Name: 201983019_Nilam Kakiay_4

Exercise 8: Chemical and Physical Processes of Digestion: Activity 4: Assessing Lipase Digestion of Fat Lab Report

Pre-lab Quiz Results

You scored 75% by answering 3 out of 4 questions correctly.

1. Which of the following is/are true of bile?

Your answer : a. It works by a chemical process.
Correct answer: b. It works by a physical process.

2. The substrate used in this simulation is

You correctly answered: c. vegetable oil.

3. When fatty acids are liberated by lipase, the pH

You correctly answered: b. decreases.

4. One of the products of the chemical digestion of lipids is

You correctly answered: d. fatty acids.

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Experiment Results
Predict Question:
Predict Question: Which tube do you think will have the highest lipase activity?
Your answer : b. tube 2

Stop & Think Questions:

What does the pH measure?
You did not answer this question.
Correct answer: d. both lipase activity and fatty acid release

Why is it difficult to detect whether lipase is active in tube 5?

You did not answer this question.
Correct answer: d. The pH is already very low, so a decrease in pH might be difficult to detect.

Experiment Data:

Tube No. Reagent 1 Reagent 2 Reagent 3 Reagent 4 Time Temp. pH

1 Lipase Vegetable Oil Bile Salts pH 7.0 Buffer 60 37 6.21
2 Lipase Vegetable Oil Deionized pH 7.0 Buffer 60 37 7.00
Water
3 Lipase Deionized Water Bile Salts pH 9.0 Buffer 60 37 9.00
4 Deionized Water Vegetable Oil Bile Salts pH 7.0 Buffer 60 37 7.00
5 Lipase Vegetable Oil Bile Salts pH 2.0 Buffer 60 37 2.00
6 Lipase Vegetable Oil Bile Salts pH 9.0 Buffer 60 37 9.00

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Post-lab Quiz Results
You scored 25% by answering 1 out of 4 questions correctly.

1. What is the product of lipase hydrolysis?

Your answer: b. triglycerides
Correct answer: d. fatty acids

2. From your results, which pH is ideal for pancreatic lipase digestion?

You correctly answered: b. pH 7.0

3. Which tube confirms that there is no lipase in bile salts or vegetable oil?
Your answer: b. tube 3
Correct answer: c. tube 4

4. From your results, where (in theory) would pancreatic lipase be active?
Your answer: b. stomach
Correct answer: d. mouth and pancreas

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Review Sheet Results
1. Explain why you can't fully test the lipase activity in tube 5.
You did not answer this question.

2. Which tube had the highest lipase activity? How well did the results compare with your prediction? Discuss possible
reasons why it may or may not have matched.
You did not answer this question.

3. Explain why pancreatic lipase would be active in both the mouth and the pancreas.
You did not answer this question.

4. Describe the process of bile emulsification of lipids and how it improves lipase activity.
You did not answer this question.

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