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Lecture 23 Bordetella
Lecture 23 Bordetella
GENUS:
BORDETELLA
Prof. Khalifa Sifaw Ghenghesh
Small, Gram-negative coccobacilli
Strict aerobes.
X and V factors not required for
growth
Three species:
–B. pertussis,
–B. parapertussis
–B. bronchiseptica
Bordetella species
Bordetella bronchiseptica
Leifson flagella stain
Scanning electron micrograph of
Bordetella bronchiseptica
Bordetella pertussis
Whooping Cough
– Paroxysmal coughing
Require blood, or starch or
charcoal for growth
– Bordet-Gengou medium (BGM)
Strict aerobe
Optimal temp. > 35-36oC
ANTIGENICITY
Identification:
– Serological
2. Detection of Bacterial Antigens
Bordetella antigens in serum and
urine using specific antiserum
Immunofluorescence Technique:
– Bacteria in nasopharyngeal
secretions labelled with fluorescein-
conjugated antiserum > examined
with ultraviolet microscopy.
3. Detection of Bordetella
Antibody:
Sera and nasopharyngeal secretions
examined for Ab > Agglutination
Tests, ELISA
Bordetella pertussis
TREATMENT
Erythromycin
–For 2 weeks
–May reduce the severity of
illness if given before
paroxysmal stage.
–No clinical effect when the
infection is established
–May be given to protect non-
vaccinated infants
CONTROL
Vaccination:
– Safe and >90% effective.
– Adsorbed vaccine (i.e. with adjuvant).
- Contains all 3 agglutinogens.
- Minimum 3 doses.
- Minor adverse reactions:
- Erythema and local swelling, slight
feverishness
- Possible neurological sequelae
Bordetella bronchiseptica
–a cause of pneumonia, otitis
media, and other respiratory
infections in animals.