Professional Documents
Culture Documents
ABSTRACT
Chaieb, I. 2010. Saponins as insecticides: a review. Tunisian Journal of Plant Protection 5: 39-
50.
Saponins are heterosides (substances containing in their structure one or more sugar molecule) of plant
origin. This type of molecules has an interesting pesticide potential and this review constitutes an
inventory of principal researches realized in this direction. In the first part of this review, saponins are
defined and their different structural families are presented. The biological significance and principal
sources of saponins were also outlined. The second part of this review focused on insecticidal activities
of saponins. In fact, these substances are known by their toxicity to harmful insects (anti-feeding,
disturbance of the moult, growth regulation, mortality...); the insecticidal activity of saponins is due to
their interaction with cholesterol, causing a disturbance of the synthesis of ecdysteroids. These
substances are also protease inhibitors or cytotoxic to certain insects. The third part of the review gave
an idea on the limits which can slow down the use of saponins as insecticides: saponins have a strong
toxicity to mammals because of their cytotoxic and haemolytic activities. The second constraint is the
loss of molecule activity due to degradation of sugars associated with the aglycone. The hydrophilic
nature of saponins limits their penetration through the lipophilic insect cuticle. The structural
complexity of saponins limits the exact identification and synthesis of active molecules.
O
18
OH
22
O 24
19
1 16
15
CH2OH Aglycone
OO 3 5
6
OH
1'
O OO
CH2OH
CH3 1'' O
O Sugar chain
OH
1''' OH OH
HO
HO O
OH
CH3 1''''
OH OH
Fig. 1. Example of steroidic saponin with 4 sugar chains: Parquisoside 1 extracted from Cestrum parqui (7)
R
R= Hopane
R= Dammarane
Sugars
R=
Ursane
Triterpenic aglycone
R O
R= Spirostane
O
N
R= Glycoalcaloid
Sugars R= Furostane
Steroidic aglycone
Spodoptera littoralis Cestrum parqui saponins Impossibility to get free from the old cuticle (16)
during the molting process
Shistocerca gregaria Cestrum parqui saponins Ecdysial disturbances (9)
Entomotoxicity. The crude saponins and the blossoming eggs rate are observed
extracted from Cestrum parqui injected to in the same insect species (1). The
the L5 Schistocerca gregaria larva saponins extracted from the leaves and
increase insect mortality (9). In the same the roots of the alfalfa are toxic for
way, the spray of tomato leaves by 0.1 to Leptinotarsa decemlineata larvae (49).
0.2% of an aqueous solution of alfalfa The addition of aginoside 1
saponins reduces the number of (steroidic saponin) to the artificial diet of
Tetranychus urticae mite and Pharodon Acrolepiosis assectella larvae with an
sp. aphids by 85 and 90%, respectively. amount of 0.9 mg/g, causes 56% of
Saponins of alfalfa can also cause mortality (29). The commercial saponins
mortalities on eggs of T. urticae (37). extracted from Quillaja saponaria have a
The introduction of alfalfa saponins larvicidal activity against the mosquitos
into the food of Ostrinia nubilalis cause larvae of two species Aedes aegypti and
larval mortalities reaching 100% for the Culex pipiens; 100% of mortality is
L2 larval stages. Mortalities were also obtained by using amounts of 1000 mg/l
recorded for the nymphal stage; during 5 days (40).
moreover, only 60% of the treated Crude saponins of Cestrum parqui
chrysalis emerge (36). Treated by 100 showed a variable toxicity on various
ppm saponin of alfalfa leaves, Spodoptera tested insects (Schistocera gregaria, S.
littoralis shows a cumulative mortality of littoralis and Tribolium confusum) but the
90% at the larval and the nymphal stages most significant toxicity was observed on
(1). Various forms of chronic toxicity as a the larvae of the mosquito Culex pipiens
reduction in the fertility of the females (14).
Tunisian Journal of Plant Protection 43 Vol. 5, No. 1, 2010
Forming insoluble complexes with biosynthesis of the ecdysone and explain
saponins, cholesterol is not absorbed any the disturbance of moulting process often
more by the digestive system of various observed following ingestion of Cestrum
animal species. The mechanism of parqui leaves (9) or by the incorporation
formation of the cholesterol/saponin of extracts in the insect diet (15).
complexes is still unknown. Certain Various natural or synthesized
authors suggest a chemical reaction insecticidal substances affecting the
between the saponic aglycone and the biosynthesis or the mechanisms of action
lipophylic sites of cholesterol (51); Mitra of ecdysone, have a disturbing effects on
and Dungan (35) show that there is a insect growth and moulting (5, 6). In fact,
formation of micelle or spheres structures saponins are substances often cited in the
between cholesterol and saponin literature as provoking difficulties of
molecules. exuviations and malformations of various
The hypocholesterolemic activity of insect species. Some of these works
saponins was largely studied in many evoke the possibility of interaction of
mammals (20, 34). Is such saponins with cholesterol but no
cholesterol/saponin interaction possible in demonstration was made until now.
insects? Theoretically yes, since insects, Some experiments (Table 2) showed
while being unable to synthesize an Insect Growth Regulator activity of
cholesterol, they use this substance in the Cestrum parqui saponins. Indeed, insects
biosynthesis of the ecdysone (moulting consuming saponins supplemented with
hormone) and various other ecdysteroids. cholesterol support better the toxic effect
This hypo- hypocholesterolemic of saponins; this fact is in favor of an
mechanism, similar to that observed in antagonistic effect of cholesterol and
the mammals following the action of consolidates our assumption concerning
saponins, could interfere with the the mode of action of saponins (17).
Table 2. Effects of cholesterol addition in the diet of some insects treated with different saponins
Insect species Saponins used Effects of cholesterol addition Reference
Acrolepiopsis assectella Aginosid Reduce the larval mortality from 56% (29)
to 22% and moulting failures from 19
to 8%
Acrolepiopsis assectella Digitonin Reduction in the death rate from 62 to (5)
27%
Acrolepiopsis assectella Digitonin Removes completely the toxicity (6)
Tribolium confusum Cestrum parqui saponins Reduction of larval mortality from 95 (17)
to 45%
Tenebrio molitor Alfalfa saponins Elimination of the saponin toxicity (43)
Tribolium castaneum Solmargine, Solasonine, Tomatine Increase the viability of treated larvae (55)
Several authors (29, 43, 55) suppose solvents (14), whereas certain works
a possible interaction saponin/cholesterol reported formation of a precipitate with
causing cholesterimic deficit in insect, similar reactions (26, 51).
disturbing the ecdysone synthesis. This The mechanisms of interaction of
complexation can occur in food, saponins with cholesterol are still
hemolymph, or inside the insect cells. unknown and according to certain
Studies trying to react in vitro cholesterol authors, there is no formation of an
with saponin remained unfruitful intermediate compound but a spherical
although the use of various methods and structure, intercalation between saponin
Tunisian Journal of Plant Protection 44 Vol. 5, No. 1, 2010
molecule and cholesterol, called micelle tract of Schistocerca. In addition, the cells
(35) or tubular structures (32) may be of the fat body appear darker due to the
involved. Consequently, saponins do not loss of their contents probably caused by
block cholesterol or other phytosterols in the modification of their membrane
the food, but this reaction could take permeability, and even with the
place later inside insect body where other disorganization of their molecular
conditions are satisfied (pH, enzymatic architecture (19).
arsenal). In addition to the moulting
Other scientific attempts to disturbance and the cytotoxic activity,
proportionate cholesterol in insects certain authors evoke an inhibitory
consuming saponins did not lead to activity of the digestive proteases of
reliable results because undoubtedly of saponins involved in the entomo-toxicity
methodologies used which would be recorded (9). Another work concerning
unsuited to very low circulating the effect of food treated by Cestrum
cholesterol rates. Cholesterol is not in parqui leaves on S. littoralis larvae shows
majority in the phytophagous insect food a deficit in the digestion of proteins and a
because plants contain other types of decrease of the protein rate in the
sterols as sitosterol and sigmasterol. It is hemolymph and the cuticle (16).
possible that this interference between
saponin and cholesterol would take place Limits of the use of saponins in
inside insect cells (17). Some authors phytoprotection.
suppose the possibility of interaction of Stability problems. Saponins are
saponin with ecdysteroid receptors (22, relatively big size molecules which
23). contain sugars whose degradation is
With the injection of crude saponins easier under certain conditions (pH
of Cestrum to S. gregaria locust, some slightly acid or basic, presence of
necrotic symptoms appear at the injection hydrolysis enzymes...). This degradation
site. In the same way, a forced ingestion leads to the loss of activity which
of crude saponins has, as a consequence, enormously depends on the water-soluble
a softening of the consistency of the sugar chains. The modification of the
digestive tract of S. gregaria adults. A structure of Cestrum parqui saponins (14)
pickling of the fat body of Spodoptera by the acetylation of sugars hydroxyls or
littoralis in saponins increases its tanning the separation of the aglycone by
(14). hydrolysis led to a loss of the insecticidal
Histological studies revealed activity of the molecule, which confirms
structural modifications at the fat body of results obtained by various authors (4, 9,
S. littoralis as well as on the foregut and 30, 32, 51).
the gastric caeca of S. gregaria. These Barbouche (9) already reported that
modifications were due to the cytotoxicity sapogenins of Cestrum parqui are less
effect of Cestrum parqui saponins (19). active than saponins; this demonstrates
Similar effects are obtained by treatment the loss of saponin’s activity following
of Culex pipiens mosquito larvae by their hydrolysis. Indeed, it has been
Cestrum parqui saponins (18). shown that the aglycone obtained was
The microscopic observations of inactive by grafting of these crystals in S.
treated insect tissue cuts show smaller gregaria, just like acetylated saponins. It
size cells than the control at the fat body seems that the various structural
of Spodoptora as well as at the digestive modifications are involved in the
Tunisian Journal of Plant Protection 45 Vol. 5, No. 1, 2010
hydrophily loss; the molecule needs the Cestrum parqui is interesting in
sugar chain for its solubility in the experiments of injection and forced
hemolymph and for its activity (14). ingestion. Death, in these cases, is
Moreover, various authors report the observed after a few hours. The problem
loss of the biological activity of saponins is that these experimental methods are
by structural modifications. Indeed, practically not applicable. It is necessary
Keukens et al. (32) showed that a to develop simpler and more effective
reduction of the chain of α-tomatine or of techniques. Treatments by topic
α-choacine increased the total loss of application do not give the anticipated
activity due to the membrane rupture. In results because of the impermeability of
the same way, a study of the the cuticle to saponins. Some researches
digitonine/cholesterol interaction shows tried to associate saponins with abrasive
that analogues of digitonine could be insecticides (diatomous earth) which can
associated with cholesterol. Various cause wounds on the cuticle; this
degrees of glycosylation of the digitonine association remains also unfruitful (14).
are used: two, four or five sugars are
associated to the aglycone, the results Synthesis difficulty. Saponins are
show that this complexation increases molecules characterized by a heavy
when the number of associated sugars molecular weight and an important
increases (51). structure complexity; this reduces their
Hu et al. (30) then Armah et al. (4) chance to be used like model to
confirm these results by using similar synthesize insecticidal molecules. Most
saponins having the same triterpenic works undertaking the synthesis of these
aglycone and by showing successively products do it only partially (28).
that the nature of sugar influences little on
the molecule activity, but that, on the Toxicity. Saponins have a cytotoxic
other hand, the hydrolysis of one, two or (27) haemolytic (52) effects and are able
three sugars increases the total or partial of inhibiting the proteases activities (56);
loss of activity. this represents a constraint if we attempt
to apply these substances as agricultural
Antifeedancy. There is another products. These saponins are, in fact,
problem which makes delicate the rather as toxic for pests as for human.
practical application of saponins as
insecticide; it is the repulsive or Conclusion.
antifeeding activity of saponins to several Secondary substances in plants are
pest insects. Indeed, it was noticed that known for a long time for their medicinal
saponins decrease very appreciably the and pharmacological properties. These
quantity of food consumed; this substances are necessary for the plant to
phenomenon seems to be a defense evolve in a hostile environment. The plant
reaction of the animal against these toxic can indeed use its secondary metabolites
substances; this have as consequence the to be protected against several pest
reduction in the quantity of active animals and pathogenic microbes.
molecules introduced by ingestion and Saponins present one of these
then reduction of the activity (14). substances of large action spectrum
broad, because of their toxicity to various
Problems of application. The insects. The mode of action of saponins
insecticidal activity of saponins of seems in relation to the property of these
Tunisian Journal of Plant Protection 46 Vol. 5, No. 1, 2010
molecules to be interacted either with Saponins present an excellent model
structural cholesterol (membrane) or with of study of natural substances with
metabolic cholesterol (food). insecticidal effect due to their large
The practical application of this type spectrum of action and to the multitude of
of substances remains difficult because of their physiological effects. It is, however,
easy degradation of these substances, the early to recommend application of
impossibility of acting by contact, the saponins as insecticides. Thorough
difficulties of their synthesis and their studies of their modes of action and
toxicity to mammals. application should be done firstly.
___________________________________________________________________________
RESUME
Chaieb I. 2010. Les saponines comme insecticides: revue de synthèse. Tunisian Journal of Plant
Protection 5: 39-50.
Les saponines sont des hétérosides (molécules ayant au moins un sucre dans leur structure) d’origine
végétale. Ce type de molécules présente un potentiel insecticide faisant l’objet de cette synthèse. Dans
la première partie de notre étude, nous avons essayé de les définir et de présenter leurs différentes
familles structurales. Un aperçu sur la signification biologique et les principales sources de saponines
est donné. La deuxième partie de cette synthèse s’intéresse aux principaux travaux réalisés sur les
différentes activités insecticides. Ces substances occasionnent plusieurs formes de toxicité à l’encontre
des insectes nuisibles (anti-appétence, perturbation de la mue, régulation de la croissance, mortalité…);
l’activité insecticide des saponines proviendrait de leur interaction avec le cholestérol causant une
perturbation de la synthèse des ecdysteroïdes. Ces substances possèdent également des propriétés
inhibitrices de protéases et cytotoxiques. Dans la troisième partie de ce travail, nous avons donné une
idée sur les contraintes qui peuvent freiner l’utilisation des saponines comme insecticides: les
saponines présentent, en effet, une forte toxicité à l’égard des mammifères à cause de leur activité
cytotoxique et hémolytique. La deuxième contrainte est la dégradation facile des sucres associés à la
génine entraînant souvent la perte d’activité de la molécule. Le caractère hydrophile des saponines
limite leur pénétration à travers la cuticule lipophile des insectes. La complexité structurale des
saponines est une barrière à l’identification exacte des molécules actives et à leur synthèse.
Tunisian Journal of Plant Protection 5: 39-50 . #$ ا:
ا! ت آات.2010 . إ ل،
ا
ارات أن ه)( ا'اد+" ت#, أ. % "! وا أو أآ ر# ات ه اد ي
ء30 ا8#+ .(0 1ة ه)ا ا30' ا4'!5"'ل ا7 ا8ه7 د0 م:'; ا5 و< و ه)ا ا4 ه4+ت إد- .'
A" أ.4+% ا# >?" 4)( ا'اد ودرا# @+5 ءA" و إB !ت4'+: 4'; را5ول ا7ا
G داF 4ء ا ه)( ارا30'; ا+ .)( ا'اد# ا'در8 وأه4F% ا4'ه7 ل ا4C>D E)آ
4#I!; ا: "هJ%K B رةLات اI اM 4' 4!"G ه)( ا'اد.' إذ، !ت4+دH"!ت اG!
V+T " "'; ه)( ا'اد ن4U إن.ةI ا; ا' وت اDP وFرQء اIRح اT 4!'" ;A5 و
.ةI! FرQء اIRح اT 4!'" 8+ ون ا)ي3+آ1م إج هن اQK+ ول ا)يK ا. ;"Gا
ه'م إ1 ا#F'; و5 ه)ا اBء ا30 ا.+>Q! ''K أو8L#'ت ا+3H A% J%K آ' أن ه)( ا'اد
4' ن7 ا+" ا'ادE! ت#, أB ،45%T ات%''ل ات آ5ض ا5 ت ا5ا
اقD "! ات اJ5+ ] آ' أ.'ت+3H اZ5 [% ت ا'اء و+; ا ّ !" # ر: >لD ت+!
@M أ.#!" ه)( ا'اد:G+ ' اهر45+ ; ات# 4'ت ا+K ' أن ا1ات ه)ا وIء اI^
Tunisian Journal of Plant Protection 47 Vol. 5, No. 1, 2010
#F وإ45G ا# _+30 V<` اQI ا4 أم درا:?" @: ة !ت:5' ا4!# ا4% أن اآEإ ذ
."ACا
45%T اد،4و: ،4+I ات% ،ولK آ،تC ،4' :4G آ!'ت
___________________________________________________________________________
LITERATURE CITED
1. Adel, M.M., Sehnal, F., and Jurzysta, M. 2000. 11. Berger, J.M. 2001. Isolation, characterization,
Effect of alfalfa saponins on the mouth and synthesis of bioactive natural products from
Spodoptera littoralis. J. Chem. Ecol. 26: 1065- rainforest flora. Philosophical Doctorate.
1078. Virginia Polytechnic Institute and State
2. Agrell, J., Oleszek, W., Stochmal, A., Olsen, M., University Blacksburg, Virginia, 210 pp.
and Anderson, P. 2003. Herbivore-induced 12. Bruneton, J. 1999. Pharmacognosie phytochimie
responses in alfalfa (Medicago sativa). J. Chem. plantes médicinales. Lavoisier Eds., Paris, 1120
Ecol. 29: 303-320. pp.
3. Appelbaum, S.W., Marco, S., and Birk, Y. 1969. 13. Cafieri, F., Fattorusso, E., and Taglialatela-
Saponins as possible factor of resistance of Scafati, O. 1999. Ectyplasides A-B unique
legume seeds to the attack of insects. J. Agr. triterpene oligoglycosides from the Caribbean
Food. Chem. 17: 618-622. sponge Ectyoplasia ferox. Eur. J. Org. Chem.
4. Armah, C.N., Mackie, A.R., Roy, C., Price, K., 193: 231-238.
Osbourn, A.E., Bowyer, P., and Ladha, S. 1999. 14. Chaieb, I. 2005. Les saponines du Cestrum
The Membrane-permeabilizing effect of parqui nature chimique implications
avenacin A-1 involves the reorganization of physiologiques et potentiel bio-pesticide. Thèse
bilayer cholesterol. Biophys. J. 76: 281-290. de Doctorat en Sciences Agronomiques. Ecole
5. Arnault, C., Harmatha, J., Mauchamp, B., and Supérieure d’Horticulture et d’Elevage de Chott
Salama, K. 1987. Influence of allelochemical Mariem, Tunisia, 157 pp.
substances of the host plant (Allium porrum) on 15. Chaieb, I., Ben Halima-Kamel, M., and Ben
development and molting of Acrolepiosis Hamouda M.H. 2001. Effects of diet addition of
assectella (Lepidoptera). Their role selective Cestrum parquii (Solanaecae) extracts on some
factors. Pages 249-255. In: Insects-Plants. V. Lepidoptera pests: Pieris brassicae (Pieridae) et
Labeyrie, G. Fabers, D. Lachaise, Eds. W Junk Spodoptera littoralis Boisduval (Noctuidae).
Publischer, Netherlands, 448 pp. Med. Fac. Lanbouww. Uni. Gent. 66: 479-480.
6. Arnault, C. and Mauchamp, B. 1985. Ecdysis 16. Chaieb, I., Ben Halima-Kamel, M., and Ben
inhibition in Acrolepiosis assectella larvae by Hamouda M.H. 2004. Modifications cuticulaire
digitonin: antagonistic effect of cholesterol. et protéinique de Spodoptera littoralis
Experientia 41: 1074-1077. Boisduval (Lepidoptera) sous l’action d’une
7. Baqai, F.T., Ali, A., and Ahmad, V.U. 2001.Two alimentation additionnée d’extrait sec de
new spirostanol glycosides from Cestrum Cestrum parquii l'Hérit (Solanaceae). Ann.
parqui. Helv. Chim. Acta 84: 3350-3356. INRAT 77: 119-135.
8. Barbosa, P., Gross, P., Provan, G.J., and 17. Chaieb, I., Ben Halima-Kamel, M., and Ben
Stermiz, F.R. 1990. Allelochemicals in foliage Hamouda, M.H. 2006. Insect growth regulator
of unfavored tree hoss of the gypsy mooth activity of Cestrum parqui saponins: an
Lymantria dispar L. seasonal variation of interaction with cholesterol metabolism.
saponins in Ilex opacea and identification of Commun. Agric. Appl. Biol. Sci. 71: 489-496.
saponin aglycones. J. Chem. Ecol. 16: 1731- 18. Chaieb, I., Ben Hamouda, A., Trabelsi, M., Ben
1738. Halima, M., and Ben Hamouda, M.H. 2009.
9. Barbouche, N., Hajem, B., Lognay G., and Toxicity investigation of Cestrum parqui
Ammar, M. 2001. Contribution à l'étude de saponins to Culex pipiens larvae. Pest Tech. 3:
l'activité biologique d'extraits de feuilles de 73-75.
Cestrum parquii sur le criquet pèlerin 19. Chaieb, I., Trabelsi, M., Ben Halima-Kamel, M.,
Schistocera gregaria. Biotechnol. Agron. Soc. and Ben Hamouda, M.H. 2007. Histological
Environ. 5: 85-90. effects of Cestrum parqui saponins on
10. Berenbaum, M.R. 1995. The Chemistry of Schistocerca gregaria and Spodoptera
defence: theory and practice. Pages 1-16. In: littoralis. J. Biol. Sci. 7: 95-101.
Chemical ecology: the chemistry of biotic 20. Cheeke, P.R. 1971. Nutritional and
interaction Thomas, E., and Meinwald, J., éd. physiological implications of saponins: A
National Academy of Science Washington DC, review. Can. J. Ani. Sci. 51: 621-632.
224 pp.
Tunisian Journal of Plant Protection 48 Vol. 5, No. 1, 2010
21. Chuan-Chun, Z., Shu-Jie, H., Yang, S., Ping- 33. Kreuger, B. and Potter, D.A. 1994. Changes in
Sheng, L., and Xiao-Tian, L. 2003. The saponin and tannins in ripening holly fruits and
synthesis of gracillin and dioscin: two typical effects of fruit consumption on non adapted
representatives of spirostanol glycosides. insect herbivore. Am. Midl. Nat. 132: 183-191.
Carbohy. Res. 338: 721-727. 34. Milgate, J. and Roberts, C.K. 1995. The
22. De Geyter, E., Lambert, E., Geelen, D., and nutritional and the biological significance of
Smagghe, G. 2007. Novel Advances with plant saponin. Nutr. Res. 15: 1223-1249.
saponins as natural insecticides to control pest 35. Mitra, S. and Dungan, S.R. 2000. Micellar
insects. Pest Tech. 1: 96-105. properties of Quillaja saponin. 2. Effect of
23. De Geyter, E., Geelen, D., and Smagghe, G. solubilized cholesterol on solution properties.
2007. First results on the insecticidal action of Coll. Surf. Biointer. 17: 117-133
saponins. Commun. Agric. Appl. Biol. Sci. 72: 36. Nozzolillo, C., Arnason, J.T., Campos, F.,
645-648. Donskov, N., and Jurzysta, M. 1997. Alfalfa
24. Duke, S.O. 1990. Natural pesticides from plants. leaf sapnins and insect resistance. J. Chem.
Pages 511-517. In: Advances in new crops. J. Ecol. 23: 995-1002.
Janick, and J.E. Simon, éd. Timber press, 37. Oleszek, W.A., Hoagland, R., and Zablotowicz,
Portland, Oregon, USA, 540 pp. E. 1999. Ecological significance of plant
25. Febvay, G., Bourgeois, P., and Kermarrec, A. saponins. Pages 451-465. In: Principles and
1985. Antifeedants for attine ant, Acromymex practices in plant ecology allelochemical
octospinosus (Reich) (Hymenoptera- interactions. K.M.M. Dakshini and C.L. Foy,
Formicidae), in several ignam spices Eds. Chemical Rubber Company Press, 608 pp.
(Discoreaceae) cultivated in Antilla. 38. Papadopoulou, K., Melton R.E., Leggett M.,
Agronomie 5: 439-444. Daniels M.J., and Osbourn, A.E. 1999.
26. Gestetener, B., Assa, Y., Henis, Y., Tencer, Y., Compromised disease resistance in saponin-
Rotman, M., Birk, Y., and Bondi, A. 1972. deficient plants. Proc. Nat. Acad. Sci. USA 96:
Interaction of lucerne saponin with sterols. 12923-12928.
Biochem. Biophys. Acta 270: 181-187. 39. Paré, P.W. and Tumlinson, J.H. 1996. Plant
27. Haridas, V., Arntzen, C.J., and Gutterman, J.U. volatile signals in response to herbivore
2001. Avicins, a family of triterpenoid saponins feeding. Fla. Entomol. 79: 93-103.
from Acacia victoriae (Bentham), inhibit 40. Pelah, D., Abramovich, Z., Markus, A., and
activation of nuclear factor-kB by inhibiting Wiesman, Z. 2002. The use of commercial
both its nuclear localization and ability to bind saponin from Quillaja saponaria bark as a
DNA. Proc. Nat. Acad. Sci. USA 98: 11557- natural larvicidal agent against Aedes aegypti
11562. and Culex pipiens. J. Ethnopharmacol 81: 407-
28. Harmatha, J. 2000. Chemo-ecological role of 409.
spirostanol saponins in the interaction between 41. Plasman, V., Braekman, J.C., Daloze, D.,
plants and insects. Pages 129-141. In: Saponin Luhmer, M., Windsor, D., and Pasteels, J.M.
in food, feedstuffs and medicinal plants. W. 2000. Triterpene saponins in the defensive
Olezek and A. Marston, éd, Kluwer Academic secretion of a chrysomelid beetle, Platyphora
Publisher, Netherlands, 304 pp. ligata. J. Nat. Prod. 63: 646-649.
29. Harmatha, J., Mauchamp, B., Arnault, C., and 42. Potter, D.A. and Kimmerer, T.W. 1989.
Salama, K. 1987. Identification of spirostane Inhibition of herbivory on young holly leaves
type saponin in the flowers of leek with evidence for defensive role of saponins.
inhibitory effect on growth of leek-mouth Oecologia 78: 322-329.
larvae. Biochem. System. Ecol. 15: 113-116. 43. Pracros, P. 1988. Mesure de l'activité des
30. Hu, M., Konoki, K., and Tachibana, K. 1996. saponines de la luzerne par les larves du ver de
Cholesterol independent membrane disruption la farine: Tenebrio molitor L. (Coléoptère,
caused by triterpenoid saponins. Biochem. Tenebrionidae). I - Comparaison avec les divers
Biophys. Acta 1299: 252-258. tests biologiques. Agronomie 8: 257-263.
31. Jain, D.C. and Tripathi, A.K. 1999. Insect 44. Rahbe, Y., Febavy, G., and Kermarrec, A. 1988.
feeding deterrent activity of some saponin Foraging activity of attine ant Acromyrex
glycosides. Phytother. Res. 5: 139-141. octospinosus (reich) (Hymnoptera: Fomicidae)
32. Keukens, E.A., De Vrije, T., Van den Boom, C., on resistant susceptible yam varieties. Bul
De Waard, P., Plasman, H.H., Thiel, F., Entomol. Res. 78: 339-349.
Chupin,V., Jongen, W.M., and De Kruijff, B. 45. Serizawa, H., Schinoda, T., and Kawai, A. 2001.
1995. Molecular basis of glycoalkaloid induced Occurrence of a feeding deterrent in Barbarea
membrane disruption. Biochem. Biophys. Acta vulgaris (Brassicales: Brassicaceae), a crucifer
1240: 216-228. unacceptable to the diamondback moth, plutella
xylostella. Appl. Entomol. Zool. 36: 465-470.
-------------------------