Professional Documents
Culture Documents
Iodometric Analysis For Vitamin C Lab Report PDF
Iodometric Analysis For Vitamin C Lab Report PDF
8
“Iodimetric Analysis for Vitamin C”
OBJECTIVES:
1. To prepare and standardize an iodine solution;
2. To analyze an unknown sample containing vitamin C through titration
method.
DISCUSSION:
RESULTS:
TRIAL 1 TRIAL 2
CALCULATIONS:
5.2 mL I2.
+ 5.4 mL I2
10.6 mL I2
10.6 mL I2 ÷ 2 = 5.3 mL I2
Trial 1:
5.3 mL I 2 0.10 g AA
=
0.5 mL Vitamin C X
X =9.43 ×10−3 g AA
1 mol
9.43 ×10−3 g AA ( 176 g)
=5.36 ×10 −5
mol AA∨mol Vitamin C
Trial 2:
5.3 mL I 2 0.10 g AA
=
0.4 mL Vitamin C X
X =7.55× 10−3 g AA
Trial 3:
5.3 mL I 2 0.10 g AA
=
0.4 mL Vitamin C X
X =7.55× 10−3 g AA
7.4646 mg
24. 2556 mg
X ( 8.0852 )=( 10 mL )( 60 mg )
CONCLUSION:
Through proper titration techniques together with the results gathered and
calculations conducted, the vitamin C content of orange fruit is 74.21 mL which is
needed to attain 60 mg Recommended Dietary Allowance (RDA).
ANSWERS TO QUESTIONS:
1. Write a balanced equation for the reaction between I2 and ascorbic acid.
Identify the oxidizing agent and the reducing agent.
OA: C6H6O6
RA: C6H8O6
1 mol C 6 H 8O 6
0.1 g C 6 H 8 O 6 ( 176.9 C 6 H 8O 6 )
−4
=5.65 x 10 mol
1 mg AA
176.1 mg AA = 0.005679 mmole AA
0.005679mmole AA
mmol = 0.25 mL
0.02243
mL
1 mg ascorbic acid
Titer = 0.25 ml I 2 sol ' n
3. A sample of fresh grapefruit juice was filtered and titrated with the above I 2
solution. A 100-mL sample of the juice took 9.85 mL of the iodine solution to
reach the starch end point.
25.32 ml 9.85 ml
=
0.10 g x
60 mg
=156.02mL of this fruit juice is needed
38.457