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Bacillus Anthracis Powerpoint
Bacillus Anthracis Powerpoint
Domain : Bacteria
Phylum : Firmicutes
Class : Bacilli
Order : Bacillales
Family : Bacillaceae
Genus : Bacillus
Species : Bacillus anthracis
Bacillus cereus
orical Interests
ical Interests
First pathogenic bacteria to be seen under the Microscope - 1849 Pollender
rst pathogenic bacteria to be seen under the Microscope - 1849 Pollender
First communicable Disease - Transferred Experimentally - Inoculation of infec
rst communicable Disease - Transferred Experimentally - Inoculation of infecte
- blood ( Davaine 1850 )
- blood ( Davaine 1850 )
First bacteria to be isolated in Pure Culture & shown to posses spores – B. anthra
rst bacteria to be isolated in Pure Culture & shown to posses spores – B. anthraci
- Robert Koch 1876
- Robert Koch 1876
First bacteria employed for preparation of attenuated vaccine - Louis Pastuer
rst bacteria employed for preparation of attenuated vaccine - Louis Pastuer
ENERAL
ENERALCHARACTERISTICS
CHARACTERISTICS
- Large , gram(+) bacilli, encapsulated, non-motile,
spore former -centrally located
Serpentine chains (+) Catalase positive
- Metabolically diverse - Strict aerobes & facultative anaerobes,
Psychrophilic, mesophilic, thermophiles,
Alkalophilic, Neutrophilic, and acidophilic
species.
- Formation of spores
In culture - aerobically & anaerobically
in nature ( Soil ) - shed by animals.
- Fresh specimen- stained gram (+)
With age - stained Gram(-) or variable.
CLINICAL SYNDROMES:
1. Cutaneous Anthrax – 1-7 days
- Small papule at site of injection, ring of vesicles develops,
coalesce,
Erythematous ring.
- Small dark area at center of the ring ulcerates & dries
Depressed
- Black necrotic central area Black Eschar or Malignant
Pustule.
( 7-10 days )
- Lesions painless , no pus unless secondarily infected.
- Healing :1-2 weeks - Granulation dislodge and leaving a
scar.
- Complications : Sepsis - Meningitis
2. Inhalation Or Woolsorter Disease
2. Inhalation Or Woolsorter Disease
- Incubation period – up to 6 weeks
- Incubation period – up to 6 weeks
- Mediastinal lymph nodes
- Mediastinal lymph nodes
- Marked hemorrhagic necrosis edema – Mediastinum
- Marked hemorrhagic necrosis edema – Mediastinum
- CXR – Pronounced Mediastinal widening
- CXR – Pronounced Mediastinal widening
- Mimic viral respiratory infection
- Mimic viral respiratory infection
Rapidly progressive Severe pulmonary disease
Rapidly progressive Severe pulmonary disease
- Complications : Hemorrhagic pleural effusion
- Complications : Hemorrhagic pleural effusion
Sepsis
Sepsis
Bowel ulcerations
Bowel ulcerations
Hemorrhagic meningitis
Hemorrhagic meningitis
- Associated with higher mortality.
- Associated with higher mortality.
- 2001 Anthrax bioterrorism – 22 cases
- 2001 Anthrax bioterrorism – 22 cases
3. Gastrointestinal Anthrax
- Ingestion of poorly cooked infected meat
Manifestations: Abdominal pain, bloody diarrhea, fever and
vomiting
- Associated with higher mortality.
PREVENTION AND TREATMMENT;
1. Vaccination of animal herds
2. Vaccination of humans – Ava BioThrax - contains PA – Toxigenic
strains
- Supernatant cell-
free culture
- 0,4 weeks , 6,12,18
months
- U.S Department of
Defense
TREATMENT:
1. Penicillin G
2. Alternative Drugs : Tetracycline and chloramphenicol
3. Streptomycin or gentamicin
4. Ciprofloxacin or Doxycycline – setting of Potential exposure
( Bioterrorism )
- Up to 4 weeks
Note: Antibiotic does not change progression of disease only
prevents dissemination
cillus cereus
milar in morphology with B anthracis but are motile & is not susceptible to Peni
auses gastroenteritis associated with = FRIED RICE
her diseases: Traumatic eye Infection - Panopthalmitis
Catheter-associated sepsis
Rarely- Severe pneumonia
Mediated by 1 of 2 Toxins:
1. Heat-labile Enterotoxin = Diarrhea forms
2. Heat-Stable Enterotoxin = Emetic form
hree Toxins:
1. Necrotic toxin – Heat labile enterotoxin
2. Cereolysin – Hemolysis
3. Phospholipase C – Lecithinase
cillus subtilis
A common laboratory contaminant
Associated with infections in immunocompromised patients.
COMPARISON OF EXOTOXIN PRODUCED BY BACILLUS
COMPARISON OF EXOTOXIN PRODUCED BY BACILLUS
CEREUS
CEREUS
CHARACTERISTICS DIARRHEAL TOXIN
CHARACTERISTICS DIARRHEAL TOXIN
EMETIC TOXIN
EMETIC TOXIN
BACILLUS CEREUS GROWTH CULTURE
REFERRENCES
1. Jawetz Medical microbiology
2. Medical microbiology – Ellen Baron
3. Medical Microbiology – Zinsser
4. MIMs Medical Microbiology
5. Microbiology – Bauman
6. Microbiology – Nester
7. Medical Microbiology - Chakraborty