Fah Kasintorn 1001

Liver Enzyme Lab Analysis

The rate of bubbles produced is given a score 1-3 for each condition.
Condition Rating of Bubbles Produced

Normal Liver + Room Temp H2O2 2

Cold Liver + Room Temp H2O2 3

Heated Liver + Room Temp H2O2 2

Normal Liver + Acidified H2O2 3

Normal Liver + Basified H2O2 1

 The time lapse from the time the bubble starts to the time the bubble stops.
Condition Time Lapsed(minutes)

Normal Liver + Room Temp H2O2 20.02

Cold Liver + Room Temp H2O2 20.21

Heated Liver + Room Temp H202 8.53

Acidified Liver + Room Temp H2O2 5.07

Basified Liver + Room Temp H2O2 0.38

Analysis:

For this lab, we were assigned to predict the rate of bubbles that formed in five different
conditions. I have a different hypothesis for each condition. For the first condition my hypothesis
was not supported because the bubbles formed very frequently. My second hypothesis was not
supported because there were a lot of bubbles that were formed. My third hypothesis was
supported because a lot of bubbles were formed. My fourth hypothesis is supported because a
lot of bubbles were formed. My fifth hypothesis is supported because there were very few
bubbles that were formed. According to the results, the cold liver and the normal liver which
were put in the normal room temperature produced bubbles for a very long time and the rate of
bubbles were normal and high. The heated liver which was put in the normal room temperature
hydrogen peroxide produced bubbles for about eight minutes and the rate of bubbles was
normal. For the normal liver in the acidified hydrogen peroxide it only produced bubbles for five
minutes and its rate of bubbles produced is very high. The normal liver that is put in the basified
hydrogen peroxide produced bubbles for only one minute and the rate of bubbles produced was
very low.
I drew the conclusion that the condition normal liver in the normal room temperature
hydrogen peroxide and the normal liver and the acidified hydrogen peroxide were the best
conditions for the enzyme to effectively catalyze their reaction because these two produced a lot
of bubbles. The substrate of this experiment are the liver and the hydrogen peroxide and the
product is the bubbles. The catalase is reusable and it is catabolic. The experiment shows that
the reaction produces bubbles and releases energy. I could improve the experiment by
increasing the time the liver is put into the hydrogen peroxide. There are also some errors such
as the time recorded because we might a bit slow to to stop the stopwatch because we were
also busy with other samples.

Conclusion:

Most of my hypotheses were supported because the rate of bubbles produced were
close to what I predicted before the experiment began. The condition that produced bubbles the
fastest and last the longest was the cold liver in the room temperature hydrogen peroxide and
the condition that produced bubbles the slowest and ended first is the normal liver and basified
hydrogen peroxide.