FUS-100 Service Manual PDF

Service Manual of FUS-100 Urine Sediment Analyzer
FUS-100 Urine Sediment Analyzer
Service Manual
Preface
Introduction:
♦ Please read the User Manual and Service Manual carefully after unpacking.
♦ Installation, debugging and maintenance should be executed in strict accordance with the
Manual.
♦ Keep the Manual safely for your any time reference.
♦ Incorrect use may affect instrument accuracy and precision or endanger personal safety.
Note:
♦ Instrument should be operated by medical inspection specialist, physician, nurse or lab
assistant whom are specially trained.
♦ Instrument should be controlled by special software. Please install the software that is
appointed by our company. Installation of other software/hardware may interfere normal
working. Don’t run other software when instrument is working.
♦ Dust may accumulate on the surface of instrument after long time storage. Clean soft cloth or
gauze can be used for cleaning work, and a little detergent can be used if necessary. Please
cut off the power supply before cleaning.
♦ As to the use and storage method of Control, Standard Solution and Focus, please refer to the
relevant instruction.
♦ Sample, Controls, Standard Solution, Focus and waste solution, etc. have potential
biochemical infectivity, the detergents are corrosive that may hurt eyes, skin and mucosa.
Operator should refer to the safety regulation for lab operation. Protective measure should be
taken (Such as lab protective clothes and gloves).
♦ Avoid contact with eyes and skin, in case of skin contact, rinse immediately with plenty of
water and seek medical advice.
♦ Operator should comply with the local regulation when draining and dealing with reagent,
waste solution, waste sample, consumable, etc. Please dispose the waste solution and
instrument consumable according to the regulation of medical waste, infective waste and
industrial waste.
Warning:
♦ Instrument should be operated in a good ground condition, and an independent power supply
is a must, the input power should conform to instrument requirement.
♦ Don’t pull the electrical wire with wet hand, or there is a risk of electrical shock.
♦ Don't stamp, twist or drag the wire and cable, or it may cause a fire.
♦ Please don’t open the back and side cover board before cutting the general power supply
except DIRUI service staff.
♦ If liquid occurs inside instrument or there is an internal pipeline leakage, please cut off the
general power supply immediately, and contact DIRUI service dept.
♦ Periodic maintenance should be executed strictly according to the User Manual and Service
Manual. Or it may cause instrument malfunction, or affect the accuracy and precision of test
results.
♦ Make sure that the Urine Sediment Analyzer is operated according to the User Manual, or the
test result will not be reliable, and the damage on instrument may endanger personal safety.
♦ Please don’t place combustible material around the instrument.
Content
Chapter Ⅰ Introduction .................................................................................................................. 6
1.1 Urine Sediment Analyzer Overview ................................................................................... 6
1.2 Test Principle ....................................................................................................................... 6
1.3 System Principle Diagram................................................................................................... 6
1.3.1 Instrument Compose .............................................................................................. 6
1.3.1.1 Front View ..................................................................................................... 6
1.3.1.2 Rear View ...................................................................................................... 8
1.3.1.3 Right Side View ............................................................................................ 8
1.3.2 Air and Liquid Principle Diagram ............................................................................ 9
1.3.3 Electric Principle Diagram ....................................................................................... 9
1.3.4 Optics Principles Diagram ..................................................................................... 11
1.4 Test Item and Image .......................................................................................................... 12
1.5 Marks ................................................................................................................................ 13
Chapter Ⅱ Installation .................................................................................................................. 14
2.1 Installation Instruction ...................................................................................................... 14
2.2 External Device Connection ............................................................................................. 14
2.2.1 Offline Mode .......................................................................................................... 14
2.2.2 Online Mode .......................................................................................................... 14
2.2.3 Software Installation .............................................................................................. 14
Chapter Ⅲ Focus and Calibration ................................................................................................. 15
3.1 Focus ................................................................................................................................. 15
3.2 Calibration......................................................................................................................... 15
Chapter Ⅳ QC .............................................................................................................................. 17
4.1 Control .............................................................................................................................. 17
4.2 QC Register and QC Setting ............................................................................................. 17
4.3 QC Test ............................................................................................................................. 19
4.4 QC Review ........................................................................................................................ 20
4.4.1 QC Result Inquiry .................................................................................................. 21
4.4.2 QC Result Modification ......................................................................................... 25
4.4.3 Add QC Result ....................................................................................................... 26
4.4.4 Delete QC Result.................................................................................................... 26
4.5 QC Stat. ............................................................................................................................. 26
4.5.1 QC Chart ................................................................................................................ 27
4.5.2 About QC ............................................................................................................... 28
Chapter Ⅴ Sample Test ................................................................................................................ 30
5.1 Test Preparation ................................................................................................................. 30
5.1.1 Note for Sample Preparation .................................................................................. 30
5.1.2 Sample Volume ...................................................................................................... 30
5.1.3 Tube Requirement .................................................................................................. 30
5.1.4 Barcode Using Requirement .................................................................................. 30
5.1.5 Check Waste Liquid, Sheath and Printer ................................................................ 32
5.2 Off-line Test ...................................................................................................................... 32

5.2.1 Plug in Power Supply & Register Software ........................................................... 32
5.2.2 Instrument Status Confirm ..................................................................................... 32
5.2.3 Offline Sample Test ................................................................................................ 33
5.3 On-line Test ....................................................................................................................... 34
5.3.1 Plug in Power Supply & Register Software ........................................................... 34
5.3.2 Instrument Status Confirm ..................................................................................... 34
5.3.3 Online Sample Test ................................................................................................ 34
5.4 Testing Process .................................................................................................................. 37
5.4.1 Patient Information Editing .................................................................................... 37
5.4.2 Alarm Information Confirm ................................................................................... 38
5.4.3 Delete Alarm Information ...................................................................................... 38
5.5 Sample Review ................................................................................................................. 39
5.6 Test Result ......................................................................................................................... 39
5.7 Sample Examination ......................................................................................................... 42
5.8 Report Preview and Print .................................................................................................. 43
5.9 Result Delete ..................................................................................................................... 44
Chapter Ⅵ Database Backup ........................................................................................................ 45
Chapter Ⅶ Troubleshooting .......................................................................................................... 46
7.1 Main Unit Maintenance..................................................................................................... 46
7.1.1 Calibration Failed ................................................................................................... 46
7.1.2 Too Much Air Bubble ............................................................................................. 46
7.1.3 M5、M4、M3 Motor Error ................................................................................... 46
7.1.4 Electric Level Error ................................................................................................ 47
7.2 Mechanical System Maintenance ...................................................................................... 47
7.2.1 Counter in Sample Carrier ..................................................................................... 47
7.3 Optical System Maintenance............................................................................................. 48
7.3.1 Focus Motor ........................................................................................................... 48
7.3.2 Focus Failure .......................................................................................................... 48
7.3.3 Optical Fiber Sensor Error ..................................................................................... 48
7.4 Liquid Tubing System Maintenance ................................................................................. 48
7.4.1 Sheath Insufficient Alarm....................................................................................... 49
7.4.2 Waste Tubing Block ............................................................................................... 49
7.4.3 Syringe Pump Make Sound .................................................................................... 49
7.4.4 Small Value ............................................................................................................ 50
7.4.5 Air Pump Keep on Working ................................................................................... 50
7.5 Software System Maintenance .......................................................................................... 50
7.5.1 Installation（FUS100 connect with H800） ......................................................... 50
Chapter Ⅰ Introduction
1.1 Urine Sediment Analyzer Overview

Developed and produced by DIRUI INDUSTRIAL CO., LTD., FUS-100 Urine Sediment
Analyzer is used for in vitro diagnosis. By connecting with DIRUI H-800 Automatic Urine
Analyzer, urinalysis workstation is formed. Routine urinalysis (Such as cells, bilirubin, WBCs,
glucose levels and urine specific gravity, turbidity) and urine visible component counting (Such as
cells, cast, crystal, etc.) can be conducted by the workstation.
In addition, FUS-100 Urine Sediment Analyzer can be used as an independent device, its result
can be combined with other urine analyzer test result.
1.2 Test Principle

FUS-100 Urine Sediment Analyzer adopts the flow microscopy imaging system. The sheath
packed sample flow into the system under the power of mechanical system which is composed of
specially made thin layer plate. The injector pump pushes the sheath into the flow cell which is
composed of thin layer plate. Under the effect of double layer sheath, urine sample enter into the
flow cell in the depth of single cell. Then images will be captured at high speed. The urine sample
will be discharged into wasted container after test, as figure shows:
1.3 System Principle Diagram

1.3.1 Instrument Compose
1.3.1.1 Front View
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①“Start” Button ②Key Switch ③Sample Carrier
Figure 1-1
“Start” button is used for upgrade program, its usage method is as follow:
Switch off the power switch under standby, press “Start” button, switch on power switch, release
“Start” button. Open the upgrade software within the computer, select the document need to be
upgraded. Start upgrading, switch off power and restart after upgrading.
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1.3.1.2 Rear View
①RS-232 ② Sheath Inlet ③Waste Outlet ④Power
⑤Power Switch ⑥Camera Port ⑦Fan
Figure 1-2
1.3.1.3 Right Side View
Figure 1-3
Note：the fixing baffle should be removed when connecting with Urine Analyzer.
8
1.3.2 Air and Liquid Principle Diagram
1.3.3 Electric Principle Diagram
Figure 1-4 General Circuit
9
Figure 1-5 Main Control Board Circuit（1）
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1.3.4 Optics Principles Diagram
1. Pulse Xenon Lamp 2. Illuminator 3. Aperture Stop
4. Green Filter 5. Condenser 6. Object Plane
7. Objective Lens 8. Attachment Objective 9. CCD Camera
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1.4 Test Item and Image

Type Ab. Image
Red Blood Cell RBC
White Blood Cell WBC
White Blood Cell

WBCC
Clumps
Squamous Epithelial
SQEP
Cell
Non-Squamous
NSE
Epithelial Cell
Bacteria BACT
Crystal CRYS
Hyaline Cast HYAL
Pathological Cast UNCC
Yeast BYST
Sperm SPRM
Mucus MUCS
Note：The disk equipped with the instrument contains classic cell images.
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1.5 Marks
Marks Meaning
The prompts to pay attention, otherwise, may result in personal injury.
Biology pollution mark

Warning operator there is potential biology pollution danger.
Laser Warning
Avoid injury, do not look directly at the laser beam
AC mark
Only for in vitro diagnosis use
Storage temperature
Lot No.
Warranty date
Series No.
Calculate allowed mark
Date of manufacture
Manufacturer
Grounding mark
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Chapter Ⅱ Installation
2.1 Installation Instruction
The instrument installation shall be executed by professional. And the space, power supply and
environment requirement should be met. If the installation is conducted by the user, User Manual
should be read carefully beforehand.
2.2 External Device Connection
2.2.1 Offline Mode

Remove the sample carrier cover before its installation (hold the sample carrier cover with both
hands, and lift upward).
Take down the tie wire used for fixing sample probe after sample carrier installation, otherwise,
the instrument can not work.
The connection should be conducted in strict accordance with the User Manual.
2.2.2 Online Mode

Refer to User Manual.
2.2.3 Software Installation

Install SQL database before FUS-100 Urine Sediment Analyzer application software installation.
Legal copy 2005 or above version is recommended. Our company will not provide database.
The online software will be installed by professional, the user shall not uninstall the software
except particular condition. If the software needs to be reinstalled or uninstalled, follow the user
manual.
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Chapter Ⅲ Focus and Calibration
3.1 Focus
In order to make sure the visible component in urine is captured clearly when passing through
focusing plane of microscope, daily focus should be conducted before test.
Note：
1. Plastic test tube or plastic pipette can not be used in focus.
2. In order to capture the clear images, the Focus specified by DIRUI should be used.
3. Daily focus should be conducted after booting.
3.2 Calibration
(a) Standard Solution Storage
♦ Standard Solution is the solution containing RBC, refer to instruction for cell volume.
♦ Standard Solution should be stored at 2--8℃. It can be used after placing at room temperature
for a while.
(b) Standard Solution Usage
♦ Shake before use：inverse the Standard Solution bottle, shake back and forth for 5 times, and
shake the bottle for 5 times, it can be used when no air bubble exists.
♦ The standard solution should be used immediately after unseal. Each bottle is for single time
use, the left can not be used. Standard solution mixture with different lot No. can not be used.
(c) Calibration Frequency
♦ Monthly FUS-100 Urine Sediment Analyzer calibration should be conducted, focus should be
conducted before calibration.
(d) Calibration Preparation
♦ Take 10 glass test tubes, inject 4ml Standard Solution in each tube.
♦ Place 10 test tubes in the test tube rack.
(e) Calibration Note
♦ Plastic test tube or plastic pipette can not be used in calibration.
♦ Please use the Standard Solution specified by DIRUI.

(f) Calibration Test
♦ Place the test tube rack on the right of the sample carrier, click “Calib” in figure 3-1, refer to
15
3-2 for its display:
Figure 3-2
♦ Input lot No. and Mean Value of Standard Solution in figure 3-2, click “Calib”, sampling
probe will conduct sampling automatically. The calibration status will be displayed in the
interface after 10 test tube test has been finished.（meanwhile, “System Monitor” interface
will display calibration time and calibration coefficient.）. The “Status” column will display
“Pass” if calibration succeed. The instrument will enter into standby automatically.
♦ The “Status” column will display “Fail” if calibration failed. Conduct calibration again after
standard solution replacement (with another lot No.) . Contact the after-sale service dept. or
distributor of DIRUI if calibration still failed.
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Chapter Ⅳ QC
QC function is to check and test analysis accuracy through testing urine control and collecting QC
data.
QC purpose is to make sure test result accuracy and repeatability.
Note：Operate in strict accordance with the steps.
4.1 Control
(a) About Control
Control is used for QC of dimming and test result. Positive Control is used for abnormal QC,
negative Control is used for normal QC, both positive Control and negative Control are used for
checking whether the instrument can conduct counting accurately.
Positive Control is the ionic equilibrium solution containing fixed volume RBCs. Refer to the
User Manual for cell volume.
Negative Control does not contain particle.
Positive Control and Negative Control must be refrigerated and placed at room temperature for a
while before use.
(b)QC Frequency
Positive and negative QC should be conducted at least once a day, or according to the standard of
lab QC procedure manual. Focus should be conducted before QC.
4.2 QC Register and QC Setting
Click , , as figure 4-1 shows:
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Figure 4-1
(a)Add QC Item
Input corresponding parameter in the input box behind “Model”, “Mean Value” and “Name”.Click
the drop-down menu behind “Validity”, select the validity marked on the QC instruction, and click
“Add”.
(b)Delete QC Item
Select the QC item need to be deleted in QC register list(as figure 4-1 shows), click “Delete”, as
figure 4-3 shows:
Figure 4-3
Click “Yes”, the selected QC item will be deleted.
(c)QC Setting
Click “QC Setting” in figure 4-1, as figure 4-3 shows:
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Figure 4-3
1SD、2SD、3SD means 1 time, 2 times and 3 times standard deviation for test result. If the
【Exceed 3SD】alarm range is selected, the instrument will prompt alarm when the QC result
exceeds 3SD.
Select the commonly used alarm range, click , as Figure 4- 4 shows:
Figure 4-4
Click “Yes” to save the new QC range.
4.3 QC Test
(a)Control Usage
Shake before use: inverse the Positive Control bottle, shake back and forth for 5 times, and shake
the bottle for 5 times, it can be used when no air bubble exists.
Note: Negative Control does not contain particle, shaking is not needed.
(b)QC Test Note
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♦ Plastic test tube or plastic pipette can not be used in QC.
♦ Please use the Control specified by DIRUI.

(c)QC Test Preparation
♦ Inject 3ml Positive Control, 3ml Negative Control into two test tubes.
♦ Place the 2 test tubes in the test tube rack according to the sequence of QC register.
(d)QC Test
♦ Click “QC” in figure 4-3, as figure 4-5 shows:
Figure 4-5
♦ Place the test tube rack on the right of the sample carrier. Select the box in front of the lot
No.( ) need to be tested in QC register interface. Click “QC”, the sample

probe will conduct sampling automatically. QC related information and QC status(pass or fail)
will be displayed after test. Test tube rack will be moved to the left of the auto-sampler,
instrument enter into standby.
♦ The “Status” column will display “Fail” if QC failed. Conduct QC again after Control
replacement (with another lot No.). Contact the after-sale service dept. or distributor of
DIRUI if QC still failed.
4.4 QC Review
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Click “QC Review”in figure 4-5, as figure 4-6 shows, the tested QC information can be inquired.
Figure 4-6
4.4.1 QC Result Inquiry
Click “Inquire” in figure 4-6, as figure 4-7 shows:
Figure 4-7
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(a) Inquire through lot number
Select the lot number (for example select lot number 000001) in the box under lot number and
click button, shown as figure 4-8:
Figure 4-8
All QC result with lot number 000001 will be displayed on the screen, click to get
the preview for QC result , shown as figure 4-9.
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Figure 4-9
Click button for the print of the QC result.
(b) Inquire through QC name
Select the QC name in the list of name(for example select negative) in the box under name in
figure 4-7 and click button, shown as 4-10:
23
Figure 4-10
All negative QC result will be displayed on the screen, click button to get the
preview for QC result as shown in figure 4-9. Click button for the print of the QC result.
（c）Inquire through status
Select the status after QC test (for example select pass) in the box under status in figure 4-7 and
click button, shown as 4-11:
Figure 4-11
All passed QC result will be displayed on the screen, click button to get the preview
for QC result as shown in figure 4-9. Click button for the print of the QC result.
（d）Inquire through date-time
Select the box in front of【use date –time】in figure 4-7 , choose the start & end date, time and
click button, shown as 4-12:
24
Figure 4-12
All QC result within the time will be displayed on the screen, click button to get the
preview for QC result as shown in figure 4-9. Click button for the print of the QC result.
4.4.2 QC Result Modification

Select the line of the QC result need to be modified in figure 4-6 (the screen will display dialogue
if not selected) and click button to get figure 4-13:
Figure 4-13
25
Select the lot number, name and date and time of QC test need to be modified and input QC
counting value, click button to modify.
Note: the low value, high value and mean value of QC test can not be modified.
4.4.3 Add QC Result
Click in figure 4-6, as figure 4-14 shows:
Figure 4-14
Select the QC lot No., name and date, time of QC test need to be added. Input the low value, high
value and counting value of QC test, click “OK”.
4.4.4 Delete QC Result

Select the line of QC result need to be deleted in QC list in figure 4-6, click “Delete”. And click
“Yes” to delete the QC result.
4.5 QC Stat.
Click in figure 4-6, as figure 4-16 shows to review and print QC chart.
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Figure 4-16
4.5.1 QC Chart
(a)About QC Chart
The abscissa of the QC chart is the date of the QC, ordinate is the QC line.
QC chart can display 31 points at most.
Each point correspond to 1 QC result, the points are connected by segment.
(b)QC Chart Review and Print
Click the drop-down list behind QC date, select the QC date; select the QC lot No. in the
drop-down list behind QC lot No., and select the QC name in the drop-down list of QC name, the
inquired result will be displayed in the QC chart.
Click “Print” to preview the QC chart, as figure 4-17 shows:
27
Figure 4-17
Click to print the QC chart.

4.5.2 About QC
x (mean value)：the mean value for a group of number, and calculate with the following formula:
∑x
i =1
i
x=
n
SD(standard deviation)：a statistics method to describe the dispersion degree for a group of test
value, and calculate with the following formula:
∑ (x
i =1
i − xi ) 2
SD=
n −1
N for test times
xi the test value for each time.
CV valve (coefficient of variation): the ratio between standard deviation and mean value called
28
CV value, it is a statistics reflect the variation degree for each test result, and calculate with the
following formula:
SD
CV = × 100%
x
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Chapter Ⅴ Sample Test

Warning:
1. If the foam on the sample is higher than 5 mm, please wait until the foam is lower than 5mm.
2. The operator must keep away from the position for sampling probe to avoid being hurt.
3. Avoid testing thickness samples which may jam the probe.
4. If there is blood urine sample by eye, put it on the last position of the tube rack. Press “STOP”
button after sampling , and the instrument will do automatic rinsing and re-start test.
5. The waste container should be put on the floor and the waste liquid pipe keeps vertical to make
sure the waste liquid running smoothly.
5.1 Test Preparation
5.1.1 Note for Sample Preparation

(a) Collect urine with clean or sterilized vessel.
(b) Use fresh urine sample. If it is not tested within one hour after collection, seal the sample and
store it at 2-8℃. Get the sample back to room temperature before test.
(c) Do not add antiseptic, disinfectant or detergent into the sample.
(d) Keep the urine sample away from sunshine.
(e) Fully mix the urine sample before test but do not use centrifuge or it may influence the test
result.
(f) If the urine sample contains ascorbic acid, the test result for Glucose may be lower than true
value (Dry Chemistry Result).
(g) The sample must be tested under room temperature. If the sample temperature out of this range,
the result for SG may be incorrect. (Dry Chemistry Result)
5.1.2 Sample Volume
The minimum urine volume is 4.0 ml（Use FUS -100 alone and connecting with DIRUI H-800）.
Note：
Do NOT fill the test tube at any time, because auto sample mixing will be conducted before
sampling, sample overflow should be avoided.
5.1.3 Tube Requirement
（a）Sample tube standard: 16mm*100mm tube, the tube mouth must be regular without
deformation.
（b）Sample tube rack standard: use the supplied sample tube rack.
5.1.4 Barcode Using Requirement
（a）Bar code type: CODE128，CODE39，CODE39 and CODEBAR.
（b）Print width for barcode should between 8～12mm. And the valid length for barcode should
30
be less than 40mm.The start blank and finish blank on the barcode should be more than 3mm.
And the number on barcode should be more than 4 bits and smaller than 18 bits. As shown in
figure 5-1:
Figure 5-1
(c) Stick requirement for barcode：

1. Barcode stick must flat without any pollution and the print for barcode lines should be complete
or the instrument can not read the barcode.
2. Barcode should keep 15mm-20mm from the tube bottom as shown in figure 5-2. And confirm
you can see all the barcode stick from the tank hole when put these tubes in tube rack. And make
sure the tube close to the bottom of the tube rack:
Figure 5-2
3. When the barcode is CODE39 small letter, the scanned ID number on screen and print report
（）on the barcode or the barcode

will be corresponding big letter plus“+”, there should be no ‘’、“”、
can not be normally recognized.
4. For example：ID number is 36Fa, and the scanned number will be 36F+A，“+” take one place
too.
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（d）Different type barcode digit see figure 5-1：
Sample Barcode Type Identify Code Number
Code 39 5～13
Code 128 5～18
Code 93 5～15
CODABAR 11
Figure 5-1
5.1.5 Check Waste Liquid, Sheath and Printer
(a) Sheath: put enough sheath in sheath barrel.

(b) Waste liquid: insert the waste liquid pipe into the waste liquid container and make sure there is
enough place for waste liquid.
(c) Printer: check whether the power supply wire and data cable for printer is correctly connected
and whether there is enough print paper.
5.2 Off-line Test
5.2.1 Plug in Power Supply & Register Software
(a) Turn on the computer, and the power supply switch for display;
(b) Turn on the power supply for printer and place the print paper;
(c) Turn on the power supply for FUS-100 urine sediment analyzer;
(d) Register FUS-100 urine sediment analyzer software and the instrument enter standby status.
Note: Switch on the power supply of FUS-100 urine sediment analyzer, and then login the
software.
5.2.2 Instrument Status Confirm
(a) Urine Sediment Analyzer, Urine Analyzer status confirm
Click “MONITOR” button in main menu display as figure 5-3:
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Figure 5-3
Urine Sediment Analyzer Mode: confirm “Urine Sediment Analyzer Mode” is on “Offline Mode”.
Urine Analyzer Status：make sure “Urine Analyzer Status” is on “Offline mode”.
LIS System: if LIS system is used, connect data cable, click” Check LIS”, make sure LIS system
is on “Online”.
Offline test can be conducted when above status are confirmed.
(b) Warning Tone for Alarm
The instrument has both alarm mark and warning tone during alarm. And you can select
button in main menu, enter interface select “on”, as shown in the

figure 5-4 :
Figure 5-4
So the instrument will give double remind for alarm.
(d)Focus: conduct once a day after booting according to User Manual.
(e)Calibration: conduct once a month according to User Manual.
(f)QC: conduct once a day after booting according to User Manual.
Note: the instrument should conduct focus, calibration and QC by turn, and conduct focus
and QC by turn when no need to do calibration.
5.2.3 Offline Sample Test
(a)Place the test tube with barcode outside and urine sample inside in the test tube rack, the
direction of the test tube should be straight.
(b)Place the test tube rack on the right of sample carrier, click “Start test” in “Sample Register”
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interface.
5.3 On-line Test

5.3.1 Plug in Power Supply & Register Software
(a) Turn on the computer, and the power supply switch for display;
(b) Turn on the power supply for printer and place the print paper;
(c) Turn on the power supply for FUS-100 urine sediment analyzer;
(d) Turn on the power supply switch for H-800 Urine Analyzer, the instrument enter standby mode
after self-checking;
(e) Register FUS-100 urine sediment analyzer software and the instrument enter standby status.
5.3.2 Instrument Status Confirm
(a)Connecting Status Confirm
Click in main menu, as figure 5-3 shows:

If Urine Analyzer status is “Offline”, connect the data wire of H-800 Urine Analyzer, click
“Connect”, make sure “Urine Analyzer Status” is “Online”, meanwhile, Urine Sediment Analyzer
should be “Online”, otherwise, online test can not be conducted.
(b)Alarm sound setting: same as 5.2.2 (b).
(c)Focus: conduct once a day after booting according to User Manual.
(d)Calibration: conduct once a month according to User Manual.
(e)QC: conduct once a day after booting according to User Manual.
Note: the instrument should conduct focus, calibration and QC by turn, and conduct focus
and QC by turn when no need to do calibration.

(f)H-800 Urine Analyzer Setting：click “Menu” in main interface, enter into menu setting, click
“Setting” to conduct the following setting:
♦ Printer: online test can not output print report, set the printer to be “All Off”.
♦ Unit：on online mode, urine analyzer unit can be international unit, conventional unit and plus
system. But it can not be DR-200 or Dr-800.
♦ Click , set ID “ON”, check whether “Test Tube Rack Load Mode”is “Online”,
contact DIRUI after-sale service personnel if it is “Offline”.
♦ Refer to Chapter Ⅲ of H-800 Urine Analyzer Manual for other setting.
5.3.3 Online Sample Test
(a) Place the tubes with barcode on the tube rack vertically.
(b) Place the tube rack on the right side of the H-800 urine analyzer sample carrier as figure 5-5
shows:
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Figure 5-5
Click the button in front of【start】on the screen and the instrument will feed in the tube
rack to test the sample one by one. The test result will be transmitted to the image working station
software, and display the sample NO., tube NO. and rack NO., meanwhile the current status will
be displayed in “Dry Chemistry” and “Online” line( appear in “Dry Chemistry” line means
the dry chemistry test has been finished, appear in “Online” column means the current
status is connected), as figure 5-6 shows:
Figure 5-6
The test tube rack will conduct feed-in after Urine Analyzer test has been finished, and the rack
will stop on the waiting area of connecting bridge.
Note: the number on the right upper corner of the urine analyzer screen, under the key
button is the reagent strips can be tested and the rack no. and tube no. of the sample to be
tested.
(c)After the test of urine analyzer, click “Sample Register” of main menu area of Urine Sediment
Image Workstation, click “Start Test” or press “Start” at the left upper corner of urine sediment
analyzer, the test tube rack will be transmitted to the sampling position of urine sediment analyzer
automatically, the following box will pop up meanwhile:
35
Figure 5-7
Input the number after “Code” of main screen before test in figure 5-7, click “OK”, urine sediment
analyzer will start test from the input sample NO.
（d）If connecting bridge failure happens, set the urine analyzer to be “Online” in urine sediment
image working station software(“Urine Analyzer Status” in figure 5-8). Set the “Test tube rack
transmitting mode” of urine analyzer to be “Off-line”. The data will be transmitted to the urine
sediment analyzer after each sample test. The test tube rack will be transmitted to the left of the
sample carrier of urine analyzer instead of connecting bridge.
Place the test tube rack on the right of auto-sampler of urine sediment analyzer after sample test.
Click of main menu, as figure 5-8 shows:
Figure 5-8
Click “OFF” after “Urine Sediment Analyzer Online Mode” in figure 5-8, the urine sediment
analyzer is on “Off-line” status, and click sample register of main menu of urine sediment
analyzer image working station software, click “Start Test” or press “Start” at the left upper corner
of urine sediment analyzer, the test tube rack will be transmitted to the sampling position of urine
sediment analyzer, figure 5-7 will pop up, input the start sample NO. for test, click OK, the urine
sediment analyzer will start test from the input sample NO.
36
5.4 Testing Process
5.4.1 Patient Information Editing
Note:
Patient information editing can be conducted before, during and after sample test(before
examine), in order to save time, it is better to be conducted during test.
Click sample register of main menu, as figure 5-9 shows:
Figure 5-9
Input sample number in “sam. No.” box and it can be selected through “previous sample” button
or “next sample” button.
Input pat. name, age, ID, bed No., detailed information and select patient sex, age, patient type,
sample type, date for sending, department for sending, doctor for sending, test doctor, dilution
times, etc. Memoni can be used in register.
Click “Modify” after editing, patient information will be displayed in browser.
Note: input patient name in patient information editing, otherwise, patient information
register can not be conducted.
Age: input age in input box, and select the age unit in the drop down list. This function can be set
in “System Management”, refer to User Manual for detail operation.
Patient type: select a right type in drop down list. This function should be set in “system
management” interface of main key area, refer to User Manual for detailed operation.
Sending department: select the department type for the sending sample. This function should be
37
set in “system management” interface of main key area, refer to User Manual for detail operation.
Sending doctor: select the doctor name for the sending sample. This function should be set in
“system management” interface of main key area, refer to User Manual for detail operation.
Sample type: select the suitable type. This function should be set in “system management”
interface of main key area, refer to User Manual for detail operation.
Dilution times: select the suitable type. This function should be set in “system management”
interface of main key area, refer to User Manual for detail operation.
5.4.2 Alarm Information Confirm
If failure occur during test, the software will prompt alarm remind and you can see the alarm mark
turning on the upper right side of screen.
Click the mark to display alarm code, alarm degree, alarm information, etc, as shown in
figure 5-10:
Figure 5-10
If the detail information need to be checked, click the information line, the detail alarm
information and the detail solution will be displayed, as figure 5-11 shows:
Figure 5-11
Refer to the failure solution, please contact DIRUI after-sales personnel if trouble can not be
solved.
5.4.3 Delete Alarm Information
38
Select the alarm information need to be deleted and click button under it. Click the
button under it and all the selected record will be deleted. Click button and all the
alarm record will be deleted.
5.5 Sample Review
If there is any sample need to be reviewed after first time test, select the sample number in “test
result” list, which is , click【review】button in figure 5-9, and shown in figure 5-12 :
Figure 5-12
Put the review samples on another tube rack and put the tube rack on the right side of the FUS-100
urine sediment analyzer sample carrier.
Re-select the sample number in figure 5-12 and click “Start Review” button for the review of the
sample.
The review result will be displayed in result column after sample review.
Note:
Only the test result of the day can be reviewed.
Dry chemistry result can not be reviewed, conduct re-test if review is needed.
Review sample test must be realized by clicking【start review】 button to send the order for
review.
5.6 Test Result

39
Double click the tested sample information to check the test result in “Test Result” list, as figure
5-11 shows:
Figure 5-11
The urine dry chemistry result is displayed on the right side of the interface.
The urine sediment result is displayed on the left side of the interface, each visual component and
standard valve value can be checked. The result within normal range will be green, the result
exceeds normal range will be red, and the exceed area will be red.
If there are to many visual components in the sample test result, click button on the
lower left place of the screen to review next page, and click button to read previous
page.
Click or button to get the review for other sample test

result.
（a）Each test image preview
Double click the button with cell mark for urine sediment result and the enlarged image will
display on the screen. If double click【SQEP】button will display as figure 5-12:
40
Figure 5-12
Click button or button on the right side of the screen to see the image for
visual component to check all the items on the right side.
Report Image Adding
Select the image need to be added in figure 5-12, right click the mouse,
will pop up, select “Send to the print report ”as figure 5-13 shows, left click the mouse to send the
image to the remark information column of the report.
Figure 5-13
Report Image Deleting
If the image send to the printing report is found to be wrong, select “Delete from printing report”
41
to delete the image, and add again.
(b) Image Manual Classification
If the image is not consistent with the test item name, the images can be reclassified, and the
operation will be as follows:
Select the image in Figure 5-12(inconsistent with name), press the correspond button on the right
of the screen to reclassify. Click “Result” and return to 5-11 interface, click “Accept” to save
and confirm the above modification.
Click button or button on the right side of the screen to check other test
item. Repeat the above steps until all the image need to be modified have been finished.
In the above procedure , if mistake in operation has been found, press【recover】button in figure
5-11 to cancel the operation and conduct reclassification.
The following items can do auto identification:
WBC, crystal, WBC Clumps, Bacteria, RBC, Yeast, Squamous Epithelial Cells, sperm,
Non-Squamous Epithelial Cells, Mucus, Hyaline Casts, Pathological Casts.
The following items can do manual identification:
Pathological Casts Pathological Casts Other

Calcium Oxalate
Hyaline Casts Kidney Epithelial Cells
Crystals
Pathological Crystals Granular Casts Pseudohyphae
Triple Phosphate Crystal Cellular Casts Mucus
Calcium Phosphate
Waxy Casts RBC Clumps
Crystals
Leucine Crystals Broad Casts Oval Fat Body
Amorphous Crystals Red Cell Casts Transitional Cells
Uric Acid Crystals White Cell Casts Budding Yeast
Calcium Carbonate
Epithelial Cell Casts Sperm
Crystals
Cystine Crystals Fat Casts Trichomonad
Tyrosine Crystals Pathological Casts Fat
5.7 Sample Examination

(a) Single Sample Examine
42
Select the sample and click for a single sample record information in Figure 5-7,
and the corresponding item will have status mark , sample examine has been finished.
(b)Lot Sample Examine
Click for lot samples record information in Figure 5-7, and input the sample
number for start and end samples in “sample number” box. Select the examiner in the drop-down
list as figure 5-14:
Figure 5-14
Click “OK”, close “Lot Examine”window, the examine record number will be displayed.
Note:
Examine can only be conducted for the sample with result and name.
The sample only with dry chemistry result can not be examined.
The start sample number for lot examine must be smaller or same as the end
sample number.
User with operator permission can only modify and delete the unexamined samples
of the day. User with administrator permission can modify the examined and
unexamined sample of the day.
5.8 Report Preview and Print

(a) Single sample Preview and Print
Click “Print” in figure 5-7 to preview the record need to be printed, as figure 5-16 shows:
43
Figure 5-16
Click “Close”to exit preview window. Click “ ” to print the report.

(b) Lot Sample Preview and Print
If lot report need to be printed, click “Lot Print”in figure 5-7, input the“Start No.”and “End No.”,
and click “Print”. If No. 15-18 sample result need to be printed, input sample No. as figure 5-17
shows.
Figure 5-17
Note: the start sample number must be smaller or same as the end sample number.
5.9 Result Delete
Click in figure 5-7 if any sample result need to be deleted, as figure 5-18 shows:
44
Figure 5-18
Input the start No. and the end No. of the sample need to be deleted in the input box of “Sample
No.”, click “OK” to delete the sample results.
Note：
The start sample No. must not be bigger than end sample No.
The testing sample can not be deleted.
Chapter Ⅵ Database Backup

Note:
Periodical database backup is recommended to avoid accidental data loss. Database backup and
recovery should be conducted under standby. Database recovery should be conducted with caution
for it may overwrite the current database.
Refer to User Manual for detail backup method.
45
Chapter Ⅶ Troubleshooting
Test sample when trouble exists may lead to inaccurate result.
If failure alarm is prompted during test, test must be carried out after failure is cleared.
This chapter is about the common failure and solution.
7.1 Main Unit Maintenance

7.1.1 Calibration Failed
1. Failure:
The value is not stable during calibration, fluctuates between 1300-1500.
2. Solution:
There are several solutions:
① The flow cell has been moved artificially. Adjust the flow cell position and the center. The user
can not uninstall the flow cell. It can only be moved by DIRUI maintenance personnel or the
operator trained by DIRUI engineer. Those untrained user uninstalling the flow cell will be
responsible for the result.
② If the calibration value is smaller than 800, mostly bubble exists in sample pump or sample
pump leaks. Check the leakage and replace the tubes. Check whether bubble exists in sample
pump if no leakage exists. Perfuse the liquid tubing if bubbles exist.
③ The concentration problem of the standard solution. The reason for that is the previous standard
solution concentration is too high, its following standard solution concentration is lower,
modification is neglected during sampling. Use the standard solution specified by DIRUI, its
storage should conform to the storage requirement of DIRUI.
7.1.2 Too Much Air Bubble
1. Failure
Software prompt “Too much bubble in sample or bloody urine”
2. Solution
Take down testing sample, let the probe aspirate air, the tube length inserted into the photocoupler
should be air. Use the smallest straight screw driver to adjust RP3 potentiometer, use multimeter to
adjust to Voltage and test the voltage of RP3 potentiometer, adjust the voltage value to
2.95V±0.5V.
Note：
The tube length inserted into the photocoupler is set to test the probe air or bloody urine during
test. Unplug the tube length, the probe will not test air and bloody urine, the software will not
prompt alarm. This position should be dealt with caution during maintenance.
7.1.3 M5、M4、M3 Motor Error
1．Failure
46
“M5 Motor Zero Position Optocoupler Error” or “M4 Motor Zero Position Optocoupler Error” or
“M3 Motor Zero Position Optocoupler Error” is prompted during software test.
2. Solution
Dust may accumulated in the instrument after long time using. Alarm will be prompted, wipe the
optocoupler with cotton cloth in this condition. If the same optocoupler is wiped for continuous 2
times, contact the after-sale service personnel at the 2nd time.
Note: reset is needed after wiping.
7.1.4 Electric Level Error
1．Failure
“Vertical motor electric error”or “Horizontal motor electric error” is prompted during test.
2. Solution
Power cord YJ1 or TJ1 of sampling system may be touched during packaging, transportation or
debugging. Replug YJ1 into TJ1.
7.2 Mechanical System Maintenance

7.2.1 Counter in Sample Carrier
1．Failure
① Counter error during test, for example: two test tube racks have passed, but the counter display
less than 2 or more than 2 test tube racks.
② The first test tube rack enters into test area during test, alarm is prompted, the test tube rack is
not in place is displayed.
③ The test tube rack is removed displayed during test.
2. Solution
⑴There are 2 solutions for Failure ①:
ⅠArtificial operation error, no test tube rack on sample carrier, during artificial operation, the
system will prompt counter error, click reset in operation software.
ⅡMicro switch damage or position error of micro switch, conducted reset, touch the lever in front
of micro switch, imitate test tube rack passing through artificially to check the counter, it the value
is correct, the micro switch is not damaged, the reason may be the micro switch position is
incorrect, it should be adjusted. Let the micro switch touch the lever at free condition, and conduct
reset.
⑵ Failure ② is the position problem of micro switch, adjust the position of micro switch, conduct
reset.
⑶ Failure③ is artificial problem，take the test tube rack down along the moving direction,
conduct reset.
Note:
If the lever is not lifted, the lever may be rusted, uninstall the lever assembly, wipe it with alcohol.
Reset is needed after above failures solved.
47
7.3 Optical System Maintenance

7.3.1 Focus Motor
1．Failure
Focus is needed if the FUS-100 Urine Sediment Analyzer has not been used for a long time. Focus
motor error is prompted.
2. Solution
Check the slipway in front of focus motor. Sludge or rust may occur. Move push and pull the
slipway for several times until it can move back automatically.
Note:
As the optical system is sophisticated component, no rust, liquid spill or impurity is allowed on the
slipway, keep the surface clean.
7.3.2 Focus Failure
1．Failure
The system prompts “Focus Failed”
2. Solution
① Adjust grey value, adjust the power voltage of floodlight. The light will be bright when the
voltage is turned up. The light will be dark when the voltage is turned down.
② Focus motor zero position optocoupler position, adjust the focus to the running line. The detail
adjustment method will be trained by optical engineer.
③ Focus concentration is too low, replace focus.
7.3.3 Optical Fiber Sensor Error
1．Failure
“Optical fiber sensor error” is prompted during test.
2. Solution
Under normal condition, the red indicator on the optical fiber sensor is not lighted when no tube
passing through. If it is lighted, the indicator is abnormal. Dust may exists on reflective mirror
which is at the opposite side of optical fiber sensor, remove the dust. Or maybe the bad connection
between the probehead of optical fiber sensor and sensor port. Use specified scissors to recut and
insert it(it must be conducted by or under the guidance of DIRUI professional, otherwise ,the user
will be responsible for the result).
Readjust the optical fiber sensor value if failure is not solved by above solution:
Place the test tube with water(4/5 of the tube) in the test tube rack. Push the test tube rack into the
sample collecting area, place the test tube at the position of probe sampling to check the optical
fiber sensor value, record the value, and push back the rack, record the value again, plus the two
value and divided by 2, this value will be the set value for optical fiber sensor. Press “△”or“▽”on
optical fiber sensor(these is used to adjust the value).When the screen flashes, press “△”or“▽”
longer to adjust the value. The optical sensor counter will recover to working status after certain
times button releasing.
Note: the test tube used in test must be glass test tube.
7.4 Liquid Tubing System Maintenance
48
7.4.1 Sheath Insufficient Alarm

1．Failure
“Sheath Insufficient” is prompted.
2. Solution
① If the alarm is prompted at the first time liquid tubing fill, click “OK”to perfuse tubing.
② If it is not the first time, the following solutions are offered:
ⅠLiquid level sensor failure, use multimeter to check the wire connection and whether the float in
liquid level sensor is blocked, conduct adjustment according to the test result.
ⅡBubble exists in sheath, the reason for that is the misoperation of the user: replace sheath during
sheath filling, or the sheath leve is lower than the tubing. The alarm can be neglected at this
condition, click “OK” to continue.
Ⅲ Impurity exists in diaphragm pump P4, replace or maintain(open it and remove the impurity) it.
Note：
Diaphragm pump P4 can only be maintained by DIRUI personnel or returned to the manufacturer.
7.4.2 Waste Tubing Block

1．Failure
“Waste Tubing Block” is prompted
2. Solution
Check the outer waste liquid tubing connection, whether liquid tubing is squeezed;
Open the instrument cover to check whether liquid exists in waste chamber, if it does, P2
diaphragm pump is still working, the waste has not been discharged, suggesting P2 diaphragm
pump is blocked, replace or maintain it; if it doesn’t, pour water into waste chamber, if it is
discharged smoothly, liquid level sensor probe short circuit may occur, the two probes may get
close to each other, or the probe touch the waste chamber wall, separate the probe artificially at
this condition. Or liquid in liquid level sensor spill occur, leading to short circuit, wipe it with
alcohol to solve the problem.
7.4.3 Syringe Pump Make Sound
Emargination
major grove
ball bearing
F604
49
1．Failure
For the long working period of the instrument, the 3 syringe pump at the back may make sound
during test.
2. Solution
Take down the emargination major grove ball bearing.
7.4.4 Small Value

1．Failure
The test value is quite small during test.
2. Solution
Insufficient sample volume leading to air aspiration, or the sample pump did not push the liquid
into the flow cell , or the pushed volume is insufficient. If it is the former one, add more sample, if
it is the latter one, check the 1.5 ml sample pump, or tighten the connections, replace the damaged
connectors, or replace the damaged syringe pump.
7.4.5 Air Pump Keep on Working
1．Failure
Under standby(or during work), P1 air pump is discontinuit working, P1 air pump begin to work
when the air pressure is lower than 0.1KPa, it stop working when the air pressure is higher than
0.1KPa, the air pump keep on working and the P1 pump motor is hot.
2. Solution
The reason for above failure is P1 air pump loop leakage exists, check the loop to find the leakage.
7.5 Software System Maintenance

7.5.1 Installation（FUS100 connect with H800）
1．Failure
When connecting FUS-100 and H-800, the system prompts “Please set correct dry chemistry
connecting agreement”.
3. Solution
Enter in to manufacturer password of H-800(password is 122), set GLV2 to be 0002, save after set
and connect, if “Please set correct dry chemistry connecting agreement” is still prompted,
suggesting H-800 program is old version, upgrading is needed.
Note：
If the failure is not solved by above solution, contact R&D dept.
50

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Service Manual of FUS-100 Urine Sediment Analyzer

FUS-100 Urine Sediment Analyzer

5.2 Off-line Test ...................................................................................................................... 32

1.1 Urine Sediment Analyzer Overview

1.2 Test Principle

1.3 System Principle Diagram

①“Start” Button ②Key Switch ③Sample Carrier

1.3.1.2 Rear View

①RS-232 ② Sheath Inlet ③Waste Outlet ④Power

⑤Power Switch ⑥Camera Port ⑦Fan

1.3.1.3 Right Side View

1.3.2 Air and Liquid Principle Diagram

1.3.3 Electric Principle Diagram

Figure 1-4 General Circuit

Figure 1-5 Main Control Board Circuit（1）

Figure 1-6 Main Control Board Circuit（2）

Figure 1-7 Main Control Board Circuit（3）

1.3.4 Optics Principles Diagram

1. Pulse Xenon Lamp 2. Illuminator 3. Aperture Stop

4. Green Filter 5. Condenser 6. Object Plane

7. Objective Lens 8. Attachment Objective 9. CCD Camera

1.4 Test Item and Image

Red Blood Cell RBC

White Blood Cell WBC

White Blood Cell

Hyaline Cast HYAL

Pathological Cast UNCC

The prompts to pay attention, otherwise, may result in personal injury.

Biology pollution mark

Only for in vitro diagnosis use

Calculate allowed mark

2.1 Installation Instruction

2.2 External Device Connection

2.2.1 Offline Mode

2.2.2 Online Mode

2.2.3 Software Installation

Chapter Ⅲ Focus and Calibration

1. Plastic test tube or plastic pipette can not be used in focus.

3. Daily focus should be conducted after booting.

♦ Plastic test tube or plastic pipette can not be used in calibration.

♦ Please use the Standard Solution specified by DIRUI.

3-2 for its display:

QC purpose is to make sure test result accuracy and repeatability.

Note：Operate in strict accordance with the steps.

4.2 QC Register and QC Setting

Click , , as figure 4-1 shows:

Click “Yes”, the selected QC item will be deleted.

Click “QC Setting” in figure 4-1, as figure 4-3 shows:

Select the commonly used alarm range, click , as Figure 4- 4 shows:

Click “Yes” to save the new QC range.

(b)QC Test Note

♦ Plastic test tube or plastic pipette can not be used in QC.

♦ Please use the Control specified by DIRUI.

♦ Click “QC” in figure 4-3, as figure 4-5 shows:

No.( ) need to be tested in QC register interface. Click “QC”, the sample

4.4.1 QC Result Inquiry

Click “Inquire” in figure 4-6, as figure 4-7 shows:

(a) Inquire through lot number

click button, shown as figure 4-8:

Click button for the print of the QC result.

(b) Inquire through QC name

figure 4-7 and click button, shown as 4-10:

（c）Inquire through status