Professional Documents
Culture Documents
Vitaminc Iodine PDF
Vitaminc Iodine PDF
Solutions Needed
Iodine solution: (0.005 mol L−1). Weigh 2 g of potassium
Method 4. Calculate the concentration in mol L−1 of ascorbic
acid in the solution obtained from fruit/vegetable/
Sample
StarchPreparation
indicator solution: (0.5%). Weigh 0.25 g of 3.juice.Using
Also, the equation
calculate the of the titration (below)
concentration, in mg/100mL
soluble starch and add it to 50 mL of near
For vitamin C tablets: Dissolve a single tablet in 200 mL boiling water determine
or mg/100g of ascorbic acid, in the sampleacid
the number of moles of ascorbic of fruit/
in a 100 mL conical flask. Stir to dissolve
of distilled water (in a volumetric flask if possible). and cool before reacting.
vegetable/juice.
using.
For fresh fruit juice: Strain the juice through ascorbic acid + I2 → 2 I− + dehydroascorbic acid
cheesecloth to remove seeds and pulp which may block Additional Notes
4. Calculate the concentration in mol L−1 of ascorbic
Method
pipettes. 1. acid
Iodine stains
in the both skin
solution and clothing
obtained so proper care is
from fruit/vegetable/juice.
ForSample
packaged fruit juice: This may also need to be
Preparation advised.
Also, If staining
calculate does occur, alcohol
the concentration, may remove
in mg/100mL or mg/
strained through cheesecloth if it contains a lot of pulp skin stains and cleaners are available for
100g of ascorbic acid in the sample of fruit/vegetable/ fabric stains.
For
or seeds. vitamin C tablets: Dissolve a single tablet in 200 mL
of distilled water (in a volumetric flask if possible). 2. juice.
Vitamin C, or ascorbic acid, is a water soluble
For fruits and vegetables: Cut a 100 g sample into small antioxidant that plays a vital role in protecting the
For fresh fruit in
juice: Strainand
the juice through
Add 10cheese
pieces
cloth
and grind
to remove
a mortar
seeds and pulp
pestle.
which may
mL
block
Additional Notesand disease. It is not synthesised
body from infection
portions of distilled water several times while grinding by the human body and therefore must be
thepipettes.
sample, each time decanting off the liquid extract 1.acquired
Iodine stains
from bothsources
dietary skin and– clothing
primarilysofruits
properandcare
intoFora 100 mL volumetric flask. is advised. If staining does occur, alcohol
vegetables. The chemical structure and antioxidant may remove
packaged fruit juice: ThisFinally,
may alsostrain
need the
toground
be
fruit/vegetable pulp through cheesecloth, rinsing the skin stains and
(reducing) cleaners
action are available
of ascorbic acid arefor fabric stains.
illustrated in
strained through cheese cloth if it contains a lot of pulp
pulpor with
seeds. a few 10 mL portions of water and collecting the redox
2. halfC,equation
Vitamin or ascorbic below:
acid, is a water soluble
all filtrate and washings in the volumetric flask. Make antioxidant that plays a vital role in protecting the body
For fruits and vegetables: Cut a 100 g sample into small 3. The concentration of the prepared iodine solution
the extracted solution up to 100 mL with distilled water. from
can be infection and disease.
more accurately It is not synthesised
determined by titrationby with
pieces and grind in a mortar and pestle. Add 10 mL
Alternatively the 100 g sample of fruit or vegetable the
a human
standard body and
solution therefore
of ascorbic must
acid be
or acquired
a standard from
portions of distilled water several times while grinding
may dietary
solution sources – primarily
of potassium fruits andusing
thiosulfate vegetables.
a starchThe
thebesample,
blendedeach in atime
fooddecanting
processoroff together withextract
the liquid
chemical structure and antioxidant (reducing) action of
about
into50 mL mL
a 100 of distilled
volumetric water. After
flask. blending,
Finally, the ground indicator. This should be done if possible as iodine
strainstrain
thefruit/vegetable
pulp through cheesecloth, ascorbic
solutionsacidcanare
beillustrated
unstable. in the redox half equation
pulp throughwashing it with
cheese cloth, a fewthe
rinsing
10mL portions of distilled water, and makeand thecollecting
extracted below:
pulp with a few 10 mL portions of water
solution up to 100washings
mL in a volumetric flask. flask. Make HO HO
all filtrate and in the volumetric O O
oxidation O O
the extracted solution up to 100 mL with distilled water. HO HO + 2H+ + 2e-
reduction
Titration
Alternatively the 100 g sample of fruit or vegetable may HO OH O O