D.

APPARATUS AND CHEMICALS

1. Apparatus
a. Plate 1 piece
b. Drop pipette 7 pieces
c. Test tube 12 pieces
d. Wood clamp 2 pieces
e. Rack test tube 3 pieces
f. Beaker 100 mL 1 piece
g. Beaker 500 mL 1 piece
h. Spray bottle 1 piece
i. Stirr bar 1 piece
j. Dropper 5 pieces
k. Stopwatch 1 piece
l. Autoclave 1 piece
m. Rough cloth 1 piece
n. Smooth cloth 1 piece
o. Burner 3 pieces
p.Graduated cylinder 1 piece
q. Measuring glass 1 piece
2. Chemicals
a. Bread yeast (Saccharomyces cerevisiae)
b. Tape yeast (Aspergillus)
c. Tempe yeast ( Rhizopus oligosporus)
d. Starch 1% solution ( C6H12O5 )
e. Glucose ( C6H12O6 )
f. Fructose 5% ( C6H12O6 )
g. Galactose 5% ( C6H12O6 )
h. Iodine 0.1% ( I2 )
i. Reagent Benedict/ Fehling
j. Reagent tollens
 k. Aquadest ( H2O)
a. Tissue
b. Aluminium foil
c. Label
E. WORD PROCEDURE

F. OBSERVATION RESULT
1. Hydrolysis of strach
No Activity Result

1 1/10 bread yeast + 25 ml H2O ( colorless) Tirbid solution

3 drops amylum + 3 drops of bread yeast White turbid

suspension

a. Plate 2 ( 3 minutes )

turbid solution + drops of iod 0,1% (yellow) Colorless solution

b.Plate 3 ( 4 minutes)

turbid solution + drops of iod 0,1% (yellow) Colorless solution

c. Plate 4 ( 6 minutes)

turbid solution + drops of iod 0,1% (yellow) colorless solution

2 1/10 tapai bread yeast + 25 mL aquadest Colorless

a. Plate ( 3 minute)

turbid solution + drops of iod 0,1% (yellow) Purple solution

b. Plate 5 ( 6 minute)

turbid solution + drops of iod 0,1% (yellow) Purple solution

c. Plate 6 ( 9 minute)
 turbid solution + drops of iod 0,1% (yellow) Purple solution

3 1/10 tempe bread yeast + 25 mL aquadest Turbid solution

a. Plate 7 (3 minute)

turbid solution + drops of iod 0,1% (yellow) Purple solution

b. Plate 8 (6 minute)

turbid solution + drops of iod 0,1% (yellow) Purple solution

c. Plate 9 (9 minute)

turbid solution + drops of iod 0,1% (yellow) Purple solution

4 Blue solution
Plate 1;3 drops of amylum + 3 drops of H2O
+ 2 drops of iod 0,1 %

2. Alcohol Fermentation
No Activity Result

1 5 mL of starch 1% was put into tube 1, 2, 3 Turbid solution

5 mL of glucose 5% wa put into tube 4, 5, 6 Colorless

5 mL of fructose 5% was put intotube 7, 8 9 Yellowish

5 mL of Galactose 5% was put into tube 10, Colorless

11, 12

2 All the tube was closed by cotton and Hot the solution for the
sterilization in autoclave until 10 minutes ( T= all tube
110o C)

3 All tube was cobled in room temperature There are bubble

4 a. In tube 1 ( starch . glucose, fructose, and No previous color

 galactose) was added 2 ml of bread yeast change

b. In tube 2 ( starch . glucose, fructose, and No previous color

galactose) was added 2 ml of bread yeast change

c. In tube 3 ( starch . glucose, fructose, and No previous color

galactose) was added 2 ml of bread yeast change

5 After incubation at room temperature until 24

hours

a. Starch

Tube 1 ( bread) There are CO2 gas and

smell alcohol

Tube 2 ( tapai) There are CO2 gas and

smell alcohol

There are not CO2 gas

Tube 3 ( tempe)
and smell alcohol

b. Glucose
There are CO2 gas and
Tube 1 (bread ) smell alcohol

There are CO2 gas and

Tube 2 (tapai) smell alcohol

There are CO2 gas and

smell alcohol
Tube 3 (tempe)

There are not CO2 gas

c.Fructose
and there is smell
Tube 1 (bread )
There are not CO2 gas
 and there is smell

Tube 2 (tapai) There are not CO2 gas

and there is smell

Tube 3 (tempe)
There are not CO2 gas

A. Galactose and there is smell

Tube 1 (bread ) There are not CO2 gas

and there is smell

Tube 2 (tapai) There are not CO2 gas

and there is smell

Tube 3 (tempe)

6 For tube that contain starch benedict test

Tube 1 (bread) Yellow to orange

solution

Orange Solution
Tube 2 ( tapai)
Orange solution
Tube 3 (tempe)

For tube that contain starch tollens test it

There is silver mirror
Tube 1 (bread)
solution

There is silver mirror

Tube 2 (tapai) solution
 There is silver mirror
solution
Tube 3 (tempe)