3’ Biotin, 3’-Biotin CPG

AHP-CH-Bio0103 100 mg Coupling: Proceed in a manner identical to the use of normal

AHP-CH-Bio1003 1000 mg nucleoside support since it contains the DMT group.
Name: 3’-Biotin CPG
Chemical Name: 1-Dimethoxytrityloxy-3-O-(N-biotinyl- Deprotection: Pretreat with diethylamine or DBU for 30
3-aminopropyl)-propyl-2-O-succinoyl-long chain alkylamino- minutes at room temperature. Then use ammonium hydroxide
CPG to cleave the oligonucleotide. This requires 2 hours at room
Formula: N/A temperature. Use an appropriate protocol (depending on the
nucleobases) to complete the deprotection.

Storage: Freezer storage, -10 to -30°C, dry, dark area

Labeled Oligo Storage: Store labeled oligo in the dark, either

dry or in a neutral aqueous media at -20°C. Do not store crude
fluorescently labeled oligonucleotides using ammonia.

MSDS: www.allelebiotech.com

Reference:

Iezzoni JC, Kang JH, Montone KT, Reed JA, Brigati DJ. Colorimetric
detection of herpes simplex virus by DNA in situ sandwich hybridiza-
tion: a rapid, formamide-free, random oligomer-enhanced method. Nuc
Acids Res., 20, 1149-1150. (1992)

Diamandis EP, Christopoulos T.K. The biotin-(strept)avidin system:

Principles and applications in biotechnology. Clinical Chemistry, 37,
Use: 3’-Biotin CPG is used to label Biotin at the 3’ end of oligo- 625-636. (1991)
nucleotides
Purity: 95%+
Diluent: N/A, Stability in Solution: N/A

Background:

Biotin labeling can use to detect proteins using various methods

(i.e., non-radioactive immunoassays, cytochemical staining, cell
separation, isolation of nucleic acids, detection of specific DNA/
RNA sequences by hybridisation, probing of conformational
changes in ion channels).

Many reagents are available for labeling nucleic acids with bio-
tin. Biotin-CE Phosphoramidite can add multiple biotins to either
the 3’ or 5’ end of an oligonucleotide. Similarly, Biotin-TEG-CE
Phosphoramidite adds biotin to the 3’ and 5’- ends of an oligo.
5’-Biotin-CE Phosphoramidite and 5’-Biotin-CE Phosphoramid-
ite II can only add biotin to the 5’-end while 3’-Biotin CPG only
works at the 3’-end. For internal addition of biotin, Biotin-dT-CE
Phosphoramidite is suggested.

It should be noted that the DMT group of 3’-Biotin CPG can

be used for cartridge purifications. There is potential for cross
contamination in HPLC purifications--which should be avoided
when preparing biotinylated PCR primers.