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Ratification Page: Mikroskop" That Arranged by
Ratification Page: Mikroskop" That Arranged by
Known by,
Responsibility lecture
Drs. Hamka L, MS
NIP : 19621231 198630 0 015
CHAPTER I
INTRODUCTION
A. Background
Development of science in the era of globalization to move fast. One of
example is biology. In the world is not all things can be seen with the naked eye,
because there is a limit of human vision. So that needed a tool, and the tool called
a microscope. Therefore, many problems concerning objects or organisms to be
observed and the observations can only be done by using the tools. One tool that
is often used in research or observations about organisms that can not be seen with
the eye, is a microscope.
The microscope is one of tool that is widely used in the laboratory or
process Biology lab activities. The person who first used the microscope in the
form of a simple though is Antony Van Leuwenhoek in the field of microbiology.
Then in 1600 Hans and Z Jansen has found a more advanced microscope with the
name of a compound microscope. Microscope comes from the word meaning
small and micro scopium (sight). Microscope is a tool that studies the structure of
the objects to be noticeable by the naked eye.
The microscope uses light called an optical microscope, the microscope is
generally used in laboratory school. There are two types of optical microscopes,
namely are biological microscope and stereo microscope. Microscope in principle
consists of two lenses are as objective lens and the ocular lens. Each objective
lens can be rotated into place in accordance with the desired magnification.
Magnification objective lens system provides early and produce a real image
which is then projected onto the ocular lens. The visual image was magnified by
the ocular to produce a virtual image that we see.
The microscope has a perishable components, such as lenses and mirrors,
these components need to be treated well and good care anyway, so we should
avoid treatments that can cause damage such as rubbing the lens with a rough
cloth. Therefore, practical introduction and how to use the microscope is very
necessary.
B. Purpose
Students are skilled in using of the microscope with a fast and safe to see
the simple preparation
C. Benefits
Add knowledge and insight about how to use a microscope, knowing the
parts of the microscope and their respective functions, and then knowing the
structure of cell and tissues that exist in plants in accordance with the
experiments performed
CHAPTER II
PREVIEW OF LITERATURE
The evolution of science have often been aligned with the discovery of
equipment that expands the human senses to be able to enter new boundaries.
discovery and initial studies about cells to make progress in line with the
invention and refinement of the microscope in the seventeenth century. Some
microscopes still become an indispensable tool in studying the cell.
The microscope was first used by scientists Renaissance, and also is a microscope
that is used to this day in the laboratory, is a light microscope (LM light
microscope) of visible light is passed through a specimen and then penetrate the
glass lens. This lens merefraksi (bend) light such that the specimen magnified
shadow shadow when it is projected into our eyes. (Campbell Neil. A. 2000)
Two important values is the magnification of a microscope and
decomposition, or resolution. Enlargement reflects the number of times larger
than the size of the object look real. The resolving power is a measure of the
clarity of the image, which is the minimum distance of two points which can be
separated and still be distinguished as two separate points. For example, what is
visible to the naked eye as a star might just break down into a binary star with a
telescope. Just as the power of the human eye is limited explained, the microscope
is also limited. The microscope can be designed to magnify objects as big as you
want, but never decipher microscope finer details. Decomposition or resolution is
limited by the wavelength of visible light is used to illuminate the specimen. Light
microscope can magnify effectively until approximately 1000 times the size of the
actual specimen. Magnification will increase the opaqueness. (Campbell Neil. A.
2000)
The microscope is one of the necessary equipment in the science
laboratory. This tool is typically used to conduct observations of objects that are
microscopic, both stationary objects or microorganisms that can move. Activity
using a microscope observation of the object / microorganisms either not moving
or moving, requires expertise, precision, patience and accuracy as well as high
concentrations. Trouble finding the object being observed, often makes an
observer to despair, thus terminating its activity without results in accordance with
the purpose of observation. (Sadina,2013)
Biological microscope has a maximum magnification of 1000x. This
microscope has the legs are relatively heavy and sturdy to be able to stand stably.
Light microscopy is also a three-lens system, the objective lens, eyepiece and
condenser. The objective lens and eyepiece located at both ends of the tube
microscope. Eyepiece on the microscope can be shaped single lens / monocular
and dual binocular. At the lower end of the light microscope, there are objective
lens holder that can be mounted 3 or more lenses with varying magnification.
Under the microscope tube contained dosage table which is where preparations
will be observed. Condenser lens system which serves to illuminate the
observation object and the lens of the microscope to another. In a conventional
light microscope, light source comes from sunlight reflected by a flat or concave
mirror located under the condenser. This mirror will direct the light from the
outside into the condenser. On a more modern light microscopy, the light source
comes from an electric lamp. (Sadina,2013)
Objective lens and eyepiece must be handled with special care. they must be
protected from dust and must do not rub the lens with a cloth or plain paper / rude
because that will make the lens is graze. And then after used, cleaning the
microscope and save in its place. The work table of the microscopy should be of
the large size. You will need the surface not only to set up the microscope, but
also to prepare the preparat, draw and perform many jobs of the practicum. The
work table must be near at a window. if you intend to work in daylight, the
curtains should be remove from the window because the inference with
microscopy and shut off an unbelievable amount of light. (Stehli Georg DR,
1970).
In the guide book of basic biologys practicum 2016, the optical microscope
can be divided into biological microscopes and stereo microscope. Biological
microscope is used for observation of thin transparent objects. Irradiation is given
from the bottom with natural light or lights. The biological microscope has two
lens, namely are ocular lens and objective lens with a magnification power as
follows:
1. 4x objective with 10x eyepiece, magnification 40x
2. 10x objective with 10x eyepiece, magnification 100x
3. 40x objective with 10x eyepiece, magnification 400x
4. 100x objective with 10x eyepiece, magnification 1000x
Objective of the most powerful optical microscope 100x objective emersi
called, because its use should the emersi oil, and how to use it with special
anyway. (Guide Book of Basic Biologys Practicum,2016).
Stero microscope used for the observation of objects that are not too large,
transparent or non-transparent. Irradiation can diataur from above or from below
with natural light or lights. Has two objectives and two ocular, in order to obtain
three-dimensional image with the observation of both eyes. Magnification power
of the lens is not too strong, generally as follows: 1x or 2x objective with the 10 x
or 15 eyepice. microscope has the components of the glass are easily damaged,
such as lenses and mirrors. avoid treatments that may damage these components,
such as avoiding conflicts. (Guide Book of Basic Biologys Practicum,2016).
A microscope is an instrument that magnifies an object. The optics of the
lenses of a light microscope changes the orientation of the image. A specimen that
is right-side up and facing right on the microscope slide will appear upside-down
and facing left when viewed through a microscope, and vice versa. Similarly, if
the slide is moved left while looking through the microscope, it will appear to
move right, and if moved down, it will seem to move up. This occours because
microscopes use two sets of lenses to magnify the image. Due the manner in
which light travels through the lenses, this system of lenses produces an inverted
image (binoculars and a dissecting microscope work in similar manner, but
include an additional magnification system that makes the final image appear to
be upright). Most student microscopes are classified as light microscopes. Visible
light both passes through and is bent by the lens system to enable the user to see
the specimen. Light microscopes commonly used in the undergraduate collage
laboratory magnify up to approximately 400 times. (Price Gareth, 2006).
CHAPTER III
PRACTICUM METHOD
A. Observation Result
1. Microscope 1. Eyepiece
2. Tube
3. Revolver
4. objective
lens
5. stage
6. stage clip
7. condenser
8. diaphragm
9. mirror
10. base
11. inclination
point
12. arm
13. fine focus
14. coarse
focus
Picture Comparison picture Note
1. Nukleus
2. Material : Allium cepa
2. Sel wall
Magnification : ok = 5X,
ob = 10/0,25
A. Conclusion
Based on the test result that had be done, it can be concluded that :
1. The microscope is an instrument used to observe objects that are very
small (mikroscipic object), making it easy to study.
2. The most common microscope is an optical microscope comprising
biological microscope and stereo microscope.
3. Biological Microscope ocular lens and objective lens that has a certain
magnification strength.
4. There are parts of the microscope, each of which has the function that
helps the observation of small objects.
5. With observations using the microscope, we can see and observe the
nukleus, stomata, the cell wall and tricomes at the objects we have observe
B. Suggestion
In observing and making preparations, preferebly we are more throghhly
in order to obtain clearer date, and then more actively for asking about the
things that are not understandable to the assistant, so there is not error in
observing and making the final outcome of the procces praticum
REFERENCES