Sop of cleaning of glassware and garments.

1.0 Objective

To lay down a procedure for cleaning and sterlization of glassware and garments.

2.0 Responsibility

Microbiologist/QC-Executive.

3.0 Accountability

Head-QC executive

4.0 Procedure

4.1 Cleaning of Glassware

4.1.1 The cleaning of glassware should be done as follows:

4.1.2 All the media in the used Petri plates and test tubes should be removed and collected
in disposal bag.

4.1.3 The glassware should be transferred in washing area.

4.1.4 The glassware washed with potable water, followed by washing with teepol solution
and again with soft water.

4.1.5 Finally the glassware washed with purified water to remove the traces of Teepol
solution in / on the glassware.

4.1.6 The glassware should be dried in the Hot air oven.

4.2 Sterilization of glassware

4.2.1 Sterilized Glassware in autoclave.

4.2.2 All the Petri plates shall be individually wrapped in a single layer of butter paper or

directly place in S.S. Container.

4.2.3 Place these wrapped Petri plates in a S.S container and sterilized by autoclaving at
121°C Screw cap sampling bottles shall be loosely capped and sterilized by
autoclaving at 121°C for 15 minutes in autoclave.

4.2.4 After the completion of the sterilization cycle, autoclave is opened; these bottles shall
be screwed tightly.
 4.2.5 Test tubes are plugged with cotton when sterilized in autoclave.

4.3 Sterilization of Garments .

4.3.1 Used garments shall be sent to laundry for washing.

4.3.2 After receiving washed garments from laundry, the same shall be put in Hi-media bag
for sterilization.

4.3.3 Place these bags (wrapped garments) in autoclave for sterilization at 121°C for 25
minutes.
4.3.4 Subsequently the dried clothes bag shall be kept in cabinet of the change room for use.