.
Keywords:MediterraneanSea,AdriaticSea,changes,fish,biodiversity
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O75
MIKROZOOPLANKTONBOKOKOTORSKOGZALJEVAIOBALNOGPODRUJAJUNOGJADRANA
P.Lui1,J.Njire1,P.Krui2,B.Pestori3,D.Drakulovi3
1
Institut za more i priobalje, Sveuilite u Dubrovniku, Dubrovnik, Hrvatska
(petra.lucic@gmail.com)
2
Zoologijskizavod,Prirodoslovnomatematikifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
3
Institutzabiologijumora,UniverzitetCrneGore,Kotor,CrnaGora
Mikrozooplanktonjevanakomponenta"mikrobnogkruga"iukupnehranidbenemreemora.
Sastav, horizontalna i vertikalna raspodjela brojnosti mikrozooplanktona istraivani su
Niskinovim crpcem (5 L) tijekom proljea 2013. godine na tri postaje unutar Bokokotorskog
zaljeva (Crna Gora) te na postaji Molunat (Hrvatska). Nelorikatni trepetljikai su bili
dominantna skupina mikrozooplanktona (37%) s najviim vrijednostima u povrinskom sloju
gdjejeutjecajslatkevodebiojaeizraen.Ukupnabrojnosttintinidabilajeniskaislinaonim
zabiljeenimnapostajamaotvorenogJadrana.Velikeraznolikostivrstatintinidanaenesuna
svimpostajamasnajveimindeksomraznolikostiuTivatskomzaljevuzbogjakogutjecajaslatke
vodeupovrinskomslojutevodaotvorenogmoraupridnenomsloju.Ukupnojezabiljeeno46
vrsta tintinida od kojih je 20 estuarijskoobalnih te 26 vrsta otvorenog mora. U odnosu na
malobrojnaprethodnaistraivanja23vrstetintinidapoprviputsuzabiljeenezaBokokotorski
zaljev. Vrsta Stenosemella nivalis je dominirala na svim istraivanim postajama. Naupliji
veslonoacabilisubrojnaskupinamikrozooplanktonasudjelomod32%,aznatanudioimalisu
zajedno kopepoditi i mali odrasli veslonoci (17%). Meutim, brojnosti populacija nauplija u
Bokokotorskomzaljevusubilemanjeodglavninepoznatihzazaljeveistoneobaleuproljee.
Ovirezultatimoguseposljedinopovezatisazabiljeenimklimatskimpromjenamautvrenim
za Jadran od 1990.tih godina gdje ve u zimskom razdoblju dolazi do poveane primarne
produkcije,aizostajeproljetnimaksimum.
Kljunerijei:nelorikatnitrepetljikai,tintinidi,naupliji,kopepoditi,juniJadran
MICROZOOPLANKTON IN THE BOKA KOTORSKA BAY AND IN THE COASTAL REGION OF THE
SOUTHADRIATICSEA
P.Lui1,J.Njire1,P.Krui2,B.Pestori3,D.Drakulovi3
1
Institute for Marine and Coastal Research, University of Dubrovnik, Dubrovnik, Croatia
(petra.lucic@gmail.com)
2
DepartmentofBiology,FacultyofScience,UniversityofZagreb,Zagreb,Croatia
3
InstituteofMarineBiology,UniversityofMontenegro,Kotor,Montenegro
Microzooplanktonisimportantcomponentof"microbialloop"andtotalmarinefoodweb.The
horizontal and vertical distributions of microzooplankton were studied during spring 2013 by
Niskin 5L bottle at three stations in Boka Kotorska Bay (Montenegro) and a single station off
Molunat Bay (Croatia). Nonloricate ciliates were the most numerous microzooplankton (37%),
with the highest values at the surface layer that was strongly influenced by the fresh water.
Tintinnidsabundancewerelow,andsimilartothoserecordedintheopenAdriaticSea.Tintinnids
speciesdiversitywasconsiderablyhigh.ThehighestdiversitywasatthestationintheTivatarm
oftheBokaKotorskaBaysystemduetospecificenvironmentalconditionscausedbythestrong
influenceoffreshwaterinthesurfaceandtheopenseawaterinthebottomlayer.Intotal,46
tintinnidswereidentified,amongwhich20wereestuarineneriticand26werecharacteristicfor
theopensea.Comparedtofewpreviousresearches,23specieswererecordedforthefirsttime
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in Boka Kotorska Bay. Stenosemella nivalis was the dominant tintinnid at all stations. Copepod
naupliiwerenumerousandcontributedwith32%intotalmicrozooplanktonabundance,followed
by copepodites and small adult copepods (17%). However, nauplii densities were lower than
those known from the majority of coastal Adriatic region. These results can be linked with the
observed climate changes identified in the Adriatic Sea from the 1990s, where already in the
winterperiodoccurredincreaseofprimaryproductionwiththelackofaspringpeak.
Keywords:nonloricateciliates,tintinnids,nauplii,copepodites,SouthernAdriatic
O76
TAKSONOMIJA I PROSTORNA RASPODJELA FITOPLANKTONA U JUNOM JADRANU; ZIMSKI
ASPEKT
S.Bosak1,M.Mejdandi1,I.Bonjak1,J.Godrijan2,Z.Ljubei1
1
Botaniki zavod/Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska(suncica.bosak@biol.pmf.hr)
2
Centarzaistraivanjemora,InstitutRuerBokovi,Rovinj,Hrvatska
Usvrhuistraivanjaspecifinihobrazacazimsketermohalinecirkulacijeiraspodjeleplanktona
u junom Jadranu u sklopu HRZZ projekta BIOTA, provedeno je oceanografsko istraivanje u
razdobljuod28.veljaedo3.oujka2015.Istraivanjejeobuhvatilo15postajasmjetenihdu
dvatransekta:(i)odDubrovnikadoizobateod1000m(usmjeru210)(P100P1000),te(ii)
od izobate od 1000 m do otoka Lastova u Junojadranskoj kotlini (M100 M1000). Pomou
inverznog svjetlosnog mikroskopa ukupno je analizirano 117 uzoraka i odreeni su sastav i
brojnostfitoplanktonskihvrsta.Maksimalnabrojnostmikrofitoplanktona(1,97x104stanica/L)
te nanofitoplanktona (4.40 x 104 stanica/L) zabiljeena je u povrinskim uzorcima na postaji
P100iP120.Mikrofitoplanktonomsudominiraledijatomeje,anjihovaneobinovelikabrojnost
(<2 x 104 stanica/L) zabiljeena je i u dubokomorskim uzorcima (>300 m dubine).
Taksonomskomanalizomodreenojeukupno146fitoplanktonskihsvojti,odkojihdominiraju
dijatomejesa110morfotipova.DominantnevrstedijatomejabilesuAsterionelopsisglacialis,
Pseudonitzschia sp., Bacteriastrum furcatum te vrste roda Chaetoceros. Nadalje,
preliminarnim analizama odreen je veliki broj neidentificiranih planktonskih penatnih
dijatomeja (34 morfotipa) za iju su precizniju taksonomsku identifikaciju potrebne dodatne
analizetransmisijskimiskenirajuimelektronskimmikroskopom.
Kljunerijei:juniJadran,fitoplankton,dijatomeje
S.Bosak1,M.Mejdandi1,I.Bonjak1,J.Godrijan2,Z.Ljubei1
1
DivisionofBotany/DepartmentofBiology,Zagreb,Croatia(suncica.bosak@biol.pmf.hr)
2
CentreforMarineResearch,RuerBokoviInstitute,Rovinj,Croatia
A total of 117 water samples were analysed and phytoplankton composition and abundance
was determined using an inverted light microscope. Maximal abundance of
microphytoplankton (1.97 x 104 cells/L) and nanophytoplankton (4.40 x 104 cells/L) was
detected in surface samples at P100 and P120 stations, respectively. Diatoms were the
dominant microphytoplankton group, with peculiarly large abundances (< 2 x 104 cells/L)
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detected in deep water samples (> 300 m). Via taxonomic analysis 146 phytoplankton taxa
were determined, out of which diatoms dominated with 110 morphotypes. The dominant
diatoms were Asterionelopsis glacialis, Pseudonitzschia sp., Bacteriastrum furcatum and
species belonging to the genus Chaetoceros. Further, preliminary analyses showed large
number of unidentified planktonic pennate diatoms (34 morphotypes) and for their more
accuratetaxonomicidentificationadditionalanalyseswithtransmissionandscanningelectron
microscopeareessential.
Keywords:southernAdriatic,phytoplankton,diatoms
O77
USKLAENORAZMNOAVANJEALGIRODAUlva
A.uljevi,B.Antoli
Institutzaoceanografijuiribarstvo,Split,Hrvatska(zuljevic@izor.hr)
Zelene alge roda Ulva s preko 130 danas poznatih svojti, jedne su od najuobiajenijih algi
plitkog morskoga dna. O njihovoj biologiji i ekologiji ukljuujui i razmnoavanje, objavljeni
subrojniradovi.Veinaistraivanjarazmnoavanjaobavljenajeulaboratorijskimuvjetima.
Izuzev malog broj usputnih terenskih opaanja, ne postoje radovi o sustavnom istraivanju
fenologije spolnog razmnoavanja koje je provedeno u prirodi.Tijekom proljetnih razdoblja
od2012.do2015.,napodrujuSplitaobavljenasuterenskaistraivanjarazmnoavanjaalgi
rodaUlva.Utvrenojekakoseisputanjegametauklaujesizlaskomsunca.Unutarnekoliko
minuta sve fertilne alge isputaju gamete pri emu se razvija lako uoljiv gametni oblak.
Gametni oblak moe biti toliko intenzivan da smanji prozironost mora gotovo na nulu.
Ovisnoogustoinaseljaalgiiuvjetimaumoru,gametnioblakostajevidljividueodjednog
sata. Sporofiti s druge strane nemaju usklaeno isputanje spora. Zoospore se vjerojatno
isputaju polako tijekom cijeloga jutra. Naglo isputanje zoospora moe biti potaknuto
znaajnim promjenama ekolokih uvjeta kao to je uronjavanje alge nakon isuivanja za
vrijemeoseke.Usklaenoisputanjegametakodsesilnihmorskihorganizamapotrebnojeda
biseosiguralauspjenaoplodnja.Premdajebilaoekivanoikodmorskihsalata,masovnosti
vidljivost dogaaja vie je nego iznenaujua i pokazuje koliko je nae znanje o uobiajnim
vrstamajouvijeknepotpuno.
Kljunerijei:Ulva,alge,razmnoavanje
SYNCHRONIZEDREPRODUCTIONOFUlvaSPECIES
A.uljevi,B.Antoli
InstituteofOceanographyandFisheries,Split,Croatia(zuljevic@izor.hr)
The green algae ofgenus Ulva, withabout 130 accepted taxa, areamongthe most common
algalgroupsofshallowmarinebottom.Therearemanyarticlespublishedontheirbiologyand
ecology,includingreproduction.Themajorityoftheresearchonreproductionwereconducted
inlaboratorybutthereareonlyafewpublicationsonphenologyofreproductioninthefield.
DuringspringtimeintheareaofSplitfrom2012till2015,wehavefoundthatpopulationof
algeUlvareleasedgametesjustwithinthesunrise.Allfertilealgaereleasedgameteswithina
few minutes. That leads to development of the visible green dense gamete cloud can which
reducethevisibilityinthesea.Dependingonthealgaldensityandseastatecondition,gamete
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cloud can persist even over an hour. On the other hand, release of zoospores is not
synchronized. They are probably released slowly during entire morning. Rapid release of
zoosporescanbeprovokedbysignificantchangesinenvironmentalconditions,likeimmersing
afterdesiccationduetolowtide.Synchronizedreleaseofgametesinsessilespeciesisessential
toensuresuccessfulfertilization.Althoughthesynchronizedreleasewasalsopredictedinsea
lettuces,quantityandvisibilityoftheeventsisfarmorethanexpected.Thatalsoindicateshow
ourknowledgeonthecommonspeciesisstillincomplete.
Keywords:Ulva,algae,reproduction
O78
NOVONASTALEPROMJENEFAUNEHIDROMEDUZASJEVERNOGJADRANA
D.Lui1,I.Onofri1,M.Miloslavi1,B.Gangai1,I.Ivani2
1
Institutzamoreipriobalje,SveuiliteuDubrovniku,Dubrovnik,Hrvatska(davor.lucic@unidu.hr)
2
CenterforMarineResearch,RuerBokoviInstitute,Rovinj,Hrvatska
HidromeduzesumeuprvimplanktonskimvrstamaopisanimusjevernomJadranupoetkom20.
stoljea.Ekolokepromjene(eutrofikacija)ismanjenjeotopljenogkisikauzdnood1950.tihpa
nadalje,uspostavilisunoveekolokeuvjeteutomakvatoriju.Tepromjeneuzrokovalesuznatno
smanjenje bioloke raznolikosti bentosa, ukljuivi meroplanktonske meduze. U ovom radu
prikazani su rezultati istraivanja sastava i brojnosti faune hidromeduza sjevernog Jadrana,
imajui u vidu njihovu moguu repopulaciju zadnjih godina. Uzorci planktona su sakupljeni
mjesenovertikalnimpotezimaoddnadopovrinesWP2Nansenovommreomnaetiripostaje.
Predstavljeni rezultati obuhvatila su tri vremenska razdoblja: 1999.2002., 2003.2007. i 2009.
2013. Rezultati jasno ukazuju na porast broja vrsta i njihove brojnosti od prvog razdoblja
istraivanja prema zadnjem (2009.2013.). Pretpostavljamo da su neke od tih vrsta unesene
ulaznimstrujamausjeverniJadran,meutim,njihovabrojnostjeviestrukoveauusporedbis
prijanjim vrijednostima. Zabiljeene visoke prosjene i maksimalne vrijednosti za pojedine
metagenetske vrste ukazujuponovnu mogunostuspostave stabilnihpopulacija.Ovepromjene
suuskladusnovonastalimstanjemsastavaibrojnosticjelokupnogplanktonasjevernogJadrana
uzrokovanihklimatskimpromjenamaiposljedinomoligotrofikacijomistraivanogpodruja.
Kljunerijei:biodiverzitet,planktonskiarnjaci,klimatskepromjene,sjeverniJadran
RECENTCHANGESINTHENORTHERNADRIATICHYDROMEDUSANFAUNA
D.Lui1,I.Onofri1,M.Miloslavi1,B.Gangai1,I.Ivani2
1
University of Dubrovnik, Institute for Marine and Coastal Dubrovnik, Croatia
(davor.lucic@unidu.hr)
2
CenterforMarineResearch,RuerBokoviInstitute,Rovinj,Croatia
HydromedusaewereamongthefirstdescribedplanktonspeciesofthenorthernAdriaticatthe
beginning of 20th century. Ecological changes (eutrofication) and low oxygen saturation near
bottom in this area demonstrated new established trend from 1950s to forward. Such
conditions consequently caused a significant reduction of biodiversity of benthic organisms,
includingmeroplanktonichydrozoans.Inthisstudywepresentthecomparativeresultsofthe
composition and abundance of hydromedusan fauna of northern Adriatic, pointing to the
possibilityofspeciesrepopulatingrecentyears.Planktonwassampledmonthlybybottomto
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surface vertical tows with WP2 Nansen mesh plankton net at four stations of the northern
Adriatic. Presented data are results of investigations during three study periods: 19992002,
20032007 and 20092013. Our results clearly showed that the n umber of species and their
abundanceconsiderableincreasedfromthefirsttothethirdinvestigatedperiod.Weassume
that some species were introduced by input currents in the northern Adriatic, but their
abundanceswereseveraltimeshigherthaninpreviousrecords.Ourhighmeanandmaximum
values of this metagenetic species could indicate the possibility of establishing stable
populationsinthearea.Thesechangesareconsistentwiththerecentchangesintheplankton
composition and abundance, probably caused by a climatic forcing and recognized
oligotrophicationoftheentirenorthernAdriatic.
Keywords:biodiversity,planktoniccnidaria,climaticchange,NorthAdriaticSea
O79
VREMENSKA DINAMIKA ZAJEDNICA KALANOIDNIH KOPEPODA U IZOLIRANOM MORSKOM
JEZERU(VELIKOJEZERO,JUNIJADRAN):VEZESKLIMATSKIMPROMJENAMANAVEOJSKALI
M.Miloslavi,D.Lui
Institut za more i priobalje, Sveuilite u Dubrovniku, Dubrovnik, Hrvatska
(marijana.miloslavic@unidu.hr)
Kljunerijei:kalanoidnikopepodi,dugoronadinamika,Jadranskomore,morskojezero
M.Miloslavi,D.Lui
Institute for Marine and Coastal Research, University of Dubrovnik, Dubrovnik, Croatia
(marijana.miloslavic@unidu.hr)
Wecompliedandanalysedalongtermcalanoidcommunitydataset(19522009)withrespect
toenvironmentalvariationsinnearlyclosedseawaterlakeVelikojezero(SouthAdriaticSea).
Changesinhydrographicpropertieswereinfluencedbydeepeningandbroadeningthechannel
between Veliko Jezero and the sea in the early 1960s, which was in particular reflected by
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increasing salinity. Calanoid abundance displayed high intra and interannual fluctuations.
Calanoids in the lake comprised 22 coastal and estuarine species, with five of them present
overtheentirestudyperiod(Calanushelgolandicus,Paracalanusparvus,Centropageskroyeri,
Isias clavipes, Acartia (Acartiura) clausi). Observed changes low densities in early 1980s; a
shift in the dominant species; reduction and, finally, disappearance of Pseudocalanus
elongatus; increases in Paracalanus parvus and Diaixis pygmaea can be associated with
synchronous changes across trophic levels that have occurred in other European seas. The
results presented here underline the importance of longterm studies of semiclosed marine
lakesandlagoons,astheseareparticularlysensitivetoglobalclimaticchanges.
Keywords:calanoidcopepods,longtermdynamics,Adriaticsea,seawaterlake
O80
UTJECAJ STRATEGIJE HRANJENJA NA DRUTVENU STRUKTURU DOBRIH DUPINA (Tursiops
truncatus)UISTONOMJADRANU,HRVATSKA
G.Plesli1,G.Kett2,N.RakoGospi1,T.Vuur1,D.Holcer1,J.MioiStoi1,P.Mackelworth1
1
Plavi svijet Institut za istraivanje i zatitu mora, Veli Loinj, Hrvatska (grgur.pleslic@plavi
svijet.org)
2
UniversityCollegeofCork,Cork,Irska
Ranije studije opisuju utjecaj koarenja ili marikulture na veliinu i sastav skupina dobrog
dupina (Tursiops truncatus), no do sada nije promatran kombinirani utjecaj. Tijekom 2013. i
2014. provedeno je istraivanje u tri podruja Jadrana: creskoloinjski arhipelag, sjeverna
Dalmacija (Zadarska i ibenskokninska upanija) te viki arhipelag. Cilj istraivanja bio je
utvrditikombiniraniutjecajkoarenjaimarikulturenaveliinu,drutveniidobnisastavskupina
utripodrujakojaserazlikujuuintenzitetukoarenjaimarikulture.Ukupnojezabiljeeno176
opaanjagrupakojesehranenaotvorenommoru(OM),59grupaprilovuizakoarice(K)te8
grupa pri lovu na ribljim farmama (RF). Prosjena veliina skupina bila je (50,56), najvia u
creskoloinjskom (5,810,43), najnia u vikom arhipelagu (3,6 0.73), no nije se znatno
razlikovala meu OM, RF i K skupinama (K=0,138, df=2, p>0,05). Ukupan stupanj asocijacije
jedinki bio je nizak (0,10,1), no vii za RF (0,07 0,03) i K (0,070,03), nego OM skupine
(0,020,01). Hijerarhijska cluster analiza pokazala je visoko strukturiranu ukupnu populaciju
(CCC=0,91), a prisutnost koarica i ribljih farmi ima vei utjecaj na sastav skupina u sjevernoj
Dalmaciji i vikom arhipelagu nego creskoloinjskom arhipelagu. Analiza dobnog sastava
skupinapokazujeveuzastupljenostodraslihjedinkiuTiRF,negouOMskupinama(2=9,21,
p<0,001).Ovirezultatipruajutemeljzadaljnjaistraivanjaiosmiljavanjemjerazatite.
Kljunerijei:dobridupin,Tursiopstruncatus,socijalnaekologija,ribarstvo,zatita
G.Plesli1,G.Kett2,N.RakoGospi1,T.Vuur1,D.Holcer1,J.MioiStoi1,P.Mackelworth1
1
BlueWorldInstituteofMarineResearchandConservation,VeliLoinj,Croatia(grgur.pleslic@plavi
svijet.org)
2
UniversityCollegeofCork,Cork,Ireland
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Previous studies have shown that either bottom trawlers or fish farms influence bottlenose
dolphin(Tursiopstruncatus)groupsizeandstructure,howevernostudydescribedcombined
effect.In2013and2014aphotoIDbasedstudywasconductedinthreeregionsoftheAdriatic
sea:CresLoinj,NorthDalmatiaandVis.Theaimwastoexaminecombinedeffectofbottom
trawlers and fish farms upon group size, social and ageclass structure of bottlenose dolphin
communities in the three regions that differ in intensity of bottom trawler and aquaculture
fisheries.Researcheffortresultedinsightingsof176groupsengagedin"openwaterforaging"
(OWF), 59 in "bottomtrawler foraging" (BTF) and 8 in "foraging at fish farm" (FFF). Overall
group size was (50.56), being highest in CresLoinj (5.810.43) and lowest in Vis region
(3.60.73), but it did not vary significantly between the foraging strategies (K=0.138, df=2,
p>0.05). Simpleratio association index was overall low (0.10.1), but higher for FFF (0.07
0.03)andBTF(0.040.02)thanOWFgroups(0.020.01).Hierarchialclusteranalysesshowed
overallhighlystructuredpopulation(CCC=0.91),structurebeingmoreaffectedbypresenceof
bottom trawlers and fish farms in North Dalmatia and Vis than in CresLoinj region. Age
compositionofgroupsvaried,withmoreadultsinBTFandFFFthaninOWFgroups(2=9.21,
p<0.001). These results provide baseline information for further research and conservation
plans.
Keywords:bottlenosedolphin,Tursiopstruncatus,socialecology,fisheries,conservation
O81
PRILOG POZNAVANJU EKOLOGIJE SPUVE Suberites domuncula (Olivi, 1872): PREKO
MORFOLOGIJEDOANALIZEPOPULACIJE
A.Jaklin1,D.PaviiHamer1,A.Barievi1,W.E.G.Muller2,R.Batel1,B.Hamer1
1
Centarzaistraivanjemora,InstitutRuerBokovi,Rovinj,Hrvatska(jaklin@cim.irb.hr)
2
InstituteforPhysiologicalChemistry,JohannesGutenbergUniversity,Mainz,Germany
Kljunerijei:Suberitesdomuncula,Jadran,pigmenti,citokromoksidaza,barkodiranje
CONTRIBUTIONTOTHEECOLOGYOFTHESPONGESuberitesdomuncula(Olivi,1872):FROM
MORPHOLOGYTOPOPULATIONANALYSES
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A.Jaklin1,D.PaviiHamer1,A.Barievi1,W.E.G.Muller2,R.Batel1,B.Hamer1
1
CenterforMarineResearch,RuerBokoviInstitute,Rovinj,Croatia(jaklin@cim.irb.hr)
2
InstituteforPhysiologicalChemistry,JohannesGutenbergUniversity,Mainz,Germany
Keywords:Suberitesdomuncula,Adriatic,pigments,cytochromeoxydase,barcoding
O82
EKOLOKI STATUS LIVADA MORSKE CVJETNICE Posidonia oceanica UZDU HRVATSKE
OBALE
I.Cvitkovi,M.Despalatovi,A.uljevi,S.Roi,B.Antoli
Laboratorijzabentos,Institutzaoceanografijuiribarstvo,Split,Hrvatska(cvite@izor.hr)
Morska cvjetnica Posidonia oceanica i njene livade su od 2012. godine uvrtene u program
praenjazapotrebeOkvirnedirektiveovodama.Dabiseodredioekolokistatuslivada,koristise
modificiranimultivarijatniindekszaPosidoniaoceanica(POMI9).Ovdjedonosimoekolokistatus
za45livadakojesenalazeuzduitavehrvatskeobale.Trinaestlivadaobraenojetijekom2014.
godine,14livadatijekom2012.i2013.,te18livadatijekomimplementacijskefazeDirektive2007.
godine. Za 39 livada je zabiljeen dobar i vrlo dobar ekoloki status, za 3 livade je zabiljeen
umjereniekolokistatus,zadvijelivadelo,tezajednulivaduvrloloekolokistatus.Livadeza
koje nije zabiljeen dobar i vrlo dobar status se nalaze u neposrednoj blizini luka, uzgajalita i
sidrita. Analize potvruju da su livade morske cvjetnice P. oceanica uzdu hrvatske obale u
zadovoljavajuem(dobromivrlodobrom)ekolokomstatusu.
Kljunerijei:ekolokistatus,Posidoniaoceanica
ECOLOGICALSTATUSOFPosidoniaoceanicaMEADOWSALONGTHECROATIANCOAST
I. Cvitkovi,M.Despalatovi,A.uljevi,S.Roi,B.Antoli
Laboratoryforbenthos,InstituteofOceanographyandFisheries,Split,Croatia(cvite@izor.hr)
SeagrassPosidoniaoceanicaanditsmeadowsinCroatiancoastalareaoftheAdriaticSeahave
beenmonitoringforthepurposeofWaterFrameDirectivesince2012.Toassesstheecological
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status of P. oceanica meadows, modified Posidonia oceanica multivariate index (POMI9) has
beenused.Herewepresenttheecologicalstatusof45Posidoniameadowslocatedalongthe
Croatiancoast.Thirteenmeadowswereexaminedduringthemonitoringprogramin2014,14
meadows were examined in the period 2012 2013 and 18 meadows during the
implementation phase of the Directive in 2007. As many as 39 meadows had good and very
goodecologicalstatus,threehadmoderateecologicalstatus,twohadbadandonehadvery
bad ecological status. Meadows with very bad, bad and moderate ecological status were in
vicinity of ports, mariculture facilities or in anchoring sites. According to analyses meadows
alongtheCroatiancoastareinsatisfying(goodandverygood)ecologicalstatus.
Keywords:ecologicalstatus,Posidoniaoceanica
O83
RANI RAZVOJNI STADIJI DVA TROPSKA PUA (OPISTHOBRANCHIA) U KLIMATSKIM
PROMJENAMA
M.Bilan1,3,G.Dionsio1,2,R.Calado2,R.Rosa1
1
MAREMarineandEnvironmentalSciencesCentre,LaboratrioMartimodaGuia,Centrode
Oceanografia,FaculdadedeCinciasdaUniversidadedeLisboa,Cascais,Portugal
2
Departamento de Biologia & CESAM, Universidade de Aveiro, Campus Universitrio de
Santiago,Portugal
3
Sveuilinistudijzastudijemora,SveuiliteuSplitu,Split,Hrvatska(meribilan@gmail.com)
Kljunerijei:Opisthobranchia,klimatskepromjene,ranirazvojnistadiji
ONTOGENICDEVELOPMENTOFTWOTROPICALOPISTHOBRANCHMOLLUSKSINACHANGINGOCEAN
M.Bilan1,3,G.Dionsio1,2,R.Calado2,R.Rosa1
1
MAREMarineandEnvironmentalSciencesCentre,LaboratrioMartimodaGuia,Centrode
Oceanografia,FaculdadedeCinciasdaUniversidadedeLisboa,Cascais,Portugal
2
Departamento de Biologia & CESAM, Universidade de Aveiro, Campus Universitrio de
Santiago,Aveiro,Portugal
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12.HBK9012 CBC
This study investigated the interactive effects of warming and CO2driven acidification on
fertilization, development and juvenile survival of two tropical Opisthobranchs, Berghia
stephanieaeandElysiaclarki.Thesemarinetropicalseaslugsarecommonlyusedasbiological
tools for scientific research particularly to study their chemical ecology and photosymbiotic
associations.Fourexperimentaltreatments(varyingfrom26Cto30CandpHfrom8to7.5)
weretestedinallcombinationsandcomparedwiththecontrolscenario(26C,pH8).E.clarki
and B. stephanieae marine sea slugs were severely impacted by the effects of warming and
acidification. Contrary to global warming, ocean acidification reduced the number of egg
masses per individual and development in both species. For B. stephanieae the combined
effectsofwarmingandacidificationweresooutstandingthatnoeggmassesevolvedafterthe
6 day of deposition. Also, severe veliger deformities (body, shell and oral apparatus) were
observed for both species at ocean warming and acidification treatment (93 and 60%
deformedveligersinBerghiaand Elysia, respectively).Concomitantly,forthesesea slugsthe
futurescenario isnot promising, since juveniles survivalwas negatively impacted.Our study
emphasizes the need for experiments that address ocean warming and acidification on
Opisthobranchsconcurrentlyandplacesinglestressorstudiesincontext.
Keywords:Opisthobranchia,climatechange,earlystages
O84
MORFOLOGIJAISTRUKTURAKUICEPUEVASTRANJOKRNJAKAAplysiaspp.
A.Jaklin1,F.Marin2,D.Medakovi1,2
1
CentarzaistraivanjemoraRovinj,InstitutRuerBokovi,Rovinj,Hrvatska(jaklin@cim.irb.hr)
2
UMRCNRS6282Biogosciences,UniversitdeBorgougne,Dijon,Francuska
Kljunerijei:Aplysiafasciata,Aplysiapunctata,veliger,unutarnjakuica,biominerali
MORPHOLOGYANDSTRUCTUREOFTHEOPISTHOBRANCHGASTROPODSHELLAplysiaspp.
A.Jaklin1,F.Marin2,D.Medakovi1,2
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Center for Marine Research Rovinj, Ruer Bokovi Institute, Rovinj, Croatia
(jaklin@cim.irb.hr)
2
UMRCNRS6282Biogosciences,UniversitdeBorgougne,Dijon,France
Related sea slug species Aplysia punctata and A. fasciata (Gastropoda, Opisthobranchia,
Anaspidea) occur simultaneously in theMediterraneanand Adriatic infralittoral coastal zone.
During larval growth and development spiral shell completely encloses and protects the
specimen's soft body. After veliger metamorphosis, the external shell becomes internal and
reduced to a simply concave shield over soft viscera. Scanning electron microscopy (SEM),
Fourier transform infrared spectroscopy (FTIR) and Xray diffraction (XRD) were used to
determinethemorphology,physical,structuralandcrystallographiccharacteristicsoftheadult
shells of both species. Concomitantly, a comparative study via different molecular
characterizationswascarriedoutbymeasuringenzymaticactivitiesofcarbonicanhydrase(CA),
andbyperformingproteomicsandimmunohistologicallocalizationbyasetofantibodies.Adult
A.punctatashellsconsistoftwoorganiclayersbetweenwhichisinsertedacalcifiedlayerwith
dominantaragonite(71M%)andfeldspar(19M %)crystals.Theadultinternalshellsofthe
relatedspeciesA.fasciatawerefoundtoconsistalmostonlyofaragonitecrystals(about90M
%).Thehalitecrystalsarepresentontheinternalandoutsidesurfacesoftheshell.Theyare
characteristic of both species, but differ in shape, orientation and direction of the crystal
growth.
Keywords:Aplysiafasciata,Aplysiapunctata,veliger,internalshell,biominerals
O85
IVOTNI CIKLUS PSA MEKUA (Mustelus punctulatus) U SJEVERNOM I SREDNJEM
JADRANSKOMMORU
R.Graan1,T.Polak2,G.Lackovi1,B.Lazar3,4
1
Zoologijski zavod, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska(romana.gracan@biol.pmf.hr)
2
Biolokiodsjek,Prirodoslovnomatematikifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
3
Department of Biodiversity, Faculty of Mathematics, Natural Sciences and Information
Technologies,UniversityofPrimorska,Koper,Slovenia
4
StudijZnanostomoru,SveuiliteJurjaDobrileuPuli,Pula,Hrvatska
Pas meku (Mustelus punctulatus Risso, 1827) je uestala vrsta pridnenog morskog psa koja se
komercijalnoizlovljava,apremaIUCNovomCrvenompopisusvrstanajeukategorijunedovoljno
poznate svojte. Uporabom histolokih i morfometrijskih metoda na spolnim organima i
kraljecima istraili smo demografske i reproduktivne karakteristike psa mekua. Uzorci su
prikupljeni od 220 jedinki (108 enki i 112 mujaka) ulovljenih pridnenim koama od 2005. do
2007.godineusjevernomisrednjemJadranu.Ulovjebioravnomjernorasporeenposezonama,
osim tijekom ljeta kada je prikupljeno samo 7 ivotinja.enke suu prosjeku imale vee duine
tijela (DT) (srednja vrijednost DT: 77,1 22,1 cm) od mujaka (76,9 20,4 cm), i bile starije
(srednjavrijednost:7,24,6godina)odmujaka(6,74,0godina).Najstarijaprocijenjenadobza
enkeiznosilaje22godine,dokjedobnajstarijegmujakaprocijenjenana16godina.Uuzorkusu
prevladavalejedinke(74%)starostiizmeu2+i9+godina.Procijenjenastarostnajmanjetrudne
enke (DT: 114,9 cm) bila je 12 godina, dok je najmanji mujak (DT: 74,3 cm) s razvijenim
sjemenicimabiostar7godina.Dobivenirezultatiukazujudajepasmekukselektivnavrsta,koja
imasporisomatskirast,kasnospolnosazrijevanjeidugiivotniciklusimejeposebiceosjetljiva
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naizlov.StogajezaouvanjepopulacijeuJadranskommorukljunoodrivoupravljanjeiizrada
akcijskogplanazazatituovevrste.
Kljunerijei:brzinasomatskograsta,spolnazrelost,morskipas,kselektivnavrsta
LIFEHISTORYTRAITSOFABLACKSPOTTEDSMOOTHHOUND(Musteluspunctulatus)INTHE
NORTHERNANDCENTRALADRIATICSEA
R.Graan1,T.Polak2,G.Lackovi1,B.Lazar3,4
1
DivisionofZoology,Departmentof Biology, FacultyofScience,UniversityofZagreb,Zagreb,
Croatia(romana.gracan@biol.pmf.hr)
2
Student,DepartmentofBiology,FacultyofScience,UniversityofZagreb,Zagreb,Croatia
3
Department of Biodiversity, Faculty of Mathematics, Natural Sciences and Information
Technologies,UniversityofPrimorska,Koper,Slovenia
4
MarineSciencesProgram,JurajDobrilaUniversityofPula,Pula,Croatia
Keywords:somaticgrowthrate,sexualmaturity,sharks,kselectedspecies
O86
NASELJA KORALJA NA KORALIGENU PUINSKIH PLIINA U ISTONOM DIJELU JADRANSKOG
MORA
P.Krui1,M.Kvarantan2,S.akor2,V.Loni3,S.Motah4,M.Andri5
1
Zoologijskizavod,Prirodoslovnomatematikifakultet,Zagreb,Hrvatska(pkruzic@zg.biol.pmf.hr)
2
PoliklinikaKvarantan,Zagreb,Hrvatska
3
RonilakicentarZlatnaLuka,Bibinje,Hrvatska
4
MASA,Ljubljana,Slovenija
5
CarHercd.o.o.,Zagreb,Hrvatska
Uovomistraivanjuopisujesekoraligenpuinskihpliinanadubinamaod25do100m,naetiri
lokaliteta pokraj Dugog otoka i otoka Kornat (istoni dio Jadrana). Istraivanja su napravljena
metodom ronjenja i snimanja ROV ronilicom. Pregledana su naselja gorgonija Paramuricea
clavataiEunicellacavolini,zajednosanetorjeomvrstomSavaliasavaglia.Naseljaovihvrsta
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12.HBK9312 CBC
rasprostranjenasunadvarazliitastanitapliine:vieizdignutastijenaslinatornju("sika")na
kojojprevladavautagorgonijaipliinasalaganimpadom(mijeanjestijeneiljuturnogpijeska),
gdjeprevladavajuostaladvakoralja.Istraivanepostajesupodvelikimutjecajemribarenja,koje
uzrokujeoteenjanamnogimkolonijama.Oteenjanagorgonijamakasnijeprerastajuepibionti
(poput crvenih alga i obrubnjaka). Ove puinske pliine popularne su malim i profesionalnim
ribarima zbog bogate faune riba. Velik problem puinskog koraligena su premala istraenost i
voljazautvrivanjemutjecajaribarstvanaovebentoskezajednice.Upravljanjetimpodrujimaje
takoer problem, jer nedostaje uinkovitih rjeenja. Ovo izvjee predstavlja preliminarne
podatkeistraivanjaidoprinosipoznavanjudubokogkoraligenaistonogdijelaJadranskogmora.
Kljunerijei:koraligen,koralji,Jadranskomore
P.Krui1,M.Kvarantan2,S.akor2,V.Loni3,S.Motah4,M.Andri5
1
Department of Biology, Faculty of Science, Zagreb University, Zagreb, Croatia
(pkruzic@zg.biol.pmf.hr)
2
PolyclinicKvarantan,Zagreb,Croatia
3
DivingcenterZlatnaLuka,Bibinje,Croatia
4
MASA,Ljubljana,Slovenia
5
CarHercLtd,Zagreb,Croatia
Inthisstudywecharacterizedcoralassemblagesofanoffshorecoralligenebanksdwellingat
50100mdepthonafourlocationsnearIslandsofDugiotokandKornati(easternAdriaticSea).
Surveys were made by scuba diving and ROVimaging. Assemblages of suspension feeders,
dominatedbythegorgoniansParamuriceaclavataandEunicellacavolinitogetherwithSavalia
savaglia, were examined. This three surveyed species were distributed into two areas
corresponding to different habitat preferences: a more elevated hard ground ("steeple")
hosting yellow gorgonian and a gently sloping, silted rocky bottom ("pyramid") hosting the
othertwocoralspecies.Thestudyareaissubjectedtoaheavypressurefromtheprofessional
fishery, resulting in the mechanical damage of numerous colonies, some of which are then
overgrown by various epibionts (mostly red algae and hydrozoans). These offshore rocky
banksarewidelyknownamongrecreationalandprofessionalfishermenduetotheirrichfish
fauna. The main problem about offshore coralligene bank is the lack of knowledge and also
effort to characterizing the extent of the impact and consequences on these benthic
communities.Themanagementoftheseareasisbigissue,stilllackingofaneffectivesolution
in the Adriatic Sea. This report represents a preliminary data of the whole study and
contributestotheknowledgeofdeepcoralligenebanksintheeasternAdriaticSea.
Keywords:Coralligenous,Corals,AdriaticSea
O87
PROSTORNARASPODJELABIOOPTIKIHITERMOHALINIHSVOJSTAVAUSJEVEROZAPADNOM
DIJELUJUNOJADRANSKEKOTLINE
I.Bonjak1,H.Mihanovi2,I.Cetini3,4,P.Krui1,S.Bosak1,M.Mejdandi1,Z.Ljubei1
1
Sveuilite u Zagrebu, Prirodoslovnomatematiki fakultet, Botaniki zavod, Zagreb, Hrvatska
(ivana.bosnjak@biol.pmf.hr)
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12.HBK9412 CBC
Institutzaoceanografijuiribarstvo,Laboratorijzafizikumora,Split,Hrvatska
IraC.DarlingMarineCenter,SveuiliteuMaineu,USA
4
NASAGoddardSpaceFlightSpaceCenter,Columbia,Maryland,USA
Kljune rijei: juni Jadran, zimska dinamika vodenih masa, distribucija fitoplanktona, bio
optikamjerenja
I.Bonjak1,H.Mihanovi2,I.Cetini3,4,P.Krui1,S.Bosak1,M.Mejdandi1,Z.Ljubei1
1
University of Zagreb, Faculty of Science, Department of Botany, Zagreb, Croatia
(ivana.bosnjak@biol.pmf.hr)
2
InstituteofOceanographyandFisheries,PhysicalOceanographyLaboratory,Split,Croatia
3
IraC.DarlingMarineCenter,UniversityofMaine,USA
4
NASAGoddardSpaceFlightSpaceCenter,Columbia,Maryland,USA
BiogeochemicalprocessesintheAdriaticSeaarehighlyinfluencedbycirculationpatternsand
water mass dynamics. In order to investigate specific winter circulation and distribution of
phytoplankton,anoceanographiccruisewasconductedfromFebruary28thtillMarch3rd2015
in the southern Adriatic. Two major transects were investigated: (i) Dubrovnik to 1000 m
isobath (in direction 210), and (ii) 1000 m isobath to Lastovo island. The surveyed area is
greatlyinfluencedbytheLevantineIntermediateWater(LIW)andEastAdriaticCurrent(EAC).
ThewarmandsalineLIWrepresentsapartoftheEACandenterstheAdriaticfromtheIonian
Seaspreadingnorthwardsattheintermediatelayer,usuallyatdepthsbetween100and600m,
with the core from 200 to 400 m. Total of 45 CTD casts were performed and concurrent
measurementsoftemperature,salinityandbioopticalparameters;bioopticalmeasurements
of chlorophyll fluorescence (Chl F), optical particulate backscattering and beam attenuation
were collected. The surveyed area demonstrated circulation dependent high patchiness in
distribution of Chl F. The significant Chl F signal was detected up to 450 m depth, indicating
eitheractiveprimaryproductionindeeplayersorrecentsubductionofthesurfacewater.The
core of the LIW was not as pronounced as in previous years. However, higher salinity and
temperaturevalues,togetherwithdissolvedoxygenminimumwereobservedaround450m.
3
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12.HBK9512 CBC
Keywords:southernAdriatic,winterwatermassdynamics,phytoplanktondistribution,bio
opticalparameters
O88
KORALJIPODMORSKIHPILJAJADRANSKOGMORA
P.Krui
Zoologijski zavod, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Zagreb, Hrvatska
(pkruzic@zg.biol.pmf.hr)
Kljunerijei:koralji,morskepilje,Jadranskomore
CORALFAUNAOFSUBMERGEDMARINECAVESINTHEADRIATICSEA
P.Krui
Department of Biology, Faculty of Science, Zagreb University, Zagreb, Croatia
(pkruzic@zg.biol.pmf.hr)
Marinecavesarewidelyacknowledgedfortheiruniquebiodiversityandconstituteatypical
feature of the Mediterranean coastline. Because of karstic origin, submerged or partially
submergedseacavesarecommonalongeasternAdriaticSea.Themainproblemaboutsea
caves (the biocoenosis of semidark caves) in the Adriatic Sea is that these habitats are
insufficientlystudied.Qualitativesamplingwasperformedinfifteenmarinecavesalongthe
Croatian coast. A total of 22 anthozoan species were recorded in the researched marine
caves. The most common anthozoans were anemones Cerianthus membranaceus
(Spallanzani, 1784), Halcampoides purpureus (Studer, 1879) and Parazoanthus axinellae
(Schmidt,1862),scleractiniancoralsCeratotrochusmagnaghiiCecchini,1914,Leptopsammia
pruvoti LacazeDuthiers, 1897, Hoplangia durotrix Gosse, 1860, Phyllangia americana
mouchezii (LacazeDuthiers, 1897), Caryophyllia (Caryophyllia) inornata (Duncan, 1878),
Polycyathus muellerae (Abel, 1959), Madracis pharensis (Heller, 1868) and Parazoanthus
axinellae (Schmidt, 1862), as well as the endangered gorgonian species Corallium rubrum
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12.HBK9612 CBC
(Linnaeus, 1758). All these coral species could be found on the cave walls, while the
anemoneC.membranaceusisquiteabundantonsandy(detritic)ormuddysubstrateatthe
bottom of the caves. Results indicate that the different topography, depth and geographic
locationofsubmergedcaves,determinecoraldistributionalongthemarinecaves.
Keywords:corals,marinecaves,AdriaticSea
6.SimpozijHrvatskogDrutvazabiljnubiologiju
6thSymposiumoftheCroatianSocietyofPlantBiologists
O89
PROTEIN TaMAB2 SUDJELUJE U REMODELIRANJU KROMATINA I METABOLIZMU RNA U
ZIGOTII2STANINOMPROEMBRIJUPENICE
N.Bauer1,M.Jurani1,M.Moibob1,G.Razdorov2,D.LeljakLevani1
1
Prirodoslovno matematiki fakultet, Sveuilite u Zagrebu, Zagreb, Hrvatska
(natasa.bauer@biol.pmf.hr)
2
Farmaceutskobiokemijskifakultet,SveuiliteuZagrebu,Zagreb
ProteinskaporodicaMATHBTBzajednikajeizaivotinjeibiljke.Zanimljivajeinjenicadagenomi
uronjakaiovjekakodirajusamonekolikoMATHBTBproteina(est,odnosnodva)dokseunekim
biljnimiivotinjskimorganizmimaovaporodicaproteinaproirilanaviedesetakalanova.Izmeu
nekolikodesetakaMATHBTBgenaprisutnihugenomupeniceidentificiralismodva(TaMAB12)
eksprimirana iskljuivo u jajnoj stanici (TaMAB1) ili proembriju (TaMAB2). Gen TaMAB2 kodira
protein koji u interfaznoj stanici lokalizira s mikrotubulima, na asimetrian nain u podruju
jezgrineovojniceiunutarinterfaznejezgre,doktijekommitozeproteinslijedipozicijeformiranja
diobenog vretena i fragmoblasta. tovie, tijekom diobe zigote TaMAB2 uvijek se naslijeuje u
bazalnustanicuproembrija.Asimetrinalokalizacijainasljeivanjeukazujunamoguuukljuenost
proteina TaMAB2 pri uspostavi asimetrine stanine diobe i stanine specifikacije. Analizama
interakcijaproteinasproteinomTaMAB2pokazalismodauspostavljaizravnuinterakcijusculinom
3 (podjedinica ligaze E3) to ukazuje na njegovo sudjelovanje u ubikvitinskom putu razgradnje
proteina.Spektrometrijommaseidentificiralismoveibrojproteinakojiukazujunamogueuloge
TaMAB2primodeliranjukromatinairegulacijimetabolizmaRNA.
Kljunerijei:MATHBTB,Triticumaestivum,staninalokalizacija,asimetrinadioba,TAPtag
N.Bauer1,M.Jurani1,M.Moibob1,G.Razdorov2,D.LeljakLevani1
1
FacultyofScience,UniversityofZagreb,Zagreb,Croatia(natasa.bauer@biol.pmf.hr)
2
FacultyofPharmacyandBiochemistry,UniversityofZagreb,Zagreb,Croatia
TheMATHBTBproteinfamilyiscommontobothanimalsandplants.Itisaphenomenonthat
Arabidopsisandhumangenomesencodeonlyfewmembers(sixandtwo,respectively)ofthe
MATHBTBproteinfamilybutinsomeplantandanimalorganisms,thesameproteinfamilyhas
expanded more than 10fold. Among tens of MATHBTB genes in wheat genome, we have
identified two MATHBTB genes expressed exclusively in egg cell (TaMAB1) or in two celled
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12.HBK9712 CBC
proembryo (TaMAB2). The zygotic induced gene TaMAB2 encodes a protein that
asymmetrically colocalises with microtubuli around the nuclear envelope and within an
interphasenucleus,whileduringmitosisproteinfollowsspindleandphragmoblastformation.
Moreover, zygote deposited TaMAB2 is always inherited to the large basal cell after first
asymmetriczygoticdivision.Theasymmetricallyinheritanceindicatethattheproteinmightbe
involvednotonlyintoestablishmentofasymmetrybutalsointothecellspecificationintwo
celledembryo.ByinteractionstudiesweshowedthatTaMAB2directlyinteractwithCullin3
based E3 ligases and are involved in ubiquitindependent degradation pathway. By mass
spectrometrywehaveidentifiedasetofnovelinteractingproteinsthatindicatethepossible
rolesofTaMAB2inchromatinremodellingandRNAmetabolism.
Keywords:MATHBTB,Triticumaestivum,intracellularlocalization,asymmetricdivision,TAPtag
O90
OPORAVAKKLIJANACAJEMAREHIDRACIJOMNAKONSTRESAIZAZVANOGSUOM
J.AntunoviDuni1,Lj.Mihaljevi1,D.imi2,A.Lali2,J.Kovaevi2,V.Cesar1
1
Odjel za biologiju, Sveuilite Josipa Jurja Strossmayera u Osijeku, Osijek, Hrvatska
(jantunovic@biologija.unios.hr)
2
PoljoprivredniinstitutOsijek,Osijek,Hrvatska
Kljunerijei:Hordeumvulgare,sua,oporavak,relativnisadrajvodeulistu,fotosinteza
RECOVERYOFDROUGHTSTRESSEDBARLEYSEEDLINGSBYREWATERING
J.AntunoviDuni1,Lj.Mihaljevi1,D.imi2,A.Lali2,J.Kovaevi2,V.Cesar1
1
Department of Biology, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
(jantunovic@biologija.unios.hr)
2
AgriculturalInstituteOsijek,Osijek,Croatia
Droughttoleranceisoneofthemoststudiedfeaturesconsideringtheglobalproblemofwater
deficit.Hereweinvestigatedrecoveryofdehydratedandpoorlyviablebarleyseedlingsupon
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12.HBK9812 CBC
rewatering. Barley seedlings (Hordeum vulgare L. cv. Bravo) were cultivated in the growth
chamber(16hday,~65molm2s1,221oC;8hnight,19oC)fortendaysandthensubjected
todroughtinducedbywithholdingwater.After8days,whenleafrelativewatercontent(RWC)
wasabout20%seedlingswerewatereddailywiththesameamountofwaterascontrols.For
allmeasurements,firstfullydevelopedleavesweresampledbeforestress(0thday),8thdayof
droughtandevery24hduringrehydrationperiodoffourdays.Increasedperformanceindex
(PIABS), the parameter of chlorophyll a fluorescence called index vitality, and
immunodetectionofRubiscoLSUrevealedefficientphotosyntheticapparatusafterrewatering
period. Increasing of RWC, decreasing the lipid peroxidation level, and degradation of
accumulatedprolineindicatedhightendencyfortheestablishmentofhomeostasispreviously
disrupted by drought. We can conclude that despite the low RWC and downregulated
photosynthesis under severe drought, seedlings were able to recover their overall
photosyntheticefficiency(PIABS)attheendofrehydrationperiod.Obtainedresultsmightbe
helpfultobarleybreedersduringselectionofcultivarsinordertocreateanewgenerationof
genotypesbetteradaptedtowaterdeficit.
Key words: Hordeum vulgare, drought stress, recovery, leaf relative water content,
photosynthesis
O91
FOTOSINTETSKI ODGOVOR MLADIH I RAZVIJENIH LISTOVA SMOKVE NA VISOKI INTENZITET
SVJETLOSTI
S.Mlinari1,V.Cesar1,H.Lepedu2
1
Odjel za biologiju, Sveuilite J. J. Strossmayera u Osijeku, Osijek, Hrvatska
(selma.mlinaric@biologija.unios.hr)
2
Filozofskifakultet,SveuiliteJ.J.StrossmayerauOsijeku,Osijek,Hrvatska
Visoki intenzitet svjetlosti esto uzrokuje fotoinhibiciju zbog ogranienog transporta elektrona i
smanjenogkvantnogprinosafotosinteze.Ciljjebioistraitiutjecajvisokogintenzitetasvjetlostina
fotosintetsku uinkovitost mladih (YL) i razvijenih (ML) listova smokve (Ficus carica L.) izloenih
povienojtemperaturi.Obatipalistovaaklimatiziranisu12humrakunasobnojtemperaturite
nakontogaizloenitemperaturiod351Cukombinacijisniskim(LI,~50molm2s1)ilivisokim
(HI, ~800 mol m2 s1) intenzitetom svjetlosti tijekom 4 h. Za odreivanje fotosintetske
uinkovitostimjerenajefluorescencijaklorofilaametodomsaturacijskogpulsa.Visokavrijednost
maksimalnog kvantnog prinosa fotosustava II izmjerena na LI tretmanu ukazuje da su oba tipa
listovapotpunofunkcionalna.TretmanHIizazvaojekodMLznaajanpadFv/Fm,smanjenustopu
elektronskog transporta i nisku razinu nefotokemijskog gaenja fluorescencije klorofila a.
Poveanje kvantnog prinosa nereguliranog rasipanja energije (Y(NO)) u kombinaciji s padom
optimalnog kvantnog prinosa (Y(PSII)) i kvantnog prinosa rasipanja energije ovisnom o pH
gradijentu (Y(NPQ)) ukazuju na smanjeni kapacitet zatite ML. YL nisu pokazali razlike izmeu
mjerenih parametara nakon izlaganja LI i HI tretmanima to ukazuje na stabilnu fotosintetsku
uinkovitost. Moe se zakljuiti da su nakon izlaganja HI tretmanu YL zadrali uinkovite
mehanizmezatite,dokjepojaanaosjetljivostMLrezultiralafotoinhibicijom.
Kljunerijei:FicuscaricaL.,fotosinteza,svjetlosnistres,fluorescencijaklorofilaa
PHOTOSYNTHETICRESPONSEOFYOUNGANDMATUREFIGLEAVESTOHIGHIRRADIATION
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12.HBK9912 CBC
S.Mlinari1,V.Cesar1,H.Lepedu2
1
Department of Biology, J. J. Strossmayer University of Osijek, Osijek, Croatia
(selma.mlinaric@biologija.unios.hr)
2
FacultyofHumanitiesandSocialSciences,J.J.StrossmayerUniversityofOsijek,Osijek,Croatia
High irradiation often causes photoinhibition due to limited electron transport and reduced
photosyntheticquantumconversion.Weaimedtoinvestigateinfluenceofhighirradiationon
photosyntheticperformanceofyoung(YL)andmature(ML)figleaves(FicuscaricaL.)exposed
to elevated temperature. Detached YL and ML were acclimated at room temperature in
darknessfor12handthenexposedto351Ccombinedwithlow(LI,~50molm2s1)orhigh
irradiation(HI,~800molm2s1)for4h.Toevaluateprimaryphotochemistryofphotosystem
II(PSII),modulatedchlorophyllafluorescencewasmeasured.Highmaximumquantumyieldof
PSII photochemistry (Fv/Fm) indicated full functionality of both leaf types exposed to LI.
ExposureofMLtoHIinducedsignificantreductionofFv/Fm,followedwithdecreasedelectron
transportrate(ETR)andnonphotochemicalquenching(NPQ).Increaseinthequantumyieldof
nonregulated energy dissipation (Y(NO)), accompanied with decline in both, the effective
quantumyieldofphotochemical energyconversioninPSII(Y(PSII))and thequantumyieldof
pHdependant energy dissipation (Y(NPQ)), suggested reduced capacity of photoprotective
reactions. YL exposed to HI showed no significant difference in all measured parameters
compared to LI, thus indicating stable photosynthetic performance. In conclusion, YL
maintainedefficientphotoprotectivemechanismsatHI,whileincreasedsusceptibilityofMLto
HIresultedwithseverephotoinhibition.
Keywords:FicuscaricaL.,photosynthesis,lightstress,chlorophyllafluorescence
O92
ODREIVANJE IZOFORMI PEROKSIDAZA SKUPINE III I LAKAZA TIJEKOM RAZVOJA STABLJIKE
JEMA(HordeumvulgareL.)
L.Begovi1,D.Pavokovi2,S.Mlinari1,A.Lali3,V.Cesar1
1
Odjel za biologiju, Sveuilite J. J. Strossmayer u Osijeku, Osijek, Hrvatska
(lbegovic@biologija.unios.hr)
2
Zavod za molekularnu biologiju, Bioloki odsjek, Prirodoslovnomatematiki fakultet,
SveuiliteuZagrebu,Zagreb,Hrvatska
3
PoljoprivredniinstitutOsijek,Osijek,Hrvatska
EnzimiperoksidazeskupineIIIsudjelujuubrojnimfiziolokimprocesimaubiljkama.Zajednos
enzimimalakazamaukljuenesuuoksidacijumonolignolakaozavrnogkorakausintezilignina.
Lignin je prirodni polimer koji izgrauje staninu stijenku i daje vrstou stabljici. U ovom
istraivanju cilj je bio utvrditi karakteristike pojedinih izoformi peroksidaza i lakaza u
internodijima tijekom etiri razvojne faze stabljike jema: elongacija, klasanje, cvatnja i
nalijevanje zrna. Izolirane su ukupne peroksidaze (tPOD) i lakaze te peroksidaze kovalentno
vezane za staninu stijenku (cPOD). Izoenzimske forme razdvojene su izolelektrinim
fokusiranjem na poliakrilamidnom gelu. Za utvrivanje peroksidazne aktivnosti koriten je
gvajakol, a za lakaze siringaldazin. Analiza je pokazala prisutnost 8 razliitih izoformi tPOD s
izoelektrinom tokom (pI) 810 i jedne izoforme s pI 2,55. Najvea aktivnost uoena je u
prvom internodiju u svim razvojnim fazama. U staninoj stijenci cPOD prisutne su u jednoj
izoformi,sprofilomaktivnostislinomkaotPOD.Lakazesuzastupljenesdvijeizoforme(pI810
i pI 2,55) bez razlike u jaini aktivnosti u pojedinom internodiju i razvojnoj fazi. Dobiveni
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rezultatiukazujunaprisutnostikarakteristikerazliitihizoformiperoksidazailakazaukljuenih
urazvojneproceseustabljicijema.Buduaistraivanjaimajuzaciljrazjasnitiulogupojedinih
izoformiuprocesubiosintezelignina.
Kljunerijei:internodij,staninastijenka,lignin,izoforme,bakarvezaneoksidaze
DETERMINATIONOFCLASSIIIPEROXIDASEANDLACCASEISOFORMSDURINGDEVELOPMENT
OFBARLEY(HordeumvulgareL.)STEM
L.Begovi1,D.Pavokovi2,S.Mlinari1,A.Lali3,V.Cesar1
1
Department of Biology, J.J. Strossmayer University of Osijek, Osijek, Croatia
(lbegovic@biologija.unios.hr)
2
DepartmentofMolecularBiology,FacultyofScience,UniversityofZagreb,Zagreb,Croatia
3
AgriculturalInstituteOsijek,Osijek,Croatia
Class III peroxidases are involved in many physiological processes in plants. Together with
laccasestheyareinvolvedinoxidationofmonolignolsasthefinalstepinthesynthesisoflignin.
Lignin is a natural polymer that builds the cell wall and provides mechanical strength to the
stem. In the present study, objective was to determine the characteristics of individual
isoforms of peroxidase and laccase in internodes of barley during the four developmental
stages: elongation, heading, anthesis and grain filling. Total peroxidase (tPOD), laccase and
peroxidase covalently bound to the cell wall (cPOD) were extracted and separated using
isolelectric focusing on polyacrylamide gels. Guaiacol was used for detection of peroxidase
activity and syringaldazine for laccase activity. The analysis showed the presence of eight
different isoforms of tPOD with isoelectric point (pI) 810 and one isoform with pI 2.55.
Highest activity was recorded in the first internode in all developmental stages. cPOD were
presentinasingleisoformwithactivityprofilesimilartotPOD.Laccasewererepresentedwith
twoisoforms(pI810andpI2.55)withnodifferenceinactivityinaparticularinternodeand
developmentalstage.Theresultsshowthepresenceandcharacteristicsofthevariousisoforms
ofperoxidaseandlaccaseinvolvedindevelopmentofbarleystem.Futurestudiesaimtoclarify
theroleoftheseenzymesinthebiosynthesisoflignin.
Keywords:internode,cellwall,lignin,isoformes,multicopperoxidase
O93
USPOREDBA GASTROINTESTINALNE STABILNOSTI HRVATSKIH DIVLJIH JESTIVIH BILJAKA:
ANALIZAFENOLNIHSPOJEVA
I.ola1,D.Poljuha2,3,J.Bili3,S.Duda4,T.Bilui5,J.Marki6,G.Rusak1
1
Botaniki zavod, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska(ivana.sola@biol.pmf.hr)
2
InstitutzapoljoprivreduiturizamPore,Pore,Hrvatska
3
Centar za istraivanje materijala Istarske upanije METRIS, Istarska razvojna agencija, Pula,
Hrvatska
4
VeleuiliteuRijeci,OdsjekzaagrikulturuPore,Pore,Hrvatska
5
Kemijskotehnolokifakultet,SveuiliteuSplitu,Split,Hrvatska
6
KlinikibolnikicentarSplit,Split,Hrvatska
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12.HBK10112 CBC
Kvalitativneikvantitativneanalizefenolnihspojevahrvatskihdivljihjestivihbiljakaotrolisne
paroge(AsparagusacutifoliusL.),bodljikaveveprine(RuscusaculeatusL.)iobinogbljuta
(Tamus communis L.) provedene su metodom tekuinske kromatografije visoke moi
razluivanja u sustavu obrnutih faza. Najvea koliina fenolnih spojeva zabiljeena je u
bljutu,anajmanjauveprini.Glikozidiflavonolanajzastupljenijisufenoliubljutuiparogi,
a fenolne kiseline u veprini. Vodeni ekstrakti podvrgnuti su dvofaznoj (eluana i
duodenalna) in vitro digestiji s ljudskim enzimima s ciljem usporedbe gastrointestinalne
stabilnosti glavnih fenolnih spojeva odabranih biljnih ekstrakata. Fenolne kiseline svih
testiranih vodenih ekstrakata bile su potpuno probavljene ve nakon in vitro eluane faze
probave. Od flavonoida, nakon duodenalne probave najbolje su bila ouvana dva glavna
glikozidakempferolaizvodenogekstraktabljuta(50%svaki).Uvodenomekstraktuparoge
otprilike20%kvercetin3Orutinozidaiisoramnetin3Orutinozidajeostalostabilno,doku
ekstraktu veprine nakon duodenalne faze flavonoidi nisu zabiljeeni. S obzirom da bljut
sadrinajveukoliinufenolnihspojevainajveipostotaknjegovihfenolajeouvannakonin
vitro gastrointestinalne probave, zakljuujemo da je za ljudsku prehranu meu istraenim
biljkamabljutnajboljiizvorprirodnihantioksidativnihtvari.
Kljunerijei:RPHPLC,invitrodigestija,kempferol,kvercetin3Orutinozid,izoramnetin3
Orutinozid
I.ola1,D.Poljuha2,3,J.Bili3,S.Duda4,T.Bilui5,J.Marki6,G.Rusak1
1
DivisionofBotany,DepartmentofBiology,FacultyofScience,UniversityofZagreb,Zagreb,
Croatia(ivana.sola@biol.pmf.hr)
2
InstituteofAgricultureandTourismPore,Pore,Croatia
3
MaterialsResearchCentreMETRIS,IstrianDevelopmentAgency,Pula,Croatia
4
PolytechnicofRijeka,AgriculturalDepartmentPore,Pore,Croatia
5
FacultyofChemistryandTechnology,UniversityofSplit,Split,Croatia
6
UniversityHospitalSplit,Split,Croatia
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12.HBK10212 CBC
Keywords:RPHPLC,invitrodigestion,kaempferol,quercetin3Orutinoside,isorhamnetin
3Orutinoside
O94
ASRSLINIPROTEINIOTKRIVENIUINVITROKULTURIKAKTUSAMammillariagracilis
P.Peharectefani1,K.Perica2,K.Landeka1,D.BarZvi3,B.Balen1
1
Zavod za molekularnu biologiju, Bioloki odsjek, Prirodoslovnomatematiki fakultet,
SveuiliteuZagrebu,Zagreb,Hrvatska(ppeharec@zg.biol.pmf.hr)
2
InstitutRuerBokovi,Zagreb,Hrvatska
3
Zavod za prirodne znanosti i Doris i Bertie Black centar za bioenergetiku u prirodnim
znanostima,SveuiliteBenGurion,BeerSheva,Izrael
Kljunerijei:ASRproteini,kaktus,kulturabiljnogtkiva,spektrometrijamasa
ASRLIKEPROTEINSDETECTEDININVITROGROWNCACTUSMammillariagracilis
P.Peharectefani1,K.Perica2,K.Landeka1,D.BarZvi3,B.Balen1
1
Division of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
(ppeharec@zg.biol.pmf.hr)
2
RuerBokoviInstitute,Zagreb,Croatia
3
Department of Life Sciences and The Doris and Bertie Black Center for Bioenergetics in Life
Sciences,BenGurionUniversityoftheNegev,BeerSheva,Israel
Abscisic acid, Stress, and Ripeninginduced (ASR) proteins are plant specific, low molecular
weight,heatstableproteinsthathavehighhydrophilicity.ASRproteinswereshowntopossess
transcriptionfactorandchaperonlikeactivities.Theyareinvolvedinplanttolerancetodrought
and salinity, in regulation of sugar, branched amino acids and cell wall metabolism. The ASR
protein family is widespread in the plant kingdom. However, the Arabidopsis as well as other
BrassicaceaefamilyspecieslackASRgenes,suggestingthatASRproteinsarenotubiquitoustoall
plants.ThereisnoknowledgeifplantsfromtheCactaceaefamily,knownforitshighlytolerance
towaterstress,encodeASRproteins.WethususedinvitrogrownM.gracilistissues(plant,callus
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12.HBK10312 CBC
andtumor)inordertorevealiftheASRlikeproteinscanbefoundinamemberoftheCactaceae
family. ASRproteinscanbeeffectivelypurified tohomogeneity onNiNTAagarose, sincethey
contain an authentic pentahistidine sequence close to their Ntermini. We thus applied ion
chelatingchromatographytopurifyASRlikeproteinfromcactustissues.ElutedproteinsfromNi
agarose were analyzed by means of SDSPAGE, NativePAGE, western blot with antiASR1
antibody and mass spectrometry. These analyses demonstrated, for the first time, that cacti
encode ABA and ripeninginduced proteins. Moreover, we revealed some possible interacting
partnerswithcactusASRlikeproteinswhichneedtobefurtheranalyzed.
Keywords:ASRproteins,cactus,planttissueculture,massspectrometry
3.SimpozijHrvatskogentomolokogdrutva
3rdSymposiumoftheCroatianEntomologySociety
O95
VRETENCA(ODONATA)OTOKAKRKAUGROENOSTISTATUS
T.Bogdanovi1,M.Grgi2
1
Hrvatskoodonatolokodrutvo"Croatocordulia",Valpovo,Hrvatska(tomobogdanov@gmail.com)
2
Vukovarska64,32221Nutar,Hrvatska
Istraivanja faune vretenaca otoka Krka, obavljana su od travnja do rujna 2013 godine na 45
lokaliteta. Koritene su slijedee metode: prikupljanje entomolokim mreicama, obilaenje i
promatranje (metoda linijskog transekta). Kartiranje i digitalna obrada podataka nainjeni su
pomou GIS tehnologije upotrebom ArcView 9.0 programa. Razlika u kvalitativnom i
kvantitativnom sastavu podataka odabranih parametara (brojnost jedinki, vrsta, lokaliteta)
izvrena je pomou klaster analize te ordinacijske metode multidimenzionalnog skaliranja
pomou programa PRIMER 5.0. Koritena je matrica slinosti dobivena izraunavanjem Bray
Curtisindeksaslinosti.Zaklasteranalizukoritenajemetodapovezivanjapoprosjekugrupa,a
ordinacijska MDS metoda provedena je ponavljanjem 100 puta. Ukupno je obraeno 1043
podataka(jedinki)razliitihivotnihstadija(liinke,odraslejedinkeisvlakovi)tejeutvreno40
vrsta,21rod,8porodicai2podredaredaOdonata.Prviputajeutvrenoukupno2novanalaza
ugroenih i 1 nalaz rizinih vrsta za faunu istraivanog podruja. Podaci o rasprostranjenosti
pojedinih vrsta vretenaca i promjenama veliina njihovih populacija, kao i rezultati ekolokih
istraivanja potvruju da su one vrlo osjetljive, te da je opstanak stabilnih populacija izravno
ovisanoovjekovomdjelovanju.Utvrenikvalitativnisastavfaunevretenacanapodrujuotoka
Krka predstavlja 55% ukupne faune vretenaca u Republici Hrvatskoj, to dokazuje znaaj ovog
podrujauzatiti.
Kljunerijei:Vretenca,Krk,ugroenost,zatita
DRAGONFLIES(ODONATA)OFTHEKRKISLANDENDANGERMENTANDSTATUS
T.Bogdanovi1,M.Grgi2
1
Hrvatskoodonatolokodrutvo"Croatocordulia",Valpovo,Croatia(tomobogdanov@gmail.com)
2
Vukovarska64,Nutar,Croatia
SystematicstudiesofdragonfliesontheIslandKrkwerecarriedoutfromApriltoSeptember2013
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12.HBK10412 CBC
at45localities.Thefollowingmethodswereused:themethodofcollectingbyentomologicalnets
and the linear transect methodby strollingand observing.Mappingand digital analyses of the
dataweredonebyGIStechnology,andtheArcView9.0programwasused.Thedifferencewithin
the composition of the qualitative and quantitative data structure (the total number of
specimens,species,andlocality)wasdonebyclusteranalysisandtheordinationmethodofthe
multidimensional scaling of the statistical programme PRIMER 5.0. It was used the BrayCurtis
indexofsimilarity.Fortheclusteranalysisthegroupaveragemethodwasused.Onthebasisof
the1043collectedindividualsatdifferentstagesoflife,atotalof40species,21genera,8families
and 2 suborder of the order Odonata were established. Two endangered, and one vulnerable
speciesaremakinganappearanceforthefirsttimeintheinvestigatedarea.Datarelatingtothe
distributionandfluctuationofspeciesandchangeintheirpopulations aswellastheresultsof
someecoresearchshowthatthedragonfliesareverysensitivespeciesandthatthesurvivalof
stabile population directly depends on the human activity. The established dragonfly species
assemblage on surveyed area represents 55% of the total number of species in Croatia which
provesthisareaasthesignificantintermsofprotection.
Keywords:Dragonflies,Krk,endangerment,protection
O96
SEZONSKA DINAMIKA KOMARACA NA PODRUJU UPANJSKE POSAVINE NAKON POPLAVE
2014.GODINE
I.Kurtek,M.SudariBogojevi,N.Turi,G.Vignjevi,I.Vruina,.Zahirovi,M.Bistrovi,E.Merdi
Odjel za biologiju, Sveuilite Josipa Jurja Strossmayera u Osijeku, Osijek, Hrvatska
(ivan.kurtek@gmail.com)
Nakon katastrofalne poplave u svibnju 2014. godine, podruje upanjske Posavine postalo je
izuzetno povoljno stanite za razvoj velikog broja komaraca budui da se voda nakon
povlaenja i dalje zadrala u inundacijama uz Savu, kanalima, depresijama tla, septikim
jamama i mnogimdrugimumjetnim leglima. Zapotrebeprovoenja javnozdravstvenih mjera
nanavedenompodrujuskrajnjimciljemsmanjenjarizikaodpojaveiirenjavektorskihbolesti,
trebalo je utvrditi kvantitativni i kvalitativni sastav faune komaraca i sezonsku dinamiku
dominantnih vrsta s naglaskom na vrstu Culex pipiens kao potencijalnog prijenosnika virusa
Zapadnog Nila. Istraivanje je provedeno u razdoblju od svibnja do rujna 2014. godine na 16
postaja.ZauzorkovanjeodraslihjedinkikomaracakoritenajemetodaCDCklopkiuzsuhiled
kaoatraktant.Ukupnojeuhvaeno319037jedinkikomaraca,autvrenoje17vrstaodkojih
su tri dominantne: Aedes vexans, Ochlerotatus sticticus i Cx. pipiens. Brojnost komaraca
znaajno je poela rasti tri tjedna nakon poplave i svoj vrhunac dosegla u drugoj polovici
mjeseca lipnja. Tijekom kolovoza pojavila se druga generacija, po brojnosti neto slabijeg
intenziteta. Na pojedinim je postajama broj komaraca bio vrlo velik kroz due vremensko
razdoblje, to ukazuje na injenicu da su komarci dugo uznemiravali stanovnike upanjske
Posavine. Rezultati dobiveni ovim istraivanjem uvelike su doprinijeli uspjenom suzbijanju
komaracanapoplavomugroenimpodrujima.
Kljunerijei:Komarci,upanjskaPosavina,poplava,virusZapadnogNila
I.Kurtek,M.SudariBogojevi,N.Turi,G.Vignjevi,I.Vruina,.Zahirovi,M.Bistrovi,E.Merdi
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12.HBK10512 CBC
DepartmentofBiology,J.J.StrossmayerUniversityofOsijek,Osijek,Croatia(ivan.kurtek@gmail.com)
AfterthedisastrousfloodinMay2014,theterritoryofPosavinanearupanjabecameanideal
mosquitobreedingsitesincewaterafterretreatingstillretainedintheinundationsneartheriver
Sava,inthecanals,grounddepressions,septictanksandmanyotherartificialbreedingsites.In
order to implement public health measures in that area with the aim of lowering the risk of
occurrence and spread of vector diseases, it was necessary to determine the quantitative and
qualitativecompositionofmosquitofaunaandseasonaldynamicsofdominantspecies,withthe
emphasisontheCulexpipiensspeciesasthepotentialvectorofWestNilevirus.Theresearchwas
conductedfromMaytoSeptember2014atsixteenstations.TheCDCtrapmethodwithdryiceas
an attractant was used for sampling of adult specimens of mosquitoes. A total of 319 037
mosquitoes were caught, and 17 species were recognized, with three dominant ones including
Aedes vexans, Ochlerotatus sticticus and Cx. pipiens. The mosquito population has significantly
increasedthreeweeksafterthefloodandreacheditspeakinthesecondhalfofJune.Thesecond
generation emerged during August, but with somewhat lower numbers. At some stations the
mosquito population remained high for a longer time period, which shows that nuisance
mosquitoeshavebotheredtheinhabitantsofPosavinanearupanjaforalongtime.Theresults
ofthisstudyhavecontributedtosuccessfulmosquitocontrolinfloodaffectedareas.
Keywords:Mosquitoes,Posavinanearupanja,flood,WestNilevirus
O97
Epitrix hirtipennis (Melsheimer, 1847) DUHANOV BUHA, NOVI LAN ENTOMOFAUNE
HRVATSKE
E.Raspudi1,M.Brme1,A.Budimir2,Z.Plea2,J.Zdelian2
1
Zavod za zatitu bilja/Poljoprivredni fakultet u Osijeku, Sveuilite Josipa Jurja Strossmayera
Osijek,Osijek,Hrvatska(emilija.raspudic@pfos.hr)
2
Hrvatskiduhanid.d.ViroviticaP.C.Kutjevo,Kutjevo,Hrvatska
Proizvoai duhana imaju sve veih problema u proizvodnji duhana. Prvi vidljivi simptomi
uoeni su na listovima duhana ili u korijenju mladih biljaka. Utvreno je kako se radi o
duhanovombuhauEpitrixhirtipennis(Melsheimer,1847).Kukacveliineoko1,5mm,kreese
skakanjem.Liinkesuveliinedo4,2mm,bjelkastestamnomglavom.Imagopraviteteuvidu
karakteristinihokruglihrupicanalistu,aliinkepravehodnikenakorijenuubiljcidomainu.
Kukacprezimiustadijuimagautluiliuostacimabilja.Javljaseranouproljeezavrijemetoplih
dana.Dobarjeletatelakopronalazibiljkudomaina.tetepraviuklijalitukaoiupolju.E.
hirtipennis se smatra tetnikom duhana, ali moe se hraniti mnogim biljkama iz porodice
Solanaceae.PorijeklomjeizSrednjeiSjeverneAmerike,auEuropiprviputjeotkriven1983u
Italiji. U Hrvatskoj je utvren na povrinama Hrvatskih duhana P.C. Kutjevo te kod njihovih
kooperanata.Upoljujehvatanentomolokommreom,auproizvodnjipresadnicapostavljene
suuteljepljiveploe.Mladubiljkupotrebnojezatititiunajranijojfazirazvojazatosesadnice
prije sadnje potapaju se u 1% otopinu insekticida. Prema literaturi kritini brojevi za E.
hirtipennissu5imaga/biljciranouvegetacijiili510%unitenolisnemase.Osimnaduhanubio
jeprisutaninakrumpiru,alinesmatraseozbiljnotetnikkrumpiraueuropskimzemljamakao
nekedrugevrsteizrodaEpitrixkojesukarantenskezanauzemlju.
Kljunerijei:duhan,duhanovbuha,Epitrixhirtipennis,novilanentomofauneRH
Epitrixhirtipennis(Melsheimer,1847)TOBACCOFLEABEETLENEWMEMBEROFCROATIAN
ENTOMOFAUNA
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12.HBK10612 CBC
E.Raspudi1,M.Brme1,A.Budimir2,Z.Plea2,J.Zdelian2
1
DepartmentofPlantProtection,FacultyofAgriculture,UniversityofJosipJurajStrossmayer,
Osijek,Croatia(emilija.raspudic@pfos.hr)
2
Croatiantobacco,d.d.ViroviticaP.C.Kutjevo,Kutjevo,Croatia
Tobacco producers face major problems in the production of tobacco. The first visible
symptomswereobservedintobaccoleavesortherootsofyoungplants.Itwasdetermined
thatitwastobaccofleabeetleEpitrixhirtipennis(Melsheimer,1847).Insectsizeisabout1.5
mm,anditmovesbyjumping.Thelarvaeareupto4.2mm,white,withadarkhead.Adult
insectmadedamagesbydoingholesintheleafknownas"shotholing",andthelarvaemake
thecorridorsattherootoftheplanthost.Itoverwintersinthestageofimagoinsoilorin
plant residues. Damage can be made in plant bed as well as in the field. E. hirtipennis is
consideredapestoftobacco,butitcanfeedmanyplantsofthefamilySolanaceae.Itsorigin
isfromCentralandNorthAmerica,anditwasfoundforthefirsttimeinEurope,in1983in
Italy.InCroatia,pestisestablishedonfieldsofCroatiantobaccoPCKutjevoandonthefield
oftheirsubcontractors.Thepestwascaughtbyentomologicalnetandintheproductionof
seedlings by yellow sticky traps. Young plant should be protected at an early stage of
development. Before planting seedlings are immersed in 1% solution of the insecticide.
According to the literature, critical numbers are 5 image/plant early in growth or 510%
destroyedpantleaves.Inadditiontotobacco,pestwasalsopresentonthepotato,butisnot
consideredaseriouspestofpotatoesinEuropeancountrieswhilesomeotherspeciesofthe
genusEpitrixarequarantineforourcountry.
Key words: tobacco, tobacco flea beetle, Epitrix hirtipennis, a new member Croatian
entomofauna
O98
RAZNOLIKOSTNONIHLEPTIRASTRAHINJICEIIVANICE
T.Koren1,S.Gomboc2
1
HrvatskoherpetolokodrutvoHyla,Zagreb(koren.toni1@gmail.com)
2
ikovonaselje19,Kranj,Slovenija
Kljunerijei:Natura2000,novinalazi,rijetkevrste
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12.HBK10712 CBC
DIVERSITYOFMOTHFAUNAOFSTRAHINJICAANDIVANICA
T.Koren1,S.Gomboc2
1
CroatianHerpetologicalSocietyHyla,I.Zagreb(koren.toni1@gmail.com)
2
ikovonaselje19,Kranj,Slovenia
During the years 2014 and 2015 moth fauna was surveyed in the Natura 2000 areas
StrahinjicaandIvanica,Krapinskozagorskacounty.Atthe13visited localities,morethan
700mothspecieswererecorded.Thehighestnumberofspecieswasrecordedatthelocalities
StrahinjeRadobojsko(435species),Radoboj,Plat(352species)andthepeakofIvanica(269
species). The species Chersotis rectangula, Nemophora ochsenheimerella, Catoptria
pyramidellus, Udea inquinatalis and Udea cyanalis represent first records for the fauna of
Croatia.Asthehistoricaldataaboutthemothfaunaoftheareaarevirtuallynonexistent,this
surveyrepresentsthefirstsystematicresearchofmothfaunaofStrahinjicaandIvanica.A
very large number of recorded species of moths makes this area one of the best researched
areasinCroatia,andpointstothegreatwealthandimportanceofthesemountainsascentres
ofbiodiversity.Futurestudiesofthesame,aswellasnewhabitatsandlocalitieswilldefinitely
increasethenumberofknownspeciesofbutterfliesofKrapinskozagorskacounty.
Keywords:Natura2000,newrecords,rarespecies
O99
STRUKTURASAPROKSILNIHKORNJAA(COLEOPTERA)NIZINSKIHUMA
M.ag1,N.Turi1,.Zahirovi1,B.Lau2,T.Koren2,M.Zadravec2
1
Odjel za biologiju, Sveuilite Josipa Jurja Strossmayera, Osijek, Hrvatska
(sagmatej@gmail.com)
2
HrvatskoherpetolokodrutvoHYLA,Zagreb,Hrvatska
Saproksilni kornjai predstavljaju kukce usko vezane uz mrtvo drvo. Neodrivo gospodarenje
umama doprinijelo je sve veoj ugroenosti saproksilnih kornjaa te se zbog toga nalaze na
crvenim popisima ugroenih vrsta. Republika Hrvatska oznaena je kao podruje s velikim
brojem ugroenih vrsta od kojih su najee iz porodica strizibuba (Cerambycidae), klinjaka
(Elateridae)ijelenaka(Lucanidae).Ciljovogradajeutvrditibrojnostiraznolikostsaproksilnih
kornjaa unutar razliitih umskih zajednica Kopakog rita te pruiti osvrt na ugroene vrste.
Utvrditeseuinkovitostrazliitihmetodauzorkovanjakojesekoristezapraenjesaproksilnih
kornjaa: metoda prekinutog leta (dizajn pleksiglas i mrea), metoda lovnih klopki i metoda
zrane klopke s atraktantom. Terensko istraivanje bilo je provedeno od travnja do kolovoza
2014. godine, a ukupno je postavljeno 918 klopki tijekom istraivanja. Uzorkovano je 1088
jedinki i 64 vrste iz 14 porodica saproksilnih kornjaa. Najbrojnije porodice bile su
Cerambycidae,CetoniidaeiLucanidetevrsteCetoniaaurata,DorcusparallelipipedusiValgus
hemipterus. Najbrojnija i najraznolikija fauna saproksilnih kornjaa bila je u umi hrasta
lunjaka. Od ukupnog broja na europskoj crvenoj listi se nalazi 20 vrsta, od ega su tri vrste
gotovo ugroene (Cucujus cinnaberinus, Gnorimus variabilis i Lucanus cervus) dok su tri
nedovoljno poznate od kojih je zadnja i ugroena vrsta (Protaetia affinis, Omoglymmius
germari, Rhysodes sulcatus). Najuinkovitija metoda uzorkovanja saproksilnih kornjaa bila je
metodaprekinutogleta.
Kljunerijei:Kornjai,ugroenevrste,metodaprekinutogleta,umskiekosustav
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12.HBK10812 CBC
SAPROXYLICBEETLE(COLEOPTERA)ASSEMBLAGESINLOWLANDFOREST
M.ag1,N.Turi1,.Zahirovi1,B.Lau2,T.Koren2,M.Zadravec2
1
Department of Biology, Josip Juraj Strossmayer University, Osijek, Croatia
(sagmatej@gmail.com)
2
CroatianHerpetologicalSocietyHYLA,Zagreb,Croatia
SaproxylicColeopteraaredeadwoodassociatedbeetles.Theyhavebeenparticularlyaffected
by forest management and make up one of the largest groups of redlisted species in many
countries.TheRepublicofCroatiaisdesignatedasanareawithalargenumberofendangered
species,mostlyfromfamilyCerambycidae,ElateridaeandLucanidae.Theaimofthisstudyisto
determine the abundance and diversity of saproxylic beetles within the different forest
communities in Kopaki rit and to give an overview of endangered species. Likewise, to
compare the efficiency of sampling methods that are primarily used in monitoring studies:
windowflighttrap(crossvanesandsingleplane),pitfalltrapandairtrapwithattractant.The
field research was conducted from April to August 2014 whereas 918 traps were set during
research.Atotalof1088individualsand64speciesfrom14familiesofsaproxylicbeetleswere
caught in this study. The most abundant families were Cerambycidae, Cetoniidae and
LucanidaewiththemostcommonspeciesCetoniaaurata,DorcusparallelipipedusandValgus
hemipterus.Themostabundantanddiverseassemblagesofsaproxylicbeetleswerefoundin
oakwoodforest.Oftotal,20specieswereonEuropeanredlist,threewerenearthreatened
(Cucujuscinnaberinus,Lucanuscervus)andthreeweredatadeficientspeciesofwhichlastone
is also endangered species (Protaetia affinis, Omoglymmius germari, Rhysodes sulcatus). The
mostefficientsamplingmethodwascrossvaneswindowflighttrap.
Keywords:Coleoptera,endangeredspecies,flightinterceptmethod,forestecosystem
O100
VEKTORAedesalbopictusAZIJSKITIGRASTIKOMARACUOSIJEKU,HRVATSKA
I.Vruina1,.Zahirovi1,G.Vignjevi1,E.Merdi1,A.Klobuar2
1
Odjelzabiologiju,SveuiliteJ.J.Strossmayera,Osijek,Hrvatska(iboca@biologija.unios.hr)
2
Nastavnizavodzajavnozdravstvo"Dr.Andrijatampar",Zagreb,Hrvatska
Azijskitigrastikomarac,Aedesalbopictusbiolokijeizdravstvenoznaajnavrstakomarcaije
seirenjeusvijetupratiposljednjihetrdesetgodina.Ovavrloagresivnaitijekomdanaaktivna
vrsta sposobna je prenijeti razliite arboviruse i parazite. Od 2004. godine kada je prvi put
zabiljeenauHrvatskoj,invazivnoseiriHrvatskomdodanas.NakonZagreba,IstreiDalmacije
vrsta je zapoela svoje irenje i na istoni dio Hrvatske. Tijekom 2013. godine, u mjesecu
kolovozu zabiljeen je prvi i jedini nalaz ove vrste na podruju prigradskog naselja Tenja kod
Osijeka,tojebiodovoljanrazlogzaintenzivnijaistraivanja.U2014.,urazdobljuodkolovoza
dostudenog,obavljenasuuzorkovanjajajaovevrstemetodomovipozicijskihklopki.Klopkesu
postavljane na 10 lokaliteta u gradu Osijeku i blioj okolici. Dobiveni rezultati ukazuju da je
tigrastikomaracprisutaniiriseuistonomdijeluHrvatske.
Kljunerijei:Azijskitigrastikomarac,Aedesalbopictus,ovipozicijskeklopke,irenje,Osijek
VECTORAedesalbopictusASIANTIGERMOSQUITOINOSIJEK,CROATIA
I.Vruina1,.Zahirovi1,G.Vignjevi1,E.Merdi1,A.Klobuar2
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12.HBK10912 CBC
Aedes albopictus, asian tiger mosquito is biologically and medically important species of
mosquito, whose distribution has been followed for the last forty years. This species is very
aggressiveandactiveduringthedayandisabletotransmitdifferentarbovirusesandparasites.
Since2004,whenitwasfirstrecordedinCroatia,itcontinuestospreadinvasivelyoverCroatia.
AfterZagreb,IstriaandDalmatiaasiantigermosquitobeganitsexpansiontotheeasternpart
oftheCroatia.InAugust2013,A.albopictuswasobservedforthefirsttime inthesuburban
areaofTenjanearOsijek.Thiswasreasonenoughformoreintensiveresearch.In2014,during
the period from August to November, sampling of eggs was carried out with the oviposition
traps.Trappingwasconductedat10sitesinthecityofOsijekanditssurroundings.Theresults
indicatethatthetigermosquitoispresentandspreadingintheeasternpartoftheCroatia.
KeywordsAsiantigermosquito,Aedesalbopictus,ovipositiontrap,expansion,Osijek
Biologijakopnenihvodaikopna
Biologyoffreshwaterandterrestrialecosystems
IO3
BRYOPHYTE CONSERVATION BIOLOGY IN EUROPE INSIGHTS INTO CONTEMPORARY
ACHIEVMENTS,PROBLEMS,SOLUTIONSANDMANAGEMENT
M.Sabovljevi,A.Sabovljevi
Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Serbia
(marko@bio.bg.ac.rs)
Bryophytesarethesecondlargestgroupofterrestrialplantswith20000speciesworldwide
(2097inEurope)andhighlyneglectedinconservationissues.Manyspeciesremaininjusta
few patches across Europe and were not studied previously. The phylogeography and
molecular data are needed for in vitro propagation, cryopreservation, proper ex situ and
reintroduction i.e. longterm sustainable conservation. Survival can depend as much on
genetic factors such as inbreeding and gene flow as on factors such as population growth
rate and distribution. However, what is a population/individual in bryophytes? The
phenomena such as bottlenecks, inbreeding depression, hybridization, population
subdivision, genetic restoration, invasiveness, reintroductions, fragmentation, mutational
meltdown are poorly documented in bryophytes. Thus, bryophyte conservationists have
quitefewdataatstartpointsandfacesignificantchallenges.Manynewinsightscomesout
during work on specific taxa rescue from extinction. Once the stable production and
propagation in ex situ/captivity condition have been achieved the legislative and
management problems come out. Bryophyte Biology Group in Belgrade (BBGB) has a
collection of 131 bryophytes among which 52% represent redlisted species in European,
regionalornationallevel,BernConventionandEUhabitatdirective.Casestudiesonactive
bryophyteconservation,achievements,problems,solutions,furtheractivitieswillbefurther
presented.
Keywords:bryophytes,conservation,Europe,exsitu,reintroduction
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12.HBK11012 CBC
O101
PROMJENE SASTAVA IHTIOFAUNE RIJEKE SAVE U HRVATSKOJ TIJEKOM POSLJEDNJEG
DESETLJEA(2003.2013.)
M.Piria,T.Tomljanovi,N.prem,D.Matuli,I.Anii,R.Safner,T.Treer
Sveuilite u Zagrebu, Agronomski fakultet, zavod za ribarstvo, pelarstvo, lovstvo i spec.
zoologiju,Zagreb,Hrvatska(mpiria@agr.hr)
Kljunerijei:monitoring,Pontokaspijskiglavoi,nCPUE,multivarijatnaanaliza,diverzitet
ICHTHYOFAUNALCHANGESOFTHERIVERSAVA,CROATIAOVERLASTDECADE(20032013)
M.Piria,T.Tomljanovi,N.prem,D.Matuli,I.Anii,R.Safner,T.Treer
University of Zagreb, Faculty of Agriculture, Department of Fisheries, Beekeeping, Game
ManagementandSpecialZoology,Zagreb,Croatia(mpiria@agr.hr)
DuringtheregularmonitoringoftheRiverSava,organicpollutionatparticularlocationsaswellas
invasive Ponto Caspian gobies (PC gobies) upstream expansion was noted. It is assumed that
these elements could affect the species composition of the Sava. Therefore, an analysis of the
ichthyofaunafrom2003to2013forupstreamand2010to2013forthedownstreamlocations
was performed. nCPUE (catch per unit effort) of 41 fish species caught was used for analysis.
Medsavewastestedseparatelybecauseanthropogenicchangesoftheriverbankagainstflood
protection were observed. Depending on the gradient length, RDA and CCA multivariate data
analysiswasused.ThewaterqualityoftheSavahasnoinfluenceonthefishspeciescomposition,
evenonthelocationwiththelargestorganicpollution.Speciescompositionoftheupperrhitron
(MedsaveandZagreb)isdifferentfromthedownstreamlocations.ThespreadofPCgobieshas
effectonspeciesdiversityandimpactonendangeredspeciessuchasSabanejewiabalcanicaand
Zingel streber is assumed. In the last two years lower ichthyofaunal diversity of the Sava was
observed. At the location Medsave the species composition has been changed, and lower
diversitywasobservedaswell.Continuedmonitoringoftheriverisrecommendedwhichcould
contributetoelucidationoftheecologicalinteractionsthatdeterminethesuccessfulnessofPC
gobiesinvasionindifferentaquaticenvironmentsandtheirinfluenceonnativefishpopulations.
Keywords:monitoring,PontoCaspiangobies,nCPUE,multivariateanalysis,diversity
th
12.HBK11112 CBC
O102
ELEKTRINAPOLJAURBANIPRITISAKNAORGANIZMEUKOPNENIMVODAMA
M.Milia1,D.iki1,I.oli1,D.Grozi1,T.Mandi2
1
Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu, Zagreb, Hrvatska
(mmilisa@inet.hr)
2
Fakultetelektrotehnikeiraunarstva,SveuiliteuZagrebu,Zagreb,Hrvatska
Od mnotva pritisaka kojima ovjek djeluje na svoj okoli, s biolokog stajalita je relativno
najslabije istraen utjecaj elektromagnetskih polja stvorenih elektroenergetskim sustavima.
Vjerojatno jer je taj utjecaj razmjerno nedavno doao u arite panje urbanizacijom i
posljedinoizrazitimrasprostiranjem.Zahvatiuokoliuvezaniuzelektroenergetskesustavesu
meunajbrerastuimurbanimpritiscimanapriroduuEuropi21.stoljea.NaraziniEuropske
unije, koja je ve meu najurbaniziranijim podrujima na svijetu, planirano je priblino 20
postotno proirenje elektroenergetske mree u sljedeih 6 godina. Budui da je relativno
poznat tetan utjecaj elektromagnetskih polja na ljude, osnovni poticaj za istraivanje
potencijalnog utjecaja na ekosustave je izgradnja trajnih izvora elektromagnetskih polja u
prostoru u kojem ih do tada nije bilo. Do sada su vrlo rijetka bila istraivanja utjecaja
elektromagnetskih polja na vodne organizme, a u njima nije razluen utjecaj razliitih
komponenti tih polja. Cilj ovog istraivanja bio je u laboratorijskim uvjetima istraiti i utvrditi
razinu utjecaja elektrinog polja na vodne organizme. Kao ogledne organizime koristili smo
protistekojesmoizlagalielektrinimpoljimaploastihkondenzatora.Utvrdilismopromjeneu
fiziolokom odgovoru odnosno antioksidativnog stresnog enzima superoksid dismutaze te
smanjenjepopulacijeveprikratkotrajnomizlaganjuelektrinompolju.
Kljunerijei:antropogenistres,superoksiddismutaza,Euglena,Paramecium,Hrvatska
ELECTRICFIELDSTHEURBANPRESSUREONFRESHWATERORGANISMS
M.Milia1,D.iki1,I.oli1,D.Grozi1,T.Mandi2
1
Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
(mmilisa@inet.hr)
2
FacultyofElectricalEngineeringandComputing,UniversityofZagreb,Zagreb,Croatia
Humanpressuresonitsenvironmentarenumerous,andfromthebiologicalpointofviewamong
theleastresearchedistheimpactofelectromagneticfieldscreatedbypowersystems.Itcameinto
the focus of attention relatively recently due to increased urbanization and power system
propagation.Constructionofelectricpowersystemsareamongthefastestgrowingenvironmental
pressures in 21st century Europe. At the EU level, which is already among the most urbanized
areasintheworld,about20percentexpansionoftheelectricalnetworkisplannedinthenext6
years.Sincetheadverseeffectsofelectromagneticfieldsonhumansareknown,constructionof
permanentsourcesofelectromagneticfieldsintheareaswheretheyhadnotbeenbeforearethe
main motivation for the research of potential impact on ecosystems. To date, research of the
effectsofelectromagneticfieldsonaquaticlifewasrareandtheydidnotdistinguishtheimpactof
thefieldcomponents.Theaimofthisstudywastostudyanddeterminethelevelofinfluenceof
theelectricfieldonaquaticorganismsinthelaboratory.Weusedprotistsasmodelorganmisms.
Weexposedthemtoelectricfieldsmadebyplatecapacitors.Wehaveidentifiedchangesinthe
physiological response i.e. enzymatic activity of superoxide dismutase and reduction of
populationsaftershorttermexposuretoanelectricfield.
Keywords:anthropogenicstress,superoxidedismutase,Euglena,Paramecium,Croatia
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O103
UTJECAJHIDROLOKOGSTRESANAOBRATAJUNPKRKA
R.MatonikinKepija,M.Milia,M.Ivkovi,Z.Mihaljevi
Zoologijski zavod, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb(rmatonic@biol.pmf.hr)
Kljunerijei:sedrenebarijere,umjetnepodloge,trepetljikai,primarnaprodukcija
INFLUENCEOFHYDROLOGICALSTRESSONPERIPHYTONINKRKANATIONALPARK
R.MatonikinKepija,M.Milia,M.Ivkovi,Z.Mihaljevi
Department of Biology, Zoology, Faculty of Science, University of Zagreb, Zagreb
(rmatonic@biol.pmf.hr)
Periphytonisinvolvedingenesisoftufabarriers,andthusrepresentsoneofbiomediatorsoftufa
deposition. Periphyton development was observed using artificial substrate (glass slides) from
September 2013 to June 2014 on tufa barriers Roki slap (RS) and Skradinski buk (SB) in Krka
National park in two rheotopes (fast and slow current). Overall 98 taxa of heterotrophs were
observed, out of which 64 taxa of ciliates. With an average of 80% ciliates also dominated in
abundance. Contrary to almost identical physicalchemical water parameters measuredon two
barriers,praimaryproductionwashigheronRSsite.Oppositetothat,heterotrophicorganisms
had5to18timeshigherabundanceonSBsitethanthesamerheotopesinRSsite,andthesame
trendwasobservedindiversity.PeritrichiaandSuctoriadominatedbetweenciliatesonSBsite,
while vagile forms of ciliates dominated periphyton in RS site, indicating the influence of
upstreamVisovacLakeoncommunitystructureintufabarrierSB.Peakdischargesduringwinter
andspringledtominorchangesinabundance,butcausedmajorchangesinperiphytonstructure.
While vagile forms and Peritirichia coped well with hydrological stress, there was significant
decreaseinabundanceofSuctoria.Negativeinfluenceofpeakdischargeswasalsoobservedfor
ameboidprotozoaandmostofmeiofaunalgroups.
Keywords:tufabarriers,artificialsubstrate,ciliates,primaryproduction
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O104
ZAKRIVENOST VEGETACIJOM KAO GLAVNI POKRETA STRUKTURE ZAJEDNICE IZVORSKIH
DVOKRILACA
M.Ivkovi1,M.Milia1,V.Baranov2,Z.Mihaljevi1
1
Zoologijski zavod, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska(marija.ivkovic@biol.pmf.hr)
2
LeibnizInstituteofFreshwaterEcologyandInlandFisheries,Berlin,Njemaka
Izvori,aosobitokrkiizvori,sustabilnastanitasastalnimabiotikimfaktorimaistogasemogu
koristiti u brojnim ekolokim eksperimentima. Istraivali smo utjecaj zakrivenosti izvora
kronjama na brojnost, raznolikost i hranidbeni sastav zajednice dvokrilaca (Diptera) dvaju
bliskih reokrenih krkih izvora. Izvori su se prvenstveno razlikovali u pokrovnosti okolne
vegetacije, dok su ostale fizikalnokemijske karakteristike vode bile iste ili sline. est
emergencijskihklopkijebilopostavljenonasvakiodizvorakrozgodinudananasvaprisutna
mikrostanita. Glavna hipoteza naega rada je da e zakrivenost imati najvei utjecaj na
brojnostisastavvrstadvokrilaca.Slinostizmeuizvorajebilasamo37,5%,sa24zajednike
vrste/svojte od73vrste/svojtekojesu naene.Brojnostdvokrilaca jebila8,5xveanaslabo
zakrivenom izvoru, dok su raznolikost i broj vrsta/svojti bili vei na jako zakrivenom izvoru.
Veinavrstasubilidetritivori,anakontogasakupljaiinijebiloprevelikerazlikeizmeuizvora.
Zakljuakovogistraivanjajedazakrivenostvegetacijommoebitiglavnipokretapromjenau
strukturi zajednice dvokrilaca, s brzinom strujanja kao dodatnim faktorom, dok su supstrat i
ostalifizikalnokemijskiimbenicizanemarivi.
Kljunerijei:osvjetljenje,raznolikostvrsta,organskatvar,emergencija,Chironomidae
CANOPYCOVERAGEASAMAINDRIVERINSTRUCTURINGSPRINGDIPTERAASSEMBLAGES
M.Ivkovi1,M.Milia1,V.Baranov2,Z.Mihaljevi1
1
DivisionofZoology,Departmentof Biology, FacultyofScience,UniversityofZagreb,Zagreb,
Croatia(marija.ivkovic@biol.pmf.hr)
2
LeibnizInstituteofFreshwaterEcologyandInlandFisheries,Berlin,Germany
Springs are stable environments with constant abiotic factors and therefore are of use in
variety of ecological experiments. We investigated the influence of canopy coverage on
abundance, diversity, and feeding guilds among Diptera assemblages at two rheocrene karst
springs located near each other. The springs differed by canopy coverage while other
physicochemicalcharacteristicsofthewaterweresimilar.Wesetsixemergencetrapsforone
yearateachspringcoveringallavailablemicrohabitats.Wehypothesizedthatcanopycoverage
will have a strong effect on assemblage composition of Diptera as well as on diversity;
abundance and feeding guilds between sites and that it will have a stronger effect than
microhabitat characteristics. Similarity among springs was only 37.5%, with 24 common
species/taxa out of 73 species/taxa. Abundance of Diptera was 8.5x higher at open canopy
spring,whilediversityandnumberofspecies/taxawashigheratclosedcanopyspring.Majority
ofspeciesweredetritusfeedersfollowedbycollectorsandtherewasnosubstantialdifference
amongsites.Weconcludethatatspringswithsimilarwatercharacteristics,canopycoverageis
the main driver of Diptera assemblage structure, with water velocity as a complementary
factor.Substrateandotherphysicochemicalfactorsareunimportant.
Keywords:Illuminance,speciesdiversity,organicmatter,emergence,Chironomidae
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O105
RAZNOLIKOSTSTANITALITORALNEZONEKAOODREDNICASASTAVAZAJEDNICEPUEVAU
PLITKOMEUTROFNOMJEZERU
J.Lajtner1,I.Zrinak2,M.poljar1,T.Draina1,I.Radanovi1,B.rgar3,A.Ostoji1
1
Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu, Hrvatska
(jasna.lajtner@biol.pmf.hr)
2
Agronomskifakultet,SveuiliteuZagrebu,Hrvatska
3
Vladniksavjetovanjed.o.o.,Zagreb,Hrvatska
Glavniciljovogradabiojeutvrditiutjecajmakrofitskihsastojinanaraznolikost,brojnost,uzrasnu
(veliinsku)itrofikustrukturupuevaurukavcurijekeSutle.Rukavacsesastojioddvabazena,
jednogveeprozirnostisdobrorazvijenimsubmerznimmakrofitimakompleksnoghabitusa,dok
jedrugibazenbezmakrofitaiveemutnoe.Istraivanjejeprovedenotijekomdvijuvegetacijskih
sezona.Tijekomistraivanjadeterminiranoje10vrstapueva,odkojihsuetirivrstezajednike
za oba bazena. U litoralnoj zoni bazena bez makrofitskih sastojina puevi su zabiljeeni
sporadino, s vrlo malom brojnou. Vea brojnost jedinki utvrena je u bazenu s razvijenim
makrofitima,avrstakojajedominiralatijekomistraivanograzdobljajeGyrauluslaevis.Analiza
uzrasne strukture pueva ukazuje da su u kasno proljee i ljeto dominirale juvenilne jedinke, a
udio odraslih jedinki bio je znaajniji tek u jesenskom razdoblju. Od funkcionalnih hranidbenih
skupinapuevanajveiudioimalisustrugaikojisuizvorehranenalaziliuobrataju(perifitonu),
azatimusitnjivai,kojisusehranilisitnimdijelovimamakrofita.Rezultatiovogradaukazujuna
vanost raznolikosti mikrostanita u sastojinama submerznih makrofita koja su puevima
predstavljalaraznolikibogatizvorhrane,sigurnomjestozamrijestteskloniteodpredatora.
Kljunerijei:mekuci,makrofiti,brojnostjedinki,uzrasnastruktura,funkcionalnehranidbeneskupine
J.Lajtner1,I.Zrinak2,M.poljar1,T.Draina1,I.Radanovi1,B.rgar3,A.Ostoji1
1
Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
(jasna.lajtner@biol.pmf.hr)
2
FacultyofAgriculture,UniversityofZagreb,Croatia
3
Vladniksavjetovanjed.o.o.,Zagreb,Croatia
Themainobjectiveofthisstudywastodeterminetheeffectofmacrophytestandsondiversity,
abundance,age(size)andtrophicstructureofsnailsinthebackwateroftheSutlaRiver.The
backwater consists of two pools, one with transparent and welldeveloped submerged
macrophytes with complex habitus, while the other pool is turbid and without submersed
macrophytes.Thestudywasconductedthroughouttwovegetationseasons.Duringthestudy
10speciesofsnailsweredetermined,ofwhichfourspecieswerecommontobothpools.Inthe
littoral zone of the pool without macrophytes, snails have been recorded sporadically and in
very low abundance. Increased abundance of snails was found in a pool with macrophyte
stands,withspeciesGyrauluslaevisbeingdominant.Snailsagestructureanalysesindicatethat
juvenilesdominateinthelatespringandsummer,andtheshareofadultswassignificantin
the fall. From snails functional feeding groups the largest share had scrapers, feeding on
periphyton,thengrazers,feedingonsmallpartsofmacrophytes.Resultsofthisstudyindicate
the importance of the microhabitats diversity in the stands of submerged macrophytes for
snails,becausethosehabitatsprovidediverseandrichsourceoffood,safeplaceforspawning
andshelterfrompredators.
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Keywords:molluscs,macrophytes,abundance,agestructure,functionalfeedingguilds
O106
ISTRAIVAKAAKTIVNOSTDINARSKOGVOLUHARA(DinaromysbogdanoviMartino,1922)
L.Vidati1,M.Ljutina2,I.Ivanek3,D.Lisii1
1
Zavod za animalnu fiziologiju, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska(leavidatic@gmail.com)
2
Dravnizavodzazatituprirode,Zagreb,Hrvatska
3
ZoolokivrtgradaZagreba,Zagreb,Hrvatska
Kompleksnoststanitaimaznaajanutjecajnakoritenjestanita,migracijuiosvajanjenovog
teritorija.Populacijedinarskogvoluhara(DV)sufragmentiraneikompleksnoststanitamogla
biznaajnoutjecatinapovezanostizmeupopulacija.etrnaestjedinkiDVpromatranojekako
bi se prouile njihove bihevioralne i kognitivne karakteristike. Provedena su dva oblika
istraivanjazaprouavanjenjihoveistraivakeaktivnosti.Postavsnovimteritorijemosmiljen
je za promatranje ponaanja u potpuno novom okoliu. ine ga tri varijante razliite
kompleksnosti: otvoreni teritorij, teritorij s nekoliko prepreka i teritorij s mnogo prepreka.
Postavsmirisimaosmiljenjezapromatranjeponaanjauprividnozauzetomteritoriju.inega
etirivarijante:usvakojvarijantiteritorijjeoznaenjednimmirisom,itomirisommujakaili
enkeistevrste,mirisommujakadrugevrste(laboratorijskimi)imirisompredatora(poskok,
Vipera ammodytes). Sva istraivanja su snimana u istom, prethodno odreenom razdoblju
dana, u trajanju od jednog sata. Analizirano je 25 elemenata ponaanja. Uoene su razlike u
ponaanjuovisnookompleksnostivarijanteupostavusnovimteritorijemteovisnoospoluu
postavu s mirisima. Rezultati ovog istraivanja mogu posluiti za procjenu mobilnosti DV u
prirodnomstanituiugroenostiizoliranihpopulacija.
Kljunerijei:ponaanje,kompleksnoststanita,teritorijalnost,meuvrsniiunutarvrsniodnosi,
mobilnost
EXPLORATORYACTIVITYOFMARTINO'SSNOWVOLE(DinaromysbogdanoviMartino,1922)
L.Vidati1,M.Ljutina2,I.Ivanek3,D.Lisii1
1
Departement of Animal Physiology, Faculty of Science, University of Zagreb, Zagreb, Croatia
(leavidatic@gmail.com)
2
StateInstituteforNatureProtection,Zagreb,Croatia
3
ZoologicalGardenofZagreb,Zagreb,Croatia
Habitat complexity has a great influence on habitat utilization, migration and new territory
invasion.Martinossnowvole(MSV)occursinfragmentedpopulationsandhabitatcomplexity
could significantly affect interpopulation connectivity. Fourteen specimens of MSV were
observed to study their behavioral and cognitive characteristics. Two types of experiments
were performed to study their exploratory activity. New Territory Setup was designed to
monitor behavior in a completely new environment. It consisted of three trials of varying
complexity;openfield,fieldwithafewobstacles,andfieldwithmanyobstacles.ScentSetup
wasdesignedtomonitorbehaviorinaseeminglyoccupiedterritory.Itconsistedoffourtrials;
territory was marked with scent of a male or female conspecific, a heterospecific male
(laboratory mouse), and a predator (Vipera ammodytes), respectively. Each trial lasted one
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hour and was recorded during the same predefined period of day. Twentyfive behavioral
elements were analyzed. Differences in behavior were observed depending on setup
complexityintheNewTerritorySetup,anddependingonsexintheScentSetup.Resultsfrom
this study could be used to assess mobility of MSV in natural habitat and to estimate
endangermentofisolatedpopulations.
O107
PRVIPODACIOAKUSTINOMREPERTOARUDINARSKOGVOLUHARA(Dinaromysbogdanovi)
UZATOENITVU
M.Kova1,M.Ljutina1,D.Hamidovi2,M.Brai1,D.Lisii1
1
Zavodzaanimalnufiziologiju,Biolokiodsjek,PrirodoslovnomatematikifakultetSveuilitau
Zagrebu,Zagreb,Hrvatska(matea.kovacc@gmail.com)
2
Dravnizavodzazatituprirode,Zagreb,Hrvatska
Kljunerijei:ultrazvunoglasanje,meuvrsnaiunutarvrsnakomunikacija,Rodentia
FIRSTDATAONTHEACOUSTICREPERTOIREOFBALKANSNOWVOLE(Dinaromysbogdanovi)
INCAPTIVITY
M.Kova1,M.Ljutina1,D.Hamidovi2,M.Brai1,D.Lisii1
1
DepartmentofAnimalPhysiology,DivisionofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(matea.kovacc@gmail.com)
2
StateInstituteforNatureProtection,Zagreb,Croatia
Rodents'vocalisationisanimportantcomponentoftheirbehaviour,andinthecaseoftheBalkan
snow vole (Dinaromys bogdanovi) it is completely unknown. The study on vocalisation of this
endemicrodentwasconductedon15adultspecimenshousedattheZagrebZoo.Theaimofthe
researchwastoidentifytheacousticrepertoireofthisspeciesandtodeterminatedifferencesin
acousticsignalsinarrayofenvironmentalsituations.Theanimalsweretestedinvarioussettings,
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Keywords:ultrasoniccalls,interspeciesandintraspeciscommunication,Rodentia
Toksikologijaiekotoksikologija
Toxicologyandecotoxicology
IO4
KVALITATIVNIPRISTUPEKOTOKSIKOLOKIMISTRAIVANJIMAFORMALNAPOTREBAILINUNOST
B.K.Hackenberger
Zavod za kvantitativnu ekologiju, Odjel za biologiju, Sveuilite Josipa Jurja Strossmayera,
Osijek,Hrvatska(hack@biologija.unios.hr)
Kvalitativna istraivanja su primarna znanstvena istraivanja koja, nakon detekcije problema ili
fenomena, kao rezultat donose hipoteze ili objanjenja njihovih uzroka, mehanizama njihovog
nastanka, te deskripcije njihovih posljedica. Kvantitativna istraivanja nalaze svoje hipoteze u
rezultatimakvalitativnihistraivanjanonjihovciljjekvantifikacijaproblemailifenomenausmislu
generiranjapodatakadostatnihzastatistikuanalizui,unajboljemsluaju,zamatematikiopis
fenomenailiproblema.Potrebapostojanjakvalitetnihkvantitativnihpodatakakojizadovoljavaju
potrebesuvislestatistikeanalizevrloestoseupraksismatrajuformalnompotrebompasena
taj nain statistike metode i koriste. Zbog toga vrlo esto meu dobivenim rezultatima ostaju
skriveni brojni implicitni podaci, fenomeni ostaju opisani na razini deskripcije, a problemi vrlo
esto bivaju rjeavani na krivi nain ili uz bespotrebnu tetu. U okviru ovog predavanja biti e
najprije prikazana sutina koritenja statistikih metoda i matematikih modela kao korisnog i
nunog alata u ekotoksikolokim istraivanjima izvan okvira formalnih potreba. Nadalje, na
nekoliko konkretnih primjera iz prakse, biti e prikazan razvoj ekotoksikolokih pokusa pomou
kvantitativnihmetodaipremoivanjejazaizmeutzv.teoretiaraieksperimentalista.
Kljunerijei:ekotoksikologija,kvalitativnaistraivanja,kvantitativnaistraivanja,matematiko
modeliranje,statistika
B.K.Hackenberger
Subdepartment of Quantitative Ecology, Department of Biology, Josip Juraj Strossmayer
University,Osijek,Croatia(hack@biologija.unios.hr)
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Qualitativeresearchisabasicscientificresearchwhich,afterthedetectionofaproblemora
phenomenon, as a result brings a hypothesis or an explanation of their causes, a
mechanisms of their formation, and descriptions of their consequences. Quantitative
research find their hypothesis in the results of qualitative research but their goal is a
quantification of problems and phenomena in the sense of generating sufficient data for a
statistical analysis and, at best, for a mathematical description of a phenomenon or a
problem. The need for quality quantitative data that meet the needs of a meaningful
statistical analysis in practice is very often being considered a formal need and in such
manner the statistical methods are being used. Therefore, very often among the obtained
results numerous implicit data remain hidden, phenomena remain at the level of mere de
scription and the problems are very often being resolved in a wrong way or with an
unnecessary damage. Within this lecture the essence of usage of statistical methods and
mathematicalmodelsasausefulandnecessarytoolinecotoxicologicalstudiesoutsidethe
framework of a formal requirements will be shown. Furthermore, in a number of practical
examples,thedevelopmentofecotoxicologicalexperimentsusingquantitativemethodsand
bridgingthegapbetweensocalledtheoreticiansandexperimentalistswillbeshown.
O108
UTJECAJPESTICIDANAABLJAJAJA
O.Jovanovi,B.K.Hackenberger,.Lonari,M.Velki,G.Palijan
Odjel za biologiju, Sveuilite Josipa Jurja Strossmayera, Osijek, Hrvatska
(ojovanovic@biologija.unios.hr)
Kljunerijei:Amphibia,Anura,Ranadalmatina,pesticidi
EFFECTSOFPESTICIDESONFROGS'EGGS
O.Jovanovi,B.K.Hackenberger,.Lonari,M.Velki,G.Palijan
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Amphibiansareverysusceptibletovariousthreatsposedbytheenvironment.Sometimes,even
theslightestchangecancausemeasurableshiftsinpopulationdynamics.Environmentalstress,
such as stress caused by pesticides, can result in changes in population characteristics (mostly
negative; decrease of abundance to extinction). Although it is known that pesticides can
negativelyaffectfreshwaterfauna,littleisknownontheeffectsofpesticidesonfrogseggssince
they are generally considered to be well protected by gelatinous substance surrounding the
embryos. Thus, the purpose of our research was to examine the influence of three commonly
used pesticides, dimethoate, glyphosate and pirimiphosmethyl on eggs of agile frog, Rana
dalmatina.Wetestedtheinfluenceofaonetimeexposure,atthebeginningoftheexperiment,
ofenvironmentallyrelevantconcentrationsoftheaforementionedpesticidesontheagilefrogs
eggs,andwemeasuredtimetohatchingandhatchingrate/mortality.Eachpesticidewasusedin
tworeplicates,with200tadpolesineachtank.Wealsomonitoredthesurvivalrateandbodysize
ofhatchedtadpolesincomparisontothecontrolgroup.Thenumberoftadpoleshatcheddiffered
significantlybetweenthepesticidesandthecontrolgroup,butalsobetweendifferentpesticides.
The lowest hatching and survival rate was found with herbicide glyphosate, while insecticide
pirimiphosmethylhadthelowestimpactofalltheexaminedpesticides.
Keywords:Amphibia,Anura,Ranadalmatina,pesticides
O109
SPERMIOTOXICITY AND EMBRYOTOXICITY OF ESBIOTHRIN IN SEA URCHIN Paracentrotus
lividusUSINGSHORTTERMMARINETOXICITYLARVALDEVELOPMENTMETHODS
S.Hayretda1,B.Erkmen2,E.Tac1,F.Erko3
1
anakkale Onsekiz Mart University, Science and Art Faculty, Biology Department,
anakkale,Turkey
2
AksarayUniversity,ScienceandArtFaculty,BiologyDepartment,Aksaray,Turkey
3
GaziUniversity,GaziEducationFaculty,BiologyEducationDepartment,Ankara,Turkey
Ecotoxicological and early life stage embryotoxicity data lacks for widely used synthetic
pyrethroids.Comprehensiveriskassessmentnecessitateslarvaldevelopmentstudieswithmarine
organisms. In this study we evaluated esbiothrin effects on sperm toxicity and embryo
development in model organism the purple sea urchin (Paracentrotus lividus). Six esbiothrin
exposureconcentrationsonspermtoxicityandpluteidevelopmentweretested:10,20,40,65,
100and300g/L.Spermwereexposedfor30min,thenallowedtofertilizeovaduring30minfor
the spermiotoxicity bioassays; evaluation based on fertilization rate as percent fertilization
determinedonarandomsampleof100eggs.Embryotoxicitybioassay:determinedbycomparing
the frequency of normal development and malformations (skeletal deformation and
developmentalretardation)after4872hexposuretoesbiothrinthroughouttheirdevelopment
(pluteusstage)and100larvae/exposureconcentrationwereevaluatedforskeletaldeformations
and undeveloped; compared with the normal. Esbiothrin inhibited fertilization success in a
concentration dependent manner, with IC25 and IC50 values of 17.7825 (CL = 13.695947.1007)
and60.5234(CL=54.897067.2627)g/L,respectively.Asnormalpluteusfrequencydecreased;
anincreaseinmalformationsasskeletaldeformitieswererecorded,esbiothrinishighlytoxicto
embryos than to sperm, and may present a potential risk for the sea urchin in contaminated
marineenvironments.
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Key words: Spermiotoxicity, marine bioassay, embryotoxicity, sea urchin, esbiothrin,
Paracentrotuslividus
O110
DINAMIKARASTAALGINAMEDIJIMASDODATKOMCRVENOGMULJA
A.Oroli1,D.K.Hackenberger1,.Lonari1,V.Feigl2,B.K.Hackenberger1
1
Zavod za kvantitativnu ekologiju, Odjel za biologiju, Sveuilite Josipa Jurja Strossmayera,
Osijek,Hrvatska(ana.orsolic@biologija.unios.hr)
2
DepartmentofAppliedBiotechnologyandFoodScience,BudapestUniversityofTechnology
andEconomics,Budapest,Hungary
Kljunerijei:crvenimulj,uzgojalgi,dinamikarasta,toksinipotencijal,Eiseniafetida
ALGAEGROWTHDYNAMICSONMEDIUMSUPLEMENTEDWITHREDMUD
A.Oroli1,D.K.Hackenberger1,.Lonari1,V.Feigl2,B.K.Hackenberger1
1
Subdepartment of Quantitative Ecology, Department of Biology, Josip Juraj Strossmayer
University,Osijek,Croatia(ana.orsolic@biologija.unios.hr)
2
DepartmentofAppliedBiiotechnologyandFoodScience,BudapestUniversityofTechnology
andEconomics,Budapest,Hungary
12.HBK12112 CBC
depending on the amount of used red mud were measured, as well as changes in toxicity
potentialofthemudmeasuredwithbiochemicalmarkersonearthwormEiseniafetida.The
resultsshowedstatisticallysignificantandmeasurabledifferenceintoxicitypotentialofthe
mixtureofredmudandmediumforalgaegrowth,beforeandafterthecultivationperiodof
21 days. A comparison of algae growth dynamics in the medium with and without the
contentofredmudshowedapplicabilityofredmudintheprocessofalgaecultivation.
Keywords:redmud,algaecultivation,growthdynamics,toxicitypotential,Eiseniafetida
O111
ELECTROMAGNETIC FIELDS AT THE MOBILE PHONE FREQUENCY (900 MHz) TRIGGER THE
ONSETOFTHEGENERALSTRESSRESPONSEALONGWITHDNAMODIFICATIONSINEisenia
fetidaEARTHWORMS
J.P.Bourdineaud1,M.rut2,A.tambuk2,M.Tkalec2,D.Brthes3,K.Malari4,G.Klobuar2
1
UniversityofBordeaux,TalenceCEDEX,France(jeanpaul.bourdineaud@ubordeaux.fr)
2
FacultyofScience,DepartmentofBiology,UniversityofZagreb,Zagreb,Croatia
3
Institute of Cell Biochemistry and Genetics, UMR 5095, CNRS, University of Bordeaux,
BordeauxCedex,France
4
FacultyofElectricalEngineeringandComputing,UniversityofZagreb,Zagreb,Croatia
Eisenia fetida earthworms were exposed to electromagnetic field (EMF) at the mobile phone
frequency(900MHz),andatfieldlevelsrangingfrom10to120Vm1foraperiodof2h.Potential
effects of longer exposure (4 h), field modulation, and recovery period of 24 h after 2 h of
exposure were addressed at the field level of 23 V m1. All exposure treatments induced
significantDNAmodificationsasassessedbyaquantitativerandomamplifiedpolymorphicDNA
PCR.Evenafter24hofrecoveryfollowinga2hexposure,thenumberofprobehybridizationsites
displayedasignificant2folddecreaseascomparedtountreatedcontrolearthworms,implyinga
lossofhybridizationsites.Expressionofgenesinvolvedintheresponsetogeneralstress(HSP70
andMEKK1,thelatterinvolvedinsignaltransduction),oxidativestress(CAT,encodingcatalase),
chemical and immune defence (LYS, encoding lysenin, and MYD, encoding a myeloid
differentiationfactor)wereupregulatedafterexposureto10andmodulated23Vm1fieldlevels.
WesternblotsshowinganincreasedquantityofHSP70andMTCO1proteinsconfirmedthisstress
response(MTCO1isthesubunit1ofcytochromecoxidase,indicatingamitochondrialimpact).
HSP70andLYSgeneswerefoundupregulatedafter24hofrecoveryfollowinga2hexposure,
meaningthattheeffectofEMFexposurelastedforhours.
O112
ANALIZA PAH METABOLITA U UI ODABRANIH KOMERCIJALNIH RIBA DU ISTONE
JADRANSKEOBALE
B.Hamer1,A.Barievi1,N.Medi2,V.S.Perusco1
InstitutRuerBokovi,Centarzaistraivanjemora,Rovinj,Hrvatska(hamer@cim.irb.hr)
SveuiliteJurjaDobrileuPuli,Pula,Hrvatska
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12.HBK12212 CBC
Kao biomarkeri izloenosti nafti i kao mogui pokazatelj kvalitete morskog okolia analizrani su
metaboliti policiklikih aromatskih ugljikovodika (PAH) u ui komercijalnih vrsta riba Mugil
cephalus,SarpasalpaiMullusbarbatussapostaja:Ploe(F2),Split(F4),ibenik(F5),Rijeka(F8)i
Rovinj(F9).Ribesukupljenesvjeenalokalnimribarnicamasiregistraivanogpodrujajerihje
bilonemogueulovitinalokacijamakojesuispitivaneuokvirupilotprojektaJadranII.Vrsteriba
su odabrane prema hranidbenoj poziciji: M. cephalus (vodeni stupac, svejed), S. salpa (vodeni
stupac, biljojed) i M. barbatus (dno, detritofag). Osim morfolokih pokazatelja za identifikaciju
jedinki primijenjena je i analiza cox1 gena (barcoding). PAH metaboliti su analizirani pomou
fluorimetra i fluorescencije fiksnih valnih duljina (FF): 290/335, 341/383 i 380/430 nm
(pobuda/emisija). FF 290/335, preteno mjeri naftalenske vrste PAH metabolita, benzo[a]piren
vrstemetabolitanajboljesudetektiranenaFF380/430,aFF341/383uglavnommjerimetabolite
pirena.RezultatipokazujupretenoizloenostribanaftalenvrstimetabolitaFF290/335(30491),
s vrijednostima cca. 1030 puta viim za od ostale dvije vrste metabolita FF341/383 (246) i
FF380/430 (144). Nadalje, ukupne vrijednosti PAH metabolita svih triju vrsta riba rangirale su
istraivana podruja obzirom na izloenost riba nafti, odnosno policiklikim aromatskim
ugljikovodicimakakoslijedi:F5>F9>F4>F2>F8.
Kljunerijei:PAH,ribe,Jadranskomore,nafta,oneienje
B.Hamer1,A.Barievi1,N.Medi2,V.S.Perusco1
1
RuerBokoviInstitute,CenterforMarineResearch,Rovinj,Croatia(hamer@cim.irb.hr)
2
JurajDobrilaUniversityofPula,Pula,Croatia
BilePAHmetabolitecontentinselectedcommercialspeciesMugilcephalus,Sarpasalpaand
Mullusbarbatuspurchasedfromlocalfishmarkets:Ploe(F2),Split(F4),ibenik(F5),Rijeka
(F8)andRovinj(F9)wereanalyzedasabiomarkerofexposuretooilandpossibleindicatorof
marine environment quality. Local fish markets were used because it was not possible to
cathchosenspeciesatsitesinvestigatedintheframeofpilotProjectAdriaticII.Fisheswere
selected according food web positions: M. cephalus (water column, omnivore), S. salpa
(watercolumn,herbivore)andM.barbatus(benthic,detritofag).Theanalysisofcox1gene
was applied as tool for fish specimens identification (barcoding). PAH metabolites were
analyzed in bile by means of fixed wavelength fluorescence (FF) measurement using
fluorimeter excitation/emission wavelength pairs 290/335, 341/383, and 380/ 430 nm. By
FF290/335, mainly naphthalene type of metabolites, typically associated with petroleum
productsaredetected,benzo[a]pyrenetypeofmetabolitesarebestdetectedbyFF380/430
andbyFF341/383mainlypyrenederivedmetabolites.Resultsindicatemostlynaphthalene
type fish exposure cca. 1030 times higher values for FF290/335 (30491), than two other
types FF341/383 (246) and FF380/430 (144). Further, total sum values of all three fish
speciesrankedfishcontaminantexposureatinvestigatedareasasfollows:F5>F9>F4>F2>F8.
Keywords:PAHmetabolites,fish,AdriaticSea,oil,pollution
O113
ALERGENI GRINJA, OHARA I PLIJESNI U SEDIMENTIRANOJ PRAINI U KUANSTVU U
HRVATSKOJ
Lj.Prester,J.Macan
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12.HBK12312 CBC
Jedinicazamedicinuradaiokolia,Institutzamedicinskaistraivanjaimedicinurada,Zagreb,
Hrvatska(prester@imi.hr)
Svrharadabilajeustanovitirazinealergenaunutarnjihprostorakojisupovezanisazdravstvenim
rizicima.Usedimentiranojprainiodredilismokoncentracijealergena:grinjaDermatophagoides
pteronyssinus(Derp1)iDermatophagoidesfarinae(Derf1),oharaBlatellagermanica(Blag1),
teplijesniAlternariaalternata(Alta1)iAspergillusfumigatus(Aspf1).RazinaalergenagrinjaDer
p1iDerf1veaod2g/gprainepovezanajesalergijskomsenzibilizacijom,arazinaveaod10
g/g s klinikim respiratornim simptomima. Sakupljeno je 30 uzoraka sedimentirane praine
uzorkovane u kuanstvima sa zagrebakog podruja. Nakon ekstrakcije, alergeni su odreeni
standardnomenzimimunokemijskommetodom(ELISA)(IndoorBiotechnologiesLtd,Cardiff,UK).
Alergenigrinjeustanovljenisuu75%kuanstava.KoncentracijaDerp1bilajeurasponuod0,1
12,5 g/g, a Der f 1 od 0,103,2 g/g. Koncentracija Der p 1 + Der f 1 > 2 g/g, povezana s
poveanimrizikomzasenzibilizaciju,ustanovljenajeu27%kuanstva,aBlag1umanjeod3%
kuanstva.AlergeniplijesniAlta1iAspf1nisuustanovljeninitiujednomuzorkukunepraine
(ispod granice detekcije metode). Zakljuuje se da su alergeni grinja najei alergeni u
kuanstvimazagrebakogpodruja.
Kljunerijei:alergeniunutarnjihprostora,grinjekuanskepraine,ohari,plijesni,Derp1
Lj.Prester,J.Macan
Occupational Health and Environmental Medicine Unit, Institute for Medical Research and
OccupationalHealth,Zagreb,Croatia(prester@imi.hr)
Weevaluatedthelevelofmainallergensofindoorenvironmentassociatedwithhealtheffects
in man. We have determined the level of dust mites allergens Der p 1 (Dermatophagoides
pteronyssinus) and Der f 1 (Dermatophagoides farinae), cockroach allergen Bla g 1 (Blatella
germanica), and mould allergens Alt a 1 (Alternaria alternata) and Asp f 1 (Aspergillus
fumigatus).FormiteallergensDerp1andDerf1athresholdlevelsof2and10g/gofdust
have been proposed for development of allergic sensitization and respiratory symptoms,
respectively. A total of 30 dust samples were collected in urban homes in Zagreb. After
extraction, all allergens were detected by using standard enzyme immunoassays (ELISA)
(Indoor Biotechnologies Ltd, Cardiff, UK). Mite allerges were detected in 75% of households.
TheconcentrationofDerp1andDerf1wereintherange0.112.5g/gand0.103.2g/g,
respectively. While the levels of Der p 1 + Der f 1 >2 g/g associated with increased risk of
sensitizationwerefoundin27%ofhomes,thisriskwasfoundinlessthan3%ofdustsamples
forcockroachallergenBlag1.MoldallergensAlta1andAspf1werenotdetectedinanydust
samples(lowerthanlimitdetectionofmethod).Theresultssuggestthatmiteallergensarethe
mostcommonindoorallergensinhouseholdsinZagrebarea.
Keywords:indoorallergens,housedustmites,cockroaches,moulds,Derp1
O114
SINERGISTIKI UINCI VAZODILATACIJSKIH I ANTIKOAGULACIJSKIH LIJEKOVA NA
OKSIDATIVNISTRESUMOZGU
J.Krasi,D.iki
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12.HBK12412 CBC
Zavodzaanimalnufiziologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuiliteu
Zagrebu,Zagreb,Hrvatska(domagoj.djikic@biol.pmf.hr)
Kljunerijei:mozak,oksidativnistres,antikoagulativniivazodilatacijskilijekovi
J.Krasi,D.iki
DivisionofAnimalPhysiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(domagoj.djikic@biol.pmf.hr)
Previoustoxicologicalresearchshowedthatbloodbrainbarrierandbrainvascularsystemare
highly susceptible to the effects of pharmaceuticals with vasodilatation or anticoagulative
properties.Theadverseeffectsoncellularoxidativestresscausedbyoverthecounter(OTC)of
household drugs (acetylsalicylic acid), herbal remedies and pills (for example gingko) or
prescriptiondrugs(warfarin)arewelldescribed.Muchlessisknownaboutmutualinteraction
betweenthesesubstancesonoxidativestresslevelsespeciallyinthebrain.Withinthisstudy
Y59 rats were treated daily with corresponding recommended doses and combinations of
acetylsalicylicacid,ginkgoandwarfarineduring15days.Fourmarkersofoxidativestresswere
assessed; superoxide dismutase (SOD), catalase (CAT), gluthathione (GSH) and
malondialdehyde(MDA)invariousbrainregions.Theresultshaveshownchangesofoxidative
stress levels in prefrontal cortex, cortical regions and cerebellum of exposed animals. These
changesimplicatesynergisticandpotentiatedtoxicpotentialofcombinationsofnameddrugs.
Keywords:brain,oxidativestress,vazodilatationalandanticoagulativedrugs
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Mikrobiologija
Microbiology
O115
KOMPARATIVNA ANALIZA GENOMA FITOPLAZMI: OD NESTABILNOSTI GENOMA DO
PRILAGODBENADOMAINE
M.erugaMusi,A.Slovi,M.Pinteri
Zavod za mikrobiologiju, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u
Zagrebu,Zagreb,Hrvatska(martina.seruga.music@biol.pmf.hr)
Fitopatogenebakterijeroda'CandidatusPhytoplasma'malihsugenomakojisukrozdinaminu
koevoluciju s biljnim domainima i kukcimavektorima doivjeli specifine gubitke i dobitke
gena.Jouvijekihnijemogueuzgojitiuistojkulturiinvitro.Unatotome,upotpunostisu
sekvencirana i dostupna 4 fitoplazmatska genoma uz nekolicinu nepotpunih. Iako reducirani,
genomi fitoplazmi bogati su ponavljajuim sljedovima. Ponavljajui sljedovi nazvani
potencijalne pokretne jedinice (potential mobile units; PMUs) nalik su replikativnim
transpozonima i mogu postojati kao kruni ekstrakromosomski elementi s poveanom
ekspresijom u kukcima u odnosu na biljke. PMUs doprinose varijacijama u veliini te visokoj
plastinostigenomaiprilagodbinarazliitedomaine.Jedanodponavljajuihgenajestigen
ssb, Samo jedna kopija gena ssb u genomu im a drugaije evolucijsko porijeklo, nalazi se u
specifinom genomskom okruenju te se smatra izvornom kopijom. Izvorna kopija gena ssb
izolatavrsta'Ca.P.asteris'i'Ca.P.solani'umnoenajeisekvencirana.Filogenetskomanalizom
pokazanajeouvanostizvornekopijegenassb,uzizuzetakvrste'Ca.P.mali'tojeuskladus
posebnom organizacijom i stabilnou genoma ove fitoplazme. Metodom Southern blot, u
genomu 'Ca. P. solani' dokazana je prisutnost jo jedne kopije tog gena, to ukazuje na
mogunost postojanja ekstrakromosomskog elementa karakteristinog za ovu vrstu iji je
genomnajveiinajnestabilnijimeufitoplazmama.
Kljunerijei:fitoplazma,genomika,filogenija,genom,biljnipatogen
M.erugaMusi,A.Slovi,M.Pinteri
Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
(martina.seruga.music@biol.pmf.hr)
Insecttransmitted bacteria from the genus 'Candidatus Phytoplasma' have small genomes that
experienced specific gene losses and gains through their dynamic coevolution with plant and
insect hosts. Axenic cultivation of these bacteria is still challenging. However, a number of
genome drafts and 4 fully annotated genomes are available. In spite of being reduced,
phytoplasma genomes are repeatrich and contain multicopy genes. Repeats termed potential
mobile units (PMUs) have characteristics of replicative transposons and may exist as circular
extrachromosomal elements with enhanced expression in insects compared with plant hosts.
PMUs contribute to phytoplasma genome size variations and high plasticity as well as to
adaptation to diverse hosts. One of the multicopy genes is the ssb gene with only one copy
locatedinaspecificgenomicneighbourhoodhavingadifferentevolutionaryorigin.Inthisstudy,
ssb gene was amplified and sequenced from a number of 'Ca. P. asteris' and 'Ca. P. solani'
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isolates. Phylogenetic analyses have shown that all original ssb genes from different
phytoplasmasclusteredtogether.Theexceptionwasthe'Ca.P.mali'singlessbgene,whichisin
accordance with its' different genome organization and stability. Southern blot analyses have
revealed another copy of the ssb gene in 'Ca. P. solani', suggesting the presence of an
extrachromosomalelementcharacteristicofthespecieshavingthelargestandthemostunstable
genomeamongphytoplasmas.
Keywords:phytoplasma,plantpathogen,genomics,genome,phylogeny
O116
MOLEKULARNATIPIZACIJAUZRONIKAFILODIJEULJANEREPICE
D.kori,M.erugaMusi,S.erni
Bioloki odsjek, Prirodoslovnomatematiki fakultet Sveuilita u Zagrebu, Zagreb, Hrvatska
(dijana.skoric@biol.pmf.hr)
Filodija uljane repice (Brassica napus ssp. oleifera (DC.) Metzg.) je bolest koja izaziva velike
gubitkeidosadajebilapovezivanasazarazomfitoplazmama(rod'CandidatusPhytoplasma').
BiljkeprikupljenenapodrujuZagrebaimalesu,tipinozasindromfilodije,virescenciju,male
deformirane mahune i reduciran broj sjemenki. Takoer su zabiljeene nekroze stabljika i
listova, te kloroze listova tipine za infekciju virusom mozaika repe (Turnip mosaic virus;
TuMV). Kako bi se otkrio uzronik bolesti, provedena je molekularna tipizacija fitoplazmi i
virusa. Bioloka i molekularna istraivanja otkrila su infekciju TuMVom, a izolat CRO184A
klasificiran je u skupinu worldB. Filogenija nije pokazala tragove rekombinacija. PCRRFLP
analizom i sekvenciranjem 16S rRNA oekivano je potvrena prisutnost fitoplazme "aster
yellows" (AY, 'Ca. P. asteris'), soja iz ribosomske podskupine 16SrIB. Multigenska analiza
konzerviranihgena tufB, secY, groEL i ribosomskihproteinskihgena te varijabilnoggena amp
potvrdila je klasifikaciju fitoplazme. Gen amp kodira AYspecifini imunodominantni
membranskiprotein,apromjeneunjemuukazujunadrukijuprenosivostkukcimavektorima
tj.prilagodbenovomtipuvektora.Molekularnomtipizacijompoprviputajedetaljnoopisana
fitoplazmauzronik filodije uljane repice, a takoer je prvi put dokazana koinfekcija repice
fitoplazmom i virusom. Ova studija predstavlja osnovu za daljnja istraivanja njihovih
sinergistikihuinaka.
Kljunerijei:'CandidatusPhytoplasmaasteris',genom,MLST,virusmozaikarepe
MOLECULARTYPINGINDECIPHERINGETIOLOGYOFRAPEPHYLLODYDISEASE
D.kori,M.erugaMusi,S.erni
Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
(dijana.skoric@biol.pmf.hr)
Rapephyllody diseaseassociatedwithphytoplasmascandecimatetheoilseedrape(Brassica
napus ssp. oleifera (DC.) Metzg.) production. Plants sampled in Zagreb area had typical rape
phyllody syndrome including virescence, small deformed siliques and seed reduction. Also,
atypicalstemnecrosis,leafchloroticspotsandnecroticchangesreminiscentofaTurnipmosaic
virus(TuMV)infectionwererecorded.Moleculartypingwasperformedaimedatcharacterizing
agentsinvolvedinthisrapephyllodyoutbreak.Biologicalandmolecularinvestigationsrevealed
theinfectionwithTuMVandtheisolateCRO184AwasclassifiedintotheworldBphylogenetic
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lineage. No evidence for interlineage recombination was obtained. PCRRFLP and 16S rRNA
gene sequencing identified an aster yellows phytoplasma (AY, 'Candidatus Phytoplasma
asteris')strainofthe16SrIBsubgroup.Multigenesequencecharacterizationofhousekeeping
tufB, secY, groEL and ribosomal protein genes plus a highly variable amp gene confirmed its
affiliation.TheAYspecificampgeneencodinganimmunodominantmembraneproteinshowed
significantvariabilitysuggestingalteredinsectvectorspecificityandtransmissibility.Molecular
typingprovidedthefirstdetailedcharacterizationof'Ca.P.asteris'strainassociatedwiththe
rapephyllodydisease.Also,thiswasthefirstrecorded'Ca.P.asteris'andTuMVcoinfectionin
oilseedrape.Thisresearchlaysfoundationforinvestigatingtheirpossiblesynergisticeffects.
Keywords:'CandidatusPhytoplasmaasteris',MLST,Turnipmosaicvirus,genome
O117
RAZNOLIKOSTGLJIVECryphonectriaparasiticaUZARASTAJUIMKALUSIMAKESTENA
V.Karin1,M.Jei1,M.Idojti2,M.urkoviPerica1
1
Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu, Zagreb, Hrvatska
(vala255@hotmail.com)
2
Zavodzaumarskugenetiku,dendrologijuibotaniku,umarskifakultet,SveuiliteuZagrebu,
Zagreb,Hrvatska
Kljunerijei:Cryphonectriaparasitica,rakkestenovekore,Cryphonectriahypovirus1(CHV1),
zarastajuirak
DIVERSITYOFCryphonectriaparasiticaINHEALEDCHESTNUTCANKERS
V.Karin1,M.Jei1,M.Idojti2,M.urkoviPerica1
1
Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
(vala255@hotmail.com)
2
Department of Forest Genetics, Dendrology and Botany, Faculty of Forestry, University of
Zagreb,Zagreb,Croatia
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Cryphonectriaparasiticaisapathogenicfungusthatcauseschestnutblight.Diseasedchestnuts
recover naturally if the fungus which caused the canker is infected with Cryphonectria
hypovirus1(CHV1).Thisviruscausestheconversionoffungusfromvirulentintohypovirulent
andthusenablesbiologicalcontrolofthedisease.CHV1isdsRNAviruswithoutacapsid.The
presenceofthisdsRNAinthemyceliumreducesvirulenceandreproductivecapacitiesofthe
fungus. If CHV1 is introduced into an active canker caused by virulent C. parasitica strain,
cankerexpansionceasesandcallus(healingcanker)isformed.Therefore,itisimportantthat
hypovirulent strains persist in C. parasitica populations. The aim of this research was to
evaluatethediversityofC.parasiticapopulationsinhealedcankersinOzaljandMarkuevacin
Zagreb. Bark samples were collected from 24 heal ed cankers; from callus tissue and callus
woodinterfacepureC.parasiticacultureswereisolated.ThepresenceofCHV1wasconfirmed
bydsRNAisolationandRTPCR.It is interesting that virulent C.parasitica strains werefound
quite frequently in healed cankers, although hypovirulent strains are usually associated with
healing. We suspect that this is the result of either spontaneous loss of the virus from
hypovirulent fungal strain, which previously caused healing or colonization of the callus by
new,virulent,fungalstrainswhichcouldreinitiatethediseaseonthehealedcankers.
Keywords:Cryphonectriaparasitica,chestnutblight,Cryphonectriahypovirus1(CHV1),healedcanker
O118
OPSTOJNOST VIRUSA Cryphonectria hypovirus 1 MOE OVISITI O NJEGOVOM PRIJENOSU
IZMEURAZLIITIHTIPOVAVEGETATIVNEKOMPATIBILNOSTIGLJIVECryphonectriaparasitica
L.Nuskern1,Z.Katani2,Lj.Krstin2,M.Jei1,M.urkoviPerica1
1
Zavod za mikrobiologiju, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u
Zagrebu,Zagreb,Hrvatska(mirna.curkovicperica@biol.pmf.hr)
2
OdjelzaBiologiju,SveuiliteJosipaJurjaStrossmayera,Osijek,Hrvatska
Cryphonectria parasitica, fitopatogena gljiva koja uzrokuje rak kestenove kore znaajno je
unitilasastojinepitomogakestenauEuropi.GljivajedomainvirusaCryphonectriahypovirus
1kojiomoguujeprirodnubiokontroluovogpatogena.Uestalinainprijenosavirusaizmeu
razliitih sojeva gljive je putem anastomoze hifa. Mogunost anastomoze regulirana je
sustavom vegetativne kompatibilnosti: prijenos virusa najuspjeniji je izmeu micelija istog
vegetativnog (vc) tipa, dok je izmeu micelija razliitih vc tipova otean. U laboratorijskim
uvjetimaprenijelismorazliitesojevevirusauizolategljiverazliitihvctipova.Zamijetilismo
dajeopstojnostvirusaumicelijuakceptoruovisilaotomepripadajulidonorskiiakceptorski
micelijistomilirazliitomvctipu.Pretpostavljamodadogubitkavirusaizmicelijadolazizbog
nedovoljneprilagodbenanovogdomainakojijedrugaijegvctipa.Rezultatidobiveniinvitro
ne moraju nuno implicirati isti proces u prirodi, ali ini se da se ovi rezultati podudaraju s
rezultatimaterenskihistraivanjaukojimajezamijeenodaukalusima,rakranamazaraslima
podutjecajemgljivezaraenevirusom,estonakonnekogvremenavienenalazimovirus.
Kljunerijei:biolokakontrola,invazivnavrsta,virulentnost
PERSISTENCEOFCryphonectriahypovirus1INCryphonectriaparasiticaMIGHTBEAFFECTED
BY THE TRANSFER BETWEEN THE DIFFERENT VEGETATIVE COMPATIBILITY TYPES OF THE
HOST
L.Nuskern1,Z.Katani2,Lj.Krstin2,M.Jei1,M.urkoviPerica1
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Keywords:biologicalcontrol,invasivespecies,virulence
Komparativnafiziologija,imunobiologijaibiologijaovjeka
Comparativephysiology,immunobiologyandhumanbiology
IO5
PREDVIANJEISHODINIHSTANICARAKANATEMELJUORGANIZACIJEKROMATINA
Rakjebolestpotenciranasomatskimmutacijama,kojenisujednolikodistribuiranedugenoma
staniceraka,vesepojedinegenomskeregijerazlikujuulokalnojgustoisomatskihmutacija.
Pokazano je da je organizacija kromatina jedna od glavnih odrednica genomske raspodjele
somatskih mutacija u stanicama raka. Kako su i somatske mutacije i organizacija kromatina
tkivno specifine pojave, koristei metode raunalne biologije usporedili smo raspodjelu
mutacijauvieneovisnihtipovarakasbrojnimepigenetskimpodacimamjerenimurazliitim
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12.HBK13012 CBC
normalnimtkivima.Pokazalismodaepigenetskasvojstvaivrijemereplikacijeobjanjavajudo
86%varijanceuraspodjeligustoemutacijadugenomarazliitihtipovaraka.Najboljatonost
predvianjalokalnegustoesomatskihmutacijadobivenajeupotrebomepigenetskihsvojstava
stanicaukojimajeprouavanitiprakanajvjerojatnijenastao.Uzto,otkrilismodajemogue
tonije predvidjeti raspodjelu mutacija u raku na temelju kromatinske strukture stanica u
kojima je rak nastao nego na temelju kromatinske strukture odgovarajuih staninih linija.
Nadalje, pokazali smo da se u skoro 90% sluajeva tip stanica iz kojih je rak nastao moe
precizno odrediti na temelju raspodjele mutacija uz njegovu genomu. Dakle, nai rezultati
pokazuju da genom stanica raka obuhvaa mnotvo informacija o identitetu i epigenetskim
obiljejimastanicaukojimajepojediniraknastao.Tajpodatak,pak,moebitiizuzetnovaanu
dijagnoziitretmanumalignihbolesti.
Kljunerijei:epigenetskaobiljeja,genom,rak,somatskemutacije
USINGCHROMATINORGANIZATIONTOPREDICTCANCERCELLOFORIGIN
R.Karli1*,P.Polak2,3*,A.Koren3,4,R.Thurman5,R.Sandstrom5,M.S.Lawrence3,A.Reynolds5,
E.Rynes5,K.Vlahoviek1,7,J.A.Stamatoyannopoulos5,6,S.R.Sunyaev2,3
(*equallycontributed)
1
Bioinformatics Group, Division of Molecular Biology, Department of Biology, Faculty of
Science,UniversityofZagreb,Zagreb,Croatia
2
Division of Genetics, Department of Medicine, Brigham & Womens Hospital and Harvard
MedicalSchool,Boston,Massachusetts,USA
3
TheBroadInstituteofMITandHarvard,Cambridge,Massachusetts,USA
4
DepartmentofGenetics,HarvardMedicalSchool,Boston,Massachusetts,USA
5
DepartmentofGenomeSciences,UniversityofWashington,Seattle,Washington,USA
6
Department of Medicine, Division of Oncology, University of Washington, Seattle,
Washington,USA
7
DepartmentofInformatics,UniversityofOslo,Oslo,Norway
Cancer is a disease potentiated by mutations in somatic cells, which are not distributed
uniformlyalongthehumangenome.Instead,differenthumangenomicregionsvarybyupto
fivefold in the local density of cancer somatic mutations. Epigenomic organization has been
proposedasamajordeterminantofthecancermutationallandscape.However,bothsomatic
mutagenesis and epigenomic features are highly celltypespecific. We investigated the
distribution of mutations in multiple independent samples of diverse cancer types and
compared them to celltypespecific epigenomic features. Here we show that chromatin
accessibility and modification, together with replication timing, explain up to 86% of the
variance in mutation rates along cancer genomes. The best predictors of local somatic
mutationdensityareepigenomicfeaturesderivedfromthemostlikelycelltypeoforiginofthe
correspondingmalignancy.Moreover,wefindthatcelloforiginchromatinfeaturesaremuch
strongerdeterminantsofcancermutationprofilesthanchromatinfeaturesofmatchedcancer
cell lines. Furthermore, we show that the cell type of origin of a cancer can be accurately
determinedbasedonthedistributionofmutationsalongitsgenome.Thus,theDNAsequence
ofacancergenomeencompassesawealthofinformationabouttheidentityandepigenomic
featuresofitscelloforigin.Thisinformationcanbeimportantfortheaccuratediagnosisand
treatmentofmalignantdiseases.
Keywords:cancer,epigenomicfeatures,genome,somaticmutation
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O119
REPLIKACIJAUBSTANINIMLIMFOMIMA
V.Tadi,P.BaiPalkovi,B.Sasi,M.Klasi,P.Kora
Zavodzamolekularnubiologiju,Biolokiodsjek,Prirodoslovnomatemtikifakultet,Sveuilite
uZagrebu,Zagreb,Hrvatska(petra.korac@biol.pmf.hr)
Kljunerijei:Bstaninilimfomi,replikacija,kromatin
REPLICATIONINBCELLLYMPHOMA
V.Tadi,P.BaiPalkovi,B.Sasi,M.Klasi,P.Kora
DivisionofMolecularBiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(petra.korac@biol.pmf.hr)
Mutations of specific genes are considered as key steps in tumorigenesis, but this process
equally includes transcription regulation and tumor cell adaptation to the signals from
microenvironment.AlthoughtumorcellsofspecificBcelllymphomahaveorigininBcellsfrom
thesamedifferentiationstage,theiradaptationtothemicroenvironmentchangesequilibrium
of proteins involved in replication process and makes them different from each other.
Replication then becomes less accurate leading to the accumulation of aberrations in DNA.
Additionalchangescontributetotumorprogression.Inthisstudyweanalyzedexpressionlevel
of prereplication/replication complex proteins (PCNA, MCM2, MCM7, GMNN and CDT1) and
proteins included in reassembly of chromatin after the replication (p300, DNMT1 and EZH2).
StudywasconductedontissuesampleswiththediagnosisofdifferentBlymphomas.Fromthe
tissue samples, using laser microdissection technique, tumor cells and nontumor parts were
isolated.ExpressionlevelswereanalyzedusingrealtimequantitativePCR.Asacontrol,tonsils
wereused.ResultsshowedsignificantDNMT1,MCM2,CDT1andp300overexpressionaswell
as geminin underexpression during Blymphoma progression. This study suggests importance
ofchangesinexpressionlevelsofgenesinvolvedinreplicationandchromatinreassemblyfor
malignanttumorprogression.
Keywords:Bcelllymphoma,replication,chromatin
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O120
RAZVOJTESTOVAZAANALIZUMETILACIJEDNAREGULATORNIHREGIJAGENAGMNNIEZH2I
NJIHOVA PRIMJENA U ISTRAIVANJU PODTIPOVA DIFUZNOG BVELIKOSTANINOGA
LIMFOMA
V.Vukovi*,M.Acman*,M.Klasi,P.Dobrini,P.Kora(*podjednakopridonijeliradu)
Zavodzamolekularnubiologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuilite
uZagrebu,Zagreb,Hrvatska(vvukovic@stud.biol.pmf.hr)
Kljunerijei:GMNN,EZH2,DLBCL,pirosekvenciranje,metilacijaDNA
V.Vukovi*,M.Acman*,M.Klasi,P.Dobrini,P.Kora(*equallycontributed)
DivisionofMolecularBiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(vvukovic@stud.biol.pmf.hr)
Keywords:GMNN,EZH2,DLBCL,pyrosequencing,DNAmethylation
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O121
HISTOKOMPATIBILNOST I IMUNOGENETIKA U TRANSPLANTACIJI SOLIDNIH ORGANA U
HRVATSKOJ
R.unec
Zavodzatipizacijutkiva,KBCZagreb,Zagreb,Hrvatska(rzunec@kbczagreb.hr)
ProgramtransplantacijesolidnihorganaodvijaseuHrvatskojod2007.g.uokvirumeunarodne
organizacije Eurotransplant. Pristupanje je uz ispunjavanje mnogobrojnih zakonskih i
organizacijskih uvjeta, zahtijevalo i posjedovanje laboratorija za tipizaciju tkiva s vaeom
akreditacijom Europske federacije za imunogenetiku (EFI) kako bi se osigurala standardizirana
kvaliteta rada na podruju histokompatibilnosti i imunogenetike. Ovaj preduvjet Hrvatska je
ispunilaakreditiranjemZavodazatipizacijutkivaKBCZagreb(ZZTT)2007.g.,kojijetimepostao
prvi medicinski laboratorij u Hrvatskoj s europskom akreditacijom. U ZZTT danas se provodi
imunogenetska obrada bolesnika za prijavu na listu ekanja za 4 transplantacijska centra (KBC
Zagreb, KB Merkur, KB Dubrava i KBC Osijek) te imunogenetska obrada kadaverinih davatelja
organa 15 donorskih bolnica. U razdoblju 2007.2015. godine imunogenetska obrada za listu
ekanjanapravljenajeza1842primateljabubrega,364primateljasrca,1074primateljajetrei92
primatelja guterae. U istom razdoblju provedeni su imunogenetski testovi za 1423
transplantacije bubrega, 230 transplantacija srca, 774 transplantacija jetre i 75 transplantacija
guterae.OvakointenzivanprogramuiniojeHrvatskujednomodvodeihzemaljausvijetuna
podruju transplantacije organa, emu Zavod za tipizaciju tkiva KBC Zagreb daje svoj struan i
znanstvenidoprinoskaoeuropskiakreditiranimunogenetskicentarizvrsnosti.
Kljunerijei:imunogenetika,tipizacijatkiva,transplantacija,solidniorgani
R.unec
TissueTypingCenter,UHCZagreb,Zagreb,Croatia(rzunec@kbczagreb.hr)
TransplantationprogramofsolidorgansinCroatiaistheintegralpartofEurotransplantsince
2007,whenCroatiabecomeafullmemberofthisbiggestEuropeannonprofitorganizationfor
optimal use and crossborder sharing of cadaveric donor organs. Joining Eurotransplant
requests fulfilling numerous organizational and legislative prerequisites having an EFI
accreditedtissuetypinglaboratorybeingtheoneofthem.Croatiafulfilledthesecriteriawhen
Tissue Typing Centre UHC Zagreb obtained the EFI accreditation in 2007, becoming the first
Croatian medical laboratory with European accreditation. Since then, Tissue Typing Center
Zagreb is responsible for immunogenetic testing of patients from 4 transplantation centers
(UHC Zagreb, CH Merkur, CH Dubrava and UHC Osijek) and for donor typing from 15 donor
hospitals. In the period 20072015 immunogenetic testing were conducted for 1842 kidney
recipients,364heartrecipients,1074liverrecipientsand92pancreasrecipients.Inthesame
period, testings were done for supporting 1423 kidney transplantations, 230 hear
transplantation,774livertransplantationsand75pancreastransplantations.Suchanintensive
donor and transplantation program put Croatia among the world most successful countries
withTissueTypingCentergivingitsprofessionalandscientificimpactasaEuropeanaccredited
immunogeneticcenterofexcellence.
Keywords:immunogenetics,tissuetyping,transplantation,solidorgans
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O122
HISTOKOMPATIBILNOST I IMUNOGENETIKA U ALOGENOJ TRANSPLANTACIJI KRVOTVORNIH
MATINIHSTANICAUHRVATSKOJ
Z.Grubi
Zavodzatipizacijutkiva,KBCZagreb,Zagreb,Hrvatska(zgrubic@kbczagreb.hr)
Kljunerijei:imunogenetika,tipizacijatkiva,transplantacija,krvotvornematinestanice
Z.Grubi
TissueTypingCenter,UHCZagreb,Zagreb,Croatia(zgrubic@kbczagreb.hr)
Hematopoieticstemcelltransplantation(HSCT)programinCroatiastartedin1983whenfirst
allogeneic transplantation from related donor was performed. Recruitment of the donor
following immunogenetic criteria is a crucial step in HSCT which is done exclusively in Tissue
TypingCenterUHCZagreb.RelatedHSCTprogramincludestestingsofaround150patientsand
700 family members annually, resulting in successful search of HLA genotipically identical
donor in 30% of families. Patients for whom the donor was not found within family are
included in HSCT program from HLA phenotipically identical unrelated donor. This program
reliesonnationalregistriesofunrelatedvolunteerHSCTdonorswhichareallincludedinthe
worldregistryBonemarrowdonorsworldwide(BMDW).Croatianbonemarrowdonorregistry
(CBMDR)hasmorethan40000registereddonors,allofthembeingHLAtypedinTissueTyping
Center Zagreb, while BMDW reached the number of 26 million donors. Protocols for finding
unrelated donor includes sophisticated search algorithms of CBMDR and BMDW, high
resolutionHLAtypingatfiveloci(A,B,C,DRB1,DQB1)andfinalconfirmatorytestingsofthe
recipientandthemostphenotipicallycompatibledonor.Around100patientsareincludedin
thisprogramannuallytogetherwithmorethan400potentialunrelateddonors.HSCTisalarge
and successful program in Croatia with a Tissue Typing Center UHC Zagreb being its integral
partwithacrucialrole.
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Keywords:immunogenetics,tissuetyping,transplantation,hematopoieticstemcells
O123
AKUTNIUTJECAJKOMBINIRANOGTRETMANADIJAFRAGMALNOGDISANJAIPROGRESIVNE
MIINE RELAKSACIJE NA ANTIOKSIDACIJSKU UINKOVITOST SLINE U MLADIH ODRASLIH
LJUDIPOVEZANSNJIHOVIMCRTAMAOSOBNOSTI
H.Lepedu1,A.Kurtovi1,I.Jozi2,D.GaoSoka3,I.Marinko1,V.Cesar4
1
Odsjek za psihologiju, Filozofski fakultet, Sveuilite J. J. Strossmayera u Osijeku, Osijek,
Hrvatska(hlepedus@yahoo.com)
2
Spreanska31,Osijek,Hrvatska
3
Prehrambenotehnolokifakultet,SveuiliteJ.J.StrossmayerauOsijeku,Osijek,Hrvatska
4
Odjelzabiologiju,SveuiliteJ.J.StrossmayerauOsijeku,Osijek,Hrvatska
Stres je postao sastavni dio modernog stila ivota dananjice, te sukladno tome i predmet
znanstvenih istraivanja u razliitim disciplinama. Brojni dokazi biokemijskih mehanizama
patogeneze velikog broja bolesti i poremeaja povezanih sa stresom pokazali su poveznicu sa
stvaranjem i djelovanjem reaktivnih kisikovih jedinki (ROS). Kad su prisutne u suviku, ROS
uzrokujuoksidacijskaoteenjanajvanijihbiomolekulatenatajnaindovodedoporemeajau
funkcioniranju stanica. Veliki broj molekula pokazuje antioksidacijska svojstva to omoguuje
uinkovitu zatitu od oksidacijskog stresa. U ovom istraivanju odreena je antioksidacijska
uinkovitost sline (SAC) mladih odraslih ljudi prije i neposredno nakon jednog kombiniranog
tretmana(utrajanjuodoko35min.)kojisesastojaooddijafragmalnogdisanja(DB)iprogresivne
miine relaksacije (PMR). I DB i PMR su nemedikamentozni tretmani koji se rutinski koriste u
klinikoj psihologiji i psihijatriji. Primjenom jednog tretmana dolo je do znaajnog poveanja
vrijednostiSAC.IndividualnerazlikeuporastuSACsu,meutim,bileurasponuod23%do60%.
Pokazalosadajetakavvelikirasapvrijednostiuznaajnojnegativnojkorelacijisneuroticizmom,
jednom od "velikih pet" crta osobnosti. Bioloki uinci koji su se pokazali u ovom istraivanju
potvrujupotrebuupotrebeDBiPMRkaovrlomonihtehnikanesamouklinikojpraksiveiu
smislusvakodnevnementalnehigijenekodzdravepopulacijemladihodraslihljudi.
H.Lepedu1,A.Kurtovi1,I.Jozi2,D.GaoSoka3,I.Marinko1,V.Cesar4
1
Department of Psychology, Faculty of Humanities and Social Sciences, University of J. J.
StrossmayerinOsijek,Osijek,Croatia(hlepedus@yahoo.com)
2
Spreanska31,Osijek,Croatia
3
FacultyofFoodTechnology,UniversityJ.J.StrossmayerinOsijek,Osijek,Croatia
4
DepartmentofBiology,UniversityofJ.J.StrossmayerinOsijek,Osijek,Croatia
Presently,stressbecameintegralpartofthemodernlifestyleandthusascientifictopicin
different disciplines. Biochemical mechanisms of stressrelated pathogenesis are well
documentedinabroadspectrumofillnessesanddisordersandwereshowntocomprisethe
formation and action of different reactive oxygen species (ROS). When present in excess,
ROS induce oxidative damage to essential biomolecules and in turn disturb normal cell
functioning. A plethora of molecules reveals antioxidative properties facilitating efficient
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protectionagainstoxidativestress.Inthisinvestigationsalivaantioxidantcapacity(SAC)was
measured in young adults before and immediately after a single combined treatment
(durationofapp.35min.)consistingofdiaphragmaticbreathing(DB)andprogressivemuscle
relaxation(PMR).Both,DBandPMRareroutinelyusedasnonmedicamentoustreatmentsin
clinicalpsychologyandpsychiatry.Afterasingletreatmentappliedthesignificantincreasein
SAC was revealed. However, the individual differences in SAC increase appeared to be
dissipatedfrom23to60%.Suchgreatdissipationwasfurtherconfirmedtobesignificantly
negatively correlated with the neuroticism, the one of the "big five" personality traits.
Biological effects revealed in our study confirmed the requirement of DB and PMR
applicationaspowerfulantistresstoolsinbothclinicalpraxisandespeciallyasdailymental
hygieneinhealthyyoungadultpopulation.
Key words: stress, saliva, diaphragmatic breathing, progressive muscle relaxation, non
medicamentousantistresstreatments
O124
MONKEYSWITHPERSONALITY:CONSISTENTINTERINDIVIDUALDIFFERENCESINCOMMON
MARMOSETS(Callithrixjacchus)
V.lipogor1,2,T.GunholddeOliveira1,J.J.M.Massen1,Z.Tadi2,T.Bugnyar1
1
Department of Cognitive Biology, University of Vienna, Vienna, Austria
(vedrana.slipogor@univie.ac.at)
2
Division of Animal Physiology, Department of Biology, Faculty of Science, University of
Zagreb,Zagreb,Croatia
Thefirstideasofpersonalitycamefromhumanpsychology,nowadaysisthisresearchquestion
studied in a wide range of study species, from insects to nonhuman primates. Consistent
individualdifferences(animalpersonalities)aredefinedasgroupsofcorrelatedbehaviours,that
aretemporallyandcontextuallyrepeatablewithinanindividual,andareusuallydividedinthree
nonsocial ("BoldnessShyness", "ExplorationAvoidance" and "Activity") and two social
("Aggressiveness"and"Sociability")behaviouraltraits.Inthisstudy,wetested21individualsfrom
three family groups of common marmosets (Callithrix jacchus) in five different experiments
(generalactivity,novelfood,novelobject,predatorandforagingunderrisk)inasolitarysetting.
Our results indicated that monkeys behaved consistently not only throughout time, but also
across different contexts. These behaviours formed a construct of four major nonsocial
personality components: "BoldnessShyness in Foraging", "BoldnessShyness in Predation",
"StressActivity"and"ExplorationAvoidance".Themodelsrevealednosexoragedifferencesin
thecomponents,buttheyrevealedadifferencebetweengroupsin"ExplorationAvoidance".As
common marmosets are highly gregarious animals, we will discuss the presence of the group
specificpersonalitytrait"ExplorationAvoidance"withregardstothepossibleinfluenceofsocial
environmentonbehaviourofindividuals,evenoutsidetheimmediatesocialcontext.
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O125
USPOREDBA MASNOKISELINSKOG SASTAVA POTKONOG MASNOG TKIVA SMEEG
MEDVJEDA(Ursusarctos)ISIVOGVUKA(Canislupus)
L.Vrankovi1,I.Dela2,S.Relji3,.Huber3,J.Kusak3,K.Klobuar4,G.Krivi4,D.Octenjak4,N.
Mikinac4,Z.Stojevi1,J.Aladrovi1
1
Zavod za fiziologiju i radiobiologiju Veterinarskog fakulteta Sveuilita u Zagrebu, Zagreb,
Hrvatska(lana.vrankovic@vef.hr,stojevic@vef.hr,jasna.aladrovic@vef.hr)
2
ZavodzakemijuibiokemijuMedicinskogfakultetaSveuilitauZagrebu,Zagreb,Hrvatska
3
ZavodzabiologijuVeterinarskogfakultetaSveuilitauZagrebu,Zagreb,Hrvatska
4
StudentiVeterinarskogfakultetaSveuilitauZagrebu,Zagreb,Hrvatska
Analiza masnokiselinskog sastava (MS) masnog tkiva koristi se za utvrivanje prehrambenih
navika slobodno ivuih ivotinja. Ovisno o vrsti, mnoge masne kiseline (MK) masnog tkiva
potjeu direktno iz hrane. Cilj ovog rada bio je bolje razumijevanje prehrambenih navika i
fiziolokogstatusaslobodnoivuihjedinkismeegmedvjeda(SM)isivogvuka(SV),tojekljuno
za strategije gospodarenja i zatite populacija divljih ivotinja. Istraivanje je provedeno na 91
jedinkiSM(enke:n=25,4,662,65g.;mujaci:n=66,5,302,72g.)i20jedinkiSV(enke:n=11,
1,951,86g.;mujaci:n=9,2,591,59g.).Uzorcisuskupljenitijekomsezonelova(uzorciSM2013.
i uzorci SV 2009.2011.). Nakon odmrzavanja uzoraka, homogenizacije i ekstrakcije lipida, MS
odreenjeplinskomkromatografijom.UMSpotkonogmasnogtkivaobjevrstedominirajuMKs
jednomdvostrukomvezom(48%uenkii41%umujakaSMvs.46%uenkii46%umujakaSV).
Zasiene MK ine 26% u enki i 38% u mujaka SM vs. 42% u enki i 44% u mujaka SV.
ViestrukonezasieneMK(PUFA)ine26%uenkii21%umujakaSM.UenkiSVPUFAsubile
zastupljeneu12%doksuumujakainile10%.UMSpotkonogmasnogtkivaSMiSVutvrene
suznaajnerazlikesobziromnadob,tjelesnumasu,sezonuilokacijuuzorkovanja.SlinostiMS
potkonogmasnogtkivauSMiSVproistjeuizfilogenezezvijeri,doksurazlikerezultatrazliitog
fiziolokogstatusaiprehrambenihnavikajedinki.
Kljunerijei:smeimedvjed,sivivuk,masnokiselinskisastav,potkonomasnotkivo
COMPARISONOFFATTYACIDCOMPOSITIONOFSUBCUTANEOUSADIPOSETISSUEOFBROWN
BEAR(Ursusarctos)ANDGRAYWOLF(Canislupus)
Analysisoffattyacid(FA)compositionisatechniqueusedtoexaminetheforaginghabitsoffree
ranging animals. Depending on the species, many of the FAs contained in adipose tissue are
derived directly from diet. The objective of this study was better understanding of foraging
patternsandphysiologicalstatusoffreerangingbrownbears(BB)andgraywolves(GW),whichis
essential for management and conservation strategies of wildlife populations. The study was
conductedon91BB(female:n=25,4.662.65yr.;male:n=66,5.302.72yr.)and20GW(female:
n=11,1.951.86yr.;male:n=9,2.591.59yr.).Sampleswerecollectedduringthelegalhunting
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season(2013forBBand20092011forGW).Afterthawing,homogenizationandlipidextraction,
the FA composition was determined by gas chromatography. The subcutaneous adipose tissue
(SAT)ofbothspeciesisdominatedbyFAswithonedoublebond(48%infemaleand41%inmale
BBvs.46%infemaleand46%inmaleGW).Saturatedfattyacidsconstitutedof26%infemale
and38% inmaleBBvs.42% infemale and 44%in maleGW. Polyunsaturatedfattyacidswere
foundin26%infemaleand21%inmaleBB.InfemalesGWPUFAwasin12%whileinmalesit
was10%.TheFAsofSATofBBandGWshowedage,bodymass,seasonandlocationrelated
significantdifferences.SimilaritiesinFAsofBBandGWarederivedfromphylogenyofcarnivores,
whilediferencesaretheresultofdifferentphysiologicalstatusandforagingpatterns.
Key words: brown bear, gray wolf, fatty acid composition, subcutaneous adipose tissue
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POSTERSKEPREZENTACIJE
POSTERS
KONZERVACIJSKABIOLOGIJA,ZATITAPRIRODEIOKOLIA
CONSERVATIONALBIOLOGY,NATUREANDENVIRONMENTPROTECTION
P1
KOCKAVICA (Fritillaria meleagris L., LILIACEAE) 10 GODINA AKCIJE PRAENJA STANJA ZA
OSNOVNEISREDNJEKOLE
I.Bori,V.PosavecVukeli,T.Kirin
Odjelzadivljeiudomaenesvojteistanita,Dravnizavodzazatituprirode,Zagreb,Hrvatska
(igor.borsic@dzzp.hr)
Kockavica (Fritillaria meleagris L., Liliaceae) je proljetnica vlanih livada i svijetlih, otvorenih
vlanih ikara i uma. Rasprostranjena je u kontinentalnoj Hrvatskoj, s nekoliko izoliranih
nalazita s podruja Velebita. Prema Crvenoj knjizi vaskularne flore Hrvatske smatra se
osjetljivomvrstom(VU).
Akcijapraenjastanjakockavicezapoelaje2005.godineusuradnjisakolamaMeimurskei
Krapinskozagorske upanije. Do danas se akcija proirila, te se provodi u suradnji s javnim
ustanovama za upravljanje zatienim prirodnih vrijednosti ukupno devet upanija,
Meimurske,Krapinskozagorske,Koprivnikokrievake,Varadinske,Bjelovarskobilogorske,
Sisakomoslavake, Zagrebake, Karlovake i Poekoslavonske upanije, te osnovnim i
srednjim kolama ovih upanija. Akcijom su prikupljeni znaajni podaci o rasprostranjenosti
kockaviceuHrvatskojkojisuprikazaniuovompriopenju.Istotako,akcijomjeutvrenokako
je kockavica s pojedinih nalazita nestala, uglavnom zbog melioracija, irenja graevinskih
podruja,izgradnjecesta tepretvaranja livadau obradivepovrine.Temeljemsvihdostupnih
podataka,kojiukljuujuliteraturnenavode,vlastitaterenskaopaanjatepodatkeprikupljene
akcijom praenja stanja kockavice provedena je revizija kategorije ugroenosti kockavice u
Hrvatskoj.
Kljunerijei:pravakockavica,prebrojavanje,kategorijaugroenosti,prijetnje
I.Bori,V.PosavecVukeli,T.Kirin
Department for Wild and Domesticated Taxa and Habitats, State Institute for Nature
Protection,Zagreb,Croatia(igor.borsic@dzzp.hr)
Snake'sheadfritillary(FritillariameleagrisL.,Liliaceae)isaspringflowerofwetgrasslandsand
light,openwetthicketsandforests.ItiswidespreadincontinentalCroatia,withafewisolated
localities from Velebit area. According to the Red Book of Vascular Flora of Croatia it is
considered a vulnerable species (VU). Snake's head fritillary monitoring campaign began in
2005 in cooperation with schools of Meimurje and KrapinaZagorje County. To date, the
actionspread,andisbeingconductedincollaborationwithpublicinstitutionsformanagement
of protected natural values of the nine counties, Meimurje, KrapinaZagorje, Koprivnica
Krievci, Varadin, BjelovarBilogora, SisakMoslavina, Zagreb, Karlovac and PoegaSlavonia
County, and primary and secondary schools of these counties. During the action important
dataonthedistributionofSnake'sheadfritillaryinCroatiawascollected,aspresentedinthis
presentation.Also,itwasfoundthattheSnake'sheadfritillaryhasdisappearedfromseveral
localities,mainlyduetolandameliorations,expansionofcommercialorresidentialareas,road
construction and the conversion of grassland to arable land. Based on all available data,
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including the literature data, our own field observations and data collected through the
monitoringcampaignthethreatcategoryofSnake'sheadfritillarywasreassessed.
Keywords:Fritillary,counting,threatcategory,threats
P2
DEFINIRANJE EKOLOKIH CILJEVA U SKLADU S EKOLOKOM MREOM NATURA 2000 ZA
POTREBEREVITALIZACIJEERDUTSKODALJSKOGDUNAVCA
A.ani1,Z.Mesi2,M.kunca1,A.Popija1,E.Patev1,S.Kapelj3,J.Krian4
1
Geonaturad.o.o.zastruneposlovezatiteprirode,Zagreb,Hrvatska(adanic@geonatura.hr)
2
Oikond.o.o.Institutzaprimijenjenuekologiju,Zagreb,Hrvatska
3
UdrugaBIOM,Zagreb,Hrvatska
4
Gekomd.o.o.,Hrvatska
PodrujeErdutskodaljskogdunavcajesustavodtririjenarukavcaudesnojinundacijiDunava.
Dio je ekoloke mree Natura 2000 (podruje ouvanja znaajno za ptice "HR1000016
Podunavlje i donje Podravlje", podruje ouvanja znaajno za vrste i stanita "HR2000372
DunavVukovar").Dosadizvedenigraevinskizahvati(eljeznikiicestovnimost,hidrotehniki
objekti za potrebe odravanja plovnog puta), uz prirodno zapunjavanje rijenim materijalom,
smanjenjedubineieutrofikaciju,doprinoseubrzanjusukcesijskihprocesa,zbogegadolazido
promjenestruktureifunkcijedunavca.Ciljprojektabiojeizraditiidejnorjeenjerevitalizacije
kojimbisedugoronoosiguraloizmjenuiosvjeavanjedunavcavodomizDunava,kakobise
postojei nepovoljni procesi usporili te ouvalo ili poboljalo stanje vodenih i movarnih
stanita, uz istovremeno osiguranje odrivog koritenja podruja. U sklopu projekta
revitalizacije utvreno je postojee stanje vodenih i movarnih stanita te znaaj podruja za
ouvanjefaune,snaglaskomnaciljnevrsteribaiptica.Analizomstanjaciljnihvrstaistanitate
kljunihekolokihuvjetapotrebnihzanjihovoouvanje,utvrenisuspecifini(ekoloki)ciljevi.
Svrha ekolokih ciljeva revitalizacije bila je dati smjernice i uvjete za projektiranje idejnog
rjeenja revitalizacije i planiranje koritenja prostora u svrhu rekreativnog ribolova i drugih
turistikihaktivnosti.
Kljune rijei: revitalizacija rijenih rukavaca, idejno rjeenje, Natura 2000, ouvanje ciljnih
vrstaistanita
A.ani1,Z.Mesi2,M.kunca1,A.Popija1,E.Patev1,S.Kapelj3,J.Krian4
1
GeonaturaLtd.ConsultancyinNatureProtection,Zagreb,Croatia(adanic@geonatura.hr)
2
OikonLtd.InstituteforAppliedEcology,Zagreb,Croatia
3
AssociationBIOM,Zagreb,Croatia
4
Gekomd.o.o.,Zagreb,Croatia
Theareaof"ErdutDaljdunavac"isthesystemofthreeriversidearmsintherightinundation
of Danube River. It is part of ecological network Natura 2000 (SPA "HR1000016 Podunavlje i
donjePodravlje",SCI"HR2000372DunavVukovar").Alreadyimplementedconstructionworks
(railway and road bridge, hydrotechnical objects for the maintenance of waterway), natural
filling with river sediments, lowering of depth and eutrophication, all together contribute to
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acceleration of succession processes, causing changes in structure and function of river side
arms. The project aim was to design concept solution of revitalization to ensure longterm
water exchange and refreshment with water from Danube main channel. This would slow
downtheexistentunfavourableprocessesinordertomaintainorimprovethestateofaquatic
and wetland habitats, and allow sustainable use of the area. Current state of aquatic and
wetland habitats was assessed, as well as the value of the project area for conservation of
fauna,withanemphasisontargetfishandtargetbirdspecies.Specific(ecological)objectives
weredefinedbasedontheanalysisofcurrentstatusoftargetspeciesandtargethabitats,and
keyecologicalrequirementsneededfortheirconservation.Thepurposeofdefiningecological
objectives of revitalization is to give recommendations and framework for designing the
conceptsolutionandplanning theuse oftheareaforrecreationalfishingandothertouristic
activities.
Keywords:riversidearmsrevitalization,conceptsolution,Natura2000,conservationoftarget
speciesandtargethabitats
P3
SUIVOTLJUDIIPRIRODENAPRIMJERUNACIONALNOGPARKAPLITVIKAJEZERAJELIISTO
MOGUE?
M.Gubi,M.Barievi,D.Fantina,A.Mojsoviui
Zdravstvenoveleuilite,Zagreb,Hrvatska(ana.mojsoviccuic@zvu.hr)
Ciljprovedenogistraivanjabiojeispitatikakvesumogunostisuivotastanovnitvazatienog
podruja s prirodom te jesu li u skladu sa zakonskom regulativom. U svrhu istraivanja
provedeno je anketiranje meu lokalnim stanovnitvom. Na temelju rezultata ankete te
direktnog uvida u nain ivota lokalnog stanovnitva u sklopu NP Plitvika jezera moe se
zakljuiti da lokalno stanovnitvo u najveoj mjeri i dalje ivi od turizma koji je vezan uz
postojanje Nacionalnog parka, no ne u tolikoj mjeri kao nekad. Kao najvei problem ivota u
ovom kraju stanovnici istiu da je kolovanje mladih, u svrhu daljnjeg zapoljavanja u parku
skoro u potpunosti nestalo, kako lokalne poljoprivrede povezane s opskrbom ugostiteljsko
turistikihsadrajauparkuvienema,asezonskeposloveradeljudiizsvihkrajevaHrvatske,
bezprednostilokalnogstanovnitva.SmatrajukakobiUpravaparka,kaoiopine,zatienom
podruju trebali pridati mnogo veu pozornost te bi samim time poboljali suivot prirode i
lokalnog stanovnitva. Na samom kraju namee se zakljuak kako je suivot prirode i
stanovnitva mogu ukoliko se postigne ravnotea izmeu potivanja zakonskih odredbi o
zatitiprirodeteukolikoisteneonemoguujuradnjenunezanormalanivot.
Kljunerijei:Nacionalnipark,Plitvikajezera,lokalnostanovnitvo,suivot,priroda
COEXISTENCEOFLOCALPOPULATIONANDNATUREINTHEEXAMPLEOFTHEPLITVICELAKES
NATIONALPARKISITPOSSIBLE?
M.Gubi,M.Barievi,D.Fantina,A.Mojsoviui
UniversityofAppliedHealthSciences,Zagreb,Croatia(ana.mojsoviccuic@zvu.hr)
Thegoalofresearchwastoexaminewhatarethepossibilitiesofcoexistenceofthepopulation
intheprotectedareawiththenatureandwhethertheyareincompliancewiththestatutory
regulations. For the purpose of research, an opinion poll was conducted among the local
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12.HBK14312 CBC
population.Basedontheresultsofthepollanddirectinspectionofthemanneroflifeofthe
localswithinthePlitviceLakesNationalPark,itmaybeconcludedthatthelocalpopulationfor
the most part still lives of tourism, which is related to the National Park, but not in the
proportions as it once did. As the major problem in their life, the locals point out that the
schooling of the young for the purpose of further employment in the park has almost
completely disappeared, that there is no longer local agriculture linked to the supply of the
cateringtouristfacilitiesinthepark,andthelocalsnolongerhaveprivilegesinseasonaljobs
whicharenowadaysalsoavailabletootherpeoplefromallpartsofCroatia.Theythinkthatthe
park'sManagement,aswellasthemunicipalities,shoulddevotemuchgreaterattentiontothe
protected area, which would simultaneously improve coexistence of the nature and local
population. At the end, the conclusion is that the coexistence of nature and locals is only
possible if the balance is achieved between the compliance of statutory provisions on
protectionofnatureandifsuchprovisionsdonothinderactivitiesrequiredfornormallife.
Keywords:NationalPark,PlitviceLakes,localpopulation,coexistence,nature
P4
ANALIZAMETODAZAPRAENJEPRIJELAZADIVLJIHIVOTINJAPREKOZELENIHMOSTOVA
G.Guvica1,I.Bonjak2,A.Bielen3,D.Babi4,B.RadanoviGuvica5,L.ver3
1
Grupazavelikezvijeri,Geonaturad.o.o.,Zagreb,Hrvatska(gguzvica@oikon.hr)
2
Sveuilite u Zagrebu, Prirodoslovnomatematiki fakultet, Botaniki zavod, Zagreb, Hrvatska
3
SveuiliteuZagrebu,Prehrambenobiotehnolokifakultet,Zavodzabiokemijskoinenjerstvo,
Zagreb,Hrvatska
4
ByteLab,Zagreb,Hrvatska
5
Hrvatskiprirodoslovnimuzej,Zagreb,Hrvatska
Zelenimostovismanjujunegativanuinakprometnicanapopulacijedivljihivotinja,anjihova
uinkovitost procjenjuje se razliitim metodama praenja. Temeljem trogodinjeg praenja
etiri zelena mosta na autocesti A1, usporeena je uinkovitost metode praenja tragova na
kontrolnoj pjeanoj traci, metode fotozamki i metode infracrvenih (IC) senzora. Za svaku
metodu je utvreno koliko tono detektira razliite vrste divljih ivotinja, kao i moe li se
temeljemnjeprocijenitistvarnibrojprijelaza.Granulometrijskisastavpjeanetrakeutjecaoje
na tonost odreivanja ivotinjskih vrsta. Najbolji rezultati dobiveni su kada je koriten
materijalsveimudjelomsiltaigline.tosetiesastavavrsta,metodompraenjatragovana
kontrolnojtracipotcijenjenjeudiomalihkanida,dokjemetodomfotozamkipotcijenjenudio
srna. Nadalje, metodom fotozamki je potcijenjen ukupan broj prijelaza ivotinja. Suprotno
tome,ICsenzorimajezabiljeenood11do19putavieprijelazanegofotozamkama.Meutim,
oko 80% IC dogaaja bili su artefakti, odnosno nisu ih uzrokovali prijelazi ivotinja. Stoga je
razvijen algoritam za korekciju IC podataka kako bi se dobila tonija procjena stvarnog broja
prijelaza. Zakljuno, prikazani rezultati predstavljaju vrijedan doprinos jer daju pregled
prednostiinedostatakarazliitihmetodamonitoringateedoprinijetiveojtonostirezultata
praenjaprijelazadivljihivotinjaprekozelenihmostova.
Kljunerijei:zelenimostovi,monitoringprijelazaivotinja,usporedbametoda
ANALYSISOFMETHODSFORWILDLIFEMONITORINGONGREENBRIDGES
G.Guvica1,I.Bonjak2,A.Bielen3,D.Babi4,B.RadanoviGuvica5,L.ver3
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12.HBK14412 CBC
Groupforlargecarnivores,GeonaturaLtd.,Zagreb,Croatia(gguzvica@oikon.hr)
UniversityofZagreb,FacultyofScience,DepartmentofBotany,Zagreb,Croatia
3
University of Zagreb, Faculty of Food Technology and Biotechnology, Department of
BiochemicalEngineering,Zagreb,Croatia
4
ByteLab,Zagreb,Croatia
5
CroatianNaturalHistoryMuseum,Zagreb,Croatia
Keywords:greenbridges,wildlifecrossingmonitoring,comparisonofmethods
P5
PRELIMINARNIREZULTATIISTRAIVANJAVUKOVAUPARKUPRIRODEBIOKOVO
G.Guvica1,2,I.Gabri3,K.Protrka3,Z.Grguri1,L.ver4
1
Grupa za velike zvijeri, Geonatura d.o.o., Zagreb, Hrvatska (gguzvica@geonatura.hr,
gguzvica@oikon.hr)
2
Zavod za kopnene ekosustave, Oikon d.o.o., Institut za primijenjenu ekologiju, Zagreb,
Hrvatska3Javnaustanova"ParkprirodeBiokovo",Makarska,Hrvatska
4
Zavodzabiokemijskoinenjerstvo,Prehrambenobiotehnolokifakultet,SveuiliteuZagrebu,
Zagreb,Hrvatska
Uokviruprojekta"IstraivanjeimonitoringvukovauParkuprirodeBiokovo"od2013.godine
provodiseistraivanjesciljemutvrivanjaprisutnosti,brojnostiistabilnostioporavukovate
utvrivanja veliine podruja obitavanja opora. U istraivanju se primjenjuju metode
fotozamki, praenja tragova, akustina metoda i metoda GPS telemetrije te analiza strukture
prehrane.Tijekomprvih18mjeseciistraivanjapostavljenesufotozamkena20lokacijatesu
prikupljene 3922fotografijepogodne za analizu. Vuk je snimljen na 5 razliitih lokacija na46
fotografijaodnosno1,17%slika.Naistojfotografijisuzabiljeenenajviedvijejedinkevuka.Na
temeljutragovailiizmetaprisutnostvukautvrenajena25lokacijatejeprikupljeno53izmeta
pogodnih za analizu strukture prehrane. Provedbom akustine metode odaziv vukova
zabiljeen je na osam lokacija. Samo na jednoj lokaciji utvren je odaziv najmanje 5 jedinki.
Provedba GPS telemetrije je u fazi hvatanja i obiljeavanja jedinke vuka. Na temelju
dosadanjih rezultata jo nije mogue izvesti pouzdane zakljuke o broju opora i brojnosti
2
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jedinki vuka u Parku prirode Biokovo. Rezultati akustine metode ukazuju na najmanje pet
jedinki u oporu, dok ostale metode ukazuju na pojedinano pojavljivanje vukova kao
privremeno ili trajno dispergiranih jedinki ili ostatku "razbijenog" opora to je mogue
posljedicaznaajnogpritiskanavukaodstranelovacaistoara.
Kljunerijei:sivivuk,Canislupus,ParkprirodeBiokovo
THERESEARCHOFWOLVESINTHENATUREPARKBIOKOVOPRELIMINARYRESULTS
G.Guvica1,2,I.Gabri3,K.Protrka3,Z.Grguri1,L.ver4
1
Group for large carnivores, Geonatura Ltd., Zagreb, Croatia (gguzvica@geonatura.hr,
gguzvica@oikon.hr)
2
Department for terrestrial ecology, Oikon Ltd., Institute for Applied Ecology, Zagreb, Croatia
3
PublicInstitution"NatureParkBiokovo",Makarska,Croatia
4
Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology,
UniversityofZagreb,Zagreb,Croatia
From2013,theproject"ResearchandmonitoringofwolvesintheNatureParkBiokovo"isbeing
conducted to determine the presence, abundance and stability of the wolf packs and to
determine thesize oftheirhome range. Monitoring methodsbeing applied are: digitalcamera
traps,recordingoffootprintsandscat,acousticmethod,GPStelemetryandanalysisofthediet.
Duringthefirst18monthsofresearch,cameratrapswereplacedat20locationsand3922photos
suitable for analysis were collected. Wolves were recorded in 46 photos (1.17%) at 5 different
locations. No more than two wolves were recorded in the same photograph. According to
observedfootprintsorscats,thepresenceofwolfwasdeterminedat25locationsand53scats
suitablefordietanalysiswerecollected.Usingacousticmethod,howlingreplywasrecordedat8
locationswhilethereplyofatleastfiveindividualsisrecordedonlyinonelocation.GPStelemetry
isatthestageofcapturingandmarkinganindividual.Basedonthepreliminaryresultsitisnot
possibletodrawreliableconclusionsaboutthenumberofpacksandnumberofwolfsindividuals
intheNatureParkBiokovo.Resultsofacousticmethodindicateatleastfiveindividualsinapack,
whileothermethodspointtoappearanceofindividualsasatemporaryorpermanentdispersed
individuals or the remainder of the "broken" pack which could be the result of a significant
influenceonthewolfbyhuntersandstockbreeders.
Keywords:greywolf,Canislupus,NatureParkBiokovo
P6
NOVA STRANA VRSTA DESETERONONIH RAKOVA (MRAMORNI RAK (Procambarus fallax
(Hagen,1870)f.virginalis)USLATKOVODNIMEKOSUSTAVIMAHRVATSKE
S.Hudina1,M.Samardi2,A.Luci1,M.Jeli1,I.Maguire1
1
Zoologijskizavod,Prirodoslovnomatematikifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
(misel.jelic@biol.pmf.hr)
2
Gimnazija"FranGalovi",Koprivnica,Hrvatska
Mramorni rak (Procambarus fallax (Hagen, 1870) f. virginalis) trea je alohtona vrsta
slatkovodnih deseterononih rakova koja zabiljeena u vodotocima Hrvatske. Ova
partenogenetskavrstarakazabiljeenajeprviput2013.godineuljunariodericanasjeveru
Hrvatske. Tijekom 2015. godine ispitali smo potencijalne putove unosa mramornog raka u
Hrvatsku, te istraili osnovne znaajke populacije zabiljeene u ljunari oderica (relativna
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gustoa rakova, uzrasna struktura populacije, znaajke godinjeg ciklusa) i potencijal daljnjeg
irenjaovevrstenapodrujuMeimurjaiPodravine.Kakobiupravljanjeiaktivnostiusmjerene
na kontrolu irenja ove vrste bile pravovremene, neophodni su detaljni podaci o strukturi i
dinamicipopulacijemramornograkauoderici.
S.Hudina1,M.Samardi2,A.Luci1,M.Jeli1,I.Maguire1
1
Division of Zoology, Faculty of Science, University of Zagreb, Zagreb, Croatia
(misel.jelic@biol.pmf.hr)
2
GymnasiumFranGalovi,Koprivnica,Croatia
Themarblecrayfish(Procambarusfallax(Hagen,1870)f.virginalis)isthethirdnonindigenous
crayfishspeciesestablishedinCroatianfreshwaterhabitats.Thisparthenogeneticspecieswas
discoveredin2013intheodericagravelpitlake,locatedinthenorthofCroatia.Inthisstudy
we looked into potential introduction pathways of the marble crayfish to Croatia, examined
some of its basic population characteristics (i.e. relative crayfish abundance, population size
structureandyearcyclecharacteristics)andanalyseditspotentialfordispersalintheregion.
Research and monitoring programme for the oderica marble crayfish population is urgently
requiredinordertoimmediatelystartwithmanagementactionstargetedatitscontrol.
P7
SMJERNICEZAPROCJENUUTJECAJAVJETROELEKTRANANAVELIKEZVIJERI
J.Kusak1,.Huber1,N.Trenc2,J.Jeremi2,S.Desnica2
1
Zavodzabiologiju,VeterinarskifakultetSveuiliteuZagrebu,Zagreb
2
Dravnizavodzazatituprirode,Zagreb(sonja.desnica@dzzp.hr)
12.HBK14712 CBC
Kljunerijei:vjetroelektrane,velikezvijeri,ocjenautjecaja,smjernice,GIS
GUIDELINESFORASSESSMENTOFWINDFARMSIMPACTONLARGECARNIVORES
J.Kusak1,.Huber1,N.Trenc2,J.Jeremi2,S.Desnica2
1
BiologyDepartmentofVeterinaryFaculty,UniversityofZagreb,Zagreb
2
StateInstituteforNatureProtection,Zagreb(sonja.desnica@dzzp.hr)
The Dinaric mountain range due to its wind potential is the preferred area for wind farm
development in Croatia but in the same time it represents a valuable habitat for large
carnivores (wolf, bear and lynx). The biggest threat to large carnivores survival is habitat
fragmentationandloss.Windfarmswithaccessroadsandassociatedisturbancesignificantly
contributetothatprocess.Toensureadequateassessmentofwindfarmimpactsindifferent
environmental and nature protection procedures pertaining to national and EU legislation,
Biology Department of Veterinary Faculty in Zagreb and State Institute for Nature Protection
jointly developed Guidelines for assessment of wind farms impact on large carnivores. The
experience shows that the best approach for reduction and avoidance of individual and
cumulative impacts of wind farms is their appropriate spatial p ositioning. Therefore the
Guidelines propose an assessment methodology based on the sensitivity map, which was
created throughout mathematical modeling and GIS using data on large carnivores
occurrences and related habitat characteristics. The sensitivity map indicates importance of
certain area for each species of large carnivores and defines nine categories of significance.
Also percent and absolute limits of further loss for each category have been determined. In
addition,theappropriateuseofsensitivitymapinasassessmentproceduresisdescribedand
proposed.
Keywords:windmills,largecarnivores,impactassessment,guidelines,GIS
P8
MODIFICIRANILESLIELEFKOVITCHGENETIKIALGORITAMSKIMODELSKISUSTAVDINAMIKE
POPULACIJAKOMARACA
.Lonari,M.Jambrovi,B.K.Hackenberger
Zavodzakvantitativnuekologiju,OdjelzabiologijuSveulitaJosipaJurjaStrossmayera,Osijek,
Hrvatska(jambrovic.martina@gmail.com)
Fluktuacijeveliineipromjenestarosnestrukturepopulacijakomaracatijekomgodinevanisu
parametri nuni za planiranje i provoenje mjera kontrole brojnosti komaraca na nekom
podruju.Osimtoga,dinamikapopulacijakomaracajeiizuzetnozanimljivoakademskopitanje
zbognjenihosobinakaotosupodlonostAlleeefektuiperiodinakaotinost.Upravozbogtih
osobina je dinamiku populacija komaraca teko predviati temeljem modela nainjenih
uobiajenim tehnikama. Do sada su najbolje rezultate u modeliranju dinamike populacija
komaraca pokazali modificirani diskretni LeslieLefkovitch matrini modeli (MLL). Kako bi se
poboljala svojstva predikcije rezultata tretiranja komaraca larvicidima i adulticidima te
doprinijelo izradi raunalne podrke sustavu odluivanja, u ovom radu su se postojeim
modelimadodaliprogramskimodulinatemeljugenetikihalgoritama.Takodobivenihibridni
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sustaviskoristiosezadobivanjeserijapredikacijakojesuusporeenespredikcijamadobivenim
pomou jednostavnog MLLsustava. Osim u poboljanju prediktivnosti rezultata tretiranja,
hibridni sustav je pokazao i nova aplikativna svojstva modela koja znatno doprinose
raunalnomupravljanjukontrolekomaraca.
Kljunerijei:komarci,genetikialgoritam,MLL,predikcija
.Lonari,M.Jambrovi,B.K.Hackenberger
Department of Biology, Josip Juraj Strossmayer University Osijek, Osijek, Croatia
(jambrovic.martina@gmail.com)
Fluctuationsinsizeandchangesinagestructureofmosquitopopulationsduringtheyearare
important parameters necessary for planning and implementation of mosquito control
measures.Additionally,mosquitopopulationdynamicisanexceptionallyinterestingacademic
question due to its periodic chaos system and susceptibility to Alleeeffect. It is those
characteristics that make mosquito population dynamic difficult to predict using the usual
methods.Untilnow,discreteLeslieLefkovitchmatrixmodels(MLL)showedthebestresultsin
mosquitopopulationdynamicmodelling.Toimproveresultpredictionpropertiesoflarvicides
and adulticides, and to contribute to the development of computer support to the deciding
system,programmodulesbasedongeneticalgorithmswereaddedtotheexistingmodels.This
hybrid system was used to make a series of predictions whic h were compared to the
predictions made by simple MLLsystem. Hybrid system, aside from improving prediction of
treatment results, showed new applicable model properties which significantly contribute to
computermanagementofmosquitocontrol.
Keywords:mosquitoes,geneticalgorithm,MLL,predictions
P9
IMIINABRIJUNIMAREZIDENCIJALNAELITAILIMASOVNITURIZAM?
M.Mazija,Z.Domazetovi,B.Benina,A.tefan,.Drdar
UdrugazazatituimiaTragus,Zagreb,Hrvatska(abc.tragus@gmail.com)
PrikupljanjeprvihpodatakaofauniimiaNacionalnogparkaBrijunizapoelojepreliminarnim
istraivanjem u rujnu 2010. godine. Pretraen je vei broj potencijalnih sklonita i provedena
pregledna istraivanja hvatanjem mreama i snimanjem ultrazvunim detektorom. Ve prvi
podacipokazalisuiznenaujuubrojnostjedinkiiraznolikostvrsta.Iztograzlogasuurazdoblju
od2011.do2014.nastavljenaistraivanjanaveembrojuobjekatatenaodabranimstanitima
znaajnim za imie. Istraivanja su provedena na Velikom i Malom Brijunu, ali i na drugim
manjim otocima Brijunskog arhipelaga. Hvatanjem mreama, snimanjem ultrazvunim
detektorom i pretragom objekata utvreno je ukupno 14 vrsta imia. Znaajno je istaknuti
prisutnost porodiljne kolonije vrste Myotis nattereri, jedinke vrste Myotis mystacinus u
hibernaciji na otoku Mali Brijun te uhvaenog mujaka vrste Nyctalus lasiopterus s izraenim
testisimaibukalnimlijezdama,tojetekdruginalazivejedinkeovevrsteuHrvatskojnakon
89godina.Analizasonogramaukazujenapodrujasveombrojnouvrstaigustoomjedinki
tenaprostorekojekoristevrsteodosobitogznaaja.Temeljemanalizerezultataistraivanja,
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Kljunerijei:imii,NacionalniparkBrijuni
BATSONBRIJUNIRESIDENTIALELITEORMASSTOURISM?
M.Mazija,Z.Domazetovi,B.Benina,A.tefan,.Drdar
AssociationforBatConservationTragus,Zagreb,Croatia(abc.tragus@gmail.com)
Initial data gathering on bat fauna in Brijuni National Park began with preliminary research in
September2010.Manypotentialsiteswereexaminedandscreeningwasconductedbycatching
with mist nets and recording with ultrasound detector. The first data indicated a surprising
number of individuals and great species diversity. For this reason, during the period between
2011 and 2014, research was extended to a greater number of sites and selected habitats
important to bats. The research was conducted on Veliki and Mali Brijun, as well as on other
smaller islands within the Brijuni archipelago. Altogether, 14 bat species were found by mist
netting, recording with ultrasound detectors and examination of sites. It is important to
emphasise the presence of Myotis nattereri maternity colony, hibernating Myotis mystacinus
individualsonislandMaliBrijunandcapturedNyctaluslasiopterusmale,withenlargedtestesand
buccalglands,whichisthesecondfindingofaliveindividualofthisspeciesinCroatiaafter89
years.Sonogramanalysisrevealsareaswithgreaternumberofspeciesanddensityofindividuals,
aswellasareasusedbyspeciesofimportance.Basedontheanalysisoftheresults,measuresto
protect bats and support bat populations in Brijuni National Park were suggested. Given the
importance of the collected data, further research will be focused on monitoring of the key
elements(habitatsandspecies)intheperiodsimportantfortheannualcycleofbats.
Keywords:bats,BrijuniNationalPark
P10
RASPROSTRANJENOSTITRENDGNIJEZDEEPOPULACIJEVRANEGAAC(CorvusfrugilegusL.
1758)NAPODRUJUOSIJEKA
A.Mikuka1,A.Felja1,D.Bjedov2,K.Kajan2,M.Blaevi2,A.Zovko2,P.Beni2
1
Zavod za zoologiju,Odjel za biologiju, Sveuilite Josipa Jurja Strossmayera u Osijeku, Osijek
(amikuska@biologija.unios.hr)
2
UdrugastudenatabiologijaZOA,SveuiliteJosipaJurjaStrossmayerauOsijeku,Osijek
Iakojeunedavnojprolostibilatipinastepskaptica,vranagaac(Corvusfrugilegus)jedanas
svebrojnijauurbanimpodrujima.Ciljistraivanjajeutvrivanjetrendagnijezdeepopulacije,
te utvrivanje veliine zimujue populacije vrane gaca na podruju grada Osijeka.
Prebrojavanje broja gnijezdeih parova gaaca u kolonijama obavljeno je metodom brojanja
sigurno aktivnih gnijezda u kolonijama tijekom proljea 2014. i 2015. godine, a tijekom zima
gacisubrojaninaokupljalitimaprijeodlaskanaspavalite.Obradomliteraturnihipodatakas
terenskihistraivanjauzpomosoftverskogpaketaTRIMizraunatjetrendpopulacijeuzadnje
23 godine. Tijekom zima 2014. i 2015. godine, na okupljalitima se prije spavanja nalazilo
prosjeno 4000 gaaca. Tijekom 2014. godine na podruju grada Osijeka gnijezdilo je 560
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12.HBK15012 CBC
parovagaacau13kolonija,dokjeu2015.godinignijezdilo623parovau12kolonija.Najvei
brojkolonija(est)pripadamalimkolonijama,do50parova,ukojimajeprosjenognijezdilo19
parova.Srednjevelikihkolonija(50100parova)jebiloizmeujedne(2014.)ietiri(2015.),a
velikihkolonijasavieod100parovasutijekomistraivanogperiodajebilosamodvije.Trend
gnijezdee populacije gaaca na podruju grada Osijeka tijekom 23 godine je umjereno
opadajui.Glavnirazlozitomujesustalnarastjerivanjakolonija,orezivanjestabalanakojimasu
kolonijeizgraene,teruenjenjihovihgnijezda.
Kljunerijei:vranagaac,trendgnijezdeepopulacije,Osijek
DISTRIBUTIONANDTRENDOFBREEDINGPOPULATIONOFROOKS(CorvusfrugilegusL.1758)
INOSIJEK
A.Mikuka1,A.Felja1,D.Bjedov2,K.Kajan2,M.Blaevi2,A.Zovko2,P.Beni2
1
Subdepartmentofzoology,DepartmentofBiology,JosipJurajStrossmayerUniversityofOsijek,
Osijek,Croatia(amikuska@biologija.unios.hr)
2
BiologyStudentsAssociationZOA,DepartmentofBiology,JosipJurajStrossmayerUniversity
ofOsijek,Osijek
DespitethefactthatRookwasatypicalbirdofthesteppehabitats,theirnumbersarenowadays
increasinginurbanareas.Theaimofthisstudyistopresentthetrendofbreedingpopulationand
size of wintering population of Rook in Osijek town. Breeding population was established by
counting Apparently Occupies Nest in Rook colonies during springs. Wintering population was
studiedbycountingRookattheirroostsduring2014and2015.SoftwarepackageTRIMwasused
to calculate the breeding population trend for last 23 years. On winter roosting localities 4000
Rookswerecountedinaverageduringwintersof2014and2015.During2014560breedingpairs
werecountedin13colonies,while623breedingpairswerebreedingin12coloniesduring2015.
Majorityofthecolonies(6)wereclassifiedassmall(below50pairs),withaverageof19nesting
pairs.Numberofmediumsizedcolonies(50100pairs)variedbetweenonein2014andfourin
2015,whiletwobigcolonies(over100pairs)werepresentinbothyears.Inthepast23yearsthe
breeding population trend shows moderate decline, mainly because of the harassment of the
colonies,nestingsitesdestructionanddisturbanceofbreedingRooks.
Keywords:Rook,breedingpopulationtrend,Osijek
P11
OPTIMIZACIJAMETODAZAMOLEKULARNOODREIVANJESPOLAKODAPLJI(ARDEIDAE)
A.Mikuka,L.Begovi,I.Mihi,K.Kljaji,R.Grgac
Zavod za zoologiju,Odjel za biologiju, Sveuilite J. J. Strossmayer u Osijeku, Osijek, Hrvatska
(alma.mikuska@biologija.unios.hr)
Genetskimarkerisukoristanalatzapraenjepromjenaupopulacijskimstudijama.Ciljradaje
optimiziranjemetodezaizolacijuDNAiodreivanjespolamolekularnimmetodamakodaplji
(Ardeidae).GenomskaDNAjeizoliranaizperjailjuskijajakoristeidvarazliitaprotokola.Za
odreivanje spola koritene su poetnice 2550F/2781R za kromohelikazu DNAvezujui gen
(CHD). Rezultati su pokazali da je najvei prinos DNA dobiven iz pera koja su u razvoju
(prokrvljena pera) i ljuski jaja. Prinos DNA je bio manji kad se koristio komercijalni kit za
izolaciju u odnosu na izolaciju s TNE puferom uz dodatak natrijevog acetata za taloenje
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12.HBK15112 CBC
keratina.Kljuankorakuizolacijijekoncentracijaditiotreitola(DTT)iproteinazeKupuferuza
liziranjekaoikoliinakrviprisutnauperju.
Kljunerijei:genetskimarkeri,odreivanjespola,aplje
OPTIMIZATIONOFMETHODSFORMOLECULARSEXTYPINGINHERONS(ARDEIDAE)
A.Mikuka,L.Begovi,I.Mihi,K.Kljaji,R.Grgac
Subdepartment for Zoology, Department of Biology, Josip Juraj Strossmayer University of
Osijek,Osijek,Croatia(alma.mikuska@biologija.unios.hr)
Genetic markers are useful tool for tracking changes in population studies. This work, is
focused on optimization of method for DNA isolation and molecular sex determination in
herons (Ardeidae). Genomic DNA was isolated from feathers and eggshells by using two
different protocols. For sextyping of herons we used primers 2550F/2781R for chromo
helicaseDNAbindinggene1(CHD1).ResultsshowedthatthehighestDNAyieldwasobtained
fromthebloodfeathersandeggshellswhilematurefeathershadthelowestyield.DNAyield
was lower when commercial kit was used for isolation compared to the isolation with TNE
buffer followed by addition of sodium acetate for keratin precipitation. Critical step is
concentrationofdithiothreitol(DTT)andproteinaseKinlysissolutionaswellastheamountof
bloodpresentinthefeathers.
Keywords:geneticmarkers,sextyping,herons
P12
BIOLOKAZATITAPITOMOGKESTENAGLJIVAMAANTAGONISTIMA
S.NovakAgbaba1,I.Frani2
1
Zavodzazatituumailovnogospodarenje,Hrvatskiumarskiinstitut,Jastrebarsko,Hrvatska
(sanjan@sumins.hr)
2
Cuglini1,Zagreb,Hrvatska
Kljunerijei:pitomikesten,Cryphonectriaparasitica,gljiveantagonisti,biolokazatita
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12.HBK15212 CBC
BIOLOGICALCONTROLOFSWEETCHESTNUTWITHANTAGONISTICFUNGI
S.NovakAgbaba1,I.Frani2
1
Division for forest protection and game management, Croatian forest research institute,
Jastrebarsko,Croatia(sanjan@sumins.hr)
2
Cuglini1,HR10000Zagreb,Croatia
Chestnut stands are threatened by two invasive organisms, fungus Cryphonectria parasitica and
gallwaspDrycosmuskuriphilus.ThefungusC.parasiticacausecancerbarkdisease.GallwaspD.
kuriphiluscausethedamageonbudsandleavesandformgallsinthecrown.Onthegalls,branches
and nut C. parasitica, saprophytic and parasitic fungi were found. Apart from them antagonistic
fungi,usuallyTrichodermaviride,TrichodermacitrinovirideandTrichotheciumroseumwerefound.
TheaimofthisstudywasinvestigatetheeffectoftheantagonisticfungionC.parasiticagrowth
and possibility of biological control. To determine the interaction between antagonistic fungi T.
viride,T.citrinovirideandT.roseumandC.parasiticathelaboratorytestwereestablished.T.viride
hasmadecontactondays45,andwasovergrownbyC.parasiticawithin610days.T.citrinoviride
hasmadethecontactondays46,andwasovergrownorpartialovergrownwithin2546days.T.
roseumhasmadecontactonday911,andwasovergrownwithin152942days.T.viridehasthe
fastestgrowandthemostpowerfulantagonisticactivity.AntagonisticfungiT.viride,T.citrinoviride
and T. roseumare ubiquitous in chestnutstands.Laboratory experimentdemonstrated thatthe
antagonisticfungi were inhibitedC. parasitica. This indicates that in thechestnut standsnatural
biologicalcontrolwithantagonisticfungiispresent.
Keywords:sweetchestnut,Cryphonectriaparasitica,antagonisticfungi,biologicalcontrol
P13
SOLARNIPARKKOSOREUTJECAJINAOKOLIIEKOLOKUMREU
N.Pavlus,M.Draki,D.valjek,M.Peri,D.egvi,M.Vukovi,B.Matoi,S.Blaevi
Zeleniservisd.o.o.,Split,Hrvatska(mihael.draksic@zeleniservis.hr)
Solarni park Kosore graditi e se na podruju radne zone Kosore, na podruju grada Vrlike.
Planirana je gradnja sedam razliitih fotonaponskih elektrana na tlu, svaka snage 300 kW.
Zahvat e omoguiti proizvodnju elektrine energije, koja e se distribuirati u
elektroenergetskumreu.Obziromdaseradiosolarnomparkuukojemseprezentirasedam
razliitih tehnologija, moe posluiti kao ogledni prostor za optimiranje uinkovitoski
tehnologije u odnosu na utjecaje na okoli i ekoloku mreu, za potrebe buduih solarnih
parkovanaslinimlokacijamauRepubliciHrvatskoj.
Kljunerijei:sunaneelektrane,sedamrazliitihtehnologija,utjecaji,okoli,ekolokamrea
SOLARPARKKOSOREENVIRONMENTALIMPACTANDNATURA2000NETWORK
N.Pavlus,M.Draki,D.valjek,M.Peri,D.egvi,M.Vukovi,B.Matoi,S.Blaevi
Zeleniservisd.o.o.,Split,Croatia(mihael.draksic@zeleniservis.hr)
SolarparkKosorewillbebuildwithinKosoreworkzone,inthetownofVrlika.Itisplannedto
build seven different photovoltaic power plants on the ground, each rated at 300 kW. The
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procedurewillallowproductionofelectricity,whichwillthenbedistributedintotheelectricity
network. Given that this is a solar park which represents seven different technologies and it
canbeusedasaprimeareafortechnologyeffectivenesoptimizationinrelationtotheimpacts
on the environment and Natura 2000 network, and also for the future solar parks in similar
locationsintheRepublicofCroatia.
P14
SIDRITEUUVALIMEZUPORAT,OTOKBIEVOKAKOSAUVATIEKOLOKUMREU?
N.Pavlus1,M.Peri1,M.Draki1,M.Vukovi1,S.Blaevi1,.Varga2,A.Ptiek2,H.izmek3
1
Zeleniservisd.o.o.,Split,Hrvatska(natalija.pavlus@zeleniservis.hr)
2
INSTITUTIGH,d.d.Zagreb,Hrvatska
3
JANOLUSd.o.o.,Zadar,Hrvatska
SidriteuuvaliMezuporat,naotokuBievujeplaniranoza20brodova,napovriniakvatorija
od 4500 m2, koji je dio ekoloke mree HR3000098 Bievo more. Sidrite se nalazi u blizini
ModrepiljejedinogodrijetkihzatienihobjektatevrstenaJadranukojiseposjeujeidioje
ekolokemree,HR3000184Modrapilja.Razlogzaizgradnjusidritajepritisakposjetiteljana
Modrupilju,kojirasteizgodineugodinu,uslijedegauljetnimmjesecimacijelauvalapostaje
prostor sve brojnijeg "divljeg" sidrenja brodova, ime se sustavno unitava morsko dno,
zatienebiljneiivotinjskevrste,kaodioekolokemreeRepublikeHrvatske.
Kljunerijei:Modrapilja,uvalaMezuporat,posjetitelji,utjecaji,ekolokamrea,sidrite
THEANCHORAGEINTHEMEZUPORATBAY,ISLANDOFBIEVOHOWTOPRESERVENATURA
2000NETWORK?
N.Pavlus1,M.Peri1,M.Draki1,M.Vukovi1,S.Blaevi1,.Varga2,A.Ptiek2,H.izmek3
1
Zeleniservisd.o.o.,Split,Croatia(natalija.pavlus@zeleniservis.hr)
2
INSTITUTIGH,d.d.Zagreb,Croatia
3
JANOLUSd.o.o.,Zadar,Croatia
The anchorage in the Mezuporat bay, on the island of Bievo is planned for 20 ships, in the
aquatorium measuring 4500 m2 of surface of which is the part of Natura 2000 network
HR3000098 Bievo more. The anchorage is located near the cave Modra one of the fewest
protected areas of this kind in the Adriatic, which is frequently visited and it is part of the
Natura 2000 network HR3000184 Modra pilja.The reason for building the anchorage is the
risingvisitorpressureontotheModracave,whichisgrowingeachyear,resultingintheimpact
of the "wild" ships anchoring through in the whole bay area in the summer months which
systematically destroys the seabed, protected plant and animal species all being a part of
CroatiaNatura2000network.
Keywords:Modracave,Mezuporatbay,visitors,impacts,Natura2000network,anchorage
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P15
KVALITETAVODEUVODOTOCIMAPOLJOPRIVREDNOG,MOVARNOGIUMSKOGPORJEJA
V.Peri,A.Koi,J.Horvati
Zavod za biokemiju i ekofiziologiju biljaka, Odjel za biologiju, Sveuilite Josipa Jurja
StrossmayerauOsijeku,Osijek,Hrvatska(vpersic@biologija.unios.hr)
Procjenaograniavajuihimbenikarastaalgiitrofikogstanjavodotokaipoplavnogpodruja,
kojakarakterizirajunepravilnihidrolokiuvjetiipromjenevodostaja,ukljuujerazliitepristupe
potrebne za prihvatljivo razumijevanje problema eutrofikacije. Cilj ovog rada je pruiti
informacije o prostornoj i vremenskoj raspodjeli hranjivih tvari i ograniavajuih imbenika
rasta algi, kako bi se stvorila znanstvena osnova za procjenu stanja trofije kao nunog
preduvjeta definiranja mjera za smanjenje optereenja voda hranjivim tvarima. Za postizanje
tog cilja dizajniran je eksperiment u svrhu kvantitativne procjene rasta algi nakon dodatka
hranjivih tvari, a kao rezultat dobivena je mjerljiva vrijednost ograniavajueg djelovanja
odreenehranjivetvari.Nainkoritenjazemljitakaoivrijemeuzorkovanjaimalisuznaajan
utjecajnautvrenuvarijabilnostfizikalnokemijskihimbenika,kaoipotencijalarastaalgi.U
skladustim,kvalitetavodauslivusnajviimpostotkompoljoprivrednogzemljitabilajevrlo
loasiznimnovisokimkoncentracijamafosfora.Samoblagiznakoviodstupanjaodreferentnih
vrijednosti zabiljeeni su u vodotocima preteno umskog sliva kao rezultat antropogenih
aktivnosti. S druge strane, u vodotocima movarnih slivnih podruja povremeno je utvren i
nedostatakhranjivihtvari.
Kljunerijei:eksperimentobogaivanjanutrijentima,trofikiuvjeti,N/Pomjer
V.Peri,A.Koi,J.Horvati
SubDepartmentofPlantEcophysiologyandBiochemistry/DepartmentofBiology,Universityof
Osijek,Osijek,Croatia(vpersic@biologija.unios.hr)
Assessmentofresourcesthatarelimitingalgalgrowthandevaluationofthetrophicstatusof
rivers and wetlands, which present hydrological irregularities, incorporates different
approaches in order to provide acceptable understanding of the problem. Our goal was to
provideinformationonthetemporalandspatialvariationofresourcelimitationofalgalgrowth
rates tocreateascientificbasisforevaluatingregionalstrategiesofnutrientmanagementin
theinvestigatedcatchments.Therefore,aresponsivetestwasdesignedtoassessquantitatively
algal growth after nutrient additions in order to obtain a biologically relevant measure of
nutrientdeficiency.Asaresult,abioassaymethodwasintroducedfortheevaluationoftrophic
statusandlimitingnutrientsintheinvestigatedwatercourses.Theobservedvariabilityinmany
ofthephysicalandchemicalconstituents,aswellasalgalgrowthpotential,wasattributedto
thesignificantinfluenceofbothcatchmentlanduseandsamplingtime.Accordingly,thewater
quality of watercourses in the catchment with the highest percentage of arable land was
determined to be very bad with extremely high phosphorus concentrations. Only moderate
signs of distortion were observed in flowing waters of forested catchments as a result of
anthropogenic activities. On the other hand, watercourses draining wetland watersheds
showedevensomenutrientdeficiency.
Keywords:nutrientenrichmentexperiment,trophicconditions,N/Pratio
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P16
EKOLOKA MREA NATURA 2000 TO SE PROMIJENILO NAKON BIOGEOGRAFSKOG
SEMINARA?
I.Plavac,A.Dupli,K.Jeli,J.Radovi,R.Topi,I.Ilija
Dravnizavodzazatituprirode,Zagreb,Hrvatska(ivana.plavac@dzzp.hr)
EkolokamreaNatura2000jemreaouvanihpodrujaEurope,namijenjenaouvanjuvieod
1000 ugroenih divljih vrsta i oko 230 staninih tipova. Dva su propisa temelj politike EU za
postizanjeouvanjabioraznolikostiDirektivaostanitimaiDirektivaopticama.Pristupanjem
EU, Hrvatska je predloila Natura 2000 podruja za vie od 250 vrsta i 70 staninih tipova
znaajnih za Uniju, koji se pojavljuju u Hrvatskoj i time znaajno doprinijela zaustavljanju
gubitka bioraznolikosti na razini Europe. Podruja mree odabrana su na temelju strunih
kriterija za pojedinu vrstu i stanini tip, prola su proces konzultacija s javnou te ih je
Uredbom u 2013. (NN 124/13) donijela Vlada RH. Kako bi zadovoljila zahtjeve Direktive o
stanitima, Hrvatska je morala proi postupak potvrivanja mree, koji se provodi u vidu
Biogeografskog seminara i za Hrvatsku je proveden u rujnu 2014. Na Seminaru su, osim
predstavnika DZZPa kao koordinatora inventarizacije divljih vrsta i staninih tipova i
predstavnikaEuropskekomisije,sudjelovaliipredstavniciNVOteneovisnistrunjaci.Temeljem
zakljuakaEKsaSeminara,DZZPusklaujepopisPodrujaouvanjaznaajnihzavrsteistanine
tipoveteihRHdostavljaEK.Kljunepromjeneupodrujimaekolokemreepredstavljenesuu
ovomradu,aobuhvaajudopunumreepodrujimarijekeKoraneiSaveuzvodnoodZagreba
tedopunupostojeihpodrujanovimciljnimvrstamaistaninimtipovima.
Kljunerijei:ekolokamrea,Natura2000,Biogeografskiseminar,Direktivaostanitima
NATURA2000NETWORKWHATHASCHANGEDAFTERTHEBIOGEOGRAPHICALSEMINAR?
I.Plavac,A.Duplic,K.Jelic,J.Radovic,R.Topic,I.Ilijas
StateInstituteforNatureProtection,ZagrebCroatia(ivana.plavac@dzzp.hr)
Natura2000networkisanetworkofconservationareasinEurope,aimedtoconservemorethan
1000threatenedspeciesandabout230habitattypes.ThecornerstonesofEUpolicyforachieving
biodiversity conservation are two Directives Habitats Directive and Birds Directive. By joining
EU, Croatia has proposed Natura 2000 areas for more than 250 species and 70 habitat types
present in Croatia, thus significantly contributing to halting the biodiversity loss in Europe. The
areas have been selected according to scientific benchmarks, undergone the process of public
consultationsandin2013promulgatedwiththeOrdinanceofCroatianGovernment.Tomeetthe
requirements of the Habitats Directive, Croatia went through the confirmation process,
conducted in the form of Biogeographical Seminar, which was carried out in September 2014.
ApartfromtheSINPrepresentativesasthespeciesandhabitatsinventorycoordinatorandthe
representatives of the European Commission, the representatives of NGOs as well as
independent experts participated in the Seminar. On the basis of the EC conclusions from the
Seminar,SINPisharmonisingtheSitesofCommunityImportancewhichtheRepublicofCroatiais
submittingtotheEC.TheessentialchangesinNatura2000areasarepresentedinthispaperand
theyincludetwonewsitesasanaddendumtothenetwork(RiverKoranaandUpperSava)aswell
asadditionofnewtargetspeciesandhabitattypestoexistingareas.
Keywords:Natura2000network,BiogeographicalSeminar,HabitatsDirective
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P17
ISTRAIVANJERIJETKIHIUGROENIHSVOJTIMALAKOFAUNEISTRE
I.Saboli,V.Dori,A.Dobrovi,A.Orlovi,P.Crnan,M.Rezo
Udruga studenata biologije, Prirodoslovnomatematiki fakultet, Zagreb, Hrvatska
(rezomartina2@gmail.com)
Kljunerijei:mekuci,ugroenevrste,Microcondylaeacompressa,Istrianamirnae,Istra
RESEARCHOFRAREANDENDANGEREDTAXAOFISTRIANMALACOFAUNE
I.Saboli,V.Dori,A.Dobrovi,A.Orlovi,P.Crnan,M.Rezo
Biology Students Association (BIUS), Faculty of Science, Zagreb, Croatia
(rezomartina2@gmail.com)
DuringtheprojectofmolluscsinventarisationinIstriacarriedoutbetween2012to2014,a
large number of rare and endangered species was recorded. Based on the level of threat
they are facing, five of them were chosen for this study: the freshwater Bivalvia Unio
mancus and Microcondylaea compressa, the freshwater Gastropoda Istriana mirnae,
terrestrial Gastropoda Vertigo angustior and Vertigo moulinsiana. The purpose of the
projectwastoexaminetheprevalenceandpopulationstatesofthechosenspeciesinIstria,
aswellasdeterminethefactorsthatpresentatreattothem,andsuggestpossiblemeasures
of protection. During the field work operations, usual methods for sampling of terrestrial
and freshwater molluscs were used. The samples were determined and distribution maps
weremadeforeachspecies.Theresultsshowthatthechosenspeciesarepresentinasmall
number of locations in Istria, and for some of them (i.e. Microcondylaea compressa, and
Istrianamirnae)thiswasthefirsttimetheirpresencewasconfirmedinCroatia.Thefactors
that present a threat to the investigated species are interventions on the watercourses,
polution, land reclamation, and alien molluscs species. For protection purposes, state of
populations of these species should be examined regulary, and all harmful factors
neutralizedorreduced.
Keywords:molluscs,endangeredspecies,Microcondylaeacompressa,Istrianamirnae,Istria
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P18
STRUKTURAIVIJABILNOSTPOPULACIJESRNE,CapreoluscapreolusUKONTINENTALNOJHRVATSKOJ
M.Saboli1,I.Buj2,M.Mrakovi2,M.aleta3
1
Dravnizavodzazatituprirode,Zagreb,Hrvatska
2
Zoologijskizavod,Biolokiodsjek,PMF,Zagreb,Hrvatska(ivaradic@biol.pmf.hr)
3
Uiteljskifakultet,Zagreb,Hrvatska
Kakobipredvidjelibuduepromjeneudinamicidivljihpopulacija,osobitovjerojatnostizumiranja,
potrebnesukvantitativneanalizetemeljenenapodacimaoekolokimpopulacijskimznaajkama.
Ovojeistraivanjeprovedenosciljemodreivanjaekolokihznaajkipopulacijesrnenalovituu
kontinentalnojHrvatskojikvantitativneprocjenepreivljavanjatepopulacije.Natemeljudobivenih
rezultata zakljuili smo da stanina struktura i antropoloke aktivnosti uvelike utjeu na
rasprostranjenost, populacijsku gustou i preivljavanje populacije srne. Istraivana je populacija
prometnicom podijeljena u dvije subpopulacije. Ukupan broj jedinki te gustoa i broj lanadi po
enki vei su u istonoj subpopulaciji, vjerojatno kao posljedica bolje kvalitete stanita (veeg
udjela uma) na podruju njenog obitavanja. Usporedbom utvrenih populacijskih parametara s
literaturnim podacima dolazi se do zakljuka da je kvaliteta stanita na cijelom istraivanom
podruju visoka, a populacijska gustoa zadovoljavajua, s obzirom da se radi o populaciji pod
lovnimpritiskom.Rezultatianalizevijabilnostiukazujunatankulinijuizmeustabilnepopulacijei
izumiranja,naglaavajuivanoststrogihkontrolaisprjeavanjakrivolova.
Kljunerijei:srna,strukturapopulacije,procjenavijabilnosti,lovnipritisak
STRUCTURE AND VIABILITY OF THE ROE DEER Capreolus capreolus POPULATION IN THE
CONTINENTALCROATIA
M.Saboli1,I.Buj2,M.Mrakovi2,M.aleta3
1
StateDepartmentforNatureProtection,Zagreb,Hrvatska
2
Department of Zoology, Faculty of Science, University of Zagreb, Zagreb, Croatia
(ivaradic@biol.pmf.hr)
3
FacultyofTeacherEducation,UniversityofZagreb,Zagreb,Croatia
Inordertopredictfuturealterationsofthedynamicsofwildanimalpopulations,inparticular
thepossibilityofextinction,quantitativeanalysesbasedontheinformationontheecological
populationparameters,arerequired.Thisinvestigationwasconductedinordertoestimatethe
populationparametersoftheRoedeerpopulationonthehuntinggroundinnorthernCroatia
and to quantitatively explore its viability. It was concluded that the habitat structure and
anthropologicalactivitieshavegreateffectonthedistribution,populationdensityandsurvival
oftheinvestigatedpopulation.Thatpopulationisdividedintotwosubpopulationsbyatraffic
road.Thetotalnumberofindividuals,populationdensityandthenumberoffawnsperfemale
are greater in the eastern subpopulation, probably due to the better habitat quality (greater
portion of forests) in its distribution range. Nevertheless, by comparing the population
parametersobtainedwithliteraturedata,itcanbeconcludedthattheenvironmentqualityis
high in the whole investigated area and that the population density is satisfactory for
populations which are under hunting pressure. Results of the viability analyses indicate that
the line between balanced population density and extinction is very thin for the Roe deer
populationsundertheanthropologicalinfluence,implyingtheimportanceofrigorouscontrol
andpreventionofillegalhunting.
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12.HBK15812 CBC
Keywords:Roedeer,populationstructure,viabilityestimation,huntingpressure
P19
OCJENA PROGRAMA RADOVA ODRAVANJA U PODRUJU ZATITE OD TETNOG
DJELOVANJA VODA U HRVATSKOJ UVJETI I MJERE ZATITE PRIRODE: TEMELJI, IZAZOVI I
PLANOVIZABUDUNOST
M.Saboli1,T.ukovi2,A.Dupli1,P.Lunko1,D.Schneider1
1
Odjel za ocjenu prihvatljivosti zahvata za prirodu, Dravni zavod za zatitu prirode, Zagreb,
Hrvatska(marija.sabolic@dzzp.hr)
2
Paljetkovaulica2,Zagreb,Hrvatska
Kljunerijei:uvjetiimjerezatiteprirode,Programradovaodravanjavoda,ocjenaprihvatljivosti
ASSESSMENTOFTHEPROGRAMMEFORMAINTENANCEWORKSFORPROTECTIONAGAINST
HARMFUL EFFECTS OF WATER IN CROATIA NATURE PROTECTION CONDITIONS AND
MEASURES:FUNDAMENTALS,CHALLENGESANDPLANSFORTHEFUTURE
M.Saboli1,T.ukovi2,A.Dupli1,P.Lunko1,D.Schneider1
1
Department for nature impact assessment, State Institute for Nature Protection, Zagreb,
Croatia(marija.sabolic@dzzp.hr)
2
Paljetkovaulica2,Zagreb,Croatia
12.HBK15912 CBC
specific measures were devised. Since 2012 problems in assessing the Programme are late
submission which can lead to the beginning of works before conditions are issued, deficient
works description and misinterpretation of measures/uninformed contractors. Plans for the
futurearefurthercooperationbetweenkeysectors,stakeholdereducation,realizationofonly
necessaryworks,bettersystemofmonitoringandreporting,monitoringefficiencyofmeasures
andtheirimprovementbasedontheresults,furtherelaborationofmeasuresandappropriate
assessmentatthestrategiclevel.
Key words: nature protection conditions and measures, Programme for water maintenance
works,appropriateassessment
P20
DRVENE OBALOUTVRDE: EKOLOKA ALTERNATIVA KAMENU I BETONU NA OBALAMA
VODOTOKA
R.Skukan,N.Koleti,L.Dereanin,N.Maleevi,R.pani,S.Dekani
Institutzaistraivanjeirazvojodrivihekosustava,VelikaGorica,Hrvatska(ires@ires.hr)
Kljune rijei: drvene obaloutvrde, upravljanje vodama, erozija obala, ekoloke metode
stabilizacijeobala
R.Skukan,N.Koleti,L.Dereanin,N.Maleevi,R.pani,S.Dekani
Institute for research and development of sustainable ecosystems, Velika Gorica, Croatia
(ires@ires.hr)
Sustainable water management in accordance with the Water Framework Directive includes
environmental,hydrotechnicalandcontrolmeasuresdesignedtomaintainorreintroducenatural
featuresofwatercourses.Oneofthekeyprocessesintheriverinenaturaldynamics,thatrequires
activemanagement,iserosionofriverbanks.Inthispaperweaimtodemonstratethepossibility
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12.HBK16012 CBC
Keywords:treerevetments,watercoursemanagement,coastalerosion,ecologicalmethodsof
riverbanksstabilization
2.SIMPOZIJEDUKACIJEBIOLOGIJE
2ndBIOLOGYEDUCATIONSYMPOSIUM
P21
PRIMJENAONLINETEAJAZAUENJEBIOLOGIJEUSREDNJOJKOLI
S.Fabac1,V.Fabijani2
1
GimnazijaVladimiraNazora,Zadar,Hrvatska(sanja.fabac1@optinet.hr)
2
SrednjakolaBartulaKaia,Pag,Hrvatska
Onlinealatisesvevieprimjenjujuunastavnomprocesu.Eobrazovanjepostajetehnoloki
imperativ, a uvode se nove metode uenja koje e uenike pratiti kroz daljnje kolovanje.
Onlineteajeviukljuujumoderneelementeuenjaipouavanjauodnosunatradicionalnu
nastavu. Uenik ui po vlastitom tempu, u vrijeme koje mu odgovara. Pretpostavka je da
primjena online teaja doprinosi boljem razumijevanju i poveanom interesu za nastavne
sadraje iz biologije. Pomou online teajeva uspostavlja se bolja komunikacija meu
uenicima preko razmjene iskustava i ideja na forumima. Takoer se uspostavlja i bolja
komunikacija uenika s nastavnicima kroz razmjenu pitanja i odgovora. Dodatno motivira i
mogunost vrednovanja znanja. U teaju je omogueno da nastavnici evaluiraju ishode
uenjapolaznika.Ciljistraivanjabiojeusporeditirezultateuenika1.i3.razredaGimnazije
VladimiraNazoraizZadraiSrednjekoleBartulaKaiaizPaga,kojiimajupodjednakuspjeh
iz biologije. Uenici kontrolne skupine nisu koristili online alate, a uenici pokusne skupine
bilisuaktivnisudionicionlineteaja.Provedenajeianketaspitanjimazatvorenogtipameu
uenicimapokusneskupine.Obradapodatakapotvrdilajenauhipotezu:(1)dajepovean
intereszasadrajeizbiologijei(2)dasupostignutiboljirezultati.Stogamoemozakljuitida
sekoritenjemonlineteajakoduenjalakepostiuciljeviiishodinastavebiologije,tese
unaprjeujukognitivnapostignuauenika.
Kljunerijei:Onlineteaj,ciljeviiishodi,kognitivnapostignua,biologija
APPLICATIONOFONLINESEMINARFORSTUDYINGOFBIOLOGYINHIGHSCHOOL
S.Fabac1,V.Fabijani2
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12.HBK16112 CBC
HighSchoolVladimirNazor,Zadar,Croatia(sanja.fabac1@optinet.hr)
HighSchoolBartulKai,Pag,Croatia
Theonlinetoolsarebeingmorefrequentlyappliedineducationprocess.Ourpresumptionis
thattheapplicationoftheonlineseminarscontributestoabetterunderstandingandtoan
increasedinterestforeducationalcontentsofbiology.Viaonlineseminars,itisestablished
abettercommunicationbetweenpupilsthroughexchangeofexperiencesandideasonthe
forums. Also, a better communication is established between pupils and teachers through
exchangeofquestionsandanswers.Theaimofthisinvestigationwastocomparetheresults
oflearningbetweenpupilsofthe1st and3rdclassoftheHighSchoolVladimirNazor,Zadar
and those from the High School Bartul Kai, Pag, which have attained almost the same
success in learning biology. The pupils from the control group did not use the online tools
whilethepupilsfromtheexperimentalgroupactivelyparticipatedintheonlineseminar.We
havealsoperformedthequestionnaireincludingofthequestionsofthe"closedtype"(blind
type) among the pupils from the experimental group. Data analysis confirmed our
hypothesisthattheonlinelearningresultedin:(1)anincreasedinterestforbiologycontents
and(2)betterresultsoflearning.Accordingtothat,weconcludedthattheuseoftheonline
seminarforlearningmayfacilitatetheattainingofobjectivesandoutcomesintheeducation
ofbiologyandmayimprovethecognitiveaccomplishmentsofpupils.
Keywords:onlineseminar,objectivesandoutcomes,cognitiveaccomplishments,biology
P22
USVOJENOST OSNOVNIH KONCEPATA IZ BOTANIKE KOD UENIKA PETOG RAZREDA
OSNOVNEKOLE
N.Kleteki1,D.Novoseli2
1
OBogumilaTonija,Samobor,Hrvatska(natasa.kletecki@gmail.com)
2
Alfad.d.,Zagreb,Hrvatska
Cilj istraivanja bio je utvrditi usvojenost osnovnih koncepata botanike koje su uenici
usvojilinakrajuprvogobrazovnogciklusaiznastavnogpredmetaPrirodaidrutvouniim
razredimaosnovnekole.Istraivanjejeprovedenosuenicima5.razredaosnovnekole,a
prije obrade nastavne teme "Biljka cvjetnjaa" nastavnog predmeta "Priroda". Instrument
istraivanja sastojao se od niza zadataka objektivnog tipa. Sadravao je ukupno 26 pitanja
koja su ispitivala tri kognitivne razine znanja: reproduktivno znanje (prva razina),
konceptualno razumijevanje (druga razina) te rjeavanje problema (trea razina). Zadaci su
bili otvorenog tipa (nadopunjavanja, dosjeanja i esejskog tipa) te zatvorenog tipa
(alternativnog izbora, viestrukog izbora, povezivanja ujednaenog tipa). Utvrena je
nedovoljnausvojenostosnovnihkoncepatabotanike.Ueniciboljerjeavajuzadatkekojima
seispitujereproduktivnoznanje,amanjesuuspjeniurjeavanjuzadatakakojimaseispituju
viekognitivnerazineznanja.
Kljunerijei:priroda,botanika,niirazrediosnovnekole,koncepti,kognitivnerazine
N.Kleteki1,D.Novoseli2
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12.HBK16212 CBC
BogumilToniElementarySchool,Samobor,Croatia(natasa.kletecki@gmail.com)
Alfad.d.,Zagreb,Croatia
Theaimofthisresearchwastodeterminetheacquisitionlevelofbasicbotanyconceptsthat
studentsadoptedbytheendofthefirsteducationalcycleinthesubject"NatureandSociety"
during the first four years of their formal education. Research was conducted among
elementary school students in the fifth grade, in Nature Class, before the chapter "The
Flowering Plant". The survey instrument consisted of 26 objective test items that assessed
three levels of cognitive domain: recalling knowledge (first level), conceptual understanding
(second level) and problem solving (third level). Tasks were openended (short answers,
recalling facts and essays) and fixedchoice (alternative choice, multiple choice, matching).
Resultsshowanunsatisfactorypermanenceofknowledgeofbasicbotanyconcepts.Students
were more successful at solving tasks that tested knowledge, and less successful at solving
tasksthatrequiredhigherthinkingskills.
Keywords:nature,botany,primaryschool,elementaryschool,concepts,cognitivedomain
P23
USVOJENOST NASTAVNOG SADRAJA PRIMJENOM KONCEPTUALNIH MAPA KOD UENIKA
SREDNJEKOLE
K.Latin1,E.Merdi2,M.Nit3,I.Labak2
1
OsnovnakolaVladimirNazor,epin,Hrvatska(kristin.latin@gmail.com)
2
Odjelzabiologiju,Osijek,Hrvatska
3
Agencijazaodgojiobrazovanje,Osijek,Hrvatska
Cilj istraivanja bio je usporediti nivo usvojenog znanja steenog primjenom konceptualnih
mapasnivoomusvojenogznanjasteenogtradicionalnim(frontalnim)oblikomrada.Takoer
se ispitao i nivo retencije znanja. U istraivanje su bili ukljueni uenici drugih razreda
PrirodoslovnomatematikegimnazijeuOsijeku(101uenik).Tijekomtravnjaisvibnjakolske
godine 2013./2014. provedeni su nastavni sati s obradom nastavnih tema "Ptice" i "Sisavci".
Nastavnetemeudvasurazredaobraenefrontalnimoblikomrada,audvarazredapomou
konceptualnih mapa nakon ega je uslijedilo testiranje uenika pisanom provjerom znanja. U
rujnu sljedee kolske godine (2014./2015.) proveden je test retencije znanja sa svim
uenicima. Istraivanjem je utvreno da uenici koji su u uenju koristili konceptualnu mapu
postiustatistikiznaajnoboljerezultateuodnosunauenikekojisusadrajuilifrontalnim
oblikom rada. Isti rezultat je dobiven i nakon provedenog testa retencije. Ovim radom
prikazana je mogunost primjene konceptualnih mapa u uenju i pouavanju sadraja iz
Biologije kao efikasne nastavne metode koja pridonosi postizanju konceptualnog
razumijevanja.
Kljunerijei:konceptualnamapa,retencijaznanja,biologija
K.Latin1,E.Merdi2,M.Nit3,I.Labak2
1
PrimarySchoolVladimirNazor,epin,Croatia(kristin.latin@gmail.com)
2
TheDepartmentofBiology,theUniversityofOsijek,Osijek,Croatia
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12.HBK16312 CBC
EducationandTeacherTrainingAgency,Osijek,Croatia
The aim of this research was to explore whether the students achieve better results during
their learning process using conceptual maps or expository teaching. Also, the level of
knowledgeretentionwastested.Thisresearchwasconductedonthesampleof101studentsof
secondgradeofNaturalSciencesHighSchoolandMathematicsHighSchoolinOsijek.During
April and May in the school year 20132014 students have been teaching about two topics:
"Bird" and "Mammals". In two classes students used traditional way of learning, socalled
directinstruction.Inothertwoclassesstudenttaught bythemselvesusingconceptual maps.
After teaching period was completed, all students wrote final test. Next school year (2014
2015) in September the knowledge retention test was carried out with all students. This
research showed that students who used conceptual maps during learning process achieve
statistical significant better results compared to students who have teach by using direct
instructions.Thesameresultisobtainedaftertheretentiontest.Thispaperpresentsthegood
possibility for applying the conceptual maps in learning and teaching biology topics as an
effectiveteachingmethodthatcontributestotheachievementofconceptualunderstanding.
Keywords:conceptualmap,retentionofknowledge,biology
P24
UTJECAJ KOGNITIVNIH RAZINA ZADATAKA NA VJERODOSTOJNOST
USVOJENOSTIKONCEPATAGENETIKEKODGIMNAZIJSKIHUENIKA
PROCJENE
M.Mihali,H.Kostadinovi
Prirodoslovnomatematikifakultet,Zagreb,Hrvatska(marija.mihalic114@gmail.com)
Cilj ovog rada bio je istraiti kako na vjerodostojnost provjere usvojenosti nastavnih koncepata
utjee struktura zadataka postavljenih u tu svrhu. Analiziran je 141 test, to predstavlja 10%
najbolje rijeenih testova sa upanijskog natjecanja iz biologije za IV. razred gimnazije,
provedenog2014.godine,napodrujucijeleHrvatske.Izdvojenasutrizadatkaijajerijeenost
bila najloija, a koji su obuhvaeni nastavnom cjelinom genetika. Utvreno je koje koncepte
ispitujunavedenizadaci,atosu:1)Kodiranjezaaminokiseline;2)Kretanjealelaupopulaciji;3)
Nastanakrekombinantnihpotomaka.Zatimsusastavljeninovitestovikojejeiniloukupnodevet
zadataka. Ti zadaci su postavljeni tako da se pomou njih provjerava usvojenost utvrenih
koncepata na sve tri razine znanja (svaki od tri koncepta ispitan je s tri nova pitanja, svako na
jednojodtrirazineznanja).NovitestovisudaninarijeavanjeuenicimaetvrtihrazredaV.iXV.
gimnazije u Zagrebu (ukupno 54 uenika). Iz dobivenih rezultata moe se izvui zakljuak da
vjerodostojnost procjene usvojenosti koncepata dobrim dijelom ovisi o pristupu ispitivaa u
oblikovanjuzadataka.Istotakomoemozakljuitidarijeenostzadatakaviihkognitivnihrazina
nemorabitigarancijazapotpunuusvojenostkonceptaiostvarenjeplaniranihishodauenjana
niim razinama. Da se ostvari to sigurnija procjena usvojenosti odreenog koncepta od strane
uenika,poeljnojeispitatitajkonceptnasvetrirazineznanja.
Kljunerijei:kognitivnarazina,koncept,zadatak
IMPACTOFCOGNITIVELEVELSOFTASKSONGENETICSCONCEPTSASSESSMENTCREDIBILITY
INHIGHSCHOOLSTUDENTS
M.Mihali,H.Kostadinovi
FacultyofScience,UniversityofZagreb,Zagreb,Croatia(marija.mihalic114@gmail.com)
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12.HBK16412 CBC
Theaimofthisstudywastoinvestigatehowthestructureoftasksaffectsconceptsassessment
credibility.141testswereanalyzed,whichrepresents10%ofthebestsolvedtestsinCroatian
NationalBiologyCompetitionfor12thgrade(4.highschoolgrade)students,conductedin2014.
Three worst solved tasks from genetics topic were observed. Concepts that evaluate those
tasks were determined, and they are: 1) Amino acid coding; 2) Movement of alleles in a
population; 3) Recombinant offspring production. New tests with nine tasks were prepared.
Thesetaskswereusedtoevaluateadoptionoftheestablishedconceptsatallthreecognitive
levelsofknowledge(eachofthethreeconceptswasevaluatedwiththreenewtasks,eachtask
on one of the three cognitive levels). New tests were given to 12th year students of V.
GimnazijaandXV.GimnazijainZagreb(54studentstotal).Itcanbeconcludedfromtheresults
thatconceptassessmentcredibilitydependsonstructureoftasks.Wecanalsoconcludethat
solvingoftasksonhighercognitivelevelsmaynotbeaguaranteeforrealizationofintended
learningoutcomesatlowercognitivelevels.Itisdesirabletoexaminetheconceptatallthree
cognitivelevelsofknowledge,tomakesurestudentshaveadoptedtheconceptsproperly.
Keywords:cognitivelevel,concept,task
P25
KORITENJELOKALNIHRESURSAUTERENSKOJNASTAVIBIOLOGIJE
M.Raan1,S.Mikuli2,D.Sumpor3,M.LabaHorvat1
1
SrednjakolaPrelog,Prelog,Hrvatska(rasanmiso@gmail.com)
2
GimnazijaFranGalovi,Koprivnica,Hrvatska
3
SrednjakolaTinaUjevia,Kutina,Hrvatska
USrednjojkoliPrelog,GimnazijiFranGaloviiSrednjojkoliTinaUjeviajeuokviruizborne
nastave biologije u drugim i treim razredima gimnazije zastupljen terenski rad. Nastava
ekologije, botanike i zoologije na terenu je vezana uz istraivake uenike projekte. Za
uenika istraivanja ivog svijeta koriteni su prirodni ekosustavi. Cilj rada je ispitati stavove
uenikaokompetencijamakojesteknutijekomvlastitogistraivanjakoristeiekosustavesvog
okruenja. Kontrolnu skupinu ine uenici drugih i treih razreda gimnazije koji nisu radili
terenskoistraivanje,aizbiologijesuocjenjeniocjenomvrlodobariliodlian.Eksperimentalnu
skupinuineuenicidrugihitreihrazredanavedenihkolakojisuradiliterenskoistraivanje.
Pretpostavka je da e uenici koji su radili terenska istraivanja cjelovitije, svrsishodnije
sagledavatiiobjasnjavatiuzronoposljedinevezeipovezivati skognitivnimpostignuima te
znatipovezatisaprimjenomusvakodnevnomivotu.Zaistraivanjejekoritenaanketaukojoj
suispitaniciodgovaralinapitanjazatvorenogtipabrojanopremaskalipoLikertu(1uopene;
2uglavnomne;3nisamsiguran;4uglavnomda;5upotpunosti).Obradomrezultataankete
ustanovljeno je da je eksperimentalna skupina izrazila pozitivnije statove prema uspjesima
baziranih na koritenju lokalnih prirodnih resursa u uenikim istraivakim projektima.
Nastava prirodnih znanosti trebala bi se vie usredotoiti na metodu istraivakog rada
koristei lokalne prirodne resurse. Kvalitenije usvojeni koncept odrivog razvoja je temelj za
aktivnije ukljuivanje u rjeavanje problema u lokalnoj zajednici. Uenici eksperimentalne
grupe smatraju da je rad na istraivakom projektu poboljao razvoj njihovih govornikih
vjetina. Pri provjeri znanja o ekosustavima koji ne pripadaju lokalnom prirodnom okoliu
uenicieksperimentalneskupinesupokazaliboljerezultate.
Kljunerijei:uenikiistraivakiprojekt,terenskirad,kompetencije
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12.HBK16512 CBC
USINGLOCALRESOURCESINBIOLOGYFIELDWORK
M.Raan1,S.Mikuli2,D.Sumpor3,M.LabaHorvat1
1
HighSchoolPrelog,Prelog,Croatia(rasanmiso@gmail.com)
2
HighSchoolFranGalovi,Koprivnica,Croatia
3
HighSchoolTinaUjevia,Kutina,Croatia
InSecondaryschoolPrelog,GrammarschoolFranGaloviandSecondaryschoolTinUjevifield
workisapartofelectiveBiologyclassesforstudentsinthesecondandthirdgrade.Teaching
ecology, botany and zoology in the field is connected to student research projects. For the
student research of the living world, natural ecosystems have been used. The topic of the
paper is to examine student attitudes towards competencies that they acquire during their
individularesearchwhileusingecosystemsintheirsurroundings.Thecontrolgroupismadeup
ofthesecondandthirdyeargrammarschoolstudentswhohaveneverdonefieldresearchand
have an A or B in Biology. The experimental group is made up of the second and third year
studentsfromabovementionedschoolswhohavedonefieldresearch.Theassumpionisthat
thestudentswhohavedonefieldworkwilllookatproblemsmorethoroughlyandwithmore
sense and will be able to explain cause and effect relationships, connect it to cognitive
achievements and will know how to connect it to everyday situations. For the sake of
research,apollwasusedwheretheexamineesansweredclosedtypequestionsontheLikert
scale (1 not at all; 2 mostly no; 3 not sure; 4 mostly yes; 5 completely). After data
processing,theresultsshowedthattheexperimentalgrouphasstatedmorepositiveattitudes
towards accomplishments based on usage of local natural resouces in student research
projects. Science classes should focus more on the method of research study, using local
naturalresourcesleadstoaqualityunderstandingofsustainabledevelopmentwhichleadsto
more active involvement in the political life of a grownup responsible citizen. Experimental
group students think that working on a research project has lead to development of
independence and security in presenting. Experimental group of students has shown better
results during tests when examined about ecosystems which do not belong to local
environment.
Keywords:students'researchprojects,fieldresearch,competencies
P26
IGRAUPONAVLJANJUNASTAVNIHSADRAJAVEZANIHUZIVOTNEZAJEDNICEMORA
J.RazlogGrlica1,N.Kleteki2,M.Novosel3
1
OIvaneBrliMaurani,Virovitica,Hrvatska(jasna.razloggrlica@vt.tcom.hr)
2
OBogumilaTonija,Samobor,Hrvatska
3
Prirodoslovnomatematikifakultet,Biolokiodsjek,Zagreb,Hrvatska
U nastavi prirode 6. razreda obrauju se teme vezane uz more u nastavnoj cjelini "ivotne
zajednicemoraivodanakopnu".Kodponavljanjasadrajaizovogpodrujauiteljimajeteko
postiimaksimalnuuenikukoncentracijuiaktivnostsvihuenika,pogotovoakosesatsvede
naklasinopropitivanje.Upravozatoseumjestoklasinihmetodaponavljanjauvodiigra.Ciljje
unaprjeivanje kvalitete nastavnog procesa i uspostavljanje aktivnijeg sudjelovanja uenika u
usvajanju nastavnih sadraja vezanih uz more kao stanite u 6. razredu. Tijekom provoenja
ponavljanja gradiva primjenjuju se igre asocijacije, krialjke i rebusi, koje uenici rjeavaju u
grupama.Usporeujuseirezultatianketiranjapo30uenika6.razredaizOBogumilaTonija
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12.HBK16612 CBC
SamoboriOIvaneBrliMauraniVirovitica,imeseispitujemotiviranostuenikainjihovo
zadovoljstvo ovakvim nainom rada. Uenici O Bogumila Tonija Samobor su tijekom obrade
nastavnih sadraja vezanih uz more koristili zbirku morskih organizama i edukacijsku knjiicu
"More je ivo" Drutva za ekoloka istraivanja Paks s popratnim video materijalima dok su
ueniciOIvaneBrliMauraniViroviticakoristilisamozbirkumorskihorganizama.Vrijednost
primjene igre u nastavi prirode je u tome to se kod uenika, razvija osjeaj zadovoljstva
usvojenim znanjem uz pozitivan natjecateljski duh kojim se potie usmena komunikacija,
tolerancija,uisluatiijaasamopuzdanjeuenika.Naovajnainuenicibreilakeusvajaju
sadrajeiuesetimskomradu.
Kljunerijei:ponavljanje,igra,osnovnakola,ivotnezajednicemora
THEPLAYINREPEATINGTHEEDUCATIONALCONTENTSABOUTMARINELIFE
J.RazlogGrlica1,N.Kleteki2,M.Novosel3
1
'IvanaBrliMaurani'Primaryschool,Virovitica,Croatia(jasna.razloggrlica@vt.tcom.hr)
2
'BogumilToni'Primaryschool,Samobor,Croatia
3
FacultyofScience,BiologicalDepartment,Zagreb,Croatia
Inprimaryschoolstopicsaboutmarinelifeareelaboratedinthe6thgradenatureeducation,in
the educational unit "Marine and freshwater life communities". It is difficult for teachers to
achieve maximum pupils' concentration and activity while repeating the contents of this topic,
especiallyiftheclassisdoneinawayofclassicalknowledgetesting.Itisforthisreasonthatthe
playisintroducedintothelearningprocess.Thegoalistoimprovetheeducationalprocessandto
establish a more active participation of pupils in acquiring the educational contents related to
marinehabitatsinthe6thgrade.Inrepeatingofthesecontents,gamesofassociation,crosswords,
diceandrebusareintroducedintothelearningprocess.Resultsofapolltakenamong30pupils
ofthe6thgradefromthe'BogumilToni'PrimaryschoolinSamoborand30pupilsofthe6thgrade
from the 'Ivana BrliMaurani' Primary school in Virovitica are compared, examining their
motivationandtheirsatisfactionwiththiswayoflearning.Pupilsfromthe'BogumilToni'Primary
schoolinSamoborintheirclassesaboutmarinelifealsousedacollectionofseaorganismsandan
educationalbooklet"Theseaisalive"publishedbytheSocietyforecologicalresearchPakswith
accompanying video material, while pupils from the 'Ivana BrliMaurani' Primary school in
Viroviticausedonlyacollectionofseaorganisms.Thevalueofplayinteachingnaturecontents
lies in the fact that pupils develop a capability of oral communication and tolerance, learn to
listen, strengthen their selfconfidence, develop a sense of satisfaction with the acquired
knowledgeandencouragetheircompetitiveskills.Inthiswaypupilslearnfasterandmoreeasily
acquireeducationalcontents,aswellaslearnhowtoworkinteam.
Keywords:repeating,play,primaryschool,marinelife
P27
EKOLOKETEMEUUDBENICIMAREPUBLIKEHRVATSKEISAVEZNEREPUBLIKENJEMAKE
.RomtajnBurchards,I.Bogut,.Popovi
Fakultetzaodgojneiobrazovneznanosti,SveuiliteJosipaJurjaStrossmayerauOsijeku,Osijek
(ibogut@foozos.hr)
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12.HBK16712 CBC
Kljunerijei:Nacionalniobrazovnikurikul,HrvatskaiNjemaka,udbenici,okoli,prirodaidrutvo
.RomtajnBurchards,I.Bogut,.Popovi
FacultyofEducation,JosipJurajStrossmayerUniversityofOsijek,Osijek(ibogut@foozos.hr)
European educational policy focused on developing competencies of students emphasizes the
importance of integration of environmental competencies in national framework curricula.
Competences of students in the field of environmental issues include understanding world of
nature, the principles of sustainable development, a critical understanding of science and
technology in terms of ethics, safety and environmental protection etc. The paper analyzes
contentsofthenationaleducationalcurricula(NOKandNRW),e.g.:competencesoflowergrade
primaryschoolstudentsonenvironmentalissues,andanalyzestextbooksforNatureandSociety
in Croatia and Germany (NordrheinWestfalen province) in order to compare presence of
environmental topics in them. It can be concluded that in both curricula pupils competencies
reflectingenvironmentalliteracyareverywellrepresented.Resultsofquantitativeanalysisshow
that the Croatian Nature and Society textbooks 1st4th grade is up to six environmental topics,
contrarytotwentyinGermantextbooks.Someobserveddifferencesintheapproachtoparticular
topicswerediscussedaswell.Weassumethataholisticapproachtoenvironmentalissuesinthe
National Curriculum of Germany and their textbooks, are partly a consequence of the
environmental policy of the EU which promotes standards based on action programs. It is
importanttoprovideconditionsinwhichpupilscandeveloptheirdailyenvironmentalabilities.
P28
UENIKOPOZNAVANJEIVOTINJAHRVATSKE
M.Srko1,I.tefani1,R.HalapirFrankovi2
th
12.HBK16812 CBC
Cilj istraivanja bio je analizirati koliko uenici poznaju injenice o ivotinjama koje ive u
Hrvatskojtejepostavljenahipotezadapoznavanjeivotinjaovisionastavnomprogramu,pri
emuenajboljerezultateostvaritiuenici3.razreda.Provedenoje2013.godinenauzorku
od127uenikaod1.do4.razredaV.gimnazijeuZagrebu.Uenicisurjeavalianketus2tipa
pitanja. U prva 3 pitanja traeno je ope znanje o ivotinjama Hrvatske, a u sljedeih 16
uenicisutrebalizaokruitijesulinavedenetvrdnjeoivotinjamatoneilinetone.Pitanja
su podijeljena u kategorije:a)jesuliilinisu u nastavnom planuiprogramu,b)na onakoja
jesu ili nisu vana za ivot uenika, c) pitanja vezana uz raznolikost te d) pitanja vezana uz
prilagodbeivotinja.Natemeljudobivenihpodatakamoeseuoitidasunajslabijerezultate
postigli uenici 1. razreda, a najbolje uenici 3. razreda. Nisu uoene razlike s obzirom na
spol te se moe zakljuiti da djevojice i djeaci podjednako poznaju ivotinje Hrvatske.
Ukupnarijeenostodoko60%upuujenazakljuakdabiseunastavibiologijetrebalovie
panje posvetiti toj tematici kako bi uenici shvatili vanost upoznavanja odreenih vrsta i
njihovih karakteristika to zbog ivotne vanosti (prepoznavanje zmija otrovnica) to zbog
opekulture(autohtonepasmineHrvatske).
Kljunerijei:istraivanje,V.gimnazija,ivotinjeHrvatske,analizaanketa
STUDENT`SKNOWLEDGEOFANIMALSTHATLIVEINCROATIA
M.Srko1,I.tefani1,R.HalapirFrankovi2
1
FacultyofScience,UniversityofZagreb,Zagreb,Croatia(mateja.srkoc@gmail.com)
2
FifthGymnasium,Zagreb,Croatia
Theaimofthisstudywastoanalyzehowmuchfactsthestudentsknowabouttheanimals
thatliveinCroatiaandithasbeenhypothesizedthatknowledgeofanimalsdependsonthe
curriculum.Thebestresultsachieved3rdgradestudents.Thestudywasmadein2013ona
sample of 127 students into 4 grades in V. High School in Zagreb. Students were solving 2
types of survey questions. In the first three questions they were asked about general
knowledge of animals in Croatia, and in the following 16, students had to choose whether
the following statements about animals are correct or incorrect. The questions are divided
intocategories:a)tothosethatareorarenotinthecurriculum,b)tothosethatareorare
notrelevanttothelifeofstudents,c)questionsrelatedtodiversityandd)questionsrelated
totheadaptationofanimals.Basedontheresults,thelowestscorehad1stgradestudents,
and the best score had students of 3rd grade. There is no essential difference between the
knowledge of girls and boys. The total score of about 60% indicates that biology teachers
should pay more attention to this topic so that students understand the importance of
gettingtoknowcertainspeciesandtheircharacteristics.Becausethatisveryimportantfor
everystudent(forexample,studentsshouldbeabletoidentifyingpoisonoussnakes).
Keywords:study,V.HighSchool,animalsofCroatia,analisisofsurvey
P29
STAVOVIUITELJAOBIOLOKIMNASTAVNIMSADRAJIMAUOSNOVNOJKOLI
A.ari,M.Mari
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12.HBK16912 CBC
CiljistraivanjabiojeutvrditistavoveuiteljaPrirodeiBiologijeoNastavnomplanuiprogramu
uosnovnojkoli.180uiteljaurazdobljuod2004.do2006.godineizraavalisuslaganjeuz18
pitanja anketnog upitnika vezanih za nastavni program Prirode i Biologije. Nastavni plan i
program itekako utjee na uenika postignua te na njihove interese i stavove prema
predmetu(citat).84%uiteljaseizjasnilodasezadaeuNastavnomplanuiprogramutemelje
na injenicama.46% uitelja smatra da se novi koncepti ne izgrauju prema usvojenim
predkoncepcijama. Uitelji su se takoer izjasnili da uenici ne razumiju gradivo o
razmnoavanju u 5 razredu te njih 67% smatra da uenici to gradivo ne mogu povezati sa
stvarnimivotomirazvojemstavapremavlastitomzdravlju.Veinauitelja,njih73%smatrada
bitrebalopromijenitipostojeiNastavniplaniprogram.SadanjiNastavniplaniprogramnijeu
dovoljnojmjeriprilagoendobiiinteresimauenikatenepodupireizgradnjunovihkoncepata
nausvojenimpredkoncepcijama.SadrajiNastavnogprogramabitrebalipodupiratiusvajanje
prirodoznanstvenemetodeikritikipoglednasvijetnaprincipuaktivnoguenja,toveibroj
profesorauanketismatradanijezastupljenousadanjemNastavnomprogramu.
Kljunerijei:kurikulum,uitelji,interesi,uenici
A.ari,M.Mari
FacultyofScience,UniversityofZagreb,Zagreb,Croatia(maricmonika@ymail.com)
Theaimofthisstudywasdetermingattitudesofelementaryschoolteacherstowardthebiology
curriculum.From2004to2006,180elementaryschoolbiologyteacherstookpartinthisstudy.
They answered to a yes/no questionnaire, which contains 18 questions related to the biology
curriculum.Thecurriculumhasamajorinfluenceonstudents'achievementsandtheirinterests
andattitudestowardtheschoolsubject(quote).84%ofteachersstatedthatthehomeworktasks
in the curriculum are based on solely theoretical knowledge. 46% of teachers reported that
studentsoftendonotusetheoreticalknowledgeforacquisitionofnewknowledge.Inaddition,
studentsin5thgradedonotcomprehendthelessionsabouthumanreproduction,thereforethey
can not apply this knowledge in real life and do not see the benefits of it. 73% of teachers
considered that the existing curriculum should be changed. The current curriculum is not
sufficiently arranged to the age and interests of the students and does not encourage the
developement of new knowledge. The content of the curriculum should support studying a
naturalscientific method, have a critical view and encourage active learning. Many teachers in
thissurveyagreewithmethodsnotbeingrepresentedinthecurrentcurriculum.
Keywords:curriculum,students,biology,teachers
EVOLUCIJA,SISTEMATIKA,FILOGENIJAIBIOGEOGRAFIJA
EVOLUTION,SYSTEMATICS,PHYLOGENYANDBIOGEOGRAPHY
P30
THE POTENTIAL ROLE OF ECOLOGICAL ADAPTATION AND Wolbachia INFECTION IN THE
DIVERSIFICATIONOFRhagoletiscerasi
th
12.HBK17012 CBC
V.Bakovi1,M.Schebeck1,C.Stauffer1,G.Ragland2,H.Schuler3
1
Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences,
Vienna,Austria(vid.bakovic@boku.ac.at)
2
DepartmentofEntomology,KansasStateUniversity,USA
3
DepartmentofBiologicalSciences,UniversityofNotreDame,USA
Keywords:sympatricspeciation,hostinduceddifferentiation,cytoplasmicincompatibility
P31
PRIMJENASUVREMENIHTEHNIKAPROSTORNESTATISTIKEUEKOLOKIMISTRAIVANJIMA
N.BiliDujmui,B.K.Hackenberger
Zavod za kvantitativnu ekologiju/Odjel za biologiju, Sveuilite Josipa Jurja Strossmayera u
Osijeku,Osijek,Hrvatska(nina.bilic.du@gmail.com)
Bitandiokvantitativnogpristupaekolokimistraivanjimajeprostornakomponenta.Stogasumetode
prostornog odreenja, bez obzira radi li se o veliini areala vrste ili koncentraciji nutrijenata ili
polutanata u nekom okolinom kompartmentu, nezaobilazan korak u interpretaciji istraivanja.
Razvojem informatike znanosti paralelno s tehnologijom otvorile su se mogunosti vrlo sloenog
simuliranja ekolokih eksperimenata to je dovelo do razvoja novih tehnika njihovog dizajniranja.
Zahvaljujui suvremenim metodama prostorne statistike mogue je postii optimizaciju ekolokih
istraivanjausmislureduciranjapotrebnogvremena,potrebneveliineuzorakaiveliineobuhvaenog
prostora. U ovom radu e biti prikazana primjena simulacije temeljene na prostorno statistikim
modelima i simulacijama uzorkovanja faune tla. Usporedbom rezultata dobivenih simuliranim
eksperimentom u laboratoriju i realnim eksperimentom u okoliu ustanovila se statistiki znaajna
slinost.Uokviruradadonesenesuhipotezeoporijeklugrekesimuliranogirealnogrezultata.
Kljunerijei:prostornastatistika,ekologija,faunatla
APPLICATIONOFMODERNSPATIALSTATISTICALTECHNIQUESINECOLOGICALRESEARCH
N.BiliDujmui,B.K.Hackenberger
Subdepartment of Quantitative Ecology/Department of Biology, University of Josip Juraj
StrossmayerinOsijek,Osijek,Croatia(nina.bilic.du@gmail.com)
th
12.HBK17112 CBC
Keywords:spatialstatistics,ecology,soilfauna
P32
GENETSKA STRUKTURA BJELONOGOG RAKA (Austropotamobius pallipes KOMPLEKS)
OTKRIVAMOGUUPOVEZANOSTIZMEUTRIKRKERIJEKETIJEKOMGEOLOKEPROLOSTI
M.Jeli1,F.Grandjean2,M.Govedi3,G.Klobuar1,D.Guyonnet4,I.Maguire1
1
Sveuilite u Zagrebu, Prirodoslovnomatematiki fakultet, Bioloki odsjek, Zoologijski zavod,
Zagreb,Hrvatska(misel.jelic@biol.pmf.hr)
2
Universit de Poitiers, Laboratoire Ecologie et Biologie des Interactions, quipe Ecologie,
Evolution,Symbiose,Poitiers,Francuska
3
CentreforCartographyofFaunaandFlora,Ljubljana,Slovenija
4
Laboratoire Signalisation et Transports Ioniques Membranaires STIM ERL 7368, Poitiers,
Francuska
Provedenojeopsenoistraivanjepopulacijskogenetikihimorfolokihobiljejabjelonogograka
ujadranskomslijevu.Ovdjeprikazujemorezultatekojiseodnosenaanalizetrijupopulacija(rijeke
Rjeina (RJE), Reka (REK) i Glinica (GLI)) kod kojih je zabiljeen neoekivani genetski uzorak.
Unatosadanjojgeografskojizoliranostitihtrijupopulacija,premarezultatimagenetskihanaliza
mitohondrijskih i mikrosatelitnih biljega, one se svrstavaju u istu grupu. Dobivena genetska
struktura mogla je nastati kao posljedica prenoenja posredovanog ovjekom, ili kao posljedica
prirodnogirenja.Prirodnoirenjejenajvjerojatnijiuzrokdobivenestruktureiznekolikorazloga;
Malojevjerojatnodabisedobiveneumjerenevrijednostioekivaneheterozigotnosti(He=0,34u
GLI, =0,36 uREK,=0,44uRJE)pojavileu novo uspostavljenim populacijama nako nprenoenja
posredovanog ovjekom.Nadalje,povezanostizmeuReke iRjeine jeveotprijeutvrena na
osnovu prouavanja rasprostranjenosti vrsta roda Niphargus. Zakljuno, prema geografskom
poloaju i hidrologiji istraivanih rijeka, prirodno irenje se moglo dogoditi kao posljedica
povezanostiizvorinihdijelovatijekomgeolokeprolostiilizbogirenjarakovakrozpodzemne
vodenesustave.Zadnjumogunosttrebauzetiuobzirjerpostojebrojninalazibjelonogograka,i
njegovesestrinskevrste,potonograka,vrlodubokounutarpiljskihsustava.
th
12.HBK17212 CBC
M.Jeli1,F.Grandjean2,M.Govedi3,G.Klobuar1,D.Guyonnet4,I.Maguire1
1
UniversityofZagreb,FacultyofScience,DepartmentofBiology,DivisionofZoology,Zagreb,
Croatia(misel.jelic@biol.pmf.hr)
2
UniversitdePoitiers,LaboratoireEcologieetBiologiedesInteractions,quipeEcologie,
Evolution,Symbiose,Poitiers,France
3
CentreforCartographyofFaunaandFlora,Ljubljana,Slovenia
4
Laboratoire Signalisation et Transports Ioniques Membranaires STIM ERL 7368, Poitiers,
France
Thecomprehensiveresearchonpopulationgeneticsandmorphologicalcharacteristicsofthe
whiteclawed crayfish from Adriatic Basin was conducted. We present results that include
analysesofthreepopulations(Rjeina(RJE),Reka(REK),andGlinica(GLI)rivers),inwhich
anunexpected geneticpattern was observed.Despitethecurrentgeographicalisolationof
thesethreepopulations,resultsofgeneticanalysesgroupedthemintothesamecluster.The
obtainedgeneticstructurecouldbeaconsequenceofhumanmediatedtranslocations,ora
consequenceofnaturaldispersal.Naturaldispersalisthemostprobablecauseofobtained
structure for several reasons; Observed moderate values of expected heterozygosity (He
=0.34 in GLI, =0.36 in REK, =0.44 in RJE) were not likely to appear in newly established
populations after humanmediated translocations. Further, the connection between the
Reka and Rjeina rivers was recognized based on the distribution of Niphargus species.
Finally, according to geographical position and hydrology of examined rivers, natural
dispersal could appear due to historical headwater river captures or due to dispersal of
crayfish through underground water systems. The last possibility should be taken into
considerationbecauseitisnotunusualtofindthewhiteclawedcrayfishanditscongeneric
species,thestonecrayfish,verydeepinsidecavesystems.
Keywords:EasternAdriaticBasins,Croatia,Slovenia,mitochondrialmarkers,microsatellitemarkers
P33
FILOGENIJA I FILOGEOGRAFIJA RODA Elmis (COLEOPTERA, ELMIDAE) KRKIH TEKUICA
JUGOISTONEEUROPE
L.Jovovi1,B.BruvoMaari1,V.MietiStankovi2,3,M.A.Jch4,M.Kuini3
1
Zavod za molekularnu biologiju, Institut Ruer Bokovi, Bijenika 54, HR10000 Zagreb,
Hrvatska(jovovic.lada@gmail.com)
2
Hrvatskiprirodoslovnimuzej,Zagreb,Hrvatska
3
Bolokiodsjek,Zoologijskizavod,Prirodoslovnomatematikifakultet,Zagreb,Hrvatska
4
PrirodoslovnimuzejuBeu,Vienna,Austria
VodenikornjaiizporodiceElmidaesuslaboistraeninapodrujujugoistoneEuropepaje
malo podataka i o njihovim filogenetskim i filogeografskim odnosima. Tako su za ovo
istraivanje sakupljeni predstavnici navedene porodice u Hrvatskoj, Bosni i Hercegovini i
Makedoniji,doksuoniizAustrijekoritenizausporedbu.Upotrebomstandardnihgenetikih
markera (ITS1, COI i COII) pokazali smo da su vrste Elmis aenea i E. rioloides sestrinske te
zajednonajsrodnijevrstiE.bosnica.Takoer,svesutrinavedenevrsteusrodstvusavrstom
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12.HBK17312 CBC
E.maugetiimaugetii.Sdrugestrane,filogenetskipoloajvrsteE.obscuranijejorazjanjen.
Kod vrsta E. aenea i E. rioloides sakupljenih na rijeci Cetini uoene su morfoloke razlike u
odnosunajedinkeistihvrstausredinjemdijeluEurope.Stogajeglavniciljovogistraivanja
analizirati moe li se uoena morfoloka razlika potvrditi i na molekularnoj razini. Uzorci
vrste E. aenea ne pokazuju znaajne molekularne razlike s obzirom na uoeni morfoloki
dimorfizamilokalitete.AnalizauzorakavrsteE.rioloidessrijekeCetineukazujenaodvajanje
od tipinih predstavnika srednjoeuropske faune podupirui dosadanji status potencijalne
kompleks vrste. Metodom molekularnog datiranja izraunali smo da je do razdvajanja
izmeugrupavrsteE.rioloidesizSrednjeEuropeispodrujaCetinedolouPleistocenuto
jeuskladuspretpostavkomovanostipleistocenskihrefugijauspecijacijskimprocesimana
podrujujugoistoneEurope.
Kljunerijei:vodenikornjai,Elmidae,kompleksvrste,Balkan,pleistocen
PHYLOGENYANDPHYLOGEOGRAPHYOFGENUSElmis(COLEOPTERA,ELMIDAE)INKARSTIC
RUNNINGWATERSINSOUTHEASTERNEUROPE
L.Jovovi1,B.BruvoMaari1,V.MietiStankovi2,3,M.A.Jch4,M.Kuini3
1
Department for Molecular Biology, Ruer Bokovi Institute, Zagreb, Croatia
(jovovic.lada@gmail.com)
2
CroatianNaturalHistoryMuseum,Zagreb,Croatia
3
DepartmentofBiology,DivisionofZoology,FacultyofScience,Zagreb,Croatia
4
MuseumofNaturalHistoryinVienna,Vienna,Austria
ElmidaeisawaterbeetlesfamilypoorlystudiedinsoutheasternEurope,sophylogeneticand
phylogeographic data are very scarce. Representatives of the family were collected in
various freshwater habitats in Croatia, Bosnia and Herzegovina and Macedonia, while
Austrian representatives were used for needed comparisons. Using standard genetic
markers (ITS1, COI and COII) we revealed sister group of Elmis aenea and E. rioloides
together being related to E. bosnica. E. maugetii maugetii forms a separate branch, while
positionofE.obscuracouldnotbeaffirmedwithhighsupport.Inaddition,E.aeneaandE.
rioloides collected in Cetina River showed morphological variations from Central European
representatives.Therefore,themainaimofthisstudywastoanalysewhethertheobserved
differences can be confirmed at the molecular level. E. aenea shows high molecular h
omogeny across all sampled populations in respect to both morphological features and
locations. On contraire, screening of E. rioloides specimens indicated the separation of
population from the Cetina River from typical representatives of Central European fauna,
supportingthehypothesisthatthisisacomplexspecies.Bymoleculardatingwecalculated
thatE.rioloidesseparatedfromCentralEuropeanrepresentativesinthePleistocene.Thisis
consistent with the observation of the importance of Pleistocene refugia in speciation
processesintheSoutheasternEurope.
Keywords:waterbeetles,Elmidae,complexspecies,Balkan,Pleistocene
P34
SIMBIOZAHIDREIALGE:KARAKTERIZACIJAENDOSIMBIOTSKIHZELENIHALGIIZOLIRANIHIZ
RAZLIITIH SOJEVA ZELENIH HIDRI POMOU ELEKTRONSKE MIKROSKOPIJE,
MORFOMETRIJSKEIENZIMSKEANALIZE
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12.HBK17412 CBC
N.Kevi1,S.RadiBrkanac2,P.Peharectefani3,F.Faraguna4,M.Kalafati1,G.Kovaevi1,D.Franjevi1
1
Zoologijski zavod, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska(nives.rajevic@biol.pmf.hr)
2
Botaniki zavod, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska
3
Zavod za molekularnu biologiju, Bioloki odsjek, Prirodoslovnomatematiki fakultet,
SveuiliteuZagrebu,Zagreb,Hrvatska
4
Fakultetkemijskoginenjerstvaitehnologije,SveuiliteuZagrebu,Zagreb,Hrvatska
Procesendosimbiozejedanjeodpokretaaspecijacije.Zelenahidra(HydraviridissimaPallas,
1766) tipianje primjerendosimbioze, jeru svojim gastrodermalnim mioepitelnimstanicama
sadri jednostanine fotoautotrofne alge. Endosimbiotske alge izolirane iz zelenih hidri
odravamoutrajnimstabilnimlaboratorijskimkulturama.Sojevizelenihhidriprikupljenisusa
est razliitih geografskih lokaliteta: s etiri razliite lokacije u Hrvatskoj, te iz Izraela i iz
Njemake. Endosimbiotske zelene alge izolirane su koristei mikrobioloke metode. Izolirane
endosimbiotske alge karakterizirane su prema citolokomorfometrijskim parametrima te
premaaktivnostiisastavuenzimakatalaze,peroksidazeiesteraza.Staniceendosimbiotskihalgi
karakterizirane su morfometrijskim mjerenjem promjera, opsega i povrine. Alge HV i M9
odvojile su se od T i BV u veliini stanica. Rezultati dobiveni analizom sastava izoenzima
upuuju na razliku izmeu algalnih endosimbionata s hrvatskih lokaliteta i algalnih
endosimbionataizEurope,ukazujuinabiolokuraznolikostmeualgalnimsimbiontima.PAGE
elektroforezom izolirano je ukupno pet izoenzima katalaze i pet izoenzima peroksidaze u
endosimbiotskimalgama.IzoenzimK1pojaviosejedinokodendosimbiotskealgeM9izIzraela,
aizoenzimK3jedinokodTsoja.IzoenzimP1pojaviosejedinokodendosimbiontaHV,izoenzim
P5kodHViM9.IzoenzimiP3iP4pojavilisusejedinokodalgiBViTizHrvatske.
Kljunerijei:endosimbiotskealge,zelenahidra,simbioza,morfometrija,izoenzimskaanaliza
Processofendosymbiosisisoneofpromotersofspeciation.Thegreenhydra(Hydraviridissima
Pallas,1766)isatypicalexampleofendosymbiosis.Itsgastrodermalmyoepithelialcellsharbor
the individuals of unicellular photoautotrophic algae. We have established prosperous
laboratory cultures of endosymbiotic green algae isolated from green hydra strains. Hydra
strainswerefrom6differentgeographicallocalities:4fromCroatia,1fromIsraeland1from
Germany.Endosymbioticgreenalgaeweresuccessfullyisolatedusingmicrobiologicalmethods.
Isolated endosymbiotic algae were characterized employing cytological morphometric
parameters, enzyme activity and isoenzyme pattern analysis (catalase, peroxidase and
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esterases).Cellsofendosymbioticalgaewerecharacterizedbymorphometricmeasurements:
diameter,perimeterandarea.AlgalHVandM9strainswereseparatedfromstrainsTandBV.
TheresultsobtainedbyisoenzymepatternanalysissuggestadifferencebetweenCroatianand
European algal endosymbionts, indicating biological diversity among algal symbionts isolated
from green hydras. Five isoenzymes of catalase and five isoenzymes of peroxidase were
resolved by PAGE electrophoresis. Isoenzyme K1 appeared only in endosymbiotic algae M9
from Israel and isoenzyme K3 only in T strain. Isoenzyme P1 was observed only in
endosymbiontHVwhileisoenzymeP5inbothendosymbiontsHVandM9.IsoenzymesP3and
P4werevisibleonlyinalgalstrainsBVandTfromCroatia.
Keywords:endosymbioticalgae,greenhydra,symbiosis,morphometry,isoenzymeanalysis
P35
ErIK:SOFTVERSKAPLATFORMAZAIDENTIFIKACIJUGUJAVICAHRVATSKE
D.K.Hackenberger,B.K.Hackenberger,D.K.Hackenberger
Odjel za biologiju, Sveuilite J. J. Strossmayera u Osijeku, Osijek, Hrvatska
(domagojhack@gmail.com)
Fauna beskraljenjaka u Hrvatskoj nedovoljno je istraena. est razlog neistraenosti je
"neaktraktivnost" svojti, ali i nedovoljna dostupnost kljueva za odreivanje. Osobito su
neistraeni beskraljenjaci tla, kako u Hrvatskoj tako i u Europi to se u posljednje vrijeme
pokuava promijeniti s obzirom na znaaj edafobionata za funkcionalnost i zdravlje ekolokih
sustava.Jednaskupinaedafobionatasugujavice(Lumbricidae),kojihjeza faunuHrvatskedo
sadazabiljeeno68vrstarasporeenihu17rodova.Osobitufaunistikuznaajnostinideset
endemskihvrsta.KakobiseolakalonjihovoodreivanjerazvilismoKljuzaodreivanjevrsta
gujavica Hrvatske ErIKuoblikuraunalnogprograma.Kljujedizajnirantakodase pomou
razliitihalataislikaolakaodreivanjedorazinevrste.Glavniprozorprogramasadriopisne
tekstove (vanjska i unutranja graa, distribucija), kartu pronalaska vrste, crte vrste i popis
svih vrsta u Hrvatskoj. Odreivanje se moe provesti na dva naina pomou tablinog i
pomou dihotomskog kljua. Tablini klju omoguuje izravno upisivanje morfolokih i
anatomskihparametarapromatranejedinke.Moguejeupisatiukupno18parametara,anije
potrebno upisati sve kako bi klju radio. Dihotomski klju temelji se na klasinom kljuu
odreivanjavrstapoprincipudvaizbora.Unutarprogramasenalaziiprirunikzaupotrebus
opisomsvihmorfolokihianatomskihobiljeja.Programjebesplatanidostupanzaskidanjesa
mrenestranice.
Kljunerijei:Lumbricidae,tabliniklju,dihotomskiklju
ErIK:ASOFTWAREPLATFORMFORIDENTIFICATIONOFEARTHWORMSOFCROATIA
D.K.Hackenberger,B.K.Hackenberger,D.K.Hackenberger
Department of Biology, J. J. Strossmayer University in Osijek, Osijek, Croatia
(domagojhack@gmail.com)
12.HBK17612 CBC
beenrecordedforCroatia,ofwhich10endemicspecieshaveaparticularfaunisticsignificance.In
ordertofacilitatetheirdeterminationwehavedevelopedEarthwormIdentificationKeyErIKasa
computer program. The key is designed so that usage of different tools and images facilitates
determination to species level. The main window contains descriptions (anatomy and
morphology, distribution), map with species records, drawing of the species and the list of all
speciesinCroatia.Determinationcanbecarriedoutintwoways,usingtabularanddichotomous
key. The tabular key enables entering morphological and anatomical parameters of observed
individuals. A total of 18 parameters can be inserted, but not all for key to function. The
dichotomouskeyisbasedonaclassicdeterminationkeywithtwochoices.Thereisamanualwith
descriptionofallmorphologicalandanatomicalcharacteristicswithinthesoftware.Thesoftware
isfreeandavailablefordownloadfromawebsite.
Keywords:Lumbricidae,tabularkey,dichotomouskey
P36
FLORAOTOIAOLTANSKOGARHIPELAGA
M.Rui1,M.eput2
1
PrirodoslovnomatematikifakultetSplit,SveuiliteuSplitu,Split(mrus@pmfst.hr)
2
OsnovnakolaCavtat,Cavtat
Istraivana je flora 7 otoia: Stipanska, Balkun, Rudula, Polebrnjak, Grmelj, Saskinja i hrid
Kamik, smjetenih jugozapadno od obala otoka olte. Utvrena je velika raznolikost
vaskularne flore, najvei broj vrsta je zastupljen na otoiu Stipanska i iznosi 141 vrstu.
NajzastupljenijeporodiceuukupnoistraivanojflorisuFabaceae(10,55%),Poaceae(8,69%)i
Asteraceae (5,59%). Najvei broj vrsta i podvrsta flore otoia pripada mediteranskom
flornomelementu(63,96%)itoopemediteranskimbiljkama(64%).Uokvirumediteranskog
elementa veliko znaenje imaju ilirskomediteranske biljke (9,7%) te u sklopu njih ilirsko
jadranske endemine biljke (9,87%). Analizom ivotnih oblika flore otoia utvrena je
najveazastupljenostterofita(30,43%),potomfanerofitas(24,85%)ihemikriptofita(18,63
%).Ufloriotoiautvrenoje5,6%endeminihbiljaka.Istraenesuiugroenesvojte(2%),
zatiene(12%)istrogozatienesvojte(6,8%).
Kljune rijei: flora, Stipanska, Balkun, Rudula, Polebrnjak, Grmelj, Saskinja, Kamik, otoii,
endemi
THEFLORAOFOLTAARCHIPELAGOISLETS
M.Rui1,M.eput2
1
FacultyofSciencesSplit,UniversityofSplit,Split(mrus@pmfst.hr)
2
ElementarySchoolCavtat,Cavtat
We investigated the flora of 7 islands: Stipanska Balkun, Polebrnjak, Grmelj, Saskinja and
rock Kamik, located southwest coast of the island of Solta. There was a great diversity of
vascular flora, the largest number of species is represented on the island Stipanska and is
141 species. The most abundant families in the studied flora of the Fabaceae (10.55%),
Poaceae(8.69%),andtheAsteraceae(5.59%).Thehighestnumberofspeciesandsubspecies
of flora islands belong to the Mediterranean floral element (63.96%) and general
Mediterranean plants (64%). Within the Mediterranean element of great significance have
Illyrian and Mediterranean plants (9.7%) and within them IllyrianAdriatic endemic plants
(9.87%). The analysis of life forms flora islands established a major presence Therophyta
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(30,43%), then Phanerophytes with (24.85%) and Hemikryptophytes (18.63%). The flora of
the islands was found 5.6% of endemic plants. Have been investigated and threatened
species(2%),protected(12%)andstrictlyprotectedspecies(6.8%).
Keywords:flora,Stipanska,Balkun,Rudula,Polebrnjak,Grmelj,Saskinja,Kamik,endems
P37
ANALIZA DIJELA HERBARIJA CARLA STUDNICZKE (ORD. CAPRIFOLIACEEN, LONICEREEN I
STELLATEEN)
N.evrnja,B.Miti2,D.Vladovi
Prirodoslovnimuzejizoo,Split,Hrvatska(nediljko@prirodoslovni.hr)
2
Botanikizavod,PMFSveuilitauZagrebu,Zagreb,Hrvatska
Kljunerijei:PrirodoslovnimuzejSplit,herbarij,Studniczka
N.evrnja,B.Miti2,D.Vladovi
TheNaturalScienceMuseumandZooGarden,Split,Hrvatska(nediljko@prirodoslovni.hr)
2
DivisionofBotany,DepartmentofBiology,FacultyofScience,UniversityofZagreb,Zagreb,
Croatia
Keywords:Studniczka'sherbarium,NaturalHistoryMuseumSplit
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P38
POPULACIJSKA EKOLOGIJA I SPOLNI DIMORFIZAM LIVADNE GUTERICE Lacerta agilis,
LINNAEUS1758SDINAREUHRVATSKOJ
S.Bakiera1,M.Vuci2,D.Jeli1
1
Hrvatski institut za bioloku raznolikost, Hrvatsko herpetoloko drutvo HYLA, Zagreb,
Hrvatska(senka.bx@gmail.com)
2
Sveuilite u Zagrebu, Prirodoslovnomatematiki fakultet, Bioloki odsjek, Zoologijski zavod,
Zagreb,Hrvatska
Livadnaguterica,LacertaagilisLinnaeus,1758jemaliguerkojinaseljavaEuropuiistonidio
Azije,ukljuujuiiveidioHrvatske.Poetkomkolovoza2012.godine,provelismoistraivanje
ove vrste na planini Dinara u Hrvatskoj. Tijekom tog istraivanja smo uhvatili i izmjerili 100
jedinkilivadnegutericekojesmokasnijepodijeliliudvijegrupenatemeljuspola.Jednagrupa
jesadravala56odraslihenkidokjedrugasadravala40odraslihmujaka.Ulovljenesuietiri
juvenilne jedinke koje su izostavljene iz statistike analize. Za svaku je jedinku zabiljeen set
ekolokihistaninihparametarapoulovu.Aprijeputanjajezasvakujedinkuizmjerenamasa
(m)isljedeemorfometrijskekarakteristike:ukupnaduinatijelaenkemjerenaodvrhanjuke
dokrajarepa(Ltot),duinatijelaenkeodvrhanjukedokloake(SVL),duinarepa(Lt),duina
od vrha njuke do kraja slunog otvora (Lhel), duina od vrha njuke do prednjeg uda (Lsfl),
duljinaodprednjegdostranjeguda(Lfhl),visinaglave(HH)iirinaglave(HW).Svesujedinke
ulovljene na visokoplaninskom travnjaku izmeu 1000 i 1400 mnm sa sredinjom
temperaturom stanita od24,9 C. Srednjatjelesna temperaturaguterica bilaje 27,7C bez
statistiki znaajne razlike izmeu dvije grupe. Na temelju analize morfometrijskih podataka,
zabiljeen je spolni dimorfizam u tri kategorije: SVL, Lt i tjelesna masa. Oko 60% ulovljenih
jedinkijeimaloregeneriranreptoupuujenavisokipredatorskipritisak.
Kljunerijei:Lacertaagilis,dimorfizam,morfometrija
S.Bakiera1,M.Vuci2,D.Jeli1
1
Croatian Institute for Biodiversity, Croatian Herpetological Society HYLA, Zagreb, Croatia
(senka.bx@gmail.com)
2
FacultyofScience,DepartmentofBiology,DivisionofZoology,Zagreb,Croatia
Thesandlizard,LacertaagilisLinnaeus,1758isasmalllacertidlizarddistributedacrossEurope
andeasternAsia.SandlizardalsoinhabitsmostofCroatiaandisproclaimedstrictlyprotectedby
CroatianNatureProtectionAct.InAugust2012,weconductedaresearchonDinaraMountainin
Croatia. Dinara is the highest Croatian mountain stretched from Dalmatia over to Bosnia and
Herzegovina.Duringthisresearchwecollectedandmeasuredonehundredspecimens.Lateron,
weputthemintotwogroupsaccordingtosex.Onegroupconsistedof56femalesandotherof40
males. Four juveniles were captured during this research but were excluded from following
statisticalanalysis.Foreachindividualasetofecologicalandhabitatparameterswastakenwithin
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minutes from capture. Before release, body mass (m) and following morphometric characters
weretaken:totalbodylength(Ltot),snouttoventlength(SVL),taillength(Lt),snouttoearend
length(Lhel),snouttoforelimblength(Lsfl),forelimbtohindlimblength(Lfhl),headheight(HH)
andheadwidth(HW).Allindividualswerecollectedonhighmountaingrasslandsbetween1000
and1400ma.s.l.withmeanhabitattemperatureof24.9C.Meanbodytemperaturewas27.7C
with no significant difference between sexes. Morphological data indicate that there is
statistically significant sexual dimorphism in SVL, Lt and body mass. Almost 60% of captured
individualshadregeneratedtails,indicatinghighpredationpressure.
Keywords:Lacertaagilis,dimorphism,morphometry
P39
DOPRINOSPOZNAVANJUHERPETOFAUNEDINAREUHRVATSKOJIBLIEOKOLICE
S.Bakiera1,M.Vuci2,B.Lau1,I.Vilaj1
1
Hrvatski institut za bioloku raznolikost, Hrvatsko herpetoloko drutvo HYLA, Zagreb,
Hrvatska(senka.bx@gmail.com)
2
Sveuilite u Zagrebu, Prirodoslovnomatematiki fakultet, Bioloki odsjek, Zoologijski zavod,
Zagreb,Hrvatska
Kljunerijei:Dinara,vodozemci,gmazovi
CONTRIBUTIONTOTHEKNOWLEDGEOFHERPETOFAUNAOFCROATIANPARTOFDINARA
MOUNTAINANDITSVICINITY
S.Bakiera1,M.Vuci2,B.Lau1,I.Vilaj1
1
Croatian Institute for Biodiversity, Croatian Herpetological Society HYLA, Zagreb, Croatia
(senka.bx@gmail.com)
2
FacultyofScience,DepartmentofBiology,DivisionofZoology,Zagreb,Croatia
DinaraisamountainlocatedinthelargemountainchainDinaridesontheborderbetween
CroatiaandBosniaandHerzegovina.Itismadeofcarbonaterocksandis84kilometerslong.
ItspeakSinjal,alsocalledDinara(1831ma.s.l.),isthehighestmountainpeakinCroatia.Due
to its limited accessibility and mined areas, knowledge of Dinara's flora and fauna is
incomprehensive.BiologyStudentsAssociationBIUScarriedouttwofieldtripstomountain
Dinara, one in May and one in August 2012. Main goal of our research was to make a
quantitative list of herpetofauna of researched parts of Mt. Dinara. Literature data states
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that20speciesofamphibiansandreptilesinhabittheseareas.Ourinventorylistcounts13
reptile and 3 amphibian species (16 in total), including species found on hiking trail to
mountainDinarafromtownKnin.Themostfrequentlyrecordedspecieswasthesandlizard,
Lacerta agilis. The most important finding was Orsini's viper, Vipera ursinii, which is a
regional endemic species of the area, a species that is listed in the Croatian Red Book of
AmphibiansandReptilesandstrictlyprotectedbytheCroatianNatureProtectionAct.
Keywords:Dinara,amphibians,reptiles
P40
IS POPULATION OF MOOR FROG (Rana arvalis) UNDER SEVERE DECLINE IN MURA RIVER
BASININSLOVENIA?
M.Cipot,A.Lenik,K.Poboljaj
1
Center for Cartography of Fauna and Flora, Miklav na Dravskem Polju, Slovenija
(maja.cipot@ckff.si)
Keywords:Moorfrog,Ranaarvalis,Slovenia,riverMura,populationdecline
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12.HBK18112 CBC
P41
HERPETOFAUNAOFPOSAVINAREGIONINBOSNIAANDHERZEGOVINAANDCROATIA
A.uri1,A.Zimi2,D.Jeli3
1
Society for Research and Protection of Biodiversity, Banja Luka, Bosnia and Herzegovina
(anna.curic@hotmail.com)
2
HerpetologicalAssociationinBosniaandHerzegovinaATRA,Sarajevo,BosniaandHerzegovina
3
CroatianInstituteforBiodiversity,CroatianHerpetologicalSocietyHYLA,Zagreb,Croatia
Posavina region received its name from Sava River, which dominates its landscape, and it
incorporatesalargepartofthesurroundinghillyareasandlowlandsoflargetributaryrivers.
Borderareaisstillminepolluted,especiallyonB&Hside,andthereisaseriouslackofdatafor
many species groups, including herpetofauna. During year 2014 and 2015 we collected
significantnewdataforB&HandCroatia,whichwecombinedwithknownliteraturedata,data
from museum collections and local expert personal data in order to present updated list of
Posavina herpetofauna. Field work was done during day and night and all species were
recordedandusuallyphotographed.Wewerestrictlyfocusedonlowlandareas(upto300m
a.s.l.)withtypicalriparianhabitats.InPosavinaregionwefoundtotalof17amphibianand12
reptile species. Specifically for B&H we recorded all together 14 amphibian species (we
highlightPelobatesfuscusandPelophylaxlessonaeasspeciesconfirmedforthefirsttime)and
11reptilespecies(wehighlightLacertaagilisargusandViperaberusbosniensisalsofirsttime
confirmed). One individual of Pelodiscus sinensis, allochthonous species, has been caught on
Bardaain2010andmovedintocaptivity.Croatiahadmuchmorereliableliteraturedataand
combined with new personal data we arrive to the number of 17 amphibian and 11 reptile
species.ThemostinterestingdataforCroatiaarelowlandpopulationsofZootocaviviparaand
Ichtyosauraalpestris.
Keywords:amphibia,reptilia,lowland,distribution,PannonianBasin
P42
EFFECT OF ENVIRONMENTAL TEMPERATURE ON BODY CONDITION AND JUMPING
PERFORMANCEOFTWOGEOGRAPHICALLYDISTANTRanatemporariaPOPULATIONS
S.Drakuli1,H.Feldhaar2,D.Lisii3,M.Mio3,I.Cizelj4,M.Seiler2,T.Spatz5,M.O.Rdel1
1
Museum fr Naturkunde, Leibniz Institute for Evolution and Biodiversity Science, Berlin,
Germany(sanja.drakulic@mfnberlin.de)
2
DepartmentofAnimalEcologyI,UniversityofBayreuth,Bayreuth,Germany
3
DepartmentofAnimalPhysiology,FacultyofScience,UniversityofZagreb,Zagreb,Croatia
4
ZoologicalgardenofZagreb,Zagreb,Croatia
5
Department of Animal Ecology and Tropical Biology, University of Wrzburg, Wrzburg,
Germany
Environmentaltemperaturesaffectallphysiologicalprocessesandperformanceofectotherms.
Performance directly influences fitness by determining the probability of survival. Therefore,
physiologicaladjustmentstolocalthermalenvironmentareextremelyimportantforachieving
optimalperformancelevels.RanatemporariaisawidelydistributedEuropeanfrog,presentin
a variety of habitats. This makes it an exceptional model for studying populationspecific
physiological adjustments to the local environmental conditions. We hypothesized that R.
temporaria populations from colder (Central Europe) and warmer (Southeast Europe)
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Keywords:frog,environmentaltemperature,physiologicaladjustment,climatechange
P43
SPATIAL REPLACEMENT OF CLOSE RELATIVES, A PASSIONATE STORY OF A MOUNTAINOUS
POPULATIONOFViperaammodytes
A.Dyugmedziev,N.Tzankov,G.Popgeorgiev
Vertebrates Department, National Museum of Natural History BAS, Sofia, Bulgaria
(angel_diugmedjiev@abv.bg)
We studied the uppermost part of Struma valley in Western Bulgaria, where the nominate
subspeciesandV.ammodytesmontandonicomeintocontact.Historically,tillthemidnineties
only the nominate subspecies has been found regularly there. In the midnineties, however,
multiple farms for venom went bankrupt and thus an unknown number of specimens were
released outside of their native range; unconfirmed data suggests that hundreds of snakes
wereincidentallyreleasedclosetothestudyarea.WhenasingleV.a.montandoniwasfound
in2008,anincidentalintroductionaswellasapossiblenaturalpenetrationinthestudyarea
wasassumed.Duringthenextyears,intensivestudiesrevealedvirtuallyacompleteexchange
of the nominate subspecies by V. a. montandoni. In order to evaluate the degree of this
process, spatial analyses with Maxent software of the existing locations were carried out
togetherwithsixgeographicandsamenumberclimaticvariables.Theanalysesdemonstrateda
great degree of spatial nicheoverlap (73%) between the two taxa. The additional analysis of
the locations raster values revealed statistically significant difference in two parameters,
namely the potential evapotranspiration, where the nominate ssp. was with higher values,
being more related to places with higher overall humidity, and the altitude, where V. a.
montandoni showed a more narrow altitudinal range. Likely, recent global and local climate
changescontributedtotheobservedprocessthatcouldalsobeanaturaldistributionshift.
Keywords:invasion,ecologicalsubstitution,coocurrence
P44
FAUNISTIKINALAZIVODOZEMACAIGMAZOVAUOKOLICINAICA
D.Hlavati1,M.Zadravec2
1
JosipaJurjaStrossmayera100,Naice,Hrvatska(dhlavati090@gmail.com)
2
HrvatskoherpetolokodrutvoHyla,ZagrebHrvatska
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UnovijevrijemenijebilomnogofanustikihistraivanjaherpetofaunenapodrujuSlavonije.U
ovomraduprikazanisunalaziistraivanjavodozemacaigmazovanaosamlokalitetauokolici
Naicaod 2010. do2014. godine. Vodozemcisuhvatani mreama ilirukom, a gmazovi samo
rukom. Jedinke se fotografiralo i pustilo neozljeene nazad na mjesto ulova. Ukupno je
zabiljeeno devet vrsta vodozemaca i devet vrsta gmazova. Gotovo sve zabiljeene vrste su
oekivane, uz iznimku invazivne crvenouhe kornjae, Trachemys scripta elegans (Wied
Neuwied, 1839). Naena je jedna odrasla jedinka u umjetnom jezeru u gradskom parku u
Naicama, 2010. Vrlo vjerojatno je rije o namjerno putenom kunom ljubimcu. Dvije
zabiljeene vrste, uti muka, Bombina variegata (Linaneus, 1758) i barska kornjaa, Emys
orbicularis (Linnaeus, 1758), koji su na Dodacima II i IV Direktive o stanitima Europske
komisije. Kroz ovo istraivanje prikupljeni su prvi konkretni podaci o prisutnosti vrsta
vodozemaca i gmazova na tom podruju Hrvatske i moe koristiti kao podloga za budua
istraivanja.
Kljunerijei:herpetofauna,Natura2000,Slavonija
FAUNISTICRECORDSOFAMPHIBIANSANDREPTILESINTHENAICEAREA
D.Hlavati1,M.Zadravec2
1
JosipaJurjaStrossmayera100,Naice,Croatia(dhlavati090@gmail.com)
2
CroatianHerpetologicalSocietyHyla,Zagreb,Croatia
Inrecenttimestherehasnotbeenalotoffaunisticresearchoftheherpetofaunaintheareaof
Slavonija. Here we give the list of amphibians and reptiles found on eight localities around
Naice,from2010till2014.Amphibianswerecaughtbynetsorbyhand,whilereptileswere
caught only by hand. Caught individuals were photographed and released unharmed
immediately afterwards at the same place where they had been found. A total of nine
amphibianandninereptilespecieswerefound.Almostallofthespecieshadbeenexpectedfor
the area, except the invasive redeared slider, Trachemys scripta elegans (WiedNeuwied,
1839).OneadultindividualwasfoundintheartificiallakeinthecityparkinNaice,in2010.
Mostlikelyitisanintentionallyintroducedpet.Twoofthespecieswefound,theyellowbellied
toad, Bombina variegata (Linaneus, 1758) and the European pond terrapin, Emys orbicularis
(Linnaeus,1758)areonAnnexesIIandIVoftheEuropeanCommision'sHabitatsDirective.This
researchyieldsthefirstexactrecordsontheoccurenceofamphibiansandreptilesinthispart
ofCroatiaandcanbeusedasabasisforfuturework.
Keywords:herpetofauna,Natura2000,Slavonia
P45
MOLEKULARNA FILOGENIJA IVANJSKOG ROVAA (Ablepharus kitaibelii) NA SREDINJEM I
SJEVERNOMDIJELUPODRUJARASPROSTRANJENOSTI
D.Jeli1,M.Jeli2,M.Futo3,B.Halpern4,K.Ljubisavljevi5,6,N.Tzankov7
1
Hrvatski institut za bioloku raznolikost, Hrvatsko drutvo za bioloka istraivanja, Zagreb,
Hrvatska(jelic.dusan@gmail.com)
2
Sveuilite u Zagrebu, Prirodoslovnomatematiki fakultet, Bioloki odsjek, Zoologijski zavod,
Zagreb,Hrvatska
3
University of Mnster, Institute for Evolution and Biodiversity, Animal Evolutionary Ecology
Group,Mnster,Njemaka
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MMEBirdLifeHungary,Budimpeta,Maarska
UniverzitetuBeogradu,InstitutzabiolokaistraivanjaSiniaStankovi,Beograd,Srbija
6
Crnogorskodrutvoekologa,Podgorica,CrnaGora
7
NaturalMuseumofNaturalHistory,VertebratesDepartment,Sofija,Bugarska
Ivanjski rova je termofilna vrsta gutera i spada meu najmanje gmazove Europe.
Rasprostranjen je od Slovake, prema jugu Europe sve do Turske. U Hrvatskoj je nedavno
potvrena njegova prisutnost na obroncima Papuka i u gradu Iloku, to ini sjeverozapadnu
granicu rasprostranjenosti. Do sada su koritenjem mitohondrijskih molekularnih biljega (16S
rRNA i citokrom b) istraivani filogenetski odnosi populacija ivanjskog rovaa u junom dijelu
areala.Zakljuenojedaivanjskirovanijemonofiletskaskupina,vekompleksvrsta.Ciljovog
istraivanjajedasekoritenjemmitohondrijskihbiljega,istraepopulacijenaostatkupodruja
rasprostranjenostiuEuropi,kakobiseupotpunilizakljuciofilogeniji.Zabiljeenihaplotipoviiz
rumunjskihpopulacija,odvojilisusekaonovazasebnalinijanafilogenetskomstablu.Meutim,
genetskavarijabilnostpopulacijaspodrujaHrvatske,Srbije,BugarskeiMakedonijejeizrazito
niska.Nacijelomtompodruju,zabiljeenajeprisutnostsamojednoghaplotipana16SrRNAi
citokrombbiljegu.HaplotipovisuistovjetnionimaizkontinentalneGrke.Tajrezultatukazuje
nanaglo irenje podrujarasprostranjenosti, najvjerojatnije izizvorinepopulacije s podruja
Grke.Naahipotezajedasenagloirenjezbilounajtoplijemrazdobljuzadnjeginterglacijala
kojeodgovararazdobljuatlantika(prije8000do5000godina).
Kljune rijei: termofilna vrsta gutera, Balkanski poluotok, pleistocenska pribjeita, brzo
irenjeareala,holocen
MOLECULARPHYLOGENYOFTHESNAKEEYEDSKINK(Ablepharuskitaibelii)ONTHECENTRAL
ANDNORTHPARTOFITSDISTRIBUTIONRANGE
D.Jeli1,M.Jeli2,M.Futo3,B.Halpern4,K.Ljubisavljevi5,6,N.Tzankov7
1
Croatian Institute for Biodiversity, Croatian Biological Research Society, Zagreb, Croatia
(jelic.dusan@gmail.com)
2
UniversityofZagreb,FacultyofScience, DivisionofBiology,DepartmentofZoology,Zagreb,
Croatia
3
University of Mnster, Institute for Evolution and Biodiversity, Animal Evolutionary Ecology
Group,Mnster,Germany
4
MMEBirdLifeHungary,Budapest,Hungary
5
University of Belgrade, Institute for Biological Research Sinia Stankovi, Belgrade, Serbia
6
MontenegrinEcologistsSociety,Podgorica,Montenegro
7
NaturalMuseumofNaturalHistory,VertebratesDepartment,Sofia,Bulgaria
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same haplotypes were reported for the Central Greece. This result indicates a rapid range
expansion,fromancestralpopulationmostprobablysituatedinGreece.Beingathermophilous
species,wesupposethattheSnakeeyedSkinksexpansionhappenedinthewarmestperiods
duringtheHolocene,correspondingtotheAtlanticperiod(80005000yearsBP).
Keywords:thermophilouslizardspecies,BalkanPeninsula,Pleistocenerefugium,rapidrange
expansion,Holocene
P46
NOVI DOKAZI O POSTOJANJU TROGLOKSENIH PRILAGODBI HERPETOFAUNE DINARSKOG
KRA
K.Koller1,2,P.KonradKova1,2
1
HrvatskoherpetolokodrutvoHYLA,Zagreb,Hrvatska(katarina.koller@gmail.com)
2
Hrvatskiinstitutzabiolokuraznolikost,Zagreb,Hrvatska
UlazakvodozemacaupodzemljeDinarskogkradosadanijedetaljnijepromatran.Uliteraturnim
podacima su do sada zabiljeeni sluajni ulasci vrstau podzemlje, ali njima se nije pridavala
posebna panja.U ovom radu pruali smo vie dokaza o ovom fenomenu i dali smo osnovan
pregled literaturnih podataka. 14. i 15. travnja 2015. godine organizirano je speleoronilako
istraivanje Pazinske jame, s glavnim ciljem pronalaska naeg krkog endema ovjeje ribice
(Proteusanguinus).PazinskajamajesmjetenauPazinu(Istra,Hrvatska)(N5416357;E5011182,
185mn.v.).TokrijekePazinicejedug16,5kmteponireuPazinskujamu.Nakonulazaslijedisto
metara dug kanal koji se proiruje u dvoranu (80 * 20 m) s velikim podzemnim jezerom
(Martelovo jezero). Nakon detaljnog pregleda jezera, sifona i kanala, ovjeja ribica nije
pronaena, ali su pronaene 20 jedinki lombardijske smee abe (Rana latastei), dvije jedinke
smeekrastae(Bufobufo)tejednajedinkarijenekornjae(Emysorbicularis).Svetrivrstesu
zamijeene u najudaljenijem suhom dijelu jame. Vrste su uhvaene, fotografirane i potom
putene.OvojeprvinalazlombardijskesmeeabeirijenekornjaeupiljiuHrvatskoj,itona
mjestugdjejeprisutnapotpunatama.DuplaeMartelovogjezerazabiljeenesuinakupinejaja
smee krastae. Nije primjeeno da su vrste pothranjene. Uoeno je da je vanjska obojanost
smeekrastaebilanetosvijetlijaoduobiajene.BuduidajeuPazinskojjamizabiljeenovako
velikibrojvodozemaca,tedapostojeidrugeindikacijeoprisutnostivodozemacaupodzemlju,
naahipotezajedabiovevrstemoglebitinesamostigoksene,vemodaistigofilnevrste.
Kljunerijei:podzemlje,vodozemci,Ranalatastei,Pazinskajama,dinarskikr
K.Koller1,2,P.KonradKova1,2
1
CroatianHerpetologicalSocietyHYLA,Zagreb,Croatia(katarina.koller@gmail.com)
2
CroatianInstituteforBiodiversity,Zagreb,Croatia
EnteringofamphibiansintotheundergroundofDinaricKarsthasreceivedverylittleattentionin
publishedliterature.Thereisonlyliteraturedataabouttheaccidentalaccessofamphibiansinto
the underground, but without any special overview. Here we provide more evidence on this
phenomenonandgivebasicoverviewofpreviousrecords.Duringthe14thand15thofApril2015
wemadespeleologicalexplorationofPazinskacavewiththemainpurposeofprospectingforthe
Proteusanguinus.EnteranceofPazinskacaveislocatedinPazin(Istria,Croatia)atabout185m
a.s.l..WatercourseofriverPazinicais16.5kmlongandplungesintoPazinskacave.Behindthe
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entrancethereisahundredmeterlongchannelwhichexpandsintothehall(80*20m)witha
large cave lake (Martel Lake). After detailed research of the lake, siphons and channels, the
Proteushasnotbeenfound,butwemadeobservationof20individualsofItalianagilefrog(Rana
latastei),twoindividualsofCommontoad(Bufobufo)andoneindividualofEuropeanpondturtle
(Emysorbicularis).Allofthesethreespecieswereobservedinthedeepestdrypartofthecave.
The specimens were caught, photographed and released.This is the first finding of Italian agile
frogandpondturtleinCroatiainsidethecave,wherecompletedarknessispresented.Alongthe
sandyedgeoftheundergroundlakeastripesofBufobufoeggswerealsonoticed.Therewasno
indicationofstarvationinspecies.ItwasobservedthattheexternalcolorationoftheBufobufo
specimentswassomewhatbrighterthanusual.Becauseofsuchabignumberofamphibiansin
Pazinska cave and because of the other indications about the presence of this species in the
caves,ourhypothesisisthatthesespeciesmaybenotonlystygoxene,butmaybealsostygophile.
Keywords:underground,amphibians,Ranalatastei,Pazinskacave,DinaricKarst
P47
COASTAL POPULATION OF DICE SNAKES (Natrix tessellata) HELPS CLARIFY THE EVOLUTION
OFEURYHALINEPHYSIOLOGYINMARINETETRAPODS
Y.Kornilev1,F.Brischoux2
1
National Museum of Natural History, Bulgarian Academy of Sciences, Sofia, Bulgaria
(yukornilev@gmail.com)
2
CentredEtudesBiologiquesdeChiz,VilliersenBois,France
Thewidespreadrelationshipbetweensaltexcretingstructures(e.g.saltglands)andmarinelife
stronglysuggeststhattheabilitytoregulatesaltbalancehasbeencrucialduringthetransition
to marine life in tetrapods. However, lacking data from intermediate stages (species lacking
salt glands but occasionally using saline environments) preclude to draw a comprehensive
picture of the evolution of euryhaline physiology in these organisms. Populations of Dice
snakes(Natrixtessellata)foraginginthemarineenvironmentalongthecoastoftheBlacksea
provide a unique opportunity to study physiological adaptations to marine life in a marine
snake prototype lacking salt glands. We show that coastal freeranging Dice snakes display
highly variable natremia (plasma sodium concentration) ranging from normonatremia (~145
mmolL1)uptostronghypernatremia(i.e.>195mmolL1).Ourlaboratoryexperimentsindicate
thatthisspeciescansustainlifeinfullstrengthseawater(~35gofsaltperL)uptooneweek
withnovisiblelongtermdamage,butthatsuchtreatmententailedamarkedhypernatremia
thatcanberegulatedonlytroughaccesstofreshwater.Takentogethertheseresultssuggest
that hypernatremic Dice snakes require regular access to freshwater in order to periodically
restore osmotic balance. Our results further suggest that the development of a strong
physiological tolerance toward deviations of the osmotic balance (e.g., increased plasma
sodium)hasbeenacriticalinnovationintheevolutionofaeuryhalinephysiologyandmaywell
haveprecededtheevolutionofsaltglands.
Keywords:salinity,natremia,marinelife
P48
APRELIMINARYSTUDYONTHEBIOACTIVITYOFTHEMICROBIOTAINTHEPOSTCAECALGITRACTOF
THREETestudoSPECIES(TESTUDINIDAE,TESTUDINES)WITHEMPHASISONLACTICACIDBACTERIA
Y.Kornilev1,S.Etem2,N.Natchev2,3,I.Ivanchev4,N.Tzankov1,T.IgnatovaIvanova2
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12.HBK18712 CBC
WeinvestigatedthemicrobiotainhabitingthecolonofTestudograeca,T.marginataandT.
hermanni. We sampledfragments offresh fecesobtainedfromspecimenskeptinarescue
centre. Fecal samples were stored in sterile tubes, chilled to 6 C and transported to the
laboratory in cooling bags. Samples were incubated for 24 hours at 37 C. The delivered
inoculum (100 l) was filtered over a sterile media MRS agar with staining agent Xgal
dissolved in 20 ml DMSO (dimethyl sulfoxide). Applying this specific staining, we
identifiedLactobacillusspecies. Out of three samples we isolated pure cultures that were
thenstainedbytheGrammethod.Asexpected,thecultureswereallGrampositive.Wewill
performDNAandbiochemicalanalysistoidentifytheexactbacterialspecies.Inaddition,we
conducted investigation on the antimicrobial and antifungal activities of the isolated
bacterialstrains(colonies)onelevenpathogenspecies.Wecomparedthebioactivityofthe
colonies to those of two broadspectrum antibiotics Sefpotect and Chloronitromycin (250
mg/ml).Ourresultsindicatethattheinvestigatedtortoisepossessmicroflorawhichprotects
themfromavarietyofgastroentericpathogens.
Keywords:tortoise,gastroentericbacteria,pathogens
P49
MORFOLOKEZNAAJKEAREPOLJARICE,Hierophisgemonensis(1768,Laurenti)
P.Lisiar,B.Lau,I.Vilaj,D.Jeli
Hrvatskiinstitutzabiolokuraznolikost,HrvatskoherpetolokodrutvoHYLA,Zagreb,Hrvatska
(petra.lisicar@outlook.com)
Makar se ara poljarica smatra jednom od najeih vrsta hrvatskog primorja i otoka dosadanji
radovineobuhvaajunjenumorfologijuirasprostranjenost.Kakobiodredilitonudistribucijuvrste
Hierophis gemonensis u Hrvatskoj koristili smo bazu podataka HHD Hyla sa potvrenim GPS
koordinatama.Ciljjetakoerbioanaliziratikarakteristikeimorfolokeznaajkevrste.Istraivanjeje
provedeno na uzorku od 50 jedinki sauvanih u 70%tnom etanolu i 51 ivih jedinki premjerenih
tijekom terenskih istraivanja. Za svaku jedinku su zabiljeena 3 meristika i 14 morfometrijskih
obiljeja.enkesuuglavnommanjeilakeodmujaka.Spolnidimorfizamjepotvrenuduinirepa
te broju trbunih i podrepnih ljuski. Rezultati ukazuju na znaajne razlike u morfometrijskim
znaajkamaizmeukopnenihiotonihpopulacijavrsteH.gemonensis.
Kljunerijei:morfolokerazlike,spolnidimorfizam,meristikeznaajke,Hrvatska
P.Lisiar,B.Lau,I.Vilaj,D.Jeli
Croatian Institute for Biodiversity, Croatian Herpetological Society HYLA, Zagreb, Croatia
(petra.lisicar@outlook.com)
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AlthoughBalkanwhipsnakeisconsideredoneofthemostcommonsnakesofcostalCroatia
and islands, there are no studies about morphological differences and distribution of
Hierophisgemonensistothisday.TodetermineexactdistributionofH.gemonensisweused
HHD Hyla database of confirmed finding sites with GPS coordinates.Our goal was also to
analyse characteristics of species morphology. The study was carried out on 50 specimens
preservedin70%ethanoland51livingspecimensmeasuredduringfieldstudies.Specimens
wereexaminedfor3meristicand14morphometriccharacters.Femalesshowedtendencyto
besmallerandlighterthanmales.Sexualdimorphismisconfirmedintaillength,andnumber
of ventral and subcaudal scales. Results indicate that there is significant difference in
morphometriccharacteristicsbetweeninsularandlandpopulationofBalkanwhipsnake.
Keywords:morphologicaldifferences,sexualdimorphism,meristic,Croatia
P50
PRAENJEZDRAVSTVENOGSTANJAOVJEJERIBICE(Proteusanguinus)UZATOENITVU
UsklopuprojektazatiteovjejeribiceuHrvatskojobavljajuseiinsituistraivanjaivotinja
uzatoenitvu.Buduidanemapodatakaofiziolokojmikroflori,niomikroorganizmimakoji
mogunaruitizdravstvenostanjeovjejeribice,svrhajeovogistraivanjasteitodetaljniji
uvid u zdravstveno stanje ivotinja, razluiti fizioloku mikrofloru od oportunistikih
mikroorganizama i pravih patogena, te u sluaju potrebe provesti ispravnu terapiju.
Obavljajusesljedeatestiranja:(1)Standardnamikrobiologijaobrisciusneupljine,kloake
i koe; (2) Parazitoloka testiranja izmet, strugotine crijeva i organi uginulih ivotinja
nativnipreparatiiotisci;(3)TestiranjenaBatrachochytriumdendrobatidis(BD)inaranavirus
obrisci koe metodom Real Time PCR; (4) Testiranje na Chlamydia sp. obrisci usne
upljine i kloake metodom Real time PCR; (5) Razudba i patohistoloka pretraga uginulih
ivotinja da se dobije to vie podataka o anatomiji i funkciji pojedinih organa. Dosad je
naenveibrojmikroorganizamatla,poputAeromonashydrophilaiAspergillusflavus,kojiu
nepovoljnim uvjetima mogu uzrokovati bolesti vodozemaca, no naene su i bakterije koje
sudjeluju u sprjeavanju zaraza vodozemaca BDom poput Janchtinobacterium lividum.
Parazitolokom pretragom izmeta naeni su samo pseudoparaziti iz vode i vlanog tla. Sve
dosadtestiraneivotinjebilesunegativnenaBD,ranavirusibakterijeChlamydiasp.
Kljunerijei:ovjejaribica,zdravstvenostanje,mikroorganizmi,RealtimePCR
MONITORINGOFHEALTHSTATUSOFTHEOLM(Proteusanguinus)INCAPTIVITY
M.Luka1,E.PruknerRadovi1,D.Horvatek1,I.Cizelj2,F.Martinkovi3,D.Jeli4,.Gottstein1
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12.HBK18912 CBC
Within the conservation of Proteus anguinus (the olm) project in Croatia, besides the field
observations, in situ investigation of the species is also carried out.Since there is a lack of
information on physiological flora and microorganisms which can compromise the olms
health,theaimoftheveterinarypartoftheprojectistolearnasmuchaspossibleaboutthe
health status of these animals. In order to distinguish between physiological microflora,
opportunistic and true pathogens, and to provide the right treatment, if necessary, the
followings tests are performed:(1) Standard microbiology of oral cavity, cloacal and skin
swabs;(2)Parasitologicaltestsoffeces,intestinalscrapingsandorgansofdeadspecimens
nativepreparationsandprints;(3)Batrachochytriumdendrobatidis(BD)andRanavirustests
ofskinswabsbytheRealTimePCRmethod;(4)Chlamydiasp.testoforalcavityandcloacal
swabsbytheRealtimePCRmethod;(5)Autopsyandpathohistologyofdeadspecimens,to
getmoreinformationonvariousorgananatomyandfunction.Sofar,alargernumberofsoil
microorganisms were identified, such as Aeromonas hydrophila and Aspergillus flavus,
potential causes of disease in amphibians under suboptimal conditions. Bacteria species,
suchasJanchtinobacteriumlividum,whichcouldparticipateinpreventionofBDinfectionin
certain amphibian species were also identified. Parasitological tests of fecal samples
revealedthepresenceofpseudoparasitesfromwater.Alltheanimalsanalyzedsofarhave
beennegativeforBD,RanavirusandChlamydiasp.
Keywords:Olm,healthstatus,microorganisms,RealtimePCR
P51
ISOENZYME PROFILES OF SUPEROXIDE DISMUTASE AND CATALASE IN LIVER AND MUSCLE
SELECTEDANURANSPECIES
N.Luki,J.Frii,G.ukalo,R.Deki,B.KukavicaJovanovi
Faculty of Science and Mathematics, University Banja Luka, Banja Luka, Bosnia and
Herzegovina(nata.zagorice@hotmail.com)
Reactiveoxygenspecies(ROS)maycausecelldamageandcontributetochangesincellular
function. Superoxide dismutase (SOD) and catalase (CAT) represent key enzymes of cell
antioxidative metabolism. This study focuses on first comparative analysis of CAT and SOD
isoenzyme profiles found in liver and muscle of following anuran species: Rana graeca,
BombinavariegataandPelophylaxkl.esculentus.Animalswerecollectedatseverallocalities
in Bosnia and Herzegovina. SOD and CAT isoforms were identified using native
polyacrylamideelectrophoresis(PAGE).Nativeelectrophoresisresolvedthepresenceofone
CATisoforminliverofallthreefrogspecies,outofwhichthehighestactivitywasobserved
inspeciesPelophylaxkl.esculentus.Inthemuscle,CATisoformwasdetectedonlyinspecies
Bombina variegata. Comparative analysis of liver and muscle SOD profiles showed gender
andspeciescorrelateddifferences.Obtainedresultsarediscussedintermsofdifferencesin
the environments which these species inhabit in correlation with the antioxidative
metabolism.
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Keywords:antioxidativemetabolism,Ranagraeca,Bombinavariegata,Pelophylaxkl.esculentus
P52
ZMIJEUHERPETOLOKOJZBIRCIPRIRODOSLOVNOGMUZEJAUSPLITU,HRVATSKA
S.Mekini1,3,N.evrnja2,J.Boban2,G.Piasevoli1,D.Vladovi2
1
JavnaustanovazaupravljanjezatienimdijelovimaprirodenapodrujuSplitskodalmatinske
upanije"Moreikr",Split,Hrvatska(smgata@yahoo.com)
2
Prirodoslovnimuzejizoolokivrt,Split,Hrvatska
3
Gata73,Gata,Hrvatska
Kljunerijei:zmije,herpetolokazbirka,prirodoslovnimuzejuSplitu,Hrvatska
S.Mekini1,3,N.evrnja2,J.Boban2,G.Piasevoli1,D.Vladovi2
1
PublicInstitutionfortheManagementofProtectedAreasintheCountyofSplitandDalmatia
Seaandkarst,Split,Croatia(smgata@yahoo.com)
2
NaturalHistoryMuseumandZoo,Split,Croatia
3
Gata73,21253Gata,Croatia
AnalysisoftheherpetologicalcollectionofNaturalHistoryMuseuminSplitfoundthatsnakes
wererepresentedintheherpetologicalcollectionby183inventorynumberswith224samples.
Intheherpetologicalcollectiontherearesamplesof4families,5subfamilies,11genusand15
snakesspecies.ThemostrepresentedsamplesarefromNatracinaeandViperidaefamily,infact
gendersNatrixandVipera.ThelargestnumberofsampleswerecollectedbyGiromettaU.and
CvitaniA.collectingattheareaodDalmatia.Alargepartofthecollectiondoesnotcontain
informationofcollectororcollectiondate.Thecollectionwascreatedinperiodof20iesofXX.
centurytotheendof2014.
Keywords:snakes,herpetologicalcollection,naturalhistorymuseuminSplit,Croatia
P53
DISCOVERYOFANAMPHIKINETICAMPHIBIANSKULLANDITSLINKAGETONEOTENICLIFESTYLE
N.Natchev1,2,S.Handschuh3,S.Lukanov4,N.Tzankov5,B.Naumov4,I.Werneburg6
1
DepartmentofIntegrativeZoology,ViennaUniversity,Vienna,Austria
2
FacultyofNaturalScience,ShumenUniversity,Shumen,Bulgaria
3
VetCoreFacilityforResearch,UniversityofVeterinaryMedicineVienna,Vienna,Austria
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12.HBK19112 CBC
Amongurodelesthereisasignificantvariationinthedegreeofskullkinetics,withsomespecies
possessingahighlykineticskullduringtheirlarvalstages,whichthenossifiesinthecourseofthe
ontogenetic development.Weused highspeedcinematography(420fps) andmicrocomputed
tomography (CT) to study the feeding behaviour in the recently described Bureschs newt
TriturusivanbureschiArntzen&Wielstra,2013.Inadditiontothetypicalamphibianarticulation
between skull roof and palatoquadrate associated structures (pleurokinesis) there were also
flexibleconnectionsbetweenparietalsandfrontals(mesokinesis)andbetweenparietalsandthe
exocipitals(metakinesis).Thismorphologicalpatternissimilartothatobservedinmodernlizards
butthecombinationofpleuro,meta,andmesokineticskullarticulationsfoundintheBureschs
newt represents a new kind of amphikinetics not described for any vertebrate to date. The
functionalanalysisofthecranialkinesisinT.ivanbureschiindicatesthattheadvantageofhaving
lessrigidskullmorphologyistooptimizeforjawclosingmechanism.Weproposethatthecranial
kinesisinT.ivanbureschiincreasestheefficiencyoffastjawclosureandmayrepresentaneotenic
featureretainedfromthenewtlarvae.Thepresenceofintracranialmobilitymaybeinfluencedby
specific(yetundetermined)environmentalconditions.
Keywords:Urodela,kinetics,metamorphosis,adaptation,biomechanics.
P54
"ZAMKESMRTI"NEPRIKLADNIGRAEVINSKIZAHVATIUZATIENIMPODRUJIMAMOGU
UZROKOVATIVISOKUSMRTNOSTVODOZEMACAIGMAZOVA
V.olti1,O.Jovanovi2,D.Jeli3
1
Udrugastudenatabiologije"ZOA",Osijek,Hrvatska(soltic.vito@gmail.com)
2
Odjelzabiologiju,SveuiliteJosipaJurjaStrossmayera,Osijek,Hrvatska
3
Hrvatskiinstitutzabiolokuraznolikost,Hrvatskoherpetolokodrutvo,Zagreb,Hrvatska
12.HBK19212 CBC
Kljunerijei:vodozemci,gmazovi,smrtnost,zamkasmrti,graevinarstvo,parkiralite
"DEATHTRAPS"INAPPROPRIATECONSTRUCTIONSINPROTECTEDAREASCANCAUSEHIGH
MORTALITYINAMPHIBIANSANDREPTILES
V.olti1,O.Jovanovi2,D.Jeli3
1
BiologyStudentAssociation"ZOA",Osijek,Croatia(soltic.vito@gmail.com)
2
DepartmentofBiology,JosipJurajStrossmayerUniversityofOsijek,Osijek,Croatia
3
CroatianInstituteforBiodiversity,CroatianHerpetologicalSociety,Zagreb,Croatia
Amphibians and reptiles are among the most threatened groups of animals on our planet.
Their future survival is directly connected to their ability to adapt to ever spreading
anthropogenichabitats.Butitisalsoimportantthatwemakeourimpacttobeasecologically
friendly as possible. Here we describe the problem of urban constructions which cause very
highanimalmortalitywhenusedinnaturalhabitatscalled"deathtraps".Ourresearchwas
focused on parking drainage system built in front of Kopakirit Nature Park visitor centre in
Kopaevo, Croatia. This construction is made out of around 80 meters of collection channels
andalargeundergroundtank(volumeofaround15m3).Collectionchannelsactaspitfalltraps
collectinganimalsfromaroundparkingsurfaceandfocusingthemtoundergroundtank.This
effect was first observed in May 2010 when more than 200 animals were found within the
underground tank (around 50% of animals were already dead). In 2015 we started a
continuousmonitoringonthislocationinordertoquantifythenegativeeffectandmortality.In
total we collected 245 individuals, belonging to six amphibian and one reptile species, from
which around 5% was already found dead. All animals were removed from the tank during
eachresearchoccasionandliveindividualswerereleasedbackintonature.Ifnotremovedall
animals would die eventually. During April and May sampling we calculated that in average
4.98individualsfallintothetankeachday.Wegiveasetofconservationmeasuresthatcan
reduce the mortality and suggest general guidelines for development of more ecofriendly
constructions.
Keywords:Amphibian,reptile,mortality,construction,deathtrap,carparking
P55
SEXUAL DIMORPHISM, DIET AND REPRODUCTION OF THE GRASS SNAKE (Natrix natrix) IN
THE REGION OF THE MARSHYPOND ECOSYSTEM BARDAA (REPUBLIC OF SRPSKA, BOSNIA
ANDHERZEGOVINA)
G.ukalo1,S.orevi3,Lj.Tomovi2,3
1
Faculty of Sciences, University of Banja Luka, Banja Luka, Republic of Srpska, Bosnia and
Herzegovina(sukalogoran@yahoo.com)
2
InstituteforBiologicalResearchSiniaStankovi,UniversityofBelgrade,Belgrade,Serbia
3
InstituteofZoology,FacultyofBiology,UniversityofBelgrade,Belgrade,Serbia
Inthispublicationwepresentthefirstdataconcerningecologicalcharacteristicsofthegrass
snake, Natrix natrix, based on the data collected during the capturemarkrecapture study in
theareaofmarshypondecosystemBardaabetween2011and2014.Wecaptured,processed
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and marked 172 adult individuals (96 : 76 ), in which we analysed body weight, seven
morphometric characteristics and two meristic traits. Females had larger average values of
bodyweightandofallanalysedmorphometrictraits;however,consideredasaratiooftotal
body length, males had longer tails than females (22.3% compared to 19.3%). Additionally,
maleshadsignificantlylargernumbersofventralandsubcaudalscales.Theanalysisofdietof
the snakes we processed was conducted on the contents spontaneously regurgitated by the
animals,whilebypalpatingabdomensofgravidfemales,weassessedthenumbersofgrowing
follicles/eggs theyhad. Wefound statisticallysignificant differences in the typeofconsumed
preyamongdifferentyears,whichindicatesthegrasssnakesdietaryplasticity.Wealsofound
differencesinthedietsofthetwogenders,wherefemalesmoreoftenconsumedadultgreen
frogs(Pelophylaxsp.),whilemalesusuallyconsumedfish(especiallyPseudorasboraparva).The
smallest female with growing follicles had standard body length (SVL) of 62.6 cm, while the
smallestmalewhichwasobservedinmatinghadaSVLof48.5cm.Clutchsizevariedbetween
8and28.
Keywords:grasssnake,bodysize,diet,clutchsize
P56
DETECTIONOFCONTINUEDANNUALMORTALITYINTHEVULNERABLEALPINESALAMANDER,
Salamandra atra prenjensis (MIKI, 1969) NOT ASSOCIATED WITH THE PRESENCE OF
KNOWNAMPHIBIANPATHOGENS
E.unje1,2,F.Pasmans3,Z.Maksimovi4,A.Martel3,M.Rifatbefovi4
1
Faculty of Natural Science Sarajevo, Sarajevo, Bosnia and Herzegovina
(sunje.emina@gmail.com)
2
HerpetologicalAssociationofBosniaandHerzegovina:ATRA(BHHU:ATRA),Sarajevo,Bosnia
andHerzegovina
3
Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine,
GhentUniversity,Ghent,Belgium
4
DepartmentofMicrobiology,FacultyofVeterinaryMedicine,UniversityofSarajevo,Sarajevo,
BosniaandHerzegovina
The emerging disease, chytridiomycosis plays a key role in amphibian declines and species
extinctions. Additional agents contributing to this issue are Rana virus (Rv) and a recently
discovered fungus: Batrachochytridium salamandrivorans (Bs). Chytridiomycosiss etiological
agent, the fungus Batrachochytrium dendrobatidis (Bd), has been reported on all continents
inhabitedbyamphibians.InEuropeitisdetectedin17EUMemberstates.Occurrenceofthe
disease in Balkan area is almost unknown. In this study we report first results for
chytridiomycosisanalyzesinBosniaandHerzegovina(B&H)forcaudateendemicSalamandra
atra prenjensis. During summer 2013, fieldwork was performed on two B&H Mountains. In
total,145individualsofS.a.prenjensiswereswabbedandscreenedforBdinfection.Analyzes
were performed using quantitative realtime PCR (qPCR). Results were negative for all
individuals. On Mt. Prenj, sick and dead individuals expressing specific symptoms (abnormal
posture, cachexia) were found and collected. Besides for Bd, dead animals were additionally
screenedforBsandRv,whichshowedabsenceoftheseinfections.Additionalhistopathology
of skin also proved absence of chytridiomycosis. The phenomenon has been regularly
registered during several years of fieldwork (2% incidence). Possible explanations of its
occurrencearepresentedinthispaper.
th
12.HBK19412 CBC
Keywords:amphibiandisease,BosniaandHerzegovina(B&H),mountainecosystem
P57
PRELIMINARY ASSESSMENT OF THE LACERTID SKULL MODULARITY A CASE STUDY OF
Podarcismuralis
A.Uroevi1,K.Ljubisavljevi1,A.Ivanovi2
1
UniversityofBelgrade,InstituteforBiologicalResearchSiniaStankovi,Belgrade,Serbia
(aurosevic@ibiss.bg.ac.rs)
2
InstituteofZoology,FacultyofBiology,UniversityofBelgrade,Belgrade,Serbia
Theconceptsofmodularityandmorphologicalintegrationhavegainedprominentattentionin
theevolutionarybiologyinrecentyears.Herewepresentastudyofdorsalandventralcranium
modularitywithinthesinglespecies Europeanwalllizard (Podarcismuralis Laurenti,1768),
onasampleof25neonates,26femalesand26males.Forthedorsalcranium,weevaluated
three hypotheses which were already suggested for lizard dorsal cranium: mammalian
hypothesis, Anolis hypothesis and tripartite hypothesis. For the ventral cranium, we tested
two hypotheses: the developmental hypothesis (braincase vs. dermatocranium) and the
functional hypothesis (jaw and palate vs. posterior cranium). Since the allometry was
significant in the whole sample, we did our testsof modularity hypotheses on sizecorrected
dataset.ForthedorsalcraniumonlyAnolishypothesiswassupportedforadultfemalesand
males,butnotfortheneonates.Fortheventralcranium,onlythefunctionalhypothesiswas
supportedforadultfemalesandmales,butnotforneonates.Ourconclusionswouldbethat
there is an onthogenetic shift in the pattern of skull integration, from neonates which have
highly integrated skull, to adults in which functional constraints lead to distinct functional
modularity.
Key words: cranium modularity, developmental hypothesis, European wall lizard, functional
hypothesis
P58
RIOVKA,Viperaberus(SQUAMATA,VIPERIDAE)UHRVATSKOJ:POPULACIJSKAEKOLOGIJA,
ODABIRMIKROSTANITAITERMOREGULACIJA
I.Vilaj,B.Lau,D.Jeli
HrvatskoherpetolokodrutvoHYLA,Zagreb,Hrvatska(vilaj22@gmail.com)
12.HBK19512 CBC
spolnojstrukturitetermoregulacijipremaspoluitipuobojenja.UOkujuriovkepoinjusvoju
aktivnostumjesecuoujku,auBegovomRazdoljuumjeseculipnju.
Kljunerijei:CaptureMarkRecapturemetoda,Viperaberus,ekolokepreferencije,spolna
struktura,meristikekarakteristike
I.Vilaj,B.Lau,D.Jeli
CroatianHerpetologicalSocietyHYLA,Zagreb,Hrvatska(vilaj22@gmail.com)
The Adder, Vipera berus (Linnaeus, 1785) is the most widespread and also the northernmost
distributedterrestrialsnakespeciesintheworld.InCroatia,therearetwosubspeciesoftheadder
in three separate populations. Field work was conducted on two different localities in Croatia:
Okuje (Turopolje, river Sava Valley; Vipera berus bosniensis) and Begovo Razdolje (Gorski kotar;
Viperaberusberus).ThemonitoringwasconductedbyCaptureMarkRecapturemethodduring
a four year period in Okuje (2009 2012), and a three year period in Begovo Razdolje (2009
2011). The main goals of this study were to determine if there are intrapopulation or
interpopulationsimilaritiesordifferencesinmicrohabitatpreferences,thermoregulation,dailyor
yearlyactivityperiod,genderstructureofpopulationandmeristiccharacteristics.Wecollected106
adders (50 from Begovo Razdolje and 56 from Okuje). The analyses have shown that there are
statisticallysignificantdifferencesbetweenthetwopopulationsinheadlength,headheightand
weight. The individuals from Okuje prefer a closed type of microhabitat, unlike individuals from
Begovo Razdolje that prefer an opened type of microhabitat. In both populations, there are
differences in the gender structure, and thermoregulation which is sex and colour morph
dependent.InOkuje,AddersbegintheiractivityperiodinMarchasopposedtoindividualsfrom
BegovoRazdoljewhoseactivityperiodbeginsinJune.
Key words: Capture Mark Recapture, activity period, gender structure, meristic
characteristics.
P59
GMAZOVIIVODOZEMCIVUGROVEKEOKOLICE
M.Zadravec
HrvatskoherpetolokodrutvoHYLA,Zagreb,Hrvatska(mladen.z123@gmail.com)
12.HBK19612 CBC
Kljunerijei:herpetofauna,Natura2000,"VejalnicaiKr"
REPTILESANDAMPHIBIANSOFTHEVUGROVECAREA
M.Zadravec
CroatianHerpetologicalSocietyHYLA,Zagreb,Croatia(mladen.z123@gmail.com)
FaunisticresearchofreptilesandamphibiansinCroatiaarenotsocommonplace,especiallyinthe
continental part. Here I present the results of an 11year inventarisation study of reptiles and
amphibians in the village of Vugrovec and its environs, near Zagreb, conducted from 2004 till
2014.ApartoftheresearchareafallsunderthenewNatura2000site"VejalnicaandKr"and
another one under the Significant landscape "Goranec". Both are being administered by the
Publicinstitution"Maksimir".Reptileswerecaughtbyhand,sometimeswiththeuseofspecial
gloves and snake hooks. Amphibians were caught by hand and handheld nets. The caught
individuals were photographed and released afterwards at the same spot in the shortest time
possible. A total of 11 reptile and seven amphibian species were recorded. One of the reptile
species is introduced the Hermann's tortoise, Testudo hermanni (Gmelin, 1789). It does not
occurnaturallyinthispartofCroatia.OfspecialimportanceisthefindingofBombinavariegata
(Linnaeus,1758),whichisonAnnexesIIandIVoftheEuropeanComission'sHabitatsDirective.
Theresultsofthisresearchcontributetotheknowledgeabouttheoccurenceanddistributionof
reptilesandamphibiansintheZagrebarea,andwithintheNatura2000site"VejalnicaandKr"
andtheSignificantlandscape"Goranec".TheycanalsobeofusetotheemployeesofthePublic
institution"Maksimir"forthecreationofmanagementplansforthetwosites.
Keywords:herpetofauna,Natura2000,"VejalnicaandKr"
P60
GMAZOVIIVODOZEMCIKAMENOLOMABIZEK
M.Zadravec1,2,R.Cesarec3,Z.Tadi1
1
Zavod za animalnu fiziologiju, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska
2
HrvatskoherpetolokodrutvoHYLA,Zagreb,Hrvatska(mladen.z123@gmail.com)
3
Treak6,Zagreb,Hrvatska
12.HBK19712 CBC
svojte.Odposebnogznaajajenalazutogmukaa,Bombinavariegata(Linnaeus,1758),kojije
na Dodacima II i IV Direktive o stanitima Europske komisije. Rezultati ovog istraivanja daju
doprinos poznavanju prisutnosti i rasprostranjenosti vrsta gmazova i vodozemaca na samome
rubugradaZagrebaiunutarParkaprirodeMedvednica.
Kljunerijei:herpetofauna,Natura2000,ParkprirodeMedvednica
REPTILESANDAMPHIBIANSOFTHEBIZEKQUARRY
M.Zadravec1,2,R.Cesarec3,Z.Tadi1
1
DepartmentofAnimalPhysiology,FacultyofScience,UniversityofZagreb,Zagreb,Croatia
2
CroatianHerpetologicalSocietyHYLA,Zagreb,Croatia(mladen.z123@gmail.com)
3
Treak6,Zagreb,Croatia
This was a study of reptiles and amphibians which lasted from 2008 till 2014 in the inactive
Bizek quarry, where we collected information on the presence of species belonging to those
twogroups.Avarietyofhabitatsoccurinthequarry:amixeddeciduousforestofQuercussp.,
Fagus sylvatica and Carpinus betulus surrounds the quarry to the north, east and west.
Thermophilicelementsarepredominantonthewesternside,whileonthesouthernsidethe
forestgiveswaytoyardsandhousing.Twopermanentstreamsandapondarealsopresent,
alongwithsomeintermittentstreamsandpuddles.Wecaughtanimalsbyhandand,insome
cases,wealsousedastickwithanooseforlizards,glovesandhooksforsnakes,andanetfor
the amphibians. We found seven reptile and seven amphibian species in the quarry, all of
which are already known from the surroundings of Zagreb. Frogs belonging to the genus
Pelophylax Fitzinger, 1843 were identified down to the genus level only, because of the
complexity of the determination and the inability to reliably identify them on the basis of
externalmorphology.ThefindingofBombinavariegata(Linnaeus,1758),whichisonAnnexes
IIandIVoftheEuropeanComission'sHabitatsDirective,isofspecialsignificance.Theresultsof
thisstudycontributetotheknowledgeoftheoccurrenceanddistributionofsuburbanreptiles
andamphibianswithintheNatureParkMedvednica.
Keywords:herpetofauna,Natura2000,NatureParkMedvednica
P61
NEWREVISIONOFTHECHECKLISTOFAMPHIBIANFAUNAINBOSNIAANDHERZEGOVINA
A.Zimi1,D.Jeli2,S.Lelo3,M.engi4
1
Herpetological Association in Bosnia and Herzegovina "Atra", Sarajevo, Bosnia and
Herzegovina(adnan.zimic@gmail.com)
2
CroatianHerpetologicalSocietyHYLA,Zagreb,Croatia
3
DepartmentofBiology,FacultyofScienceandMathematics,Sarajevo,BosniaandHerzegovina
4
Departmentofphysicalgeographyandecosystemscience,LundUniversity,Lund,Sweden
Research of batrahofauna of Bosnia and Herzegovina (B&H) has a long tradition. It can be
tracedbacktotheO.MellendorfPhDdissertationin1873.Thebatrachologicalinvestigations
inB&Hcouldbedivideonfourperiods:(1)TheperiodofOttomanEmpire/AustroHungarian
Monarchy(until1918),(2)TheperiodoftheKingdomofYugoslavia(19181943),(3)Theperiod
oftheSocialistFederalRepublicofYugoslavia(19431992)and(4)Theperiodofindependent
B&H. The main aim of this paper is to give an update of biosystematic information and to
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12.HBK19812 CBC
present the new precise distribution maps for all amphibian species in B&H. With new
information (literature and field data) we found significant differences comparative to the
previous reviews. Two new amphibian species were found, so there is evidence that 21
amphibianspecies(includingoneklepton)occursinB&H.Authorshaveconfirmedexistenceof
all21species.ThemostcommonspeciesarePelophylaxridibundusandBombinavariegataand
the rarest ones are Triturus carnifex, Triturus dobrogicus, Pelobates fuscus, Salamandra atra
and Triturus macedonicus, with just several findings. With 65% species of batrahofauna of
Balkan penninsula and two subendemic taxa (Proteus anguinus and Salamandra atra
prenjensis) B&H can be considered as important area of amphibian biodiversity in Southern
Europe.Newexpected(sub)speciesandsomebiosystematicproblemsarealsodiscussedinthis
paper.
Keywords:distribution,biosystematics,batrachology,BosniaandHerzegovina
BIOLOGIJAMORA
MARINEBIOLOGY
P62
UVIDIOEKOLOGIJIPREHRANESREDOZEMNEMEDVJEDICE
J.Antolovi1,E.Coppola2,L.Bundone2,S.alac1,D.kali1,V.StingaPerusco1,G.HernandezMilan3
1
Grupa sredozemna medvjedica, Udruga za istraivanje i zatitu prirode, Zagreb, Hrvatska
(jasna.antolovic@zg.tcom.hr)
2
GruppoFocaMonaca,Rim,Italija
3
UniversityCollegeCork,WesternRoad,Cork,Irska
Kljunerijei:sredozemnamedvjedica,uzorciizmeta,prehrana
INSIGHTSONTHEFORAGINGECOLOGYOFTHEMEDITERRANEANMONKSEAL
J.Antolovi1,E.Coppola2,L.Bundone2,S.alac1,D.kali1,V.StingaPerusco1,G.HernandezMilan3
1
The Mediterranean Monk Seal Group, Association for Wildlife Research and Protection,
Zagreb,Croatia(jasna.antolovic@zg.tcom.hr)
2
GruppoFocaMonaca,Roma,Italy
3
UniversityCollegeCork,WesternRoad,Cork,Ireland
TheMediterraneanmonksealisacriticallyendangeredmarinemammalspecieswhosehabitat
andbehaviorareingreatpartstillunknownintheMediterraneanandAdriatic.Inthisstudywe
present some insights about the foraging of this predator. Scat samples from north Adriatic
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12.HBK19912 CBC
(Croatia, n=24, 20102014) were collected and analyzed for dietary investigations. In all
samples,onlyfishbonesandscaleswererecovered.Mostofthepreysitems(36%)consiston
Sparidae fish were saddle sea bream and surmullet were the most common identified prey.
OtherremainswereonlypossibletoclassifyasPerciformsduetothedigestiondegreeofthe
bones. Although the study was carried out with a limited sample size, we underline the
importance of this information for an endanger mammal such as the Mediterranean Monk
Seal,andtheuseofscatsforthefirsttimeinthisspecies.
Keywords:TheMediterraneanmonkseal,Scatsamples,Theforaging
P63
Brooksia lacromae sp. nov. (Tunicata, Thaliacea), NOVA VRSTA DVOOTVORKE U
JADRANSKOMMORU
M.Batisti,R.Gari
Sveuilite u Dubrovniku, Institut za more i priobalje, Dubrovnik, Hrvatska
(mirna.batistic@unidu.hr)
Brooksia lacromae sp. nov. Gari & Batisti je opisana iz zooplanktonskog materijala
sakupljenog na stalnoj postaji u junom Jadranu u jesen 2014. Vrsta se morforloki znaajno
razlikuje od jedine dvije poznate vrste istog roda, B. rostrata i B. berneri. Genetika analiza
bazirananagenuza18SrRNApotvrdilajerazliitostvrsteB.lacromaeodvrsteB.rostrata(p
vrijednost izmeu sekvenci je bila 1,5%). U istim uzorcima pronaena je i atlantska vrsta
repnjaka Fritillaria helenae pa moemo pretpostaviti da su obje vrste
atlantskog/zapadnomediteranskog
podrijetla
te
su
mogui
indikatori
atlantsko/zapadnomediteranskestruje.
Kljunerijei:Thaliacea,novavrsta,Brooksialacromae,Jadranskomore
Brooksia lacromae sp. nov. (Tunicata, Thaliacea), A NEW SPECIES OF SALP FROM THE
ADRIATICSEA
M.Batisti,R.Gari
Institute for marine and coastal research, University of Dubrovnik, Croatia
(mirna.batistic@unidu.hr)
Brooksialacromaesp.nov.Gari&Batistiwasdescribedfromzooplanktonmaterialcollected
at a marine monitoring station in the South Adriatic in autumn 2014. This new species is
morphologicallymarkedlydifferentfromB.rostrataandB.berneri,sofartheonlytwospecies
in the genus Brooksia. Genetic analysis based on 18S rRNA gene confirmed distinctness (p
value between sequences 1.5%) of B. lacromae from B. rostrata. Occurrence of B. lacromae
withanAtlanticappendicularian,Fritillariahelenae,inthesamesamplessuggestsitsAtlantic
or Western Mediterranean origin and its potential for being an indicator species of
Atlantic/WesternMediterraneanwaters.
Keywords:Thaliacea,newspecies,Brooksialacromae,AdriaticSea
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12.HBK20012 CBC
P64
ZBIRKARAKOVAPRIRODOSLOVNOGMUZEJAUSPLITU
J.Boban,D.Vladovi
Prirodoslovnimuzejizoolokivrt,Split,Hrvatska(josip@prirodoslovni.hr)
Zbirku rakova u Prirodoslovnom muzeju u Splitu zapoeo je skupljati profesor Umberto
Giromettatenjezinpoetaknastajanjamoemosmjestitiu20tegodinedvadesetogstoljea.
Zbirka se temelji na uzorcima osam sakupljaa. Zbirka rakova Prirodoslovnog muzeja u Splitu
broji 409 primjeraka rasporeenih u 124 inventarna broja. Analizom je utvreno da zbirka
sadri 70 morskih vrsta rakova unutar 53 roda, 40 porodica i 6 redova. Vano je istaknuti 89
primjeraka koji su sakupljeni u Jadranu na znanstvenim ekspedicijama Instituta za
oceanografiju i ribarstvo: AdriaMed i MEDITS. Ostali primjerci su sakupljeni u osobnim
terenskimistraivanjimasakupljaa,advaprimjerkasudarovana.
Kljunerijei:Zbirkarakova,rakovi,Prirodoslovnimuzej,UmbertoGirometta
NATURALHISTORYMUSEUMCRUSTACEANCOLLECTIONINSPLIT
J.Boban,D.Vladovi
NaturalHistoryMuseumandZoo,Split,Croatia(josip@prirodoslovni.hr)
Crustacean collection of the Natural History Museum in Split began to collect Professor
UmbertoGiromettaanditsorigincanbedatedintheearly1920ies.Thecollectionisbasedon
samples from eight collectors. The collection of the Natural Museum in Split has 409 copies
distributed to 124 inventory numbers. The analysis shows that the collection contains 70
marine species of crustaceans within 53 genus, 40 families and 6 orders. It is important to
highlight89specimensthatwerecollectedintheAdriaticSeaonthescientificexpeditionsof
the Institute of Oceanography and Fisheries: AdriaMed and MEDITS. Other specimens were
collectedinthepersonalfieldresearchesofcollectors,andtwocopiesweredonated.
Keywords:Crustaceancollection,Crustaceans,NaturalHistoryMuseum,UmbertoGirometta
P65
MORFOLOGIJA I FILOGENIJA RODA Bacteriastrum (BACILLARIOPHYTA) U JADRANSKOM
MORU
S.Bosak1,L.upraha2,D.Nanjappa3,W.Kooistra3,D.Sarno3
1
Botaniki zavod, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska(suncica.bosak@biol.pmf.hr)
2
SveuiliteuUppsali,Uppsala,vedska
3
StazioneZoologicaAntonDohrn,Napulj,Italija
etirikolonijalnevrsteplanktonskihdijatomejarodaBacteriastrumB.furcatum,B.hyalinum,B.
jadranumiB.mediterraneumanaliziranesuuuzorcimaizJadranskogmoratesuiznjihuzgojene
kulture u laboratoriju. Opisana je morfologija i ultrastruktura frustula koristei svjetlosni i
elektronski mikroskop, te su dobivene sekvence velike podjedinice (LSU) rDNA. Opis vrste B.
jadranum je dopunjen i ispravljen na osnovi otkria novog i jedinstvenog naina formiranja
kolonijakodtevrstekojiukljuujepovezivanjestanicaulancepomouorgansketvari.Dopunjeni
suopisidvijeodtripreostalevrste;vegetativnestanicevrsteB.hyalinumposjedujukratkespinule
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12.HBK20112 CBC
u obliku slova Y na spojenim dijelovima seta i izdanke na valve u obliku slova T, dok mirujue
spore posjeduju nepravilnu povrinu vanjskog dijela omotaa primarne valve. Za vrstu B.
furcatum na eno je da posjeduje specifian raspored pora na povrini valve. Filogenetski
rezultati grupirali su LSU sekvence etiri vrste u dvije sestrinske grane; na prvoj se nalaze B.
furcatum(odjelSagittata)iB.hyalinum(odjelIsomorpha)doksunadrugojsmjeteniB.jadranum
(odjelIsomorpha)iB.mediterraneum(odjelSagittata).MoemozakljuitidajerodBacteriastrum
monofiletski rod, meutim odjeli ne dijele monofiletsko porijeklo te njihove pretpostavljene
odreujue taksonomske znaajke: izomorfne terminalne sete kod vrsta koje pripadaju odjelu
IsomorphatedimorfnekodvrstaodjelaSagitattanisusinapomorfije.
Kljunerijei:Planktonskedijatomeje,Bacteriastrum,Jadranskomore
S.Bosak1,L.upraha2,D.Nanjappa3,W.Kooistra3,D.Sarno3
1
DivisionofBotany,DepartmentofBiology,Zagreb,Croatia(suncica.bosak@biol.pmf.hr)
2
Paleobiology/DepartmentofEarthSciences,UppsalaUniversity,Uppsala,Sweden
3
StazioneZoologicaAntonDohrnNaples,Italy
Four colonial species of the multipolar centric diatom genus Bacteriastrum: B. furcatum, B.
hyalinum,B.jadranumandB.mediterraneumwereinvestigatedusingfieldsamplesfromthe
Adriatic Sea and cultured strains obtained from these samples. Morphology and frustule
ultrastructurewereexaminedusinglightandelectronmicroscopy,andnuclearencodedlarge
subunit(LSU)rDNAsequenceswereobtainedfromthestrains.ThedescriptionofB.jadranum
hasbeenemendedbasedonanovelmechanismofcolonyformationinwhichcellsarejoined
throughanorganiccelljacketwhichholdstogethercellsinchains.Descriptionsfortwoofthe
threeotherspeciesweresupplemented;vegetativecellsofB.hyalinumhaveshortYspinules
onthefusedpartofthesetaeandTshapedoutgrowths,andthesporesofthisspeciespossess
agranularsurfaceontheexteriorsideoftheprimaryvalvemantle.SpecificforB.furcatumis
the location of pores scattered around the setae bases. Phylogenetic results grouped the
sequences of the four species in a clade in which B. furcatum (section Sagittata) and B.
hyalinum (section Isomorpha) formed a clade as sister to a clade with B. jadranum (section
Isomorpha) and B. mediterraneum (section Sagittata). Apparently, neither section was
monophyletic,andthesupposeddefiningfeaturesofisomorphicterminalsetaeinIsomorpha
and dimorphic ones in Sagittata were not synapomorphies. Nonetheless, the genus
Bacteriastrumitselfremainedmonophyletic.
Keywords:planktonicdiatoms,Bacteriastrum,AdriaticSea
P66
SASTAV MAKROZOOBENTOSKIH BESKRALJENJAKA U OBRATAJNIM ZAJEDNICAMA U LUCI
SPLIT(HRVATSKA,JADRANSKOMORE)
M.Despalatovi,I.Cvitkovi,A.uljevi
Laboratorijzabentos,Institutzaoceanografijuiribarstvo,Split,Hrvatska(mare@izor.hr)
Istraivanje obratajnih zajednica u luci Split, vanoj hrvatskoj luci za meunarodni pomorski
promet, obavljeno je na dva teretna terminala (Sjeverna luka i Sveti Kajo) u proljee i jesen
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12.HBK20212 CBC
2014.godine.KoritenajemetodologijauzorkovanjakojasebaziranaCRIMPprotokolu,ana
pojedinom terminalu uzorci su sakupljani na tri transekta, meusobno udaljena 15 m, s
betonskih vertikalnih zidova, s dubina od 0,5 m, 3 m i 7 m, te iz sedimenta. Ukupno su
zabiljeene132vrstemakrozoobentoskihbeskraljenjaka,84naterminaluSjevernalukai108
na terminalu Sveti Kajo. Zabiljeene su dvije alohtone vrste: morski pu Siphonaria pectinata
(Linnaeus, 1758), prvi put zabiljeen u akvatoriju grada Splita 2003. godine; te kolonijalni
kamenikoraljOculinapatagonicadeAngelis,1908,prviputzabiljeenuluciSplit2011.godine.
Kljune rijei: obratajna zajednica, Siphonaria pectinata, Oculina patagonica, luka Split,
Jadranskomore
COMPOSITIONOFMACROZOOBENTHICINVERTEBRATESINFOULINGCOMMUNITIESINTHE
HARBOUROFSPLIT(CROATIA,ADRIATICSEA)
M.Despalatovi,I.Cvitkovi,A.uljevi
Laboratoryforbenthos,InstituteofOceanographyandFisheries,Split,Croatia(mare@izor.hr)
Key words: fouling community, Siphonaria pectinata, Oculina patagonica, harbour Split,
AdriaticSea
P67
TAKSONOMSKI SASTAV MORSKIH BENTOSKIH DIJATOMEJA I NJEGOV ODNOS S GLAVNIM
EKOLOKIMIMBENICIMAUBOSNIIHERCEGOVINI
N.Jasprica1,D.Hafner2,A.Car1,T.Kapetanovi3
1
Laboratorij za ekologiju planktona i populacijsku genetiku, Institut za more i priobalje,
SveuiliteuDubrovniku,Dubrovnik,Hrvatska(nenad.jasprica@unidu.hr)
2
Bartulovii4,Blace,Hrvatska
3
FondzazatituokoliaFederacijeBosneiHercegovine,Sarajevo,BosnaiHercegovina
Naistonojadranskojobalipostojisveganekolikostudijaomorskimbentoskimdijatomejama,
kojesuuglavnombileusmjerenenaistraivanjetaksonomijeiekologijeperifitonauhrvatskim
estuarijimaialbanskimslanimmovarama.Morskeplanktonskeibentoskedijatomejedosada
nisu nikada istraivane u Bosni i Hercegovini. Ova studija bila je dio veeg istraivakog
programa s ciljem utvrivanja bioraznolikosti te funkcioniranja ekosustava u pograninim
obalnim vodama izmeu Hrvatske i Bosne i Hercegovine. Program je ukljuivao algoloka,
zooloka i hidrografska istraivanja. U radu se prikazuju taksonomski sastav i sezonalnost
morskih bentoskih dijatomeja u odnosu na osnovne ekoloke parametre (temperatura,
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12.HBK20312 CBC
salinitet,gustoa)uNeumskomzaljevuuBosniiHercegovini.
Kljunerijei:Neumskizaljev,bentos,dijatomeje,istoniJadran,SISredozemlje
TAXONOMICCOMPOSITIONOFMARINEBENTHICDIATOMSINTHERELATIONTOSOMEKEY
ENVIRONMENTALVARIABLESINBOSNIAANDHERZEGOVINA
N.Jasprica1,D.Hafner2,A.Car1,T.Kapetanovi3
1
Laboratory of Plankton Ecology and Population Genetics, Institute for Marine and Coastal
Research,UniversityofDubrovnik,Dubrovnik,Croatia(nenad.jasprica@unidu.hr)
2
Bartulovii4,Blace,Croatia
3
Environmental Fund of the Federation of Bosnia and Herzegovina, Sarajevo, Bosnia and
Herzegovina
In the eastern Adriatic, there are only a few studies on benthic diatoms mostly focused on
ecology and taxonomy of periphytic diatoms in the Croatian estuaries or Albanian coastal
wetlands.Untilnow,bothplanktonicandbenthicmarinediatomshaveneverbeenstudiedin
Bosnia and Herzegovina. This study forms part of a research program investigating the
biodiversityandecosystemfunctioninginthebordermarinecoastalwatersbetweenthetwo
countriesCroatiaandBosniaandHerzegovina.Theprogramincludesphycological,zoological
andhydrographicalresearch.Thispaperconsidersthetaxonomiccompositionandseasonality
ofmarinebenthicdiatomsintherelationtosomekeyenvironmentalvariables(temperature,
salinity and density) in the Bay of Neum in Bosnia and Herzegovina on the southeastern
Adriaticcoast.
Keywords:NeumBay,benthos,diatoms,easternAdriatic,NEMediterranean
P68
DUBINSKA RASPROSTRANJENOST KORALJA U KORALIGENSKOJ BIOCENOZI ISTONOG
JADRANA
JedanodglavnihproblemavezanihuzspoznajeokoraligenskojzajedniciuJadranskommoruje
nedostatak podataka o dubinskoj rasprostranjenosti pojedinih vrsta. Koraligen se smatra
jednimodglavnih"vruihtoaka"biolokeraznolikostiuSredozemnommoru.Karakteriziragai
velik broj ugroenih dugoivuih vrsta (poput koralja), koje su vrlo osjetljive na antropogene
promjene, pogotovo zbog sporog rasta i neizvjesnog oporavka. Poetna dubina koraligenske
zajedniceovisiokoliinisvjetlostikojadopiredomorskogdna.Uzsmanjenukoliinusvjetlosti
(dovoljnezaveirazvojcrvenihalga(redCorallinales)),ponegdjepoetakkoraligenamoebiti
vrlo plitko. U Jadranskom moru, na podmorskim strmcima, najplia poetna dubina za
koraligensku biocenozu je oko 20 metara. Raznolikost vrsta koralja, vezano uz dubinsku
rasprostranjenost,ovisnajeonekolikoekolokihimbenika(morskestruje,koliinasvjetlosti,
temperatura i sedimentacija). Najee vrste koralja u ovoj biocenozi su Parazoanthus
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12.HBK20412 CBC
Kljunerijei:Koralji,koraligen,Jadranskomore
DEPTHDISTRIBUTIONOFCORALSINTHECORALLIGENOUSOFTHEEASTERNADRIATICSEA
Oneofthemajorgapsconcerningthecurrentstateofknowledgeofcoralligenouscommunity
in the Adriatic Sea is the scarcity of information on species bathymetrical distribution. The
coralligenousisconsideredasoneofthemostimportant"hotspots"ofspeciesdiversityinthe
Mediterranean.Itisalsocharacterizedbyharbouringagreatnumberofendangeredspecies,
which are longlived organisms, whose slow growth and recruitment rates make them very
sensitive to disturbances. The minimal depth for the formation of coralligenous concretions
dependsontheamountofirradiancereachingtheseabottom.Coralligenousconcretionscan
appearinveryshallowwatersiflightconditionsaredimenoughtoallowagreatdevelopment
ofcorallinealgae.IntheAdriaticSea,ontheverticalslopes,theminimaldepthreachesabout
20 meters. Differences among species of corals in tolerances to several physical factors
(currents, light, temperature and sedimentation) are thought to determine the upper and
lowerlimitsofspeciesdepthdistributions.ThemostcommoncoralspeciesareParazoanthus
axinellae, Epizoanthus arenaceus, Alcyonium coralloides, Alcyonium acaule, Paramuricea
clavata, Eunicella singularis, E. cavolini, Leptopsammia pruvoti, Hoplangia durotrix,
Caryophyllia inornata, C. smithii and Madracis pharensis. Populations of red coral (Corallium
rubrum)declinedinmostareasintheAdriaticSea,particularlyinshallowcoralligenous.
Keywords:Corals,Coralligenous,AdriaticSea
P69
TROFIKEINTERAKCIJEUZATIENIMMORSKIMPODRUJIMA:ULOGASTRANIHINVAZIVNIH
VRSTA
M.Mari1,M.DeTroch2,S.Olenin1
1
SveuiliteuKlaipedi,Klaipeda,Litva(martina.maric87@gmail.com)
2
SveuiliteuGentu,Biolokiodsjek,Ghent,Belgija
Cilj ovog istraivanja je procjena utjecaja invazivnih stranih vrsta (zelene alge Caulerpa
cylindracea, crvene alge Asparagopsis sp., desetononog raka Percnon gibbesi i pua
stranjokrnjakaAplysiadactylomela)uzatienommorskompodrujuPelagijskiotoci.Analizom
stabilnih izotopa bentikih organizama kvantificirane su trofike interakcije izmeu alohtonih i
autohtonih morskih vrsta. Usporedbom irina izotopskih nia i modela mijeanja izotopa
temeljenihnaBayesovojstatistici,istrailismopreklapaliseizotopskaniaherbivornihvrstaP.
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12.HBK20512 CBC
gibbesi i A. dactylomela s niama autohtonih herbivora i izraunali udio razliitih vrsta alga u
prehrani stranih i autohtonih vrsta. Rezultati su pokazali da je udio C. cylindracea izmeu 9% i
22%,anajveijeuprehraniP.gibbesiiA.dactylomela(16%i22%).Ovoistraivanjepokazaloje
dainvazivnestranealgepoveavajuraznolikostraspoloivogplijenaivjerojatnoolakavajuirenje
drugih stranih vrsta. Iako strane vrste nisu u izravnoj kompeticiji s autohtonim jeincima
Paracentrotus lividus i Arbacia lixula, mogua posljedica njihove velike brojnosti je smanjenje
energijedostupneorganizmimanaviemtrofikomnivouuhranidbenojmrei.
Kljune rijei: strane invazivne vrste, trofike interakcije, zatieno morsko podruje,
Sredozemnomore
TROPHICINTERACTIONSINMARINEPROTECEDAREAS:THEROLEOFINVASIVEALIENSPECIES
M.Mari1,M.DeTroch2,S.Olenin1
1
KlaipedaUniversity,Klaipeda,Lithuania(martina.maric87@gmail.com)
2
GhentUniversity,DepartmentofBiology,GhentUniversity,Ghent,Belgium
The objective of this study was to evaluate the effects of invasive alien species (green algae
Caulerpacylindracea,redalgaeAsparagopsissp.,herbivorouscrabPercnongibbesiandseahare
Aplysiadactylomela)inMPAPelagieIslands.Here,wepresenttheresultsofthestableisotope
analysis of benthic organisms in order to characterise trophic interactions between native and
alienspecies.UsingcomparisonsofspeciesisotopicnichewidthsandBayesianmixingmodels,
weinvestigatedwhetherthedietofalienherbivoresoverlappedwiththoseofnativespeciesand
quantified the food source contribution to diets of native and alien consumers. Mixing models
showed that contribution of C. cylindracea is between 9% and 22%. Highest contributions are
thoseindietsofP.gibbesiandA.dactylomela(16%and22%,respectively).Resultsindicatedthat
invasion of macroalgae is increasing the diversity of available prey and facilitates spread of
another alien species. Although P. gibbesi is not in direct competition with native sea urchins
Paracentrotus lividus and Arbacia lixula, high abundances of this invasive crab are likely to be
reducingtheenergyavailabletohigherconsumersinthefoodweb.
Key words: invasive alien species, herbivores, trophic interactions, marine protected area,
Mediterranean
P70
DNEVNI PRIRASTI OTOLITA KAO METODA VALIDACIJE TONOG ODREIVANJA STAROSTI
TRLJEODKAMENA,Mullussurmuletus
S.MatiSkoko1,J.Ferri2,N.Staglii1,P.Tutman1,D.BrajiJurica3
1
Institutzaoceanografijuiribarstvo,Split,Hrvatska(sanja@izor.hr)
2
SveuiliteuSplitu,Odjelzastudijemora,Split,Hrvatska
3
RibarskazadrugaFrikariba,Split,Hrvatska
Postojipojaanapotrebazarelevantnom,vjerodostojnommetodomodreivanjastarostikod
trlje od kamena, Mullus surmuletus kao ciljane vrste mediteranskog priobalnog ribarstva.
Ukupno je analizirano 387 jedinki ukupnih duina od 6,8 do 32,9 cm. Godinja periodinost
nastanka prstenova potvrena je analizom rubnog prirasta. Godinji rast otolita je verificiran
analizomtiparuba.Udiootolita s tamnimrubomjebionajveizimi(>80%). Mjesenisrednji
rubniprirastjebionajmanjiulipnju.Analizamjerenihudaljenostijepokazaladaseprvipravi
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12.HBK20612 CBC
prstenpojavljujenaudaljenostiod0,78mm(0,104SD)odcentraipristarostiribeod0,83
godina. Analiza starosti je utvrdila 6 starosnih razreda i tonost je potvrena brojanjem
dnevnih prirasta. Svi su mjereni parametri otolita bili linearni sa starosti. Najpreciznija je
procjenazaanaliziranupopulacijudobivenaizirineotolita,aslijedilajeduinaimasa.Rastje
procijenjenVonBertalanffyomjednadbomrasta.Rastjebionajbridodrugegodineivota,a
onda usporava. Uzimajui u obzir utvrene bioloke odrednice, trenutno gospodarenje je
potrebno nadopuniti cjelovitijim, ekolokim pristupom propisima, kako bi se osigurale
uinkovitemjerezatitezaovuekonomskiiekolokivanuvrsturiba.
Kljunerijei:dnevniprirast,starost,otolit,Mullussurmuletus
S.MatiSkoko1,J.Ferri2,N.Staglii1,P.Tutman1,D.BrajiJurica3
1
InstituteofOceanographyandFisheriesSplit,Croatia(sanja@izor.hr)
2
UniversityofSplit,CenterofMarineStudies,Split,Croatia
3
FishermenCooperativeFreshFish,Split,Split,Croatia
There is a high need to establish a method for reliable age determination of the striped red
mullet,MullussurmuletusastargetfishspeciesofMediterraneanartisanalfisheries.Intotal,387
specimensrangingintotallengthfrom6.8to32.9cmwereanalyzed.Theannualperiodicityof
annulus deposition was supported by the marginal increment analysis. The annual growth of
otoliths was verified using edgetype analysis. The proportion of otoliths with opaque margins
was the highest (>80%) in the winter. The monthly mean marginal increment showed a single
minimum inJune.Adistancemeasurements analysis revealed that first regularringappearsat
distanceof0.78mm(0.104SD)fromotolithnucleusandfishageofabout0.83years.Theage
analysis revealed 6 age classes. Age determination was validated by daily otoliths increments
readings. All measured otolith morphometric parameters were linear with fish age. The most
preciseageestimationsofanalyzedpopulationwereobtainedfromtheotolithwidth,followedby
theotolithlengthandmass.ThegrowthwasestimatedbythefitoftheVonBertalanffygrowth
function. The growth of M. surmuletus was rapid up to 2 years of age and then slowed.
Consideringtheidentifiedbiologicalimplications,existingmanagementshouldbecomplemented
byamoreholistic,ecosystemapproachtoregulations,inordertoassureeffectiveconservation
measuresforthiseconomicallyandecologicallyimportantfishspecies.
Keywords:dailyincrements,age,otolith,Mullussurmuletus
P71
PROCJENABROJNOSTIZAJEDNICEDOBRIHDUPINA(Tursiopstruncatus)VIKOGAKVATORIJA
J.MioiStoi1,G.Plesli2,N.RakoGospi2,C.M.Fortuna3,D.Holcer4
1
PlavisvijetVis,Vis,Hrvatska(jure.miocicstosic@plavisvijetvis.hr)
2
PlavisvijetInstitutzaistraivanjeizatitumora,VeliLoinj,Hrvatska
3
Institutzazatituiistraivanjeokolia,Rim,Italija
4
Hrvatskiprirodoslovnimuzej,Zagreb,Hrvatska
12.HBK20712 CBC
odproljeadojeseniplovilomduljine6m.Opaenoje327skupinadobrihdupina,ametodom
fotoidentifikacije je utvrena prisutnost 723 jedinke. Brojnost populacije procijenjena je
metodom ulova i ponovnog ulova za zatvorene populacije pri emu je koriten Chao Mth
procjenitelj. U analizu su uvrtene samo jedinke s izraenim prirodnim oznakama kako bi se
umanjilavjerojatnostpogreneidentifikacije.Rezultatiukazujunatodajebrojnostpopulacije
uovomrazdobljubilastabilnabuduidausporedbomutvrenihvrijednosti nijeustanovljena
statistiki znaajna razlika izmeu pojedinih godina (Ztest; z<1,829, P>0,0674 za sve
usporedbe).Natemeljupodatakaiz2014.godine,procijenjenabrojnostpopulacijeiznosi278
(95% CI=208413) jedinki dobrih dupina. Budui da je dio istraivanog podruja uvrten u
Natura 2000 mreu s ciljem ouvanja dobrih dupina, potrebno je osigurati nastavak praenja
brojnostipopulacijeuovompodruju.
Kljunerijei:dobridupin,procjenabrojnosti,zatienopodruje
ABUNDANCEESTIMATESOFTHEBOTTLENOSEDOLPHIN(Tursiopstruncatus)COMMUNITYIN
VISARCHIPELAGO
J.MioiStoi1,G.Plesli2,N.RakoGospi2,C.M.Fortuna3,D.Holcer4
1
BlueWorldVis,Vis,Croatia(jure.miocicstosic@plavisvijetvis.hr)
2
BlueWorldInstituteofMarineResearchandConservation,VeliLoinj,Croatia
3
InstituteforEnvironmentalProtectionandResearch,Rome,Italy
4
CroatianNaturalHistoryMuseum,Zagreb,Croatia
Westudiedthebiologyandecologyoftheresidentpopulationofbottlenosedolphins(Tursiops
truncatus) in the area around Vis Island (~5,500 km2) between 2007 and 2014. Data was
collected in the spring and summer using a 6 meter rigid inflatable boat. We obtained 327
sightings and photoidentified 723 individuals. Abundance of the bottlenose dolphin
population was estimated using the markrecapture model for closed populations and Mth
estimator of Chao. To avoid misidentification errors, only wellmarked individuals were
includedintheanalysis.Nosignificantdifferenceintheyeartoyearabundancewasdetected
(Ztest;z<1.829,P>0.0674forallcomparisons),sothepopulationappearedstableduringthe
studyperiod.Basedondatacollectedin2014,thepopulationabundanceestimateis278(95%
CI=208413) bottlenose dolphins. As a part of the research area was included in the Natura
2000 network with the aim of conserving the local bottlenose dolphin population, it is
necessarytocontinuewithmonitoringofthepopulationabundanceinthisarea.
Keywords:bottlenosedolphin,abundanceestimate,protectedarea
P72
STANJE POPULACIJE INUNA (Engraulis encrasicolus L. 1758) U ISTONOM DIJELU
JADRANSKOGMORA
B.Musta
Sveuilite u Zadru, Odjel za ekologiju, agronomiju i akvakulturu, Zadar, Hrvatska
(bmustac@unizd.hr)
12.HBK20812 CBC
ulovinunauRHje2011.godinebio14163t,dokje2013.godineiznosio8904tone.Svrhaje
ovogradautvrditistanjepopulacijeinunauistonomdijeluJadrana.Analiziranojeukupno612
jedinkiuzorkovanihodlipnjadoprosinca2014.godine.Sviuzorcisupotjecaliizlovinaplivarica,
ostvarenihuribolovnimzonamaB,C,EiFistonog,hrvatskogdijelaJadranskogamora.Totalne
duine (LT) svih jedinki su se kretale u rasponu od 10,0 do 16,6 cm. Srednja vrijednost je
iznosila 13,5 0,99 cm, a modalni duinski razred 13,0 cm. Odnos spolova je bio u korist
mujaka; iznosio je m/=1,63. Analizom duinskomasenog odnosa utvren je pozitivan
alometrijski rast (b=3,469; r2=0,899). Kubini koeficijent kondicije (K) se kretao u rasponu od
0,52 do 0,69, te je pokazao, ukupno gledajui, poveanje vrijednosti s porastom duine ribe.
Poetkomovogistraivanja,ulipnju,inunsemrijestio,afazaspolnogmirovanjajezapoela
nakonrujna.
Kljunerijei:inun,Jadranskomore,duinskomasenidonos,kondicija
B.Musta
University of Zadar, Department of ecology, agronomy and aquaculture, Zadar, Croatia
(bmustac@unizd.hr)
Anchovy Engraulis encrasicolus L. 1758, is one of the most abundant species in Croatian
fisheries. However, in the last few years, landings of this ecologically and commercially very
importantspecieshavedecreasedfrom14163tonsin2011to8904tonsin2013.Theaimof
this study was to analyse current state of anchovy population in the Eastern Adriatic Sea. In
total, 612 anchovy specimens were sampled from June to December 2014. All fishes were
sampledfrompurseseinelandings,i.e.fromfisherieszonesB,C,EandFoftheEasternAdriatic
Sea.Totallength(LT)variedfrom10.0to16.6cm,theaveragelengthwas13.50.99cm,and
modal length class 13.0 cm. Number of male specimens exceeded the number of female
specimens(m/f=1.63).Analysesoflengthweightrelationindicatedpositiveallometricgrowth
(b=3.469; r2=0.899). Condition coefficient (K) ranged from 0.52 to 0.69. In general, condition
values were higher in longer fishes. In the beginning of this study, in June, anchovy was
spawning,andinactivephaseofmaturitycyclestartedafterSeptember.
Keywords:Anchovy,AdriaticSea,Lengthweightrelation,Conditioncoefficient
P73
PRAENJE RADIOEKOLOKOG STANJA SJEVERNOG JADRANA KORITENJEM DAGNJE Mytilus
galloprovincialisKAOINDIKATORA
D.PaviiHamer
CIM,IRB,Rovinj,Hrvatska(pavicic@cim.irb.hr)
12.HBK20912 CBC
lokacijama pod izrazitim utjecajem dotoka slatkih voda: Limski kanal, Raki i Bakarski zaljev. U
morskoj vodi Limskog kanala koncentracije aktivnosti 137Cs su bile niske (1,83Bqm3). Na svim
lokacijamakoncentracijeaktivnosti 137Csudagnjikretalesuseispodgranicadetekcije(<0,3Bq
kg1). Koncentracije aktivnosti 7Be u dagnji su u proljee vie od aktivnosti mjerenih u jesen.
Koncentracijeaktivnosti 40Kudagnjisunetovieujesensaprosjenomaktivnosti313Bqkg1.
Koncentracijeaktivnosti 232Th(228Ra), 238Ui 226Raudagnjiizrazitosuniskeibitnosesezonskine
razlikuju.Povienekoncentracijeaktivnostipojedinihradionuklidautkivudagnjidokazujunjenu
iznimnu sposobnost biokoncentracije/biomagnifikacije zagaivala i potencijalno koritenje kao
indikatora.RadioekolokostanjesjevernogJadranajezadovoljavajueinemaznaajnijegunosa
radionuklidaatmosferskimzagaivanjemirijekama.
Kljunerijei:sjeverniJadran,Mytilusgalloprovincialis,radionuklidi
D.PaviiHamer
CMR,IRB,Rovinj,Croatia(pavicic@cim.irb.hr)
The source of radionuclides in the Adriatic Sea is global fallout primarily from the Chernobyl
accident in 1986 and indirectly from the rivers discharges. Mussel Mytilus galloprovincialis is
used as an indicator organism in monitoring marine pollution programs. Systematic
radioactivity monitoring in marine indicator organisms was conducted by determination
activity concentrations of artificial 137Cs, natural 40K, 232Th (228Ra), 226Ra, 238U and cosmogenic
7
Beinthetissuesofmussels.Themusselswerecollectedinspringandautumnperiodsfrom
2008to2014atlocationsundersignificantfreshwaterdischarges:Lim,RaaandBakarbay.In
Lim Bay 137Cs activity concentrations in surface seawater were low (1.83Bqm3). In mussels
137
Csactivityconcentrations(<0.3Bqkg1)werebelowthedetectionlimitinalllocations.Inthe
spring activity concentrations of 7Be in mussels were greater than in autumn. In the autumn
activityconcentrationsof40Kinmusselswerehigherthaninthespring,theaverage40Kactivity
was313Bqkg1.Activityconcentrationsof 232Th(228Ra), 238Uand 226Rainmusselsweremostly
below the detection limit with no seasonal changes. Increased activity concentrations of
individual radionuclides in the mussel tissues proved their exceptional ability of pollutant
concentrationandpotentialindicatorusage.RadioecologicalconditionofthenorthernAdriatic
Seaissatisfactorywithoutsignificantradionuclidedischargesfromfalloutandrivers.
Keywords:ThenorthernAdriaticSea,Mytilusgalloprovincialis,radionuclid
P74
POJAVNOSTIGUSTOADOBRIHDUPINA(Tursiopstruncatus)UMURTERSKOMMORU
G.Plesli1,A.Impetuoso2,C.Fortuna1
1
Plavi svijet Institut za istraivanje i zatitu mora, Veli Loinj, Hrvatska (grgur.pleslic@plavi
svijet.org)
2
MarineLifeConservation,Imperia,Italija
Uvremenuod2002.do2007.(P1)iod2013.do2014.(P2)istrailismopojavnost,stopeopaanja
i relativnu gustou dobrih dupina (Tursiops truncatus) u Murterskom moru. Vizualne pretrage
terenaprovedenesusjedriliceprosjenebrzinekretanjaod3,8vuperioduP1,tegumenjakom
prosjenebrzinekretanjaod12,5vuperioduP2.NavigacijskipodatcibiljeenisuGPSureajem,
th
12.HBK21012 CBC
aopaanjadupinakameromsfilmomod2002do2004,tedigitalnomkameromod2005.nadalje.
Prijeeniputtijekompretraivanjaiznosioje1931kmuP1,te2186kmuP2.Uvremenu2002.
2007. zabiljeeno je 37 opaanja dobrih dupina, te 50 opaanja tijekom 2013.2104. Prosjena
veliina skupine iznosila je 6,2 jedinke u P1 i 5,5 jedinki u P2. Primjenom metode foto
identifikacije,naosnovi84jedinkeopaeneuP1i127jedinkiutvrenihuP2izraenjekatalogod
194 dobra dupina. Ukupno je 17 jedinki opaano u oba perioda (8,8%). Krivulja otkrivanja
temeljenanasvimopaanjimanepokazujeasimptotskirast.Prosjenastopaopaanja,izraena
kaobrojopaanjapo1kmprijeenogputatijekompretrage,iznosilaje0,0397uP1i0,023uP2.
Relativna gustoa, izraena kao broj identificiranih jedinki po 1 km prijeenog puta tijekom
pretrage,iznosilaje0,0947uP1i0,0797uP2.RezultatipokazujudajeMurterskomoreznaajano
podruje za dobre dupine. S obzirom na intenzitet nautikog turizma u ovom podruju, ovo
istraivanjepruasmjernicezadaljnjimonitoringizatitu.
Kljunerijei:dobridupin,Tursiopstruncatus,Jadranskomore,fotoidentifikacija,zatita
OCCURRENCEANDDENSITYOFBOTTLENOSEDOLPHINS(Tursiopstruncatus)INMURTERSEA
G.Plesli1,A.Impetuoso2,C.Fortuna1
1
Blue World Institute of Marine Research and Conservation, Veli Loinj, Croatia
(grgur.pleslic@plavisvijet.org)
2
MarineLifeConservation,Imperia,Italy
Westudiedbottlenosedolphins(Tursiopstruncatus)inthewaterssurroundingtheIslandof
Murter (Murter Sea, Croatia) in two periods: 20022007 (P1), and 20132014 (P2), and
obtained baseline data on occurrence, encounter rates and relative densities. Visual area
surveysinsearchfordolphinswereconductedusingasailingboatwiththeaveragesearch
speedof3.8ktsintheP1,and5.2mrigidhullinflatableboatwiththeaveragesearchspeed
of 12.5 kts in the P2. Navigation data were collected using GPS, while observations of
dolphins were documented using film camera from 2002 to 2004 and digital camera from
2005 onwards. Research effort resulted in 1931 km in search and 37 sightings in P1, and
2186kminsearchand50sightingsinP2.Averagegroupsizewas6.2individualsinP1,and
5.5 in P2. Photoidentification analysis resulted in a catalogue of 194 individuals. In P1, 84
individualswereidentified,127individualsinP1,while17animalswereseeninbothperiods
(8.8%). Discovery curve based on all sightings shows no asymptotic growth. Relative
encounter rate, expressed as number of sightings per km on effort, was 0.0397 in P1 and
0.023 in P2. Relative linear density, expressed as number of identified dolphins per km on
effortwas0.0947inP1and0.0797inP2.TheresultsshowthattheMurterSeaisimportant
forlocalbottlenosedolphins.Giventheintenselevelsofnauticaltourisminthisarea,these
resultscanserveasguidelinesforsubsequentmonitoringanddevelopmentofconservation
plans.
Keywords:Bottlenosedolphin,Tursiopstruncatus,AdriaticSea,photoidentification,conservation
P75
RASPROSTRANJENOST, ABUNDANCIJA I RAZMNOAVANJE VRSTE Siphonaria pectinata
(GASTROPODA,PULMONATA)UAKVATORIJUGRADASPLITA
I.Radoni,I.Cvitkovi,M.Despalatovi
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12.HBK21112 CBC
Institutzaoceanografijuiribarstvo,Split,Hrvatska(ivana.radonic.88@gmail.com)
Kljunerijei:Siphonariapectinata,rasprostranjenost,abundancija,razmnoavanje,Jadranskomore
I.Radoni,I.Cvitkovi,M.Despalatovi
Institutzaoceanografijuiribarstvo,Split,Hrvatska(ivana.radonic.88@gmail.com)
Marine snail Siphonaria pectinata (Linnaeus, 1758) (Gastropoda, Pulmonata) inhabits rocky
intertidalhabitatsinwarmerareasonbothsidesoftheAtlantic,andtheAlboranSea.Inthe
centralAdriatic,inthewatersoftheSplitarea,thisinvasivespecieswasrecordedforthefirst
timeinthespringof2003,probablyintroducedbyships.Sinceitspopulationwasnumerous,it
was assumed that it was settled in this area much earlier. In 2009 was recorded in the area
from Rogoznica to Omi and the island of Bra. A research of distribution, abundance,
biometric features and reproduction period of this species had been conducted at the three
stations in the city of Split (peninsula Marjan). In the investigated area this species inhabits
rockybottomofmediolittoralanditismorenumerousintheuppermediolittoral.Themean
abundanceofthespecieswas64organisms/400cm2(n=720).Themeanlengthofindividual
organismswas1.10.4cm(n=4883).TherecordedreproductionperiodofS.pectinatainthe
studyareawasfromMaytoOctober,whenitlaidcocoons.
Keywords:Siphonariapectinata,distribution,abundance,reproduction,AdriaticSea
P76
UTJECAJ STRATEGIJE HRANJENJA NA DRUTVENU STRUKTURU DOBRIH DUPINA (Tursiops
truncatus)UISTONOMJADRANU,HRVATSKA
G.Plesli1,G.Kett2,N.RakoGospi1,T.Vuur1,D.Holcer1,J.MioiStoi1,P.Mackelworth1
1
Plavi svijet Institut za istraivanje i zatitu mora, Veli Loinj, Hrvatska (grgur.pleslic@plavi
svijet.org)
2
UniversityCollegeofCork,Cork,Irska
Ranijestudijeopisujuutjecajkoarenjailimarikulturenaveliinuisastavskupinadobrogdupina
(Tursiops truncatus), no do sada nije promatran kombinirani utjecaj. Tijekom 2013. i 2014.
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12.HBK21212 CBC
Kljunerijei:dobridupin,Tursiopstruncatus,socijalnaekologija,ribarstvo,zatita
INFLUENCEOFFORAGINGSTRATEGIESONSOCIALSTRUCTUREOFBOTTLENOSEDOLPHINS
(Tursiopstruncatus)INEASTERNADRIATICSEA,CROATIA
G.Plesli1,G.Kett2,N.RakoGospi1,T.Vuur1,D.Holcer1,J.MioiStoi1,P.Mackelworth1
1
Blue World Institute of Marine Research and Conservation, Veli Loinj, Croatia
(grgur.pleslic@plavisvijet.org)
2
UniversityCollegeofCork,Cork,Ireland
Previous studies have shown that either bottom trawlers or fish farms influence bottlenose
dolphin (Tursiops truncatus) group size and structure, however no study described combined
effect.In2013and2014aphotoIDbasedstudywasconductedinthreeregionsoftheAdriatic
sea: CresLoinj, North Dalmatia and Vis. The aim was to examine combined effect of bottom
trawlers and fish farms upon group size, social and ageclass structure of bottlenose dolphin
communities in the three regions that differ in intensity of bottom trawler and aquaculture
fisheries. Research effort resulted in sightings of 176 groups engaged in "open water foraging"
(OWF),59in"bottomtrawlerforaging"(BTF)and8in"foragingatfishfarm"(FFF).Overallgroup
sizewas50.56,beinghighestinCresLoinj(5.810.43)andlowestinVisregion(3.60.73),butit
did not vary significantly between the foraging strategies (K=0.138, df=2, p>0.05). Simpleratio
associationindexwasoveralllow(0.10.1),buthigherforFFF(0.070.03)andBTF(0.040.02)
than OWF groups (0.020.01). Hierarchial cluster analyses showed overall highly structured
population (CCC=0.91), structure being more affected by presence of bottom trawlers and fish
farms in North Dalmatia and Vis than in CresLoinj region. Age composition of groups varied,
with moreadults inBTFandFFF thaninOWFgroups (2=9.21,p<0.001). These results provide
baselineinformationforfurtherresearchandconservationplans.
Keywords:bottlenosedolphin,Tursiopstruncatus,socialecology,fisheries,conservation
P77
POVEZANOSTPODVODNEBUKEUCRESKOLOINJSKOMAKVATORIJUSAPROMJENAMAU
GLASANJUDOBRIHDUPINA(Tursiopstruncatus)
N.RakoGospi,G.Plesli,S.Stipi,J.Corbin,M.Picciulin
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12.HBK21312 CBC
Plavi svijet Institut za istraivanje i zatitu mora, Veli Loinj, Hrvatska (nikolina.rako@plavi
svijet.org)
Kljunerijei:Dobridupini,podvodnabuka,zviduk,frekvencija,plovila
SHIFTSINBOTTLENOSEDOLPHIN(Tursiopstruncatus)VOCALIZATIONINRELATIONTOSEA
AMBIENTNOISEINTHECRESANDLOINJWATERS,CROATIA
N.RakoGospi,G.Plesli,S.Stipi,J.Corbin,M.Picciulin
Blue World Institute of Marine Research and Conservation, Veli Loinj, Croatia
(nikolina.rako@blueworld.org)
IntheCresLoinjarchipelago(northeastAdriaticSea,Croatia)passiveacousticmonitoringwas
appliedtocharacterizelocalseaambientnoise(SAN)andassesstherelationshipbetweenthe
underwaternoiseandtheacousticbehaviour(whistles)ofresidentbottlenosedolphingroups.
Between2007and2009,systematicmonthlysamplingofSANwasmadeattenlocationsusing
RESONTC4032hydrophoneconnectedtoapreviouslycalibratedPioneerDC88DATrecorder.
Boatpresence,typeanddistancefromthemonitoringstationwerenotedalongwhilstacoustic
monitoring was ongoing. Acoustic recordings containing dolphin whistles were analyzed. The
followingwhistleparameterswereconsidered:duration,maximumandminimumfrequencies,
delta frequencies, start and end frequencies, number of inflection points and the number of
harmonics.Whistleparameterswerecomparedtothecharacteristicsofseaambientnoiseof
the acoustic samples containing them. Results indicated that dolphin produce whistles at
higherfrequencieswhenambientnoiselevelsatlowfrequencieswerehigher.Variationsofthe
whistle structure resulted to be related to the overall presence of boats. When boats were
present, dolphinsproducedwhistleswithlongerdurationatsignificantlyhigherfrequencies.
These changes in whistle structure were found to be dependent on the group behavioural
state,inparticularduringtheirforagingandsocializingactivities.
Keywords:Bottlenosedolphins,seaambientnoise,whistle,frequency,boats
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12.HBK21412 CBC
P78
MALAKOLOKAZBIRKAPETRANOVAKA
S.Vrgo,N.evrnja
Prirodoslovnimuzejizoolokivrt,Split,Hrvatska(sanja@prirodoslovni.hr)
PrirodoslovnimuzejizoolokivrtuSplituposjeduje7malakolokihzbirkiodkojihjejednau
stalnompostavu(MalakolokazbirkaobiteljiBakoti)te6deponiranih:zbirkeP.Novaka,A.
Cvitania,M.Kolander,J.Save,V.GolubiaidonB.Cvitanovia.PetarNovak(1879.1968.),
hrvatskiagronomistrunjakzazatitubilja,biojeravnateljPrirodoslovnogmuzejauSplitu
(1946.1952.). Cijenjen entomolog, specijalist za kornjae (Coleoptera), otkrio je vei broj
endemskih vrsta kukaca, a oko 30 vrsta dobilo je po njemu ime. Vie od 40 strunih i
znanstvenihradovaobjaviojeuTrstu,Beu,Rimu,Zagrebu,SarajevuiBeogradu.Meutim,
malo je poznato da je prikupio i malakoloku zbirku. Zbirka je nastala neposredno nakon
Drugogsvjetskograta(1946.1949.)iinejepredstavnicipuevaikoljkaaJadranskogmora
i kopnenih pueva. Primjerci su sakupljeni na podruju Splita i srednjodalmatinskih otoka
(Suac, Svetac, Palagrua, olta) i to poglavito u plitkom litoralnom podruju. Zbirka se
sastojiod364primjerkarasporeenihu45inventarnihbrojeva,svrstanihu23porodicei32
roda.NajzastupljenijeporodicesuVeneridae,Donacidae,Tellinidae,Helicellidae,Nassariidae
iHygromiidae.Svisuprimjercideterminirani,inventariziraniiuneseniubazupodataka.
Kljunerijei:PetarNovak,malakolokazbirka,Prirodoslovnimuzej
MALACOLOGICALCOLLECTIONOFPETARNOVAK
S.Vrgo,N.evrnja
TheNaturalHistoryMuseumandZOO,Split,Croataia(sanja@prirodoslovni.hr)
TheNaturalHistoryMuseumandZOOinSplitholds7malacologicalcollectionsofwhichone
is permanently exhibited and the other ones are deposited: collections of P. Novak, A.
Cvitani, M. Kolander, J. Savo, V. Golubi and B. Cvitanovi. Petar Novak (18791968),
Croatian agronomist and an expert for plant protection, was the principle of The Natural
History Museum in Split (19461952). This admired entomologist (coleopterologist)
discovered many endemic species and around 30 species were named after him. He
published more than 40 scientific papers in Trst, Vienna, Rome, Zagreb, Sarajevo and
Belgrade.However,it'snotknownthathealsohadamalacologicalcollection.Thecollection
was created immediately after World War II (19461949) and contains molluscs and
gastropods of The Adriatic Sea and land snails. The specimens were collected in the Split
area, on middle dalmatian islands (Suac, Svetac, Palagrua, olta) mostly in the shallow
littoral area. Collection consists of 364 specimens organised in 45 inventory number,
presenting23familyand32genus.ThemostrepresentedfamilywereVeneridae,Donacidae,
Tellinidae, Helicellidae, Nassariidae and Hygromiidae. All the specimens are determined,
inventoriedandstoredinadatabase.
Keywords:PetarNovak,malacologicalcollection,NaturalHistoryMuseum
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6.SIMPOZIJHRVATSKOGDRUTVAZABILJNUBIOLOGIJU
6THSYMPOSIUMOFCROATIANSOCIETYOFPLANTBIOLOGISTS
P79
FOTOSINTETSKA UINKOVITOST, UDIO FOTOSINTETSKIH PIGMENATA I FENOLNIH SPOJEVA
TE ANTIOKSIDACIJSKA AKTIVNOST U ROSIKA (Droseraceae) PRI RAZLIITIM UVJETIMA
UZGOJA
M.Curman,M.Tuek,M.Babi,M.Tkalec
Sveuilite u Zagrebu, Prirodoslovnomatematiki fakultet, Bioloki odsjek, Zagreb, Hrvatska
(marcela.curman@gmail.com)
Kako bi nadoknadile manjak nutrijenata na stanitu, rosike (Droseraceae) mame i love kukce
ljepljivimkapljicamanatentakulimalistova.Uradujeistraenuinakrazliitihuvjetauzgojana
fotosintetsku uinkovitost, koliinu fotosintetskih pigmenata i fenolnih spojeva, te
antioksidacijsku aktivnost plojki i peteljki listova u vrstama Drosera spatulata i D. aliciae. Dio
biljaka bio je uzgajan u sobnim uvjetima pri niskim intenzitetima osvjetljenja uz smanjenu
mogunosthvatanjakukacadokjedrugidiobiljakauzgajannaotvorenomprostoruprivisokim
intenzitetimaosvjetljenjauzveudostupnostkukaca.Plojkelistovaimalesuviufotosintetsku
uinkovitostteviisadrajfotosintetskihpigmenataifenolnihspojevaodpeteljkianaliziranih
vrsta. Iako je sadraj klorofila i karotenoida bio vii u biljaka uzgajanih u sobnim uvjetima,
fotosintetska uinkovitost bila je vea u biljaka koje su rasle na otvorenom, vjerojatno zbog
izloenosti viim intenzitetima svjetlosti i/ili vee dostupnosti kukaca. Udio flavonoida i
antocijanabiojeveiuplojkamabiljakauzgajanihnaotvorenom,tojerezultiralopoveanjem
sadrajaukupnihfenolauvrsteD.spatulatadokjeuvrsteD.aliciaeviisadrajukupnihfenola
naenubiljakauzgajanihusobnimuvjetima.Antioksidacijskaaktivnostuekstraktimaplojkii
peteljki bila je visoka u biljkama koje su rasle u sobnim uvjetima kao i kod biljaka na
otvorenom,osimuekstraktimapeteljkivrsteD.spatulatauzgajaneusobnimuvjetima.
Kljunerijei:Droseraceae,fotosinteza,klorofili,fenolnispojevi
M.Curman,M.Tuek,M.Babi,M.Tkalec
University of Zagreb, Faculty of Science, Department of Biology, Zagreb, Croatia
(marcela.curman@gmail.com)
Droseraceae(sundews)lureandcaptureinsectpreyusingstickymucilageofleaftentacles,in
order to obtain extra nutrients since growing in nutrientpoor peat bogs. In this work, the
effectsofdifferentgrowthconditionsonphotosyntheticefficiency,contentofphotosynthetic
pigments and phenolic compounds, and antioxidative activity of leaf blades and petioles of
DroseraspatulataandD.aliciaewereinvestigated.Onesetofsundewswasgrownindoorsat
lowlightintensitieswithscarcepreyavailability.Anothersetofplantswasgrownoutdoorsat
high light intensities with greater insect availability. In general, leaf blades performed higher
photosynthetic efficiency and contained more photosynthetic pigments and phenolic
compoundsthanpetiolesofanalyzedspecies.Despiteofincreasedcontentofchlorophyllsand
carotenoids in indoorgrowing plants, the p hotosynthetic efficiency was higher in outdoor
growingplants,probablyduetohighlightintensitiesexposureand/orgreaterpreyavailability.
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The contents of flavonoids and anthocyanins were higher in leaf blades of outdoorgrowing
plants, resulting in increased content of total phenolic compounds in D. spatulata. On the
contrary,D.aliciaeplantscontainedmorephenoliccompoundsinindoorgrowingconditions.
Antioxidative activity was high in blade and petiole extracts of investigated species grown in
both indoor and outdoor conditions, with exception of petiole extract of D. spatulata grown
indoors.
Keywords:Droseraceae,photosynthesis,chlorophylls,phenoliccompounds
P80
ZAJEDNIKERAZVOJNEZNAAJKESOMATSKEIZIGOTNEEMBRIOGENEZE
D.LeljakLevani1,N.Bauer1,S.Mihaljevi2
1
Zavod za molekularnu biologiju, Bioloki odsjek, Prirodoslovnomatematiki fakultet,
SveuiliteuZagrebu,Zagreb,Hrvatska(dunja.leljaklevanic@biol.pmf.hr)
2
Zavodzamolekularnubiologiju,InstitutRuerBokovi,Zagreb,Hrvatska
Kljunerijei:somatskaembriogeneza,zigotnaembriogeneza,metilacijaDNA
SOMATICANDZYGOTICEMBRYOSSHARECOMMONDEVELOPMENTALFEATURES
D.LeljakLevani1,N.Bauer1,S.Mihaljevi2
1
Division of Molecular Biology, Department of Biology, Faculty of Science, University of
Zagreb,Zagreb,Croatia(dunja.leljaklevanic@biol.pmf.hr)
2
DepartmentofMolecularBiology,RuderBokoviInstitute,Zagreb,Croatia
Inmanyvascularplants,zygoticreproductionregularlyalternateswithdifferenttypesofasexual
reproduction,soembryoscandevelopnotonlyfromfertilizedeggcells,butalsofrominduced
somatic cells through the process of somatic embryogenesis. Although somatic and zygotic
embryogenesis are not directly correlated, their common features will be presented,
demonstrating that the origin and development of the somatic embryo morphologically and
physiologically resembles zygotic embryogenesis at certain points. In order to initiate
embryogenesis,bothcompetenteggandsomaticcellsrequireactivationeitherbyfertilizationor
by specific environmental signals respectively. During induction of somatic and zygotic
embryogenesis modulation of DNA methylation, activation of particular hormonal and stress
related mechanisms including changes in cell wall propertie s are triggered. We will give an
overview and discuss the recent knowledge in the field of plant somatic and zygotic
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12.HBK21712 CBC
embryogenesis, with special attention given to the onset of embryogenesis and early embryo
development as well as to embryogenesisrelated interconnections between plant hormones,
stressresponses,DNAmethylationandregulatorygeneexpression.
Keywords:somaticembryogenesis,zygoticembryogenesis,DNAmethylation
P81
RASPROSTRANJENOST DRVENASTIH INVAZIVNIH VRSTA Robinia pseudoacacia L.,
ReynoutriajaponicaHoutt.IAcernegundoL.NAPODRUJUSAMOBORA
N.Kleteki1,T.Vujnovi2,E.Kleteki3,M.Vukovi3
1
OBogumilaTonija,Samobor,Hrvatska(natasa.kletecki@gmail.hr)
2
Dravnihidrometeorolokizavod,Sektorzahidrologiju,Zagreb,Hrvatska
3
Hrvatskiprirodoslovnimuzej,Zagreb,Hrvatska
Kljunerijei:invazivnaflora,Samobor,Hrvatska
DISTRIBUTIONOFNONHERBACEOUSINVASIVESPECIESRobiniapseudoacaciaL.,
ReynoutriajaponicaHoutt.ANDAcernegundoL.INTHESAMOBORAREA
N.Kleteki1,T.Vujnovi2,E.Kleteki3,M.Vukovi3
1
'BogumilToni'Primaryschool,Samobor,Croatia(natasa.kletecki@gmail.com)
2
Meteorologicalandhydrologicalservice,HydrologySector,Zagreb,Croatia
3
CroatianNaturalHistoryMuseum,Zagreb,Croatia
This work brings results of the research into distribution of nonherbaceous invasive species
Robinia pseudoacacia L., Reynoutria japonica Houtt. and Acer negundo L. in urban parts of the
townofSamoborduringvegetationseasonsof2010and2011.ForeachmarkedGPSpositionwe
determined the habitat type according to the National Habitat Classification of Croatia. We
recorded altogether 31 habitats of allochthonous nonherbaceous plants. The species Acer
negundoL.hadthegreatestnumberofhabitats16,andReynoutriajaponicaHoutt.hadonly3.
Acer negundo L. also has the biggest portion of habitats in the total number of all recorded
allochthonous nonherbaceous plant habitats 51%. It is followed by Robinia pseudoacacia L.
foundin39%andReynoutriajaponicaHoutt.foundinonly10%ofrecordedhabitats.
Keywords:invasiveflora,Samobor,Croatia
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P82
ULOGATRANSPORTERAHMAUAKUMULACIJICdUVRSTINicotianaglauca
K.Majsec,M.Pavlica
Zavodzamolekularnubiologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuilite
uZagrebu,Zagreb,Hrvatska(kmajsec@biol.pmf.hr)
Kljunerijei:hiperakumulacijaCd,Nicotianaglauca,transporteriHMA
ROLEOFHMATRANSPORTERSINCdACCUMULATIONINNicotianaglauca
K.Majsec,M.Pavlica
DivisionofMolecularBiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(kmajsec@biol.pmf.hr)
12.HBK21912 CBC
Keywords:Cdhyperaccumulation,Nicotianaglauca,HMAtransporters
P83
HRANIDBENA VRIJEDNOST KORE, PLODOVA I SJEMENKI OSKORUE (Sorbus domestica L.):
MINERALIIFENOLNISPOJEVI
I.ola1,B.Maji1,2,S.Liki1,I.JuranoviCindri2,G.Rusak1
1
Botaniki zavod, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska(ivana.sola@biol.pmf.hr)
2
Zavodzaanalitikukemiju,Kemijskiodsjek,Prirodoslovnomatematikifakultet,Sveuiliteu
Zagrebu,Zagreb,Hrvatska
Ciljovogradabiojei)istraitisadrajanorganskih(makroimikroelemenata)iorganskihtvari
(ukupnifenoli,flavonoidiitanini)teii)antioksidativnuaktivnostukupnihfenolaitaninaizkore,
plodova(odvojenoegzokarpimezokarp)isjemenkivrsteS.domesticaL.koristeifitokemijske
analize. Rezultati su pokazali da su svi testirani uzorci bogati kalijem. Kora se pokazala kao
najbolji izvor kalcija i cinka, dok su sjemenke najbolji izvor magnezija. Ekstrakti nezrelog
egzokarpaikoresadrenajveukoliinuukupnihfenola.Zrenjemsekoliinafenolnihspojevau
plodovima(iegzokarpu i mezokarpu) znaajno smanjila. Egzokarp je znatno bogatijiukupnim
flavonoidima nego mezokarp, kora i sjemenke. Najbolju antioksidativnu aktivnost pokazali su
ukupni fenoli nezrelog egzokarpa i kore. Antioksidativna aktivnost ukupnih fenola i tanina
egzokarpa, ali ne i mezokarpa, znaajno se smanjila nakon zrenja. Na temelju dobivenih
podataka zakljuujemo da su kora i sjemenke oskorue znaajno bolji izvori makro i
mikroelemenatanegoplodovi,doksuegzokarpikoraboljiizvoriukupnihfenolaiflavonoida.
Predlaemo nedovoljno koritenu vrstu Sorbus domestica L. kao odlian izvor minerala i
antikosidativnihtvarivanihuljudskojprehrani.
Kljunerijei:makroimikroelementi,flavonoidi,tanini,antioksidativnaaktivnost
NUTRITIONAL VALUE OF Sorbus domestica L. BARK, FRUITS AND SEEDS: MINERALS AND
PHENOLICS
I.ola1,B.Maji1,2,S.Liki1,I.JuranoviCindri2,G.Rusak1
1
Division of Botany, Department of Biology, Faculty of Science, University of Zagreb, Zagreb,
Croatia(ivana.sola@biol.pmf.hr)
2
Division of Analytical Chemistry, Department of Chemistry, Faculty of Science, University of
Zagreb,Zagreb,Croatia
Theaimofthisstudywastoi)investigatethecontentofinorganic(macroandmicroelements)
andorganiccompounds(totalphenolics,totalflavonoidsandtotaltannins)andii)evaluatethe
antioxidant activities of total phenolics and total tannins in S. domestica L. bark, fruits
(separatelyexocarpandmesocarp)andseedsusingphytochemicalanalyses.Resultsrevealed
all the tested service tree samples were rich in potassium. Bark, among the tested samples,
was the best source of calcium and zinc, while seeds were the best source of magnesium.
Extracts of immature exocarp and bark contained the highest amounts of total phenolics.
Ripening significantly decreased the amount of phenolics in fruits (both exocarp and
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12.HBK22012 CBC
mesocarp). Exocarp was significantly richer in total flavonoids than mesocarp, bark or seeds.
Thehighestantioxidantactivityshowedphenolicsfromimmatureexocarpandthenfrombark.
The antioxidant activity of phenolics and tannins from exocarp, but not from mesocarp, was
significantly decreased after ripening. Based on the data we conclude that service tree bark
and seeds are significantly better sources of macro and microelements than fruits, while
exocarpandbarkarebettersourcesoftotalphenolsandflavonoids.Wesuggestthatunder
utilizedspeciesSorbusdomesticaL.couldbeusedasagreatsourceofmineralelementsand
antioxidantsimportantforhumandiet.
Keywords:macroandmicroelements,flavonoids,tannins,antioxidantactivity
P84
FOTOSINTETSKA UINKOVITOST, UDIO FOTOSINTETSKIH PIGMENATA I FENOLNIH SPOJEVA
TEANTIOKSIDACIJSKAAKTIVNOSTURAZLIITIMDIJELOVIMAVREVAUTRIHIBRIDARODA
Sarracenia
M.Tuek,M.Curman,M.Babi,M.Tkalec
Sveuilite u Zagrebu, Prirodoslovnomatematiki fakultet, Bioloki odsjek, Zagreb, Hrvatska
(martina.tusek@hotmail.com)
Kljunerijei:Sarracenia,klorofili,karotenoidi,fenolnispojevi,fotosinteza
M.Tuek,M.Curman,M.Babi,M.Tkalec
University of Zagreb, Faculty of Science, Department of Biology, Zagreb, Croatia
(martina.tusek@hotmail.com)
Sarracenia is a genus of carnivorous plants known by leaves modified into pitchers which
lore,huntanddigestinsects.Inthisstudy,photosyntheticefficiency,contentofchlorophylls,
carotenoids and phenolic compounds, and antioxidant activity in different parts of the
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12.HBK22112 CBC
pitcher (wing, operculum, pitcher upper and lower) in three Sarracenia hybrids were
analyzed. Hybrid A has small pitcher opening, operculum in which opposite edges merge,
andredveinsonoperculumandpitcherupperpart.HybridBisthelargestofthreehybrids
andhasoperculumwithredveins.HybridCisthesmallest,withredcoloredoperculumand
pitcherupperpart.Resultsindicatethatthecolorationofpitcherpartsisrelatedtoamount
of phenolic compounds and photosynthetic pigments; the redcolored operculum and the
upper part contain higher amount of phenolic compounds, while greencol ored wing and
the lower part contain higher amount of chlorophylls and carotenoids. Analysis of
photosyntheticparametersshowslowerphotosynthesisrateinoperculumandpitcherupper
partofhybridB.HybridBalsohashigheramountofchlorophyllandcarotenoidsinwingand
pitcher lower part. In conclusion, decline in photosynthetic efficiency in pitcher upper part
andoperculumiscausedbyloweramountofchlorophyll.Althoughtheoperculumcontains
the highest amount of phenolic compounds and flavonoids, especially in hybrid C, the
extractsofallpitcherpartsshowhighantioxidantactivity.
Keywords:Sarracenia,chlorophylls,carotenoids,phenoliccompounds,photosynthesis
P85
ANTIOKSIDACIJSKA,ANTIBAKTERIJSKAICITOTOKSINAAKTIVNOSTVODENOGEKSTRAKTA
DUBROVAKEZEINE(CentaurearagusinaL.)
V.Vuji1,S.RadiBrkanac1,M.RadiStojkovi2,S.Ivankovi3,J.Hrenovi4,M.Rui5,
B.PevalekKozlina1
1
Botanikizavod,Biolokiodsjek,Prirodoslovnomatematikifakultet,SveuiliteuZagrebu,
Zagreb,Hrvatska(valerija.vujcic@biol.pmf.hr)
2
Zavodzaorganskukemijuibiokemiju,InstututRuerBokovi,Zagreb,Hrvatska
3
Zavodzamolekularnumedicinu,InstututRuerBokovi,Zagreb,Hrvatska
4
Zavod za mikrobiologiju, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u
Zagrebu,Zagreb,Hrvatska
5
Odjelzabiologiju,Prirodoslovnomatematikifakultet,SveuiliteuSplitu,Split,Hrvatska
Dubrovakazeina(CentaurearagusinaL.)jeendeminahrvatskabiljnavrstabogatabioaktivnim
spojevima.Ciljovogistraivanjajeodreditisadrajpolifenolauvodenimekstraktimabiljnevrste
C.ragusinasakupljenenaprirodnomlokalitetu(KatalinibrigiSustipan)iuzgojeneukulturitkiva
kao i njihovu antioksidacijsku, antibakterijsku i citotoksinu aktivnost. U svrhu razjanjenja
bioloke aktivnosti prouavane su interakcije vodenih ekstrakata s polinukleotidima. Najvii
sadraj ukupnih fenola, flavonoida, flavonola, hidroksicimetnih kiselina i proantocijanidina
izmjeren je u vodenim ekstraktima kalusa uzgojenim u uvjetima in vitro. Gotovo svi ispitani
ekstrakti pokazali su neznatnu antibakterijsku aktivnost na Acinetobacter baumannii i
Staphylococcus aureus, umjerenu antioksidacijsku aktivnost kao i znaajnu citototoksinu
aktivnostnastaninimlinijamaSCCVII,FsaR,B16F10iV79.Interakcijamavodenihekstrakatas
polinukleotidima (ctDNA, poli A poli U) mjerenim UVVIS spektroskopijom, promjenom
temperature mekanja polinukleotida (DTm) te spektroskopijom cirkularnog dikroizma (CD
spektroskopijom) utvren je znaajan utjecaj vodenih ekstrakata na stabilnost ispitivanih
polinukleotidakaoinanjihovukonformaciju.
Kljunerijei:CentaurearagusinaL.,fitokemikalije,biolokaaktivnost,interakcijespolinukleotidima
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12.HBK22212 CBC
V.Vuji1,S.RadiBrkanac1,M.RadiStojkovi2,S.Ivankovi3,J.Hrenovi4,M.Rui5,
B.PevalekKozlina1
1
Division of Botany, Department of Biology, Faculty of Science, University of Zagreb, Zagreb,
Croatia(valerija.vujcic@biol.pmf.hr)
2
DivisionofOrganicChemistryandBiochemistry,RudjerBoskovicInstitute,Zagreb,Croatia
3
DivisionofMolecularMedicine,RudjerBoskovicInstitute,Zagreb,Croatia
4
Division of Microbiology, Department of Biology, Faculty of Science, University of Zagreb,
Zagreb,Croatia
5
DepartmentofBiology,FacultyofScience,UniversityofSplit,Split,Croatia
CentaurearagusinaL.isanendemicCroatianplantspeciesrichinbioactivesubstances.Thegoalof
thisworkistodeterminepolyphenolcontentinwaterextractsofC.ragusinacollectedatnatural
habitats (Katalini brig i Sustipan) and cultivated in vitro as well as to estimate their antioxidant,
antibacterial and cytotoxic activity. The interactions of extracts with double stranded
polynucleotideswerestudiedtoelucidatethebiologicalactivityofC.ragusina.Thehighestlevelof
totalphenolics,flavonoids,flavonols,hydroxycinnamicacidsandproanthocyanidinsweremeasured
incalliwaterextractscultivatedinvitro.Almostalltestedextractsshowedweakantibacterialactivity
againstAcinetobacterbaumanniiandStaphylococcusaureus,moderateantioxidantactivityaswell
as significant cytotoxic activity on cell lines SCCVII, FsaR, B16F10 and V79. Interactions of water
extractswithpolynucleotides(ctDNA,polyApolyU)measuredbyUVVISspectroscopy,melting
experiments (DTm) and circular dichroism (CD) spectroscopy showed significant impact of water
extractsonthestabilityofstudiedpolynucleotidesandontheirconformation.
Keywords:CentaurearagusinaL.,phytochemicals,biologicalactivity,interactionswithpolynucleotides
P86
DOSUGARCONTENTSINSELECTEDMOSSSPECIESIMPLYTOTHEIRSALTRESISTANCE?
M.Vujii1,M.Sabovljevi1,M.osi1,V.Maksimovi2,D.Mii3,A.Sabovljevi1
1
Bryophyte Biology Group Belgrade, Institute of Botany and Botanical Garden, Faculty of
Biology,UniversityofBelgrade,Belgrade,Serbia
2
InstituteforMultidisciplinaryResearch,UniversityofBelgrade,Belgrade,Serbia
3
InstituteforBiologicalResearch,UniversityofBelgrade,Belgrade,Serbia
Mosses are the first plants that face the terrestrial way of living and the harsh environment
conditions. Some species can be also recorded in brackish water or salty soils, i.e. facultative
halophytes.Forstudypurposeoftheresistance,toleranceandadaptationtosalt,wechosethree
mossspecieswithsimilarlifespanandlifecycle,namely:Physcomitrellapatens(nonhalophytic
control moss), Entosthodon hungaricus and Henediella heimii (two facultative halophytic
mosses). The in vitro cultures were established and growth conditions optimized prior to
experimentation.Insuchaconditionsmossplantsweretreatedwithdifferentconcentrationof
NaCl(0500mM)forthreeweekspriortoanalyzingsugarcontentbyHPLC.Itwasshownthatall
specieshavehighlevelofsucrose(upto350l/mL),glucose(upto280l/mL)andfructose(up
to400l/mL)intotalsugarprofiles.However,sugarcontentsvarydifferentlyintwohalophyte
mosses.H.heimiiseemstohavepredefinedsucrose(ca.200l/mL)contentwhichtendtobe
maintainedduringincreasesaltstress,whileE.hungaricusreacttosaltstressinquicksynthesisof
fructose and glucose. The sugar level in E. hungaricus is predefined only for low level of salt
stress,whichpostponevigorousreactiontomediumandhighsaltexposure.However,P.patens
reactsimmediatelyuponsaltstress(5mM)byincreaseofinitiallylowlevelofsucrose,fructose
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Keywords:bryophytes,halophytes,saltstress,sucrose,fructose,glucose
P87
UINAKNANOESTICASREBRANAPOJAVUOKSIDACIJSKOGSTRESAUKLIJANCIMADUHANA
(Nicotianatabacum)
M.Zovko1,P.Cvjetko2,I.VinkoviVrek3,B.Balen2
1
Agencijazazatituokolia,Zagreb,Hrvatska
2
Zavod za molekularnu biologiju, Bioloki odsjek, Prirodoslovnomatematiki fakultet,
SveuiliteuZagrebu,Zagreb,Hrvatska(bbalen@biol.pmf.hr)
3
Jedinicazaanalitikutoksikologijuimineralnimetabolizam,Institutzamedicinskaistraivanjai
medicinurada,Zagreb,Hrvatska
Kljunerijei:duhan,nanosrebro,oksidacijskistres
M.Zovko1,P.Cvjetko2,I.VinkoviVrek3,B.Balen2
1
CroatianEnvironmentAgency,Zagreb,Croatia
2
DivisionofMolecularBiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(bbalen@biol.pmf.hr)
3
Analytical Toxicology and Mineral Metabolism Unit, Institute for Medical Research and
OccupationalHealth,Zagreb,Croatia
Nanomaterials are widely used in everyday life, and silver nanoparticles (AgNPs) are often
applied.Theyprovidebetterutilizationofantibacterialandantifungaleffectsofsilver.Numerous
studiesconductedtodeterminetheirtoxiceffectonecosystems,andthereforeonplants,arein
thefocusofinterest.Tobaccoisamodelplantoftenusedinabioticstressstudies.Inthisresearch
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the effects of AgNPs on tobacco seedlings were analyzed. Seedlings were also exposed to the
sameconcentrationsofionicsilver(AgNO3).Fortheevaluationofoxidativestressthecontentof
malondialdehyde (MDA) and protein carbonyls as well as the activity of antioxidative enzymes
pyrogallol (PPX), ascorbate peroxidase (APX) and catalase (CAT) were analyzed
spectrophotometricallyandingel.Comparedtocontrol,allAgNO3treatmentsledtoadecrease
ofMDA,andthesametrendwasobservedafterexposuretothehigherAgNPconcentrations.
TheincreaseofcarbonylswasrecordedinseedlingstreatedwithAgNPs,whileexposuretoAgNO3
showed no change. Generally, PPX and CAT activity was reduced after the AgNP and AgNO3
treatments, although the decrease of PPX activity was more expressed in AgNO3 treatments.
Increased activity of APX was determined in all treatments. The results revealed that oxidative
stressparticipatesinthetoxicitymechanismofAgNPsandsuggeststhatinvolvementofplantsin
thecomprehensiveresearchofnanoparticlestoxicityisconvenientandnecessary.
Keywords:tobacco,nanosilver,oxidativestres
3.SIMPOZIJHRVATSKOGENTOMOLOKOGDRUTVA
3rdSYMPOSIUMOFTHECROATIANENTOMOLOGYSOCIETY
P88
USPOREDBA UINKOVITOSTI METODA UZORKOVANJA VODENIH KUKACA (HETEROPTERA I
COLEOPTERA)SOBZIROMNAATRAKTANT,VELIINUVRSTAISTANITE
M.Bistrovi1,N.Turi1,M.Temunovi2,G.Vignjevi1,E.Merdi1
1
SveuiliteJ.J.StrossmayerauOsijeku,Odjelzabiologiju,Osijek,Hrvatska(matea.bistrovic@gmail.com)
2
SveuiliteuZagrebu,umarskifakultet,Odjelzaumarskugenetiku,dendrologijuibotaniku,
Zagreb,Hrvatska
Kljunerijei:vodenikukci,uinkovitostiselektivnostmetodauzorkovanja,poplavnopodruje,
melioracijskikanali
M.Bistrovi1,N.Turi1,M.Temunovi2,G.Vignjevi1,E.Merdi1
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12.HBK22512 CBC
Although various methods exist for sampling aquatic insects (Heteroptera and Coleoptera),
comparisonsoftheirperformanceacrossdifferenthabitattypesarestillscarce.Inthisstudy,
weevaluatedandcomparedtheefficiencyofthreesamplingmethods:handnet(HN),bottle
trapswithcannedtunafish(BTF)andbottletrapswithcannedcatfood(BCF).Theaimofour
investigationwastodeterminetheefficiencyandselectivityofsamplingmethodsindifferent
habitattypesandalsoefficiencyofnewlyintroducedbaitcannedtunafishinastudyofaquatic
insects. The methods were compared over the period 2010 2012 in two different habitat
types,meliorationcanalsandfloodedareas,intheNatureParkKopakirit,afloodplainareaof
the Danube River. The results show that the habitat type has a major influence on the
effectiveness of the method. Overall, handnetting was proven to be the most successful
method, primarily at melioration canals. Canned tuna fish bait was more efficient than the
commonlyusedcatfood,especiallyforlargeandhighlymobileDytiscidaespecies.Ourresults
reinforcetheneedforlongtermsamplingcombinedwithmultiplecomplementarymethodsto
estimatetheoverallspeciesrichnessofthearea.
Keywords:aquaticinsects,samplingefficiencyandselectivity,floodplain,canals,baits
P89
RASPROSTRANJENOST CRNE UDOVICE (Latrodectus tredecimgutattus, Rossi 1790.) NA
PODRUJUHRVATSKEIBIH
N.Landeka1,I.upi2
1
ZavodzajavnozdravstvoIstarskeupanije,Pula,Hrvatska(ddd@zzjziz.hr)
2
Prirodoslovnomatematikifakultet,Zagreb,Hrvatska
Crnuudovicu,najotrovnijegeuropskogpauka,napodrujuHrvatskenalazimouIstri,Primorjui
kvarnerskimotocima,teurazliitimdijelovimaDalmacije.UBiHjerasprostranjenanapodruju
juneizapadneHercegovine.Krozrazdobljeod2011.do2014.godinepretraenasurazliita
stanita na temelju literaturnih podataka, razliitih podataka iz medija i drugih izvora te na
temelju pretpostavke njihovog prisustva (krki travnjaci, klimatske znaajke, kamenjarski
travnjacinavisoravnima).Natemeljudobivenihpodatakaizraenajekartarasprostranjenosti
napodrujuHrvatskeiBiH.Kakotijekomrazdobljaistraivanjanijebilomasovnihgradacijana
mnogim lokacijama jedinke su bile malobrojne a tako i teko uoljive. Zbog toga kartu ne
moemosmatratiikonanom.
Kljunerijei:crnaudovica,kartarasprostranjenosti,stanita
DISTRIBUTIONOFTHEBLACKWIDOWSPIDER(Latrodectustredecimgutattus,Rossi1790.)IN
THEAREAOFCROATIAANDBOSNIAANDHERZEGOVINA
N.Landeka1,I.upi2
1
PublicHealthInstituteoftheIstrianRegion,Pula,Hrvatska(ddd@zzjziz.hr)
2
FacultyofScience,Zagreb,Hrvatska
InCroatia,theblackwidowspider,whichisknownasthemostpoisonousEuropeanspider,can
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12.HBK22612 CBC
befoundinIstria,costalareaandtheKvarnerianislands,aswellasindifferentpartsofDalmatia.
InBosniaandHerzegovinathespeciescanbefoundintheareasofsouthandwestHerzegovina.
Throughouttheperiodfrom2011to2014,differenthabitatshavebeenresearchedbasedonthe
information from reference data and other sources including media and assumptions of the
occurrence of the species (karst grasslands, climate). Based on the gathered data a map was
created showing the distribution of the black widow spider in Croatia and Bosnia and
Herzegovina.Astherewasnomassivegradationonmanyofthelocationstroughtheperiodof
researchtheindividualswerefewandhardtospot.Thereforewecannotconsiderthemapfinite.
Keywords:blackwidowspider,mapofdistribution,habitats
P90
FAUNASKAKAVACA(INSECTA:ORTHOPTERA:CAELIFERA)HRVATSKE
J.Skejo
UdrugastudenatabiologijeBIUS,Zagreb,Hrvatska(skejo.josip@gmail.com)
Fauna skakavaca (Orthoptera: Caelifera) Hrvatske nikad nije sistematino istraivana, a jedini
popisvrstakojipostojipotjeeizvremenaJugoslavijeisadrimnogopodatakakojejepotrebno
raspraviti.Ipak,podatcioskakavcimaHrvatskerasprenisuumnogobrojnimpublikacijama,a
jedinkepomnogimmuzejima.Ciljetverogodinjegistraivanja(2011.2015.)biojeobjediniti
sveliteraturneidostupnemuzejskepodatkenajednomjestotenanjihdatikritikiosvrt,au
isto vrijeme znanje o ovoj zanemarenoj skupini kukaca nadopuniti novim terenskim i
eksperimentalnim podatcima. Sakupljeno je preko 200 radova o fauni skakavaca Hrvatske, u
suradnjisastranimekspertimaikustosimaeuropskihmuzejaobjedinjenisudostupnimuzejski
podatci,odraenojeprekotridesetterenadiljemHrvatske,auzorcisuprepariraniistvorenaje
nova mokra i suha zbirka skakavaca Hrvatske. Predstavljena je fauna skakavaca Hrvatske.
Sveukupno,uHrvatskojjedosadazabiljeeno85vrstai3podvrsteskakavaca.Predstavljenje
broj vrsta po regijama (panonska, dinarska, mediteranska), raspravljeni su nalazi upitnih,
rijetkihizanimljivihvrsta.Predloenojepreko15sinonimaipreko10taksonomskihpromjena.
Predstavljenisutakoerinekizanimljivirezultatibioakustikihanalizatesudaniprijedloziza
daljnjaistraivanjataksonomije,nomenklature,ekologije,etologije,bioakustikeibiogeografije
ravnokrilaca.
Kljunerijei:faunistika,taksonomija,ravnokrilci,popis,revizija
CROATIANSHORTHORNEDGRASSHOPPERS'(INSECTA:ORTHOPTERA:CAELIFERA)FAUNA
J.Skejo
BiologystudentsassociationBIUS,Zagreb,Croatia(skejo.josip@gmail.com)
Croatian Caeliferan fauna (Orthoptera) has never been systematically investigated, the only
species'checklistoriginatingfromYugoslaviaandcontainsalotofdoubtfuldata.However,the
data on Croatian shorthorned grasshoppers are scattered within a lot of publications, while
specimensaredepositedwithinalotofmuseums.Theaimofthe4yearsresearch(20112015)
ofthisgroupwastogatheralltheliteratureandmuseumsdataandtogivecriticalreview,and
inthesametimetofulfiltheexistingdatawithnewfieldworkandexperimentalinformation.
More than 200 papers about Croatian Caeliferan fauna were gathered, via cooperation with
foreignexpertsandmuseumcuratorsdatafromEuropeanmuseumswerecollected,aswell,
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morethan30fieldworksalloverCroatiahavebeenconductedthisbeingthefundamentfor
newwetanddrycollectionofCroatianCaelifera.FaunaoftheCroatianCaeliferaispresented.
Hitherto, altogether 85 species and 3 additional subspecies have been recorded for Croatian
fauna. Number of the species by region (Pannonian, Dinaric, and Adriatic) is presented;
doubtful,newandinterestingdataarediscussed.Morethan15synonymsand10taxonomical
actsareproposed.Someinterestingbioacousticalresultsarepresentedaswell.Proposalsand
plans for future research on Orthopteran taxonomy, nomenclature, ecology, ethology,
bioacousticsandbiogeographyareintroduced.
Keywords:faunistics,taxonomy,grasshoppers,checklist,review
P91
RAZNOLIKOSTVRETENACAHRVATSKOGAZAGORJA
A.tih,T.Koren
HrvatskoherpetolokodrutvoHyla,Zagreb,Hrvatska(ana.stih2@gmail.com)
FaunavretenacaHrvatskogazagorjanikadanijebilapredmetsustavnihfaunistikihistraivanja,
sukladnotomesupodatciofaunivretenacaoveregijegotovonepostojei.Uprilogtomegovori
injenica da je do sada u fauni Hrvatske zabiljeeno 75 vrsta vretenaca, dok je pregledom
dostupne literature, za podruje Hrvatskog zagorja utvreno samo njih 13. Budui da Hrvatsko
zagorje obiluje mnotvom vodenih tijela te zauzima veliki dio sjeverne Hrvatske, broj poznatih
vrstajedalekoodstvarnogbrojavrstaprisutnihnatomepodruju.Tijekom2012.i2013.godine
provedenojeprvosustavnoistraivanjefaunevretenacaHrvatskogzagorja.Utvrenesu34vrste
odegasu23vrstepoprviputazabiljeenezapodrujeHrvatskogzagorja.Sedampronaenih
vrsta nalazi se u Crvenoj knjizi vretenaca Hrvatske, dok je jedna vrsta na Natura 2000 popisu.
Nakon ovoga istraivanja Hrvatsko zagorje postaje jedno od najbolje istraenih podruja u
Hrvatskojtosetiefaunevretenaca,sukupno37zabiljeenihvrsta.
Kljunerijei:vretenca,Hrvatskozagorje,raznolikost,Natura2000
DIVERSITYOFDRAGONFLYFAUNAOFHRVATSKOZAGORJE
A.tih,T.Koren
CroatianHerpetologicalSocietyHyla,Zagreb,Croatia(ana.stih2@gmail.com)
Dragonfly fauna of Hrvatsko zagorje has never been systematically studied and accordingly
literature data about dragonfly fauna of mentioned region are almost nonexistent. This is
corroboratedbythefactthat75dragonfliesspeciesaresofarknownfromCroatia,whilefor
theHrvatskozagorje13specieshavebeenrecordedintheliterature.SinceHrvatskozagorjeis
richinwaterbodiesandtakesupalargepartofthenorthernCroatia,thenumberofknown
speciesisfarfromtheexpectednumberofspeciespresentinthatarea.Duringtheyears2012
and2013weconductedthesurveyofdragonfliesanddamselfliesfauna(Insecta,Odonata)of
Hrvatsko zagorje. Thirty four species were found, of which 23 species were recorded for the
firsttimefortheregion.SevenspeciesarelistedintheReddatabookofdragonfliesofCroatia,
whileonespeciesisonNatura2000list.AfterthissurveyHrvatskozagorjebecomesoneofthe
bestsurveyareasasregardsdragonflyfauna,withatotalof37recordedspecies.
Keywords:Dragonfly,Hrvatskozagorje,diversity,Natura2000
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12.HBK22812 CBC
P92
NOVE VRSTE TULARA (INSECTA: TRICHOPTERA) ZA FAUNU HRVATSKE IZ PARKA PRIRODE
KOPAKIRIT
I.Vruina1,A.Previi2,E.Merdi1
1
Odjel za biologiju, Sveuilite Josipa Jurja Strossmayera u Osijeku, Osijek, Hrvatska
(iboca@biologija.unios.hr)
2
Biolokiodsjek,Prirodoslovnomatematikifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
Odraslitulari(Trichoptera)prikupljanisuurazdobljuod2010.2012.godinenapodrujuParka
prirode Kopaki rit na ukupno 8 postaja. Ovo su prva istraivanja faune i ekologije tulara na
podrujuparkakojasuobuhvatilarazliitetipoveslatkovodnihstanita.Uzorkovanjasuobavljena
pomou svjetlosne klopke (UV lampe BLB 15 W) jednom mjeseno, izuzev svibnja kada je
uzorkovanje obavljeno dva puta. Dvije vrste zabiljeene ovim istraivanjem nove su za faunu
Hrvatske: Oecetis furva i Ortotrichia tragetti. Obje su vrste zabiljeene na razliitim tipovima
stanita(rijenitok,jezero,kanali).VrstaOecetisfurvazabiljeenajenadonjemtokurijekeDrave,
zatim na eutrofnom jezeru (Sakadakom jezeru), povremenom vodotoku s bazeniima
(Vemeljski dunavac) i kanalu s povremenim protokom (kanal arna). Vrsta Ortotrichia tragetti
zabiljeenajenaeutrofnomjezeru(Sakadakomjezeru)ikanaluspovremenimprotokom(kanal
arna).OvipodatcipredstavljajuznaajandoprinospoznavanjufaunetularaHrvatskeopenito,a
posebicenedovoljnoistraenihmovarnihijezerskihstanitakontinentalnogdijelaHrvatske.
Kljunerijei:Trichoptera,Oecetisfurva,Ortotrichiatragetti,movarnastanita,jezera
NEW SPECIES OF CADDISFLIES (INSECTA: TRICHOPTERA) FOR CROATIAN FAUNA FROM THE
KOPAKIRITNATUREPARK
I.Vruina1,A.Previi2,E.Merdi1
1
Department of Biology, J.J. Strossmayer University of Osijek, Osijek, Croatia
(iboca@biologija.unios.hr)
2
DepartmentofBiology,FacultyofScience,UniversityofZagreb,Zagreb,Croatia
Adultcaddisflies(Trichoptera)werecollectedfrom2010to2012intheKopakiRitNaturePark.
Collecting was conducted on a total of 8 collecting sites encompassing various freshwater
habitats.Thecurrentstudyisthefirststudyoffaunisticsandecologyofcaddisfliesinthepark.
Samplingwasconductedusinglighttraps(UVlampBLB15W)thatwereoperatedmonthly,with
the exception of May when it was carried out on two occasions. Two species collected in this
studyarenewfortheCroatianfauna:Oecetisfurva,andOrtotrichiatragetti.Bothspecieswere
collected at various habitats (river course, lake, channels). Oecetis furva was collected at the
lower reach of the Drava River, in the eutrophic lake (Sakadako Lake), and Vemeljski dunavac
andarnachannels.Ortotrichiatragettiwascollectedattheeutrophiclake(SakadakoLake)and
arna channel. These data represent a significant contribution to the general knowledge of
caddisflies in Croatia, and particularly of insufficiently investigated wetlands and lakes in the
continentalregion.
Keywords:Trichoptera,Oecetisfurva,Ortotrichiatragetti,wetlands,lakes
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BIOLOGIJAKOPNENIHVODAIKOPNA
BIOLOGYOFFRESHWATERANDTERRESTRIALECOSYSTEMS
P93
STRUKTURA ZAJEDNICE I HRANIDBENE NAVIKE SLATKOVODNIH RIBA U PLITKOM JEZERU
UMJERENOGPODRUJA
Ihtiocenozepredstavljajujedanodkljunihelemenataufunkcioniranjulentikihekosustava,
osiguravajuiprotokenergijeiprocesrecikliranjanutrijenata.Istraivanjesastavaistrukture
ihtiocenoze provedeno je tijekom srpnja 2014. godine u Kopakom jezeru, Park prirode
Kopaki rit. Ispitan je sastav probavila riba kako bi se utvrdila vanost pojedinih kategorija
hrane u njihovoj ishrani, s obzirom da ribe svojom ishranom mogu utjecati na bentos i
obrataj.Uzorkovanjejeprovedenopomoutrostrukepoponice,postavljenetijekomveeri
da stoji 12 sati, koja je potom izvuena. Uzorkovani primjerci riba fiksirani su u 4%tnom
formaldehidu radi kasnije obrade u laboratoriju. Ribolovni napor (CPUE) iznosio je 2,71
kg/mreasati. Tijekom istraivanja su uzorkovane 143 jedinke, starosti izmeu 1+ i 6+
godina. Utvreno je 9 razliitih vrsta koje pripadaju porodicama: Esocidae, Cyprinidae i
Percidae. Najbrojnija vrsta je bila Blicca bjoerkna, s uzorkovanih 50 jediniki. Takoer,
zabiljeenjeveibrojmujakanegoenki.Uprobavilimaribautvrenesurazliitekategorije
hrane: vegetacija, detritus, beskraljenjaci, uz dominaciju liinki kukaca, te ribe. Prema
sastavu probavila, uzorkovane ribe moemo klasificirati kao omnivore, bentivore te
piscivore. Takoer, pronaeni su i endoparaziti u jedinkama vrsta B. bjoerkna, Alburnus
alburnus i Gymnocephalus baloni. Zabiljeena ihtiofauna karakteristina je za podruje i
staniteistraivanja,autvrenehranidbenenavikeukazujunarazliitevaneizvorehraneza
pojedinevrsteriba.
Kljunerijei:slatkovodnaihtiofauna,Bliccabjoerkna,trofikapovezanost,liinkekukaca
Ichthyocenosesareoneofthekeyelementsinfunctioningofthelenticecosystems,insuringthe
necessary energy flow and nutrient recycling process within a water body. Research of the
composition and the structure of ichthyocenosis was conducted in July 2014, in Kopako Lake,
KopakiRitNaturePark.Totestthesignificanceofcertainfoodcategoriesinthefishdiet,their
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12.HBK23012 CBC
stomach contents were examined, given that fish can significantly influence benthos and
periphyton.Samplingwasdoneusinggillnetplacedinastationarymannerwithasoakingtimeof
12h,afterwhichitwasretrieved.Sampledfishwerefixedin4%formaldehydeforlaterlaboratory
measurements.Thecatchperuniteffort(CPUE)was2.71kgpernethours.Duringtheresearch,
143 fish specimens were sampled, aged from 1+ to 6+, belonging to 9 species of families
Esocidae, Cyprinidae and Percidae. The most abundant species was Blicca bjoerkna, with 50
specimens recorded. In general, more males than females were found during the research.
Different categories of food: vegetation, detritus, invertebrates, with the dominance of Insecta
larvae, and fish were found in the alimentary canals. Results indicate that sampled fish can be
classified as omnivores, benthivores and piscivores. We also recorded endoparasites in the
representatives of B. bjoerkna, Alburnus alburnus and Gymnocephalus baloni. Recorded
ichthyofauna is typical for this area and the type of habitat, whiles different feeding habits
indicateddifferentimportantfoodsourcesfortherecordedspecies.
Keywords:freshwaterichthyofauna,trophicrelationships,Insectalarvae
P94
RELATIONSHIPS BETWEEN ALGAL PRODUCTION AND THE PHYSICAL AND CHEMICAL
PROPERTIESOFADRINKINGWATERRESERVOIR
I.Grigorszky1,M.GligoraUdovi2,I.Somlyai1,B.Palombi1,L.Czvek1,Gy.Dvai1,S.A.Nagy1.
1
DepartmentofHydrobiology,FacultyofScience,UniversityofDebrecen,Debrecen,Hungary
(grigorszky.istvan@science.unideb.hu)
2
Division of Botany, Department of Biology, Faculty of Science, University od Zagreb, Zagreb,
Croatia
Keywords:reservoirs,algalbloom
P95
TAKSONOMSKI SASTAV MORSKIH BENTOSKIH DIJATOMEJA I NJEGOV ODNOS S GLAVNIM
EKOLOKIMIMBENICIMAUBOSNIIHERCEGOVINI
N.Jasprica1,D.Hafner2,A.Car1,T.Kapetanovi3
1
Laboratorij za ekologiju planktona i populacijsku genetiku, Institut za more i priobalje,
SveuiliteuDubrovniku,Dubrovnik,Hrvatska(nenad.jasprica@unidu.hr)
2
Bartulovii4,Blace,Hrvatska
3
FondzazatituokoliaFederacijeBosneiHercegovine,Sarajevo,BosnaiHercegovina
12.HBK23112 CBC
Kljunerijei:Neumskizaljev,bentos,dijatomeje,istoniJadran,SISredozemlje
N.Jasprica1,D.Hafner2,A.Car1,T.Kapetanovi3
1
Laboratory of Plankton Ecology and Population Genetics, Institute for Marine and Coastal
Research,UniversityofDubrovnik,Dubrovnik,Croatia(nenad.jasprica@unidu.hr)
2
Bartulovii4,Blace,Croatia
3
Environmental Fund of the Federation of Bosnia and Herzegovina, Sarajevo, Bosnia and
Herzegovina
IntheeasternAdriatic,thereareonlyafewstudiesonbenthicdiatomsmostlyfocusedon
ecology and taxonomy of periphytic diatoms in the Croatian estuaries or Albanian coastal
wetlands.Untilnow,bothplanktonicandbenthicmarinediatomshaveneverbeenstudied
in Bosnia and Herzegovina. This study forms part of a research program investigating the
biodiversity and ecosystem functioning in the border marine coastal waters between the
two countries Croatia and Bosnia and Herzegovina. The program includes phycological,
zoologicalandhydrographicalresearch.Thispaperconsidersthetaxonomiccompositionand
seasonality of marine benthic diatoms in the relation to some key environmental variables
(temperature, salinity and density) in the Bay of Neum in Bosnia and Herzegovina on the
southeasternAdriaticcoast.
Keywords:NeumBay,benthicdiatoms,AdriaticSea,SEMediterranean
P96
RASPROSTRANJENOSTIPROCJENAVELIINEPOPULACIJEVIDRE(LutralutraL.)UALPSKOJ
BIOGEOGRAFSKOJREGIJIHRVATSKE
M.Jeli1,T.arevi2,K.Jeli3,I.Katanovi4
1
Sveuilite u Zagrebu, Prirodoslovnomatematiki fakultet, Bioloki odsjek, Zoologijski
zavod,Zagreb,Hrvatska(misel.jelic@biol.pmf.hr)
2
Tratinska14,Zagreb,Hrvatska
3
EkolokaudrugaEmys,DonjiMiholjac,Hrvatska
4
UdrugaBIOM,Zagreb,Hrvatska
12.HBK23212 CBC
odreenekoordinatelokalitetaistraivanja.Terenskoistraivanjejeprovedenoulistopadui
studenom2013.godine.Kaoznakoviprisutnostividre,dokumentiranisuizmeti,elatinozne
izluevine, otisci stopala, ostaci hranjenja, staze, sklonita i brlozi. Ukupno je planirano
istraivanje na 100 lokaliteta, no dio lokaliteta (23) nije bilo mogue istraiti zbog njihove
nedostupnosti ili zbog opasnosti od mina. Utvrena je prisutnost vidre na 25 lokaliteta, s
ukupno 74 dokumentirana znaka njene prisutnosti. Ovim su istraivanjem potvreni stariji
podaci o nestanku vidre iz sljevova Gacke i Cetine, te gornjeg toka rijeke Kupe. Prisutnost
vidrejeponovnopotvrenauslijevurijekeLiketeulovinakograakimtekuicama,Otuii
Riici.Dobivenasunovasaznanjaoprisutnostivrsteumanjimkrkimvodotocimauokolici
Korenice,upotokuSuikkodmjestaDrenica,teurijeciButinici.Veliinapopulacijevidre
naistraenompodrujujeprocijenjenana50do70jedinki.
Kljunerijei:GorskiKotar,Lika,Dalmacija,standardnametoda
DISTRIBUTION AND ESTIMATED POPULATION SIZE OF THE OTTER (Lutra lutra L.) IN THE
ALPINEBIOGEOGRAPHICALREGIONOFCROATIA
M.Jeli1,T.arevi2,K.Jeli3,I.Katanovi4
1
UniversityofZagreb,FacultyofScience,DepartmentofBiology,DivisionofZoology,Zagreb,
Croatia(misel.jelic@biol.pmf.hr)
2
Tratinska14,Zagreb,Croatia
3
EcologicalSocietyEmys,DonjiMiholjac,Croatia
4
AssociationBIOM,Zagreb,Croatia
The data on distribution of the otter in the Alpine biogeographical region of Croatia were
outdatedorincomplete.Therefore,theaimofthisstudywastoinvestigatethepresenceof
otter in Gorski Kotar, Lika and a part of Dalmatia. The Standard method was used, as
recommended by the IUCN/SSC Otter Specialist Group. Before the field survey, potentially
suitable habitats for the otter were estimated, and thecoordinates of the survey locations
were determined using topographic maps and GIS applications. Field research was
conductedinOctoberandNovember2013.Thespraints,jellies,footprints,feedingremains,
paths, hovers and holts were documented as signs of the otter presence. In total, 100
locationswereplannedtobesurveyed,butalargepartofthem(23)werenotinvestigated
due to their inaccessibility or mine danger. The presence of otter was recorded at 25
localities, with a total of 74 signs of the otter presence. This research confirmed previous
dataonthespeciesdisappearancefromthebasinsofGackaandCetinarivers,andfromthe
upperKupa River. The presence of otter was again confirmed in the Lika, Otua and Riica
rivers.NewdataontheotterpresencewererecordedonsmallwatercoursesnearKorenica,
the Suik Stream near Drenica, and in the Butinica River. The estimated otter population
sizeisbetween50and70individuals.
Keywords:Otter,GorskiKotar,Lika,Dalmatia,Standardmethod
P97
BIOLOKOEKOLOKAOBILJEJAALUVIJALNIHPOTOKAMEIMURJA
D.KiNovak1,M.Kerovec2
1
Uiteljski fakultet Sveuilita u Zagrebu, Odsjek u akovcu, akovec, Hrvatska (darinka.kis
novak@ufzg.hr)
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12.HBK23312 CBC
DosadanjaistraivanjaaluvijalnihtekuicanapodrujuMeimurjasubilavrlonedostatna.Ova
istraivanjasuprvasustavnaistraivanjaitonapodvijepostajenapotocimaBoak(BO)iBistrec
(BI), a ukljuivala su hidroloka, fizikalnokemijska i faunistika obiljeja. Potok Bistrec je vei,
znatnobogatijivodom,prosjeangodinjiprotoknapostajiBI2iznosi2,3m3/sek,anapostajiBO2
tek 0,33 m3/sek. Znatne razlike postoje i u fizikalnokemijskim obiljejima. Osobito je znaajna
razlikauelektrovodljivostikojaupotokuBistrecuiznosi677777S/cm,tojeposljedicaznatno
veih vrijednosti Ca, Mg i sulfata te neto viih vrijednosti Ni, Pb i nitrita. U potoku Boaku
vrijednostielektrovodljivostisuznatnonie(288382S/cm)uznetovievrijednostipokazatelja
organskog oneienja: KPK, Cr, nitrata, ukupnog N i ortofosfata, vjerojatno kao posljedica
intenzivne poljoprivrede u slivu. Ukupno je utvrena prisutnost 66 svojti makrozoobentosa.
Znaajnije razlike raznolikosti zajednica izmeu istraivanih potoka nisu utvrene. Na svim
postajamaizrazitojenajbrojnijavrstaGammarusroeseli.JedinoodstupanjejenapostajiBI2na
kojoj je zabiljeena najmanja brojnost makrozoobentosa (1092 jed./m2) uz znaajni udio
predstavnika skupine Gastropoda, koji na ostalim postajama dolaze sporadino. Na ostalim
postajama brojnost makrozoobentosa je od 4100 do 5765 jed./m2. Prema Uredbi o standardu
kakvoevodatemeljemmakrozoobentosautvrenajedobraekolokakakvoa.
Kljunerijei:aluvijalnipotoci,biolokoekolokaobiljeja,makrozoobentos,Meimurje
BIOLOGICALECOLOGICALFEATURESOFALLUVIALSTREAMSOFTHEREGIONOFMEIMURJE
D.KiNovak1,M.Kerovec2
1
Faculty of Teacher Education in Zagreb, Extension in akovec, akovec, Croatia (darinka.kis
novak@ufzg.hr)
2
DepartmentofBiology,DivisionofZoology,FacultyofScience,UniversityofZagreb,Zagreb,Croatia
EarlierstudiesofalluvialstreamsintheRegionofMeimurjehadbeenveryinsufficientsothese
current studies presented the first systematic research. The studies included hydrological,
physicalchemicalandfaunisticcharacteristicsoftwostreams.TheBistrec(BI)islargerandmuch
richerinwaterthanBoak(BO).TheaverageannualflowatthestationBI2was2.3m/sec,and
at thestationBO2 itwasonly0.33 m/sec.Particularlysignificantisthedifferenceinelectrical
conductivity,whichinthestreamofBistrecwas677777S/cm,anditisaresultofmuchhigher
valuesofCa,MgandsulfateandaslightlyhighervaluesofNi,Pbandnitrite.Inthecaseofthe
creek of Boak, the values of electrical conductivity were significantly lower (288382 S/cm)
withslighltlyhighervaluesoftheindicatorsoforganicpollution:COD,Cr,nitrate,totalnitrogen
and orthophosphate, probably a consequence of intensive agriculture in the basin. The total
presenceof66speciesofmacrozoobenthoshavebeenfound.Atallstationsthemostnumerous
specieswasGammarusroeseli(Amphipoda).TheonlyexceptionwasthestationBI2wherethe
smallest number of macrozoobenthos have been registered (1092 Eq./m), with a significant
shareofrepresentativesoftheGastropodagroups,whichatotherstationscomesporadically.At
other stations the number of macrozoobenthos was in the range of 41005765 units/m.
AccordingtotheRegulationonwaterqualitystandards,itisgoodecologicalquality.
Keywords:alluvialstreams,biologicalecologicalfeatures,macrozoobenthos,Meimurje
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P98
VERTIKALNA RASPROSTRANJENOST, FAUNISTIKE I BIOGEOGRAFSKE ZNAAJKE DANJIH
LEPTIRA(INSECTA,LEPIDOPTERA)PARKAPRIRODEUKA
M.Kuini1,G.Mesari1,I.Mihoci2,E.Vasili3,M.Blaevi3,T.Koren4,T.Finderle1,Lj.Ladavac5,
M.Grgurev6
1
Bioloki odsjek, Zoologija, Prirodoslovnomatematiki fakultet Sveuilita u Zagrebu, Zagreb,
Hrvatska(mladen.kucinic@biol.pmf.hr)
2
Hrvatskiprirodoslovnimuzej,Zagreb,Hrvatska
3
ParkprirodeUka,Lovran,Hrvatska
4
HYLA,Hrvatskoherpetolokodrutvo,Zagreb,Hrvatska
5
VelaTraba5,Pazin,Hrvatska
6
GeoNatura,Zagreb,Hrvatska
Istraivanjadanjihleptira(Insecta,Lepidoptera)ParkaprirodeUkaobuhvaajuplaninskimasiv
Ukuidioiarijeaprovedenasuurazdobljuod2004.do2010.godine.Terenskoistraivanje
obuhvatilo je 23 lokalitet s razliitim ekolokim, geografskim, pedolokim, vegetacijskim i
hidrolokimznaajkama.Leptirisusakupljaniodtravnjadolistopada,prepariraniulaboratoriju
tedeterminiranistandardnimkljuevimazadanjeleptiredonivoavrste.Diovrstazabiljeenje
fotografiranjemnarazliitimlokalitetimailideterminiranjeprilikomopaajanaterenu.Ovim
istraivanjima utvreno je 90tak vrsta danjih leptira iz svih 5 europskih porodica. Meu
najzanimljivijimvrstamautvrenihtijekomovihistraivanjaistiuse:uskrnjileptir(Zerynthia
polyxena), crni apolon (Parnassius mnemosyne), kupusov bijelac (Pieris brassicae), kleopatra
(Gonepteryx cleopatra), movarni plavac (Maculinea alcon f. rebeli), ednjakov plavac
(Scolitantidesorion),trpuevaria(Melitaeacinxia),movarnaria(Euphydryasaurina),lani
sivac (Arenthusana arethusa), movarni debeloglavac (Heteropterus morpheus).
Biogeografskom analizom utvrena je dominacija eurosibirskih vrsta te znatno manje
mediteranskih vrsta.FaunadanjihleptiraUkeusporeenajesfaunomdanjihleptira: doline
gornjegtokarijekeKupe,Biokova,Velebita,DinareiPotaka.
Kljunerijei:Uka,danjileptiri,vertikalnarasprostranjenost,fauna
M.Kuini1,G.Mesari1,I.Mihoci2,E.Vasili3,M.Blaevi3,T.Koren4,T.Finderle1,Lj.Ladavac5,
M.Grgurev6
1
Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
(mladen.kucinic@biol.pmf.hr)
2
CroatianNaturalHistoryMuseum,Zagreb,Croatia
3
UkaNaturePark,Lovran,Croatia
4
HYLA,CroatianherpetologicaSociaty,Zagreb,Croatia
5
VelaTraba5,Pazin,Croatia
6
GeoNatura,Zagreb,Croatia
Investigationofbutterflies(Insecta,Lepidoptera)fromtheUkaNatureParkincludedhabitats
ontheUkaandiarijamountains.Fieldworkwasconductedbetween2004.and2010.,andit
included twentythree localities with different ecological, geographical, pedological and
hydrologicalcharacteristics.ButterflyspecimenswerecollectedfromApriltoOctoberperiod,
and determination was done in the laboratory according standard keys. Some species were
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12.HBK23512 CBC
established with photodata identification or determined directly in the field filed. In our
investigationweestablishedabout90butterflyspecies.Especiallyinterestingfindingsinclude:
SouthernFestoon(Zerynthiapolyxena),CloudedApollo(Parnassiusmnemosyne),LargeWhite
(Pierisbrassicae),Cleopatra(Gonepteryxcleopatra),AlconBlue(Maculineaalcon),Chequered
Blue (Scolitantides orion), Glanville Fritillary (Melitaea cinxia), Marsh Fritillary (Euphydryas
aurina), False Grayling (Arenthusana arethusa), Large Chequered Skipper (Heteropterus
morpheus). Biogeographical analyses of butterfly fauna in the Uka Nature Park showed
domination of the EuroAsian species, with smaller percentage of Mediterranean species. A
comparisonwasalsomadebetweenthefaunaestablishedintheUkaNaturePark,andupper
partoftheKupaRiver,Biokovo,Dinara,PotakandVelebitmountainsareas.
Keywords:Uka,butterflies,verticaldistribution,fauna
P99
SEZONSKE RAZLIKE U UNOSU, SKLADITENJU HRANE I TEINI DINARSKOG VOLUHARA
(Dinaromysbogdanovi)UZATOENITVU
M.Ljutina1,I.Katanovi2,M.Rezo3,I.Ivanek4,M.Damjanovi4,D.Lisii3
1
Dravnizavodzazatituprirode,Hrvatska(masa.ljustina@dzzp.hr)
2
UdrugaBIOM,Zagreb,Hrvatska
3
Zavodzaanimalnufiziologiju,Prirodoslovnomatematikifakultet,Zagreb,Hrvatska
4
ZoolokivrtgradaZagreba,Zagreb,Hrvatska
Zbogadekvatnogodravanjapopulacijeuzatoenitvu,kaoibuduihkonzervacijskihmjeraili
istraivanja biologije vrste, bilo je potrebno pribaviti podatke o prehrambenim potrebama
dinarskogvoluhara(Dinaromysbogdanovi).Stogasmoprovelijednogodinjeistraivanjeunosa
hrane te proizveli prvi popis prehrane ove vrste u zatoenitvu. Voluhari su prihvaali irok
rasponprehrane,uglavnomherbivorne,konzumiralisuuprosjeku41,27gsvjeemasepodanu
toiznosi6,64gsuhemase(0,54gsvjeemasei0,088gsuhemasepogramutjelesnemase)te
spremali u prosjeku 16,91 g svjee mase. Dnevna konzumacija svjee hrane po ivotinji
znaajno se razlikovala meu sezonama. ivotinje su konzumirale vie hrane tijekom ljeta i
jeseniuodnosunazimuiproljee.Takoer,postojalesuisezonskerazlikeuspremanjuhrane.
Tijekomproljeaspremanojeznaajnomanjehraneuodnosunazimu,tijekomljetaznaajno
manje u odnosu na zimu. ivotinje nisu imale konstantnu tjelesnu masu kroz godinu, bila je
znaajnomanjatijekomzimeiproljeauodnosunaljetoijesen.
Kljunerijei:boljojed,prehrana,konzervacijarijetkihvrsta,biologijavoluharica
SEASONAL DIFFERENCES IN FOOD INTAKE, CACHING AND BODY MASS OF THE ENDEMIC
BALKANSNOWVOLE(Dinaromysbogdanovi)INCAPTIVITY
M.Ljutina1,I.Katanovi2,M.Rezo3,I.Ivanek4,M.Damjanovi4,D.Lisii3
1
StateInstituteforNatureProtection,Zagreb,Croatia(masa.ljustina@dzzp.hr)
2
AssociationBIOM,Zagreb,Croatia
3
DepartmentofAnimalPhysiology,FacultyofScience,Zagreb,Croatia
4
ZoologicalGardenofZagreb,Zagreb,Croatia
Foradequatemaintenanceofcaptivepopulation,aswellasforfutureconservationeffortsor
studies of species biology, it was necessary to obtain diet demands for Balkan snow vole
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12.HBK23612 CBC
Keywords:herbivore,nutrition,rarespeciesconservation,volebiology
P100
FITOBENTOS I VODENI MAKROFITI KAO ELEMENTI BIOLOKE KVALITETE I KLASIFIKACIJE
STANITADUSUBPLANINSKEKRKERIJEKE
M.Mejdandi1,N.Maleevi1,2,N.Koleti1,2,M.Nemet1,K.KraljBorojevi1
1
Botaniki zavod, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska(maja.mejdandzic@biol.pmf.hr)
2
Institutzaistraivanjeirazvojodrivihekosustava,VelikaGorica,Hrvatska
Kljunerijei:fitobentos,vodenimakrofiti,Okvirnadirektivaovodama,Nacionalnaklasifikacija
stanita
M.Mejdandi1,N.Maleevi1,2,N.Koleti1,2,M.Nemet1,K.KraljBorojevi1
1
Division of Botany, Department of Biology, Faculty of Science, University od Zagreb, Zagreb,
Croatia(maja.mejdandzic@biol.pmf.hr)
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12.HBK23712 CBC
Instituteforresearchanddevelopmentofsustainableecosystems,VelikaGorica,Croatia
Key words: phytobenthos, aquatic flora, Water Framework Directive, National Habitat
Classification
P101
ZNAAKEIHTIOFAUNERIJEKESUTLE
M.Mrakovi1,M.aleta2,Z.Mari1,D.Zanella1,P.Mustafi1
1
Biolokiodsjek,Prirodoslovnomatematikifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
(milorad.mrakovcic@biol.pmf.hr)
2
Uiteljskifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
RijekaSutlaistraivanajestandardnommetodomelektroribolovanapetpostajanizvodnood
ulaska rijeke Sutle u Hrvatsku do ua u Savu. Ukupno je ulovljena 41 vrsta ribe. Porodica
Cyprinidae najbrojnija je vrstama i ukupnim brojem ulovljenih jedinki. Najbrojnija vrsta na
itavompodrujuistraivanjajedvoprugastauklija,abrojnoujeslijedebezribica,ploticai
klen. Od 33 zabiljeene vrste 29 je autohtono, a 4 vrste su alohtone. ShannonWienerov
indeksnasvimistraenimpostajamazajednoiznosi3,96.Strukturaukupnezajedniceopisana
je i modelom logaritamske serije s parametrima =5,3609; SE=0,8456 i x=0,9982. Od 33
zabiljeene vrste tijekom istraivanja pet ih je prisutno na svih pet postaja, tj. du gotovo
itavogtokarijeke,estjevrstaprisutnonaetiripostaje,sedamnatripostaje,estnadvije
postaje, a ak devet na samo jednoj postaji. Dominantna temperaturna grupa prema broju
vrsta u zajednici riba rijeke Sutle je toplovodna grupa koja ini 54% svih vrsta. Posebno su
zanimljivevrstekojesmatramorijetkimanapodrujuitaveHrvatskepoputKeslerovekrkue
(Romanogobio kessleri), tankorepe krkue (Romanogobio uranoscopus), malog vretenca
(Zingelstreber)idunavske paklare(Eudontomyzonvladykovi).Zbognjih, kaoizboggavice
(Rhodeus amarus), potone mrene (Barbus balcanicus) i pea (Cottus gobio), rijeka je Sutla
predloena kao jedno od znaajnih Natura 2000 podruja. Rijeka Sutla prema bioprodukciji
spadausrednjeproduktivneribolovnevode.
Kljunerijei:raznolikostriba,Natura2000
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12.HBK23812 CBC
FISHDIVERSITYOFTHERIVERSUTLA
M.Mrakovi1,M.aleta2,Z.Mari1,D.Zanella1,P.Mustafi1
1
FacultyofScience,UniversityofZagreb,Zagreb,Croatia
2
FacultyofTeacherEducation,UniversityofZagreb,Zagreb,Croatia
The river Sutla was investigated by the standard method of electrofishing on five stations,
fromtheentranceoftheriverinCroatiatothemouthoftheriver.Atotalof33speciesof
fishwererecorded.TheCyprinidaefamilywastherichestinspeciesaswellastotalnumber
ofindividualscaught.Themostabundantspeciesintheentireareaofinvestigationwasthe
Schneider and is followed by the Ston moroko, Danube roach and Chub. Of the 33 species
recorded,29areindigenousand4speciesareallochthonous.TheShannonWienerindexof
all investigated stations combined was 3.96. The structure of the total community is
describedbyalogarythmicseriesmodelwith=5.3609;SE=0.8456andx=0.9982.Outofthe
33species,fivewerepresentonallfivestations,sixwerepresentonfourstations,sevenon
three stations, six on two stations, and nine on only one station. Ecologically, the warm
watergroupisdominant(54%ofspecies).TheSutlariverhasspeciesthatweconsiderrare
throughout the entire area of Croatia, as the Kessler's gudgeon (Romanogobio kessleri),
Danube gudgeon (Romanogobio uranoscopus), Streber (Zingel streber) and the Danube
lamprey (Eudontomyzon vladykovi). Due to these species, as well as the European
bitterling(Rhodeusamarus)(Barbusbalcanicus)andBullhead(Cottusgobio),theriverSutla
has been selected as a Natura 2000 area. According to its bioproduction, the river Sutla
belongstothecategoryofmediumproductivefishingwaters.
Keywords:Fishcommunity,Fishecology,Natura2000
P102
POETNAISTRAIVANJAVASKULARNEFLOREISTANITAUZRIJEKUMURUUHRVATSKOJ
D.Prli
DonjiMeljani92C,Slatina,Hrvatska(prlicdragan@gmail.com)
Tijekomrazdobljaodtravnjadorujna2014.,provedenojebotanikoistraivanjedudonjeg
dijela rijeke Mure u Hrvatskoj. Istraivanje je zapoelo od sjeveroistone okolice naselja
Dekanovec,azavriloprijeuarijekeMureuDravublizuLegrada.Svaobraenapodrujau
ovom istraivanju nalazila su se izmeu rijeke Mure i nasipa koji slui obrani od poplavnih
voda. Ukupno je odabrano 19 interesnih podruja kojima se nastojalo dobiti uvid u biljnu i
staninu raznolikost. Obuhvaene su mrtvice i movare, zatim rukavci, jezera i dodirne
umske povrine, a posjeivani su u nekoliko navrata tijekom vegetacijske sezone.
Istraivanjem su zabiljeene ukupno 94 biljne svojte te niz vrijednih staninih tipova koji
pratenacionalnuklasifikacijustanita(NKS).Analizarezultatapokazujedaje23biljnihvrsta
zatienozakonom(15zatienih,8strogozatienih),a10vrstaposjedujestanovitistatus
Crvene liste (1 ugroena, 4 osjetljive, 3 gotovo ugroene, 2 nedovoljno poznate). Valja
naglasiti kako su odreena podruja posebno bogata biljnim vrstama, primjerice mrtvice
Fuika i Gombakut. Vrste kao to su Hottonia palustris, Stratiotes aloides ili Eleocharis
quinqueflora iznimno su rijetke. Istraivanje preostalog gornjeg dijela uz rijeku Muru
trenutnojeuprovedbi.
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12.HBK23912 CBC
Kljunerijei:Hrvatska,movarnopodruje,rijekaMura,stanita,vaskularnaflora
INITIAL RESEARCH OF VASCULAR FLORA AND HABITATS ALONG THE MURA RIVER IN
CROATIA
D.Prli
DonjiMeljani92C,Slatina,Croatia(prlicdragan@gmail.com)
During the period AprilSeptember of 2014, botanical research was conducted along the
lower part of the Mura River in Croatia. The survey started in the northeast vicinity of the
village Dekanovec and concluded just before the MuraDrava confluence near Legrad. All
surveyedareasinthisresearchwerelocatedbetweentheMuraRiverandtheembankment
that prevents flooding of the adjoining countryside. In order to gain insight into plant and
habitat diversity, 19 areas of interest were selected. These consisted of oxbow lakes and
marshes,followedbyriverforks,pondsandneighbouringwoodland,whichwerevisitedon
multipleoccasionsduringtheseason.Inthisresearch,atotalof94planttaxawererecorded
alongwithavarietyofvaluablehabitattypesaccordingtothenationalhabitatclassification
(NKS).Theanalysis oftheresultsshowsthat23plantspeciesareprotectedbythe law(15
protected,8strictlyprotected)and10specieshaveacertainRedListstatus(1endangered,
4vulnerable,3nearthreatened,2datadeficient).Itisworthnotingthatcertainareas,such
astheoxbowlakesFuikaandGombakut,werequiterichinplantspecies.Speciessuchas
Hottonia palustris, Stratiotes aloides or Eleocharis quinqueflora are particularly rare. The
researchoftheremainingupperpartalongtheMuraRiveriscurrentlyinprogress.
Keywords:Croatia,MuraRiver,habitats,vascularflora,wetlands
P103
STANITA SLATKOVODNIH MEDUZA (Craspedacusta sowerbyi LANKESTER 1880.) NA
PODRUJUDONJEGMEIMURJA
M.Raan,L.Raan,V.PircMezga
SrednjakolaPrelog,Prelog,Hrvatska(rasanmiso@gmail.com)
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12.HBK24012 CBC
Kljunerijei:slatkovodnameduza,mrtvice,ljunare,predatori
M.Raan,L.Raan,V.PircMezga
HighschoolPrelog,Prelog,Hrvatska(rasanmiso@gmail.com)
The topic of the research was the presence of freshwater jellyfish (Craspedacusta sowerbyi
Lankester1880)inthewatersoflowerMeimurje.Thetypeofbottomsediment,ichtyofauna
andwater'sphysicalproperties(pH,electricconductivityandtemperature)weredeterminedin
Perutnica (Drava's dead end near Otok) and gravel pit near Kotoriba. Units of jellyfishlike
animalshavebeengatheredatthementionedhabitatsatthesurfacelayerandatthedepthod
1.2meters.Fielddatagatheringwasdonefrom12thJulyto17thOctober2014.Alivematerial
wasgatheredeighttimesateveryresearchlocation(every2weeks).Collectedsampleswere
testedforpredators,thevariabilityofthediameterofjellyfishlikeanimalswasdetermined,as
wellasthenumberofunitsoffreshwaterjellyfishinacertainwatervolumeeverytwoweeks.
1014jellyfishlikeanimalsweregatheredatbothlocations,923inPerutnicaandonly98near
Kotoriba.Alargernumberofunitsatbothlocationsatthetimeofresearchwasatthedepthof
1.2 meters, and as the light intesity decreases, animals move up towards the surface.
Variabilityofthebody'sdiameterisincreasedduringresearchduetogrowthanditvariesfrom
0.8 to 2.5 centimeters. The research showed that the only possible tested predator of
freshwaterjellyfishisfreshwatercrayfish.
Keywords:freshwaterjellyfish,gravel,backwaters,predator
P104
QUALITATIVE ANALYSIS OF REPRESENTATIVES FROM ANNELIDA (OLIGOCHAETA AND
HIRUDINEA)FROMKUMANOVOBATH
S.Smiljkov1,D.Gusheski1,V.SlavevskaStamenkovikj1,R.Ilieska1,K.Krstevska1,S.Stojanovski2
1
Institute of Biology, Faculty of Natural Science and Mathematics, Skopje, Republic of
Macedonia2HydrobiologicalInstitute,Ohrid,Macedonia
From our research in the area of Kumanovo Bath, after conducting qualitative analysis of
samples collected in 4 locations of Kumanovo Bath, we determinated 11 species of the type
AnnelidathatbelongtotheclassesOligochaetaandHirudinea.Basedonourresults,5species
belong to the class of Oligochaeta and 6 to the class of Hirudinea. From the class of
Oligochaeta, following species were determinated: Stylaria lacustris, Tubifex tubifex,
Limnodrilushoffmeisteri,L.udekemianusthatbelongtothefamilyTubificidaeandHaplotaxis
gordioides that belongs to the family Haplotaxidae. From the class of Hirudinea we
determinated following species: Piscicola geometra that belongs to the family Piscicolidae,
Glossiphonia complanata from the family Glossiphonidae, Haemopis sangusuga from the
family Haemopidae, Hirudo medicinalis from the family Hirudidae, Erpobdella octoculata and
Dina sp. from the family Erpobdeliidae. Dominant species from the class of Oligochaeta are
LimnodrilushoffmeisteriandHaplotaxisgordioides,andfromtheclassofHirudineaareHirudo
medicinalisandErpobdellaoctoculata.
Keywords:Kumanovobath,classOligochaeta,classHirudinea,species
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12.HBK24112 CBC
P105
VERTIKALNA DISTRIBUCIJA MORFOLOKIH OBLIKA VRSTE Cylindrospermopsis raciborskii U
POPLAVNOMJEZERU
F.Stevi1,A.Mili2,D.poljariMaroni1,T.unaPfeiffer1,V.Zahirovi1,M.Mihaljevi1
1
Zavod za ekologiju voda, Odjel za biologiju, Sveuilite Josipa Jurja Strossmayera u Osijeku,
Osijek,Hrvatska(fstevic@biologija.unios.hr)
2
ONikoleTesle,Vinkovci,Hrvatska
Kljunerijei:invazivnevrste,primarniisekundarnifilamenti,trihomi,akinete,heterocite
VERTICALDISTRIBUTIONOFMORPHOLOGICALFORMSOFCylindrospermopsisraciborskiiINA
FLOODPLAINLAKE
F.Stevi1,A.Mili2,D.poljariMaroni1,T.unaPfeiffer1,V.Zahirovi1,M.Mihaljevi1
1
SubdepartmentofWaterEcology,DepartmentofBiology,JosipJurajStrossmayerUniversity
ofOsijek,Osijek,Croatia(fstevic@biologija.unios.hr)
2
ONikoleTesle,Vinkovci,Croatia
ThecyanobacteriumCylindrospermopsisraciborskii(Woloszynska)SeenayaetSubbaRajuisa
toxic,invasive,originallytropicaltosubtropicalspecies,whichhasspreadinmanyfreshwater
ecosystems of temperate regions. The aim of this study was to determine the vertical
distribution and morphological changes of C. raciborskii in summer of 2011 in Lake Sakada
(KopakiritNaturePark).Sampleswerecollectedatthesurfaceandateverymeterdepthto
the lake bottom. At the beginning of the development (in August), the presence of only one
morphological form (primary filament) of C. raciborskii was recorded (1.21 x 106 ind./L). In
September,themassivedevelopmentofthisspecieswasfound(27.81x106ind./L).Together
with the primary filament, there were six other morphological forms of secondary filament.
Themorphologicalformsdifferentiatedaccordingtothesize(lengthandwidth)offilamentas
well as akinete size. Trichomes with one heterocyte were the most represented secondary
filaments in the whole water column. Their presence was between 59.01% (in the bottom
layer) to 84.85% (in the surface layer) of the total number of C. raciborskii individuals.
Continuousmonitoringofthisinvasivespecieswillcontributetospreadcontrolandpromote
water protection measures and biodiversity of Kopaki Rit floodplain, as well as other
freshwaterecosystemsofcentralandsouthernEurope.
Keywords:invasivespecies,primaryandsecondaryfilaments,trichomes,akinetes,heterocytes
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12.HBK24212 CBC
P106
ANTROPOGENI UTJECAJ NA RAZNOLIKOST BENTIKIH BESKRALJENJAKA SREDNJEG TOKA
RIJEKEDRAVEIPRIPADAJUIHLJUNARA
I.Strbad1,V.MietiStankovi2,3,M.Raan4,M.Kuini3
1
I.Osnovnakolaakovec,akovec,Hrvatska(ivanastrbad123@gmail.com)
2
Hrvatskiprirodoslovnimuzej,Zagreb,Hrvatska
3
Biolokiodsjek,Zoologijskizavod,Prirodoslovnomatematikifakultet,Zagreb,Hrvatska
4
SrednjakolaPrelog,Prelog,Hrvatska
Makrozoobentos ine ivotinje koje ive na dnu slatkovodnih stanita koji se esto koriste
kao bioindikatori. Tako su primarni ciljevi istraivanja bili utvrditi sastav ivotinjskog svijeta
dna u razliitim tokovima rijeke Drave i pripadajuih ljunara. Nadalje, utvrditi i utjecaj
temperature vode i otopljenog kisika zajedno s antropogenim utjecajem na zajednicu
makrozoobentosa. Istraivanje je provedeno na osam postaja na rijeci Dravi i pet
pripadajuihljunara.UzorkovanjejesvakadvamjesecaobavljanonarijeciDraviodtravnja
do prosinca 2012., a na ljunarama od srpnja 2013. do oujka 2014. godine. Analizom
odnosa zajednice makrozoobentosa i fizikalnokemijskih pokazatelja vode utvrena je
znaajna korelacija skupina Hirudinea, Copepoda i Coleoptera s temperaturom vode i
koliinom otopljenog kisika. Takoer je utvrena vea brojnost indikatorskih skupina istih
voda Turbellaria, Hirudinea i Plecoptera na postajama gdje je bio najmanji antropogeni
utjecaj. Na postajama gdje je kanalizirana rijeka Drava ili okruena poljoprivrednim
zemljitima dominiraju skupine Copepoda i Diptera. Kod ljunara koje su pod jakim
antropogenim utjecajem prikupljene su skupine koje podnose vea organska oneienja
poputtrzalacaivodenihgrinja.Sdrugesustraneuljunaramabezizraenogantropogenog
utjecaja dominirali vodencvjetovi. Zakljuno, makrozoobentos rijeke Drave, kao i okolnih
ljunara je pokazao visok stupanj bioraznolikosti, ali i osjetljivosti na razliite antropogene
utjecaje,toukazujenavanostouvanjaovogpodruja.
Kljunerijei:rijekaDrava,ljunare,makrozoobentos,bioraznolikost,osjetljivost
I.Strbad1,V.MietiStankovi2,3,M.Raan4,M.Kuini3
1
I.Elementaryschoolakovec,akovec,Croatia(ivanastrbad123@gmail.com)
2
CroatianNaturalHistoryMuseum,Zagreb,Croatia
3
DepartmentofBiology,DivisionofZoology,FacultyofScience,Zagreb,Croatia
4
HighschoolPrelog,Prelog,Croatia
Asbenthicinvertebratesinhabitalltypesoffreshwaterhabitatsoftenareusedasbioindicators,
especiallyinareasofaspecialinterestlikeDravaRiver.Therefore,theprimaryobjectiveofthis
studywastodeterminethecompositionofmacrozoobenthosindifferentsitesofDravaRiverand
the accompanying gravel pits. Secondly, to determine the impact of water temperature and
dissolved oxygen together with anthropogenic pressure on studied communities. The research
wasconductedateightstationsontheDravaRiverandatfivegravelpits.Samplingintheriver
wasperformedeverytwomonthsfromApriltoDecember2012,andatthegravelfromJuly2013
to March 2014. Analysis of the relationship of macroinvertebrates and physicochemical
parametersofwaterresultedinsignificantcorrelationforHirudinea,CopepodaandColeoptera.
Also, a higher number of clean water indicators, Turbellaria, Hirudinea or Plecoptera, were
identifiedatstationswhereanthropogenicpressurewastheleast.AtstationswhereDravaRiver
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12.HBK24312 CBC
is canalised or agricultural lands occur Copepoda and Diptera dominated. In gravel that were
under strong anthropogenic influence dominated Chironomidae and Hydrachnaellae, unlike in
gravel with natural conditions where Ephemeroptera was a dominant group. In conclusion,
macrozoobenthosofDravaRiverandsurroundinggravelhasshownahighlevelofbiodiversity,
but also the sensitivity on different anthropogenic impacts, which indicates the importance of
preservingthisarea.
Keywords:Dravariver,gravelpits,macrozoobenthos,biodiversity,sensitivity
P107
DINAMIKA NASELJAVANJA I SUKCESIJE TREPETLJIKAA U PERIFITONU JEZERA POPLAVNOG
PODRUJA
B.Vlaievi,J.Vidakovi
Zavod za ekologiju voda, Odjel za biologiju, Sveuilite Josipa Jurja Strossmayera u Osijeku,
Osijek,Hrvatska(bleskovic@biologija.unios.hr)
Istraivanjepromjenaustrukturizajednicetrepetljikaauperifitonurazvijenomnaumjetnim
podlogama (predmetna stakalca) provedeno je u poplavnom podruju Dunava (Sakadako
jezero,ParkprirodeKopakirit).Istraivanjejeprovedenourazdobljuodtravnjadoprosinca
2009. godine. U svrhu praenja procesa naseljavanja, uzorci su na poetku istraivanja
prikupljeni nakon 1., 3., 5., 7. i 14. dana ekspozicije, nakon ega je uzorkovanje provedeno
svakadvatjedna,kakobisepratilasukcesijatrepetljikaauzajedniciperifitona.Zabiljeeno
jeukupno116svojtitrepetljikaa.Ustanovljenesutrifazeurazvojuzajednice:inicijalna(1.
5. dan), intermedijarna (7. 14. dan) te zrela faza (28. 224. dan). U inicijalnoj i
intermedijarnojfazinajznaajnijiutjecajnazajednicuimalisuprozirnostitemperaturavode,
priemusukarakteristinibilipokretnibakteriovorniialgivornitrepetljikai.Organskatvaru
perifitonu, fitoplankton i konduktivitet najvie su utjecali na zajednicu u zreloj fazi razvoja,
kadasubilezastupljenerazliitetrofikeskupinetrepetljikaa,posebnosjedilakiorganizmi
koji se hrane filtriranjem, te predatori. Na poetku naseljavanja organizama na podlogu,
abiotiki parametri esto imaju izraeniji utjecaj, dok su biotiki parametri znaajniji u
oblikovanjuveformiranezajedniceperifitona.
Kljunerijei:trepetljikai,razvojperifitona,umjetnepodloge,trofikastruktura
B.Vlaievi,J.Vidakovi
SubdepartmentofWaterEcology,DepartmentofBiology,JosipJurajStrossmayerUniversityof
Osijek,Osijek,Croatia(bleskovic@biologija.unios.hr)
12.HBK24412 CBC
taxawasrecordedinperiphytonduringtheinvestigation.Threephasesinthedevelopment
ofperiphyticciliatecommunitywereobserved:initial(days1to5),intermediate(days7to
14)andmature(days28to224).Watertransparencyandwatertemperaturewerethemost
important parameters influencing the initial and intermed iate phases of community
development when mainly vagile bacterivorous and algivorous ciliates were present. The
mature phase was most affected by periphyton organic matter, phytoplankton and
conductivity,indicatingfoodsourceasprimaryfactorinfluencingperiphyticciliates.Ciliates
with broader food spectrum characterized the community, especially sessile filter feeders
andpredators.Itisoftenthecasethatabioticparametersinfluencetheinitialcolonization
process,whilesbioticparameterscanbemoreimportantforthestructuringoftheformed
periphyticcommunity.
Keywords:ciliatedProtozoa,periphytondevelopment,artificialsubstrata,trophicstructure
P108
MIKROBNIBIOFILMPERIODIKOGSLATKOVODNOGKRKOGIZVORA
P.utini1,I.Petri2,M.GligoraUdovi1,K.KraljBorojevi1,I.Mikac2,I.Bokan3,I.poljari4,G.
Mri4,J.Kamberovi5,A.PlenkoviMoraj1,S.Gottstein1,I.Ternjej1
1
Prirodoslovnomatematikifakultet,Biolokiodsjek,Zagreb,Hrvatska(petar.zutinic@biol.pmf.hr)
2
InstitutRuerBokovi,Zavodzaistraivanjemoraiokolia,Zagreb,Hrvatska
3
NastavnizavodzajavozdravstvoPrimorskogoranskeupnije,Rijeka,Hrvatska
4
Centarzaforenzinaispitivanja,istraivanjaivjetaenja"IvanVueti",Zagreb,Hrvatska
5
SveuiliteuTuzli,Prirodnomatematikifakultet,Biolokiodsjek,Tuzla,BosnaiHercegovina
Tijekom zime 2013. i proljea 2014. godine sa izvora Kri uzorkovani su uzorci mikrobne
zajednice te fizikokemijski imbenici kako bi se odredila promjena u sastavu zajednice
izvora. Dijatomeje su brojane i taksonomski odreene pomou pretranog elektronskog
mikroskopa (SEM). Nakon izolacije DNA iz cijanobakterija, koritena je PCR (polimerazna
lananareakcija)metodazaamplifikacijucijanobakterijskih16SrRNAfragmenatakakobise
dobile iste klonske sekvence za svaki pojedini uzorak. Usporedbom dobivenih sekvenci sa
bazom podataka (GenBank) izraena su filogenetska stabla slinosti, pri emu su sekvence
pokazale visoki stupanj homologije (9899%) sa 16S rRNA genima skupine Oscillatoriales
(rodovi Tychonema, Pseudoanabaena, Hydrocoleum, Wilmottia te vrsta Phormidium
autumnale). Rezultati meusobne usporedbe sekvenci pokazali su izrazito filogenetsko
razdvajanje u 2 grupe: (i) "Phormidium", te (ii) "Wilmottia", sa 710% razlike u sastavu 16S
rRNA. Uz to, grupa "Wilmottia", koju ini iskljuivo zimski uzorak, pokazala je 99%
homolognost/srodnostsWilmottiasp.CAWBG522izolatomsNovogZelanda,topredstavlja
prvi nalaz tog cijanobakterijskog ribosomalnog tipa u Hrvatskoj. Takoer je, unato vrlo
visokom stupnju homologije, dolo do dodatnog razdvajanja unutar samih grupa
"Phormidium" i "Wilmottia" u podgrupe s proljetnim i zimskim klonovima, to upuuje na
mogunostdacijanobakterijeizKriapredstavljajugenotipovespecifinezahrvatskuregiju.
Kljunerijei:mikrobnezajednice,krkiizvor,Phormidium
MICROBIALMATSININTERMITTENTFRESHWATERKARSTICSPRING
P.utini1,I.Petri2,M.GligoraUdovi1,K.KraljBorojevi1,I.Mikac2,I.Bokan3,I.poljari4,G.
Mri4,J.Kamberovi5,A.PlenkoviMoraj1,S.Gottstein1,I.Ternjej1
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12.HBK24512 CBC
FacultyofScience,DepartmentofBiology,Zagreb,Croatia(petar.zutinic@biol.pmf.hr)
RuerBokoviInstitute,DivisionforMarineandEnvironmentalResearch,Zagreb,Croatia
3
TeachingInstituteofPublicHealthofPrimorskogoranskaCounty,Rijeka,Croatia
4
ForensicScienceCentre"IvanVueti",Zagreb,Croatia
5
UniversityofTuzla,FacultyofScience,DepartmentofBiology,Tuzla,BosniaandHerzegovina
Livesamplesofmicrobialmatcommunity,togetherwithmainecologicalparameters,were
taken during winter 2013 and spring 2014 in Kri Spring. Diatoms were counted and
identifiedusingscanningelectronmicroscopy(SEM).AfterDNAisolationofcyanobacterial
species,PCR(polymerasechainreaction)wasusedfortheamplificationofcyanobacterial
16S rRNA fragments to obtain pure clonal sequences for each sample. The resulting
sequences were compared to the database (GenBank) and based on the similarity
phylogenetictreeswereconstructed.Thesequencesshowedhighhomology(9899%)with
the 16S rRNA gene of the Oscillatoriales (genera Tychonema, Pseudoanabaena,
Hydrocoleum,WilmottiaandPhormidiumautumnale).Sequencesgroupedinto2separate
clusters: (i) "Phormidium", and (ii) "Wilmottia". Sequence comparison showed that there
was710%differencein16SrRNAamongthetwoclusterspresented.Theresultsindicate
a distinct phylogenetic separation between the "Phormidium" and "Wilmottia" clusters.
Furthermore, "Wilmottia" cluster originated exclusively from the sample collected in
winterandshowed99%homologytoWilmottiasp.CAWBG522isolatefromNewZealand.
Despite veryhighhomology,sequences formeddistinct subclustersinside "Phormidium"
and "Wilmottia" cluster with spring clones being further separated from winter ones,
which indicates that cyanobacteria from the karstic Kri Spring represent genotypes
specificfortheCroatianregion.
Keywords:microbialmat,karsticspring,Phormidium
TOKSIKOLOGIJAIEKOTOKSIKOLOGIJA
TOXICOLOGYANDECOTOXICOLOGY
2
P109
PROCJENAUTJECAJAOTPADNOGMULJANAOKOLIMOGUIBIOMARKERI
12.HBK24612 CBC
biomarkeriukazalisunaznaajnepromjenenastaninojraziniitkivnojrazini,potvrujuikako
otpadnimuljmoepredstavljativelikirizikzaorganizmekojiiveutlu.
Kljunerijei:toksikologijatla,gujavica,mehanizammultiksenobiotikeotpornosti,histologija,
lipidnaperoksidacija
POTENTIALBIOMARKERSFORSEWAGESLUDGEPOLLUTIONMONITORING
S.Babi1,R.SauerbornKlobuar1,J.Barii1,G.Klobuar2,N.TopiPopovi1,I.StrunjakPerovi1,R.
oRakovac1
1
LaboratoryforIchtiopathologybiologicalmaterials,DivisionofMaterialsChemistry,Rudjer
BoskovicInstitute,Zagreb,Croatia
2
Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
(goran.klobucar@biol.pmf.hr)
The increased production of sewage sludge from municipal waste water treatment plants
(WWTP) requires alternative methods for its final disposal. Since sewage sludge contains
nutrientsthatenhancesoilproperties,landapplicationisusuallythemosteconomicdisposal
alternative. However, sewage sludge may contain toxic substances (heavy metals,
pharmaceuticalsandotherxenobiotics)thatmayposeapotentialthreattoterrestrialfauna,
andconsequentlytohumans.Toxicpotentialofliquidactivesludgeandsemisolddepotsludge
(active sludge redeposited on a nearby land location) was evaluated by earthworm
histopathological analysis, changes in the multixenobiotic resistance activity and lipid
peroxidation analysis after acute, semiacute and subchronic exposure period. Applied
biomarkers demonstrated extensive alterations at the cellular and tissue levels, proving that
sewagesludgecanposeaseriousriskforsoilorganisms.
P110
PROCJENA TOKSINOSTI KOMUNALNIH OTPADNIH VODA IZ POSTROJENJA ZA
PROIAVANJE OTPADNIH VODA KORITENJEM TESTA EMBRIOTOKSINOSTI NA
ZEBRICAMA
12.HBK24712 CBC
Kljunerijei:embrijzebrice,otpadnevode,toksinost,teratogenost
In recent years zebrafish (Danio rerio) embryo became frequently used model organism in
ecotoxicologyresearch.Duetotheirshortembryonicdevelopmentandtransparentchorion
zebrafishembryoshavebeenshowntobeausefultoolforthedetectionoftoxicsubstances
andobtainedresultscorrelatewellwithresultsobtainedformammals.Forthatreason,toxic
potential of waste waters from wastewater treatment plant Virovitica that receives
municipal, hospital and sugar plant wastewaters was evaluated on zebrafish embryos. By
conducting fish embryo toxicity test (FET) we summarized embryotoxic/teratogenic effects
and gained insight into the toxicity of pretreated and treated waste waters on aquatic
organisms.Obtainedresultsshowthatzebrafishembryocanbeusedasavaluabletoolfor
potentialtoxicevaluationofwastewaters.
Keywords:zebrafishembryo,wastewater,toxicity,teratogenicpotential
P111
CAN HISTOPATHOLOGICAL INVESTIGATION BE A MARKER OF POLLUTION IMPACTS? A CASE
STUDYOFSOMEECONOMICALLYIMPORTANTFISHSPECIESINTHEEUTHROPHICLAKEMOGAN,
CENTRALANATOLIA
G.Gl1,A..KarasuBenli2,F.Erko3,A.Gl3
1
Health Services Vocational School, Environmental Health and Environmental Sciences
Program,GaziUniversity,Ankara,Turkey(goktuggul@gazi.edu.tr)
2
Graduate School of Natural and Applied Sciences, Environmental Sciences Department, Gazi
University,Ankara,Turkey
3
BiologyEducationDepartment,GaziFacultyofEducation,GaziUniversity,Ankara,Turkey
ThisstudywascarriedoutinMoganLake,duringthebreedingperiodoffishspeciesbetween
JanuaryandMay2013.MoganLakeisanimportantrecreationalandfishingfreshwatersource,
located in South of Ankara. The water quality parameters of: temperature, pH, dissolved
oxygen, conductivity, total dissolved solids, salinity, alkalinity, bromine, copper, zinc, iron,
floride, phosphate, calcium, chloride, chromium, magnesium, manganese, nitrite, nitrate,
nickel, potassium, sulphate and total hardness were analyzed in Mogan Lake samples.
EconomicfishspeciesofCyprinuscarpio,Carassiuscarassius,TincatincaandEsoxluciuswere
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captured simultaneously. Gonads, gill, muscle, kidney, liver and peritonium tissues were
examinedhistologically,andcomparedwithacontrolgroupfromanuncontaminatedsampling
area.Histologicalexaminationresults:branchitis,hyperemia,epitelialliftingandfusiongillthe
secondary lamellae, hydropic degeneration and passive hyperemia in liver, hemorrhage in
kidneys,oophoritisandseperationofthebasalmembraneonovariumtissues,degenerationof
testis tissue, peritonitis on peritonium of the intestine. The histopathology results showed
them to be good biomarkers for studying and monitoring anthropogenic pollution/factors
impactsoninlandwatersystems.
Keywords:MoganLake,ecotoxicology,histopathology,waterquality
P112
PROCJENA TOKSINOSTI VODENOG EKSTRAKTA LISTA OBINE PLANIKE (Arbutus unedo L.) I
ARBUTINANALJUDSKIMLIMFOCITIMAPERIFERNEKRVIUUVJETIMAINVITRO
K.Jurica1,S.egan2,D.MilojkoviOpsenica3,I.BriKaraonji4,V.Benkovi5,N.Kopjar4
1
MinistarstvounutarnjihposlovaRH,Zagreb,Hrvatska(karlo.jurica@gmail.com)
2
Institut za hemiju, tehnologiju i metalurgiju Centar za hemiju, Univerzitet u Beogradu,
Beograd,Srbija
3
Hemijskifakultet,UniverzitetuBeogradu,Beograd,Srbija
4
Institutzamedicinskaistraivanjaimedicinurada,Zagreb,Hrvatska
5
Prirodoslovnomatematikifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
Obinaplanika(ArbutusunedoL.)samoniklajevazdazelenabiljkakojasetradicionalnokoristiu
pukojmedicini.Mehanizmidjelovanjanjenihsastavnica,osobitonastaninojrazini,jouvijek
nisu u potpunosti razjanjeni. Meu brojnim fenolnim spojevima prisutnim u listu planike po
ljekovitostiseizdvajaarbutin.Stogajeciljnaihistraivanjabioizmjeritisadrajtogfenolnog
glikozida u ispitivanom ekstraktu lista planike te utvrditi koje bioloke uinake ekstrakt lista
planike i arbutin mogu izazvati u ljudskim stanicama. Biljni materijal prikupljen je na Malom
Loinju (5/2013.). Iz osuenih listova ekstrahirani su fenolni spojevi te je provedena
kvantitativna analiza arbutina primjenom tekuinske kromatografije visoke djelotvornosti uz
detekcijuUVdetektoromsnizomdioda(HPLCDAD).Ekstraktjesadravao10,7mgarbutinapo
gramuliofilizata.Uinvitropokusunalimfocitimaperifernekrviistrailismouinkearbutinai
ekstrakta lista, primijenjenih u tri koncentracije: 11,4 mg/kg (odgovara najveem dnevno
prihvatljivomunosuekstraktauorganizam),200mg/kg(koncentracijaiznadkojesuuranijim
istraivanjima uoeni toksini uinci) i 400 mg/kg. Nakon 24satnog izlaganja limfocita
procijenjen je citotoksini potencijal testiranih uzoraka (primjenom fluorescencijskog testa
preivljenja); potencijal za izazivanje primarnih oteenja DNA (primjenom komettesta),
odnosno citogenetikih oteenja (primjenom mikronukleustesta). Ekstrakt lista planike i
arbutin imaju niski citotoksini i genotoksini potencijal te ne izazivaju znaajan porast
uestalosti mikronukleusa u limfocitima. Pri istim koncentracijama, ekstrakt lista planike
pokazujeveucitotoksinostodarbutina.Meutim,arbutin,zarazlikuodekstrakta,zamjetnije
usporava stanini ciklus limfocita. Uoeni antiproliferacijski uinak vrijedan je daljnjih
istraivanjanadrugimvrstamastanica,aosobitotumorskimlinijama.
Kljunerijei:citotoksinost,HPLC,limfociti,ljekovitobilje,primarnaoteenjaDNA
TOXICITYASSESSMENTOFSTRAWBERRYTREE(ArbutusunedoL.)WATERLEAFEXTRACTAND
ARBUTININHUMANPERIPHERALBLOODLYMPHOCYTESINVITRO
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K.Jurica1,S.egan2,D.MilojkoviOpsenica3,I.BriKaraonji4,V.Benkovi5,N.Kopjar4
1
TheRepublicofCroatiaMinistryoftheInterior,Zagreb,Croatia(karlo.jurica@gmail.com)
2
Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, University of
Belgrade,Belgrade,Serbia
3
FacultyofChemistry,UniversityofBelgrade,Belgrade,Serbia
4
InstituteforMedicalResearchandOccupationalHealth,Zagreb,Croatia
5
FacultyofScience,UniversityofZagreb,Zagreb,Croatia
Strawberrytree(ArbutusunedoL.)isanevergreentreecommonlyusedintraditionalmedicine.
Themechanismsthatunderliethebiologicaleffectsofitscomponents,especiallyatthecellular
level, have not yet been fully explained. Since arbutin constitutes the most important
component of the plants extract, our research focused mostly on this glycoside. This study
aimed to determine arbutin content in strawberry tree water leaf extract, and evaluate the
biological effects caused by the extract and arbutin in human cells. The plant material was
collected on the island of Mali Loinj, Croatia (5/2013). Phenolic compounds were extracted
from dried leaves and extracts were subjected to quantitative analysis of arbutin using high
performance liquid chromatography with UV diode array (HPLCDAD) detection. The extract
contained10.7mgarbutinpergoflyophilisate.Usinghumanperipheralbloodlymphocytesin
vitro,westudiedtheeffectsofarbutinandstrawberrytreewaterleafextractappliedatthree
concentrations: 11.4 mg/kg (corresponding to the maximum acceptable daily intake of the
extract),200mg/kg(previousstudiesreportedtoxiceffectsabovethisconcentration)and400
mg/kg. After a 24hour exposure, we evaluated the cytotoxic effects of the tested samples
(usinganacridineorange/ethidiumbromide doublestainingviability assay);the potentialfor
induction of primary DNA damage (using the alkaline comet assay), and cytogenetic damage
(using the micronucleus assay) in treated and control lymphocytes. Both the strawberry tree
leafextractandarbutinhadlowcytotoxicandgenotoxicpotential.Theyalsodidnotcausea
significant increase in the frequency of micronuclei in the lymphocytes. At the same
concentrations,strawberrytreeleafextractwasslightlymorecytotoxicthanarbutin.However,
arbutin, unlike the extract, caused delays in the lymphocyte cell cycle. The observed
antiproliferative effect merits further research, which should focus on other cell types, in
particulartumourcelllines.
Keywords:cytotoxicity,HPLC,lymphocytes,medicinalplants,primaryDNAdamage
P113
DETERMINATION OF ACUTE AND SUBLETHAL EFFECTS OF PERMETHRIN ON NARROW
CLAWEDCRAYFISH(AstacusleptodactylusEsch.1823)
A..KarasuBenli1,S.KayiranTunca1,P.Arslan2,G.Gl3,A.SepiciDinel4
1
Graduate School of Natural and Applied Sciences, Environmental Sciences Department, Gazi
University,Ankara,Turkey(benli@gazi.edu.tr)
2
BiologyDepartment,FacultyofScience,AnkaraUniversity,Ankara,Turkey
3
Health Services Vocational School, Environmental Health and Environmental Sciences
Program,GaziUniversity,Ankara,Turkey
4
FacultyofMedicine,GaziUniversity,Ankara,Turkey
In the present study, the acute and sublethal toxic effects of the synthetic pyhretroid
permethrin,atoxicpollutantonaquaticsystemsfromagriculturalactivities,onnarrowclawed
th
12.HBK25012 CBC
crayfish(Astacusleptodactylus)weredetermined.Theacute96hLC50valuewasestimatedas
0.091(95%CI=0.50422.2734)g/L.Staticbioassaysystemwasusedintworeplicates.1/10
of 96 h LC50 value (0.0091 g/L) was used as the sublethal exposure concentration and total
hemocytecountsandtissuemalondialdehyde(MDA)levelsweredeterminedafter48and96
hours.After96hsublethalexposuretopermethrin,totalhemocytecountsweresignificantly
increased (p < 0.05). The MDA levels of muscle and gill tissues were significantly increased
whilehepatopancreasMDAlevelsdecreasedafter96hsublethalpermethrinconcentrations(p
<0.05).Inconclusion,permethrinwasextremelytoxictonarrowclawedcrayfish,anontarget
butindicatorspecies,andaffectedbiomarkersofhealthsuchaslipidperoxidationlevelseven
atsublethalconcentrations.
Keywords:bioassay,permethrin
P114
CITOTOKSINI, GENOTOKSINI I CITOGENETIKI UINCI KLORPIRIFOSA NA LJUDSKE
LIMFOCITEPERIFERNEKRVIUUVJETIMAINVITRO
N.Kopjar,V.Kauba,R.Rozgaj,M.Mladini,M.Mili,D.eljei
Jedinica za mutagenezu, Institut za medicinska istraivanja i medicinu rada, Zagreb, Hrvatska
(nkopjar@imi.hr)
Klorpirifosjejedanodnajeekoritenihorganofosfornihinsekticida.Zbogvisokelipofilnosti,
molekulaklorpirifosalakoulaziucitoplazmuioteujestaninemakromolekule.Uuvjetimain
vitro istraili smo utjecaj klorpirifosa na apoptozu i nekrozu, oteenja DNA te citogenetika
oteenja u ljudskim limfocitima periferne krvi, izlaganim 4 i 24 h koncentracijama koje
odgovaraju:razininakojojjeuoentetanuinak,prihvatljivomdnevnomunosutereferentnoj
raziniizloenosti.Preivljenjestanicapraenojeprimjenomtestasfluorescencijskimbojama.
Primarna oteenja DNA procijenjena su komettestom, a citogenetika oteenja
mikronukleustestom. Klorpirifos pri testiranim koncentracijama izaziva porast smrtnosti
limfocita (najvie apoptozom), ovisno o koncentraciji i duini izlaganja. Rezultati komettesta
upuuju na koncentracijski ovisan porast razine primarnih oteenja DNA. Pomou
mikronukleustesta utvrena su znaajna oteenja kromosoma i diobenog vretena te
poremeaji stanine kinetike i proliferacije limfocita. Citotoksini, genotoksini i citogenetiki
uinciklorpirifosa,uoeniakiprinjegovimdoputenimkoncentracijama,pozivajunapoveani
oprez, osobito jer neke namirnice mogu sadravati visoke koncentracije ostataka ovog
pesticida. Stoga je, radi smanjenja rizika od izloenosti klorpirifosu, potrebno nastaviti
istraivanjasciljemboljegrazjanjenjamehanizamanjegovetoksinosti.
Kljunerijei:toksinost,oteenjaDNA,pesticid,ljudskekrvnestanice
EVALUATIONOFTHECYTOTOXIC,GENOTOXICANDCYTOGENETICEFFECTSOFCHLORPYRIFOS
INHUMANPERIPHERALBLOODLYMPHOCYTESINVITRO*
N.Kopjar,V.Kauba,R.Rozgaj,M.Mladini,M.Mili,D.eljei
Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia
(nkopjar@imi.hr)
Chlorpyrifosisoneofthemostwidelyusedorganophosphateinsecticides.Duetoitslipophilicity,
chlorpyrifos reaches the cytoplasm easily and damages cellular macromolecules. This in vitro
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12.HBK25112 CBC
study investigated the ability of chlorpyrifos to induce apoptosis/necrosis, DNA lesions and
cytogenetic damage in human lymphocytes treated for 4 and 24 h with chlorpyrifos at doses
equal to its observable effect level, acceptable daily intake, and reference exposure level.
Cytotoxicitywastestedusingaviabilityassaywithfluorescencedyes.LevelsofprimaryDNAand
cytogeneticdamagewerestudiedbycometassayandcytokinesisblockedmicronucleus(CBMN)
assay, respectively. Following treatment, lymphocyte viability decreased in a time and
concentrationdependentmanner.Apoptosisdominatedovernecrosis.ThelevelsofprimaryDNA
damage were also concentrationdependent, but longer exposure resulted in slightly lower
values,bothofcomettaillengthsandoftailDNA.ThefindingsofCBMNassayindicatesignificant
DNA damage, aneugenic potential, and disturbances in cellcycle kinetics. The fact that
chlorpyrifoswas,evenatconcentrationstolerableforhumanexposure,abletoinducesignificant
cytotoxic,genotoxicandcytogeneticeffectscallsforparticularattention,sincesomefooditems
oftencontainhighresiduallevelsofthispesticide.Inthissense,furtherstudiesaimedatclarifying
themechanismsinvolvedinchlorpyrifostoxicitytoagreaterextentareadvised.
Keywords:toxicity,DNAdamage,pesticide,humanbloodcells
Acknowledgement: This work was financially supported by the Project No. 8366 Organic Pollutants in
EnvironmentMarkersandBiomarkersofToxicity(OPENTOX),fundedbytheCroatianScienceFoundation.
P115
DETERMINATIONOFCELLDEATHMECHANISMONHUMANLUNGADENOCARCINOMACELLS
EXPOSEDTOCERANIB2
H.M.Kutlu1,D.Vejselova1,G.Ku2
1
Department of Biology, Faculty of Science, Anadolu University, Eskiehir, Turkey
(hmkutlu@anadolu.edu.tr)
2
DepartmentofHealth,FacultyofOpenEducation,AnadoluUniversity,Eskiehir,Turkey
There is a fact that resistance to widely used chemotherapeutics occurs frequently. Thus
novelagentsforcancertreatmentarerequired.Inthepresentstudywedeterminedthecell
deathmechanismofceranib2,aceramidaseinhibitor,treatedhumanlungadenocarcinoma
cells (A549). The 50% inhibition concentration (IC50) of ceranib2 on A549 cells was
determinedinourpreviousstudy,andusedforthetreatmentsinthepresentstudy.Thecell
deathanalysiswasrealisedonflowcytometrybyannexinVFITCandpropidiumiodide(PI)
staining. The percentage of apoptotic cells was high compared to that of necrotic cells.
Furthermore, the effect of ceranib2 on the mitochondrial membrane potential of the
treatedA549cellsfor24hours.SignificantchangeonthemitochondrialmembraneofA549
cells exposed to IC50 value of ceranib2 for 24 hours was recorded. All experiments were
realised in triplicates. In conclusion, ceranib2 was highly cytotoxic to human lung
adenocarcinomacellsinitsIC50 concentrationleadingtoapoptosisbychangingthepotential
ofthemitochondrialmembrane.
Keywords:Ceramide,celldeath,ceranib2,apoptosis,inhibitor
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P116
UINAK KADMIJA NA FOTOSINTEZU I SADRAJ SEKUNDARNIH METABOLITA U LIAJEVA
Parmeliasulcata,EverniaprunastriIElavoparmeliacaperata
A.Masla1,M.Tkalec1,M.Masla2
1
Sveuilite u Zagrebu, Prirodoslovnomatematiki fakultet, Bioloki odsjek, Botaniki zavod,
Zagreb,Hrvatska
2
Geonaturad.o.o.,Zagreb,Hrvatska(mmaslac@geonatura.hr)
Liajevisesvrstavajumeunajpoznatijebioindikatorekvalitetezraka.Utjecajoneienjazraka
oitujesefiziolokimpromjenama,aosjetljivostliajarazlikujeseizmeuvrsta.Sobziromdase
kadmij povezuje s oneienjem zraka iz prirodnih i umjetnih izvora, cilj istraivanja je bio
otkriti njegov uinak na fotosintezu i sekundarne metabolite liajeva. Koliina sekundarnih
metabolita esto se mijenja ovisno o prisutnosti tekih metala u talusu to ukazuje da ovi
spojeviimajuznaajnuuloguutolerancijinatekemetale.Uovomsmoradutrivrsteliajeva
(Parmelia sulcata, Evernia prunastri i Flavoparmelia caperata) izlagali otopini kadmija (50
g/ml) u laboratorijskim uvjetima te smo nakon 1., 3. i 8. dana odreivali uinkovitost
fotosinteze i sadraj sekundarnih metabolita. Vrijednosti koncentracija metabolita, usninske
kiseline (F. caperata) te salazinske kiseline i atranorina (P. sulcata), su dan nakon izlaganja
kadmiju znaajno porasle, nakon tri dana su se snizile, te ponovno znaajno porasle nakon
osam dana izlaganja, to ukazuje na njihovu ulogu u imobilizaciji kadmija kod izlaganja u
relativno kratkom trajanju. Rezultati pokazuju da su istraivane vrste relativno otporne na
kadmij i da sekundarni metaboliti imaju znaajnu ulogu u zatiti primarnog metabolizma od
negativnihuinakaovogmetalaiakojenakonizlaganjakadmijuutrajanjuod8danautvrena
smanjenauinkovitostfotosinteze.
Kljunerijei:kadmij,oneienje,kvalitetazraka,liajevi,HPLC,fotosinteza
A.Masla1,M.Tkalec1,M.Masla2
Lichensareoneofthemostcommonairqualitybioindicators.Airpollutioncausesphysiological
changesinlichens,butsensitivitytoairpollutionisspeciesspecific.Sincecadmiumfromnatural
andhumanactivitiesisrelatedtoairpollution,thegoalofourresearchwastorevealitsimpact
onphotosynthesisandsecondarymetaboliteproduction.Secondarymetabolitesaresensitiveto
heavy metal accumulation indicating that they can have an important role in heavy metal
tolerance. In this research three lichen species (Parmelia sulcata, Evernia prunastri and
Flavoparmeliacaperata)wereexposedtocadmium(50g/ml)inlaboratoryconditionsandafter
thefirst,thirdandeighthdayphotosynthesisefficiencyandsecondarymetabolitescontentwere
determined. The content of the metabolites, usnic acid (F. caperata) and salazinic acid and
atranorin (P. sulcata), increased a day after the cadmium exposure, then deceased after three
daysandincreasedagainaftereightdaysofexposure.Thisindicatestheroleofthesemetabolites
in cadmium immobilization when exposure is relatively short. Results show that researched
speciesarerelativelyresistanttocadmiumandthatsecondarymetaboliteshavesignificantrolein
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protectionofprimarymetabolismfromcadmiumnegativeimpactsalthoughnegativeimpacton
lichenphotosynthesiswasrecordedaftereightdaysoftheexposure.
Keywords:cadmium,airpollution,airquality,lichens,HPLC,photosynthesis
P117
POLLUTION BIOMONITORING OF THE RIVER MORACA AND LAKE SKADAR USING CAGED
MUSSELSUniosp.
A.Perovi1,B.ljuki1,S.Perovi1,M.rut2,G.Klobuar2
Prirodnomatematiki fakultet, Univerzitet Crne Gore, Podgorica,
(aperov@rc.pmf.ac.me)
2
Prirodoslovnomatematikifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
Crna
Gora
The aim of this study was environmental pollution assessment of the lower flow of the river
Moraca polluted by municipal and other waste waters. Assessment was done by applying
biomarkers, bioassays and measuring the Biotic Index (BI). To test the genotoxic impact of
pollutantsonlivingorganismsinthisarea,forthefirsttimeCometassayandmicronucleustest
wereappliedonhemocytesoffreshwatermusselUniosp.cageexposedat6sitesforaperiod
of3weeksduringsummer.Thesemethodscanprovideratheraccurateinformationonlevelof
DNAdamageevenifitisverylow.ThehighestDNAdamagedetectedbytheCometassaywas
at the site Collector (closest to the City of Podgorica), followed by location Grbavci, Vukovci
and Vranjina. The Comet assay showed better resolution in detecting pollution at chosen
locationsthenBIorphysicochemicalwateranalysis.ThisconfirmstheassumptionthatBIand
physicochemical water analysis are insufficient for a complete assessment of pollution in
aquatic ecosystems, mostly because the measurability of biological responses following the
chemicalcontaminationfallbelowanalyticaldetectionlimitsand/orafterchemicalexposure
hasfinished.Moreover,the factisthatCometassayandmicronucleustest correctlypointed
out the location polluted with industrial untreated wastewater justifies the introduction of
thesemethodsinthestandardpollutionmonitoringsystem.
P118
BIOLOKARAZGRADNJAORGANSKIHTVARIUVODIODABIRMETODEHRNENISO9439:2000
ILIHRNENISO10707:2000
L.Ulm,I.Hrga,A.Krivohlavek
Nastavnizavodzajavnozdravstvo"Dr.Andrijatampar",Zagreb,Hrvatska(lea.ulm@stampar.hr)
12.HBK25412 CBC
hlapivost, svojstvo adsorpcije, toksinost ispitivane tvari koja bi mogla inhibirati bakterijsku
aktivnostisl.),akojajedostupnautehnikojdokumentaciji.
Kljunerijei:biolokarazgradnja,metode,fizikalnokemijskekarakteristike
L.Ulm,I.Hrga,A.Krivohlavek
Dr.AndrijatamparTeachingInstituteofPublicHealth,Zagreb,Croatia(lea.ulm@stampar.hr)
Biologicaldegradationisprocessinthenaturewheremicroorganismspresentinsoilorwater
degrade organic substances to simpler molecules. Degraded organic substances serve as a
source of energy for microorganisms. Biological degradation laboratory tests have significant
role when estimating the influence of the substance under test to the environment.
Biodegradablesubstanceswillnothavelongtermandharmfulimpactontheenvironment,and
theprocessoftheirremovalfromtheenvironmentissimple.Laboratorytestsdefinemethods
to'measure'theorganicsubstancebiodegradability.Measurementscanincludedetermination
ofcarbon(IV)oxide,producedasafinaldegradationproduct;measurementofthedissolved
oxygen reduction due to consumption in degradation process, or some other measurable
parameter can be used. The final selection of biological degradation methods (HRN EN ISO
9439:2000 or HRN EN ISO 10707:2000) depends on chemical and physical properties of the
substance (water solubility, volatility, adsorption, toxicity of test compound according to
bacterialactivityetc.)availableintechnicaldocumentation.
Keywords:biologicaldegradationmethods,physicalandchemicalproperties
P119
INVESTIGATION OF CERANIB2 INDUCED CYTOTOXICITY ON HUMAN LUNG
ADENOCARCINOMACELLS
D.Vejselova1,H.M.Kutlu1,G.Ku2
1
Department of Biology, Faculty of Science, Anadolu University, Eskiehir, Turkey
(cananveyselova@gmail.com)
2
DepartmentofHealth,FacultyofOpenEducation,AnadoluUniversity,Eskiehir,Turkey
Theincidenceofcancerhasbeenexperiencingariseinrecentyears.Lungcancerisoneoftype
common cases in the World. In this study we aimed to evaluate the cytotoxic and apoptotic
effects of ceranib2 on human lung adenocarcinoma cells (A549). For the cytotoxicity testing
MTT(3(4,5dimethylthiazol2yl)2,5diphenyl2Htetrazoliumbromide)colorimetricassaywas
used. Stock solution of ceranib2 was prepared in dimethyl sulfoxide (DMSO) and further
diluted in fresh culture medium. A549 cells were exposed to concentrations of ceranib2
rangingfrom5to110Mfor24hours.ThepercentagesofviabilityandIC50concentrationof
ceranib2for24hoursweredetermined.ThemorphologicalalterationsonA549cellscaused
byIC50concentrationofceranib2for24hourswereintestigatedonconfocalmicroscope.For
confocalmicroscopy,A549cellsweredoublestainedwithacridineorangeandphalloidine.Asa
conclusion, ceranib2 was found to be highly cytotoxic to A549 cells for 24 hours in dose
dependentmanner.Furthermore,ceranib2causedsignificantalterationsonceranib2treated
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12.HBK25512 CBC
A549cellsin24hours.Consequentlyceranib2inhibitedtheproliferationofA549cellsleading
tomorphologicalchangesonthecellsindicatingapoptosis.
Keywords:Ceranib2,lungadenocarcinoma,cytotoxicity,sphingolipid,ceramidaseinhibitors
MIKROBIOLOGIJA
MICROBIOLOGY
P120
BEZNJIHSENEMOE:EUKARIOTSKIORGANIZMISTANOVNICIAKTIVNOGMULJA
A.M.Ladi,A.Mojsoviui
Zdravstvenoveleuilite,Zagreb,Hrvatska(amladis@zvu.hr)
Obrada otpadnih voda Grada Zagreba odvija se na Ureaju za proiavanje otpadnih voda
Grada Zagreba u dva stupnja, gdje je drugi stupanj bioloka obrada aktivnim muljem.
Razgradnja organske tvari u bioloki bazenima, koja je kljuan faktor uinkovitosti
proiavanja otpadne vode, odvija se uz pomo kisika koji se dovodi u bioloke bazene
upuhivanjematmosferskogzraka.Ciljistraivanjabiojeutvrditizastupljenostpojedinihrodova
eukariotskihorganizamaaktivnogmuljakaoiodnospremaodreenimabiotikimimbenicima.
Istraivanjejeprovedenotijekom2012.godine,odreivanjemeukariotskihmikroorganizamau
aktivnom mulju i njihove zastupljenosti tijekom pojedinih mjeseci. Ukupno je tijekom 2012.
godine identificirano 35 rodova. Najvia bioloka raznolikost zabiljeena je u sijenju, kada je
zabiljeen 31 rod eukariotskih mikroorganizama u aktivnom mulju od kojih je najzastupljeniji
biorodEpistylissp.,dokjenajniabiolokaraznolikostzabiljeenaulipnjukadajezabiljeen21
rod od kojih je najzastupljeniji bio rod Vorticella sp.. Rodovi Epistylis sp., Opercularia sp.,
Colurellasp.,Vorticellasp.,Rotariasp.,Amphileptussp.,Aspidiscasp.,Zoogleasp.iEuglypha
sp. bili su stalno zastupljeni tokom 2012. godine. Oscilacije u brojnosti mikroorganizama
aktivnog mulja pojedinih mjeseci ovise o utjecaju abiotikih faktora okolia, od kojih su
najznaajnijitemperatura,kisik,protoktekoliinaorgansketvariuulaznojvodi.
Kljunerijei:aktivnimulj,eukariotskimikroorganizmi,Zagrebakeotpadnevoded.d.
A.M.LadiiA.Mojsoviui
UniversityofAppliedHealthSciences,Zagreb,Croatia(amladis@zvu.hr)
WastewatertreatmentoftheCityofZagrebisperformedattheWastewaterTreatmentPlantof
the City of Zagreb in two stages, where the second stage involves biological treatment by
activatedsludge.Decompositionoftheorganicmatterinbiologicalpools,whichisthekeyfactor
of efficiency in wastewater treatment, is performed with the assistance of oxygen which is
conveyedtothebiologicalpoolsbyinsufflatingatmosphericair.Thepurposeofthestudywasto
establishrepresentationofindividualgeneraofeukaryoticorganismsofactivatedsludge,aswell
as the relationship toward specific abiotic factors. The study was conducted during 2012, by
determiningeukaryoticmicroorganismspresentintheactivatedsludgeandtheirrepresentation
during particular months. In total, there were 35 genera identified during 2012. The highest
biologicaldiversitywasrecordedinJanuary,when31generaofeukaryoticmicroorganismswere
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recorded in the activated sludge, where the genus Epistylis sp had the highest representation,
whilethelowestbiologicaldiversitywasrecordedinJune,when21generawasrecorded,with
thehighestoccurrenceofVorticellasp.genus.ThegeneraEpistylissp.,Operculariasp.,Colurella
sp.,Vorticellasp.,Rotariasp.,Amphileptussp.,Aspidiscasp.,Zoogleasp.andEuglyphasp.were
constantly represented during 2012. Oscillation in abundance of eukaryotic microorganisms in
activatedsludgeincertainmonthsdependsontheinfluenceofabioticenvironmentalfactors,the
most important being temperature, oxygen, flow and amount of organic matter in the inflow
water.
P121
HETEROTROFNEBAKTERIJEUSEDIMENTUIREGPODRUJASPLITA
E.iiGuo1,N.Krstulovi2,S.estanovi2
1
UdrugaBIOM,Zagreb,Hrvatska(elvira.zizicguso@gmail.com)
2
Laboratorijzamikrobiologiju,Institutzaoceanografijuiribarstvo,Split,Hrvatska
Iako ukupna debljina morskog sedimenta u pojedinim podrujima moe iznositi i do nekoliko
kilometara, bakterije naseljavaju uglavnom povrinske slojeve sedimenta. Postotak sedimenta
koji naseljavaju bakterije je manji od 1%. Glavni razlog za to je opadanje koliine organskog
materijala s poveanjem dubine sedimenta. Istraivanje brojnosti bakterija u sedimentu
provedeno je na irem podruju Splita, na postajama razliitog trofikog stupnja: Katelanski
zaljev, Gradska luka Split, Splitski kanal. Uzorci sedimenta su uzimani do dubine od 15 cm i
podijeljeni vertikalno na slojeve: 01 cm, 12 cm, 25 cm, 1015 cm. Uzorci morske vode su
uzimaninastandardnimoceanografskimdubinama(0,10,20,35m).Ciljistraivanjabiojeutvrditi
brojnostiraspodjeluheterotrofnihbakterijaposlojevimasedimenta,usporeditipromjenemeu
istraivanim postajama te odrediti odnos brojnosti bakterija vodeni stupac sediment. Brojnost
bakterijausedimentuodreenajemetodomdirektnogbrojenjaepifluorescentnimmikroskopom,
dokjebrojnostheterotrofnihbakterijauvodenomstupcuodreenametodomdirektnogbrojenja
protonim citometrom. Najvee prosjene vrijednosti brojnosti bakterija u sedimentu utvrene
su na postaji u Katelanskom zaljevu, a najmanje na postaji u Splitskom kanalu. Analizom
brojnosti bakterija na vertikalnoj skali u sedimentima ispitivanih postaja uoene su znaajne
razlikemeupostajama.BrojnostbakterijauKatelanskomzaljevubilajegotovoujednaenana
cijelojispitivanojdubini,dokjebrojnostbakterijausedimentugradskelukepravilnoopadalaod
povrine do dubine od 15 cm. Ovakva raspodjela brojnosti bakterija u sedimentu moe se
objasniti utjecajem rijeka i bioturbacijom, odnosno eutrofikacijom. Usporedbom brojnosti
bakterijavodenogstupcaisedimentazabiljeenesuznaajnoveeprosjenevrijednostibrojnosti
bakterijausedimentu,itozatriredaveliine.
Kljunerijei:brojnostbakterija,sediment,vodenistupac
HETEROTROPHICBACTERIAINSEDIMENTOFAWIDERAREAOFSPLIT
E.iiGuo1,N.Krstulovi2,S.estanovi2
1
AssociationBIOM,Zagreb,Croatia(elvira.zizicguso@gmail.com)
2
LaboratoryofMarineMicrobiology,InstituteofOceanographyandFisheries,Split,Croatia
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12.HBK25712 CBC
Although the total thickness of marine sediment in some areas can be up to several
kilometers, bacteria inhabit mainly the surface layers of the sediment. The percentage of
bacteriasettled sediment is less than 1%. The cause of this distribution is the decrease in
organicmaterialquantitywithincreasingsedimentdepth.Thesedimentbacteriaabundance
research was carried out in the wider area of Split, at stations of different trophic status:
Katela bay, Split harbour, Split channel. The sediment samples were taken down to the
depth of 15 cm and vertically divided into layers: 01 cm, 12 cm, 25 cm, 1015 cm. The
seawater samples were taken at standard oceanographic depths (0, 10, 20, 35 m). The
purpose of the research was to determine the bacteria abundance and the distribution
within the different sediment layers, to compare variations between the studied stations
and to define ratio of bacterial abundance between the water column and the sediment.
Epifluorescentmicroscopydirectcountingmethodwasusedtodeterminesedimentbacteria
abundance, and the flow citometry direct counting method to determine water column
heterotrophicbacteriaabundance.Thehighestsedimentbacteriaabundanceaveragevalues
werefoundattheKatelabaystation,thelowestattheSplitchannelstation.Analysingthe
bacterialabundanceonverticalscale,majordifferencesinsampledstationssedimentswere
noticedbetweenthestations.ThebacterialabundanceinKatelabaywasalmostconsistent
atstudieddepths,whilebacterialabundanceinSplitharboursedimentshowedaconsistent
declinefromthesurfacetothe15cmdepth.Theriverflow,bioturbationandeutrofication
influencecanexplainsuchsedimentbacteriadistribution.Comparingthewatercolumnand
sediment bacteria abundance, notably higher approximate vallues in sediment bacteria
abundance,eveninthreeordersofmagnitudehigher,wererecorded.
Keywords:bacterialabundance,sediment,watercolumn
KOMPARATIVNAFIZIOLOGIJA,IMUNOBIOLOGIJAIBIOLOGIJAOVJEKA
COMPARATIVEPHYSIOLOGY,IMMUNOBIOLOGYANDHUMANBIOLOGY
P122
ANIMALNI MODEL PSORIJAZE: ANTIUPALNA I ANTIOKSIDATIVNA UINKOVITOST
EPIGALOKATEHINGALATA
V.Balta,D.Odeh,M.Matakovi,M.Kunti,M.Kukolj,N.Oroli
Zavodzaanimalnufiziologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuiliteu
Zagrebu,Zagreb,Hrvatska(nada.orsolic@biol.pmf.hr)
Istrailismomoguekorisnodjelovanjepripravkaepigalokatehingalata(EGCG)naanimalnom
modelu psorijaze, prouzroene iritantom dinpropil disulfidom (PPD), tijekom 5 dana.
AntiupalnoiantioksidativnodjelovanjeEGCGapratilismoslijedeimmetodama:brojstanicau
trbunoj upljini, funkcionalna aktivnost makrofaga u peritonealnoj tekuini, patohistoloka
analizapromjenanakoipokusnihivotinja,hematolokiibiokemijskiparametrikrvitemetode
zaprocjenuantioksidativneuinkovitosti(reduciraniglutation,lipidnaperoksidacija).Rezultati
ukazuju da pripravak EGCGa smanjuje ukupan broj upalnih stanica u peritonealnoj tekuini
mieva.Hematolokiibiokemijskipokazateljikretalisuseunutarnormalnihvrijednostidokje
razina upalnih citokina u plazmi bila poveana u ivotinja obraenih s PPD i/ili zdruenim s
EGCGom, posebice, imbenik stimulacije kolonija granulocita (GCSF) i interleukina6 (IL6).
PatohistolokaanalizauzorakakoepokazalajedazdruenatopikaprimjenairitantasEGCG
ima upalnu reakciju znatno slabijeg intenziteta od PPDa. Genotoksini uinak istraivanih
pripravaka procjenili smo pomou komettesta i mikronukleustesta. Rezultati komettesta i
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12.HBK25812 CBC
Kljunerijei:mijimodelpsorijaze,konelezije,termografija,EGCG
V.Balta,D.Odeh,M.Matakovi,M.Kunti,M.Kukolj,N.Oroli
DivisionofAnimalPhysiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(nada.orsolic@biol.pmf.hr)
Keywords:mousemodelsofpsoriasis,skinlesions,thermography,EGCG
P123
SEPARACIJSKA ANKSIOZNOST U MLADUNACA TAKORA S PERINATALNO PROMIJENJENIM
METABOLIZMOMSEROTONINA
S.Blaevi1,M.Merkler1,D.Hamidovi2,D.Hranilovi1
1
Zavodzaanimalnufiziologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuilite
uZagrebu,Zagreb,Hrvatska(blazevic@biol.pmf.hr)
2
Dravnizavodzazatituprirode,Zagreb,Hrvatska
Serotonin (5HT) je biogeni amin prisutan u mozgu (sredinji odjeljak) i tjelesnim tkivima
(periferni odjeljak). 5HT regulira razvoj ivanog sustava i kao neurotransmiter posreduje
socijalno i anksiozno ponaanje. Kad su odvojeni od majke, mladunci takora proizvode
ultrazvune dozive koji ukazuju na nelagodu zbog gubitka kontakta i mogu sluiti kao mjera
anksioznosti. Posljedice perinatalnog poremeaja metabolizma serotonina istraili smo
tretirajui Wistar takore tijekom razvoja neposrednim prekursorom serotonina, 5
hidroksitriptofanom(5HTP,25mg/kg)kojipovisujesamoperifernerazine5HTiliinhibitorom
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monoaminoksidaze,tranilciprominom(TCP,2mg/kg),kojiznaajnopovisujerazine5HTuoba
odjeljka,od13.gestacijskogdo21.postnatalnogdana(PND).Dozivanjesmosnimali13.i16.
PND. Latenciju, broj i trajanje doziva koristili smo kao mjeru anksioznosti. Frekvenciju
maksimalne energije koristili smo kao mjeru sazrijevanja. Poveana razina anksioznosti i
odgoeno sazrijevanje bilo je blago i neznaajno nakon tretmana 5HTPom, ali snano i
znaajno nakon tretmana TCPom. Ovi rezultati pokazuju da poviene razine serotonina
tijekom razvoja mozga uzrokuju poremeaje ponaanja koji su izraeniji nakon promjene
homeostaze 5HT u oba odjeljka i sugeriraju da sama hiperserotoninemija nije dovoljna da
uzrokujeporemeajevezaneuz5HTuneurorazvojnimsindromima.
Kljunerijei:serotonin,ultrazvunevokalizacije,hiperserotoninemija,tranilcipromin,takori,
5hidroksitriptofan
SEPARATIONANXIETYINRATPUPSWITHPERINATALLYALTEREDSEROTONINMETABOLISM
S.Blaevi1,M.Merkler1,D.Hamidovi2,D.Hranilovi1
1
DivisionofAnimalPhysiology, DeparmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(blazevic@biol.pmf.hr)
2
StateInstituteforNatureProtection,Zagreb,Croatia
Inmammals,serotonin(5HT)ispresentbothinthebrain(centralcompartment)andperipheral
tissues(peripheralcompartment).5HTregulatesneurodevelopmentandasaneurotransmitter
mediatesessentialbehaviorsincludingsocialandanxietybehaviors.Ratpupsseparatedfrom
theirmotherproduceultrasoniccallswhichindicatedistressfromlossofaffiliatecontactand
mayserveasameasureofanxiety.Effectsofperinataldisturbancesin5HTmetabolismwere
studiedbyexposingdevelopingWistarratstotreatmentswiththeimmediate5HTprecursor5
hydroxytryptophan (5HTP, 25 mg/kg), which significantly raises only peripheral 5HT
concentrations, or the monoamine oxidase inhibitor tranylcypromine (TCP, 2 mg/kg), which
significantly elevates 5HT in both compartments, from gestational day 12 until postnatal day
(PND)21.SeparationcallswererecordedonPNDs13and16.Latency,numberanddurationof
callswereusedasmeasuresofanxiety.Frequencyofmaximalenergywasusedasameasureof
maturation. Increase in separation anxiety and delay in maturation were slight and non
significant after 5HTP treatment but strong and significant after TCP treatment. The results
indicatethatexposureofdevelopingbraintoincreased5HTconcentrationsleadstobehavioral
abnormalities that were more pronounced after perinatal alteration in both 5HT
compartments, indicating that hyperserotonemia alone is not sufficient to cause 5HTrelated
disturbancesinneurodevelopmentalsyndromes.
P124
RAZLIKEUSASTAVUMASNIHKISELINAUMURINE,UGORAIJEGULJEIZJADRANSKOGMORA
BLIZUDUBROVNIKA
D.iki1,R.Fuchs2,S.MatiSkoko3,D.Skaramuca1,Z.Frani2,I.Landeka4,B.Skaramuca5
1
Zavodzaanimalnufiziologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuilite
uZagrebu,Zagreb,Hrvatska(domagoj.djikic@biol.pmf.hr)
2
Institutzamedicinskaistraivanjaimedicinurada,Zagreb,Hrvatska
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12.HBK26012 CBC
Institutzaoceanografijuiribarstvo,Split,Hrvatska
Prehrambenotehnolokifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
5
Odjelzaakvakulturu,SveuiliteuDubrovniku,Dubrovnik,Hrvatska
Omjer6/3masnihkiselinauprehraniinjihoviuincinazdravljeljudidobrosupoznati.
Prehranaribomvaanjeizvorrazliitihmasnihkiselina.Sastavmasnihkiselinauribamaizravno
je povezan s raznolikosti organizama koji prevladavaju u prehrani pojedinih vrsta riba. U
prethodnim radovima pokazali smo razlike u sastavu prehrane izmeu predatorskih vrsta
murineiugora,auovomraduanaliziramorazlikeusastavumasnihkiselinaizmeudvijevrste.
Rezultatisuusporeeniisasastavommastiuobinejeguljejerjesastavmasnihkiselinaute
vrstedobropoznat.SvesuribeprikupljeneuJadranskommoruiribljeuljeje ekstrahiranoiz
miinogtkiva(n=6/vrsti).Analizamasnekiselinenapravljenajeplinskomkromatografijom.
Rezultati su pokazali najveu koliinu proteina u murine, slino jegulji, a najmanju koliinu u
ugora. Najvee koliine ekstrahiranih masti zabiljeene su u jegulje i murine, dok je ukupna
koliina masti bila izrazito nia u ugora. U jegulja je zabiljeen najvei omjer
zasienih/nezasienih masti, najvii omjer 6 / 3 masnih kiselina i najvii postotak
viestruko nezasienih masnih kiselina (% PUFA) u odnosu na murinu ili ugora koji su imali
sline vrijednosti u ova tri parametra. Meutim, postotak jednostruko nezasienih masnih
kiselina(%MUFA)biojeslianumurineiugora,iviinegoujegulje.Overazlikeupuujuda
razliitaekofiziologijairazliitisastavprehraneistraenihsrodnihvrstaribautjeunarazliiti
sastavmasnoaunjihovimtkivima.
Kljunerijei:masnekiseline,fiziologijaprehrane,ekofiziologija
DIFFERENCESINFATTYACIDCOMPOSITIONBETWEENMORAY,CONGERANDCOMMONEEL
FROMADRIATICSEANEARDUBROVNIK
D.iki1,R.Fuchs2,S.MatiSkoko3,D.Skaramuca1,Z.Frani2,I.Landeka4,B.Skaramuca5
1
DivisionofAnimalPhysiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(domagoj.djikic@biol.pmf.hr)
2
InstituteforMedicalResearchandOccupationalHealth,Zagreb,Croatia
3
InstituteofOceanographyandFisheries,Split,Croatia
4
FacultyofFoodTechnologyandBiotechnology,UniversityofZagreb,Zagreb,Croatia
5
DepartmentofAquaculture,UniversityofDubrovnik,Dubrovnik,Croatia
Ratioof6/3fattyacidinthehumannutritionandtheirbeneficiaryeffectsonhealtharewell
known.Fishconsumptionisanimportantsourceoffattyacids.Thecompositionoffattyacidsin
fishisdirectlylinkedtothediversityoforganismsdevouredbyparticularfishspecies.Previously
we showed the differences in pray composition between toporder predators the Moray and
Conger eel and here present the differences in fatty acid composition between two species.
ResultsarecomparedtoCommoneelsinceinthisspeciesafattyacidcompositioniswellknown.
All fish were collected in the Adriatic sea and the fish oil was extracted from muscular tissue
(N=6/species).Thefattyacidanalysiswasdonebygaschromatography(GC).Thisworkisthefirst
existingreportonMorayeelfattyacidcomposition.TheresultsshowedthatMorayhadhighest
amountofprotein,similartoCommoneelandfollowedbyCongereel.Totalfatcontentwasthe
highestinCommoneel,followedbyMorayanddistinctivelylowerinConger.Commoneelhad
the highest saturated/unsaturated ratio, the highest 6/3 fatty acids ratio and the highest
percentage of polyunsaturated fatty acids (PUFA%) compared to Moray or Conger which had
similar values in these threeparameters. However, percentage of monounsaturated fatty acids
(MUFA%)washigherandsimilarinMorayandCongereelthaninCommoneel.Thesedifferences
4
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implicatethatdifferentecophysiologyandpraycompositioninfluencethefatcompositioninkin
Anguilliformspecies.
Keywords:fattyacids,nutritionalphysiology,ecophysiology
P125
UINKOVITOST KAFEINSKE KISELINE NA PERITONEALNU ANGIOGENEZU U EHRLICHOVOM
ASCITESNOMTUMORU
M.Kukolj,M.Kunti,R.Raan,E.Roak,N.Oroli
Zavodzaanimalnufiziologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuiliteu
Zagrebu,Zagreb,Hrvatska(nada.orsolic@biol.pmf.hr)
Napredovanjerazvojatumorskihstanicaukljuujepreivljavanje,irenje,invaziju,angiogenezu
imetastaziranje.Kafeinskakiselina(CA)jebiolokiaktivnasastavnicapropolisakojapokazuje
antioksidativna, protuupalna, antiproliferativna, citostatska, antiangiogena i antineoplastina
svojstva. U ovom radu istraivali smo uinak kafeinske kiseline na rast tumora i tumorsku
angiogenezuumievanositeljaEhrlichovogascitesnogtumora(EAT).StaniceEAT(2,5x106)su
injicirane intraperitonealno (i.p.) u Swiss albino mieve. Nakon inokulacije tumora, mievi su
injiciranii.p.CAudoziod40i80mgkg1tjelesneteineufazieksponencijalnograstaod5dana
nakon injekcije stanica tumora (na dan 5, 7, 9). etrnaesti dan analizirali smo ukupni broj
stanica ascitesa u peritonealnoj upljini, volumen ascitesa, diferencijalnu analizu stanica
prisutnih u peritonealnoj upljini, funkcionalna aktivnost makrofaga, NO i proiljenost
peritonealne stijenke. CA je inhibirala rast stanica EAT i nastanak ascitesa u peritonealnoj
upljini mieva nositelja EAT. Nadalje, rezultati smanjenja peritonealne angiogeneze i
mikroilne gustoe pokazuju antiangiogeni potencijal CA in vivo. CA smanjuje razinu NO u
stanicama EAT, dok razina NO je poveana u peritonealnim makrofagima. Sve to upuuje na
zakljuakdaCAmoeaktiviratimakrofageipoveatinjihovcitotoksiniuinakkrozpoveanu
proizvodnjuNOtesprijeitirasttumoraiangiogenezu.
Kljunerijei:Ehrlichovascitesnitumor,kafeinskakiselina,antiangiogeneza,NO,makrofagi:M1,M2i
TAMs
M.Kukolj,M.Kunti,R.Raan,E.Roak,N.Oroli
DivisionofAnimalPhysiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(nada.orsolic@biol.pmf.hr)
Progressionoftumorcelldevelopmentinvolvessurvival,proliferation,invasion,angiogenesis,
and metastasis. Caffeic acid (CA) is an active component of propolis extract, which exhibits
antioxidant, antiinflammatory, antiproliferative, cytostatic, antiangiogenic and most
improtantly, antineoplastic properties. In the present study we investigated the effect of of
caffeic acid on tumor growth and tumor angiogenesis in mice bearing Ehrlich ascites tumor
(EAT). EAT cells (2.5x106) were implanted intraperitoneally (i.p.) in Swiss albino mice. After
tumor inoculation, mice were injected i.p. with CA at dose of 40 and 80 mg kg1 bw in
exponentialgrowthphasefromthe5daysaftertumorcellinjection(onday5,7,9).Onday14,
ascites volume, the total number of cells, differential count of the cells present in the
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12.HBK26212 CBC
peritonealcavity,functionalactivityofmacrophages,NOandantiangiogenicparameterswere
determined.ThegrowthofEATcellsandformationofascitesintheperitoneumofEATbearing
mice was inhibited by CA. Further, results on decrease in the peritoneal angiogenesis and
microvesseldensityshowtheantiangiogenicpotentialofCAinvivo.CAdecreasedNOlevelin
tumor cells whereas NO level was increased in peritoneal macrophages. Taken together, we
conclude that CA may increase the cytotoxic actions of macrophages by increasing NO and
inhibittumorgrowthandangiogenesis.
Keywords:Ehrlichascitestumor,caffeicacid,antiangiogenesis,NO,macrophages:M1,M2andTAMs
P126
GALNA KISELINA SMANJUJE TUMORSKI RAST I ANGIOGENEZU U MIEVA S EHRLICHOVIM
ASCITESNIMTUMOROM
M.Kunti,M.Kukolj,N.Oroli
Zavodzaanimalnufiziologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuiliteu
Zagrebu,Zagreb,Hrvatska(nada.orsolic@biol.pmf.hr)
Galna kiselina (GA), takoer poznata kao 3,4,5trihidroksibenzojeva kiselina, je fenolna kiselina
prisutna u biljkama diljem svijeta, ukljuujui i ljekovito bilje Hrvatske. Ranija su istraivanja
pokazala citotoksine uinke galne kiseline na odreene vrste stanica raka bez oteenja
normalnih stanica. Cilj ovog istraivanja bio je utvrditi moe li galna kiselina inhibirati rast
Ehrlichovogascitesnogtumora(EAT)umia.StaniceEAT(2,5x106)suinjiciraneintraperitonealno
(i.p.)uSwissalbinomieve,kojisusljedeegdanapoelidobivatiGAi.p.udoziod40i80mgkg1
tijekom 10 dana. etrnaestog dana odreeni su volumen ascitesa, ukupni broj stanica u
peritonealnojupljini,gustoakrvoilja,funkcionalnaaktivnostmakrofagaiproizvodnjaduikovog
oksida(NO).Obrada s GAnormalizira razinuVEGFa u ascitesu i smanjuje neoangiogenezu, to
posljedinosmanjujerasttumoraipoboljavapreivljavanjeivotinja.ivotinjeobraenesGAu
dozi od 40 mg kg1 razvijaju manji volumen ascitesa u odnosu na kontrolu, to upuuje na
smanjenuvaskularnupermeabilnostismanjenjeVEGFekspresijeustanicamaEAT.
Kljunerijei:galnakiselina,rasttumora,angiogeneza,VEGF
GALLIC ACID REDUCES TUMOR GROWTH AND ANGIOGENESIS IN MICE WITH EHRLICH
ASCITESTUMOR
M.Kunti,M.Kukolj,N.Oroli
DivisionofAnimalPhysiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(nada.orsolic@biol.pmf.hr)
Gallic acid, also known as 3,4,5 trihydroxybenzoic acid, is a type of phenolic acid, which is
presentinplantsworldwide,includingCroatianmedicinalherbs.Gallicacidhasbeenshownto
havecytotoxiceffectsincertaincancercells,withoutdamagingnormalcells.Theobjectiveof
the present study was to determine whether gallic acid is able to inhibit growth of Ehrlich
ascitestumor(EAT)inmice.EATcells(2.5x106)wereimplantedintraperitoneally(i.p.)inSwiss
albinomice.Onedayaftertumorinoculation,micewereinjectedi.p.withGAatdoseof40and
80 mg kg1 bw during 10 days. On day 14, ascites volume, the total number of cells in the
peritoneal cavity, microvessel density, functional activity of macrophages and nitric oxid
production (NO) were determined. GA therapy normalizes VEGF in ascites and decreases
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12.HBK26312 CBC
neoangiogenesis,therebyreducingtumorgrowthandimprovingsurvival.GAtreatedanimals
at dose of 40 mg kg1 developed ascites in a smaller volume compared to the control,
suggestingdecreasedvascularpermeabilitybyreducingVEGFexpressioninEATcells.
Keywords:gallicacid,tumorgrowth,angiogenesis,VEGF
P127
POVEZANOST IVOTNIH NAVIKA I PRIMARNIH OTEENJA DNA SPERMIJA S
REPRODUKTIVNOMSPOSOBNOSTIMUKARACA
A.evo1,S.ogori2,P.Stani3,A.HorvatKneevi1,V.Benkovi1
1
Zavodzaanimalnufiziologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuilite
uZagrebu,Zagreb,Hrvatska(sevoan@me.com,vesna@biol.pmf.hr)
2
Poliklinikakvorc,Samobor,Hrvatska
3
Zavod za humanu reprodukciju, Klinika za enske bolesti i porode, KBC Zagreb, Zagreb,
Hrvatska
Kljunerijei:spermiji,oteenjaDNA,komettest,ivotnenavike
RELATIONSHIP BETWEEN LIFESTYLE HABITS AND PRIMARY DNA DAMAGE OF THE SPERM
ANDREPRODUCTIVEABILITYOFMEN
A.evo1,S.ogori2,P.Stani3,A.HorvatKneevi1,V.Benkovi1
1
DivisionofAnimalPhysiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(sevoan@me.com,vesna@biol.pmf.hr)
2
Polyclinickvorc,Samobor,Croatia
3
Division of Human Reproduction, Department of Gynaecology and Obstetrics, University
HospitalCentreZagreb,Zagreb,Croatia
Spermatogenesisandspermqualitydependongeneticpredisposition,physicaltrauma,and
numerous exogenous and endogenous factors in the testicles and surrounding tissue and
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12.HBK26412 CBC
organsthatarepartofthereproductivesystem.Theaimofthisstudywastodeterminehow
common method of analysis of sperm (motility, concentration and sperm morphology) are
associated with the analysis of DNA fragmentation of sperm, and the connection between
lifestylehabits(consumptionoftobaccoproducts,softdrugsandalcohol)ofrespondentsto
the quality of sperm. The analysis of seminal parameters: sperm concentration, sperm
motility and sperm morphology was performed according to the guidelines of the World
HealthOrganization(WHO).TodeterminetheprimaryDNAdamagespermcometassaywas
used. We analysed the interrelation of the sperm parameters, as well as the connection
between lifestyle. DNA damage of sperm was not significantly associated with traditional
seminal parameters (concentration, motility and sperm morphology and age of the
respondents). Consuming more than ten cigarettes a day, as well as consuming cannabis
more than three times a week, do not significantly affect any of the seminal parameters.
Drinking more than two alcoholic drinks per day significantly reduces the progressive
motility and sperm concentration. At the same time consuming more than two alcoholic
drinks per day and more than ten cigarettes a day significantly reduces the progressive
spermmotility.
Keywords:sperm,DNAdamage,cometassay,lifestylehabits
P128
UINKOVITOSTKVERCETINANABIOMARKEREZAOSTEOPOROZU
N.Oroli1,D.iki1,J.Nemrava2,P.Majcen1,M.Kunti1,M.Kukolj1,E.Roak1,E.Golua3
1
Zavodzaanimalnufiziologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuilite
uZagrebu,Zagreb,Hrvatska(nada.orsolic@biol.pmf.hr)
2
Klinikakirurgijeihitnemedicine,OpabolnicaZabok,Hrvatska
3
Odjelzaanesteziologiju,reanimatologijuiintenzivnumedicinu,KlinikibolnikicentarZagreb,
Zagreb,Hrvatska
Oksidativnistresmoedovestidoopsenoggubitkakotanemaseiizazvatilomnostkostiju,
kao kljune znaajke osteoporoze. Istraili smo zatitni uinak kvercetina (QU) na kosti u
osteoporoziizazvanojretinoinomkiselinom(13cRA)utakorateuinakQUnakostizdravih
ivotinja. Analizirali smo mineralnu gustou kostiju (BMD), serumske biljege kotane
pregradnje(osteokalcin;OCiCterminalnogfragmentatipaIkolagena;CTX),ukupnisadraj
kalcija (Ca) i fosfora (P) te geometrijska i fizikalna obiljeja bedrenih kostiju, histologiju,
hematolokeibiokemijskeparametare,lipidnuperoksidaciju(MDA)irazinuglutationa(GSH)
ustanicamajetreibubrega.Retinoinakiselinaprimjenjenaudoziod80mgkg1uzrokovala
je znaajan porast biokemijskih parametara, MDA, OC i CTX vrijednosti i znaajan pad
vrijednostiBMD,CaiPubedrenimkostimaterazineGSHujetriibubrezimauusporedbis
kontrolom. Intragastrina primjena QU (100 mg kg1) istodobno s 13cRA rezultirala je
znatnimsmanjenjemvrijednostiCTXiMDAuodnosunaRMO.QUjepoluiopozitivneuinke
na razinu Ca i P, BMD u distalnom dijelu femura, teinu i duinu femura, biokemijske
odredniceirazinuGSH.Istovremenaobradatakoras13cRAialendronatomimalajetoksini
uinakirezultiralaznaajnimsmanjenjemtjelesnemaseod5,67%nakondvatjednaobrade
ivotinja.ZatitnadjelotvornostQUprotivtoksinostiinduciranes13cRAutakoradajenadu
dabikvercetinmogaoimatislianzatitniuinaknakostiuljudi.
Kljunerijei:retinoinakiselina,osteoporoza,kvercetin,alendronat,takor
THEEFFECTIVENESSOFQUERCETINONBIOMARKERSFOROSTEOPOROSIS
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12.HBK26512 CBC
N.Oroli1,D.iki1,J.Nemrava2,P.Majcen1,M.Kunti1,M.Kukolj1,E.Roak1,E.Golua3
1
Division of Animal Physiology, Department of Biology, Faculty of Science, University of
Zagreb,Zagreb,Croatia(nada.orsolic@biol.pmf.hr)
2
Department of Surgery and Emergency Medicine, General Hospital Zabok, Zabok, Croatia
3
DepartmentofAnaesthesiology,ReanimatologyandIntensiveMedicine,UniversityHospital
CentreZagreb,Zagreb,Croatia
Oxidativestressmayleadtoextensivebonelossandskeletalfragility,twocharacteristicsof
osteoporosis. We studied the boneprotective effect of quercetin (QU) in the retinoic acid
inducedmodelofosteoporosis(RMO)andinhealthyrats.Densitometry,osteocalcin(OC),C
terminal crosslinked telopeptide (CTX), calcium (Ca) and phosphorus (P) content of bone
femur, geometric measurement on the femur, histology, hematological and biochemical
parameters as well as lipid peroxidation (MDA) and glutathione levels (GSH) of liver and
kidney cells were applied to all groups. Retinoic acid administration (80 mg kg1) revealed
significant increase in biochemical parameters, MDA level, OC and CTX values and a
significant decrease in values of BMD, Ca and P in the femur and GSH levels in liver and
kidney compared to control. Administration of QU intragastrically (100 mg kg1 bw) daily
concomitantwithretinoicacidresultedinsignificantlyreductionofCTXvaluesandMDAin
comparisontoRMO.QUalsoexertedpositiveeffectsonbiochemicalparameters,valuesof
Ca and P, BMD values in distal part, femoral weight and length and the GSH level.
Concomitant treatments of rats with retinoic acid and alendronate had toxic effect to
animals and resulted in a significant decrease in body mass of 5.67% after two weeks. The
protective role of the QU against the toxicity of retinoic acidinduced osteoporosis in rats
givesahopethatQUmayhavesimilarprotectiveactioninhumans.
Keywords:retinoicacid,osteoporosis,quercetin,alendronate,rat
P129
INHIBICIJA POLARIZACIJE MAKROFAGA KAFEINSKOM KISELINOM: MEUODNOS ROS,
ANTIOKSIDANSAIUPALNIHCITOKINA
N.Oroli,M.Kunti,M.Kukolj,I.oli
Zavodzaanimalnufiziologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuiliteu
Zagrebu,Zagreb,Hrvatska(nada.orsolic@biol.pmf.hr)
Makrofagisuvanestanicepriroenogisteenogimunosnogodgovorakojesenakonaktivacije
mogu diferencirati u M1 ili M2 fenotip. M1 makrofagi posjeduju proupalnu, mikrobicidnu i
antikancerogenu djelotvornost; M2 makrofagi i njima srodni tumorupridrueni makrofagi
(TAMs)sudjelujuuremodeliranjutkiva,angiogeneziiimunomodulaciji.Proizvodnjareaktivnih
kisikovihspojeva(ROS),kritinazaaktivacijuifunkcijuM1makrofaga,nunojepotrebnaiza
diferencijaciju M2 makrofaga i TAMs. Poznato je da obrada s antioksidansima blokira
diferencijaciju TAMs i tumorogenezu u mijem tumorskom modelu. Da bi prouili kako
kafeinskakiselina(CA),prirodniantioksidans,utjeenafunkcijuipolarizacijumakrofagaterast
tumora, mievi su injicirani sa stanicama Ehrlichovog ascitesnog tumora (EAT) i obraeni
tijekom 10 dana s kafeinskom kiselinom u dozi od 40 i/ili 80 mg kg1. Polarizacija makrofaga
dalje je potvrena prema razini izluenih pro i antiupalnih citokina. Kafeinska kiselina moe
poveaticitotoksinuaktivnostmakrofagaiinhibiratirasttumora.InhibicijskadjelotvornostCA
na TAMs mogla bi se zasnivati na njenoj antioksidacijskoj aktivnosti. Temeljem navedenog,
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12.HBK26612 CBC
N.Oroli,M.Kunti,M.Kukolj,I.oli
DivisionofAnimalPhysiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(nada.orsolic@biol.pmf.hr)
Macrophagesareimportantbothtoinnateandadaptiveimmuneresponsesandareknownto
differentiate into M1 or M2 phenotypes following activation. While M1 macrophages are
highlyproinflammatory,microbicidalandanticancer;M2macrophagesandtherelatedtumor
associatedmacrophages(TAMs)regulatetissueremodelingandangiogenesisandcandisplay
immunomodulatory activity. It is demonstrated that production of reactive oxygen species
(ROS), critical for the activation and functions of M1 macrophages, is also necessary for the
differentiation of M2 macrophages and TAMs, and that antioxidant therapy blocks TAMs
differentiation and tumorigenesis in mouse models of cancer. In order to study how caffeic
acid, a natural antioxidant, affects macrophage function, polarization and tumor growth, we
injectedmicewithEhrlichascitestumor(EAT)cellsandtreatedthemfor10dayswithcaffeic
acidinadoseof40and/or80mgkg1.Macrophagepolarizationwasfurthercharacterizedby
quantifying secreted pro and antiinflammatory cytokines. Caffeic acid may increase the
cytotoxicactionsofmacrophagesandinhibittumorgrowth;inhibitoryactivityonTAMsmaybe
mediatedthroughitsantioxidativeactivity.Takentogether,weconcludethatthecontinuous
administrationoftheROSinhibitorcaffeicacidefficientlyblockedtheoccurrenceofTAMsand
markedlysuppressedtumorigenesisinmousecancermodels.TargetingTAMsbyblockingROS
canbeapotentiallyeffectivemethodforcancertreatment.
Keywords:tumor,macrophagepolarization,caffeicacid,oxidativestress,immunomodulation
P130
UINAK FLAVONOIDA I HIPERTERMALNE INTRAPERITONEALNE KEMOTERAPIJE (HIPEC) NA
RASTTUMORAIINDUKCIJUMIKRONUKLEUSAUMIJEMMODELUTUMORA
N.Oroli1,M.Kunti1,M.Kukolj1,D.Sirovina2
1
Zavodzaanimalnufiziologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuilite
uZagrebu,Zagreb,Hrvatska
2
Zoologijski zavod, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska
Hipertermija pojaava klastogenost alkilirajuih spojeva. Istraili smo mogu li kvercetin (QU) ili
naringenin(NAR)senzibiliziratiEhrlichovascitesnitumor(EAT)nahipertermalnuintraperitonealnu
kemoterapiju (HIPEC) s cisplatinom te mogu li flavonoidi u kombinaciji s cisplatinom smanjiti
cisplatinom prouzroen broj mikronukleusa u retikulocitima periferne krvi mieva. QU i NAR su
injiciranimievima7i3danaprijeunosastanicaEAT,dokjecisplatina(5ili10mgkg1)injicirana
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mievimaintraperitonealnoprinormalnimilihipertermalnimuvjetima3dananakonunosastanica
EAT(2106).NaeistraivanjepodupiretvrdnjudaQUiNARuzdruenomlijeenjuscisplatinom
imajupotencijalzainhibiranjerastatumoraunormalnimihipertermalnimuvjetimatesmanjuju
broj mikronukleusa u retikulocitima periferne krvi mieva u normalnim uvjetima, dok pri
hipertermalnimuvjetimaflavonoidipojaavajuklastogenostcisplatine.
Kljunerijei:hipertermija,kemoterapija,flavonoidi,Ehrlichovascitesnitumor,genotoksinost
N.Oroli1,M.Kunti1,M.Kukolj1,D.Sirovina2
1
DivisionofAnimalPhysiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia
2
DivisionofZoology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,Zagreb,Croatia
Keywords:hyperthermia,chemotherapy,flavonoids,Ehrlichascitestumour,genotoxicity
P131
ZATITNI I REPARACIJSKI UINAK PENTADEKAPEPTIDA BPC 157 NA STANICE KRVI, JETRE I
BUBREGAMIA
N.Oroli1,M.Kunti1,M.Kukolj1,D.Sirovina2,I.opf3
1
Zavodzaanimalnufiziologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuilite
uZagrebu,Zagreb,Hrvatska(nada.orsolic@biol.pmf.hr)
2
Zoologijski zavod, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska
3
Bayerd.o.o.,Zagreb,Hrvatska
Istraili smo zatitni i reparacijski uinak pentadekapeptida BPC 157 na stanice krvi, jetre i
bubregamianakonoteenjaprouzroenogcisplatinom(CP).Mievesmopodijeliliu4skupine:
kontrola, BPC 157, BPC 157 i CP te CP. Pratili smo oteenje DNA stanica krvi, jetre i bubrega
nakon0,1,6i24sata,tebrzinupopravkaDNA.Rezultatikomettestapokazalisudajenajvee
oteenjeDNAprouzroilaCPnakon1satausvimanaliziranimtkivima,apostotakoteenjabio
jevidljivinakon24satatoupuujenavelikbrojdvostrukihlomova.Najveibrojapoptotinih
stanicanaenjeustanicamabubrega.BPC157prouzroiojeoteenjaDNAnakon1i6satito
ukazujenamogunoststvaranjaoksidativnogstresaulaskomBPC157ustanicu.Nakon24sata
dolojedopotpunogoporavkastanica.ZajednikaprimjenaBPC157iCPpokazalajesmanjenje
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12.HBK26812 CBC
Kljunerijei:cisplatina,BPC157,mi,mikronukleus,komettest
N.Oroli1,M.Kunti1,M.Kukolj1,D.Sirovina2,I.opf3
1
Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
(nada.orsolic@biol.pmf.hr)
2
DivisionofZoology,FacultyofScience,UniversityofZagreb,Zagreb,Croatia
3
Bayerd.o.o.,Zagreb,Croatia
WeinvestigatedtheprotectiveandreparativeeffectofBPC157onblood,liverandkidneycells
of mouse, after cisplatin (CP) treatment. The mice were divided into 4 groups: control, BPC
157, BPC 157 and CP and CP. We monitored the levels of DNA damage in blood, liver and
kidneycellsafter0,1,6,and24handthespeedofDNArepair.Thecomettestshowsthatthe
DNA was mostly damaged by CP after 1 h in all analysed tissues and the % of damage was
visibleafter24h,whichindicatedalargenumberofdoublestrandbreaks.Thehighestnumber
ofapoptoticcellswasfoundinkidneycells.BPC157causedtheDNAdamageafter1and6has
aresultofoxidativestressincurredbyBPC157administration.Thevaluesreturnedtonormal
after 24 h. The combined treatment with BPC 157 and CP resulted in lower CPcaused DNA
damage. A number of micronuclei, which is a doublestrand break indicator, in the CP group
increasedproportionallytotheperiodofexposureandhadthehighestvalueafter24h.The
combinedtreatmentwithBPCandCPdecreasedthemicronucleicount.Itwasshownthatthe
most sensitive organ to the toxic effects of CP was the kidney, followed by liver and
lymphocytes.ResultsindicatedthatBPC157hasaprotectiveeffectonhealthycells,whichis
possiblybasedonareducedintakeofCPintothecellorBPC157participationintheprocessof
DNArepairindamagedcells,whichrequiresfurtherresearch.
Keywords:cisplatin,BPC157,mouse,micronucleustest,comettest
P132
KVERCETIN SMANJUJE IRITACIJU KOE INDUCIRANU HEKSILSALICILATOM ILI DINPROPIL
DISULFIDOM:OKSIDATIVNISTRESIUPALA
N.Oroli,D.Odeh,M.Matakovi,V.Balta,M.Kunti,M.Kukolj,K.Sailo,D.iki
Zavodzaanimalnufiziologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuiliteu
Zagrebu,Zagreb,Hrvatska(nada.orsolic@biol.pmf.hr)
12.HBK26912 CBC
termografskomkamerom,analizomukupnogbrojaupalnihstanicaidiferencijalnomanalizom
stanica u peritonealnoj upljini, praenjem indeksa makrofagnog irenja, hematolokih i
biokemijskih parametara, uestalosti pojave mikronukleusa u retikulocitima, lipidne
peroksidacijeiglutationautkivukoe,jetreibubrega.LokalnoprimjenjenikvercetinsHXSili
PPDmoesmanjitipsorijaznelezijekoepotiskivanjembrojaupalnihstanicaukoi,inhibicijom
funkcionalne aktivnosti makrofaga i nastanka reaktivnih kisikovih spojeva (ROSa). Dodatno,
ukupni broj upalnih stanica u peritonealnoj upljini, indeks irenja makrofaga, lipidna
peroksidacija u jetri i bubregu, te enzimska aktivnost ALP i AST su smanjeni u mieva sa
psorijazomobraenimkvercetinom.Temeljemsvega,rezultatipodupiruinjenicudakvercetin,
kao snani antiupalni i antioksidativni spoj, moe biti djelotvoran u lijeenju upalnih bolesti
koe, bez toksinih posljedica. Termografija kao metoda moe biti primijenjena u praenju
upalnogprocesakoeupsorijazikaoiupraenjuuinkovitostikvercetinauobradipsorijaznih
lezija.
Kljunerijei:mijimodelpsorijaze,konelezije,kvercetin,termografija
N.Oroli,D.Odeh,M.Matakovi,V.Balta,M.Kunti,M.Kukolj,K.Sailo,D.iki
DivisionofAnimalPhysiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(nada.orsolic@biol.pmf.hr)
This study examined the possible positive effects of quercetin on animal model of psoriasis,
induced by the Hexyl salicylate or DinPropyl Disulfide irritant (PPD). Furthermore, we
evaluated the usefulness of thermography in psoriatic lesion regression. We monitored the
inflammation process by histopathological assessment of skin, thermographic scanning, total
number of inflammatory cells in peritoneal cavity, differential analysis of cells in peritoneal
cavity, macrophage spreading index, hematological and biochemical parameters, frequencies
of micronucleated reticulocytes, lipid peroxidation and glutathione assay in skin, liver and
kidney.TopicallyappliedquercetinwithHXSorPPDmayimprovepsoriaticlikeskinlesionsby
suppressingnumberofinflammatorycellsinskin,functionalactivityofmacrophages,andROS
production. In addition, total number of inflammatory cells in peritoneal cavity, macrophage
spreadingindex,lipidperoxidationinliverandkidney,andenzymaticactivityofALPandAST
werereducedinpsoriaticmicetreatedwithquercetin.Takentogether,theseresultssupport
thequercetinasanantiinflammatoryandantioxidativeagentandopenupnewpossibilitiesfor
its use in skin disorders without causing any toxic effect. Thermography as a method can be
applicable in examining of the inflammatory process in psoriasis and in evaluating the
effectivenessofquercetinandothertestedsubstances.
Keywords:mousemodelsofpsoriasis,skinlesions,quercetin,thermography
P133
NARINGENIN UBLAAVA PATOLOKE PROMJENE U JETRIMA I BUBREGU MIEVA S
DIJABETESOM
D.Sirovina1,N.Oroli2,M.ZovkoKoni3,G.Gregorovi1
1
Zoologijski zavod, Bioloki odsjek, Prirodoslovnomatematiki fakultet, Sveuilite u Zagrebu,
Zagreb,Hrvatska(sirovina@biol.pmf.hr)
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12.HBK27012 CBC
Zavodzaanimalnufiziologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuilite
uZagrebu,Zagreb,Hrvatska
3
Farmaceutskobiokemijskifakultet,SveuiliteuZagrebu,Zagreb,Hrvatska
Istraivanjeuinaknaringeninanarazinulipidneperoksidacijeihistopatolokepromjeneu
jetrima i bubrezima mieva s aloksanom izazvanim dijabetesom. Osim toga, istraeni su i
njegovi antioksidativni uinci in vitro (sposobnost vezanja slobodnih radikala, sposobnost
reduciranjaisposobnostkeliranjaFe2+iona).Dvadananakondavanjaaloksana(75mgkg1,
i.v.), mievi su, tijekom 7 dana, intreperitonealno (50 mg kg1 dnevno), primili alkoholnu
(0,5% v/v) otopinu naringenina. Iako je pokazao ogranieno antioksidativno djelovanje in
vitro, terapija alkoholnom otpopinom naringenina prouzroila je znaajno snienje razine
lipidne peroksidacije u jetrima i bubrezima kao i smanjenje stupnja vakuolizacije te broja
vakuoliziranih stanica jetara. Naznake popravka tkiva bubrega navode na zakljuak da se
blagotvoran uinak na bubrege moe postii terapijom kroz dulje vrijeme. Zatitni uinak
naringenina u razvoju komplikacija dijabetesa u mieva daje nadu da moe pokazati sline
uinkekodljudi.
Kljunerijei:dijabetes,naringenin,lipidnaperoksidacija,histopatologija
D.Sirovina1,NOroli2,MZovkoKoni3,GGregorovi1
1
DivisionofZoology,Departmentof Biology, FacultyofScience,UniversityofZagreb,Zagreb,
Croatia(sirovina@biol.pmf.hr)
2
DivisionofAnimalPhysiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia
3
FacultyofPharmacyandBiochemistry,UniversityofZagreb,Zagreb,Croatia
The effect of naringenin on lipid peroxidation and histopathological changes in liver and
kidneyofalloxaninduceddiabeticmicewereinvestigated.Inaddition,itsinvitroantioxidant
effects(DPPHfreeradicalscavengingactivity,reducingpowerandthecapabilitytochelate
Fe2+ ions) were assessed. Two days after alloxan injection (75 mg kg1, i.v.), naringenin
ethanolicsolution(0.5%v/v)wasgiventomiceintraperitoneally(50mgkg1perday)for7
days. Although it has shown limited antioxidant activity in vitro, naringenin administration
resultedinasignificantdecreaseoflipidperoxidationlevelinliverandkidneytissueaswell
asindecreasednumberofvacuolatedlivercellsanddegreeofvacuolization.Indicationsof
tissuerepairinkidneysuggestthatameliorationofdiabetesinducedrenaldamagecouldbe
achieved through a longer period of time. The protective effect of naringenin in the
development of diabetic complications in mice, gives a hope that it could show similar
effectsintreatmentofdiabetesinhumans.
Keywords:diabetes,naringenin,lipidperoxidation,histopathology
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12.HBK27112 CBC
GENETIKA,STANINAIMOLEKULARNABIOLOGIJA
GENETICS,CELLULARANDMOLECULARBIOLOGY
P134
PRIMJENAANALIZEMIKROSATELITNIHBILJEGANAPOPULACIJAMAVRSTAAstacusastacusI
AstacusleptodactylusUHRVATSKOJPRELIMINARNIREZULTATI
I.Maguire1,M.Jeli1,G.Klobuar1,A.tambuk1,M.Vuci1,F.Grandjean2
1
Sveuilite u Zagrebu, Prirodoslovnomatematiki fakultet, Bioloki odsjek, Zoologijski zavod,
Zagreb,Hrvatska(ivana.maguire@biol.pmf.hr)
2
University de Poitiers, UMR 7267 Ecologie et Biologie des Interactions, Poitiers Cedex,
Francuska
Kljunerijei:plemenitirak,uskokarirak,jezgrinibiljezi,unakrsnoumnaanje
I.Maguire1,M.Jeli1,G.Klobuar1,A.tambuk1,M.Vuci1,F.Grandjean2
1
UniversityofZagreb,FacultyofScience,DepartmentofBiology,DivisionofZoology,Zagreb,
Croatia(ivana.maguire@biol.pmf.hr)
2
UniversitydePoitiers,UMR7267EcologieetBiologiedesInteractions,PoitiersCedex,France
Recently,microsatellitemarkershavebeensuccessfullyappliedtorevealgeneticpatternsin
populations of crayfish species. In this research, we used four microsatellites primarily
aiming to check their possible usefulness in detecting hybrids between noble and narrow
clawed crayfish. These hybrids have been previously obtained under laboratory conditions,
andtheiroccurrenceinnaturalhabitats hasbeenmentioned in theliterature.Existenceof
possible hybrids between noble and narrowclawed crayfish in natural habitats was
approachedby morphometric analyses. Results on morphometry wereambiguous, and the
existenceofhybridshasnotbeenapprovedwithcertainty.Applicationofmicrosatellitesin
this research was done on the samples from pure A. leptodactylus and A. astacus
populations, from the mixed population, and on the samples of hybrids obtained from an
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experimental trial. Obtained results are complex, and their comprehensive interpretation
wouldrequireinclusionofmoreindividuals.Nevertheless,itwasprovedthatmicrosatellites
developed for A. astacus, and novel microsatellite marker isolated from A. leptodactylus,
gavesuccessfulamplificationforbothspecies.AnalysisofsamplesfromtheMrenicaRiver
(downstreamA.leptodactyluspopulation;upstreamA.astacuspopulation),gaveinsightinto
thepopulationstructureforbothspecies.A.astacusshowslowerdegreeofheterozygosity,
whatcouldbeaconsequenceofacrayfishplagueoutbreak.
Keywords:noblecrayfish,narrowclawedcrayfish,nuclearmarkers,crossamplification
P135
ISTRAIVANJEULOGEmikroRNAKODHERPESSIMPLEKSVIRUSA1
M.Matei1,S.Jonji2,I.Jurak3
1
SveuiliteuRijeci,Odjelzabiotehnologiju,Rijeka,Hrvatska(marina.matesic@biotech.uniri.hr)
2
Znanstvenicentarizvrsnostizavirusnuimunologijuicjepiva,MedicinskifakultetSveuilitau
Rijeci,Rijeka,Hrvatska
3
Zavodzahistologijuiembriologiju,MedicinskifakultetSveuilitauRijeci,Rijeka,Hrvatska
Herpessimpleksvirus1(HSV1)jehumanipatogenkojitijekomproduktivneilatentneinfekcije
eksprimira velik broj mikroRNA (miRNA), malih regulatornih RNA molekula. HSV1 miRH1 je
miRNAobilatoiiskljuivoeksprimiranatijekomproduktivneinfekcije.Zanimljivojedajedruga
miRNA,miRH6,kodirananaistomlokusualieksprimiranausuprotnomsmjeruiupotpunosti
komplementarna miRH1, obilato eksprimirana tijekom obje faze infekcije. Dosadanja
istraivanja dovela su do osnovne karakterizacije kinetike ekspresije ovih miRNA, meutim
njihovabiogenezaiulogaureplikacijivirusajenepoznata.TranskriptikojisuishoditemiRH1i
H6kaoiregulacijanjihovegenskeaktivnostinisupoznati,anaipreliminarnirezultatipomou
NorthernblotaukazujunarelativnokratketranskriptekojibimoglibitiishoditeovihmiRNA.
Detaljna karakterizacija det ektiranih transkripata je u tijeku. S druge strane, kako bismo
odredili ulogu miRH1 i H6, generirali smo mutantu sa delecijom u regiji koja kodira za ove
miRNA i trenutno smo u procesu generiranja povratnih mutacija. Preliminarna ispitivanja
pokazalasudadelecijskamutantanemaznaajnireplikacijskinedostatakinvitro,uodnosuna
divljitip,meutimkodeksperimenatainvivouoenajeslabijareplikacijamutiranogvirusa.Ovi
rezultatiukazujudamiRH1iH6imajuvanuuloguureplikacijiHSV1teedisekcijanjihovih
ulogabitidiskutiranaudetalje.
Kljunerijei:Herpessimpleksvirus1,mikroRNA,primarnitranskript
INVESTIGATINGTHEROLESOFmicroRNAsINHERPESSIMPLEXVIRUS1INFECTION
M.Matei1,S.Jonji2,I.Jurak3
1
University
of
Rijeka,
Department
of
biotechnology,
Rijeka,
Croatia
(marina.matesic@biotech.uniri.hr)
2
Center of Excellence for Viral Immunology and Vaccines, Faculty of Medicine, University of
Rijeka,Rijeka,Croatia
3
DepartmentofHistologyandEmbriology,FacultyofMedicine,UniversityofRijeka,Rijeka,Croatia
Herpessimplexvirus1(HSV1)isanimportanthumanpathogenthatduringproductiveandlatent
infectionexpressesalargenumberofmicroRNA(miRNA),smallregulatoryRNAmolecules.HSV1
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Keywords:Herpessimplexvirus1,microRNA,primarytranscript
P136
KARAKTERIZACIJAGENA/PROTEINAFAUIZMORSKESPUVESuberitesdomuncula
D.Perina1,M.Korolija2,M.PopoviHadija3,I.Grbea4,R.Belui3,M.Imeek1,C.Morrowe5,
T.BakranPetricioli6,A.Miko1,H.etkovi1
1
Zavodzamolekularnubiologiju,InstitutRuerBokovi,Zagreb,Hrvatska(dperina@irb.hr)
2
Centarzaforenzinaispitivanja,istraivanjaivjetaenja'IvanVueti'Zagreb,Hrvatska
3
Zavodzamolekularnumedicinu,InstitutRuerBokovi,Zagreb,Hrvatska
4
The Mina and Everard Goodman Faculty of Life Sciences, BarIlan University, RamatyGan,
Izrael
5
Queens University Belfast, Marine Laboratory, Portaferry, Northern Ireland, Ujedinjeno
Kraljevstvo
6
Zoologijskizavod,Biolokiodsjek,Prirodoslovnomatematikifakutet,SveuiliteuZagrebu,
Zagreb,Hrvatska
Gen FAU je smanjeno eksprimiran kod karcinoma prostate, dojke i jajnika. tovie, njegova
deregulacija je povezana s loom prognozom kod raka dojke. Spuve (Porifera) su ivotinje
bezpravogtkivakojesuseprveodvojileodzajednikogpretkasvihivotinja.Velikaveina
genapovezanasnastankomrakakodljudiimahomologeuspuvinomgenomu.Naastudija
pokazujedagenFAUizspuveSuberitesdomunculaodraavakarakteristikegenapretka,koji
seneznatnopromijeniotijekomevolucijeivotinja.FAUproteinizspuve,kaoinjegovljudski
homolog,poveavaapoptozuuljudskimstanicamaHEK293T.Toupuujedabiolokafunkcija
proteina FAU, obino povezana s "viim" ivotinjama, osobito u etiologiji raka, ima
biokemijskupodloguuspostavljenutijekomraneevolucijeMetazoa.Najvjerojatnijejepredak
svihivotinjaposjedovaoproteinFAUsastrukturomifunkcijamaslinimonimaurecentnim
verzijama proteina, ak i prije pojave pravih tkiva i nastanka tumora i metastaza. Nai
rezultati upuuju na mogunost koritenja bazalnih ivotinja kao jednostavnijeg modela za
prouavanjesloenihinterakcijauljudskojkancerogenezi.
Kljunerijei:snoRNA,FAU,RPS30,SNORA62,Porifera
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D.Perina1,M.Korolija2,M.PopoviHadija3,I.Grbea4,R.Belui3,M.Imeek1,C.Morrowe5,
T.BakranPetricioli6,A.Miko1,H.etkovi1
1
DivisionofMolecularBiology,RuerBokoviInstitute,Zagreb,Croatia
2
ForensicScienceCentre'IvanVueti',Zagreb,Croatia
3
DivisionofMolecularMedicine,RuerBokoviInstitute,Zagreb,Croatia
4
TheMinaandEverardGoodmanFacultyofLifeSciences,BarIlanUniversity,RamatyGan,Israel
5
QueensUniversityBelfast,MarineLaboratory,Portaferry,NorthernIreland,UK
6
DivisionofZoology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,Zagreb,
Croatia
FAU gene is downregulated in human prostate, breast and ovarian cancers. Moreover, its
dysregulation is associated with poor prognosis in breast cancer. Sponges (Porifera) are
animals without tissues which branched off first from the common ancestor of all
metazoans.Alargemajorityofgenesimplicatedinhumancancershavetheirhomologuesin
the sponge genome. Our study suggests that FAU gene from the sponge Suberites
domunculareflectscharacteristicsoftheFAUgenefromthemetazoanancestor,whichhave
changedonlyslightlyduringthecourseofanimalevolution.Wefoundproapoptoticactivity
of sponge FAU protein. Same as its human homologue, sponge FAU increases apoptosis in
humanHEK293Tcells.ThisindicatesthatbiologicalfunctionsofFAU,usuallyassociatedwith
"higher" metazoans, particularly in cancer etiology, possess biochemical background estab
lished early in metazoan evolution. The ancestor of all animals possibly possessed FAU
proteinwiththestructureandthefunctionsimilartoevolutionarilymorerecentversionsof
the protein, even before the appearance of true tissues and the origin of tumors and
metastasis. It provides an opportunity to use prebilaterian animals as a simpler model for
studyingcomplexinteractionsinhumancancerogenesis.
Keywords:snoRNA,FAU,RPS30,SNORA62,Porifera
P137
DISTRIBUCIJA PROTEINA UKLJUENIH U POLI(ADPRIBOZIL)ACIJU U SVIM DOMENAMA
IVOTA
D.Perina1,A.Miko1,J.Ahel1,H.etkovi1,R.aja2,I.Ahel3
1
Zavodzamolekularnubiologiju,InstitutRuerBokovi,Zagreb,Hrvatska(dperina@irb.hr)
2
Zavodzaistraivanjemoraiokolia,InstitutRuerBokovi,Zagreb,Hrvatska
3
SirWilliamDunnSchoolofPathology,UniversityofOxford,Oxford,UK
12.HBK27512 CBC
Kljunerijei:makrodomena,odgovornaoteenjeDNA,PARG,PARP,Poli(ADPriboza)
D.Perina1,A.Miko1,J.Ahel1,H.etkovi1,R.aja2,I.Ahel3
1
DivisionofMolecularBiology,RuerBokoviInstitute,Zagreb,Croatia(dperina@irb.hr)
2
DivisionforMarineandEnvironmentalResearch,RuerBokoviInstitute,Zagreb,Croatia
3
SirWilliamDunnSchoolofPathology,UniversityofOxford,Oxford,UK
Poly(ADPribosyl)ationisaposttranslationalmodificationofproteinsinvolvedinregulation
ofmanycellularpathways.Poly(ADPribose)(PAR)consistsofchainsofrepeatingADPribose
nucleotide units and is synthesized by the family of enzymes called poly(ADPribose)
polymerases (PARPs). This modification can be removed by the hydrolytic action of
poly(ADPribose) glycohydrolase (PARG) and ADPribosylhydrolase 3 (ARH3). Hydrolytic
activity of macrodomain proteins (MacroD1, MacroD2 and TARG1) is responsible for the
removalofterminalADPriboseunitandforcompletereversionofproteinADPribosylation.
Poly(ADPribosyl)ation is widely utilized in eukaryotes and PARPs are present in
representatives from all six major eukaryotic supergroups, with only a small number of
eukaryotic species that do not possess PARP genes. The last common ancestor of all
eukaryotes possessed at least five types of PARP proteins that include both mono and
poly(ADPribosyl)transferases.DistributionofPARGsstrictlyfollowsthedistributionofPARP
proteins in eukaryotic species. At least one of the macrodomain proteins that hydrolyse
terminal ADPribose is also always present. Therefore, we can presume that the last
common ancestor of all eukaryotes possessed a fully functional and reversible PAR
metabolism and that PAR signalling provided the conditions essential for survival of the
ancestraleukaryoteinitsancientenvironment.
Keywords:DNAdamageresponse,Macrodomain,PARG,PARP,Poly(ADPribose)
P138
VALPROAT NARUAVA RAST TAKORSKOG ZAMETKA I EKTOPLACENTALNOG STOCA EX
VIVO
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12.HBK27612 CBC
Na je cilj bio istraiti ex vivo utjecaj valproata, inhibitora histonske deacetilaze (HDI), na rast
zametka takora i ektoplacentalnog stoca. 9,5 dana stari zameci takora Fisher (tri zametna
listia)iektoplacentalnistocimikrokirurkisuizoliraniizdecidua.Zamecisukultiviraniredom14
dana,astocitridana,uEagleovomMEMuitakorskomserumu(1:1)nagranicitekuineizraka.
Valproat(2mMili1mM)itrihostatinAotopljenuDMSO(66nM)iDMSO(1,2%)koritenisuu
pokusima.Praenjeukupnirasteksplantatatakodasuvelikiimalipromjerimjerenisvakidrugi
dan,izraunatesuaree(A=1/2velikipromjerx1/2manjipromjerx)inormaliziranenapoetne
vrijednosti(A/A0).UstatistikojobradipodatakakoritenjeStudentovttest iliANOVA.Ukupni
rastzametakaiektoplacentalnihstoacakultiviranihsvalproatombiojeznaajnomanjiuodnosu
nakontrole,posebnopriveojkoncentracijivalproata(2mM).TrihostatinAtakoerjesmanjio
rastzametakauodnosunakontrolu(bezstatistikeznaajnosti),doksuuDMSOuzamecibilivei
negoutrihostatinuA,alimanjiodkontrolneskupine.Dobivenirezultatioutjecajuvalproatana
zametak i ektoplacentalni stoac, koji doprinosi razvoju posteljice, mogu biti vani za
razumijevanjeteratogenoguinkaantieptileptikavalproata.
Kljunerijei:valproat,takorskizametak,ektoplacentalnistoac,rast,exvivo
VALPROATE IMPAIRS GROWTH OF THE RAT EMBRYO AND THE ECTOPLACENTAL CONE EX
VIVO
Ouraimwastoinvestigateexvivoimpactofhistonedeacetylaseinhibitor(HDI)valproateon
growth of the rat embryo and the ectoplacental cone. 9.5dayold Fisher rat embryos (three
germ layers) and the ectoplacental cones were microsurgically isolated from deciduas. They
were separately cultivated for 14 days or three days, respectively, in Eagle's MEM and rat
serum(1:1)attheairliquidinterface.Valproate(2mMor1mM)ortrichostatinAdissolvedin
DMSO(66nM)orDMSO(1.2%)wereusedinexperiments.Tofollowoverallgrowth,majorand
minor diameters were measured every other day, areas were calculated (An= 1/2 major
diameter x 1/2 minor diameter x ) and normalized to the initial value (A/A0). Data were
statistically evaluated by Student's ttest or ANOVA. Overall growth of embryos and
ectoplacental cones cultivated with valproate was significantly impaired in comparison to
controls, being more impaired by higher valproate concentration (2 mM). Trichostatin A also
impaired growth of embryos in comparison to controls (no statistical significance) while in
DMSO they were constantly larger than with trichostatin A but smaller than controls. The
obtained results about valproate impact upon the embryo and ectoplacental cone
(contributingtoplacentaldevelopment)couldbeimportantforunderstandingtheteratogenic
effectoftheantiepilepticdrugvalproate.
Keywords:valproate,ratembryo,ectoplacentalcone,growth,exvivo
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P139
GENETSKA ANALIZA POPULACIJE ATLANTSKE PLAVOPERAJNE TUNE, Thunnus thynnus
(Linnaeus,1758)UKAVEZNOMUZGOJUPRIMJENOMMIKROSATELITNIHLOKUSA
I.Radoni1,.Trumbi2,T.egviBubi1,L.Grubii1,I.Mladineo1
1
Institutzaoceanografijuiribarstvo,Split,Hrvatska,(ivana.radonic.88@gmail.com)
2
SveuiliteuSplitu,Odjelzastudijemora,Split,Hrvatska
Atlantska plavoperajna tuna Thunnus thynnus (Linnaeus, 1758) je migratorna riba koja
nastanjuje Atlantski ocean i Sredozemno more. Od 1996. godine prakticira se njen kavezni
uzgoj u Hrvatskoj. Uzgoj tune donosi ekonomsku dobit, ali ulov, transport i adaptacija na
kaveze ribama su stresni to moe rezultirati padom imunosti i mortalitetima juvenilne ribe.
MikrosatelitisunajeioblikponavljajueDNAueukariotskomgenomu,lakoseamplificiraju
PCR reakcijom i predstavljaju odline genetike biljege u istraivanju populacijske strukture,
evolucije, identifikacije jedinki, odreivanju srodstva meu jedinkama te se esto koriste u
genetikim i medicinskim istraivanjima. Koristei mikrosatelitne motive identificirane na
sekvencamanormaliziranecDNAknjiniceplavoperajnetunekaoinicijalnogkalupa,razvilismo
multipleks PCR protokol s 15 lokusa smjetenih u blizini kodirajuih regija gena koji se
eksprimiraju prilikom stresa i imunosne reakcije. Na uzorcima DNA izoliranim iz fragmenta
peraja324tuneuzorkovanihprilikomulazapopulacijeuuzgoj,teprilikomizlovaistepopulacije
nakon1,5godinjeguzgojautvrenajerazlikauuestalostipolimorfizamaovihmikrosatelitnih
lokusa, to se moe dovesti u vezu s fitnessom jedinki u uzgoju. Istraivani lokusi ubudue
moguposluitikaobiljezizaotkrivanjejedinkiunutarpopulacijekojesuuslijedpolimorfizama
unutar imunosnih gena sklonije promjenama imunosnog sustava i imaju izraeniji rizik od
ugibanjakrozuzgojniciklus.
Kljunerijei:Atlantskaplavoperajnatuna,mikrosateliti,polimorfizam
GENETICANALYSISOFCAGEREAREDATLANTICBLUEFINTUNA,Thunnusthynnus(Linnaeus,
1758)POPULATIONBYAPPLICATIONOFMICROSATELLITELOCI
I.Radoni1,.Trumbi2,T.egviBubi1,L.Grubii1,I.Mladineo1
1
InstituteofOceanographyandFisheries,Split,Croatia(ivana.radonic.88@gmail.com)
2
UniversityofSplit,UniversityDepartmentofMarineStudies,Split,Croatia
Atlantic bluefin tuna Thunnus thynnus (Linnaeus, 1758) is a migratory fish that inhabits the
AtlanticOceanandtheMediterraneanSea.Since1996s,Croatiapracticescagebreedingofthis
species.Tunafarmingbringseconomicbenefits,butthecatch,transportationandadaptationto
the cages are stressful and might result in decreased immunity and mortalities in fish.
MicrosatellitesarethemostcommonformofrepetitiveDNAineukaryoticgenomes,theycanbe
easilyamplifiedbythePCRreactionandtheyrepresentexcellentgeneticmarkersinthestudyof
populationstructure,evolution,identificationofindividuals,determinationofkinshipsbetween
individuals and they are widely used in various fields of genetics and medical research. Using
microsatellitemotifsidentifiedinthesequencesofanormalizedcDNAlibraryoftunausedasthe
initial template, we developed a multiplex PCR with 15 loci located near the coding region of
genesexpressedduringstressandimmunereaction.DNAwasisolatedfromfinclipof324tuna
individuals at the moment of entrance in the cages and at harvest after 1.5 years of rearing.
Differencesinthefrequencyofpolymorphismsofthesemicrosatelliteloci,whichcanberelated
toindividualfitnessoffishthroughthebreedingcycleweredetected.Thoselociinthefuturemay
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beusefulasmarkersfordetectingindividualswithinthepopulationwhich,duetopolymorphism
withintargetgenes,haveahigherriskofdeaththroughthebreedingcycle.
Keywords:Atlanticbluefintuna,microsatellites,polymorphism
P140
EKSPRESIJA I IDENTIFIKACIJA NOVIH PROTEINA DJELOMINO NEUREENE TERCIJARNE
STRUKTURE (IDP) IZ BILJKE URONJAK (Arabidopsis thaliana) NAKON IZLAGANJA
ABIOTIKOMSTRESU
I.Tomljanovi,K.Skube,D.Pavokovi
Zavodzamolekularnubiologiju,Biolokiodsjek,Prirodoslovnomatematikifakultet,Sveuilite
uZagrebu,Zagreb,Hrvatska(dubravko@biol.pmf.hr)
Nativno neureeni proteini (engl. intrinsically disordered proteins, IDP) pripadaju nedavno
priznatojskupiniproteinakojisubiolokiaktivniusprkosnedostatkujasnodefiniranetercijarne
strukture. Takvi proteini esto su adapterske ili signalne molekule i aperoni. Smatra se da
imajuvanuuloguuodgovorubiljakanastres,nootomenemamnogopodataka.Odvelikeje
vanostirazjasnitimolekularnemehanizmeodgovoranastresjeronznaajnoograniavarasti
prinosindustrijskihbiljaka.CiljovogistraivanjabiojeanaliziratiekspresijuiidentificiratiIDPu
biljciuronjakuArabidopsisthaliananakonizlaganjahladnoi.Globularniproteinieliminiranisu
zagrijavanjem uzoraka na 99 C. IDP i ostali globularni proteini razdvojeni su nativnom i
denaturirajuom 2D PAGE s 8M urejom. U biljaka tretiranih hladnoom, denzitometrijska
analizaukazalajenaizostanakekspresije10proteina,smanjenjeekspresijejednogipoveanje
ekspresijeetiriproteinauodnosunakontrolu.Masenomspektrometrijomidentificiranoje29
razliitih proteina unutar 40 proteinskih mrlja. Bioinformatikim alatom PrDOS za procjenu
neureenosti proteina na osnovi analiza primarne strukture otkriveno je 13 novih IDP.
AnalizompremarjenikuGeneOntologypokazanojedaveinaproteinasudjelujeuabiotikom
stresu vezivanjem razliitih biolokih molekula, kao to su DNA, RNA i proteini. Kao prvo
istraivanjeIDPuproteomubiljnogorganizmaovajradjeznaajanzaitavopodrujeIDP.
Kljunerijei:adapterskemolekule,aperoni,procjeniteljineureenosti,streshladnoa
I.Tomljanovi,K.Skube,D.Pavokovi
DivisionofMolecularBiology,DepartmentofBiology,FacultyofScience,UniversityofZagreb,
Zagreb,Croatia(dubravko@biol.pmf.hr)
Intrinsicallydisorderedproteinsandproteinregions(IDP/IDR)arearecentlyrecognizedgroup
ofproteinswhicharebiologicallyactivedespitetheirlackofawelldefinedthreedimensional
structure.IDPsareoftenchaperones,adapterorsignalingmolecules.Theyarethoughttohave
importantrolesinabioticstressresponse,butsupportingdataisstillscarce.Itisimportantto
clarifythemolecularmechanismsofabioticstress responsebecausestresscanseverelylimit
industrial plant growth and yield. The aim of this study was expression analysis and IDP
identification in the model plant Arabidopsis thaliana during cold stress. Globular proteins
were eliminated by heating extracts at 99 C. IDPs and remaining globular proteins were
separated using a native/8M urea 2D polyacrylamide gel electrophoresis. Densitometric
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analysis indicated that, when compared to the control group, coldtreated plants lacked
expression of ten proteins, while four were upregulated and one was downregulated. Mass
spectrometry identified 29 different proteins from a total of 40 excised spots. 13 novel IDPs
werediscoveredusingPrDOS,asequencebasedbioinformaticdisorderpredictiontool.Gene
Ontology analyses indicated that the majority of the proteins were involved in abiotic stress
throughbindingofdifferentligands,suchasDNA,RNAandproteins.AsthefirststudyofIDPs
inaplantproteome,ourresearchwillhaveasignificantimpactonthewholeIDPfield.
Keywords:adaptermolecules,chaperones,disorderpredictors,disorderpredictionsoftware,
coldstress
P141
GENOMIKOLJKAAUKAZUJUNATRANSPOZICIJSKUMOBILNOSTSATELITNIHPONAVLJANJA
T.VojvodaZeljko,E.atovi,M.Plohl
InstitutRuerBokovi,Zagreb,Hrvatska(tanja.vojvoda@irb.hr)
Satelitna DNA (satDNA) i transponirajui elementi (TE) ine dvije najznaajnije grupe
ponovljene DNA u eukariotskim genomima. S obzirom na nedostatak informacija o DNA
sekvencamakojeokruujunizovesatDNA,najeciljbioistraitisekvencevezanezasatelitna
ponavljanjakoristeisekoljkaimakaomodelnimorganizmima.Asembliranigenomkamenice
Crassostrea gigas (Zhang et al. 2012) pretraen je na sekvence koje okruuju njezinu satDNA
Cg170 (Clabby et al. 1996). Takoer, u tri druge vrste koljkaa (Ruditapes decussatus, R.
philippinarumiDonaxtrunculus)eksperimentalnimpristupomdobivenesusekvencenekoliko
ranije opisanih satDNA i njihovih okruenja. Nekoliko fragmenata je nasumino odabrano i
detaljno opisano. Dobiveni rezultati pokazuju da se TEsline sekvence (naroito DNA
transpozoni) nalaze u blizini satDNA te ukazuju da je intenzivno irenje satDNA sekvenci u
genomu vjerojatno uzrokovano procesima koji su vezani za transpoziciju. Navedeno promie
satDNA u vrlo dinamine sekvence koje bi mogle predstavljati graevne jedinice genoma na
slian nain te usporedivog znaaja kao to su to TE. Stoga, iako se tradicionalno smatraju
dvjemaodvojenimgrupama,satDNAiTEsevrlovjerojatnointimnoisprepliuuprocesuirenja
pogenomu,mnogovienegotosetodosadsmatralo.
BIVALVEGENOMESSUGGESTTRANSPOSITIONDRIVENMOBILITYOFSATELLITEDNAREPEATS
T.VojvodaZeljko,E.atovi,M.Plohl
RuerBokoviInstitute,Zagreb,Croatia(tanja.vojvoda@irb.hr)
Satellite DNAs (satDNAs) and transposable elements (TEs) are two most abundant classes of
repetitiveDNAsfoundineukaryoticgenomes.SinceknowledgeregardingDNAsequencesthat
surround satDNA repeats in the genome is mostly missing, our aim was to explore satDNA
associated sequences using bivalve genomes as a model system. Here, we searched the
genome assembly of the oyster Crassostrea gigas (Zhang et al. 2012) to explore the
surroundings of its satDNA Cg170 (Clabby et al. 1996). In addition, surroundings of several
satDNAsequencesalreadycharacterizedinthreeotherbivalvespecies(Ruditapesdecussatus,
R.philippinarumandDonaxtrunculus)weregainedexperimentally.Foradetailedstudy,some
fragments were randomly selected and annotated. Obtained results revealed TElike
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sequences, mostly DNA transposons, in the closest vicinity of satDNAs and indicate that
intensive shuffling of satDNA sequences in the genome is predominantly driven by a
transpositionrelated process. These findings promote satDNAs as highly dynamic sequences
thatmightrepresentgenomebuildersinasimilarwayandwithacomparablerelevanceasTEs.
Thus, although traditionally considered as two distinct classes, satDNAs and TEs seem to be
much more intimately interlinked in the process of spreading throughout the genome than
previouslythought.
Keywords:satelliteDNA,transposableelements,tandemrepeatflankings,bivalves
P142
RAZLIKE U OTPORNOSTI NA ANTROPOGENI STRES IZMEU INVAZIVNE I NATIVNE VRSTE
KOLJKAA
I.Bonjak1*,A.Bielen1,M.Jakli2,M.Cvitani3,K.Sepi4,T.Simi2,J.Lajtner3,S.Hudina3
1
SveuiliteuZagrebu,Prehrambenobiotehnolokifakultet,Zavodzabiokemijskoinenjerstvo,
Zagreb, Hrvatska; *trenutno zaposlena u: Sveuilite u Zagrebu, Prirodoslovnomatematiki
fakultet,Botanikizavod,Zagreb,Hrvatska(ibos254@gmail.com)
2
NacionalniInstitutzaBiologiju,Ljubljana,Slovenija
3
SveuiliteuZagrebu,Prirodoslovnomatematikifakultet,Zoologijskizavod,Zagreb,Hrvatska
4
SveuiliteuLjubljani,Biotehnikifakultet,Biolokiodsjek,Ljubljana,Slovenija
Otpornost na promjene u okoliu esto se navodi kao jedan od kljunih imbenika uspjeha
invazivnih stranih vrsta. No, empirijski dokazi za pretpostavku kako invazivne vrste bolje od
autohtonih podnose nepovoljne uvjete u okoliu su malobrojni. U ovom smo istraivanju
usporedili otpornost na toplinski stres i oneienje cinkom (ZnCl2) kod dvije filogenetski
srodnevrsteslatkovodnihkoljkaa,autohtonevrsteAnodontaanatinaiinvazivnestranevrste
Sinanodonta woodiana. Procijenili smo njihov odgovor na stres koristei nekoliko testova
staninog odgovora: stopu metabolizma (sustav prijenosa elektrona, eng. electron transport
system ETS), aktivnost mehanizma multiksenobiotike otpornosti (eng. multixenobiotic
resistanceMXR),ienzimatskebiomarkerestresakolinesterazu(ChE),glutationStransferazu
(GST)ikatalazu(CAT).Sveukupno,invazivnaS.woodianajemnogoboljepodnosilanepovoljne
uvjete.Ovojebilovidljivoiz(i)znaajnoveeaktivnostiMXRmehanizma;(ii)znaajnovieETS
aktivnosti, i (iii) nieg odgovora enzimatskih biomarkera stresa u uvjetima toplinskog stresa i
oneienja cinkom. Vea otpornost na toplinski stres je vrlo vana fizioloka osobina za
invazivniuspjehvrsteS.woodiana,posebiceukontekstuklimatskihpromjena.
Kljunerijei:invanzivniuspjeh,toplinskistres,oneienjecinkom,slatkovodnikoljkai,stopa
metabolizma,aktivnostMXRmehanizma
DIFFERENCESINTOLERANCETOANTHROPOGENICSTRESSBETWEENINVASIVEANDNATIVE
BIVALVES
I.Bonjak1*,A.Bielen1,M.Jakli2,M.Cvitani3,K.Sepi4,T.Simi2,J.Lajtner3,S.Hudina3
1
University of Zagreb, Faculty of Food Technology and Biotechnology, Department of
BiochemicalEngineering,Zagreb,Croatia,*currentlyat:UniversityofZagreb,FacultyofScience,
DepartmentofBotany,Zagreb,Croatia(ibos254@gmail.com)
2
NationalInstituteofBiology,Ljubljana,Slovenia
3
UniversityofZagreb,FacultyofScience,DepartmentofZoology,Zagreb,Croatia
4
UniversityofLjubljana,Biotehnicalfaculty,DepartmentofBiology,Ljubljana,Slovenia
th
12.HBK28112 CBC
Tolerance towards environmental stress has been frequently considered as one of the key
determinantsofinvasionsuccess.However,empiricalevidencesupportingtheassumptionthat
invasivespeciesendurebetterunfavourableconditionscomparedtonativespeciesislimited
andhasevenyieldedopposingresults.Weexaminedthetolerancetothermalstressandheavy
metalzincpollution(ZnCl2)intwophylogeneticallyrelatedandfunctionallysimilarfreshwater
bivalve species, native Anodonta anatina and invasive Sinanodonta woodiana. We assessed
theirresponsetostressusingseveralcellularresponseassays:metabolicrates(ETSelectron
transportsystem),efficiencyofthemultixenobioticresistance(MXR)mechanismactivity,and
enzymatic biomarkers (ChE cholinesterase, GST glutathioneStransferase and CAT
catalase).Overall,invasiveS.woodianacopedmuchbetterwithunfavourableconditions.This
was evident from (i) significantly more pronounced MXR mechanism activity; (ii) significantly
higherETSactivity,and(iii)lowerresponseofstressrelatedenzymes(ChE,GSTandCAT)under
thermal stress and ZnCl2 pollution. The overall better tolerance to thermal extremes is an
especially important physiological benefit for further invasion success of S. woodiana in
Europeanfreshwaters,especiallyinthecontextofclimatechange.
Key words: invasion success, thermal stress, heavy metal zinc pollution, freshwater mussels,
metabolicrate,MXRmechanismactivity
P143
ISPITIVANJEULOGESTANINIHmiRNAUREPLIKACIJIHERPESSIMPLEKSVIRUSA1
A.Zubkovi1,2,I.Ratkaj1,2,I.Jurak1,2
1
SveuiliteuRijeci,Odjelzabiotehnologiju,Rijeka,Hrvatska(azubkovic@uniri.hr)
2
Znanstvenicentarizvrsnostizavirusnuimunologijuicjepiva,MedicinskifakultetSveuilitau
Rijeci,Rijeka,Hrvatska
Tijekominfekcijevirusiznaajnomijenjajubiologijustanicetepritompodreujuiusmjeravaju
njezine funkcije u smjeru efikasne replikacije. Tako je nedavno pokazano da virusna infekcija
dovodiidoznaajnihpromjenauekspresijimiRNAdomaina,azatosesmatradabimoglo
dovoditi do stvaranja pogodnih uvjeta za replikaciju virusa. Promjene u ekspresiji staninih
miRNAkodinfekcijeherpessimpleksvirusa1(HSV1)uoenesuunekolikostudija,meutim
istraivanjadisregulacijemiRNAdomainateulogatakvihpromjenanisudetaljnoanalizirane.
Naagrupaje,koristeisemasivnimparalelnimsekvenciranjeuzorakaproduktivneilatentne
infekcije HSV1, uoila znaajan porast ekspresije odreenih staninih miRNA u svim
analiziranim uzorcima. Porast ekspresije miRNA dodatno smo potvrdili pomou kvantitativne
lananereakcijepolimerazomurealnomvremenu(qRTPCR).Ovirezultatimoglibiukazivatina
to da HSV1 ima sposobnost indukcije ekspresije miRNA radi postizanja optimalnih uvjeta za
replikaciju, ili alternativno, poveanje ekspresije miRNA domaina moglo bi predstavljati
obrambeni odgovor stanice na infekciju. Kako bi odgovorili na ova pitanja generirali smo
vektore za transdukciju stanica kojima emo poveati ekspresiju miRNA od interesa, te smo
trenutnouprocesugeneriranjastabilnotransduciranihstaninihlinijanakojimaemotestirati
virusnureplikaciju.Metodolokipristupitehnikidetaljistudijebitieprikazaniuovomradu.
INVESTIGATINGTHEROLESOFHOSTmicroRNAsINHERPESSIMPLEXVIRUS1INFECTION
A.Zubkovi1,2,I.Ratkaj1,2,I.Jurak1,2
1
UniversityofRijeka,Departmentofbiotechnology,Rijeka,Croatia(azubkovic@uniri.hr)
th
12.HBK28212 CBC
Center of Excellence for Viral Immunology and Vaccines, Faculty of Medicine, University of
Rijeka,Rijeka,Croatia
Viruses significantly perturb cell metabolism during infection enabling an efficient virus
replication.Recently,ithasbeenshownthatvirusinfectionleadsalsotosignificantchangesin
the expression of cellular miRNAs, which in turn might result in a favorable environment for
the virus. Similar to other viruses studied thus far, herpes simplex virus 1 (HSV1) induces
differential expression of cellular miRNA, however the exact role of such up or down
regulationhasnotbeenelucidated.Usingmassiveparallelsequencing,ourgrouphasobserved
asignificantincreaseintheexpressionofcertaincellularmiRNAinseveraldifferentcelllines
and cells latently infected with the virus. Moreover, we confirmed the increase in the
expression of host miRNAs by quantitative real time polymerase chain reaction (qRTPCR).
TheseresultsindicatethatHSV1hastheabilitytoinduceexpressionofthemiRNAstoachieve
optimalconditionsforefficientreplication;oralternatively,theincreaseinexpressionofhost
miRNAs may represent a defense response to the infection. To answer these questions, we
generated a set of vectors for transduction of cells to elevate the expression of miRNAs of
interest,andwearecurrentlyintheprocessofgeneratingstablytransducedcelllinestotest
viral replication. The methodological approach and the technical details of the study will be
presentedinthispaper.
th
12.HBK28312 CBC
KAZALOAUTORA
INDEXOFAUTHORS
A
AcmanM.
AdroviA.
AhelI.
AhelJ.
AjtiR.
AladroviJ.
AljaniG.
AmouretJ.
AndriM.
AniiI.
AnkonP.
AntoliB.
AntoloviJ.
AntonelliF.
AntoniO.
AntunoviDuniJ.
ArntzenJ.W.
ArslanP.
Avdakovi A.
O120
O9
P137
P137
O64
O125
O72
O37
O86
O101
P68
O77,O82
P62
O12
O7,O11
O90
O69
P113
O3
B
BabiD.
BabiS.
BabiM.
BajdB.
BakariR.
BakoviV.
BakranPetricioliT.
BalenB.
BalkoviI.
BaltaV.
BaranovV.
BarieviD.
Barievi A.
BarieviM.
BariB.
BariiJ.
BarZviD.
BastiM.
BaiPalkoviP.
BakieraS.
BatelR.
BatrRusuD.
BatistiM.
BauerN.
BegiV.
BegoviL.
BelaniA.
P4
P109,P110
P79,P84
O18
PL2
O41,O42,P30
P136
O94,P87
P93
P122,P132
O104
O44
O81,O112
P3
O13
P109,P110
PL5,O94
O34
O119
O51,P38,P39
O81
O44
P63
O89,P80
O34
O92,P11
O23
BeluiR.
BeninaB.
BeniP.
BenkoviV.
BesendorferV.
BielenA.
BilanM.
BiliJ.
BiliDujmuiN.
BiluiT.
BistroviM.
BjedovD.
BlaeviM.
BlaeviS.
BlaeviS.A.
BobanJ.
BokorL.
BogdanM.
BogdanoviT.
BogutI.
BokanI.
BoriI.
BosakS.
BonjakI.
BourdineaudJ.P.
BraiM.
BrajiJuricaD.
BriKaraonjiI.
BrthesD.
BrischouxF.
BrmeM.
BruvoMaariB.
BudimirA.
BugnyarT.
BujI.
BukovacA.
BuliM.
BuliJakuF.
BundoneL.
BuriI.
P136
P9
P10
P112,P127
O46
P4,P142
O83
O93
P31
O93
O96,P88
P10
P10,P98
P13,P14
P123
P52,P64
O46
O71
O95
P27
P108
P1
O76,O87,P65
O76,O87,
P4,P142
O111
O107
P70
P112
O111
P47
O97
IO1,P33
O97
O124
O36,O60,P18
O3
O21
P138
P62
O73
C
CaladoR.
Car A.
CaroviStankoK.
CesarV.
O83
P67,P95
O44
O90,O91,
O92,O123
th
12.HBK28512 CBC
CesarecR.
CetiniI.
CetiniK.
ChatzopoulouP.
CholevaL.
CindriM.
CipotM.
CizeljI.
CogalniceanuD.
CoppolaE.
CorbinJ.
CrnanP.
CrnecD.
CurmanM.
CvitaniM.
CvitkoviI.
CvjetkoP.
CzvekL.
avloviK.
engiM.
erbaD.
erniS.
izmekH.
oliI.
oRakovacR.
rgarB.
ukoviT.
upiI.
aletaM.
O58,O59
O87
P68
O44
O60
O25
P40
O13,O66,
P42,P50
O71
P62
P77
P17
O25,O32
P79,P84
P142
O82,P66,P75
P87
P94
O60
P61
P93
O116
P14
O102,P129
P109,P110
O105
P19
P89
etkoviH.
osiM.
uri A.
urkoviPericaM.
D
DajiStevanoviZ.
DamjanoviM.
DeTrochM.
DekaniS.
DekiR.
DelaI.
DennisS.
DereaninL.
O9,O36,
P18,P101
P136,P137
P86
O59,P41
O117,O118
O44
P99
P69
P20
P51
O125
O41,O42
P20
DesnicaS.
DespalatoviM.
DvaiGy.
DigulaO.
DionsioG.
DjurakicM.
DobriniP.
DobroviA.
DomazetLooT.
DomazetoviZ.
DoriV.
DragieviB.
DragiRunjakT.
DrakiM.
DrakuliS.
DrakuloviD.
DrainaT.
Drdar.
DrobneD.
DudaS.
DuliJ.
DumitrescuA.
DmmerI.M.
DupliA.
DyugmedzievA.
D
DukiG.
aniA.
ikiD.
oreviS.
E
ErdelezA.
ErgoviV.
ErkmenB.
ErkoF.
EtemS.
F
FabacS.
FabijaniV.
FantinaD.
O4,P7
O82,P66,P75
P94
O22
O83
O48,O49
O120
P17
PL2
P9
P17
O74
O20
P13,P14
P42
O75
O105
P9
PL7
O93
O74
O71
O54,O55
O9,O10,
O13,P16,
P19
P43
O64
P2
O61,O102,
O114,P124,
P128,P132
O64,P55
O40
P93
O109
O109,P111
P48
P21
P21
P3
th
12.HBK28612 CBC
FaragunaF.
FeaG.
FeiglV.
FeldhaarH.
FeljaA.
FerriJ.
FinderleT.
FortunaC.
FortunaC.M.
FraniI.
FraniZ.
FranjeviD.
FreyhofJ.
FriiJ.
FuchsR.
FurdekM.
FutoM.
GrgiM.
GrgurevM.
GrguriZ.
GrigorszkyI.
GroziD.
GrubiZ.
GrubiiL.
GubiM.
GlA.
GlG.
GunholddeOliveiraT.
GusheskiD.
GuyonnetD.
GuvicaG.
H
HackenbergerK.B.
HackenbergerK.D.
G
GabriI.
GangaiB.
GaraiD.
GariR.
GaoSokaD.
GhiaD.
GlamuzinaB.
GligoraUdoviM.
GodrijanJ.
GoluboviA.
GoluaE.
GombocS.
GompertZ.
GonceariucM.
Goriki.
GottsteinS.
Gottstein.
GovediM.
GraanR.
GrandjeanF.
GraovacN.
GrbacI.
GrbeaI.
GrdiaM.
GregoroviG
GreguraD.
GrgacR.
P34
O37
O110
P42
P10
P70
P98
P74
P71
O14,P12
P124
O37,O46,
O47,
O61,P34
O9
P51
P124
O40
O37,P45
P5
O78
O19
P63
O123
O37
O15
P94,P108
O76
O67
P128
O98
O41,O42
O44
O72
P108
P50
O37,P32
O85
O37,P32,
P134
O29
IO1
P136
O44
P133
O44
P11
HafnerD.
HalapirFrankoviR.
HalpernB.
Hamer B.
HamidoviD.
HandschuhS.
HaxhiuI.
HayretdaS.
HelferV.
HernandezMilanG.
HerrelA.
HlavatiD.
HolcerD.
HorvatKneeviA.
HorvatekD.
HorvatiJ.
HraniloviD.
Hraovec B.
HrenoviJ.
HrgaI.
Huber.
HudinaS.
I
IbraliuA.
IdojtiM.
IgnatovaIvanovaT.
O95
O3,O98
P5
P94
O102
O122
P139
P3
P111
P111,P113
O124
P104
P32
P4,P5
IO4,O11,
O108, O110,
P8,P31,P35
O5,O110,
P35
P67,P95
O27,P28
P45
O81,O112
O4,O17,O107,
P123
P53
O70
O109
O68
P62
PL6,O48
P44
O80,P71
P127
P50
P15
P123
O9
P85
O8,P118
O125,P7
P6,P142
O44
O117
P48
th
12.HBK28712 CBC
IkoviV.
IlieskaR.
IlijaI.
ImeekM.
ImpetuosoA.
IvanchevI.
IvaniI.
IvanekI.
IvaniK.Z.
IvankoviS.
IvanoviA.
IvkoviM.
J
JchM.A.
JakliM.
JaklinA.
JambroviM.
JanevHutinecB.
JantolN.
JaspricaN.
JeliD.
O57
P104
P16
P136
P74
P48
O78
O106,P99
O2
P85
O69,P57
O103,O104
P33
P142
O81,O84
P8
O13
O43
P67,P95
O13,O51,
O54,O55,
O56,O59,
O60,O61,
O62,O63,
O66,O68,
P38,P41,
P45,P49,
P50,P54,
P58,P61
O16,P16,
P96
O37,O61,
O63,P6,P32,
P45,P96,
P134
O12
O16,P7
O117,O118
P135
O108,P54
O64
P33
O123
O18
O44
P135,P143
O89
JeliK.
JeliM.
JeranN.
JeremiJ.
JeiM.
JonjiS.
JovanoviO.
JoviD.
JovoviL.
JoziI.
JugHartmanM.
JugDujakoviM.
JurakI.
JuraniM.
JuranoviCindriI.
JuricaK.
JuriLekiG.
K
Kadi.
KajanK.
KalafatiM.
KalamujiB.
KaleziM.L.
KamberoviJ.
KapeljS.
KapetanoviT.
KaraicaD.
KarasuBenliA..
KarinV.
KarliR.
KaubaV.
KataniZ.
KatanoviI.
KatuiBojanacA.
KatuiL.
KayiranTuncaS.
KekiT.
KepecS.
KerovecM.
KettG.
KeviN.
KirinT.
KiNovakD.
KlasiM.
KletekiE.
KletekiN.
KlobuarA.
KlobuarG.
KlobuarK.
KljajiK.
KoiA.
KohM.
KoletiN.
KollerK.
KonradKovaP.
P83
P112
P138
O28
P10
P34
O9
O64
P108
P2
P67,P95
O60,O62
P111,P113
O117
IO5
P114
O118
P96,P99
P138
O1
P113
O43
P110
P97
O80
O47,P34
P1
P97
O119,O120
O53,P81
O21,P22,
P26,P81
O100
O37,O40,
O41,O42,
O61,O63,
O111,P32,
P109,P110,
P117,P134
O125
P11
P15
P93
O47,P20,
P100
P46
P46
th
12.HBK28812 CBC
KooistraW.
KopjarN.
KoraP.
KorenA.
KorenT.
KorleviP.
KornilevY.
KorolijaM.
KostadinoviH.
KovaM.
KovaevA.
KovaeviG.
KovaeviJ.
KraljBorojeviK.
KrasiJ.
KrasniqiE.
KriviG.
KrivohlavekA.
KrizmaniI.
KrianJ.
KrstevskaK.
KrstinLj.
KrstiniP.
KrstuloviN.
KruiP.
KuiniM.
P106
KukavicaJovanoviB.
KukoljM.
KuntiM.
KuntnerM.
KurtekI.
KurtoviA.
KuG.
KusakJ.
KutleaP.
KutluH.M.
KvarantanM.
P65
P112,P114
O119,O120
IO5
O98,O99,
P91,P98
O61
P47,P48
P136
P24
O107
P68
P34
O90
P100,P108
O114
O44
O125
P118
O64
P2
P104
O118
O17
P121
O75,O86,
O87,O88,
P68
P33,P98,
P51
P122,P125,
P126,P128,
P129,P130,
P132
P122,P125,
P126,P128,
P129,P130,
P131,P132
O72
O96
O123
P115,P119
O125,P7
O10,O13
P115,P119
O86
LabakI.
LabaHorvatM.
LabusN.
LackoviG.
LadavacLj.
LadiA.M.
LajtnerJ.
LoniV.
LovreniL.
LuciA.
LuiD.
LuiP.
LugarL.
LuiselliL.
LukaM.
LukanovS.
LukiI.
LukiN.
Luka.
LunkoP.
P23
O26,P25
O64
O85
P98
P120
O39,O105,
P142
O90,O92
P124
O94
P89
P23
PL8
O99,P39,
P49,P58
IO5
O85
O53
P61
O89,P80
O91,O123
P93
P40
O44,O45
P83
O17
P49
O106,O107,
P42,P99
O11,O108,
O110,P8
O86
O39
P6
O78,O79
O75
O35
O64
IO2,O66,
P50
P53
O14
P51
O19,O20
P19
LaliA.
LandekaI.
LandekaK.
LandekaN.
LatinK.
LaucG.
LauB.
LawrenceM.S.
LazarB.
LeinerS.
LeloS.
LeljakLevaniD.
LepeduH.
LesjakM.
LenikA.
LiberZ.
LikiS.
LindiV.
LisiarP.
LisiiD.
O76,O87
Lonari.
LJ
LjubeiZ.
th
12.HBK28912 CBC
LjubisavljeviK.
O57,O64,
P45,P57
O106,O107,
P99
LjutinaM.
M
MacanJ.
MackelworthP.
MaguireI.
MajcenP.
MajiB.
MajsecK.
MaksimoviV.
MaksimoviZ.
MalariK.
MaleeviN.
MandiT.
MariZ.
MarinkoI.
MariM.
MarinF.
MarkiJ.
MartelA.
MartinkoviF.
MaslaA.
MaslaM.
MassenJ.J.M.
MatakoviM.
MatasV.
MateiM.
MatiSkokoS.
MatonikinKepijaR.
MatoeviD.
MatoiB.
MatuliD.
MayerW.
MazijaM.
MedakoviD.
MediN.
MejdandiM.
MekiniS.
MerdiE.
MerklerM.
MesariG.
O113
O80
O37,P6,P32,
P134
P128
P83
P82
P86
P56
O111
P20,P100
O102
O9,O36,
P101
O123
P29,P69
O84
O93
P56
P50
P116
O3,O7,P116
O124
P122,P132
P68
P135
P70,P124
O103
O14
P13
O101
IO1
P9
O84
O112
O76,O87,
P100
P52
O29,O96,
O100,P23,
P88,P92
P123
P98
MesariM.
MesiZ.
MietiStankoviV.
MihaliM.
MihaljeviLj.
MihaljeviM.
MihaljeviS.
MihaljeviZ.
MihaljeviZ.
MihanoviH.
MihiI.
MihinjaT.
MihociI.
MikacI.
MikinacN.
MikoA.
MikuliS.
MikukaA.
MilankovV.
MiliA.
MiliM.
MiliaM.
MilojkoviOpsenicaD.
MiloslaviM.
MiloeviD.
Miloevi.
MioM.
MioiStoiJ.
MiiD.
MitiB.
MladineoI.
MladiniM.
MlinarecJ.
MlinariS.
MoibobM.
MojsoviuiA.
MorajN.
MorroweC.
MotahS.
MrakoviM.
MrdakD.
MriG.
MllerM.
MullerW.E.G.
MurtiS.
O25
O7,P2
O60,P33,
P106
P24
O90
P105
P80
O103
O104
O87
P11
O36
P98
P108
O125
P136,P137
P25
P10,P11
O49
P105
P114
O102,O103,
O104
P112
O78,O79
O9
P93
P42
O80,P71,P76
P86
O8,P37
P139
P114
O46
O91,O92
O89
O22,P3,
P120
P68
P136
O86
O9,O36,
P18,P101
O9
P108
O68
O81
O44
th
12.HBK29012 CBC
MustaA.
MustaB.
MustafiP.
N
NagyS.A.
NanjappaD.
NpruAljaniM.
NatchevN.
NaumovB.
NemetM.
NemravaJ.
NitM.
NosilP.
NovakAgbabaS.
NovoselM.
NovoseliD.
NuskernL.
NJ
NjireJ.
O
OctenjakD.
OdehD.
OleninS.
OnofriI.
OrloviA.
OroliA.
OroliN.
Ostoji A.
P
PadoaSchioppaE.
PalijanG.
PalombiB.
PartlA.
PasmansF.
PatevE.
PaviiHamerD.
PavieviM.
PavlicJ.
PavlicaM.
PavlusN.
PavokoviD.
O35
P72
O36,P101
P94
P65
O72
P48,P53
P53
P100
P128
P23
PL3,O41,O42
P12
O47,P26
O21,P22
O118
O75
O125
P122,P132
P69
O78
P17
O110,
P122,P125,
P126,P128,
P129,P130,
P131,P132,
P133
O105
O50
O108
P94
O7
P56
O3,P2
O81,P73
O72
O28
P82
P13,P14
O92,P140
PeanacD.
PehardaM.
PeharectefaniP.
PeraniI.
PeriM.
PericaK.
PerinaD.
PeroPucarD.
PeroviA.
PeroviS.
PeriV.
PeruscoV.S.
PestoriB.
PetekA.
PeternelH.
PetriI.
PetrovRaniI.
PevalekKozlinaB.P85
PiasevoliG.
PicciulinM.
PinteriM.
PircMezgaV.
PiriaM.
PithartD.
PlavacI.
PlazibatM.
PlenkoviMorajA.
PlesliG.
PleaZ.
PlohlM.
PoboljajK.
PodnarM.
PodrugI.
PolakP.
PolakT.
PoloM.
PoloviD.
PoloviL.
PoljuhaD.
PongractimacZ.
PopgeorgievG.
PopijaA.
PopoviDujmoviI.
PopoviHadijaM.
Popovi.
PorejD.
O44
O40
O94,P34
O66
P13,P14
O94
P136,P137
O8
P117
P117
P15
O112
O75
P93
O3
P108
O10
P52
P77
O115
P103
O9,O101
O10
P16
P138
P108
O80,P71,
P74,P76,
P77
O97
P141
P40
IO1,O39,O53
O31
IO5
O85
O50
O40,O41,
O42
O57
O93
O27
P43
P2,P68
O12
P136
P27
O2
th
12.HBK29112 CBC
PosavecVukeliV.
PrakljaiB.
PresterLj.
PreviiA.
PrliD.
ProtrkaK.
PruknerRadoviE.
PtiekA.
PucarB.
PuillandreN.
R
RaanR.
RadanoviI.
RadanoviGuvicaB.
RadiBrkanacS.
RadiStojkoviM.
RadoniI.
RadosavljeviI.
RadoviJ.
RaduloviM.
RaglandG.
RakoGospiN.
RakoviK.
RaspudiE.
RaanL.
RaanM.
RaanS.
RatkajI.
RazdorovG.
RazlogGrlicaJ.
RebrinaF.
ReljiS.
ReetnikI.
ReynoldsA.
RezoM.
RifatbefoviM.
RdelM.O.
RodiP.
RoakE.
RomtajnBurchards.
RosaR.
RozgajR.
RoiS.
RubenI.
RusakG.
P1
O62,O63
O113
P92
P102
P5
P50
P14
O8
O37
P125
O19,O34,
O105
P4
P34,P85
P85
P75,P139
O44,O45
P16
P76
P30
O80,P71,
P76,P77
P138
O97
P103
O26, O33, P25,
P103,P106
O33
P143
O89
P26
O38
O125
O44
IO5
P17,P99
P56
P42
P68
P125,P128
P27
O83
P114
O82
O71
O93,P83
RuiM.
RynesE.
S
SaboliI.
SaboliM.
SabovljeviA.
SabovljeviM.
SadanakuE.
SafnerR.
SamardiM.
SamoilaC.
SandstromR.
SarnoD.
SasiB.
SailoK.
SauerbornKlobuarR.
SchebeckM.
SchneiderD.
SchulerH.
SeilerM.
SepiK.
SepiciDinelA.
SertiPeriM.
SimiT.
SimonoviP.
SiniN.
SinkoviN.
SirovinaD.
SkaramucaB.
SkaramucaD.
SkejoJ.
SkubeK.
SkukanR.
SlavevskaStamenkovikjV.
SloviA.
SmiljkovS.
SnojA.
SomlyaiI.
SoriaCarrascoV.
SpatzT.
SrkoM.
StagliiN.
StamatoyannopoulosJ.A.
StaniP.
StankoviD.
StankoviM.
O31,P36,
P85
IO5
P17
P18,P19
IO3,P86
IO3,P86
O70
O101
P6
O71
IO5
P65
O119
P132
P109,P110
P30
P19
P30
P42
P142
P113
O19
P142
O9
P138
O20
O31,P130,P131,
P133
P124
P124
O38,P90
P140
P20
P104
O115
P104
O72
P94
O41,O42
P42
P28
P70
IO5
P127
O72
O6
th
12.HBK29212 CBC
StaufferC.
StefkovGj.
SteeviD.
SteviF.
StingaPeruscoV.
StipiS.
StojanovskiS.
StojeviZ.
StoltonS.
StrbadI.
StrunjakPeroviI.
SuiI.
SudariBogojeviM.
SumporD.
SunyaevS.R.
SvibenS.
agM.
alamonT.
andaR.
areviT.
ariA.
ariL.
akorS.
atoviE.
atoviZ.
eganS.
egviD.
egviBubiT.
eputM.
ermanLj.
erugaMusiM.
estanoviS.
evoA.
imiD.
kaliD.
koriD.
krijeljR.
kuncaM.
lipogorV.
ljukaS.
ljukiB.
ogoriS.
olaI.
oltiV.
opfI.
orgoA.
otariI.
P30
O44
O44
P93,P105
P62
P77
P104
O125
O2
P106
P109,P110
O1
O96
P25
IO5
P68
O99
O20
O36
P96
P29
O24
O86
P141
O44,O45
P112
P13
P139
P36
P138
O115,O116
P121
P127
O90
P62
O116
O68
P2
O124
O9
P117
P127
O93,P83
P54
P131
PL4
O44
paniR.
pernjakA.
poljarM.
poljariI.
poljariMaroniD.
premN.
rutM.
tagljarI.
tambukA.
tefanA.
tefaniI.
tihA.
ukaloG.
unjeE.
uprahaL.
valjekD.
verL.
T
TadiV.
TadiZ.
TacE.
TemunoviM.
TernjejI.
ThurmanR.
TkalecM.
TomljanoviI.
TomljanoviT.
TomoviLj.
TopiR.
TopiPopoviN.
TreerT.
TrencN.
Trumbi.
TuriN.
TurkoviakaliI.
TuekM.
TutmanP.
TvrtkoviN.
TzankovN.
P20
O30
O105
P108
P105
O101
O40, O41, O42,
O111,P117
PL1
O40, O41, O42,
O111,P134
O2,P9
P28
O73,P91
P51,P55
O68,P56
P65
P13
P4,P5
O119
O58,O124,P59
O109
P88
P108
IO5
O111, P79, P84,
P116
P140
O9,O101
O64,P55
P16
P109,P110
O101
O10,P7
P139
O96,O99,P88
P93
P79,P84
P70
IO1,O38
O65, P43, P45,
P48,P53
U
UlmL.
UroeviA.
P118
O64,P57
th
12.HBK29312 CBC
V
vanLieshoutK.
vanStratenS.
VarbanovaK.
VargaM.
Varga.
VasiliE.
VejselovaD.
VelkiM.
VergilovV.
VidakoviJ.
VidatiL.
VignjeviG.
VilajI.
VinkoviVrekI.
ViiH.
VladoviD.
VlahoviekK.
VlahoviM.
VlaieviB.
VojvodaZeljkoT.
VoloenT.
VondrakT.
VrankoviL.
VrgoS.
VruinaI.
VuciA.
VuciM.
VukoviK.
VuurT.
VujanoviM.
VujiV.
VujiiM.
VujnoviT.
VukovT.D.
VukoviA.
O54,O55
O54,O55
O44
O24
P14
P98
P115,P119
O108
O65
P107
O106
O96,O100,P88
P39,P49,P58
P87
P110
P37,P52,
P64
IO5
P138
P107
P141
O62
O5
O125
P78
O96,O10,
P92
O8
O37,O61,
O63,P38,
P39,P134
P68
O80,P76
P56
P85
P86
P81
O57,O64
P93
VukoviM.
VukoviV.
VuksanoviS.
W
WeissS.
WerneburgI.
WeteringsM.J.A.
Z
ZabricD.
ZadravecM.
ZahiroviV.
Zahirovi.
ZanellaD.
ZdelianJ.
ZimiA.
ZlatkovB.
ZovkoA.
ZovkoM.
ZovkoKoniM.
ZrinakI.
ZubkoviA.
ajaR.
alacS.
eljeiD.
evrnjaN.
ganecK.
iiGuoE.
uljeviA.
unaPfeifferT.
unecR.
utiniP.
P13,P14,P81
O120
O57
O9
P53
O54,O55
O9
O58,O99,
P44,P59,P60
P105
O96,O99,
O100
O36,P101
O97
O52,O59,
P41,P61
O65
P10
P87
O105,P133
O105
P143
P137
P62
P114
P37,P52,P78
O12
P121
O77,O82,P66
P105
O121
P108
th
12.HBK29412 CBC
Napomeneurednika
Saeciodraavajustanovitaautora,aurednicisuispravilisamooitepogrekeutekstovima,
te tehniki uredili i ujednaili izgled saetaka prema zadanom predloku. Kvaliteta i standard
engleskogjezikaodgovornostsuautorajertekstovinisuposebnolektorirani.
Editors'remarks
Theabstractsreflecttheauthorsviewpointsregardingthesubjectandeditorscorrectedonly
obvious errorsin thetexts,andpreformedtechnicalediting.Thequalityandstandardof the
Englishlanguagearetheresponsibilityoftheauthor,asnolanguageeditingofsubmittedtexts
wasdone.
Kratice/Abbreviations:
PLplenarnopredavanje/Plenarylecture
Ousmenoizlaganje/Oralpresentation
IOUvodnotematskopredavanje/Introductorylecture
Pposterskopriopenje/Posterpresentation
th
12.HBK29512 CBC
SPONZORI
SPONSORS
Pokrovitelji/Patrons:
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