Cholesterol – major lipids in the body// to maintain cellular struct and integ
Precursor of steroid hormones// assoc w/ CHD// unsaturated steroid alcohol
70% is esterified form (cholesteryl ester)// chole transported by lipoprots
RGT --- phosphate buffer (6.5), 4-aminophenazone, phenol, peroxidase (PO)
Cholesterol esterase(CE), cholesterol oxidase(CO), Na Azide (NaA)
STD = 200mg/dl or 5.17mmol/L
Chole is determnd after enzymatic hydrolysis and oxidation. Enzy (CE,CO PO)
Quinoneimine is formed from H2O2 and 4amino in pres in phenol and perox
500nm , 1cm , 37C, against rgt blank, semi-micro (0.01ml)
HDL-C good chol – functions to remove excess chole from cells //
Higher protein than lipid// major component =apo A-I
Ezyme = goods buffer (6.6), NaCl, CE, CO, catalase, ascorbate oxidase, N-HDAOS
Substrate = peroxidase, 4-amnantipyrn, Gud’s (7.0) preservative, detergent, NaA
STD= check the bottle container
HDL is measured direct homogenous assay. 1st step = elim chylo, vLDL, LDL using
CE,CO, catalase. 2nd step= HDL + chromogen and peroxidase
593nm, 1cm, 37Cm agnst rgt blnk, semi-micro(0.01) enzyme(0.75) substrat(0.25)
Trigly = 3FA to 1glycerol (esterbond)// solid at RT, animal source =liquid
Assoc with CHD and hormonal abnormties (pancreas, adrenal, pituitary)
Assoc of chd in often with decreased HDLC
RGT = PIPES (7.5) 4chlorophenol, 4-aminoantipyrine, Mg+, ATP, lipases, PO,
Glycereol kinase, glycerol-3-phosphate oxidase
Std= tri = 200mg/dl or 2.28mmol/l
500nm,1cm,37C, against rgt blnk (semimicro)
Calculation LDL = LDL = TC – HDL – TG/(5mg/dl) or TG/(2.175mmol/L)
Most protein synth = liver// total protein = assessment synth func of liver.
Prot def = malabsorption, malnutrition. Most common req. = TP and Albumin
RGT = NaOH, KNa tartrate, CuSulfate, KI
STD protein = 8g/dl or 80g/L, NaA
Peptide bonds chelated by Cu ion = violet colored comp// biuret’s rxn
520-580nm 1cm 37C against Rgt blank
Albumin= major rpot in plasma/// assessing liver func// other func= maintain
Oncotic pressure, pH and binding with various subs (Thyroid H, bilib, Iron, FA)
Rgt = citrate (4.2) bromcresol green STD = 4g/dL or 40g/L NaA
578nm, 1cm, 37C, against rgt blnk, semimicro
Cholesterol – major lipids in the body// to maintain cellular struct and integ
Precursor of steroid hormones// assoc w/ CHD// unsaturated steroid alcohol
70% is esterified form (cholesteryl ester)// chole transported by lipoprots
RGT --- phosphate buffer (6.5), 4-aminophenazone, phenol, peroxidase (PO)
Cholesterol esterase(CE), cholesterol oxidase(CO), Na Azide (NaA)
STD = 200mg/dl or 5.17mmol/L
Chole is determnd after enzymatic hydrolysis and oxidation. Enzy (CE,CO PO)
Quinoneimine is formed from H2O2 and 4amino in pres in phenol and perox
500nm , 1cm , 37C, against rgt blank, semi-micro (0.01ml)
HDL-C good chol – functions to remove excess chole from cells //
Higher protein than lipid// major component =apo A-I
Ezyme = goods buffer (6.6), NaCl, CE, CO, catalase, ascorbate oxidase, N-HDAOS
Substrate = peroxidase, 4-amnantipyrn, Gud’s (7.0) preservative, detergent, NaA
STD= check the bottle container
HDL is measured direct homogenous assay. 1st step = elim chylo, vLDL, LDL using
CE,CO, catalase. 2nd step= HDL + chromogen and peroxidase
593nm, 1cm, 37Cm agnst rgt blnk, semi-micro(0.01) enzyme(0.75) substrat(0.25)
Trigly = 3FA to 1glycerol (esterbond)// solid at RT, animal source =liquid
Assoc with CHD and hormonal abnormties (pancreas, adrenal, pituitary)
Assoc of chd in often with decreased HDLC
RGT = PIPES (7.5) 4chlorophenol, 4-aminoantipyrine, Mg+, ATP, lipases, PO,
Glycereol kinase, glycerol-3-phosphate oxidase
Std= tri = 200mg/dl or 2.28mmol/l
500nm,1cm,37C, against rgt blnk (semimicro)
Calculation LDL = LDL = TC – HDL – TG/(5mg/dl) or TG/(2.175mmol/L)
Most protein synth = liver// total protein = assessment synth func of liver.
Prot def = malabsorption, malnutrition. Most common req. = TP and Albumin
RGT = NaOH, KNa tartrate, CuSulfate, KI
STD protein = 8g/dl or 80g/L, NaA
Peptide bonds chelated by Cu ion = violet colored comp// biuret’s rxn
520-580nm 1cm 37C against Rgt blank
Albumin= major rpot in plasma/// assessing liver func// other func= maintain
Oncotic pressure, pH and binding with various subs (Thyroid H, bilib, Iron, FA)
Rgt = citrate (4.2) bromcresol green STD = 4g/dL or 40g/L NaA
578nm, 1cm, 37C, against rgt blnk, semimicro
Cholesterol – major lipids in the body// to maintain cellular struct and integ
Precursor of steroid hormones// assoc w/ CHD// unsaturated steroid alcohol
70% is esterified form (cholesteryl ester)// chole transported by lipoprots
RGT --- phosphate buffer (6.5), 4-aminophenazone, phenol, peroxidase (PO)
Cholesterol esterase(CE), cholesterol oxidase(CO), Na Azide (NaA)
STD = 200mg/dl or 5.17mmol/L
Chole is determnd after enzymatic hydrolysis and oxidation. Enzy (CE,CO PO)
Quinoneimine is formed from H2O2 and 4amino in pres in phenol and perox
500nm , 1cm , 37C, against rgt blank, semi-micro (0.01ml)
HDL-C good chol – functions to remove excess chole from cells //
Higher protein than lipid// major component =apo A-I
Ezyme = goods buffer (6.6), NaCl, CE, CO, catalase, ascorbate oxidase, N-HDAOS
Substrate = peroxidase, 4-amnantipyrn, Gud’s (7.0) preservative, detergent, NaA
STD= check the bottle container
HDL is measured direct homogenous assay. 1st step = elim chylo, vLDL, LDL using
CE,CO, catalase. 2nd step= HDL + chromogen and peroxidase
593nm, 1cm, 37Cm agnst rgt blnk, semi-micro(0.01) enzyme(0.75) substrat(0.25)
Trigly = 3FA to 1glycerol (esterbond)// solid at RT, animal source =liquid
Assoc with CHD and hormonal abnormties (pancreas, adrenal, pituitary)
Assoc of chd in often with decreased HDLC
RGT = PIPES (7.5) 4chlorophenol, 4-aminoantipyrine, Mg+, ATP, lipases, PO,
Glycereol kinase, glycerol-3-phosphate oxidase
Std= tri = 200mg/dl or 2.28mmol/l
500nm,1cm,37C, against rgt blnk (semimicro)
Calculation LDL = LDL = TC – HDL – TG/(5mg/dl) or TG/(2.175mmol/L)
Most protein synth = liver// total protein = assessment synth func of liver.
Prot def = malabsorption, malnutrition. Most common req. = TP and Albumin
RGT = NaOH, KNa tartrate, CuSulfate, KI
STD protein = 8g/dl or 80g/L, NaA
Peptide bonds chelated by Cu ion = violet colored comp// biuret’s rxn
520-580nm 1cm 37C against Rgt blank
Albumin= major rpot in plasma/// assessing liver func// other func= maintain
Oncotic pressure, pH and binding with various subs (Thyroid H, bilib, Iron, FA)
Rgt = citrate (4.2) bromcresol green STD = 4g/dL or 40g/L NaA
578nm, 1cm, 37C, against rgt blnk, semimicro
Cholesterol – major lipids in the body// to maintain cellular struct and integ
Precursor of steroid hormones// assoc w/ CHD// unsaturated steroid alcohol
70% is esterified form (cholesteryl ester)// chole transported by lipoprots
RGT --- phosphate buffer (6.5), 4-aminophenazone, phenol, peroxidase (PO)
Cholesterol esterase(CE), cholesterol oxidase(CO), Na Azide (NaA)
STD = 200mg/dl or 5.17mmol/L
Chole is determnd after enzymatic hydrolysis and oxidation. Enzy (CE,CO PO)
Quinoneimine is formed from H2O2 and 4amino in pres in phenol and perox
500nm , 1cm , 37C, against rgt blank, semi-micro (0.01ml)
HDL-C good chol – functions to remove excess chole from cells //
Higher protein than lipid// major component =apo A-I
Ezyme = goods buffer (6.6), NaCl, CE, CO, catalase, ascorbate oxidase, N-HDAOS
Substrate = peroxidase, 4-amnantipyrn, Gud’s (7.0) preservative, detergent, NaA
STD= check the bottle container
HDL is measured direct homogenous assay. 1st step = elim chylo, vLDL, LDL using
CE,CO, catalase. 2nd step= HDL + chromogen and peroxidase
593nm, 1cm, 37Cm agnst rgt blnk, semi-micro(0.01) enzyme(0.75) substrat(0.25)
Trigly = 3FA to 1glycerol (esterbond)// solid at RT, animal source =liquid
Assoc with CHD and hormonal abnormties (pancreas, adrenal, pituitary)
Assoc of chd in often with decreased HDLC
RGT = PIPES (7.5) 4chlorophenol, 4-aminoantipyrine, Mg+, ATP, lipases, PO,
Glycereol kinase, glycerol-3-phosphate oxidase
Std= tri = 200mg/dl or 2.28mmol/l
500nm,1cm,37C, against rgt blnk (semimicro)
Calculation LDL = LDL = TC – HDL – TG/(5mg/dl) or TG/(2.175mmol/L)
Most protein synth = liver// total protein = assessment synth func of liver.
Prot def = malabsorption, malnutrition. Most common req. = TP and Albumin
RGT = NaOH, KNa tartrate, CuSulfate, KI
STD protein = 8g/dl or 80g/L, NaA
Peptide bonds chelated by Cu ion = violet colored comp// biuret’s rxn
520-580nm 1cm 37C against Rgt blank
Albumin= major rpot in plasma/// assessing liver func// other func= maintain
Oncotic pressure, pH and binding with various subs (Thyroid H, bilib, Iron, FA)
Rgt = citrate (4.2) bromcresol green STD = 4g/dL or 40g/L NaA
578nm, 1cm, 37C, against rgt blnk, semimicro