Principle of Biochemical Engineering

Course Code: CHEG 213

Course outline

1. Basic of microbiology: [4 hour]

1.2 Biophysics and cell doctrine,
1.3 The structure of cell: Prokaryotic and Eukaryotic cell
1.3 Important types of cell types: Bacteria, Fungi, Algae and Protozoa, Animal and plant cell
1.4 Bacterial growth and Nutritional requirement
2. Chemicals for Life [ 8 hours]
2.1 Lipids: Fatty acids and related Lipids, Fat soluble vitamins, steroids and other lipids
2.2 Sugar and Polysaccharides: D-glucose and other monosaccharides, disaccharides to
Polysaccharides and cellulose.
2.3 From Nucleotides to RNA and DNA: Building blocks, and energy carrier and coenzyme,
Biological information storage: DNA and RNA
2.4 Amino, Peptides and Protein: Amino acid building blocks and polypeptides, protein structure,
primary structure, secondary and tertiary structure, quaternary structure and biological
regulation
2.5 Hybrid Biochemical : peptidoglycan and lipopolysaccharides, antibodies and other
glycoproteins
3. The Kinetics of Enzyme-Catalyzed Reactions [10 hours]
3.1. Introduction, classification and nomenclature of enzymes, enzyme substrate complex and
enzyme action
3.2. Michaelis-Menten Kinetics, evaluation of parameters in the Michaelis-Menten equation,
Lineweaver-Burk plot, Eadie-Hofstee plot, Hanes-Woolf plot
3.3. Kinetics of reversible reaction, two substrate reactions and cofactor activation.
3.4. Enzyme Inhibition, Inhibited enzyme kinetics, effect of pH on enzyme kinetic in solution,
enzyme reaction rates and temperature
3.2 Enzyme deactivation, Immobilized enzyme technology and Industrial application of enzyme.
4. Metabolic Stoichiometry and Energetic [8 hrs]
4.1 Introduction
4.2. Metabolic reaction coupling: ATP and other phosphate compounds, oxidation and reduction:
coupling via NAD
4.3. Carbon Catabolism: Embeded-Mayerhof-Parnad Pathway, Other carbohydrate catabolism
pathways
4.4. Respiration: TCA cycle and respiratory chain
4.5. Photosynthesis, Biosynthesis: synthesis of small molecules, macromolecule synthesis
4.6. Transport across cell membrane: passive and facilitated diffusion, active transport
5. Molecular Genetics and Control System: [10 hours]
5.1. The process of Gene expression,
5.2. Split Genes and mRNA modification in Eucaroytes
5.3 Posttranslational modification of protein
5.4 Induction and Repression: Control of protein synthesis
5.5. DNA replication and mutation
5.6 Overview of Information flow in the cell.
5.7. Recombinant DNA technology: Polymerase chain reaction, gene cloning, vectors for
Escherichia coli, Characterization of Cloned DNAs.

6. Kinetic of Substrate utilization, product formation and biomass production in cell culture.
[5 hours]
6.1 Ideal Reactor for Kinetic Measurement: Ideal batch reaction, the ideal continuous –flow
stirred tank reaction (CSTR)
6.2 Kinetics of Balanced Growth: Monod Growth Kinetics, Kinetic Implication of endogenous
and Maintenance metabolism
6.3. Other form of growth kinetics, Environmental effect on growth kinetics

7. Transport Phenomenon in Bioprocess system [3 hours]

7.1. Gas-Liquid Transfer in Cellular system: Basic mass transfer concepts, Rates of metabolic
oxygen utilization
7.2. Determination of Oxygen Transfer Rates: Measurement of Kla’ Using Gas –Liquid Reaction
Text Book:
1. Biochemical Engineering Fundamental, 2nd Edition by JAMES E. BAILEY, DAVID F. OLLIS.
McGraw-Hill INTERNATIONAL.
Reference book
1. Lehninger Principle of Biochemistry, fourth edition by David L Nelson and Michel M. Cox.
2. Introduction to Biochemical Engineering by DG RAO, The McGRAW-HILL
3. Bioprocess Engineering, 2nd edtion by Michael L . Shuler and Fikret Kargi