Professional Documents
Culture Documents
1. Amino acids
a. have more than one a-carbon atom
b. cannot participate in covalent bond formation.
c. Only ever have two pKs.
d. Are all chiral, except for glycine.
e. Occur in nature exclusively in the optically active D-form.
4. An enzyme in liver which is part of both the glycolytic and gluconeogenic pathways is:
a. glucose 6 phosphatase d. glucokinase
b. PEP carboykinase e. glyceraldehydes 3-phosphate dehydrogenase
c. Fructose 1,6-bisphosphatase
5. Fructose, 26-biphosphate:
a. is required for gluconeogenesis
b. synthesis is stimulated by insulin
c. is increased by cyclic AMP
d. inhibits phosphofructokinase
e. stimulates fructose, 1,6-bisphosphatase
7. Your patient is a 25 year old male who was admitted to the hospital in a state of starvation. He
had been homeless for several months and become depressed and had not eaten for 4 days.
You start him on a high carbohydrate diet as an attempt to spare protein and restore normal
blood sugar regulation. Which of the following enzymes is most likely to increase in concentration
in his liver?
a. Glucose 6-phosphatase d. Succinate dehydrogenase
b. Glucokinase e. Hexokinase
c. Pyruvate kinase
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10. Which enzyme would be impaired in a biotin deficiency.
a. fructose 1,6-bisphosphatase d. pyruvate carboxylase
b. pyruvate kinase e. malate dehydrogenase
c. PEP carboxykinase
11. Which of the following is the correct sequence of carriers in the electron transport chain?
a. NADH/CoQ reductase (R ) cytochrome c ® Co Q ® cytochrome reductase ® cytochrome
oxidase.
b. CoQ ® cytochrome reductase ® cytochrome oxidase ® cytochrome c ® NADH/CoQ
oxidase.
c. NADH/CoQ reductase ® CoQ (R)cytochrome c ® cytochrome oxidase ® cytochrome
reducatse.
d. NADH/CoQ reductase ® cytochrome reudcatse ® cytochrome c R) CoQ ® cytochrome
oxidase.
13. ATP is considered to be the universal source of energy for endergonic reactions but is not the
storage form of energy under physiological conditions. Why?
a. The actual free energy of hydrolysis of ATP is only -11 to -12 kcal.
b. ATP is not located in the cytoplasm where most endergonic reactions occur.
c. The half-life of ATP is too short.
d. The synthesis rate for ATP is not efficient.
14. The viability of red blood cells is critically dependent on glycolysis as a source of energy.
Glycolysis will cease if:
a. The concentration of cAMP is decreased.
b. Phosphofructokinase is activated.
c. Mitochondria are not present in the cell.
d. NADH is not oxidized
e. The concentration of AMP is increased.
17. Which of the following is present in the liver, but is absent in muscle?
a. pyruvate carboxylase d.. pyruvate dehydrogenase
b. glycogen synthetase e. glucose 6-phsophatase
c. lactate dehydrogenase
18. When are the concentrations of the conjugate acid and conjugate base equal?
a. When the pH equals the pK.
b. When the pH is 10x the pK.
c. When the pH is 0.1X the pK.
d. When the pH is 7.0
e. When the (H+) is equal to the (OH-)
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20. What is the value of D Go’ for the pathway A B CD?
a. Reaction D Go’
B <> A + 5 kcal/mol
C < == > + 5 kcal/mol
C > D -15 kcal/mol
b. +5
c. -10
d. -15
e. -20
23. The tricarboxylic acid cycle is initiated by the condensation of acetyl – CoA and
a. Malate d. oxaloaccetate
b. Citrate e. HCO3-
c. Pyruvate
24. Gluconcogenesis
a. is favored when insulin concentrations are high.
b. Occurs in the liver
c. Allows skeletal muscle to produce free glucose
d. Is stimulated by high levels of fructose 2,6-bisphosphate
e. Uses acetyl CoA as a substrate
25. Which of the following statements is TRUE? Enzyme catalysis of a chemical reaction.
a. increases the energy of the transition state.
b. Decreases the change in free energy of the reaction.
c. Increases the change in free energy of the reaction.
d. Decreases the change in the free energy of activation.
26. The activity of which enzyme would be expected to increase as the level of insulin increases?
a. Pyruvate dehydrogenase
b. Hexokinase
c. Glucose 6 –phosphatase
d. Fructose 1, 6-biphosphatase
e. PEP carboxykinase
27. Your patient has muscular weakness and degeneration of the optic nerve. Your tentative
diagnosis is Leber’s hereditary optic neuropathy. If this is the correct diagnosis, which of the
following pathways will contain the defect?
a. Tricarboxylic acid cycle
b. Electron transport
c. Glycolysis
d. Gluconeogenesis
e. Pyruvate dehydrogenase multienzyme complex
28. Which of the following statements about enzyme substrate complexes is TRUE?
a. At high substrate concentration the rate of the reaction is first order.
b. The enzyme concentration is higher than the substrate concentration under physiological
conditions.
c. Alteration in the conformation of the enzyme may occur when substrate binds.
d. Increase in enzyme concentration does not affect the rate of the reaction.
29. Which of the following is physiological mechanism for regulating the catalytic activity of enzymes?
a. Increasing the synthesis of the substrate.
b. Covalent modification of tryptophan residues.
c. Phosphorylation of histidine residues in the active site of the enzyme
d. Conformational alteration in the enzyme by binding regulatory proteins.
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30. The synthesis of phosphoenolpyruvate from pyruvate:
a. is the last step in glycolysis
b. is localized only in the cystosol
c. never occurs since the pyruvate kinase reaction is irreversible
d. consumes GTP
e. requires pyruvate kinase
32. You are the team physician for your local women’s soccer team. Some of your new recruits have
been disabled due to injury for the past four months and are about to start
a. Lactate dehydogenase
b. PEP carboxykinase
c. Transaminase
d. Hexokinase
e. Succinate dehydrogenase
36. A patient complained of painful cramps in her legs during exercise. Her patient history also
revealed that she experienced easy fatigability. Which of the following are consistent with these
observation?
a. The patient is diabetic
b. The patient is missing the enzyme glucose -6 –phosphatase
c. The patient may have a defective debranching enzyme.
d. The patient may have a defective muscle phosphorylase.
e. The patient had no liver glycogen.
37. When the arterial blood pH is 7.23, the plasma bicarbonate concentration is 10 meq/liter and the
PCO2 is 25 mm of Hg, (normal ranges: pH 7.35- 7.45; HC03-21-28-PC02 35-45) the acid-base
disturbance is:
a. Uncompensated respiratory acidosis
b. Uncompensated metabolic acidosis
c. Partially compensated metabolic acidosis
d. Compensated metabolic acidosis
e. Partially compensated respiratory acidosis
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e. HMG-CoA reductase is stimulated by insulin.
41. How does the cell maintain a higher level of K + than of Na+?
a. The Na + leaks out into the surrounding extracellular fluid.
b. K + is bound to sites inside the cell
c. The body contains less total Na+ than K+, so Na+ is found at a low concentration inside
the cell.
d. Na+ is actively transported out of the cell and K+ actively transported into the cell.
e. Much of the Na + outside the cell is bound to protein, so that no Na + gradient exists
across the cell membrane.
42. In the biosynthesis of purines the first purine derivative formed as:
a. Inosine c. guanosine monophosphate
b. inosine monophosphate d. adenosine monophosphate
e. xantosine monophosphate
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44. Which one of the following compounds is a COMMON intermediate in ketogenesis and
cholesterogenesis?
a. mevalonic acid d. HMG-CoA
b. acetoacetic acid e. malonyl-CoA
c. succinyl-CoA
47. The following are true statements about proteins in biological membranes EXCEPT:
a. Proteins in membranes can be attached either peripherally or penetrate, even span, the
lipid bilayer.
b. They are usually asymmetrically distributed on the different sides of the bilayer.
c. They are involved in transport processes through the lipid bilayer without changing sides
(flip-plop) from one side to the other.
d. They are immobile and fixed to the surrounding lipids.
e. Integral proteins usually contain a sequence of hydrophobic amino acids arranged as
alphahelix or beta-sheets.
48. Chronic alcohol intake will elevate the chylomicrons in blood. Which one of the following is a
normal constituent of chylomcirons
a. apoB-100 c. lipoprotein lipase
b. triglycerides d. free fatty acids
49. Which of the following enzymes would show the greatest increase in serum activity in viral
hepatitis?
a. creatine kinase d. 5’ nuleotidase
b. alkaline phosphatase e. acid phosphatase
c. alamine aminotransferase
50. Which of the phospholipids deficient in the lungs of patients with ARDS (acute respiratory
distress syndrome)?
a. Phosphatidylcholine d. cardiolipin
b. Phosphatidylinositol e. sphinagomyelin
c. phosphatidic acid
51. Increased excretion of urobilinogen in the urine is most commonly the result of
a. Hemolysis d. iron
b. erythropoetic porphyria e. bile duct obstruction
c. liver disease such as viral hepatitis
52. Which of the following statements about the pentose phosphate pathway is INCORRECT?
a. N5-methyl tetrahydrofolate (THF) monoglutamate
b. N5, 10-methylene THF
c. N10-formyl THF
d. N5-10-methenyl THF
e. dihydrofolate
53. The “methyl trap” hypothesis states that in the absence of vitamin B12, there is a block in the
further metabolism of
a. ADP d. ATP
b. Magnesium ions e. calcium ions
c. Inorganic phosphate
54. Actin is released from its interaction with myosin by the addition of:
a. ADP d. ATP
b. Magnesium ions e. calcium ions
c. Inorganic phosphate
55. As regards receptors coupled to Gs (stimulatory G protein) and cAMP, which of the following is
FALSE?
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a. Binding of a hormone to its receptor activates an enzyme which catalyzes the displaces
of GDP with GTP on the alpha Gs subunit.
b. The Gs alpha subunit dissociates from the beta/gamma subunits and activates adenylate
cyclase.
c. Adenylate cyclase catalyzes the conversion of ATP to CAMP.
d. cAMP actiats cAMP dependent protein kinase by binding to the regulatory subunits which
causes dissociation of the regulatory subunits from the cataylitic subunits.
e. The catalytic subunit cAMP dependent protein kinase phosphorylates proteins on serine.
57. For transport across the inner mitochondrial membrane, long chain fatty acids are attached to:
a. coenzyme A d. acyl transferase
b. acyl transacylase e. carnitine
c. phosphatidylethanolamine
59. A major contributing factor to cataract formation in diabetes may be the accumulation of sorbitol
in the lens. For that to occur, glucose has to interact with:
a. hexokinase and phosphoglucose isomerase
b. fructose dehydrogenase
c. aldose reductase
d. hexokinase and glucose 6 – phosphate dehydrogenase
e. glucose oxidase
60. The sphingolipidoses are a group of diseases involving defective breakdown of ceramides.
Which one of the following is characteristic for a ceramide?
a. an N-linked fatty acid residue.
b. A residue of glycerol
c. A phosphorylcholine residue
d. Three fatty acid residues
e. Contains a thiol
62. Primaquine sensitivity is a result of a defect in the pentose phosphate pathway. Which of the
following is TRUE for the pentose phosphate pathway:
a. It is absent in red blood cells.
b. Its regulated step is catalyzed by glucose-6-phosphate dehydrogenase
c. It channels all glucose carbons into the production of ribose
d. It can provide the cell with NADH needed in the synthesis of fatty acids.
e. One of its function is to maintain cellular glutathione in the oxidized state.
63. Most urinary ammonium ions are derived by the enzymatic hydrolysis of:
a. glutamine c. histamine
b. glutamic acid d. 6-amino purine derivatives
65. Which one of the following enzymes is involved in the hydrolysis of triglycerides found in
chylomicrons?
a. lipoprotein lipase
b. fatty acyl CoA transferase
c. hormone sensitive lipase
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d. pancreatic lipase
e. cholesterol ester transfer protein
66. A genetic disease characterized by accumulation of excess phenylalanine in the tissues, mental
retardation, and excretion of phenylalanine metabolites in the urine is caused by a deficiency in
a. phenylalanine transaminase d. homogentisic acid oxidase
b. tyrosine transaminase e. phenylalanine decarboxylase
c. phenylalanine hydroxylase
67. The creatine kinase isoenzyme which is characteristic of heart muscle is:
a. the MB isoenzyme c. the BB isoenzyme
b. the MM isoenzyme d. the CK
68. A patient with a deficiency in the enzyme, thioesterase, is unable to release a fully formed fatty
acid from fatty acid synthase. The name for that fatty acid is:
a. arachidonic acid d. palmitic acid
b. an omega 3 fatty acid e. stearic acid
c. octadecanoic acid
69. Which one of the following factors is NOT essential for the conversion of prothrombin to thrombin
in the clot cascade?
a. Ca ion d. Factor V
b. Factor X e. Factor XIII
c. Phospholipid
70. A DNA deletion occurs 1000 bp upstream of the src phtoooncogene, causing it to be activated.
The sequence deleted is probably a(n);
a. Promoter d. silencer
b. Operator e. initiator
c. Enhancer
71. Which of the following is NOT a possible consequence of a single nucleotide deletion in the
coding region of an exon?
a. Change in reading frame
b. Diminution of polyadenylation
c. Deletion of the 5’ splice site.
d. Nothing (i.e., no consequence)
e. Loss of the start codon.
72. Which of the following enyzymes are not used in cDNA cloning?
a. RNA polymerase d. Restriction endonuclease
b. DNA polymerase e. DNA ligase
c. Reverse transcriptase
75. Which of the following proteins can introduce positive supercoils into DNA?
a. Primosome d. Single strand-binding protein
b. DNA ligase e. XPF protein
c. Helicase
76. A mutation that allows read-through of a stop codon during translation is called a
______mutation.
a. Termination d. suppressor
b. Transversion e. silencer
c. amber
77. The gene for albumin is highly expressed in the liver, which of the following is LEAST likely about
the chromatin near the gene?
a. The albumin gene is in a region of euchromatin in liver
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b. The DNA is highly methylated at pCpG sequences in the region.
c. Histones are less abundant in the region
d. Histones are acetylated in the region.
79. The first three nucleotides of a palindromic restriction endonodcase recognition sequence are 5’-
AAG3”. What is the entire six basepair recognition sequence (both strands)?
a. 5’AAG-3’
3-TTC-5’
b. 5-AAGCTT-3’
3-TTCGAA-5’
c. TTCGAA-5’
5’TTCGAA-3’
d. 5’AAGCTT-3
3’-TTCCTT-5’
e. None of the above
80. You have determined that a strand of DNA is GC-rich. Its complementary strand is:
a. AT-rich
b. Pyrimidine-rich
c. Purine-rich
d. GC rich
e. Cannot determine from the information given.
81. You have incorporated a radioactive “label” into a PCR primer. After 30 cycles
a. only in the original target DNA molecules
b. in 50% of the new DNA molecules
c. interspersed between the parent and child strands of both new DNA molecules
d. in 1005 of the new DNA molecules
e. A and D
82. Which of the following statements is FALSE about eukaryotic chromosome structure?
a. Centromeres contain alphoid repeats.
b. Protein scaffold required for complete compaction
c. High telomerase activity has been associated with some cancers
d. Mitchondrial chromosomes sometimes contain short telomeres.
e. Mitchondrial genes sometimes use a different genetic code.
85. A chronic alcoholic is admitted to your service with a blood pH of 7.2. Which of the following
explains this finding?
a. Ethanol is an acid that produces acidosis when consumed in large quantities
b. Ethanol is converted into lactic acid that will lower blood pH.
c. Ethanol elevates the NADH concentration in the liver causing an increased conversion of
pyruvate to lactate.
d. Ethanol decreases the respiratory rate leading to a buildup of CO2 that is converted to
carbonic acid.
e. Ethanol blocks electron transport causing a buildup of NADH and lactate.
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c. Immune response to influenza virus
d. An autoimmune response
e. Successful vaccination against poliovirus
88. All of the following are likely to occur after eating a large amount of carbohydrate EXCEPT
a. The synthesis of triacylgylcerol in the liver.
b. The synthesis of glycogen in the liver
c. Increased pentose phosphate pathway activity in liver
d. Release of glycerol from adipose tissue
e. The synthesis and release of VLDL from the liver
94. Your patient has a genetic defect that blocks the synthesis of androstenedione. Which of the
following will also be reduced by this defect?
a. Progesterone d. cortisol
b. Testosterone e. aldosterone
c. Pregnenolone
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a. glucose is both converted to glycogen and stored in skeletal muscle.
b. Adipose tissue converts triglycerides to glucose
c. Amino acids are transaminated to form ketone bodies.
d. Glycogen stores in liver are depleted
e. Glycogen phosphorylase is in the active form.
99. All of the statements below about T-cells are TRUE, EXCEPT which one?
a. The major histocompatibility complex class 1 protein is recognized by the CD8 (or T8)
protein of the cytotoxic T-cell
b. The major histocompatibility complex class 1 protein is recognized by the CD8 (or T8)
protein of the cytotoxic T-cell)
c. The T-cell antigen receptor is a heterodimeric protein with variable region domains and
constant region domains.
d. The major histocompatibility complex Class II protein is recognized by the CD4 (or T4)
protein of the helper T-cell.
e. The CD3 complex of the T-cell carries and displays the antigen fragment.
100. Twenty four hours after a meal, the primary source of glucose carbons for the brain is:
a. dietary glucose
b. muscle glycogen
c. glycerol from adipose tissue
d. fatty acids
e. skeletal muscle protein.
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