18/10/21 14:10 PhysioEx Exercise 8 Activity 3

PhysioEx Lab Report

Exercise 8: Chemical and Physical Processes of Digestion
Activity 3: Assessing Pepsin Digestion of Protein
Name: darly lux
Date: 18 October 2021
Session ID: session-3750ed07-8131-204b-6cdb-d7f20d3b1324

Pre-lab Quiz Results

You scored 100% by answering 5 out of 5 questions correctly.

1 Where in the body does protein digestion begin?

You correctly answered: the stomach.

2 The substrate for pepsin is

You correctly answered: protein and peptides.

3 In this activity the substrate you will be using to detect protein digestion is

You correctly answered: BAPNA.

4 Negative results with the negative controls

You correctly answered: are expected and validate the experiment.

5 A spectrophometer measures

You correctly answered: optical density.

Experiment Results
Predict Question
1 Predict Question: At which pH do you think pepsin will have the highest activity?

Your answer: pH 9.0.

Stop & Think Questions

1 Which of the following tubes are negative control tubes?

You correctly answered: tubes 3 (pepsin, deionized water, pH 2.0 buffer) and 4
(deionized water, BAPNA, pH 2.0 buffer).

2 An optical density greater than zero indicates that

You did not answer this question.

Correct answer: BAPNA digestion has occurred and pepsin is active.

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18/10/21 14:10 PhysioEx Exercise 8 Activity 3

Experiment Data
Tube Reagent 1 Reagent 2 Reagent 3 Boiled Time Temp Optical
No. (min) (°C) Density
pH 2.0
1 Pepsin BAPNA - 60 37 0.40
Buffer
pH 2.0
2 Pepsin BAPNA - 60 37 0.40
Buffer
Deionized pH 2.0
3 Pepsin - 60 37 0.00
Water Buffer
Deionized pH 2.0
4 BAPNA - 60 37 0.00
Water Buffer
pH 7.0
5 Pepsin BAPNA - 60 37 0.03
Buffer
pH 9.0
6 Pepsin BAPNA - 60 37 0.00
Buffer

Post-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 Pepsin would be most active

You correctly answered: in the stomach.

2 Which two tubes validated the results of the experiment?

You correctly answered: tubes 3 (pepsin, deionized water, pH 2.0 buffer) and 4
(deionized water, BAPNA, pH 2.0 buffer).

3 With more enzyme activity the optical density

You correctly answered: increased.

4 If pepsin were digesting an actual protein substrate, the product would be

You correctly answered: peptides and amino acids.

Review Sheet Results

1 Describe the effect that boiling had on pepsin and how you could tell that it had that
effect.

Your answer:

Hervir inactivo la pepsina, ya que no hubo actividad en el tubo 1 pero fue activo en el
tubo 2.

2 Was your prediction correct about the optimal pH for pepsin activity? Discuss the
physiological correlation behind your results.

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Your answer:

Fue acertado ya que la pepsina es la mas activa.

3 What do you think would happen if you reduce the incubation time to 30 minutes for
tube 5 (pepsin, BAPNA, pH 7.0 buffer)?

Your answer:

Cuando hay mas actividad enzimática aumenta la densidad, si se realizara el

experimento un resultado probable seria que la densidad óptica disminuiría o seria 0.

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