COCO CORPORATION FREE FATTY ACIDS

DEPARTMENT DOC NO.: VERSION NO.

ANALYTICAL LAB CC-TM-44 DRAFT
DATE: PAGE:
MAY 2020 Page 1 of 2

1 SCOPE

2 DEFINITIONS

3 PRINCIPLE

4 REAGENTS /APPARATUS /EQUIPMENTS

 Fat extraction device (Soxhlet or other suitable type)
 Graduated cylinder (50mL)
 Toluene-alcohol-phenolphthalein solution
0.02%. To 1 L toluene add 1 L alcohol and 0.4 g phenolphthalein.
 Alcohol-phenolphthalein solution
0.04%. To 1 L alcohol add 0.4 g phenolphthalein.
 0.0178M KOH Solution
 Petroleum ether
 0.5% K2Cr2O7 solution

5 REFERENCE DOCUMENTS
AOAC Official Test Method 939.05 Fat Acidity- Grains

6 HAZARDS /SAFETY /PPE

 Gloves

7 PROCEDURE
7.1 If test sample is moist, dry at temperature of around 100°C at oven just long enough
to remove excess moisture.
7.2 Extract 10 ± 0.01 g test portion with petroleum ether around 16h in extractor.
7.3 Start extraction as soon as possible and never let ground test sample remain
overnight.
7.4 Completely evaporate solvent from extract on steam bath.
7.5 Dissolve residue in extraction flask with 50 mL toluene–alcohol–phenolphthalein
solution.
7.6 Titrate with standard KOH solution to distinct pink, or in case of yellow solution to
orange-pink.
7.7 If emulsion forms during titration, dispel by adding second 50 mL portion of toluene–
alcohol–phenolphthalein solution.
7.8 End point should match color of solution made by adding 2.5 mL 0.01% KMnO4
solution to 50 mL K2Cr2O7 solution of proper strength to match color of original

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ANALYTICAL LAB (insert name here)

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COCO CORPORATION FREE FATTY ACIDS
DEPARTMENT DOC NO.: VERSION NO.
ANALYTICAL LAB CC-TM-44 DRAFT
DATE: PAGE:
MAY 2020 Page 2 of 2

solution being titrated. (Add 0.5% K2Cr2O7 solution dropwise to 50 mL H2O until
color matches. Then add 2.5 mL 0.01% KMnO4 solution.)
7.9 Make blank titration on 50 mL toluene–alcohol–phenolphthalein solution and subtract
this value from titration value of test sample. If additional 50mLportion toluene–
alcohol–phenolphthalein solution was added, double blank titration.
7.10 Report fat acidity as mg KOH required to neutralize free fatty acids from 100 g grain
(dry basis).

8 CALCULATION

Fat acidity = 10 x (titration - blank)

9 TEST REPORT
Proceed with Test Report Form.

10 DISPOSAL OF MATERIALS

PREPARED BY : APPROVED BY :

ANALYTICAL LAB (insert name here)

MANAGER