Effect of Vitamin C and anti-

oxidative nutrition on radiation-

induced gene expression in
Fukushima nuclear plant workers

Atsuo Yanagisawa, M.D., Ph.D.

Japanese College of IV Therapy

1
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There are many scientific studies
demonstrate protective effects of
Vitamin C upon radiation-induced
cellular injury .
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Fig. 1. Mouse survival after whole body radiation (A). Mice were irradiated at 6, 8, 10, 12, and 14 Gy. The
effect of boneM0);,%*0)+4%;).@3@,%+,;;%2<-(%2<-(%&%GF%1<0+,%A04F%3..-43-:0(%
marrow transplantation on mouse survival after radiation (B). Mice were irradiated at 10, 12,
NOPHQ8%P0(,%B-..01%2.-(;/+-(2-:0(%NPMRQ%"S%<%-D,.%3..-43-:0(%
and 14 Gy. Mice received BMT 24 h after radiation. N = 12 in each group. *P < 0.01 vs. other groups.
.,;*),4%B3*,%.,*,3@3(>%+,;;%2<-(%5"%GF8%E01,@,.C%(0%B3*,%.,*,3@3(>%
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 Prevention of Radiation GI Syndrome by Ascorbic Acid

Fig. 3. The K32-B3(%L%FEGD10&H%&H+#>I11-;%0.-++F%-4B3(3;2,.,4%?0.%Z%4-F;C%

effect of treatment with ascorbic acid on mouse survival after radiation. The mice were irradi-
ated at 14 Gy -(4%2<,(%2<,%B3*,%)(4,.1,(2%5S%GF%3..-43-:0(8%9.,2.,-2B,(2%
and received BMT 24 h after radiation. They received ascorbic acid p.o. for 3 days either before
or after radiation, or received distilled water only before radiation. The mice pretreated with ascorbic acid
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were also irradiated at 14 Gy without BMT. N = 12 in each group, *P < 0.01 vs other groups.
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It is our strongest recommendation that those

living in the effected areas regularly take
antioxidant supplements such as vitamin C to
counteract the negative consequences of long-
term low dose radiation exposure as well as to
protect the health of coming generations.
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 Japanese College of
Intravenous Therapy
(JCIT)
We are 400 members: 379 physicians
12 dentists
9 veterinarians

More than 10 educational seminars every year.

IV Vitamin C, IV Lipoic acid, IV Chelation,
Myers Cocktail, Ukrain, Nutrition therapy, etc.

24
The 1st JCIT Symposium of IVC for Cancer Patients
Oct 17, 2010, Tokyo, Japan "#!
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26
Effect of Vitamin C and anti-
oxidative nutrition on radiation-
induced gene expression in
Fukushima nuclear plant workers
- A pilot study

Atsuo YANAGISAWA, M.D., Ph.D. (1)

Michie IWATA, M.D., Ph.D. (2)
Shuichi AKIYAMA (3)

(1) Japanese College of IV Therapy

(2) Iwata Ladies Clinic,
(3) Gene Science Co, Ltd.
27
 H(2.04)*:0(%
In the statement of Environmental Radioactivity and Health
issued in March 29, 2011, we strongly recommended that
People living in the effected areas should regularly take
antioxidant supplements such as vitamin C to counteract the
negative consequences of long-term low dose radiation
exposure as well as to protect the health of coming
generations. However, TEPCO, government and all medias
ignored our statement.

Recently, we examined cancer-related gene expression in 16

men who had worked at severe radiation environment at
Fukushima nuclear plant. We also confirmed the improvement
of cancer risk score by the supplementation of Vitamin C and
other anti-oxidative nutrition.

28
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29
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31
 F Concentration
Free DNA concentration (ng/mL plasma)

200

175 Cut-off value <25ng/mL

150 >25ng/mL
Healthy control 17% (n=400)
125 Cancer patients 38% (n=400)
100

75

50

25

0

32
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3(%n)[);<3B-%O0.[,.;%

Plasma F 
Cancer Risk Score
ng/mL


"#! M! KL!

!. !.

Cancer Risk Score is calculated from the value of 48 cancer-

related gene expression using logistic analysis.
34
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ab'%-(4%L-(*,.%V3;[%`*0.,%3(%n)[);<3B-%O0.[,.;8%%

Cancer Risk Score

ng/mL
F

"#! KL!
NO.B.#

!. !.
Before 2M Before 2M

Five workers were treated with IVC twice a month and oral anti-oxidative
nutritional supplements.  35
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()*+,-.%/+-(2%10.[%-2%n)[);<3B-8%
ng/mL F Cancer Risk Score
KL!
"#!

!. !.
Before After work  Before After work 

Four workers took once i.v. Vitamin C (25g) therapy at before they went, and
continuously took anti-oxidative supplements during the working period. No
significant change was seen in both Free DNA and Cancer Risk Score.
36
 L0(*+);30(%
(1) We evaluated the effects of radiation exposure on cancer
related gene expression in 17 men who worked 5-6 weeks at
the radiation contaminated area of Fukushima nuclear plant.
(2) After working, plasma free DNA was increased in 2 of 13
workers, and cancer risk score was increased in 3. After the
2 months intervention with intravenous Vitamin C and oral
anti-oxidative nutritional supplements, free DNA returned to
normal level and cancer risk score was significantly
decreased (p<0.05).
(3) No significant change was seen in 4 workers who took once
i.v. Vitamin C at before working and continuous intake of
anti-oxidative supplements during the working period. 
(4) Increase in cancer risk by radiation exposure can be
protected by anti-oxidative nutritional intervention.
Fukushima workers in the radiation contaminated area
should take anti-oxidative nutrition therapy immediately. 37
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 Thank you !

http://www. iv-therapy.jp
P"!