Lab tests for diagnosis Infections by

Enterobacteriacae, Pseudomonas,
Vibrio
Gram negative bacilli
Catalase test
 SELECTIVE Differential MEDIA
MacConkey’s agar DCA, S&S, XLD, EMB, ...

-Supports the growth of most Gram-

negative bacilli For isolation of Shigella
-Differentiate Gram negative bacilli and Salmonella
according to their ability to ferment
lactose into lactose fermenters and non
lactose fermenters
5
Non selective and / differential media

Cystine lactose electrolyte deficient

(CLED) agar

6
Cystine lactose electrolyte deficient
(CLED) agar

7
 ENRICHMENT MEDIA
Selenite F broth Tetrathionate broth Alkaline peptone
water

Enrichment medium Enrichment medium of Enrichment medium

of Salmonella and Salmonella species of Vibrio cholerae
Shigella species from from feces.
faeces
8
LFC
Mucoid LFC
NLFC
 NLF

• Salmonella
• Shigella
Blood culture

13
 NLF

• Proteus
• Pseudomonas (oxidase +ve)
Swarming growth of Proteus
 Exopigment production
production by Pseudomonas
 UTI

• E.coli
• Klebsiella
------------------------------------
• Proteus
• Pseudomonas (oxidase +ve)
Semiquantitative urine culture
 CLED agar
Non selective differential/indicator media for
isolation of urinary pathogens
Quantitative urine culture
E.coli
Proteus
Semiquantitative urine culture
Urea hydrolysis/ Urease test
 Killing- PUNCH
• Klebsiella pneumoniae
• Proteus
• Ureaplasma
• Nocardia
• Cryptococcus neoformans
• Helicobacter pylori
Citrate utilization test
 K C P (Klebsiella Citrate Positive)
• Klebsiella
• Citrobacter
• Pseudomonas aeruginosa
 Citrate Kill PESPS
• Citrobacter
• Klebsiella
• Pseudomonas
--------------------------------
• Enterobacter
• Salmonella paratyphi B
• Proteus mirabilis
• Serratia
 Differ in B.R

Differ in sugar fermentation, amino acid utilization, and

other BRs (I, MR, VP, C, Ur, H2S, gelatinase production …..)

2/17/2019 Microbiology & Immunology Department 33

API (Analytical Profile Index) (E-20)
 Serogrouping agglutination with
specific antisera
-
Gram negative curved bacilli
 SELECTIVE MEDIA
TCBS

Isolation & Differentiation of members of Vibrios into

sucrose fermenters and non sucrose fermenters

39
TCBS
API (Analytical Profile Index)
Serogrouping agglutination with
specific antisera (O1/)139)
43