2/11/22, 4:23 PM PhysioEx Exercise 8 Activity 2

PhysioEx Lab Report

Exercise 8: Chemical and Physical Processes of Digestion
Activity 2: Exploring Amylase Substrate Specificity
Name: Maria Karla M. Balamad
Date: 11 February 2022
Session ID: session-233c3343-9599-4417-bb7b-b2d40a34ab8a

Pre-lab Quiz Results

You scored 100% by answering 5 out of 5 questions correctly.

1 Which of the following is true of enzymes and substrates?

You correctly answered: Enzymes are specific about the substrates they can act upon.

2 Which of the following is/are reducing sugars?

You correctly answered: both glucose and maltose .

3 Cellulose and starch are both

You correctly answered: polymers of glucose and polysaccharides.

4 Proteins and peptides are formed by joining amino acids with a special type of covalent
bond called a peptide bond. Which of the following enzymes do you think would be specific
for a peptide bond?

You correctly answered: peptidase.

5 The Benedict's assay tests for the presence of

You correctly answered: reducing sugar.

Experiment Results
Predict Question
1 Predict Question: Do you think test tube 3 (amylase, cellulose, pH 7.0 buffer) will show a
positive Benedict's test?

Your answer: yes.

Stop & Think Questions

1 You included tube 2 (amylase, glucose, pH 7.0 buffer) to

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2/11/22, 4:23 PM PhysioEx Exercise 8 Activity 2

Your answer: make sure the enzyme amylase is working.

Correct answer: see what a positive Benedict's test should look like.

2 Which tube is included to detect contaminating amylase in the buffer or in cellulose?

You correctly answered: None of the above.

3 What is the usual substrate for the pancreatic enzyme peptidase?

You correctly answered: peptides.

Experiment Data
Tube No. Reagent 1 Reagent 2 Reagent 3 Time Temp (°C) IKI Benedict's
(min)
pH 7.0
1 Amylase Starch 60 37 - ++
Buffer
pH 7.0
2 Amylase Glucose 60 37 - ++
Buffer
pH 7.0
3 Amylase Cellulose 60 37 - -
Buffer
pH 7.0 Deionized
4 Cellulose 60 37 - -
Buffer Water
pH 7.0
5 Peptidase Starch 60 37 + -
Buffer
pH 7.0
6 Bacteria Cellulose 60 37 - ++
Buffer

Post-lab Quiz Results

You scored 75% by answering 3 out of 4 questions correctly.

1 The substrate(s) for amylase is/are

You correctly answered: starch.

2 The results of tube 5 (peptidase, starch, pH 7.0 buffer) demonstrated that

You correctly answered: peptidase does not digest starch.

3 Why was the cellulose in tube 6 (bacteria, cellulose, pH 7.0 buffer) hydrolyzed to glucose?

Your answer: The bacterial suspension contained the enzyme amylase, which digested the
cellulose.

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2/11/22, 4:23 PM PhysioEx Exercise 8 Activity 2

Correct answer: The bacterial suspension contained the enzyme cellulase, which digested
the cellulose.

4 Which tube allows you to conclude that the buffer was not contaminated with amylase?

You correctly answered: tube 5 (peptidase, starch, pH 7.0 buffer).

Review Sheet Results

1 Describe why the results in tube 1 (amylase, starch, pH 7.0 buffer) and tube 2 (amylase,
glucose, pH 7.0 buffer) are the same.

Your answer:

The results showed that the tube 1 and tube 2 were the same because the amylase in tube 1
is hydrolyzing the starch to the glucose. In tube 2 the glucose is already in its hydrolyzed
form.

2 Describe the result in tube 3 (amylase, cellulose, pH 7.0 buffer). How well did the results
compare with your prediction?

Your answer:

I originally predicted yes, but I found that test tube #3 did not show Benedict's test after
experimenting. This is because the amylase did not digest the cellulose in the solution.

3 Describe the usual substrate for peptidase.

Your answer:

The usual substrates for peptidase are peptides and proteins.

4 Explain how bacteria can aid in digestion.

Your answer:

Bacteria aids in digestion by breaking down cellulose because the body cannot do it
naturally without cellulase.

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