Polyploid Abnormalities in

Day 3 and Day 5 Merino

Sheep Embryos
Awin Pinasthika
16/403977/PPT/00944
PENDAHULUAN
 The presence of cytogenetic abnormalities in cleavage-stage
embryos has been correlated
with early embryonic mortality in mammals.
 Analysis must be carried out on cleavage stage embryos to
obtain an accurate assessment
of the frequency of chromosomally abnormal embryos, as
unbalanced embryos are usually
lost before reaching the blastocyst-elongation or egg-cylinder
stage
PENDAHULUAN
 The incidence of chromosome abnormalities in cleavage-
stage sheep embryos has been
estimated to be 60/0 (Long and Williams 1980) and 11%
(Murray et al. 1985a), with the frequency dropping to 1·9%
in day 13-14 blastocysts
PENDAHULUAN
 Long and Williams (1980) principally observed aneuploid
abnormalities (5/6), whereas in the study by Murray et al.
(1985a) 7 of the 8 abnormalities in early embryos were of the
euploid type involving entire chromosome
sets.
TUJUAN
 To determine chromosom aberrant embrio which lost at 3
days until 5 days
 Determine difference in the frequency of chromosom
abnormal embryo
MATERI DAN METODE All day-3
embryos were
collected from
ewes either left
the booster untreated or
injection timed given these
at the morning treatments
such that it was and evening
given 21 days checks for
after the oestrus.
primary two day-5
injection embryos were
collected
from untreated
Two injections of ewes.
Embyo of ewe Fecundin

All day-3
embryos were
collected from
ewes either left
untreated or
at the morning given these
14 days before and evening treatments
expected mating checks for
oestrus.
two day-5
embryos were
collected
from untreated
ewes.
MATERI DAN METODE

Starting 24 h before Embryos were collected Embryos were

sponge removal, ewes at either 48-60 h (day 3; individually
were given four 1-4 cell) or 108-120 h transferred to clean
injections at 12-h (day 5; 16-64 cell) after slides and the excess
intervals of 6, 5, 3 and mating hypotonic
2 by surgically flushing t solution removed.
mg equivalents One drop of
(Armour units), methanol:acetic acid
respectively, of FSH fixative (1:1, v/v) was
(gift from Y. dropped onto the
Combarnous, Embryos were cultured in embryo from
Nouzilly, France) to PBS containing a height of 27 cm.
give 15% sheep serum, 0·8 JLg Chromosome
colchicine ml-1, 100 i.u.
a total dose of 15 mg penicillin G ml-1 and 100 preparations were
Armour equivalents JLg streptomycin sulfate stained in 5% Giemsa
of FSH. ml-1 in 0·004 M phosphate
for 10 h at 37°C with a buffer
5% CO2 in air for 3·5 min, air-dried
atmosphere before and mounted in DPX
preparation of
chromosomes
HASIL

• The frequency of day-3 embryos with euploid abnormalities

is 4 out of 37 or 10,8%

•The incidence of day-5 embryos with euploid abnormalities is thus 15,4 %

(4/26), or, if near-diploids are included 10% (4/40)
HASIL
HASIL
 The frequency of chromosomally aberrant embryos did not
significantly drop between the day-3 and day-5 groups in this
study (10,8% and 15,4%, respectively); this indicates that the
unbalanced chromosomal state of the abnormal embryos is
not lethal until some time after the embryos have entered the
uterine horn
KESIMPULAN
 chromosomally aberrant embryos are not lost before day 5 of
development