Professional Documents
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3 FUNCTIONAL DESCRIPTION
3.1 Block Functions 3-1
3.2 Main Board Functions 3-1/3
3.3 Key and Indication Board 3-4
4 DIAGRAMS
For units with 120V mains voltage
4.1 Block Diagram 4-1
4.2 Wiring Diagrams 4-2/3
4.3 Wiring Connection Diagrams 4-4/6
For units with 100V mains voltage
4.4 Block Diagram 4-7
4.5 Wiring Diagrams 4-8/9
4.6 Wiring Connection Diagrams 4-10/12
For units with 100V or 120V mains voltage
4.7 Main Board, Component Plan, Wiring Diagram 4-13/19
7 CALIBRATION PROCEDURE
8 TECHNICAL BULLETINS
Edition: 01
17.11.02 MH
MICROLITRE CENTRIFUGES
HERAEUS® BIOFUGE® PICO AND BIOFUGE® FRESCO
Compact, fast and quiet
The microlitre centrifuges Typical Applications
in the Biofuge® family ● Pelleting DNA and RNA
are specially made to handle ● Pelleting of PCR amplified
biological separation tasks. nucleic acids
Whether non-refrigerated ● Antibody and protein
(Biofuge® pico) or precipitates
refrigerated (Biofuge® fresco), ● Enzymatic tests
these centrifuges are ● Removing cell debris from
indispensable in the modern cell homogenates
biological laboratory. ● Bacterial and yeast cell
Heraeus® centrifuges meet fractionation
all current European
Community guidelines and
are CE labelled.
Biofuge® fresco
● The smallest refrigerated
centrifuge for microlitre tubes
● Ultra-quiet standstill cooling
● Powerful CFC-free
refrigeration unit
● Max. capacity:
24 x 1.5/2 ml or
4 PCR-strips or
Biofuge® pico
32 individual PCR-tubes
● Incredibly small microlitre
● Max. speed: 13,000 rpm
centrifuge
● Max. RCF: 16,060 x g
● Short braking and
● Aerosol tight “Easy-Twist-On” lid
acceleration times
● Ergonomic design
● Max. capacity:
24 x 1.5/2 ml or
4 PCR-strips or
32 individual PCR-tubes
● Max. speed: 13,000 rpm
● Max. RCF: 16,060 x g
● Aerosol tight “Easy-Twist-On” lid
BIOFUGE® PICO AND FRESCO
Compact design High performance Ergonomic design
Create space in your labora- The brushless induction motor The smooth design of the
tory. Take advantage of the runs quietly. In 15 seconds casing and the modern
23 x 33 cm footprint of the or less, the rotor accelerates keypad control are easy-to-
small Biofuge® pico, which can to 16,000 x g, which is clean. The straightforward
be integrated into any work- sufficient for all standard arrangement of the keys,
place. The minimum height of applications. A “quick run” key with icon labels and the
the pico casing makes it easier is used for short processing EASYcontrol™ microprocessor
to load the rotor. runs. controller, make it simple
to operate.
The refrigerated Biofuge® fresco, The high-powered refrigeration
with a footprint of 29 x 45 cm, unit of the Biofuge® fresco One-touch lid opening allows
is scarcely larger than many with stand by cooling ensures for easy access to the rotor.
non-refrigerated centrifuges. temperatures beneath 0 °C
even at maximum speed.
MIKROLITRE ROTOR INCL. EASY-TWIST-ON AEROSOL TIGHT LID ORDER NO. 75003328
Internet http://www.kendro.com
Z pico 5e
Registered to ISO 9001. Kendro Laboratory Products meet or exceed stringent quality and product safety standards. ©2002 Kendro Laboratory Products. All Rights Reserved.
Printed in Germany 2C 09/02 4t Druckerei
Heraeus Pico Fresco Brochure 18/11/02 11:59 am Page 3
Benchmarks for
Size and Value.
Pico and Fresco
Microcentrifuges
SORVALL
®
Heraeus Pico Fresco Brochure 18/11/02 11:59 am Page 4
Typical applications
• Pelleting DNA and RNA
• Pelleting of PCR-amplified nucleic acids
• Antibody and protein precipitates
• Enzymatic tests
• Removing cell debris from cell homogenates
• Bacterial and yeast cell fractionation
Heraeus Pico Fresco Brochure 18/11/02 11:59 am Page 5
Compact
Choice of rotors
Microliter and PCR strip rotors for the SORVALL® Pico and Fresco
microcentrifuges
are equipped with biocontainment lids for use with infectious or
hazardous samples. The aerosol-tight, Easy-Twist-On lid is
exceptionally easy to use.
for Life Science
Compact design
Create space in your laboratory. Take advantage of the 233 x 335 mm
(9 x 13 in) footprint of the little Pico, which can be integrated
into any workplace. The low height of the Pico casing makes it
easier to load the rotor. The refrigerated Fresco, with a footprint
of 290 x 450 mm (11 x 18 in), is smaller than many non-refrigerated
centrifuges.
Stylish, ergonomic design
Whisper-quiet The smooth design of the casing and the modern keypad
The Pico and Fresco are valuable aids in the small biological controller are both easy to clean and pleasing to the eye.
laboratory, where noise can be an issue. Their designs take full The straightforward arrangement of the keys, with icon labels
advantage of recent discoveries in aerodynamics, and ensure and the EASYcontrol™ microprocessor controller, make operation
whisper-quiet operation even at maximum speed. straightforward. One-touch lid opening allows easy access to the
rotor chamber.
High performance
The brushless induction motors of the Pico and Fresco are extremely Ecological refrigeration
efficient. In 15 seconds or less, they accelerate the microliter rotor to The refrigerated Fresco uses a CFC-free refrigerant.
its maximum RCF of 16,000 x g, which is sufficient for all standard The powerful refrigeration unit, with standstill cooling, can maintain
applications. A “quick run” key is used for short processing runs. constant temperatures below 0°C even at maximum speed.
Specifications
SORVALL® Pico SORVALL Fresco
Max. RCF (x g) 16,060 16,060
Max. speed (rpm) 13,000 adjustable in 100 rpm increments 13,000 adjustable in 100 rpm increments
Min. speed (rpm) 2,000 2,000
Control Microprocessor-controlled via EASYcontrol ™
Microprocessor-controlled via EASYcontrol™
Drive Frequency-controlled brushless induction drive Frequency-controlled brushless induction drive
Noise level at max. speed < 55 dB (A) < 55 dB (A)
standstill cooling < 45 dB (A)
Run time 1 - 99 min, continuous operation, quick run 1 - 99 min, continuous operation, quick run
Program memory The most recently entered data remains in the memory The most recently entered data remains in the memory
Safety features Lid locks and interlocks Lid locks and interlocks
Temperature range - -9 to +40°C
Min. temp. at max. speed - 0°C (at room temp. = 25°C)
Dimensions (H x W x D) 8.1 x 9.2 x 13.2 in (206 x 233 x 335 mm) 12.0 x 11.4 x 17.7 in (305 x 290 x 450 mm)
Weight (without rotor) 21.3 lb (9.7 kg) 59.4 lb (27 kg)
Power consumption 150 W 270 W
Catalog Numbers
110-120 V/60 Hz 230 V/50-60 Hz
SORVALL
®
®
Quality Products – Lifetime Care
Registered to ISO 9001. Kendro Laboratory Products meet or exceed stringent quality and product safety standards Printed in U.S.A. (11/02) S00239
©2002 Kendro Laboratory Products. All Rights Reserved.
Maintenance
2 Service
2.1 Servicing Schedule (yearly procedure recommended)
2.1.1 Maintenance Routine without Dismantling the Centrifuge
2.1.1.1 Electrical Installation and Safety
· switch OFF the centrifuge and disconnect the unit from power, check voltage supply and mains
fusing (15 Amps, slow blow characteristic)
· check condition of plug and wall socket - (let) replace defective parts
· check cord condition and fixing / connection - replace or refit it
· check condition of instrument socket and replace it in case of bad contacts
2.1.1.2 Location and Mechanical Installation
· check the base (ground, table, lorry with lockable wheels etc.) for resonance-free and stable
conditions
· check for a well ventilated place and sufficient distances to walls or adjacent equipment, without
exposition to direct sunlight
· check the leveling of the centrifuge drive with use of a spirit level
2.1.1.3 Lid Locking Mechanism and Safety Device
· connect the centrifuge to power and switch ON
· check for easy lid closing and self-acting lid opening - if in disorder, readjust lid's swivel hinge
and/or torsion spring
· check the central rubber gasket for lid sealing and replace it, if damaged
· for checking of the safety circuit: start the centrifuge let it shortly run and stop it, the lid must not be
opened as long as the message "end" will be indicated - if safety circuit is out of function, replace
main board
2.1.1.4 Cleanliness of Spin Chamber and Motor Casing
· open the lid and remove the rotor (for loosening turn socket wrench in arrow direction - see rim of
rotor chamber, push 2 screw drivers in opposite holes and pull the rotor vertically out)
· clean the spin chamber with a dry and absorbent cloth (remove all dust and moisture - see also
section for Cleaning)
· check the correct seat and condition of the motor cover and take care of the cleanliness of the
annular slot around the motor shaft: penetrating fluids can damage the upper motor bearing,
remove fluids with an injector and/or absorbent paper
2.1.1.5 Rotor and Accessories Condition and Sealing
· check the condition of rotor and accessory parts (especially all supporting or stressed partitions):
the rotor and/or accessory parts must not be used any longer, if there are visible traces of
mechanical damage or rust
· check the condition of rotor and/or accessory sealing and replace them in case of malfunction
Trouble Shooting
Error Possible Error
Error Cause Corrective Procedure
Indication Source
Forbidden!
Lid was opened
Lid opening device close lid immediately, turn power OFF/ON, wait for
manually during
must only be used at termination of br phase until end message appears
run
standstill
Defective micro check leads and connectors to micro switch, in case
„LId“ switch or leads or of a faulty micro switch, replace lid lock device
message protection circuit connectors to micro completely
appears in (15V) for lid and switch are let motor cool down, then check temperature switch
speed motor interrupted and leads with Ohmmeter
display temperature Motor over- check air cooling, circulation
control was temperature switch
interrupted has tripped (no
during run sufficient air flow or check solderless connections XM1-3 and motor
motor has run on 2 windings, replace defective parts
phases only)
15V supply micro switch check leads and micro switch
„OPEn“
circuit is
message in motor over- check motor over-temp. switch and leads for
interrupted at
display temperature switch continuity, replace faulty parts
standstill
“br“ short interruption of
rotor comes to Wait for rotor standstill (approx. 75 sec) and re-start
message mains supply
standstill
appears in bad contact of
without braking Check plug contacts of supply line and replace
speed instrument supply
force defective parts if necessary
display line
check for easy movement, remove any jamming
rotor is jammed
rotor didn't turn objects
motor is jammed remove drive, replace faulty parts
E-00 connection: drive check terminal and lead connections, replace faulty
flashes main board parts
motor didn't start check resistance of motor windings, replace faulty
defective drive
parts
faulty main board replace main board
faulty faulty temperature
check plug connection and resistance of PT 100,
temperature sensor (PT100) or
replace faulty sensor
measuring leads
E-04 test: PT100
set the temperature to 20°C and watch the display:
flashes substitution by
defective amplifier or 100W ® (0±2)°C
resistors,
converter 120W ® (50±2)°C
indication after
power ON if not replace the main board
chamber over compressor didn't check leads and voltage supply on main board and
temperature work compressor, replace defective parts
indication value
>50°C search for leakage, evacuate and refill with new
E-04 loss of refrigerant
refrigerant
flashes
chamber under
compressor didn't check compressor relay and relay driving, replace
temperature
cut off defective parts
indication <-10°C
Trouble Shooting
Error Possible Error
Error Cause Corrective Procedure
Indication Source
E-10 checksum error NV-RAM wrong or not
insert the correct NV-RAM
flashes of NV-RAM initialised
sporadic: strong
if error returned very often screen the disturbance
electromagnetic
error during data source or operate the centrifuge on another place
E-11 disturbance
exchange from
flashes
NV-RAM continuously: error
replace defective main board
on main board
sporadic: strong
too much if error returned very often screen the disturbance
electromagnetic
E-23 tolerances during source or operate the centrifuge on another place
disturbance
flashes temperature
calibration continuously: error
replace defective main board
on main board
lid solenoid
20W resistance , not activated
terminal XC
lid switches
15V DC voltage drop by open lid
plug XA
Over temperature
switch Motor 15V DC voltage drop by closed lid and disconnect one pole of terminal XB
terminal XB
Temp. Sensor voltage drop
95mV
plug XU chamber temperature 20°C (PT100: » 108W)
2.4.1 Purpose
The torsion spring offers the user a comfortable support when closing and opening the centrifuge lid.
When the "open lid" key is pressed, the lid lock is released on both sides and the lid bursts open and
remains in a 20° position. Up to an angle of 45° the lid remains stable in any chosen position, beyond
45° it automatically opens to full extent.
2.4.2 Mounting
The torsion spring assembly contains the following parts:
· torsion spring
· 2 plain bearing bushes (for shaft guidance)
· 2 bushing nipples (for leg catch)
· 2 set screws (aid for fixing legs)
· dowel pin (for hooking the torsion spring onto the shaft)
· tube of Loctite 221 (for fastening screws)
· tube of Molykote TP42 (for lubrication of the torsion spring)
Preparation:
The defective torsion spring has been removed as described in Section 5, the old plain bearing bushes
and bushing nipples have been removed.
1. Press the new plain bearing bush from outside into the right-hand bore of the chassis.
2. Slip on both bushing nipples up to the head of the hexagon bolt M6x12. Fix the screws again to the
chassis and secure with Loctite 221.
3. Push the old dowel pin carefully from the shaft using a pin punch, then push the new dowel pin on
using a plane surface (e.g. a wooden panel).
2.4.3 Adjustment
Preassembly and alignment of the legs:
8. Push both legs onto the ends of the shaft such that the bores are horizontally oriented and the leg
ends point forward.
9. Screw the M8 nuts loosely onto each of the shaft ends to position the legs.
10. Screw one dowel pin each tightly into the rear threaded bores, thereby fixing them with the bore in
the chassis.
2.5.2 Mounting / Adjustment of the drive with help of the rotor #3327/3328
Fasten rubber buffers to the transverse crosshead using nuts and washers.
· Place transverse crosshead inside the chassis and fasten with screws such that the screws are
aligned with the upper ends of the long holes.
· Connect the motor to the rubber buffers using the screws and locking rings.
ATTENTION - WARNING!
The electrical and electronic components must not be cleaned with moist detergents!
For Cleaning the centrifuge housing or its accessories see Operating Instructions section 1
(maintenance and care).
· Electronic components
Clean dusty components carefully with a dry and soft brush and remove loose dust with a
vacuum cleaner.
· Fans
Scratch off carefully with a knife or similar tool the crusted dirt from the fans’ blades. Resulting
grooves or marks must be removed subsequently with a fine abrasive cloth. Loose dirt is to
remove with an absorbent cloth or vacuum cleaner.
· Vent holes
Remove dirt from the vent grid behind the front panel by using a brush and vacuum cleaner.
· Condensers of cooling plant
The louvers between the condensers’ heat air deflectors have to be vacuum cleaned. In case of
persistent dirt the unit must be carried to an appropriate place where the condensers can be
cleaned with compressed air from inside to outside. Bent heat air deflectors have to be realigned
correctly.
ATTENTION!
A final electrical safety check must be performed after each maintenance and/or repair!
0,022µF V
±5% AC
Specifaction for the meter
500W - TRMS, DC - 5kHz or more
±1% - Input resistance > 1MW
- Tolerance 5% or better
- Crest Factor 5 or better
L (N)
Körperstrom:
N (L) accessible current: I [mA] = U [mV] / 500:
PE Umax = 1750mV º Imax = 3,5mA
3 Functional Description
The BIOFUGE fresco is a microprocessor controlled laboratory tabletop centrifuge with induction
drive motor and refrigerator. Following boards and components are located inside of the unit (see
block diagram section 4 page 4-1):
· Two poles mains switch is mounted in the bottom of the front panel's right side
· Main board with microprocessor part and power electronics, 2 fuses (4AT) are serving for board
protection
· Key and indication board (EASYCONTROL programming with temperature display)
· Three phase induction drive motor with an integrated thermal over temperature switch (cut-out at
140°C)
· Temperature sensor PT100
· Compressor with starting relay (inside terminal box) and starting capacitor
· Two lid lock assemblies with solenoid and integrated micro switches (mechanical bolt keeper,
magnetically dislocking)
· Brake resistor mounted on main board
· Two fans (parallel connected) for cooling of condenser, drive, compressor and electronics
The main board is mounted on the rear side of the front panel. The components on main board are
arranged in following groups (see also wiring diagram page 4-2 and 4-3).
· Board fusing (2 x 4 Amps. slow blow feature)
· Noise Filter for suppression of radio interference, caused by the fast switching power electronics
(9,9 kHz), in accordance with the German Regulation VDE 871, grade B
· Power pack for low voltage supply of microprocessor part (potential separated by transformer) and
power electronics (mains potential)
· Triac control circuit for both parallel connected fans
· Triac control circuit for both parallel connected lid solenoids ( DC supply via bridge rectifier)
· Triac control circuit for compressor relay
· Bridge rectifier for DC intermediate circuit supplying brake control path and frequency converter
· Microprocessor part with Controller (central processing unit) and ASIC80 (specific integrated circuit
for controlling of centrifuge functions)
The power pack consists of a transformer, bridge rectifier and voltage regulator.
· U1 = 5 V supplies central processor, key and indication board and temperature measuring
circuit
reference potential A1: connected to protective conductor (GND)
Brake Path
Electrical power is fed back into the intermediate circuit during motor deceleration (motor acts as
generator). This braking power is transformed into heat by two big brake resistors (parallel connected)
on board so that the intermediate circuit voltage does not rise to an excessive level (see section 2 -
test points on main board). The brake resistors are switched into the intermediate circuit by a fast
switching transistor (pulse width modulation). This transistor is voltage dependant controlled by a self-
acting stage (closed loop).
Frequency Converter
The motor is 3-phased provided with chopped direct voltage blocks. These blocks are variable in
frequency and pulse width modulation and are dephased to 120° between the motor windings.
Beginning with low frequency and small pulse width chopped blocks, both parameters will be
increased up to the operators set speed. By this variation the acceleration power of the drive motor
can be effected. For deceleration the frequency and pulse width parameters will be reduced. The
frequency converter is protected against over current, over temperature and over voltage.
· CPU: 097 8 xx (first displayed No. in speed, time and temperature field)
· RAM: 415 8 xx (second displayed No. in speed, time and temperature field)
The second parts of these No.'s (xx displayed in the temperature field) will be upgraded if necessary.
The ROM with control program is integrated in the CPU. The most important operating parameters
(e.g. the maximum data or the last operator settings) are stored in the non volatile (NV) -RAM.
The CPU co-operates with the ASIC 80 component via the 8 bit data bus and some control lines. The
ASIC 80 is a specially designed integrated circuit for the driving of our centrifuges (with induction
motors), containing additional functions for temperature measuring and electronics protection.
instrument plug
x1000
S1 BIOFUGE
mains switch
key and indication board XF
XF
XN
main board
NV-RAM
brake resistor CPU
XA XC XB XM XU XK XG
F3
PT
S3 S1 S4 S2 3~ V1 100
Y1 Y2
M1 M2 M3 M4
lid solenoid lid switch lid solenoid lid switch overtemp. switch motor temp. sensor fan for compressor, condenser compressor
legend
screwing connection plug connection
Edition: 01 Block Diagram
16.12.02 He 4-1 BIOFUGE fresco
SCHEMATIC DIAGRAMS
For units with 120V mains voltage
A1=GND
XN 2
XPE
"E-07"
"E-08"
J
K1 5 autom. brake control
PTC "E-08"
"E-07"
R1 220
brake resistor
XG/1 XG/2 XV/1 XV/2 XB/1 XB/2 XA/1 XA/2 XC/1 XC/2
M2
"E-17"
14 10
S1..S4
Y1
"E-07" "LId"
Î
PE chassis PE compressor CS "E-17"
PE condensor fan M2 11 M3 Y2
PE motor
"E-07"
3
compressor with starting relay fan for condenser
4
"E-” “ 0"
"LId" i dyn
"OP"
Û “E-” “ 8”
3 F
R44 idyn 15V “LId”... "E-7"
A2 U
2 idyn “E-” “ 0”
current control res. UD-*
"E-” “ 0"
3 physical separation
F3 V1
overtemperature switch
J
4 temperature sensor
"LId" M1
PT100
Motor
motor
Edition: 01 Wiring Diagram
16.12.02 He 4-3 BIOFUGE fresco
SCHEMATIC DIAGRAMS
For units with 120V mains voltage
mounting of unit ground, right side in front mounting of unit ground, left side in front
PE - mains ground
PE - armored housing
starting capacitor
terminal: 11 u. 13
mains - L
10 11
starting capacitor
120mm
mains - N 12 13 5x 400 mm
14
PE terminal box
XB1
overtemp. switch
PE XB2
compressor wiring
XM1
XM2 motor connections
+ XM3
XG/N +
XG/L PE - motor, 300mm
to amored chamber in front
XK/L
fans
wiring fans
XK/N
PE - Lüfter
PE - fans
M2 M3
PE - main board
unit ground, left side in front
1x PE - terminal 1 pole
1x screw M4x10mm
1x PE - terminal 1 pole 2x fan washer 4,3mm
1x screw M4x10mm
2x fan washer 4,3mm
bottom plate
unit socket
clip 6,5mm
fan 3, right side
cable conduction by clips
front of unit
KM
clip 6,5mm
fan 2, left side
adhesive clip
plug designation length from clip
XA lid switches S1 + S2 125 mm
overtemp. switch
drive
XC lid solenoid’s Y1 + Y2 150 mm
XG compressor 300 mm
*
XK fans M2 + M3 210 mm
coil
switch
PE - fan 2 PE-fan 3
*
fan M2 * fan M3
S0
temperature sensor lid lock, switches drive fans lid lock, coil compressor mains input
instrument plug
x1000
BIOFUGE
S1
mains switch
key and indication board XF
100V
auto transformer
100/115V XF
115V
XN
main board
NV-RAM
brake resistor CPU
XA XC XB XM XU XK XG
F3
PT
S3 S1 S4 S2 3~ V1 100
Y1 Y2
M1 M2 M3 M4
lid solenoid lid switch lid solenoid lid switch overtemp. switch motor temp. sensor fan for compressor, condenser compressor
legend
screwing connection plug connection
Edition: 01 Block Diagram
16.12.02 He 4-7 BIOFUGE fresco
SCHEMATIC DIAGRAMS
For units with 100V mains voltage
A1=GND
T0 XN 2
XPE
"E-07"
"E-08"
J
K1 5 autom. brake control
PTC "E-08"
"E-07"
R1 220
brake resistor
XG/1 XG/2 XV/1 XV/2 XB/1 XB/2 XA/1 XA/2 XC/1 XC/2
M2
"E-17"
14 10
S1..S4
Y1
"E-07" "LId"
Î
PE chassis PE compressor CS "E-17"
PE condensor fan M2 11 M3 Y2
PE motor
"E-07"
3
compressor with starting relay fan for condenser
4
"E-” “ 0"
"LId" i dyn
"OP"
Û “E-” “ 8”
3 F
R44 idyn 15V “LId”... "E-7"
A2 U
2 idyn “E-” “ 0”
current control res. UD-*
"E-” “ 0"
3 physical separation
F3 V1
overtemperature switch
J
4 temperature sensor
"LId" M1
PT100
Motor
motor
Edition: 01 Wiring Diagram
16.12.02 He 4-9 BIOFUGE fresco
SCHEMATIC DIAGRAMS
For units with 100V mains voltage
mounting of unit ground, right side in front mounting of unit ground, left side in front
PE - mains ground
PE - armored housing
starting capacitor
terminal: 11 u. 13
mains - L
10 11
starting capacitor
120mm
mains - N 12 13 5x 400 mm
14
PE terminal box
XB1
overtemp. switch
PE XB2
compressor wiring
XM1
XM2 motor connections
+ XM3
XG/N +
XG/L PE - motor, 300mm
to amored chamber in front
XK/L
fans
wiring fans
XK/N
PE - Lüfter
PE - fans
M2 M3
PE - main board
unit ground, left side in front
1x PE - terminal 1 pole
1x screw M4x10mm
1x PE - terminal 1 pole 2x fan washer 4,3mm
1x screw M4x10mm
2x fan washer 4,3mm
bottom plate
unit socket
clip 6,5mm
fan 3, right side
cable conduction by clips
front of unit
KM
clip 6,5mm
fan 2, left side
adhesive clip
plug designation length from clip
auto transformer
XA lid switches S1 + S2 125 mm BV30326
Prim.100V Sek.115V
XB1/2 overtemperatur switch 140 mm
overtemp. switch
XC lid solenoid’s Y1 + Y2 150 mm
drive
XG compressor 300 mm
XK fans M2 + M3 210 mm *
auto transformer
coil
100/115V
switch
PE - fan 2 PE-fan 3
*
fan M2 * fan M3
S0
temperature sensor lid lock, switches drive fans lid lock, coil compressor mains input
XU
Stecker
Temperaturfühler
plug
temperature sensor
XD
Anschluss
Service
XC plug
Anschluss service
Deckelspule
terminal
lid solenoid
XK
Anschluss
Lüfter
terminal
fans
XG
Anschluss
Kühlmaschine
terminal
compressor
XN
Anschluss XA
Netz Stecker
terminal Deckelschalter
mains plug
lid lock, switches
+15 +15
U-* U-*
U+
DECKELSCHLOSS DECKELSCHLOSS
TEMP_1 TEMP_1 KUEHLUNG KUEHLUNG
LUEFTER LUEFTER
R16
2R5
NTC
EMI Filter
Funkentstoerfilter D9 R18
Power Connection Hilfszwischenkreis ZK2
C16
XN F1 2n2 1N4007 470R
1 L1 Y SMD
L 1 3 D4 BR1
2 SI 8AT 1 2 U+
N 6.3x32 UL R17 C15
120k 0u6 P600K BR_POWER
X C13
470u
2 4 C19 R14
XPE1 200V
220n 56k
PE 2x5.6mH 400V
2A
U0
C14 DC intermediate circuit
470u
R15 200V
56k
D5
U-
C9 C10
P600K 2n2 2n2
Y Y
not placed
V6
V1
T1 780M05C
SM4007
SMD 2 VI VO 3 VCC
120V G
REL120V HL1-HP-AC120 V7 V5 N
SM4007 D
T120V/9V SM4007
BV28969 GASS SMD SMD C21
V8 C11 1 10u C12
1 C20 100n 100n
1000u SMD C71 SMD
Z1 25V 10u
0u1/100R 2 3
K1a SM4007 SMD SMD 25V TA
compressor 1 4 GND
XG
2
2
R21 to drive compressor
1 KUEHLUNG
6 1
D6 330R
V2 R19 S21ME4 SMD
R28
100R T1213 47R
4 2
SMD
V50 SMD
GND
lid solenoid C17 R20
XC 2n2 1k
SM4007 Y SMD
2 SMD
to drive lid lock
1 V62 V61 DECKELSCHLOSS
SM4007 SM4007 6 1
SMD SMD D7
V63 1 V3 R22 S21ME4
SM4007 T1213 47R
4 2
SMD Z2 SMD
0u1/100R GND
fans R23
XK 1k
2 SMD
2 2R6 PTC
R46 R27 to drive fans
1 LUEFTER
6 1
D8 330R
V4 R25 S21ME4 SMD
R29
100R T1213 47R
4 2
SMD
SMD
GND
C18 R26
2n2 1k
Y SMD
C2
auto calibration 100n
SMD
R2
22k
SMD
VCC
8 D1A R4
TLC272 270k
3 SMD VCC VDD
SMD
R1 1
2
C5
1k
SMD 4
1n
D2 D1B
SMD
74HC4052 TLC272
R12 GND (74HCT4052)
SMD SMD
120R R3
temperature sensor SMD 0.1% 12 13 6
X0 X 22k C3 C4
Pt100 14 7
X1 SMD 100n 100n
15 3 5 VSS GND VEE
XU1 11
X2 Y SMD SMD
X3
1
1
2 1
2 Y0
5
R13 Y1
2 R5
100R Y2
4 100K
SMD 0.1% Y3
6 SMD
GND INH
10
TEMP_1 A
9 Voltage to Frequency Converter
TEMP_0 B VCC nicht bestueckt
not placed
R9
Temp_1 Temp_0 X,Y
390R A B D3
SMD 0 0 100R LM331 R11 R10
8 C8 C73
1 0 120R C6 39k 10k 10u 10u
1u SMD 35V Ta V SMD SMD SMD 25V TA
1 1 PT100 7
S
5
COMP IN R/C
GND
C7 GND GND
1u SMD 35V Ta R
1 E
CURR IN F
3
F OUT TEMPERATUR
6 G C
THRES N U temperature signal
R6 D R
100K 4 2
SMD C1
GND 220n
Fo
R7
12k
SMD
GND
R8
2k7
SMD
GND
XA1
U+
XA
motor overtempemperature lid lock, switches
2 1 not placed
switch
XB R91
2 1 220R SMD
1 2
U0 +15
R98 XB1 F2 VCC
10k E11T11G80
11W R24 R101 118 GRAD
470R 470R R92
9W 9W 22k 1 3
SMD D34
SFH6106-3
V51 C76 2 4 SMD
UEBERSTROM
ZMM15 100u
25V SMD Brems- V52 overcurrent signal
SMD
Widerstand P6KE440A R96
12k
R87 Brake C63 C75 SMD
R99 Resistor 100n 10u
C64 D33
1M SMD SMD 25V TA
100n
2M2 SFH6106-3
SMD C66 C74
7 SMD SMD C60
100n 10u 10u
3 R107 V49 R97 SMD SMD 25V TA
6 BUZ215 R93
1 3 12k
2
R95 D32 C68 SMD
15R
68k TL081 DECKELAUF
SMD 470p 2k2 SMD
R88 SMD SMD
4 1 5 2 4 SMD 1 3 lid open signal
10k R94 D35
SMD 1% V47
V48 SFH6106-3
C65 BC817
GND GND BC817 2 4 SMD
47p 2k2 SMD
SMD C61 C62
10n 10n GND
SMD SMD
U- U-*
R100 0R18 2W
R89
10k
SMD 1%
/UEBERSPANNUNG
DC intermediate circuit excess voltage signal
voltage control
R90 relative excess voltage recognition absolute excess voltage recognition over current recognition
1M
active, if the motor put back power active, if the brake resistor is faulty or if
ZK2 to the intermediate circuit the mains voltage is too high
VCC
C58 C46 C45 C38
10u 100n 100n 100n
V27
SMD SMD SMD R76 C54 BC807
not placed C73
100k 10u
10u SMD SMD 25V Ta C47
GND GND GND GND GND V28
SMD 25V TA BC807
R77 10n
INHIBIT SMD GND
100k R65 V44 R78
SMD U+
1 3
220R D18 LL4148 100k
SMD SFH6106-3 V46 2W
2 4 SMD ZMM12 R48 V21
V29 R64 SMD BUZ215
VCC 1 3 R58 15R
LL4148 D26A D26B 220R D19 C55 1M C48 SMD
R81 SMD SFH6106-3 10u V40 1n
1 2 3 4 2 4 SMD LL4148 SMD
PHASE1_O SMD
47k R66 SMD 25V TA
SMD C39 74HC14 74HC14 +15
220p SMD SMD 1 3
SMD 220R D20
GND SMD SFH6106-3
2 4 SMD R102 V22
V30 R67 BUZ215
VCC R59
1 3 1M 15R
LL4148 D26D D26C 220R D21 V39 C51 SMD
R82 SMD SFH6106-3 LL4148 1n
9 8 5 6 2 4 SMD SMD SMD
PHASE1_U
33k
SMD C40 74HC14 74HC14
220p SMD SMD
SMD R69 V43 R80
GND
1 3
220R D22 LL4148 100k
SMD SFH6106-3 V45 2W
2 4 SMD ZMM12 R103 V23 Motor
V31 R68 SMD BUZ215
motor
VCC 1 3 R60 15R XM1
LL4148 D26E D26F 220R D23 C56 1M C49 SMD
R83 SMD SFH6106-3 10u V38 1n 3
11 10 13 12 2 4 SMD LL4148 SMD
PHASE2_O SMD 2
47k R70 SMD 25V TA
SMD C41 74HC14 74HC14 1
220p SMD SMD 1 3
SMD 220R D24
GND SMD SFH6106-3
2 4 SMD R104 V24
V32 R71 BUZ215
VCC R61
1 3 1M 15R
LL4148 D27B D27A 220R D25 V37 C52 SMD
R84 SMD SFH6106-3 LL4148 1n
3 4 1 2 2 4 SMD SMD SMD
PHASE2_U
33k
SMD C42 74HC14 74HC14
220p SMD SMD
SMD R73 V41 R79
GND
1 3
220R D28 LL4148 100k
SMD SFH6106-3 V42 2W
2 4 SMD ZMM12 R105 V25
V33 R72 SMD BUZ215
VCC 1 3 R62 15R
LL4148 D27C D27F 220R D29 C57 1M C50 SMD
R85 SMD SFH6106-3 10u V36 1n
5 6 13 12 2 4 SMD LL4148 SMD
Phase3_O SMD
47k R74 SMD 25V TA
SMD C43 74HC14 74HC14
220p SMD SMD 1 3
SMD 220R D30
GND SMD SFH6106-3
2 4 SMD R106 V26
V34 R75 BUZ215
VCC R63
1 3 1M 15R
LL4148 D27D D27E 220R D31 V35 C53 SMD
R86 SMD SFH6106-3 LL4148 1n
9 8 11 10 2 4 SMD SMD SMD
Phase3_U U-*
33k
SMD C44 74HC14 74HC14
220p SMD SMD
SMD
GND
The index numbers stated in ( ) reappear within the spare part figures (block diagram, schematic
diagram of cooling plant) and the spare part lists.
Assembling instructions
· Push the gasket over the rim of the rotor chamber, starting with the gluing seam in front.
· Work from both sides to the rear, but do not lengthen the gasket by this way.
· While doing so press the sealing edge under the casing's rim.
· Fold the rim of the rubber sealing over the rotor chamber's opening, starting from front to rear
· Press the casing back again onto the bottom plate and assemble it analogously in reverse order.
· The lid gasket must be completely sit close on the rotor chamber wall!
· The rotor chamber must be sealed completely, when the lid is closed correctly!
For mounting and adjustment of the torsion spring you must follow the instructions in section 2.4,
please note also the drawing on page 6-4.
The microcontroller normally comes with the board. It could be possible that there will be a change
sometime necessary when the will be upgrades.
· Notice the old Controller's version number when powering on (first displayed No.).
· Depose the front panel as described in 5.4.1.
· ATTENTION - CMOS components! Discharge your body before handling! Notice the correct
Controller position (CPU) and pull out carefully. ATTENTION - do not damage the socket (use
cranked pliers)!
· Reinsert the new Controller chip correctly.
· Reassemble the device analogously in reverse order and check the displayed Controller's version
number.
Note! A new Controller version will include program changes (e.g. modifications of control functions)
which might be verified only by measuring performances.
There must not be used any kind of hoses, armatures and manifold gauges which are already
contaminated with CFC based refrigerants. Otherwise the compressor oil will become an
aggressive condition and will destroy the compressor in a short time.
The pinch valve must not be left on the line! Close the system hermetically (solder close) and fix the
service lines using cable ties with a little drop to let the oil run back after transportation.
Re-assemble the unit in reverse order and test the cooling system (see Servicing Schedule).
130
91
123
90
120
82 124
81 115
135
110 95
83
84 80
112
116 125
100
115
125
101
126
110
105
121
122
(50)
113
220
222
221
062
236 223
225
062 235 224
219
210 236
27.6 234 231
061
237 2.5
238 200
214 216 205 201
060
215 230
203 040
204 202 232 240
210 239
231
210 207
210 208
206 031
204 203 030
205
208
207
245
245 247
210
247
246
218
245
247
252
300 331
322
320
303
301
302
062 306
307
060
313
308
314 309
346
305 347
070 340
340
342
330 340 365
333 364
370
380
312
311 061 361
071 374
331 362
332 345 363 370
381
310 313
325 382
314 360
334
Edition: 01 Parts on Bottom plate
16.12.02 He 6-3 Biofuge fresco
BREAK DOWN DRAWINGS
15°
00
Molykote TP 42 (40)
(11)
M6x6 (135)
18. Close the lid and replace the rear cup screws on both sides by set screws.
14. Tighten screw M6x6 (135)
19. Screw lid off and mount housing. Reassemble lid as descripted above (13.)
20. Check opening angle
xx. see mounting and adjustment of the torsion spring, page 4-10/11
(xx) Index number of the spare part list
Figure B
position indicator
Figure A
Cover (221)
±0,5
Y=2.5
Support (222)
front side
figure C figure D
figure E figure F
not correct
Seite 1
Seite 2
Seite 3
Seite 1
Seite 2
Seite 3
Seite 1
Seite 2
Seite 3
Imbalance
o Centrifuge stable and level
Preliminary Checks o Centrifuge shuts off w/proper weight
o Clean inst. & condenser coils
o Rotor chamber (defrost) Safety test
o Inst. environment
o Accessible leakage current <3500µA
(level & clearance)
o Insulation resistance >10 Megaohm
o Earth Conductor Resistance <0.2 Ohm
Pre-run Checks
o Ground continuity
o Line voltage ________V AC
o Display segments on power-up
o Microprocessor Initialization (if required)
o Door interlock and latch
o Drive stop & Door unlatch (50 rpm)
o Compressor current draw
o Fan operation (condenser & compressor)
Mechanical
o Motor bearings
o Refrigerant leaks
Revised – 11/11/02 1 of 2
Kendro service Information
Comments:
Revised – 11/11/02 2 of 2
Calibration Certification Procedure
Biofuge fresco
Revised - 11/11/2002
1. General
2. Equipment Required
· Digital Multimeter
· Temperature Meter w/probe
· Stopwatch
· Photo Tachometer
· Safety Tester
· Biofuge fresco Service Manual
· Centrifuge Operating Manual
Note: Check calibration due date on all test equipment before starting certification.
3. Documentation Required
· Perform Preventive Maintenance checks as outlined in the Kendro Service Manual to ensure
the instrument is in good working order without performing any calibrations.
· Complete the Preventive Maintenance Checklist. (calibration data will be filled in after
Certification)
Calibration Certification
· SPEED
Install a rotor, set the speed control to 3000 rpm and start the centrifuge. When the speed has
stabilized, measure the actual speed with an external tach (photo tach on multimeter where speed
= frequency[no conversion]). Record measured speed and displayed speed in the speed section of
the certification form. Set the speed control to max. rpm and after stabilization, record measured
speed and displayed speed in the speed section of the certification form. Determine if the speeds
recorded meet the specifications on the form. If the specifications are met, proceed to the next
section. A recalibration of the instrument is not possible.
fresco_7b_e 1 of 2
Calibration Certification Procedure
Biofuge fresco
· TEMPERATURE
Place water into the rotor or bucket. Select a temperature of +4°C, maximum allowed speed of
the rotor and time of minimum 3 hours. After that record the displayed temperature, measured
temperature inside rotor or bucket after rotor has come to rest, in the temperature section of the
certification form. Repeat the test at 15°C. Record the values in the temperature section of the
certification form. Determine if the temperature values recorded meet the specifications on the
form. If the specifications are met, proceed to the next section. A recalibration of the instrument
is not possible. Initial the temperature section. To pre-cool the rotor select half maximum speed.
· TIME
Using a stopwatch, check the timer by setting a 10 minute run. Start the centrifuge and measure
from the time the start button is pushed until the timer switches to stop. Record the measured
time in the time section of the form. Determine if the time value recorded meets the specifications
in the form. If the specifications are met, proceed to the next section. A re-calibration of the
instrument is not possible. Initial the time section.
· SAFETY TEST
Using safety tester and connect the centrifuge to it. Measure Insulation resistance.. Select to Earth
Conductor resistance and touch with probe all mechanical part of the centrifuge. Resistance must
be <0.2 ohms at all points otherwise check for good grounding. Measure accessible current to
procedure in the service manual.
Documentation
fresco_7b_e 2 of 2
Biofuge
Calibration Certification Fresco
Page 1
Preventive Maintenance Checklist Complete Repair and Calibration Recertification
Performance Certification
SPEED
Standard Test Rotor Used:
Set Speed (rpm) Tolerance Displayed Measured Meets Spec. Measured Passed
Speed Speed (y/n) Speed after (Initials)
(rpm) (rpm) Cal.(rpm)
13000 +/-5%
+/-5%
Custom Test Rotor Used:
+/-5%
+/-5%
TEMPERATURE
Standard Test Rotor Used:
Calibration Tolerance Displayed Measured Meets Measured Passed
Temp. (K) Temp. Temp. Spec. Temp.after (Initials)
(°C) (°C) (°C) (y/n) Cal. (°C)
+4 +/- 2
+15 +/- 2
Custom Test Rotor Used:
+/- 2
+/- 2
+/- 2
TIME
Standard Test Rotor Used:
Timer Set Tolerance Measured Meets Measured Passed
(min.) (sec.) Time Spec. Time after (Initials)
(m/s) (y/n) Cal (m/s)
10 +/- 30
Custom Test Rotor Used:
+/- 30
+/- 30
+/- 30
TEST EQUIPMENT
Type Model Serial Number Date of Next Calibration
Stopwatch
Temperature Meter
Photo Tachometer
Safety Tester
SAFETY TEST
Insulations resistance Earth Conductor Resistance Accessible Current
>10 Megaohm <0.2 Ohm <3500µA
Revised 12/1/01
Certified By:_____________________________ Reviewed By:________________________________ Expiration Date ______
I:\TS\Service_Manual\Biofuge_fresco\230V\Fresco gruen\Fresco_7c.doc