PULIDO, ALLYSA B.

MICROBPL1 8/31/2019

Experiment No. 2: EXAMINATION OF STAINED PARASITES

INTRODUCTION:
o The microscope is utilized for easy identification and viewing of minute organisms. The
BME compound microscope has two viewing bodies: the monocular teaching bodies and
binocular viewing body. It has built-in illumination system and an internal illuminator and
mirror. For stage preparation, lower the power objectives to have a greater depth of field
and are generally used for initial focusing and viewing. The lower the power objective, the
greater the area of specimen surface will be seen in the field of view.
o When examining a specimen at a high magnification, position the slide so feature is
centred in the field of view. If the feature you want to observe appears to the left of the field
of view move the slide left to the centre and vice versa. By rotating the nosepiece, bring the
next power objective into viewing position. The objective is properly aligned when the
nosepiece click into place.

I. LEARNING OUTCOME
o TO OBSERVE AND DESCRIBE THE STAINED PARASITES SPECIFICALLY AEDES,
ANOPHELES AND CULEX MOSQUITO.

II. EQUIPMENT

MICROSCOPE STAINED LARVAE

IMERSION OIL

COTTON STICK ALCOHOL

 III. PROCEDURE
a) REVIEW INSTRUCTION USED IN MICROSCOPE MANUAL.
b) THE STAINED SLIDE WITH LARVEA WILL BE OBSERVE THROUGH MICROSCOPE
AND IDENTIFY THE SPECIES OF MOSQUITO.

AEDIS EAGYPTI AEDES ALBOPICTUS AEDIS EAGYPTI

AEDIS ALBOPICTUS AEDIS EAGYPTI AEDIS EAGYPTI

AEDIS ALBOPICTUS AEDIS ALBOPICTUS

AEDIS ALBOPICTUS AEDIS AEGYPTI

 c) DRAW MATERIALS USED.
IV. TROUBLESOOTING/ REFLECTION/ SAFETY
Mosquitoes play big role in the transmission of many parasitic and viral infectious diseases. Mosquito-
borne infections are responsible for the death lots of people, thus rendering the mosquito one of the most
dangerous of human enemies. Larvae is the second stage of life cycle of a mosquito and knowing it’s
characteristic and appearance would make us easily identify what specie it belongs, where does it like to linger
and with the knowledge we would know how to get rid of it.
V. QUESTIONS

1) DRAW THE LIFE CYCLE OF A MOSQUITO

2) WHAT ARE THE THREE SPECIES IN TERMS OF APPEARANCE AND CHARACTERISTICS?

 Anopheles- W palps almost as long as the proboscis, the male palps are usually clubbed. When
resting the abdomen is held at an angle from the surface on which the mosquito is standing,
forming a straight line with the proboscis
 Culex- Vary in size but all have a blunt tipped abdomen and usually lack distinctive markings on
the body or wings
 Aedes- Medium to large; most have pointed abdomen and well separated eyes; usually distinctly
marked by patches of dark and light scales; often general appearance of black with silver
markings

3) WHAT ARE THE SPECIFIC DISEASE OF THE THREE SPECIES?

 Culex
 Arbovirus infections transmitted by various species of Culex include West Nile virus, Japanese
encephalitis, St. Louis encephalitis, and Western and Eastern equine encephalitis. Brazilian
scientists are investigating if Culex species transmit zika virus.[5]
 Nematode infections, mainly forms of filariasis may be borne by Culex species, as well as by
other mosquitoes and bloodsucking flies.
 Protist parasites in the phylum Apicomplexa, such as various forms of avian malaria
 Aedes
 Aedes aegypti is a vector for transmitting several tropical fevers.
 The yellow fever mosquito can also contribute to the spread of reticular cell sarcoma among
Syrian hamsters.
 Anopheles
 Some species are poor vectors of malaria, as the parasites do not develop well (or
at all) within them. There is also variation within species. In the laboratory, it is
possible to select strains of A. Gambia that are refractory to infection by malaria
parasites. These refractory strains have an immune response that encapsulates and
kills the parasites after they have invaded the mosquito's stomach wall. Scientists
are studying the genetic mechanism for this response.