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Introduction
Osmosis is a specialized case of diffusion that involves the passive transport of water. In osmosis,
water moves through a selectively permeable membrane from a region of its higher concentration to a
region of its lower concentration. The membrane selectively allows passage of certain types of molecules
while restricting the movement of others.
The solute concentration in the beaker is higher than that placed in the dialysis bag, and thus the
water concentration is lower in the beaker than in the bag. This causes water to move from the bag into the
beaker. This can be observed by adding a dye to the solution within the dialysis bag.
Procedure
The first step of this experiment is to fill an empty beaker with a 0.8M solution given by the
instructor, followed by preparing a dialysis semi-permeable bag in which to measure osmosis. To prepare
the bag, one must first gently loosen the two semi-permeable walls from one another to open what now
looks like a flimsy tube. Gently, tie off one end of the tube tightly with the yarn. Next, fill the bag with a
0.2M solution given by the instructor and add one drop of green food coloring. Finally, tie off the open end
of the bag as tightly as possible.
Once the beaker and bag has been prepared, the next step is to weigh and record the initial weight
of the semi-permeable bag. After completing this step, one is to place the prepared bag into the prepared
beaker and wait for about eight minutes.
After allowing the bag to sit inside the solution within the beaker, the bag is removed from the
beaker and weighed again. Finally, this new weight is subtracted from the initial weight to show how much
water the dialysis bag lost due to osmosis. Finally, to further prove osmosis occurred, one is to look at the
remaining solution in the beaker. If there is a hint of green, then one can conclude that osmosis took place.
Questions
An isotonic solution occurs when there is an equal concentration of particles on each side of a
semipermeable membrane. In order for particles to move, there needs to be a concentration gradient. A
gradient occurs when there are unequal concentrations of particles on either side of a semipermeable
membrane. Because there is no difference in concentration between two isotonic solutions, there will be no
net movement of water between them.
In order for cell to expand due to osmotic forces, it needs to be placed in a hypotonic solution. A
hypotonic solution has a relatively low concentration of dissolved particles, when compared to the amount
of dissolved particles on the inside of a cell. The liquid portion of the blood is considered to be isotonic to
red blood cells. For this reason, there is no net movement of water inside or out of the cell, causing the cell
to remain a constant size.
Name: ________________
Period:________________