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    Jubair Al-rashid
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    1) The document describes a cloning strategy to insert the PsCBL and PsCIPK genes into plant transformation vectors pRT100 and pRT101. 2) The strategy involves inserting the PsCBL and PsCIPK genes in both sense and antisense orientations downstream of the CaMV 35S promoter between the right and left borders of pBI121. 3) Selection markers like NPT II and GUS are also included in the vectors to enable selection of transformed plants.

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    Cloning strategy of PsCBL and PsCIPK gene in plant transformation vector

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    1) The document describes a cloning strategy to insert the PsCBL and PsCIPK genes into plant transformation vectors pRT100 and pRT101. 2) The strategy involves inserting the PsCBL and PsCIPK genes in both sense and antisense orientations downstream of the CaMV 35S promoter between the right and left borders of pBI121. 3) Selection markers like NPT II and GUS are also included in the vectors to enable selection of transformed plants.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
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    8 views1 page

    Strategy

    Uploaded by

    Jubair Al-rashid
    1) The document describes a cloning strategy to insert the PsCBL and PsCIPK genes into plant transformation vectors pRT100 and pRT101. 2) The strategy involves inserting the PsCBL and PsCIPK genes in both sense and antisense orientations downstream of the CaMV 35S promoter between the right and left borders of pBI121. 3) Selection markers like NPT II and GUS are also included in the vectors to enable selection of transformed plants.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 1

    Cloning strategy of PsCBL and PsCIPK gene in plant transformation vector

    A
    CBL CIPK

    pRT100 pRT101
    CBL CIPK
    Hind III site Hind III site
    EcoRI Xho I

    Ca MV 35S Poly A Ca MV 35S


    Poly A
    (Anti-Sense CBL) CBL gene (Anti-Sense CIPK) CIPK gene

    Nco I BamHI EcoRI


    Nco I

    Ca MV 35S Poly A Ca MV 35S


    Poly A
    (Sense CBL) CBL gene
    (Sense CIPK) CIPK gene

    Hind III Hind III


    NOS pro NOS pro NOS Ter NOS Ter
    NOS Ter NOS Ter Right border LB
    LB RB
    RB

    NPT II (Kana) Ca MV 35S GUS gene NPT II (Kana) Ca MV 35S GUS gene
    promoter promoter
    Bam H 1 Bam H 1

    pBI121 pBI121

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