COCO CORPORATION PEROXIDE VALUE

DEPARTMENT DOC NO.: VERSION NO.

ANALYTICAL LAB CC-TM-45 DRAFT
DATE: PAGE:
MAY 2020 Page 1 of 2

1 SCOPE

2 DEFINITIONS

3 PRINCIPLE

4 REAGENTS /APPARATUS /EQUIPMENTS

 Conical flask
 Graduated cylinder (50mL)
 Pipet (1mL)
 Acetic acid – chloroform
Mix 3 volumes CH3COOH With 2 volumes CHCl3
 Saturated KI Solution
 0.1M Na2S2O4 solution
 0.01M Na2S2O4 solution

5 REFERENCE DOCUMENTS
AOAC Official Test Method 965.33

6 HAZARDS /SAFETY /PPE

 Gloves

7 PROCEDURE
7.1 Weigh 5.00±0.05 g test portion into 250 mL Conical flask.
7.2 Add 30 mL Acetic acid – chloroform and swirl to dissolve
7.3 Add 0.5 mL saturated KI solution
7.4 Let stand with occasional shaking 1 min and add 30 mL H2O
7.5 Slowly tirate with 0.1M Na2S2O3 with vigorous shaking until yellow is almost gone.
7.6 Add aroun 0.5 mL 1% starch solution.
7.7 Continue tiration,shaking vigorously to release all I2 from ChCl3 layer, until blue just
disappears.
7.8 If <0.5 mL 0.1M Na2S2O3 is used, repeat determination with 0.01M Na2S2O3
7.9 Conduct blank detemination(must be ≤0.1 mL Na2S2O3).
7.10 Subtract from test portion titration.

8 CALCULATION

Peroxide value(milliequivalent peroxide/kg oil or fat) = S x M x 1000

g sample

PREPARED BY : APPROVED BY :

ANALYTICAL LAB (insert name here)

MANAGER
COCO CORPORATION PEROXIDE VALUE
DEPARTMENT DOC NO.: VERSION NO.
ANALYTICAL LAB CC-TM-45 DRAFT
DATE: PAGE:
MAY 2020 Page 2 of 2

where,
S=mL Na2S2O3 (blank corrected)
M = molarity Na2S2O3 solution

9 TEST REPORT
Proceed with Test Report Form.

10 DISPOSAL OF MATERIALS

PREPARED BY : APPROVED BY :

ANALYTICAL LAB (insert name here)

MANAGER