Pati Hydrolysis (Amylum)

a. Test Tube 1
2 ml of pati (amylum) solution

1. Added 2 ml of HCl 3M
2. Heated in steam bath
3. Cooled in room temperature
4. Added 3 ml of NaOH 3M
5. Divided into 2 test tube

1a 1b
6. Iodine test 6. Added 5 ml of
Benedict reagent

Result Result

b. Test Tube 2
2 ml of pati (amylum) solution

1. Added 2 ml of water
2. Heated in steam bath
3. Cooled in room temperature
4. Added 3 ml of water
5. Divided into 2 test tube

2a 2b
6. Iodine test 6. Added 5 ml of
Benedict reagent

Result Result
c. Test Tube 3

2 ml of pati (amylum) solution

1. Added 2 ml of water
2. Let it in room temperature
3. Added 3 ml of water

3a 3b
4. Iodine test 4. Added 5 ml of
Benedict reagent

Result Result