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ISOLATION OF PHYTOCHEMICALS
AND BIOLOGICAL ACTIVITY STUDIES
on
The Bark of Thespesia populnea Linn-

SYNOPSIS FOR

M.PHARM DISSERTATION

SUBMITTED TO

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES

By

Prasoon Kumar Saxena

Department of Pharmacognosy

THE OXFORD COLLEGE OF PHARMACY

BANGALORE-78
 RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,
KARNATAKA, BANGALORE.
ANNEXURE-II

PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION

1. Name of the candidate and Prasoon kumar saxena

Address The Oxford College of Pharmacy,
J.P. Nagar, Ist Phase,
Bangalore-560078.

PERMANENT ADDRESS:
S/o Shri Naresh kumar saxena,
Shiv puram Lane no-4,
Dist- Budaun, U.P.
Pincode-243601.

2. Name of the Institution

The Oxford College of Pharmacy,
J.P.Nagar, Ist Phase,
Bangalore-560078.

3. Course of study and subject M.Pharma, Pharmacognosy

4. Date of Admission 11th May 2007

5. Title of the Topic ISOLATION OF PHYTOCHEMICALS

AND BIOLOGICAL ACTIVITY STUDIES
ON
The Bark of Thespesia populnea Linn.
6. Brief resume of the intended work:

6.1- Need for the study:

Thespesia populnea is also called as Portia tree, belonging to family

Malvaceae. It is widely distributed in India and Burma.

The plant contains glycosides such as quercetin, gossypol, and β-sitosterol 1.

sesquiterpene ie. Thespesenone and dehydrooxoperezinone-6-methyl ether were isolated

from the red hard wood of Thespesia populnea2. Alanine, arginine, methionine and

tryptophan were isolated from seed of thespesia populnea3. Thespesia populnea also contain

lupenone, lupeol4.

The ethanolic extract of Thespesia populnea bark reported for anti-

inflammatory and analgesic activity5. Aqueous and methanolic extract of the Thespesia

populnea showed antioxidant activity against carbon tetrachloride induced liver injury in

rats6. Thespesia populnea also recommended for anti-fertility activity7, wound healing8,

dermatitis9, anti-bacterial and anti-fungal10, and hepatoprotective11 activity.

The more emphasis has been given to the pharmacological aspects of the

plant but a very less work has been done on the phytochemical investigation.

The aim of the present study is to carry out the isolation of

phytoconstituents and biological activity of the extract / isolated compounds.

6.2 – Review of Literature:

1. Glycoside was reported from the Thespesia populnea flower1.

2. Sesquiterpene was isolated from the red hard wood of Thespesia populnea2.

3. Amino acids were isolated from the Thespesia populnea seed3.

4. Lupenone and lupeol were isolated from the plant4.

5. The ethanolic extract of Thespesia populnea bark showed anti-inflammatory and

analgesic activity5.

6. Antioxident activity was reported from the aqueous and methanolic extract of

Thespesia populnea bark6.

7. The antifertility activity of gossypol, a phytocostituent was isolated from Thespesia

populnea bark7.

8. The aqueous extract of Thespesia populnea fruit showed wound healing activity8.

9. The plant extract was reported as dermatitis9.

10. Antibacterial and antifungal activity was reported in alcoholic and chloroform extract of

Thespesia populnea10.

11 .Methanolic and aqueous extract of Thespesia populnea bark showed hepatoprotective

activity11.
 6.3 - Objective of the Study:

 Collection of the authenticated plant material (i.e. bark of Thespesia

populnea) from Dr. Harish Botanist. Alva’s education foundation ( R ).
Alva’s Health centre complex Moodbidri – 574227. D.K.

 Preparation of the extract of the drug by maceration / soxhlet method.

 Isolation of chemical constituents from the extract by column

chromatography / separation method.

 Identification of isolated constituents by UV, IR, C13 and NMR spectroscopy.

 Biological activity of the isolated compounds / extract by agar diffusion

method and anthelmintic activity is studied by assay method using earth
worm.

Materials and Methods:

7.
7.1 a) Source of Data:

Search on Medline and other Journals from The Oxford College

of Pharmacy, Govt. College of Pharmacy, RGUHS-Digital library.

b) Place of work: The Oxford College of Pharmacy, Bangalore.

7.2 Method of Collection of Data:

o Collection - Collection of the authenticated plant material (i.e. bark of

Thespesia populnea) from Dr. Harish Botanist. Alva’s education
foundation (R). Alva’s Health centre complex Moodbidri 574227. D.K.
o Extraction - Extracts of the drug will be prepared using different
solvents by maceration / soxhlet.
o Isolation – Isolation of extracted phyto-constituents will be done using
column chromatography \ Separation method.
o Identification and characterization - The isolated compounds will be
identified using analytical methods like UV, IR, C 13 and NMR
spectroscopy.
o Biological activity - The isolated compounds/extract will be studied
for antimicrobial activity by agar diffusion method and anthelmintic
activity is studied by assay method using earth worm.

7.3 - Does the study require any investigations or inventions to be

conducted on patients or other humans or animals?
If so, please describe briefly.

- Not applicable -

7.4 - Has ethical clearance been obtained from your institution in

case of 7.3?
- Not applicable -
8. Bibliography:
1. Khare CP. Indian Medicinal plant 1st ed. Ny:Springer Internatinol Publication;
2007.
2. Puckhaber LS, Stipanovic RD. Thespesenone and dehydrooxoperezinone-6-
methyl ether, new sesquterpene quinones from Thespesia populnea. J Nat Prod.
2004 Sep;67(9):1571-3.
3. Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal Plants. Vol-4 New
Delhi: National Institute of Science Communication; 1995.
4. Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal Plants. Vol-5 New
Delhi: National Institute of Science Communication; 1998.
5. Vasudevan M, Gunnam KK, Parle M. Antinociceptive and anti-inflammatory
effect of Thespesia populnea bark extract. J Ethanopharmacol. 2007 Jan
19;109(2):264-70.
6. Havarasan R, Vasudevan M, Anbazhagan S, Venkataraman S. Antioxident
activity of Thespesia populnea bark extract against carbon tetrachloride-
induced liver injury in rats. J Ethanopharmacol. 2003 Aug;87(2l.-3):227-30.
7. Waller DP, Bunvapraphatsara N, Martin A, Vournazos CJ, Ahmed MS, Soejarto
DD, Cordell GA, Fong HH, Russell LD, Malone JP. Effect of (+)-gossypol on
fertility in male hamsters. Androl. 1983 Jul-Aug;4(4):276-9.
8. Nagappa AN, Cheriyan B. Wound healing activity of the aqueous extract of
Thespesia populnea fruit. Fitoterapia. 2001 Jun;72(5):503-6.
9. Hausen BM, Knight TE, Milbrodt M. Thespesia populnea dermatitis. Am J
Contact dermat. 1997 Dec;8(4):225-8.
10. Shastry CS, Aravind MB, Joshi SD, Ashok K, Bheemachari. Antibacterial and
Antifungal activity of Thespesia populnea. Indian drug. 2005:42(2):81-83.
11. Havarasan R, vasuldevan M, Anbazhagan S, Venkataraman S, Sridhar SK.
Hepatoprotective activity of Thespesia populnea bark extract
against carbon tetrachloride-induced liver toxicity in rats. Natural product
science. 2003 Jun;9(2):83-86.
9. Signature of Candidate

10. Remarks of the Guide

Recommended

11. 11.1 Name and Designation of Guide

S Kambhoja
Professor
Department of Pharmacognosy

11.2 Signature

11.3 Co-Guide
_

11.4 Signature
_

11.5 Head of the Department

Dr. Padmaa M. Paarakh
Professor and Head
Department of Pharmacognosy

11.6 Signature

12. 12.1 Remarks of the Chairman and

Principal Forwarded to the University for scrutiny

12.2 Signature

Dr. Padmaa M. Paarakh

Principal
The Oxford College of Pharmacy,
J.P.Nagar, Ist Phase, Bangalore – 78.