BIOL 350 FINAL EXAM FALL 2013

BIOL 350: General Microbiology

FINAL EXAM
VERSION A
Before beginning:
ri!e yo"r na#e a! !$e !o% o& !$i' co(er '$ee!) *o no! re#o(e !$e '!a%le)
ri!e yo"r na#e an+ re+ I* on yo"r 'can!ron)
ri!e +o,n ,$ic$ e-a# (er'ion yo" $a(e .no!e color o& !o% '$ee!/
0$ec1 !o #a1e '"re !$a! yo"r !e'! $a' all 50 2"e'!ion')
Read each question carefully.
This exam is worth 100 points. Each question is worth 2 points.
ood luc!"

BIOL 350 FINAL EXAM FALL 2013
1. #hat is TR$E of %T&ase'
%. (s composed of three primary subunits
B) 3$e F4 '"b"ni! c$ange' A*5 !o A35
). (t transfers electrons to oxygen in aerobic systems
*. %ll of the abo+e are true
E. ,one of the abo+e are true

2. #hat is TR$E of the E. coli Electron Transport -ystem .ET-/0
%. E. coli uses the same substrate oxidoreductase for all electron
donors
B. The terminal oxidoreductase reduces $biquinone
). 1xygen can be an electron *1,1R
*. The %T&ase creates an 23 gradient
E) None o& !$e abo(e are 3R6E
4. Rubisco cataly5es the reaction0
A) 0O7 8 rib"lo'e94:59bi'%$o'%$a!e 9; 7 %$o'%$oglycera!e
B. 2)1 3 acetyl6)o% 67 malonyl6)o% 3 ,%*&2 67 46
hydroxypropionate
). 22- 3 light 67 -0 3 2 23 3 2 e8
*. )12 3 221 67 )2214
E. ,one of the abo+e
9. TR$E or :%;-E0 %T& directly phosphorylates the :e<o-
cluster to brea! ,2 bonds.
%. TR$E
B) FALSE
=uestions >6?0 <atch each of the organisms with a food. 1nly one
answer per question@ but a letter may be used more than once or
not at all.
(A) Leuconostoc
(B) Proiono!"cteriu#
($) Por%&r"
(') L"cto!"cillus
0 >. -ushi
B A. -wiss )heese
A B. -auer!raut
* ?. Cogurt
D. Experimental e+idence for early life includes the fact that0
%. :e- compounds can cataly5e the fixation of nitrogen in a self6
sustaining reaction
B) S#all organic #olec"le' &or# abio!ically in <%rebio!ic 'o"%=
,i!$ a li!!le elec!rici!y
). &roteins can spontaneously form spherical micelles
*. 1xygen ma!es a great electron acceptor in test tube
experiments
E. ,one of the abo+e
10. The temperature of water )%,,1T drop below free5ing and
sustain life because0
%. *,% will fall apart
B. nutrient transport systems do not wor!
). %T& will become crystalline
*. plasma membrane will free5e
E) en>y#a!ic reac!ion' canno! be #ain!aine+
=uestions 116190 <atch each of the organisms with the BE-T
match below.
(A) <ethanogen
(B) *esulfurococcales
($) 2alophiles
(') )renarcheota
B 11. ,o cell wall
* 12. -ometimes psychrophilic
0 14. Rhodopsins 8 All "ns(ers "ccete) *or +13 since I )i) not
co,er t%is "sect o* %"lo%iles.
A 19. Electron acceptor )12
=uestions 1>61?0 <atch each of the organisms with the BE-T
match below.
(A) 1pistho!onts
(B) Brown %lgae
($) %moebo5oa
(') iardia
* 1>. ;ac! mitochondria
B 1A. Energy storage lipid
0 1B. -lime <old
A 1?. (nsertion in common gene sequence
1D. #hich of the following is TR$E of smallpox'
%. (t probably !illed 4064>E of the nati+e peoples in the %mericas
B. (t is curable by antibiotics
). *uring #orld #ar ( it !illed more than the war itself
*. (t Ftra+elledG down the -il! Road from )hina
E) 3$e (ir"' !arge!' '1in cell'
20. TR$E or :%;-E0 :lu !illed more people than weapons did in
#orld #ar (.
A) 3R6E
B. :%;-E
21. #hat is true about the intestine and its microflora'
A) 3$e (a'! #a?ori!y o& $"#an9a''ocia!e bac!eria li(e $ere)
B. The low p2 of impedes bacterial growth.
). -tretococcus are dominant here.
*. .elico!"cter &lori commonly infect intestine walls.
BIOL 350 FINAL EXAM FALL 2013
E. (t selects for salt6tolerant bacteria.
22. #hat is TR$E of the human genitourinary microflora'
A) Lactobacillus can %re(en! e'!abli'$#en! o& %a!$ogen'
B. Proion!"cteri" are abundant in this microflora
). 2ealthy bladders are full of bacteria
*. The microflora needs to tolerate high p2
E. ,one of the abo+e.
24. #hich of the following is part of the s!in condition called
acne0
%. % pathogenic +irus in+ades the hair follicle
B. The causal agent feeds on hormones release in puberty
). 2alophiles grow on salty s!in causing the rash
*) 3$e ca"'al agen! i' &o"n+ on nor#al $eal!$y '1in
E. ,one of the abo+e
29. #hich of the following describes the symbiotic relationship
!nown as a #utu"lis#'
%. both species benefit in an unspecific manner
B. one species preys upon another
). one species benefits while the other is harmed
*. one species benefits while the other is unaffected
E) bo!$ '%ecie' bene&i! an+ +e%en+ on eac$ o!$er
2>. TR$E or :%;-E0 /%i0o!iu# coloni5e the cells of tree roots.
(Accete) !ot% "ns(ers /%i0o!iu# coloni0e le1u#es (!e"ns)2 !ut
t%ose c"n !e trees (e3"#le4 Ac"ci" tree)
%. TR$E
B) FALSE
2A. #hich is the counterstain in the ram stain procedure'
%. crystal +iolet
B. methylene blue
). malachite green
*) 'a&ranin
E. ramHs iodine
2B. The following rod shaped bacterium will be what color after
ram staining'
%. -taphylococcus@ purple.
B. Bacillus@ pin!.
). -treptococcus@ purple.
*. -almonella@ purple.
E) E'c$eric$ia coli: %in1)
2?. #hat part of a cell is contains peptidoglycan'
%. nucleoid
B. cytoplasm
). periplasmic membrane
*) cell ,all
E. outer membrane
2D. #hich name refers to ha+ing flagella attached all around the
cell'
A) %eri!ric$o"'
B. lophotrichous
). monotrichous
*. bitrichous
E. flagellated
40. %n al!alophilic organism requires which of the following
condition.s/ to grow'
A) a %@ be!,een A944)
B. a high le+el of oxygen.
). high concentration of ,a)l.
*. a temperature of 0
o
) 8 20
o
).
E. can grow at both high and low le+els of oxygen.
41. The lipopolysaccharide of the outer membrane is of medical
importance because it acts as a.n/ IIIIIIIIIIII.
%. speciali5ed phospholipid.
B) en+o!o-in)
). adhesin
*. capsule polysaccharide.
E. Both B and *
42. *uring which stage of bacterial growth is the culture growing
exponentially'
%. lag phase
B) log %$a'e
). death phase
*. stationary phase
E. both % and B
BIOL 350 FINAL EXAM FALL 2013
44. %n organism that requires inorganic compounds as sources of
energyJelectrons is specifically called aJanIIIIII'
%. &hototroph
B. 2eterotroph
0) Li!$o!ro%$
*. 1rganotroph
E. %utotroph
49. 2uman pathogens are
%. halophiles.
B. psychrophiles.
0) #e'o%$ile')
*. thermophiles.
E. extreme thermophiles.
4>. #hich of the following is a dormant form of bacteria resistant
to desiccation and other natural stresses'
%. ribosome
B. carboxysomes
). storage granules
*. thyla!oids
E) en+o'%ore
4A. #hich of the following mutagens may cause Thymine dimers'
%. K6rays
B. >6bromouracil
). nitrosoguanidine
*) 6V ra+ia!ion
E. ethidium bromide
4B. #hich of the following forms of transmission specifically
requires cell6to6cell contact for *,% transfer to occur'
%. speciali5ed transduction
B. replicati+e transposition
). transformation
*. general transduction
E) con?"ga!ion
4?. #hich of the following forms of transmission specifically
requires bacteriophage'
A) !ran'+"c!ion
B. replicati+e transposition
). transformation
*. general recombination
E. conLugation
4D. #hich molecule is responsible for the initial opening of the
*,% helix during *,% replication'
%. *,% primase
B. single6stranded binding protein
). *,% polymerase (((
*) *naA
E. *naB
90. #hen a bacterial culture is growing@ under which of the
following conditions will )atabolight Repression occur'
A) Gl"co'e %re'en!: Lac!o'e %re'en!
B. 1nly ;actose present
). lucose absent@ ;actose present
*. lucose absent@ ;actose absent
E. B and ) only
91. #hich process of the bacterial cell ser+es as an ideal target for
antibiotic therapy'
%. cell wall synthesis
B. *,% replication
). protein synthesis
*. Transcription
E) all o& !$e abo(e
92. %n antibiotic which !ills the bacterium is considered to be
what'
A) bac!erici+al
B. broad spectrum
). narrow spectrum
*. bacteriostatic
E. all of the abo+e
BIOL 350 FINAL EXAM FALL 2013
94. #hich bacterium causes meningitis primarily in newborns
being passed from the mother to the infant'
%. -treptococcus pneumoniae
B. 2emophilus influen5ae
). ,eisseria meningitidis
*) Gro"% B '!re%!ococc"'
E. ;isteria monocytogenes
99. Beta lactamases wor! by which mechanism to achie+e
antibiotic resistance'
%. addition of modifying groups that inacti+ate the antibiotic
B. efflux pumps
0) +e'!r"c!ion o& !$e an!ibio!ic
*. modification of cell target protein
E. all of the abo+e
9>. &enicillin inhibits what component of cell wall synthesis'
%. autolysins.
B) !ran'%e%!i+a'e')
). transglycosidases.
*. bactoprenols.
E. ,6acetylglucosamines.
9A. #hich part of the cellular innate defense system is !nown as
the Fbig eaterG responsible for !illing microbes'
%. neutrophil.
B. natural !iller cell.
). B cells.
*) #acro%$age.
E. dendritic cell.
9B. #hich is the largest &hysical barrier to infection'
%. mucous.
B) '1in)
). cilia escalator.
*. toll li!e receptors.
E. +omiting and diarrhea.
9?. #hich of the following bacterium is associated with
gastrointestinal tract infections@ and specifically stomach ulcers'
%. $"#&lo!"cter 5e5uni
B. B"cillus "nt%r"cis
). -t"%&lococcus "ureus
*) Helicobacter pylori
E. Enteroin+asi+e E. coli
9D. #hich of these reactions in the process of ma!ing beer releases
energy .%*& %T& and ,%*3 ,%*2/'
%. :ermentation
B) Glycoly'i'
). The Mrebs )ycle
*. %lcoholation
E. &hotosynthesis
>0. #hich of the following bacterium has been used as a bio6
weapon'
%. $"#&lo!"cter 5e5uni
B) Bacillus anthracis
). -t"%&lococcus "ureus
*. .elico!"cter &lori
E. Enteroin+asi+e E. coli