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Name: ______________________________________ Date: __________________

Introduction to Microbiology – Virtual API 20E lab

1. Using the API 20E diagnostic tool, identify all unknown organisms.

Unknown Number API 20E Analytical Profile Organism Identification


Number
Organism 1
Organism 2
Organism 3
Organism 4
Organism 5
Organism 6

2. For each organism identified, determine whether or not it would be considered normal enteric
microbiota or an enteric pathogen. If it is considered normal enteric microbiota, also include
under what circumstances this organism could become a pathogen.

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3. Compare and contrast the methods used by microbiologists to identify bacteria. The methods
discussed should include traditional methods used in lab (selective and differential media,
special growth conditions, broths and agars, etc.) as well as the API test, and automated
technologies available, such as Biomerieux’s Vitek system. Use Biomerieux’s website to learn
more about API and Vitek. Your analyses can include things such as time, cost, limitations, etc.
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