winCATS Planar Chromatography Manager

Analysis Report
Method D:\TLC SCANNER\My Documents\CAMAG\METODE\Scan
PG.cme
Created by FITOKIMIA Wednesday, December 29, 2010
1:22:22 PM
Last modified by Biologi Farmasi UGM Wednesday, October 08, 2014
10:03:14 AM
SOP document
Validated Design
Description :

Analysis D:\TLC SCANNER\My Documents\CAMAG\DATA\20141008-

Tempuyung G1 Kel 3.cn
a

Created/used by Biologi Farmasi UGM Wednesday, October 08, 2014

10:09:20 AM
Current user Biologi Farmasi UGM

Stationary phase
Executed by Biologi Farmasi UGM Wednesday, October 08, 2014
10:06:17 AM
Plate size (X x Y) 10.0 x 10.0 cm
Material TLC plates silica gel 60 F 254
Manufacturer E. MERCK KGaA
Batch
GLP code
Pre-washing No
Modification No

Definitions - Screening
Executed by Biologi Farmasi UGM Wednesday, October 08, 2014 10:04:50 AM
Batch Product
Substance name Rf Window size Manufacturer number Expiry date number

AutoGenerated1 0.34 0.500

AutoGenerated2 0.23 0.500
AutoGenerated3 -0.02 0.500
AutoGenerated4 0.11 0.500

Detection - CAMAG TLC Scanner 3

Information
Application position 10.0 mm
Solvent front position 90.0 mm

Instrument CAMAG TLC Scanner 3 "Scanner3_140718" S/N 140718 (1.14.28)

Executed by Biologi Farmasi UGM Wednesday, October 08, 2014 10:06:17 AM
Number of tracks 1
Position of first track X 46.8 mm
Distance between tracks 0.0 mm
Scan start pos. Y 3.8 mm
Scan end pos. Y 44.2 mm
Slit dimensions 8.00 x 0.40 mm, Macro
Optimize optical system Light
Scanning speed: 20 mm/s
Data resolution: 100 m/step

User : Biologi Farmasi UGM Approved :. . . . . . . . . . . . . . . . . . . SN 1410W025, V1.4.3

Wednesday, October 08, 2014 10:10:06 AM Report ID : 07DE0A08040A0914 Page 1 of 5
 winCATS Planar Chromatography Manager

Measurement Table
Wavelength 254
Lamp D2 & W
Measurement Type Remission
Measurement Mode Absorption
Optical filter Second order
Detector mode Automatic
PM high voltage 301 V

Detector properties
Y-position for 0 adjust 3.8 mm
Track # for 0 adjust 0
Analog Offset 10%
Sensitivity Automatic (39)

Integration
Properties
Data filtering Savitsky-Golay 7
Baseline correction Lowest Slope
Peak threshold min. slope 5
Peak threshold min. height 10 AU
Peak threshold min. area 50
Peak threshold max. height 990 AU
Track start position 5.0 mm
Track end position 42.3 mm
Display scaling Automatic
All tracks at Wavelength

800

[ AU ]

600

500

400

300

200
100
100 [ mm ]
0 80
-0.10 70
-0.05 60
0.00
0.05 50
0.10 40
0.15
0.20 30
0.25
0.30 20
0.35 10
[ Rf ]
0.45 0

User : Biologi Farmasi UGM Approved :. . . . . . . . . . . . . . . . . . . SN 1410W025, V1.4.3

Wednesday, October 08, 2014 10:10:06 AM Report ID : 07DE0A08040A0914 Page 2 of 5
 winCATS Planar Chromatography Manager
Track 1, ID:
00 800

00 700

00 600

00 500

00 400

00 300
AutoGenerated1
AutoGenerated2 @ 254 nm @ 254 n
AutoGenerated3 @ 254 nm
00 200 AutoGenerated4 @ 254 nm

00 100

0 0
-0.13 -0.03 0.07 0.17 0.28 0.38 0.47 -0.13 -0.03 0.07 0.17 0.28 0.38 0.47
Start Start Max Max Max End End Area
Peak Rf Height Rf Height % Rf Height Area % Assigned substance
1 -0.06 6.5 -0.02 168.5 25.11 0.03 4.3 5679.3 23.67 AutoGenerated3
2 0.06 0.4 0.11 123.2 18.37 0.15 6.5 4272.3 17.81 AutoGenerated4
3 0.18 2.8 0.23 189.1 28.19 0.28 0.3 6986.1 29.12 AutoGenerated2
4 0.29 5.0 0.34 190.1 28.33 0.40 0.2 7053.7 29.40 AutoGenerated1

Spectrum scan
Executed by Biologi Farmasi UGM Wednesday, October 08, 2014 10:08:19 AM
Mode All detected peaks
Slit dimensions 8.00 x 0.40 mm, Macro
Optimize optical system Resolution
Scanning speed 100 nm/s
Data resolution 1 nm/step
Reference spectrum, pos X 46.8 mm
Reference spectrum, pos Y 3.8 mm

Measurement Table
Lamp D2 & W
Start wavelength 200 nm
End wavelength 500 nm
Measurement type Remission
Measurement Mode Absorption
Optical filter Second order
Detector Mode Automatic

Detector properties
Y-position for 0 adjust 0.0 mm
Track # for 0 adjust 0
Analog Offset 10%
Sensitivity Automatic (39)

AutoGenerated1 on all Tracks

100.0 100.0
[ AU ] [ AU ]
80.0 80.0
70.0 70.0
60.0 60.0
50.0 50.0
40.0 40.0
30.0 30.0
20.0 20.0
10.0 10.0
0.0 0.0
200.0 250.0 300.0 350.0 400.0 [ nm ] 500.0

User : Biologi Farmasi UGM Approved :. . . . . . . . . . . . . . . . . . . SN 1410W025, V1.4.3

Wednesday, October 08, 2014 10:10:06 AM Report ID : 07DE0A08040A0914 Page 3 of 5
 winCATS Planar Chromatography Manager
T Rf Substance Max. @
1 0.34 Rf AutoGenerated1 221 nm

AutoGenerated2 on all Tracks

100.0 100.0
[ AU ] [ AU ]
80.0 80.0
70.0 70.0
60.0 60.0
50.0 50.0
40.0 40.0
30.0 30.0
20.0 20.0
10.0 10.0
0.0 0.0
200.0 250.0 300.0 350.0 400.0 [ nm ] 500.0

T Rf Substance Max. @
1 0.23 Rf AutoGenerated2 220 nm

AutoGenerated3 on all Tracks

100.0 100.0
[ AU ] [ AU ]
80.0 80.0
70.0 70.0
60.0 60.0
50.0 50.0
40.0 40.0
30.0 30.0
20.0 20.0
10.0 10.0
0.0 0.0
200.0 250.0 300.0 350.0 400.0 [ nm ] 500.0

T Rf Substance Max. @
1 -0.02 Rf AutoGenerated3 220 nm

AutoGenerated4 on all Tracks

100.0 100.0
[ AU ] [ AU ]
80.0 80.0
70.0 70.0
60.0 60.0
50.0 50.0
40.0 40.0
30.0 30.0
20.0 20.0
10.0 10.0
0.0 0.0
200.0 250.0 300.0 350.0 400.0 [ nm ] 500.0

T Rf Substance Max. @
1 0.11 Rf AutoGenerated4 218 nm

Evaluation results

Evaluation Sequence
Track Track type Vial Sample ID
1 Sample 1

User : Biologi Farmasi UGM Approved :. . . . . . . . . . . . . . . . . . . SN 1410W025, V1.4.3

Wednesday, October 08, 2014 10:10:06 AM Report ID : 07DE0A08040A0914 Page 4 of 5
 winCATS Planar Chromatography Manager

Table of substances
Position Tracks
Substance MD mm 1
AutoGenerated1 37.4 A
AutoGenerated2 28.0 A
AutoGenerated3 8.3 A
AutoGenerated4 18.5 A

User : Biologi Farmasi UGM Approved :. . . . . . . . . . . . . . . . . . . SN 1410W025, V1.4.3

Wednesday, October 08, 2014 10:10:06 AM Report ID : 07DE0A08040A0914 Page 5 of 5