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  • Using Lineweaver Burk Equation

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    Diana Bunagan
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    The document summarizes using the LineWeaver Burk equation to determine the Michaelis-Menten constant (Km) and maximum reaction velocity (Vm) for an enzyme reaction. It shows that Km is approximately 0.76 and Vm is approximately 3 based on the given values. It then uses another equation to determine that the maximum velocity (Vmax) is 300 and the Michaelis constant (Km) is approximately 2/5. The closest matching values for Km and Vm according to the choices given are 1.89 and 0.55 respectively.

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    BIOCHEM ASSIGN

    Original Title

    bIOCHEM

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    The document summarizes using the LineWeaver Burk equation to determine the Michaelis-Menten constant (Km) and maximum reaction velocity (Vm) for an enzyme reaction. It shows that Km is approximately 0.76 and Vm is approximately 3 based on the given values. It then uses another equation to determine that the maximum velocity (Vmax) is 300 and the Michaelis constant (Km) is approximately 2/5. The closest matching values for Km and Vm according to the choices given are 1.89 and 0.55 respectively.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    4 views2 pages

    Using Lineweaver Burk Equation

    Uploaded by

    Diana Bunagan
    The document summarizes using the LineWeaver Burk equation to determine the Michaelis-Menten constant (Km) and maximum reaction velocity (Vm) for an enzyme reaction. It shows that Km is approximately 0.76 and Vm is approximately 3 based on the given values. It then uses another equation to determine that the maximum velocity (Vmax) is 300 and the Michaelis constant (Km) is approximately 2/5. The closest matching values for Km and Vm according to the choices given are 1.89 and 0.55 respectively.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 2

    1.

    2. Using LineWeaver Burk Equation

    Km/Vm = .2604

    Vm = 1/.3407 = 2.935134 approximately 3

    Km= vm*(km/vm) =.2604*2.9351 =.764 approximately 8

    K2= vmax/[E] = 3E-06/E-08= 300

    Vmax [ S ]
    3. 0.12 V = where km∧S are equal
    km+S
    Vmax [ S ] 6
    0.12 V = cancelboth s will give you value of alsoequal ¿ 2/5
    2S 25

    4. Using Eadie-Hofstee
    Km=1.2114

    Vm=.4499

    Nearest value in the choices is Km=1.89, Vm=.55

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