Professional Documents
Culture Documents
Theory:
Materials:
Glucose, yeast, lime water, distilled water, filter paper, mashed pineapple, cooked
potatoes, tap water.
Apparatus:
250 cm3 conical flask, 150 cm3 conical flask, 500 cm3 beaker, 50 cm3 measuring
cylinder, distillation flask, stopper with delivery tube, stopper with one hole,
thermometer, Liebig condenser, fractionating column, retort stands and clamps, tripod
stand, wire gauze, Bunsen burner, rubber tubing, filter funnel, boiling tube.
Procedure:
1. About 20g of glucose was dissolved in 150 cm3 of distilled water contained in a
clean conical flask.
2. About 10g of yeast was added to the mixture and the mixture is shaken well.
3. The conical flask was closed with a stopper connected with a delivery tube. The
other end of the delivery tube was dipped into lime water contained in a boiling tube
as in Figure 1(a).
6. After about a week, the contents of the conical flask are filtered. The filtrate was
poured into a distillation flask.
7. The apparatus for distillation as shown in the figure 1(b) was set up.
8. The filtrate was heated in a water bath and the ethanol that boils over at 78 to
800C was collected.
10. Steps 1 to 9 are repeated using mashed pineapple and cooked potatoes
consecutively to replace glucose.
Observation: