Name:

Course & year:

Date:

Score:

Exercise No. 31: IMViC Reactions

1. Indole test
Positve: Red ring at the interface of reagent and broth
Negative: No color development

2. SIM test
Hydrogen sulfide/ Indole/ Motility
A: -/+/+
B: -/-/C: +/-/+
D: -/-/+
E: +/+/+

3. MRVP test
Positive: Distinct red color at surface of the medium
Negative: Yellow color at the surface of the medium

Positive: Pink red color at surface of the medium

Negative: Yellow color at surface of the medium

4. Citrate test
Positive: Intense Blue and presence of growth
Negative: No change in color, absence of growth

1. Indole test
Group result:

Interpretation:

Uninoculated

Inoculated

2. SIM test
Group result:

Interpretation:

Uninoculated

Inoculated

3. MRVP test

Uninoculated

Group result:

Group result:

Interpretation:

Interpretation:

MR tube

VP tube

4. Citrate test

Group result:

Interpretation:

Uninoculated

Inoculated

5. SUMMARY (Group result):

TSIA:
LIA:
IMViC:
Species identified:_________________________