Analisis kuantitatif sediaan obat

multikomponen dengan
spektrofluorometri

Preparasi untuk analisis sediaan obat

multikomponen
Tahapan analisis kuantitatif obat
kuantitatif obat
multikomponen
multikomponen dalam sediaan
se diaan obat padat,
semi padat, cair
cair dan steril secara
spektrofluorometri
M. SULAIMAN
SULAIMAN ZUBAIR
ZUBAIR
(www.abuumar80.wordpress.com)
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Istilah
y Analisiskuantitatif : analisis penentuan
penentuan kadar suatu
senyawa
senyawa didalam suatu sampel
y Sediaan obat : padat (tablet, kapsul), semipadat (salep),
cair (sirup, emulsi, suspensi) dan steril (injeksi)
y Multikomponen
Multikomponen : sediaan obat yang terdiri dari beberapa
be berapa
senyawa
senyawa aktif obat, seperti kombinasi GG dan
Difenhidramin dalam sirup obat batuk
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Preparasi sediaan obat multik

multikomponen
omponen untuk
analisis kuantitatif dengan spektrofluor
spektrofluorometri
ometri
- Sampel tablet yang akan dianalisis harus
representatif untuk menghindari resiko adanya
hasil analisis yang keluar dari spesifikasi yang
ditentukan. Contoh : Menurut Farmakope, untuk
analisis tablet parasetamol dibutuhkan sampel
sebanyak
sebanyak 20 tablet parasetamol 500 mg
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Preparasi sediaan obat multik

multikomponen
omponen untuk
analisis kuantitatif dengan spektrofluor
spektrofluorometri
ometri
y Sediaan cair : dapat dilakukan pengukuran
secara langsung, atau diencerkan atau
dipekatkan
dipekatkan terlebih dahulu dengan pelarut
pelar ut
organik
y Sediaan steril (injeksi) : dapat dilakukan
pengukuran secara langsung
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Preparasi sediaan obat multik multikomponen

omponen untuk
analisis kuantitatif dengan spektrofluor
spektrofluorometri
ometri
ySediaan semi padat
Isolasi obat dalam salep harus ditunjukkan
ditunjukkan pada dasar
salepnya :
- Salep lemak bulu domba alkohol, biasanya dilarutkan dalam
kloroform atau eter
- Salep hidrofil, dilarutkan dalam kloroform atau eter

- Salep lanolin, dilarutkan dalam kloroform atau eter

Salep Polietilen glikol, dilarutkan dalam etanol atau air

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y Pada prinsipnya, semua pengukuran

dengan menggunakan
menggunakan instrumen
instr umen
spektroskopi,
spektroskopi, maka
maka syarat pertama
pertama adalah
harus larut dalam larutan pembawa yang
digunakan
y Meskipun sediaan itu merupakan sediaan
obat multikomponen, maka dengan
instrumen spektroskopi
spektroskopi maupun
kromatografi
kromatografi dapat membedakan masing-
masing komponen tersebut, berdasarkan
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Tinjauan Umum Spektrofluorometri

y Spektrofotometri fluoresensi merupakan suatu prosedur
yang menggunakan pengukuran intensitas cahaya
cahaya
fluoresensi yang dipancarkan oleh zat uji dibandingkan
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Instrumentasi
y Pengukuran intensitas fluoresensi dapat
dilakukan dengan suatu fluorometer filter
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Fluoresensi dan Struktur Molekul

y Supaya terjadi fluoresensi, harus terjadi peresapan
cahaya yang kuat oleh suatu molekul. Hal ini
dapat terjadi
ter jadi pada s wa aromati
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Tabel dari beberapa senyawa yang dapat

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Dengan suatu pereaksi tertentu, senyawa yang tidak

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Hubungan Struktur Molekul dan

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kelompok analisis obat secara

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kelompok analisis obat secara

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b. Pengolahan
engolahan Data 4. Laru
Laruta
tan
n stan
standa
darr :
Pengenceran 0,2 ppm
Larut
Larutan
an awal
wal : 100
100 ppm
ppm V1.M1 = V2.M2
1. Dib
Dibuat
uat Larut
Larutan
an St
Stok
ok : 1 V1.1
V1 .1 ppm
ppm = 25 mL.
mL.
ppm 0,2ppm
V1 = 5 mL
Perhitungan 5. Laru
Laruta
tan
n St
Stan
and
dar :
V1.M1 = V2.M2 0,25 ppm
V1.1
V1 .100
00 ppm
ppm = 100
100 mL.
mL. 1ppm
1ppm V1.M1 = V2.M2
V1 = 1 mL V1.1
V1 .1 ppm
ppm = 25 mL.
mL.
2. Larutan
Larutan stand
standar
ar : 0,1 ppm
ppm 0,25ppm
V1 = 6,25 mL
V1.M1 = V2.M2 6. Laru
Laruta
tan
n St
Stan
and
dar :
V1.1
V1 .1 ppm
ppm = 25 mL 0,1p0,1ppm
pm 0,3 ppm
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y Tabel 2. Pengolahan Data

Bahan nsitass pada  =450
Intensita
Inte Intensita
Intensitass Bahan-
Bahan-Blank
Blanko
o
nm pada  =450nm

Blanko 0,4 -
Sampel 31 30,6
0,1 ppm 14 13,6

0,15 ppm 19.9 19,5

0,2 ppm 26 25,6

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y Dari grafik didapatkan persamaan regresi:

y = bx + a
I = m.C + Io
I = 124,2 C + 0,94
Dengan perhitungan kalkulator didapat R2 = 1
Maka dapat diketahui konsentrasi sampel dengan cara substitusi nilai
intensitas sampel dikurangi blanko pada panjang gelombang
ge lombang 450nm.
I sampel = 30,6
I = 124,2 C + 0,94
I = 124,2 C + 0,94
C = 0,23 ppm
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Sekian dan
Terimakasih

WASSALAM