5/11/2020 PhysioEx Exercise 8 Activity 2

PhysioEx Lab Report

Exercise 8: Chemical and Physical Processes of Digestion
Activity 2: Exploring Amylase Substrate Specificity
Name: Nicole de León
Date: 5 November 2020
Session ID: session-49cb1852-9fb2-1abf-84de-0690fec13075

Pre-lab Quiz Results

You scored 100% by answering 5 out of 5 questions correctly.

1 Which of the following is true of enzymes and substrates?

You correctly answered: Enzymes are specific about the substrates they can act upon.

2 Which of the following is/are reducing sugars?

You correctly answered: both glucose and maltose .

3 Cellulose and starch are both

You correctly answered: polymers of glucose and polysaccharides.

4 Proteins and peptides are formed by joining amino acids with a special type of covalent
bond called a peptide bond. Which of the following enzymes do you think would be specific
for a peptide bond?

You correctly answered: peptidase.

5 The Benedict's assay tests for the presence of

You correctly answered: reducing sugar.

Experiment Results
Predict Question
1 Predict Question: Do you think test tube 3 (amylase, cellulose, pH 7.0 buffer) will show a
positive Benedict's test?

Your answer: no.

Stop & Think Questions

1 You included tube 2 (amylase, glucose, pH 7.0 buffer) to

You correctly answered: see what a positive Benedict's test should look like. i
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5/11/2020 PhysioEx Exercise 8 Activity 2

2 Which tube is included to detect contaminating amylase in the buffer or in cellulose?

You correctly answered: None of the above.

3 What is the usual substrate for the pancreatic enzyme peptidase?

You correctly answered: peptides.

Experiment Data
Tube No. Reagent 1 Reagent 2 Reagent 3 Time Temp (°C) IKI Benedict's
(min)
pH 7.0
1 Amylase Starch 60 37 - ++
Buffer
pH 7.0
2 Amylase Glucose 60 37 - ++
Buffer
pH 7.0
3 Amylase Cellulose 60 37 - -
Buffer
pH 7.0 Deionized
4 Cellulose 60 37 - -
Buffer Water
pH 7.0
5 Peptidase Starch 60 37 + -
Buffer
pH 7.0
6 Bacteria Cellulose 60 37 - ++
Buffer

Post-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 The substrate(s) for amylase is/are

You correctly answered: starch.

2 The results of tube 5 (peptidase, starch, pH 7.0 buffer) demonstrated that

You correctly answered: peptidase does not digest starch.

3 Why was the cellulose in tube 6 (bacteria, cellulose, pH 7.0 buffer) hydrolyzed to glucose?

You correctly answered: The bacterial suspension contained the enzyme cellulase, which
digested the cellulose.

4 Which tube allows you to conclude that the buffer was not contaminated with amylase?

You correctly answered: tube 5 (peptidase, starch, pH 7.0 buffer).

Review Sheet Results

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5/11/2020 PhysioEx Exercise 8 Activity 2

1 Describe why the results in tube 1 (amylase, starch, pH 7.0 buffer) and tube 2 (amylase,
glucose, pH 7.0 buffer) are the same.

Your answer:

En el tubo número 1 y número 2 salieron los mismos resultados debido a que en el tubo
número 1 la amilasa hidroliza el almidón convirtiéndolo en glucosa y en el tubo número 2 se
encuentra la glucosa tal cual, por lo que en ambos se encuentra un azúcar reductor
(glucosa).

2 Describe the result in tube 3 (amylase, cellulose, pH 7.0 buffer). How well did the results
compare with your prediction?

Your answer:

El resultado fue tal cual predije debido a que los resultados del tubo 3 muestran que la
amilasa salival no digiera la celulosa lo cual se demostró en la prueba Benedict dando
negativo.

3 Describe the usual substrate for peptidase.

Your answer:

El sustrato de la peptidasa es BAPNA. BAPNA es utilizado como sustrato para evaluar la

actividad de la pepsina. BAPNA es un péptido sintético que deja un producto de color
amarillo cuando hidroliza. La solución BAPNA se convierte en amarillo en presencia de una
peptidasa activa, al igual que la pepsina.

4 Explain how bacteria can aid in digestion.

Your answer:

La bacteria ayuda en la digestión debido a que colonizan el colon, sintetizan las vitaminas
del complejo B y algunas del complejo K utilizadas para producir varias proteínas,
metabolizan algunas moléculas derivadas del huésped, entre otras.

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