12/1/21, 10:45 AM PhysioEx Exercise 8 Activity 4

PhysioEx Lab Report

Exercise 8: Chemical and Physical Processes of Digestion

Activity 4: Assessing Lipase Digestion of Fat

Name: DK 15

Date: 1 December 2021

Session ID: session-3361c230-2549-fc39-b787-a63133a9b6bb

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 Which of the following is/are true of bile?

You correctly answered: It works by a physical process.

2 The substrate used in this simulation is

You correctly answered: vegetable oil.

3 When fatty acids are liberated by lipase, the pH

You correctly answered: decreases.

4 One of the products of the chemical digestion of lipids is

You correctly answered: fatty acids.

Experiment Results
Predict Question
1 Predict Question: Which tube do you think will have the highest lipase activity?

Your answer: tube 1 (lipase, vegetable oil, bile salts, pH 7.0 buffer).

Stop & Think Questions

1 What does the pH measure?

You correctly answered: both lipase activity and fatty acid release.

2 Why is it difficult to detect whether lipase is active in tube 5 (lipase, vegetable oil, bile
salts, pH 2.0 buffer)?

You correctly answered: The pH is already very low, so a decrease in pH might be difficult
to detect.

Experiment Data
Tube Reagent Reagent Reagent Reagent Time Temp pH
No 1 2 3 4 (min) (°C)
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12/1/21, 10:45 AM PhysioEx Exercise 8 Activity 4

Vegetable pH 7.0
1 Lipase Bile salts 60 37 6.21
Oil Buffer
Vegetable Deionized pH 7.0
2 Lipase 60 37 7.00
Oil Water Buffer
Deionized pH 9.0
3 Lipase Bile salts 60 37 9.00
Water Buffer
Deionized Vegetable pH 7.0
4 Bile salts 60 37 7.00
Water Oil Buffer
Vegetable pH 2.0
5 Lipase Bile salts 60 37 2.00
Oil Buffer
Vegetable pH 9.0
6 Lipase Bile salts 60 37 9.00
Oil Buffer

Post-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 What is the product of lipase hydrolysis?

You correctly answered: fatty acids.

2 From your results, which pH is ideal for pancreatic lipase digestion?

You correctly answered: pH 7.0.

3 Which tube confirms that there is no lipase in bile salts or vegetable oil?

You correctly answered: tube 4 (deionized water, vegetable oil, bile salts, pH 7.0 buffer).

4 Where in the body does pancreatic lipase hydrolyze triglycerides?

You correctly answered: small intestine.

Review Sheet Results

1 Explain why you can't fully test the lipase activity in tube 5 (lipase, vegetable oil, bile salts,
pH 2.0 buffer).

Your answer:

karena pH sudah terlalu rendah sejak awal, sehingga penurunan sulit untuk dideteksi.

2 Which tube had the highest lipase activity? How well did the results compare with your
prediction? Discuss possible reasons why it may or may not have matched.

Your answer:

tube 1. lipase, vegetable oil, bile salts, pH 7.0 buffer. karena pH 7 merupakan pH yang
ideal dalam pencernaan oleh lipase. ditunjukkan oleh penurunan pH yang terlihat
sebelum dan sesudah reaksi.
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12/1/21, 10:45 AM PhysioEx Exercise 8 Activity 4

3 Explain why pancreatic lipase would be active in both the mouth and the pancreas.

Your answer:

karena mulut memiliki pH sekitar 7 sehingga lipase juga dapat aktif di sana.

4 Describe the process of bile emulsification of lipids and how it improves lipase activity.

Your answer:

garam empedu disekresikan ke dalam usus halus untuk mengemulsi lemak secara fisik.
garam empedu bersifat seperti detergent, memisahkan gumpalan lipid dan
meningkatkan area permukaan yang dapat diakses oleh enzim lipase sehingga
meningkatkan aktivitas lipase itu sendiri.

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